Alas1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alas1
Entrez Gene ID	11655
Full Name	aminolevulinic acid synthase 1
Synonyms	ALAS,ALAS-N,Alas-1,Alas-h
General protein information	Preferred Names aminolevulinic acid synthase 1 Names 5-aminolevulinate synthase, nonspecific, mitochondrial 5-aminolevulinic acid synthase 1 aminolevulinate synthase H delta-ALA synthase 1 delta-aminolevulinate synthase 1 succinyl-CoA: glycine C-succinyl transferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_020559.2	NP_065584.2	5-aminolevulinate synthase, nonspecific, mitochondrial precursor
NM_001291835.1	NP_001278764.1	5-aminolevulinate synthase, nonspecific, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ALAS1	XP_001171508.2
Canis lupus familiaris (dog)	ALAS1	XP_533804.3
Bos taurus (cattle)	ALAS1	NP_001094624.1
Danio rerio (zebrafish)	alas1	NP_958444.1
Homo sapiens (human)	ALAS1	NP_000679.1
Mus musculus (house mouse)	Alas1	NP_065584.2
Rattus norvegicus (Norway rat)	Alas1	NP_077810.2
	ALAS1	XP_001090675.1
Gallus gallus (chicken)	ALAS1	NP_001018012.1
Xenopus tropicalis (tropical clawed frog)	alas1	NP_001008072.1

Gene Ontology
Bibliography

Related articles in PubMed

RNAi-mediated silencing of hepatic Alas1 effectively prevents and treats the induced acute attacks in acute intermittent porphyria mice.
Yasuda M, Gan L, Chen B, Kadirvel S, Yu C, Phillips JD, New MI, Liebow A, Fitzgerald K, Querbes W, Desnick RJ
Proceedings of the National Academy of Sciences of the United States of America111(21)7777-82(2014 May)

ALAS1 gene expression is down-regulated by Akt-mediated phosphorylation and nuclear exclusion of FOXO1 by vanadate in diabetic mice.
Oliveri LM, Davio C, Batlle AM, Gerez EN
The Biochemical journal442(2)303-10(2012 Mar)

Functional asymmetry for the active sites of linked 5-aminolevulinate synthase and 8-amino-7-oxononanoate synthase.
Turbeville TD, Zhang J, Adams WC, Hunter GA, Ferreira GC
Archives of biochemistry and biophysics511(1-2)107-17(2011 Jul)

Egr-1 regulates the transcriptional repression of mouse ?-aminolevulinic acid synthase 1 by heme.
Gotoh S, Nakamura T, Kataoka T, Taketani S
Gene472(1-2)28-36(2011 Feb)

Indispensable function for embryogenesis, expression and regulation of the nonspecific form of the 5-aminolevulinate synthase gene in mouse.
Okano S, Zhou L, Kusaka T, Shibata K, Shimizu K, Gao X, Kikuchi Y, Togashi Y, Hosoya T, Takahashi S, Nakajima O, Yamamoto M
Genes to cells : devoted to molecular & cellular mechanisms15(1)77-89(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RNAi-mediated silencing of hepatic Alas1 effectively prevents and treats the induced acute attacks in acute intermittent porphyria mice.
Title: RNAi-mediated silencing of hepatic Alas1 effectively prevents and treats the induced acute attacks in acute intermittent porphyria mice.

Both ALAS1 mRNA and protein content were induced in diabetic animals, accompanied by decreased Akt phosphorylation and increased nuclear FOXO1, PGC-1alpha and FOXO1-PGC-1alpha complex levels.
Title: ALAS1 gene expression is down-regulated by Akt-mediated phosphorylation and nuclear exclusion of FOXO1 by vanadate in diabetic mice.

active site functional asymmetry extends to chimeric ALAS/AONS proteins, which while having a different oligomeric state, exhibit different rates of product release from the two ALAS and two AONS active sites due to the created intermolecular strain
Title: Functional asymmetry for the active sites of linked 5-aminolevulinate synthase and 8-amino-7-oxononanoate synthase.

Egr-1 regulates the transcriptional repression of mouse delta-aminolevulinic acid synthase 1 by heme
Title: Egr-1 regulates the transcriptional repression of mouse ?-aminolevulinic acid synthase 1 by heme.

Mice bearing a homozygous knock-in allele were lethal by embryonic day 8.5, demonstrating that ALAS-N is essential for early embryogenesis.
Title: Indispensable function for embryogenesis, expression and regulation of the nonspecific form of the 5-aminolevulinate synthase gene in mouse.

The following Alas1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alas1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19089	NM_001291835.1 Latest version!	Mus musculus aminolevulinic acid synthase 1 (Alas1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu19089	NM_020559.2 Latest version!	Mus musculus aminolevulinic acid synthase 1 (Alas1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alas1 ( NM_020559.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_065584.2 Mus musculus aminolevulinic acid synthase 1 (Alas1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19089
Clone ID Related Accession (Same CDS sequence)	NM_020559.2 , NM_001291835.1
Accession Version	NM_020559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	5-aminolevulinate synthase, nonspecific, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY129328.1 and AK133283.1. On Nov 20, 2007 this sequence version replaced NM_020559.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.4.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGAGACTG TCGTTCGCCG ATGCCCATTC TTATCCCGAG TCCCTCAGGC CTTTCTGCAG 
AAGGCAGGAA AGTCTCTGTT GTTCTATGCT CAAAACTGCC CCAAGATGAT GGAAGTTGGG 
GCCAAGCCAG CTCCTCGAAC CCTGTCCACA TCAGCTGTCC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAGAA AGAGAAAACT GCCAAGGCCG CAGTCCAGCA GGCTCCTGAC 
GAGTCCCAGA TGGCACAGAC TCCAGACGGC ACACAGCTTC CGTCTGGACA CCCGTCCCCT 
GCTACAAGCC AGGGCTCTGG GAGCAAGTGC CCTTTCCTGG CAGCACAGCT GAGCCAGACA 
GGCAGCAGCG TCTTCCGCAA GGCCAGTCTG GAGCTCCAGG AGGACGTGCA GGAAATGCAT 
GCTGTGAGGA AAGAGGCTGC TCAAAGCCCA GTGCCTCCCA GCCTGGTTAA TGTGAAAACC 
GATGGAGAGG ATCCAAGCCG ACTGCTGAAG AACTTCCAGG ACATCATGCG AAAGCAGAGG 
CCAGAAAGAG TGTCTCATCT TCTTCAGGAT AACTTGCCAA AGTCTGTTTC CACTTTTCAA 
TATGATCATT TCTTTGAGAA GAAAATTGAT GAGAAAAAAA ATGATCATAC CTACCGAGTT 
TTTAAAACGG TGAACCGGAG AGCACAAATC TTCCCGATGG CGGATGACTA CACGGATTCC 
CTCATAACCA AAAAGCAGGT GTCGGTCTGG TGCAGTAATG ACTACCTAGG CATGAGTCGA 
CACCCACGGG TGTGTGGGGC TGTCATGGAG ACTGTGAAAC AGCATGGTGC TGGAGCTGGG 
GGAACTAGGA ATATTTCTGG AACGAGCAAG TTCCACGTAG AACTGGAGCA GGCACTGGCC 
GACCTCCACG GCAAAGATGC GGCGCTCTTG TTCTCTTCCT GTTTTGTGGC CAACGACTCC 
ACCCTCTTCA CCCTGGCTAA GATGATGCCA GGCTGTGAAA TTTACTCTGA TTCCGGGAAC 
CATGCCTCCA TGATCCAAGG GATTCGCAAC AGTCGAGTGC CAAAATATAT CTTCCGCCAC 
AATGACGTTA ACCATCTCAG AGAACTGCTG CAGAGATCTG ACCCCTCAGT CCCCAAGATA 
GTAGCATTTG AAACTGTCCA TTCAATGGAT GGAGCAGTGT GCCCACTGGA AGAGCTGTGT 
GACGTGGCCC ATGAGTTTGG GGCAATCACG TTTGTGGATG AGGTCCATGC AGTGGGGCTC 
TATGGAGCTC GAGGTGGAGG GATCGGTGAT CGGGATGGCG TCATGCCAAA AATGGACATC 
ATCTCTGGGA CGCTTGGTAA AGCGTTCGGC TGTGTTGGAG GATACATTGC CAGCACGAGT 
TTGCTGATCG ACACCGTCCG CTCCTACGCT GCGGGCTTCA TCTTCACCAC CTCCTTGCCA 
CCAATGCTGC TGGCTGGAGC CCTGGAGTCT GTGCGGATCC TGAAGAGCAG CGAGGGGCGC 
GCCCTTCGCC GCCAGCACCA GCGAAATGTC AAGCTTCTGA GGCAGATGCT AATGGACGCT 
GGCCTCCCGG TCATCCACTG TCCGAGTCAC ATCATCCCTG TGCGGGTTGC CGATGCTGCT 
AAGAACACAG AGATCTGTGA TGAGTTGATG ACCAGGCATA ATATCTACGT CCAGGCCATC 
AATTACCCAA CAGTGCCTCG CGGGGAGGAG CTCCTGCGGA TCGCCCCCAC CCCGCATCAC 
ACCCCGCAGA TGATGAACTT CTTCGTGGAG AAGCTGCTGG TCACGTGGAA GCGAGTTGGG 
CTGGAACTTA AGCCACATTC GTCAGCTGAA TGCAACTTCT GCAGGAGGCC CCTGCACTTT 
GAAGTGATGA GCGAAAGAGA GAAAGCCTAT TTCTCAGGCA TGAGCAAGAT GGTGTCTGCC 
CAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065584.2
CDS	192..2120
Translation	METVVRRCPFLSRVPQAFLQKAGKSLLFYAQNCPKMMEVGAKPAPRTLSTSAVHCQQVKETPPANEKEKTAKAAVQQAPDESQMAQTPDGTQLPSGHPSPATSQGSGSKCPFLAAQLSQTGSSVFRKASLELQEDVQEMHAVRKEAAQSPVPPSLVNVKTDGEDPSRLLKNFQDIMRKQRPERVSHLLQDNLPKSVSTFQYDHFFEKKIDEKKNDHTYRVFKTVNRRAQIFPMADDYTDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMETVKQHGAGAGGTRNISGTSKFHVELEQALADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDSGNHASMIQGIRNSRVPKYIFRHNDVNHLRELLQRSDPSVPKIVAFETVHSMDGAVCPLEELCDVAHEFGAITFVDEVHAVGLYGARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSLLIDTVRSYAAGFIFTTSLPPMLLAGALESVRILKSSEGRALRRQHQRNVKLLRQMLMDAGLPVIHCPSHIIPVRVADAAKNTEICDELMTRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNFFVEKLLVTWKRVGLELKPHSSAECNFCRRPLHFEVMSEREKAYFSGMSKMVSAQA

Target ORF information:

RefSeq Version	NM_020559.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus aminolevulinic acid synthase 1 (Alas1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020559.2

ORF Insert Sequence:

ATGGAGACTG TCGTTCGCCG ATGCCCATTC TTATCCCGAG TCCCTCAGGC CTTTCTGCAG 
AAGGCAGGAA AGTCTCTGTT GTTCTATGCT CAAAACTGCC CCAAGATGAT GGAAGTTGGG 
GCCAAGCCAG CTCCTCGAAC CCTGTCCACA TCAGCTGTCC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAGAA AGAGAAAACT GCCAAGGCCG CAGTCCAGCA GGCTCCTGAC 
GAGTCCCAGA TGGCACAGAC TCCAGACGGC ACACAGCTTC CGTCTGGACA CCCGTCCCCT 
GCTACAAGCC AGGGCTCTGG GAGCAAGTGC CCTTTCCTGG CAGCACAGCT GAGCCAGACA 
GGCAGCAGCG TCTTCCGCAA GGCCAGTCTG GAGCTCCAGG AGGACGTGCA GGAAATGCAT 
GCTGTGAGGA AAGAGGCTGC TCAAAGCCCA GTGCCTCCCA GCCTGGTTAA TGTGAAAACC 
GATGGAGAGG ATCCAAGCCG ACTGCTGAAG AACTTCCAGG ACATCATGCG AAAGCAGAGG 
CCAGAAAGAG TGTCTCATCT TCTTCAGGAT AACTTGCCAA AGTCTGTTTC CACTTTTCAA 
TATGATCATT TCTTTGAGAA GAAAATTGAT GAGAAAAAAA ATGATCATAC CTACCGAGTT 
TTTAAAACGG TGAACCGGAG AGCACAAATC TTCCCGATGG CGGATGACTA CACGGATTCC 
CTCATAACCA AAAAGCAGGT GTCGGTCTGG TGCAGTAATG ACTACCTAGG CATGAGTCGA 
CACCCACGGG TGTGTGGGGC TGTCATGGAG ACTGTGAAAC AGCATGGTGC TGGAGCTGGG 
GGAACTAGGA ATATTTCTGG AACGAGCAAG TTCCACGTAG AACTGGAGCA GGCACTGGCC 
GACCTCCACG GCAAAGATGC GGCGCTCTTG TTCTCTTCCT GTTTTGTGGC CAACGACTCC 
ACCCTCTTCA CCCTGGCTAA GATGATGCCA GGCTGTGAAA TTTACTCTGA TTCCGGGAAC 
CATGCCTCCA TGATCCAAGG GATTCGCAAC AGTCGAGTGC CAAAATATAT CTTCCGCCAC 
AATGACGTTA ACCATCTCAG AGAACTGCTG CAGAGATCTG ACCCCTCAGT CCCCAAGATA 
GTAGCATTTG AAACTGTCCA TTCAATGGAT GGAGCAGTGT GCCCACTGGA AGAGCTGTGT 
GACGTGGCCC ATGAGTTTGG GGCAATCACG TTTGTGGATG AGGTCCATGC AGTGGGGCTC 
TATGGAGCTC GAGGTGGAGG GATCGGTGAT CGGGATGGCG TCATGCCAAA AATGGACATC 
ATCTCTGGGA CGCTTGGTAA AGCGTTCGGC TGTGTTGGAG GATACATTGC CAGCACGAGT 
TTGCTGATCG ACACCGTCCG CTCCTACGCT GCGGGCTTCA TCTTCACCAC CTCCTTGCCA 
CCAATGCTGC TGGCTGGAGC CCTGGAGTCT GTGCGGATCC TGAAGAGCAG CGAGGGGCGC 
GCCCTTCGCC GCCAGCACCA GCGAAATGTC AAGCTTCTGA GGCAGATGCT AATGGACGCT 
GGCCTCCCGG TCATCCACTG TCCGAGTCAC ATCATCCCTG TGCGGGTTGC CGATGCTGCT 
AAGAACACAG AGATCTGTGA TGAGTTGATG ACCAGGCATA ATATCTACGT CCAGGCCATC 
AATTACCCAA CAGTGCCTCG CGGGGAGGAG CTCCTGCGGA TCGCCCCCAC CCCGCATCAC 
ACCCCGCAGA TGATGAACTT CTTCGTGGAG AAGCTGCTGG TCACGTGGAA GCGAGTTGGG 
CTGGAACTTA AGCCACATTC GTCAGCTGAA TGCAACTTCT GCAGGAGGCC CCTGCACTTT 
GAAGTGATGA GCGAAAGAGA GAAAGCCTAT TTCTCAGGCA TGAGCAAGAT GGTGTCTGCC 
CAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alas1 ( NM_001291835.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278764.1 Mus musculus aminolevulinic acid synthase 1 (Alas1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19089
Clone ID Related Accession (Same CDS sequence)	NM_020559.2 , NM_001291835.1
Accession Version	NM_001291835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	5-aminolevulinate synthase, nonspecific, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133283.1. On May 2, 2014 this sequence version replaced XM_006511632.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK133283.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGAGACTG TCGTTCGCCG ATGCCCATTC TTATCCCGAG TCCCTCAGGC CTTTCTGCAG 
AAGGCAGGAA AGTCTCTGTT GTTCTATGCT CAAAACTGCC CCAAGATGAT GGAAGTTGGG 
GCCAAGCCAG CTCCTCGAAC CCTGTCCACA TCAGCTGTCC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAGAA AGAGAAAACT GCCAAGGCCG CAGTCCAGCA GGCTCCTGAC 
GAGTCCCAGA TGGCACAGAC TCCAGACGGC ACACAGCTTC CGTCTGGACA CCCGTCCCCT 
GCTACAAGCC AGGGCTCTGG GAGCAAGTGC CCTTTCCTGG CAGCACAGCT GAGCCAGACA 
GGCAGCAGCG TCTTCCGCAA GGCCAGTCTG GAGCTCCAGG AGGACGTGCA GGAAATGCAT 
GCTGTGAGGA AAGAGGCTGC TCAAAGCCCA GTGCCTCCCA GCCTGGTTAA TGTGAAAACC 
GATGGAGAGG ATCCAAGCCG ACTGCTGAAG AACTTCCAGG ACATCATGCG AAAGCAGAGG 
CCAGAAAGAG TGTCTCATCT TCTTCAGGAT AACTTGCCAA AGTCTGTTTC CACTTTTCAA 
TATGATCATT TCTTTGAGAA GAAAATTGAT GAGAAAAAAA ATGATCATAC CTACCGAGTT 
TTTAAAACGG TGAACCGGAG AGCACAAATC TTCCCGATGG CGGATGACTA CACGGATTCC 
CTCATAACCA AAAAGCAGGT GTCGGTCTGG TGCAGTAATG ACTACCTAGG CATGAGTCGA 
CACCCACGGG TGTGTGGGGC TGTCATGGAG ACTGTGAAAC AGCATGGTGC TGGAGCTGGG 
GGAACTAGGA ATATTTCTGG AACGAGCAAG TTCCACGTAG AACTGGAGCA GGCACTGGCC 
GACCTCCACG GCAAAGATGC GGCGCTCTTG TTCTCTTCCT GTTTTGTGGC CAACGACTCC 
ACCCTCTTCA CCCTGGCTAA GATGATGCCA GGCTGTGAAA TTTACTCTGA TTCCGGGAAC 
CATGCCTCCA TGATCCAAGG GATTCGCAAC AGTCGAGTGC CAAAATATAT CTTCCGCCAC 
AATGACGTTA ACCATCTCAG AGAACTGCTG CAGAGATCTG ACCCCTCAGT CCCCAAGATA 
GTAGCATTTG AAACTGTCCA TTCAATGGAT GGAGCAGTGT GCCCACTGGA AGAGCTGTGT 
GACGTGGCCC ATGAGTTTGG GGCAATCACG TTTGTGGATG AGGTCCATGC AGTGGGGCTC 
TATGGAGCTC GAGGTGGAGG GATCGGTGAT CGGGATGGCG TCATGCCAAA AATGGACATC 
ATCTCTGGGA CGCTTGGTAA AGCGTTCGGC TGTGTTGGAG GATACATTGC CAGCACGAGT 
TTGCTGATCG ACACCGTCCG CTCCTACGCT GCGGGCTTCA TCTTCACCAC CTCCTTGCCA 
CCAATGCTGC TGGCTGGAGC CCTGGAGTCT GTGCGGATCC TGAAGAGCAG CGAGGGGCGC 
GCCCTTCGCC GCCAGCACCA GCGAAATGTC AAGCTTCTGA GGCAGATGCT AATGGACGCT 
GGCCTCCCGG TCATCCACTG TCCGAGTCAC ATCATCCCTG TGCGGGTTGC CGATGCTGCT 
AAGAACACAG AGATCTGTGA TGAGTTGATG ACCAGGCATA ATATCTACGT CCAGGCCATC 
AATTACCCAA CAGTGCCTCG CGGGGAGGAG CTCCTGCGGA TCGCCCCCAC CCCGCATCAC 
ACCCCGCAGA TGATGAACTT CTTCGTGGAG AAGCTGCTGG TCACGTGGAA GCGAGTTGGG 
CTGGAACTTA AGCCACATTC GTCAGCTGAA TGCAACTTCT GCAGGAGGCC CCTGCACTTT 
GAAGTGATGA GCGAAAGAGA GAAAGCCTAT TTCTCAGGCA TGAGCAAGAT GGTGTCTGCC 
CAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278764.1
CDS	579..2507
Translation	METVVRRCPFLSRVPQAFLQKAGKSLLFYAQNCPKMMEVGAKPAPRTLSTSAVHCQQVKETPPANEKEKTAKAAVQQAPDESQMAQTPDGTQLPSGHPSPATSQGSGSKCPFLAAQLSQTGSSVFRKASLELQEDVQEMHAVRKEAAQSPVPPSLVNVKTDGEDPSRLLKNFQDIMRKQRPERVSHLLQDNLPKSVSTFQYDHFFEKKIDEKKNDHTYRVFKTVNRRAQIFPMADDYTDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMETVKQHGAGAGGTRNISGTSKFHVELEQALADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDSGNHASMIQGIRNSRVPKYIFRHNDVNHLRELLQRSDPSVPKIVAFETVHSMDGAVCPLEELCDVAHEFGAITFVDEVHAVGLYGARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSLLIDTVRSYAAGFIFTTSLPPMLLAGALESVRILKSSEGRALRRQHQRNVKLLRQMLMDAGLPVIHCPSHIIPVRVADAAKNTEICDELMTRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNFFVEKLLVTWKRVGLELKPHSSAECNFCRRPLHFEVMSEREKAYFSGMSKMVSAQA

Target ORF information:

RefSeq Version	NM_001291835.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus aminolevulinic acid synthase 1 (Alas1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291835.1

ORF Insert Sequence:

ATGGAGACTG TCGTTCGCCG ATGCCCATTC TTATCCCGAG TCCCTCAGGC CTTTCTGCAG 
AAGGCAGGAA AGTCTCTGTT GTTCTATGCT CAAAACTGCC CCAAGATGAT GGAAGTTGGG 
GCCAAGCCAG CTCCTCGAAC CCTGTCCACA TCAGCTGTCC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAGAA AGAGAAAACT GCCAAGGCCG CAGTCCAGCA GGCTCCTGAC 
GAGTCCCAGA TGGCACAGAC TCCAGACGGC ACACAGCTTC CGTCTGGACA CCCGTCCCCT 
GCTACAAGCC AGGGCTCTGG GAGCAAGTGC CCTTTCCTGG CAGCACAGCT GAGCCAGACA 
GGCAGCAGCG TCTTCCGCAA GGCCAGTCTG GAGCTCCAGG AGGACGTGCA GGAAATGCAT 
GCTGTGAGGA AAGAGGCTGC TCAAAGCCCA GTGCCTCCCA GCCTGGTTAA TGTGAAAACC 
GATGGAGAGG ATCCAAGCCG ACTGCTGAAG AACTTCCAGG ACATCATGCG AAAGCAGAGG 
CCAGAAAGAG TGTCTCATCT TCTTCAGGAT AACTTGCCAA AGTCTGTTTC CACTTTTCAA 
TATGATCATT TCTTTGAGAA GAAAATTGAT GAGAAAAAAA ATGATCATAC CTACCGAGTT 
TTTAAAACGG TGAACCGGAG AGCACAAATC TTCCCGATGG CGGATGACTA CACGGATTCC 
CTCATAACCA AAAAGCAGGT GTCGGTCTGG TGCAGTAATG ACTACCTAGG CATGAGTCGA 
CACCCACGGG TGTGTGGGGC TGTCATGGAG ACTGTGAAAC AGCATGGTGC TGGAGCTGGG 
GGAACTAGGA ATATTTCTGG AACGAGCAAG TTCCACGTAG AACTGGAGCA GGCACTGGCC 
GACCTCCACG GCAAAGATGC GGCGCTCTTG TTCTCTTCCT GTTTTGTGGC CAACGACTCC 
ACCCTCTTCA CCCTGGCTAA GATGATGCCA GGCTGTGAAA TTTACTCTGA TTCCGGGAAC 
CATGCCTCCA TGATCCAAGG GATTCGCAAC AGTCGAGTGC CAAAATATAT CTTCCGCCAC 
AATGACGTTA ACCATCTCAG AGAACTGCTG CAGAGATCTG ACCCCTCAGT CCCCAAGATA 
GTAGCATTTG AAACTGTCCA TTCAATGGAT GGAGCAGTGT GCCCACTGGA AGAGCTGTGT 
GACGTGGCCC ATGAGTTTGG GGCAATCACG TTTGTGGATG AGGTCCATGC AGTGGGGCTC 
TATGGAGCTC GAGGTGGAGG GATCGGTGAT CGGGATGGCG TCATGCCAAA AATGGACATC 
ATCTCTGGGA CGCTTGGTAA AGCGTTCGGC TGTGTTGGAG GATACATTGC CAGCACGAGT 
TTGCTGATCG ACACCGTCCG CTCCTACGCT GCGGGCTTCA TCTTCACCAC CTCCTTGCCA 
CCAATGCTGC TGGCTGGAGC CCTGGAGTCT GTGCGGATCC TGAAGAGCAG CGAGGGGCGC 
GCCCTTCGCC GCCAGCACCA GCGAAATGTC AAGCTTCTGA GGCAGATGCT AATGGACGCT 
GGCCTCCCGG TCATCCACTG TCCGAGTCAC ATCATCCCTG TGCGGGTTGC CGATGCTGCT 
AAGAACACAG AGATCTGTGA TGAGTTGATG ACCAGGCATA ATATCTACGT CCAGGCCATC 
AATTACCCAA CAGTGCCTCG CGGGGAGGAG CTCCTGCGGA TCGCCCCCAC CCCGCATCAC 
ACCCCGCAGA TGATGAACTT CTTCGTGGAG AAGCTGCTGG TCACGTGGAA GCGAGTTGGG 
CTGGAACTTA AGCCACATTC GTCAGCTGAA TGCAACTTCT GCAGGAGGCC CCTGCACTTT 
GAAGTGATGA GCGAAAGAGA GAAAGCCTAT TTCTCAGGCA TGAGCAAGAT GGTGTCTGCC 
CAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNAi-mediated silencing of hepatic Alas1 effectively prevents and treats the induced acute attacks in acute intermittent porphyria mice.
Proceedings of the National Academy of Sciences of the United States of America111(21)7777-82(2014 May)
Yasuda M,Gan L,Chen B,Kadirvel S,Yu C,Phillips JD,New MI,Liebow A,Fitzgerald K,Querbes W,Desnick RJ

ALAS1 gene expression is down-regulated by Akt-mediated phosphorylation and nuclear exclusion of FOXO1 by vanadate in diabetic mice.
The Biochemical journal442(2)303-10(2012 Mar)
Oliveri LM,Davio C,Batlle AM,Gerez EN

Functional asymmetry for the active sites of linked 5-aminolevulinate synthase and 8-amino-7-oxononanoate synthase.
Archives of biochemistry and biophysics511(1-2)107-17(2011 Jul)
Turbeville TD,Zhang J,Adams WC,Hunter GA,Ferreira GC

Egr-1 regulates the transcriptional repression of mouse ?-aminolevulinic acid synthase 1 by heme.
Gene472(1-2)28-36(2011 Feb)
Gotoh S,Nakamura T,Kataoka T,Taketani S

Indispensable function for embryogenesis, expression and regulation of the nonspecific form of the 5-aminolevulinate synthase gene in mouse.
Genes to cells : devoted to molecular & cellular mechanisms15(1)77-89(2010 Jan)
Okano S,Zhou L,Kusaka T,Shibata K,Shimizu K,Gao X,Kikuchi Y,Togashi Y,Hosoya T,Takahashi S,Nakajima O,Yamamoto M

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Alas1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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