Akap3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akap3
Entrez Gene ID	11642
Full Name	A kinase (PRKA) anchor protein 3
Synonyms	Prka3
General protein information	Preferred Names A kinase (PRKA) anchor protein 3 Names A-kinase anchor protein 3 A-kinase anchor protein 110 kDa AKAP 110 AKAP-3 Protein kinase A-anchoring protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_009650.2	NP_033780.2	A-kinase anchor protein 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Akap3	NP_033780.2
Pan troglodytes (chimpanzee)	AKAP3	XP_001159471.1
	AKAP3	XP_001101659.1
Canis lupus familiaris (dog)	AKAP3	XP_005637329.1
Bos taurus (cattle)	AKAP3	NP_001094509.1
Rattus norvegicus (Norway rat)	Akap3	NP_001005557.1
Homo sapiens (human)	AKAP3	NP_006413.3

Gene Ontology
Bibliography

Related articles in PubMed

CFAP157 is a murine downstream effector of FOXJ1 that is specifically required for flagellum morphogenesis and sperm motility.
Weidemann M, Schuster-Gossler K, Stauber M, Wrede C, Hegermann J, Ott T, Boldt K, Beyer T, Serth K, Kremmer E, Blum M, Ueffing M, Gossler A
Development (Cambridge, England)143(24)4736-4748(2016 12)

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Cui W, Dai X, Marcho C, Han Z, Zhang K, Tremblay KD, Mager J
Scientific reports637396(2016 Nov)

Interactome of the negative regulator of nuclear import BRCA1-binding protein 2.
Fatima S, Wagstaff KM, Loveland KL, Jans DA
Scientific reports59459(2015 Mar)

Sperm-specific AKAP3 is a dual-specificity anchoring protein that interacts with both protein kinase a regulatory subunits via conserved N-terminal amphipathic peptides.
Xu K, Qi H
Molecular reproduction and development81(7)595-607(2014 Jul)

AKAP3 synthesis is mediated by RNA binding proteins and PKA signaling during mouse spermiogenesis.
Xu K, Yang L, Zhao D, Wu Y, Qi H
Biology of reproduction90(6)119(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studied PKA subunit isoform expression in meiotic and post-meiotic mouse spermatogenic cells, and found sperm-specific AKAP3 can be found in the cytoplasm of elongating spermatids and interacts with RIalpha.
Title: Sperm-specific AKAP3 is a dual-specificity anchoring protein that interacts with both protein kinase a regulatory subunits via conserved N-terminal amphipathic peptides.

We analyzed the expression of sperm-specific Akap3 and the potential regulatory factors of its protein synthesis during mouse spermiogenesis.
Title: AKAP3 synthesis is mediated by RNA binding proteins and PKA signaling during mouse spermiogenesis.

The following Akap3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akap3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15716	NM_009650.2 Latest version!	Mus musculus A kinase (PRKA) anchor protein 3 (Akap3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akap3 ( NM_009650.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033780.2 Mus musculus A kinase (PRKA) anchor protein 3 (Akap3), mRNA.

Price & Availability↑

CloneID	OMu15716
Clone ID Related Accession (Same CDS sequence)	NM_009650.2
Accession Version	NM_009650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	A-kinase anchor protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN837067.1, AF093406.1 and BC100458.1. On Nov 16, 2007 this sequence version replaced NM_009650.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF093406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATA GGGTTGACTG GTTACAAAGT CAAAGTGGCG TTTGCAAAGT TGGTGTCTAT 
TCACCTGGAG ACAACCAACA CCAAGACTGG AAAATGGACA CATCAACAGA TCCTGTCCGA 
GTGCTCAGCT GGCTCCGCAA AGACCTGGAG AAAAGTACAG CGGGCTTCCA GGACTCGAGG 
TTCAAGCCTG GAGAGTCATC GTTTGTGGAG GAAGTGGCTT ACCCAGTGGA CCAACGAAAA 
GGTTTCTGTG TTGATTATTA CAATACCACC AACAAGGGCA GTCCAGGAAG ATTGCATTTT 
GAGATGTCTC ACAAGGAGAA CCCTTCCCAG GGCCTCATTT CCCATGTTGG TAATGGGGGT 
TCCATAGACG AAGTTTCCTT CTATGCCAAC CGCCTCACAA ACCTAGTGAT CGCCATGGCC 
CGAAAGGAGA TCAATGAGAA GATCCACGGC GCTGAAAACA AATGTGTCCA TCAGTCATTG 
TATATGGGGG ATGAGCCCAC ACCCCACAAA AGCTTGAGTA CAGTGGCCTC TGAGCTCGTG 
AACGAGACAG TCACCGCATG TTCCAAGAAC ATTTCCAGTG ACAAAGCTCC CGGCTCTGGA 
GACAGGGCCT CGGGGTCGTC ACAGGCCCCT GGTCTAAGAT ACACGAGCAC TCTGAAGATC 
AAGGAGAGTA CAAAGGAAGG CAAGTGTCCA GACGACAAGC CCGGCACTAA GAAGTCTTTC 
TTCTATAAGG AAGTGTTTGA GTCCCGGAAT GCAGGAGATG CCAAGGAGGG CGGGAGGTCC 
TTACCAGGAG ATCAAAAACT GTTCAGGACC AGCCCCGACA ACAGGCCTGA TGACTTTTCA 
AACTCTATCA GTCAAGGGAT CATGACCTAC GCCAACAGCG TGGTGTCTGA CATGATGGTC 
TCCATCATGA AGACGCTGAA GATCCAGGTG AAGGACACAA CCATCGCCAC GATTCTGCTG 
AAGAAGGTGC TGATGAAGCA CGCAAAGGAG GTTGTCTCCG ACCTCATCGA CTCCTTCATG 
AAGAACCTCC ACGGCGTCAC GGGAAGCCTC ATGACTGACA CAGACTTTGT CTCGGCCGTG 
AAACGAAGTT TTTTTTCTCA TGGAAGCCAA AAGGCCACAG ATATCATGGA TGCCATGCTG 
GGCAAGCTAT ACAATGTGAT GTTTGCCAAG AAATTCCCTG AGAACATCCG GAGAGCCAGG 
GACAAGTCGG AGAGTTACTC CCTTATCTCC ACGAAATCAC GGGCTGGTGA CCCAAAGCTC 
TCAAATTTGA ACTTTGCGAT GAAGTCAGAA TCAAAGCTGA AAGAAAATTT GTTTTCTACA 
TGCAAACTAG AGAAAGAGAA GACGTGTGCC GAAACTCTGG GTGAGCATAT TATTAAGGAG 
GGACTGCACA TGTGGCACAA GAGTCAGCAA AAATCTCCTG GCTTGGAGCG TGCCGCAAAA 
CTGGGTAACG CTCCACAGGA GGTCTCCTTT GAGTGCCCAG ATCCTTGTGA GGCAAACCCT 
CCTCACCAAC CTCAGCCACC AGAGAATTTT GCAAATTTTA TGTGTGACTC AGACTCCTGG 
GCCAAGGACC TGATTGTATC CGCCCTGCTT CTGATTCAGT ATCACCTGGC ACAGGGAGGA 
AAGATGGATG CTCAGAGCTT CCTGGAAGCT GCTGCCAGCA CCAATTTTCC CACCAACAAG 
CCACCTCCTC CTTCTCCTGT AGTTCAGGAT GAGTGCAAAC TTAAGTCTCC TCCCCACAAG 
ATATGTGACC AAGAACAAAC AGAAAAGAAA GATCTGATGA GTGTCATCTT CAATTTTATC 
CGGAACTTAC TCAGCGAGAC CATATTCAAG AGTAGCCGTA ACTGTGAATC CAATGTGCAT 
GAGCAGAACA CTCAGGAAGA AGAGATACAC CCGTGTGAAA GGCCTAAGAC TCCATGTGAA 
AGGCCTATTA CCCCGCCTGC CCCGAAATTC TGTGAGGATG AGGAGGCCAC TGGTGGTGCC 
TTATCTGGGC TAACCAAGAT GGTTGCCAAC CAGCTAGACA ACTGTATGAA TGGGCAGATG 
GTGGAGCACC TGATGGACTC GGTGATGAAG TTATGCCTCA TTATTGCCAA GTCCTGTGAC 
TCTCCCCTGT CGGAGCTGGG AGAGGAAAAG TGTGGGGATG CCAGCCGGCC AAATTCTGCC 
TTCCCAGATA ACTTATATGA GTGCCTACCA GTCAAGGGCA CAGGGACAGC TGAAGCCCTC 
CTGCAGAACG CCTACCTCAC CATCCATAAT GAACTGAGAG GTTTGTCAGG ACAGCCCCCC 
GAGGGCTGTG AAATCCCCAA GGTGATCGTC AGCAACCACA ATCTGGCTGA CACCGTTCAG 
AACAAGCAAC TGCAAGCTGT CCTTCAGTGG GTGGCTGCCT CAGAGCTCAA TGTCCCTATT 
TTGTACTTTG CTGGTGACGA TGAAGGAATC CAGGAGAAGC TGCTTCAGCT CTCAGCCACT 
GCCGTGGAGA AAGGCCGCAG CGTCGGGGAG GTTCTGCAGT CGGTGCTGAG GTACGAGAAG 
GAGCGACAGC TGGATGAAGC AGTGGGAAAT GTCACGCGGC TGCAGCTGCT GGACTGGCTG 
ATGGCAAACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033780.2
CDS	366..2960
Translation	MADRVDWLQSQSGVCKVGVYSPGDNQHQDWKMDTSTDPVRVLSWLRKDLEKSTAGFQDSRFKPGESSFVEEVAYPVDQRKGFCVDYYNTTNKGSPGRLHFEMSHKENPSQGLISHVGNGGSIDEVSFYANRLTNLVIAMARKEINEKIHGAENKCVHQSLYMGDEPTPHKSLSTVASELVNETVTACSKNISSDKAPGSGDRASGSSQAPGLRYTSTLKIKESTKEGKCPDDKPGTKKSFFYKEVFESRNAGDAKEGGRSLPGDQKLFRTSPDNRPDDFSNSISQGIMTYANSVVSDMMVSIMKTLKIQVKDTTIATILLKKVLMKHAKEVVSDLIDSFMKNLHGVTGSLMTDTDFVSAVKRSFFSHGSQKATDIMDAMLGKLYNVMFAKKFPENIRRARDKSESYSLISTKSRAGDPKLSNLNFAMKSESKLKENLFSTCKLEKEKTCAETLGEHIIKEGLHMWHKSQQKSPGLERAAKLGNAPQEVSFECPDPCEANPPHQPQPPENFANFMCDSDSWAKDLIVSALLLIQYHLAQGGKMDAQSFLEAAASTNFPTNKPPPPSPVVQDECKLKSPPHKICDQEQTEKKDLMSVIFNFIRNLLSETIFKSSRNCESNVHEQNTQEEEIHPCERPKTPCERPITPPAPKFCEDEEATGGALSGLTKMVANQLDNCMNGQMVEHLMDSVMKLCLIIAKSCDSPLSELGEEKCGDASRPNSAFPDNLYECLPVKGTGTAEALLQNAYLTIHNELRGLSGQPPEGCEIPKVIVSNHNLADTVQNKQLQAVLQWVAASELNVPILYFAGDDEGIQEKLLQLSATAVEKGRSVGEVLQSVLRYEKERQLDEAVGNVTRLQLLDWLMANL

Target ORF information:

RefSeq Version	NM_009650.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus A kinase (PRKA) anchor protein 3 (Akap3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009650.2

ORF Insert Sequence:

ATGGCGGATA GGGTTGACTG GTTACAAAGT CAAAGTGGCG TTTGCAAAGT TGGTGTCTAT 
TCACCTGGAG ACAACCAACA CCAAGACTGG AAAATGGACA CATCAACAGA TCCTGTCCGA 
GTGCTCAGCT GGCTCCGCAA AGACCTGGAG AAAAGTACAG CGGGCTTCCA GGACTCGAGG 
TTCAAGCCTG GAGAGTCATC GTTTGTGGAG GAAGTGGCTT ACCCAGTGGA CCAACGAAAA 
GGTTTCTGTG TTGATTATTA CAATACCACC AACAAGGGCA GTCCAGGAAG ATTGCATTTT 
GAGATGTCTC ACAAGGAGAA CCCTTCCCAG GGCCTCATTT CCCATGTTGG TAATGGGGGT 
TCCATAGACG AAGTTTCCTT CTATGCCAAC CGCCTCACAA ACCTAGTGAT CGCCATGGCC 
CGAAAGGAGA TCAATGAGAA GATCCACGGC GCTGAAAACA AATGTGTCCA TCAGTCATTG 
TATATGGGGG ATGAGCCCAC ACCCCACAAA AGCTTGAGTA CAGTGGCCTC TGAGCTCGTG 
AACGAGACAG TCACCGCATG TTCCAAGAAC ATTTCCAGTG ACAAAGCTCC CGGCTCTGGA 
GACAGGGCCT CGGGGTCGTC ACAGGCCCCT GGTCTAAGAT ACACGAGCAC TCTGAAGATC 
AAGGAGAGTA CAAAGGAAGG CAAGTGTCCA GACGACAAGC CCGGCACTAA GAAGTCTTTC 
TTCTATAAGG AAGTGTTTGA GTCCCGGAAT GCAGGAGATG CCAAGGAGGG CGGGAGGTCC 
TTACCAGGAG ATCAAAAACT GTTCAGGACC AGCCCCGACA ACAGGCCTGA TGACTTTTCA 
AACTCTATCA GTCAAGGGAT CATGACCTAC GCCAACAGCG TGGTGTCTGA CATGATGGTC 
TCCATCATGA AGACGCTGAA GATCCAGGTG AAGGACACAA CCATCGCCAC GATTCTGCTG 
AAGAAGGTGC TGATGAAGCA CGCAAAGGAG GTTGTCTCCG ACCTCATCGA CTCCTTCATG 
AAGAACCTCC ACGGCGTCAC GGGAAGCCTC ATGACTGACA CAGACTTTGT CTCGGCCGTG 
AAACGAAGTT TTTTTTCTCA TGGAAGCCAA AAGGCCACAG ATATCATGGA TGCCATGCTG 
GGCAAGCTAT ACAATGTGAT GTTTGCCAAG AAATTCCCTG AGAACATCCG GAGAGCCAGG 
GACAAGTCGG AGAGTTACTC CCTTATCTCC ACGAAATCAC GGGCTGGTGA CCCAAAGCTC 
TCAAATTTGA ACTTTGCGAT GAAGTCAGAA TCAAAGCTGA AAGAAAATTT GTTTTCTACA 
TGCAAACTAG AGAAAGAGAA GACGTGTGCC GAAACTCTGG GTGAGCATAT TATTAAGGAG 
GGACTGCACA TGTGGCACAA GAGTCAGCAA AAATCTCCTG GCTTGGAGCG TGCCGCAAAA 
CTGGGTAACG CTCCACAGGA GGTCTCCTTT GAGTGCCCAG ATCCTTGTGA GGCAAACCCT 
CCTCACCAAC CTCAGCCACC AGAGAATTTT GCAAATTTTA TGTGTGACTC AGACTCCTGG 
GCCAAGGACC TGATTGTATC CGCCCTGCTT CTGATTCAGT ATCACCTGGC ACAGGGAGGA 
AAGATGGATG CTCAGAGCTT CCTGGAAGCT GCTGCCAGCA CCAATTTTCC CACCAACAAG 
CCACCTCCTC CTTCTCCTGT AGTTCAGGAT GAGTGCAAAC TTAAGTCTCC TCCCCACAAG 
ATATGTGACC AAGAACAAAC AGAAAAGAAA GATCTGATGA GTGTCATCTT CAATTTTATC 
CGGAACTTAC TCAGCGAGAC CATATTCAAG AGTAGCCGTA ACTGTGAATC CAATGTGCAT 
GAGCAGAACA CTCAGGAAGA AGAGATACAC CCGTGTGAAA GGCCTAAGAC TCCATGTGAA 
AGGCCTATTA CCCCGCCTGC CCCGAAATTC TGTGAGGATG AGGAGGCCAC TGGTGGTGCC 
TTATCTGGGC TAACCAAGAT GGTTGCCAAC CAGCTAGACA ACTGTATGAA TGGGCAGATG 
GTGGAGCACC TGATGGACTC GGTGATGAAG TTATGCCTCA TTATTGCCAA GTCCTGTGAC 
TCTCCCCTGT CGGAGCTGGG AGAGGAAAAG TGTGGGGATG CCAGCCGGCC AAATTCTGCC 
TTCCCAGATA ACTTATATGA GTGCCTACCA GTCAAGGGCA CAGGGACAGC TGAAGCCCTC 
CTGCAGAACG CCTACCTCAC CATCCATAAT GAACTGAGAG GTTTGTCAGG ACAGCCCCCC 
GAGGGCTGTG AAATCCCCAA GGTGATCGTC AGCAACCACA ATCTGGCTGA CACCGTTCAG 
AACAAGCAAC TGCAAGCTGT CCTTCAGTGG GTGGCTGCCT CAGAGCTCAA TGTCCCTATT 
TTGTACTTTG CTGGTGACGA TGAAGGAATC CAGGAGAAGC TGCTTCAGCT CTCAGCCACT 
GCCGTGGAGA AAGGCCGCAG CGTCGGGGAG GTTCTGCAGT CGGTGCTGAG GTACGAGAAG 
GAGCGACAGC TGGATGAAGC AGTGGGAAAT GTCACGCGGC TGCAGCTGCT GGACTGGCTG 
ATGGCAAACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CFAP157 is a murine downstream effector of FOXJ1 that is specifically required for flagellum morphogenesis and sperm motility.
Development (Cambridge, England)143(24)4736-4748(2016 12)
Weidemann M,Schuster-Gossler K,Stauber M,Wrede C,Hegermann J,Ott T,Boldt K,Beyer T,Serth K,Kremmer E,Blum M,Ueffing M,Gossler A

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Scientific reports637396(2016 Nov)
Cui W,Dai X,Marcho C,Han Z,Zhang K,Tremblay KD,Mager J

Interactome of the negative regulator of nuclear import BRCA1-binding protein 2.
Scientific reports59459(2015 Mar)
Fatima S,Wagstaff KM,Loveland KL,Jans DA

Sperm-specific AKAP3 is a dual-specificity anchoring protein that interacts with both protein kinase a regulatory subunits via conserved N-terminal amphipathic peptides.
Molecular reproduction and development81(7)595-607(2014 Jul)
Xu K,Qi H

AKAP3 synthesis is mediated by RNA binding proteins and PKA signaling during mouse spermiogenesis.
Biology of reproduction90(6)119(2014 Jun)
Xu K,Yang L,Zhao D,Wu Y,Qi H

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Akap3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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