Ak1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ak1
Entrez Gene ID	11636
Full Name	adenylate kinase 1
Synonyms	Ak-1,B430205N08Rik
General protein information	Preferred Names adenylate kinase 1 Names adenylate kinase isoenzyme 1 cytosolic adenylate kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 B\|2 22.09 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006497624.3	XP_006497687.1	adenylate kinase isoenzyme 1 isoform X2
XM_006497624.2	XP_006497687.1	adenylate kinase isoenzyme 1 isoform X1
NM_001198791.1	NP_001185720.1	adenylate kinase isoenzyme 1 isoform 2
NM_001198792.1	NP_001185721.1	adenylate kinase isoenzyme 1 isoform 2
XM_030246809.1	XP_030102669.1	adenylate kinase isoenzyme 1 isoform X1
NM_001198790.1	NP_001185719.1	adenylate kinase isoenzyme 1 isoform 2
NM_021515.3	NP_067490.1	adenylate kinase isoenzyme 1 isoform 1
XM_017315020.1	XP_017170509.1	adenylate kinase isoenzyme 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AK1	NP_001124519.1
Bos taurus (cattle)	AK1	NP_001013600.1
Arabidopsis thaliana (thale cress)	AT4G25280	NP_194258.2
Homo sapiens (human)	AK1	NP_000467.1
	AK1	XP_002800218.1
Gallus gallus (chicken)	AK1	NP_990440.1
Mus musculus (house mouse)	Ak1	NP_067490.1
Rattus norvegicus (Norway rat)	Ak1	NP_077325.2
Xenopus tropicalis (tropical clawed frog)	ak1	NP_001006817.1
Pan troglodytes (chimpanzee)	AK1	XP_003951582.1
Danio rerio (zebrafish)	ak1	NP_001003993.1
Drosophila melanogaster (fruit fly)	Adk1	NP_729792.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009317	XP_309986.3
Caenorhabditis elegans (roundworm)	F38B2.4	NP_001257141.1

Gene Ontology
Bibliography

Related articles in PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Rutledge EA, Benazet JD, McMahon AP
Development (Cambridge, England)144(17)3177-3188(2017 09)

Tpbpa-Cre-mediated deletion of TFAP2C leads to deregulation of Cdkn1a, Akt1 and the ERK pathway, causing placental growth arrest.
Sharma N, Kubaczka C, Kaiser S, Nettersheim D, Mughal SS, Riesenberg S, H?lzel M, Winterhager E, Schorle H
Development (Cambridge, England)143(5)787-98(2016 Mar)

Adenine nucleotide metabolism and a role for AMP in modulating flagellar waveforms in mouse sperm.
Vadnais ML, Cao W, Aghajanian HK, Haig-Ladewig L, Lin AM, Al-Alao O, Gerton GL
Biology of reproduction90(6)128(2014 Jun)

CaMKI? regulates AMP kinase-dependent, TORC-1-independent autophagy during lipopolysaccharide-induced acute lung neutrophilic inflammation.
Guo L, Stripay JL, Zhang X, Collage RD, Hulver M, Carchman EH, Howell GM, Zuckerbraun BS, Lee JS, Rosengart MR
Journal of immunology (Baltimore, Md. : 1950)190(7)3620-8(2013 Apr)

Conditional deletion of pten leads to defects in nerve innervation and neuronal survival in inner ear development.
Kim HJ, Woo HM, Ryu J, Bok J, Kim JW, Choi SB, Park MH, Park HY, Koo SK
PloS one8(2)e55609(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In addition to AK1 and AK2, which we previously demonstrated are present in outer dense fibers and mitochondrial sheath of the mouse sperm tail, we show that another AK, AK8, is present in a third flagellar compartment, the axoneme.
Title: Adenine nucleotide metabolism and a role for AMP in modulating flagellar waveforms in mouse sperm.

CaMKIalpha regulates AMP kinase-dependent, TORC-1-independent autophagy during lipopolysaccharide-induced acute lung neutrophilic inflammation.
Title: CaMKI? regulates AMP kinase-dependent, TORC-1-independent autophagy during lipopolysaccharide-induced acute lung neutrophilic inflammation.

identified hitherto unrecognized soluble forms of AK1 and NTPDase1/CD39 that contribute in the active cycling between the principal platelet-recruiting agent ADP and other circulating nucleotides
Title: Metabolism of circulating ADP in the bloodstream is mediated via integrated actions of soluble adenylate kinase-1 and NTPDase1/CD39 activities.

Creatine kinase (CK) and adenylate kinase (AK) are especially important in facilitating energy metabolism during very high energy demands.
Title: Gated dynamic 31P MRS shows reduced contractile phosphocreatine breakdown in mice deficient in cytosolic creatine kinase and adenylate kinase.

Adenylate kinase-1 knockout mice have a normal capacity for contraction-mediated glucose uptake.
Title: Activation of glucose transport and AMP-activated protein kinase during muscle contraction in adenylate kinase-1 knockout mice.

The following Ak1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ak1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18370	NM_021515.3 Latest version!	Mus musculus adenylate kinase 1 (Ak1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu18385	NM_001198790.1 Latest version!	Mus musculus adenylate kinase 1 (Ak1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu18385	NM_001198791.1 Latest version!	Mus musculus adenylate kinase 1 (Ak1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu18385	NM_001198792.1 Latest version!	Mus musculus adenylate kinase 1 (Ak1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu18385	XM_030246809.1 Latest version!	Mus musculus adenylate kinase 1 (Ak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu18385	XM_006497624.3 Latest version!	Mus musculus adenylate kinase 1 (Ak1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu80048	XM_017315020.1 Latest version!	Mus musculus adenylate kinase 1 (Ak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu18385	XM_006497624.2	Mus musculus adenylate kinase 1 (Ak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ak1 ( NM_021515.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067490.1 Mus musculus adenylate kinase 1 (Ak1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18370
Clone ID Related Accession (Same CDS sequence)	NM_021515.3
Accession Version	NM_021515.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Mus musculus(house mouse)
Product	adenylate kinase isoenzyme 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF950379.1 and BC014802.1. On Nov 10, 2010 this sequence version replaced NM_021515.2. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054366.1, AJ010109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCT GTGTGTCTAG TGAACCCCAA GAAGAGGGAG GCAGAAAGAC TGGAGAGAAG 
CTGAAGAAGG CCAAGATCAT CTTTGTGGTG GGCGGACCTG GCTCAGGAAA GGGCACCCAG 
TGCGAGAAGA TTGTACAGAA ATATGGCTAC ACCCACCTGT CTACTGGGGA CCTGCTTCGG 
GCAGAAGTCA GCTCTGGATC GGAGAGGGGC AAGAAGCTGT CGGCTATCAT GGAGAAGGGA 
GAGCTAGTGC CACTGGACAC GGTGCTGGAC ATGCTCCGAG ATGCTATGTT AGCCAAAGTG 
GATTCTTCCA ATGGCTTCCT GATCGACGGC TACCCGAGGG AGGTGAAACA GGGAGAAGAA 
TTTGAACAGA AGATTGGACA GCCCACACTG CTGCTGTATG TGGACGCAGG CGCCGAGACC 
ATGACCCAAC GACTCCTGAA GCGAGGGGAG ACCAGTGGCC GCGTGGATGA CAACGAGGAG 
ACCATCAAGA AGCGGCTGGA GACTTATTAC AATGCCACAG AACCTGTCAT CTCCTTCTAT 
GACAAGCGTG GCATTGTGCG CAAGGTCAAT GCCGAAGGCA CGGTGGACAC TGTCTTCTCT 
GAGGTCTGCA CCTATCTTGA CTCCCTGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067490.1
CDS	63..695
Translation	MGCCVSSEPQEEGGRKTGEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLDTVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVDDNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK

Target ORF information:

RefSeq Version	NM_021515.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenylate kinase 1 (Ak1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021515.3

ORF Insert Sequence:

ATGGGCTGCT GTGTGTCTAG TGAACCCCAA GAAGAGGGAG GCAGAAAGAC TGGAGAGAAG 
CTGAAGAAGG CCAAGATCAT CTTTGTGGTG GGCGGACCTG GCTCAGGAAA GGGCACCCAG 
TGCGAGAAGA TTGTACAGAA ATATGGCTAC ACCCACCTGT CTACTGGGGA CCTGCTTCGG 
GCAGAAGTCA GCTCTGGATC GGAGAGGGGC AAGAAGCTGT CGGCTATCAT GGAGAAGGGA 
GAGCTAGTGC CACTGGACAC GGTGCTGGAC ATGCTCCGAG ATGCTATGTT AGCCAAAGTG 
GATTCTTCCA ATGGCTTCCT GATCGACGGC TACCCGAGGG AGGTGAAACA GGGAGAAGAA 
TTTGAACAGA AGATTGGACA GCCCACACTG CTGCTGTATG TGGACGCAGG CGCCGAGACC 
ATGACCCAAC GACTCCTGAA GCGAGGGGAG ACCAGTGGCC GCGTGGATGA CAACGAGGAG 
ACCATCAAGA AGCGGCTGGA GACTTATTAC AATGCCACAG AACCTGTCAT CTCCTTCTAT 
GACAAGCGTG GCATTGTGCG CAAGGTCAAT GCCGAAGGCA CGGTGGACAC TGTCTTCTCT 
GAGGTCTGCA CCTATCTTGA CTCCCTGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ak1 ( NM_001198791.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185720.1 Mus musculus adenylate kinase 1 (Ak1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu18385
Clone ID Related Accession (Same CDS sequence)	XM_006497624.3 , XM_006497624.2 , NM_001198791.1 , NM_001198792.1 , XM_030246809.1 , NM_001198790.1
Accession Version	NM_001198791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	adenylate kinase isoenzyme 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY130582.1 and BC014802.1. Transcript Variant: This variant (3) differs at the 5' UTR and 5' coding region, compared to variant 1. The encoded protein (isoform 2) has a shorter, distinct N-terminus compared to isoform 1. Variants 2, 3, and 4 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014802.1, AK089270.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185720.1
CDS	134..718
Translation	MEEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLDTVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVDDNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK

Target ORF information:

RefSeq Version	NM_001198791.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenylate kinase 1 (Ak1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198791.1

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ak1 ( NM_001198792.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185721.1 Mus musculus adenylate kinase 1 (Ak1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu18385
Clone ID Related Accession (Same CDS sequence)	XM_006497624.3 , XM_006497624.2 , NM_001198791.1 , NM_001198792.1 , XM_030246809.1 , NM_001198790.1
Accession Version	NM_001198792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	adenylate kinase isoenzyme 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY333336.1 and BC014802.1. Transcript Variant: This variant (4) differs at the 5' UTR and 5' coding region, compared to variant 1. The encoded protein (isoform 2) has a shorter, distinct N-terminus compared to isoform 1. Variants 2, 3, and 4 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ010108.1, SRR1660811.154708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185721.1
CDS	93..677
Translation	MEEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLDTVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVDDNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK

Target ORF information:

RefSeq Version	NM_001198792.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenylate kinase 1 (Ak1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198792.1

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ak1 ( NM_001198790.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001185719.1 Mus musculus adenylate kinase 1 (Ak1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18385
Clone ID Related Accession (Same CDS sequence)	XM_006497624.3 , XM_006497624.2 , NM_001198791.1 , NM_001198792.1 , XM_030246809.1 , NM_001198790.1
Accession Version	NM_001198790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	adenylate kinase isoenzyme 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK046613.1 and BC014802.1. Transcript Variant: This variant (2) differs at the 5' UTR and 5' coding region, compared to variant 1. The encoded protein (isoform 2) has a shorter, distinct N-terminus compared to isoform 1. Variants 2, 3, and 4 encode the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK046613.1, CA465207.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185719.1
CDS	152..736
Translation	MEEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLDTVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVDDNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK

Target ORF information:

RefSeq Version	NM_001198790.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenylate kinase 1 (Ak1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198790.1

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ak1 ( XM_006497624.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497687.1 Mus musculus adenylate kinase 1 (Ak1), transcript variant X2, mRNA.

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CloneID	OMu18385
Clone ID Related Accession (Same CDS sequence)	XM_006497624.3 , XM_006497624.2 , NM_001198791.1 , NM_001198792.1 , XM_030246809.1 , NM_001198790.1
Accession Version	XM_006497624.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adenylate kinase isoenzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497624.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497687.1
CDS	48..632
Translation	MEEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLDTVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVDDNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK

Target ORF information:

RefSeq Version	XM_006497624.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenylate kinase 1 (Ak1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497624.3

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ak1 ( XM_006497624.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497687.1 Mus musculus adenylate kinase 1 (Ak1), transcript variant X1, mRNA.

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CloneID	OMu18385
Clone ID Related Accession (Same CDS sequence)	XM_006497624.3 , XM_006497624.2 , NM_001198791.1 , NM_001198792.1 , XM_030246809.1 , NM_001198790.1
Accession Version	XM_006497624.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	adenylate kinase isoenzyme 1 isoform X1
Comment

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497687.1
Translation	MEEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLDTVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVDDNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK

Target ORF information:

RefSeq Version	XM_006497624.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus adenylate kinase 1 (Ak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497624.2

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ak1 ( XM_030246809.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102669.1 Mus musculus adenylate kinase 1 (Ak1), transcript variant X1, mRNA.

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CloneID	OMu18385
Clone ID Related Accession (Same CDS sequence)	XM_006497624.3 , XM_006497624.2 , NM_001198791.1 , NM_001198792.1 , XM_030246809.1 , NM_001198790.1
Accession Version	XM_030246809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adenylate kinase isoenzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102669.1
CDS	281..865
Translation	MEEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLDTVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVDDNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK

Target ORF information:

RefSeq Version	XM_030246809.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenylate kinase 1 (Ak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246809.1

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA GGCCAAGATC ATCTTTGTGG TGGGCGGACC TGGCTCAGGA 
AAGGGCACCC AGTGCGAGAA GATTGTACAG AAATATGGCT ACACCCACCT GTCTACTGGG 
GACCTGCTTC GGGCAGAAGT CAGCTCTGGA TCGGAGAGGG GCAAGAAGCT GTCGGCTATC 
ATGGAGAAGG GAGAGCTAGT GCCACTGGAC ACGGTGCTGG ACATGCTCCG AGATGCTATG 
TTAGCCAAAG TGGATTCTTC CAATGGCTTC CTGATCGACG GCTACCCGAG GGAGGTGAAA 
CAGGGAGAAG AATTTGAACA GAAGATTGGA CAGCCCACAC TGCTGCTGTA TGTGGACGCA 
GGCGCCGAGA CCATGACCCA ACGACTCCTG AAGCGAGGGG AGACCAGTGG CCGCGTGGAT 
GACAACGAGG AGACCATCAA GAAGCGGCTG GAGACTTATT ACAATGCCAC AGAACCTGTC 
ATCTCCTTCT ATGACAAGCG TGGCATTGTG CGCAAGGTCA ATGCCGAAGG CACGGTGGAC 
ACTGTCTTCT CTGAGGTCTG CACCTATCTT GACTCCCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ak1 ( XM_017315020.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170509.1 Mus musculus adenylate kinase 1 (Ak1), transcript variant X1, mRNA.

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CloneID	OMu80048
Clone ID Related Accession (Same CDS sequence)	XM_017315020.1
Accession Version	XM_017315020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adenylate kinase isoenzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGCT TGCTTGCGAG GTTTCTTCTC TCATTGAGGA CCGTGGTGGT GTCTGCCTGG 
GGCTGTGTTG TTTTGCAGGG TGGGGTGAAG TTTCTGAATG CATGGGTGCT TACAGGCGGA 
CCTGGCTCAG GAAAGGGCAC CCAGTGCGAG AAGATTGTAC AGAAATATGG CTACACCCAC 
CTGTCTACTG GGGACCTGCT TCGGGCAGAA GTCAGCTCTG GATCGGAGAG GGGCAAGAAG 
CTGTCGGCTA TCATGGAGAA GGGAGAGCTA GTGCCACTGG ACACGGTGCT GGACATGCTC 
CGAGATGCTA TGTTAGCCAA AGTGGATTCT TCCAATGGCT TCCTGATCGA CGGCTACCCG 
AGGGAGGTGA AACAGGGAGA AGAATTTGAA CAGAAGATTG GACAGCCCAC ACTGCTGCTG 
TATGTGGACG CAGGCGCCGA GACCATGACC CAACGACTCC TGAAGCGAGG GGAGACCAGT 
GGCCGCGTGG ATGACAACGA GGAGACCATC AAGAAGCGGC TGGAGACTTA TTACAATGCC 
ACAGAACCTG TCATCTCCTT CTATGACAAG CGTGGCATTG TGCGCAAGGT CAATGCCGAA 
GGCACGGTGG ACACTGTCTT CTCTGAGGTC TGCACCTATC TTGACTCCCT GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170509.1
CDS	1781..2437
Translation	MKGLLARFLLSLRTVVVSAWGCVVLQGGVKFLNAWVLTGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLDTVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVDDNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK

Target ORF information:

RefSeq Version	XM_017315020.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenylate kinase 1 (Ak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315020.1

ORF Insert Sequence:

ATGAAAGGCT TGCTTGCGAG GTTTCTTCTC TCATTGAGGA CCGTGGTGGT GTCTGCCTGG 
GGCTGTGTTG TTTTGCAGGG TGGGGTGAAG TTTCTGAATG CATGGGTGCT TACAGGCGGA 
CCTGGCTCAG GAAAGGGCAC CCAGTGCGAG AAGATTGTAC AGAAATATGG CTACACCCAC 
CTGTCTACTG GGGACCTGCT TCGGGCAGAA GTCAGCTCTG GATCGGAGAG GGGCAAGAAG 
CTGTCGGCTA TCATGGAGAA GGGAGAGCTA GTGCCACTGG ACACGGTGCT GGACATGCTC 
CGAGATGCTA TGTTAGCCAA AGTGGATTCT TCCAATGGCT TCCTGATCGA CGGCTACCCG 
AGGGAGGTGA AACAGGGAGA AGAATTTGAA CAGAAGATTG GACAGCCCAC ACTGCTGCTG 
TATGTGGACG CAGGCGCCGA GACCATGACC CAACGACTCC TGAAGCGAGG GGAGACCAGT 
GGCCGCGTGG ATGACAACGA GGAGACCATC AAGAAGCGGC TGGAGACTTA TTACAATGCC 
ACAGAACCTG TCATCTCCTT CTATGACAAG CGTGGCATTG TGCGCAAGGT CAATGCCGAA 
GGCACGGTGG ACACTGTCTT CTCTGAGGTC TGCACCTATC TTGACTCCCT GAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Development (Cambridge, England)144(17)3177-3188(2017 09)
Rutledge EA,Benazet JD,McMahon AP

Tpbpa-Cre-mediated deletion of TFAP2C leads to deregulation of Cdkn1a, Akt1 and the ERK pathway, causing placental growth arrest.
Development (Cambridge, England)143(5)787-98(2016 Mar)
Sharma N,Kubaczka C,Kaiser S,Nettersheim D,Mughal SS,Riesenberg S,H?lzel M,Winterhager E,Schorle H

Adenine nucleotide metabolism and a role for AMP in modulating flagellar waveforms in mouse sperm.
Biology of reproduction90(6)128(2014 Jun)
Vadnais ML,Cao W,Aghajanian HK,Haig-Ladewig L,Lin AM,Al-Alao O,Gerton GL

CaMKI? regulates AMP kinase-dependent, TORC-1-independent autophagy during lipopolysaccharide-induced acute lung neutrophilic inflammation.
Journal of immunology (Baltimore, Md. : 1950)190(7)3620-8(2013 Apr)
Guo L,Stripay JL,Zhang X,Collage RD,Hulver M,Carchman EH,Howell GM,Zuckerbraun BS,Lee JS,Rosengart MR

Conditional deletion of pten leads to defects in nerve innervation and neuronal survival in inner ear development.
PloS one8(2)e55609(2013)
Kim HJ,Woo HM,Ryu J,Bok J,Kim JW,Choi SB,Park MH,Park HY,Koo SK

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Ak1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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