Alpl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alpl
Entrez Gene ID	11647
Full Name	alkaline phosphatase, liver/bone/kidney
Synonyms	ALP,APTNAP,Akp-2,Akp2,TNAP,TNSALP
General protein information	Preferred Names alkaline phosphatase, liver/bone/kidney Names alkaline phosphatase, tissue-nonspecific isozyme alkaline phosphatase 2, liver
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 D3\|4 70.02 cM
Summary	This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack this enzyme show symptoms of osteomalacia, softening of the bones. In humans, mutations in this gene are associated with hypophosphatasia, an inherited metabolic bone disease in which deficiency of this enzyme inhibits bone mineralization leading to skeletal defects. Mutations in the mouse gene mirror the symptoms of human hypophosphatasia. A pseudogene of this gene is present on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006538498.3	XP_006538561.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_006538497.3	XP_006538560.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_006538500.2	XP_006538563.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
NM_001287172.1	NP_001274101.1	alkaline phosphatase, tissue-nonspecific isozyme preproprotein
XM_006538499.4	XP_006538562.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_006538499.3	XP_006538562.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_017319924.1	XP_017175413.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
NM_007431.3	NP_031457.2	alkaline phosphatase, tissue-nonspecific isozyme preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ALPL	NP_789828.2
Canis lupus familiaris (dog)	ALPL	NP_001184066.1
Rattus norvegicus (Norway rat)	Alpl	NP_037191.1
Gallus gallus (chicken)	ALPL	NP_990691.1
Drosophila melanogaster (fruit fly)	CG8147	NP_649897.4
Homo sapiens (human)	ALPL	NP_000469.3
	ALPL	XP_001109717.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001684	XP_321411.3
Pan troglodytes (chimpanzee)	ALPL	XP_003307915.1
Xenopus tropicalis (tropical clawed frog)	LOC100485467	XP_002943485.2
Mus musculus (house mouse)	Alpl	NP_001274101.1
Danio rerio (zebrafish)	alp3	NP_001265768.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues.
Brun-Heath I, Ermonval M, Chabrol E, Xiao J, Palkovits M, Lyck R, Miller F, Couraud PO, Mornet E, Fonta C
Cell and tissue research343(3)521-36(2011 Mar)

Kinetic analysis of substrate utilization by native and TNAP-, NPP1-, or PHOSPHO1-deficient matrix vesicles.
Ciancaglini P, Yadav MC, Sim?o AM, Narisawa S, Pizauro JM, Farquharson C, Hoylaerts MF, Mill?n JL
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research25(4)716-23(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results are the first to demonstrate a role for ENC1 in the control of osteoblast differentiation. Additionally, the contrasting mineralization phenotypes and transcriptional patterns seen with coordinate knockdown of both ENC1 isoforms vs selective knockdown of 67 kDa ENC1 suggest opposing roles for the isoforms in regulation of osteoblastic differentiation, through effects on Alpl expression and phosphate cellular
Title: Ectodermal-Neural Cortex 1 Isoforms Have Contrasting Effects on MC3T3-E1 Osteoblast Mineralization and Gene Expression.

Data, including data from studies using cells from transgenic/knockout mice, suggest that Med1 plays role in enamel formation; Med1 induces Alpl via stimulation of Notch1 signaling by forming Notch1-RBP-Jk complex on Alpl promoter. (Med1 = mediator complex subunit 1; Alpl = alkaline phosphatase, liver-bone-kidney; Notch1 = Notch gene homolog 1; RBP-Jk = kappa J region recombining binding protein suppressor of hairless)
Title: Mediator 1 contributes to enamel mineralization as a coactivator for Notch1 signaling and stimulates transcription of the alkaline phosphatase gene.

TNAP overexpression in vascular endothelium in mice leads to an unusual course of coronary atherosclerosis and was accompanied by the reduction in body weight and left ventricular ejection fraction.
Title: Overexpression of tissue-nonspecific alkaline phosphatase (TNAP) in endothelial cells accelerates coronary artery disease in a mouse model of familial hypercholesterolemia.

These analyses revealed that TNAP deficient mice present an increased proliferation of neural precursors, an altered neuronal morphology, and an augmented neuronal activity. We found that these alterations were associated with a partial downregulation of the purinergic P2X7 receptor (P2X7R).
Title: Neurodevelopmental alterations and seizures developed by mouse model of infantile hypophosphatasia are associated with purinergic signalling deregulation.

Despite similar deficiencies in alkaline phosphatase, Alpl(-/-) mice develop craniosynostosis and a brachycephalic/acrocephalic craniofacial shape of variable penetrance.
Title: Bone mineralization-dependent craniosynostosis and craniofacial shape abnormalities in the mouse model of infantile hypophosphatasia.

The following Alpl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alpl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20341	NM_001287172.1 Latest version!	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu20341	NM_007431.3 Latest version!	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu20341	XM_006538497.3 Latest version!	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu20341	XM_006538498.3 Latest version!	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu20341	XM_017319924.1 Latest version!	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu20341	XM_006538500.2 Latest version!	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu20341	XM_006538499.4 Latest version!	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu20341	XM_006538499.3	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alpl ( NM_001287172.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001274101.1 Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20341
Clone ID Related Accession (Same CDS sequence)	XM_006538498.3 , XM_006538497.3 , XM_006538500.2 , NM_001287172.1 , XM_006538499.4 , XM_006538499.3 , XM_017319924.1 , NM_007431.3
Accession Version	NM_001287172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	alkaline phosphatase, tissue-nonspecific isozyme preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN539670.1, AL807764.9, AK167023.1 and AL805954.9. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK076459.1, X13409.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274101.1
CDS	269..1843
Translation	MISPFLVLAIGTCLTNSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAGSGSAPSPGALLLPLAVLSLRTLF

Target ORF information:

RefSeq Version	NM_001287172.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287172.1

ORF Insert Sequence:

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpl ( NM_007431.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031457.2 Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20341
Clone ID Related Accession (Same CDS sequence)	XM_006538498.3 , XM_006538497.3 , XM_006538500.2 , NM_001287172.1 , XM_006538499.4 , XM_006538499.3 , XM_017319924.1 , NM_007431.3
Accession Version	NM_007431.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	alkaline phosphatase, tissue-nonspecific isozyme preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL807764.9, AK164002.1, AK167023.1 and AL805954.9. On Dec 4, 2013 this sequence version replaced NM_007431.2. Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK076459.1, AK167023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031457.2
CDS	244..1818
Translation	MISPFLVLAIGTCLTNSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAGSGSAPSPGALLLPLAVLSLRTLF

Target ORF information:

RefSeq Version	NM_007431.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007431.3

ORF Insert Sequence:

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpl ( XM_006538498.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538561.1 Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu20341
Clone ID Related Accession (Same CDS sequence)	XM_006538498.3 , XM_006538497.3 , XM_006538500.2 , NM_001287172.1 , XM_006538499.4 , XM_006538499.3 , XM_017319924.1 , NM_007431.3
Accession Version	XM_006538498.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538561.1
CDS	211..1785
Translation	MISPFLVLAIGTCLTNSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAGSGSAPSPGALLLPLAVLSLRTLF

Target ORF information:

RefSeq Version	XM_006538498.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538498.3

ORF Insert Sequence:

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpl ( XM_006538497.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538560.1 Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20341
Clone ID Related Accession (Same CDS sequence)	XM_006538498.3 , XM_006538497.3 , XM_006538500.2 , NM_001287172.1 , XM_006538499.4 , XM_006538499.3 , XM_017319924.1 , NM_007431.3
Accession Version	XM_006538497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538560.1
CDS	190..1764
Translation	MISPFLVLAIGTCLTNSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAGSGSAPSPGALLLPLAVLSLRTLF

Target ORF information:

RefSeq Version	XM_006538497.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538497.3

ORF Insert Sequence:

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpl ( XM_006538500.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538563.1 Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu20341
Clone ID Related Accession (Same CDS sequence)	XM_006538498.3 , XM_006538497.3 , XM_006538500.2 , NM_001287172.1 , XM_006538499.4 , XM_006538499.3 , XM_017319924.1 , NM_007431.3
Accession Version	XM_006538500.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538563.1
CDS	534..2108
Translation	MISPFLVLAIGTCLTNSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAGSGSAPSPGALLLPLAVLSLRTLF

Target ORF information:

RefSeq Version	XM_006538500.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538500.2

ORF Insert Sequence:

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpl ( XM_006538499.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538562.1 Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu20341
Clone ID Related Accession (Same CDS sequence)	XM_006538498.3 , XM_006538497.3 , XM_006538500.2 , NM_001287172.1 , XM_006538499.4 , XM_006538499.3 , XM_017319924.1 , NM_007431.3
Accession Version	XM_006538499.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538499.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538562.1
CDS	116..1690
Translation	MISPFLVLAIGTCLTNSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAGSGSAPSPGALLLPLAVLSLRTLF

Target ORF information:

RefSeq Version	XM_006538499.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538499.3

ORF Insert Sequence:

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpl ( XM_017319924.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175413.1 Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X3, mRNA.

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CloneID	OMu20341
Clone ID Related Accession (Same CDS sequence)	XM_006538498.3 , XM_006538497.3 , XM_006538500.2 , NM_001287172.1 , XM_006538499.4 , XM_006538499.3 , XM_017319924.1 , NM_007431.3
Accession Version	XM_017319924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175413.1
CDS	387..1961
Translation	MISPFLVLAIGTCLTNSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAGSGSAPSPGALLLPLAVLSLRTLF

Target ORF information:

RefSeq Version	XM_017319924.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319924.1

ORF Insert Sequence:

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpl ( XM_006538499.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538562.1 Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu20341
Clone ID Related Accession (Same CDS sequence)	XM_006538498.3 , XM_006538497.3 , XM_006538500.2 , NM_001287172.1 , XM_006538499.4 , XM_006538499.3 , XM_017319924.1 , NM_007431.3
Accession Version	XM_006538499.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538499.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538562.1
CDS	117..1691
Translation	MISPFLVLAIGTCLTNSFVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAGSGSAPSPGALLLPLAVLSLRTLF

Target ORF information:

RefSeq Version	XM_006538499.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538499.4

ORF Insert Sequence:

ATGATCTCAC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC TTTTGTGCCA 
GAGAAAGAGA GAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAGCTCAA CACCAATGTA GCCAAGAATG TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT AACCGCTGCC CGAATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACCCGGCT GGAGATGGAC AAATTCCCCT TTGTGGCCCT CTCCAAGACA 
TATAACACCA ACGCTCAGGT CCCTGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC AGTGGGAGTG AGCGCAGCCA CAGAGCGCAC GCGATGCAAC 
ACCACTCAGG GCAATGAGGT CACATCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATTG TGACTACCAC TCGGGTGAAC CACGCCACAC CCAGTGCAGC CTACGCACAC 
TCGGCCGATC GGGACTGGTA CTCGGATAAC GAGATGCCAC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCATATCA GCTAATGCAC AATATCAAGG ATATCGACGT GATCATGGGT 
GGCGGCCGGA AATACATGTA CCCGAAGAAC AGAACTGATG TGGAATACGA ACTGGATGAG 
AAGGCCAGGG GTACAAGGCT AGATGGCCTG GATCTCATCA GTATTTGGAA GAGCTTTAAA 
CCCAGACACA AGCATTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAATTGAAT 
CGGAACAACC TGACTGACCC TTCGCTCTCC GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACCAAAAACC TCAAAGGCTT CTTCTTGCTG GTGGAAGGAG GCAGGATTGA CCACGGACAT 
CATGAGGGTA AGGCCAAGCA GGCTCTGCAT GAAGCAGTGG AGATGGACCA GGCCATTGGC 
AAGGCAGGCG CCATGACATC CCAGAAAGAC ACCTTGACTG TGGTTACTGC TGATCATTCC 
CACGTTTTCA CATTCGGTGG ATACACCCCC CGGGGCAACT CCATCTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACGGCCATCC TATATGGTAA CGGGCCTGGC 
TACAAGGTGG TGGACGGTGA ACGGGAAAAT GTCTCCATGG TAGATTACGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT TCCCCTGCGC CATGAGACCC ACGGTGGAGA AGACGTGGCG 
GTCTTTGCCA AGGGCCCGAT GGCACACCTG CTTCACGGCG TCCATGAGCA GAACTACATT 
CCCCATGTGA TGGCGTATGC CTCCTGCATT GGGGCCAACC TTGACCACTG TGCCTGGGCC 
GGCTCTGGGA GCGCACCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTGT GCTCTCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues.
Cell and tissue research343(3)521-36(2011 Mar)
Brun-Heath I,Ermonval M,Chabrol E,Xiao J,Palkovits M,Lyck R,Miller F,Couraud PO,Mornet E,Fonta C

Kinetic analysis of substrate utilization by native and TNAP-, NPP1-, or PHOSPHO1-deficient matrix vesicles.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research25(4)716-23(2010 Apr)
Ciancaglini P,Yadav MC,Sim?o AM,Narisawa S,Pizauro JM,Farquharson C,Hoylaerts MF,Mill?n JL

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Alpl cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報