Aim1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aim1
Entrez Gene ID	11630
Full Name	crystallin beta-gamma domain containing 1
Synonyms	AI462491,AI463325,Aim1
General protein information	Preferred Names crystallin beta-gamma domain containing 1 Names absent in melanoma 1 protein absent in melanoma 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 B2\|10 23.14 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001368305.1	NP_001355234.1	beta/gamma crystallin domain-containing protein 1 isoform 1
XM_006512498.3	XP_006512561.1	absent in melanoma 1 protein isoform X1
NM_172393.2	NP_765981.2	beta/gamma crystallin domain-containing protein 1 isoform 3
NM_001368306.1	NP_001355235.1	beta/gamma crystallin domain-containing protein 1 isoform 2
XM_006512499.3	XP_006512562.1	absent in melanoma 1 protein isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	aim1	XP_004914608.1
Canis lupus familiaris (dog)	AIM1	XP_005627838.1
Homo sapiens (human)	AIM1	NP_001615.1
Pan troglodytes (chimpanzee)	AIM1	XP_518660.3
Rattus norvegicus (Norway rat)	Aim1	XP_006256655.1
Gallus gallus (chicken)	AIM1	XP_004940405.1
	AIM1	XP_001088619.2
Mus musculus (house mouse)	Aim1	NP_765981.2
Bos taurus (cattle)	AIM1	XP_003586516.2

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Tamplin OJ, Cox BJ, Rossant J
Developmental biology360(2)415-25(2011 Dec)

Physical and transcriptional map of the mouse Chromosome 10 proximal region syntenic to human 6q16-q21.
Chalhoub N, Benachenhou N, Vacher J
Mammalian genome : official journal of the International Mammalian Genome Society12(12)887-92(2001 Dec)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

Cloning and tissue expression of the mouse ortholog of AIM1, a betagamma-crystallin superfamily member.
Teichmann U, Ray ME, Ellison J, Graham C, Wistow G, Meltzer PS, Trent JM, Pavan WJ
Mammalian genome : official journal of the International Mammalian Genome Society9(9)715-20(1998 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Aim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03370	NM_172393.2 Latest version!	Mus musculus crystallin beta-gamma domain containing 1 (Crybg1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$951.30 $1359.00
OMu43696	NM_001368306.1 Latest version!	Mus musculus crystallin beta-gamma domain containing 1 (Crybg1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu43696	XM_006512499.3 Latest version!	Mus musculus absent in melanoma 1 (Aim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu43695	NM_001368305.1 Latest version!	Mus musculus crystallin beta-gamma domain containing 1 (Crybg1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu43695	XM_006512498.3 Latest version!	Mus musculus absent in melanoma 1 (Aim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aim1 ( NM_172393.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_765981.2 Mus musculus crystallin beta-gamma domain containing 1 (Crybg1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu03370
Clone ID Related Accession (Same CDS sequence)	NM_172393.2
Accession Version	NM_172393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-09
Organism	Mus musculus(house mouse)
Product	beta/gamma crystallin domain-containing protein 1 isoform 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC153534.2, AK041082.1 and AC158637.3. On or before Dec 30, 2007 this sequence version replaced XM_001481312.1, XM_894444.3, NM_172393.1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAAAAGA GGTCAAGCGG CCGCCGGTCT GGTCGGCGGA GGAAGTCGCA GAAATCCACC 
GACTCCCCGG GTGCGGATGC GGTGGCTCCC GAGAGTGCGG CCAAGGACGA GGCGGTGTTC 
GACGACGAGG TGGCGCCAGA CGCTGCGGCT GAGAACTGCT TGGCGGAAAG GAAAGTGAAA 
TCGGTACAAG CGGTTCCCGA CGGAGGCGCA GCCCCGGTCG CCTGCCCGGA GCCAAAGGCC 
AGCTCCTGCC CCAGAGGGCA ACCCCGAGGG GAACCGGACC GCAGCAAGCC CCTGTCGCCC 
ACCAAGCGGA AGGGTCGCAG CCGTGTCCCC GAGGCGGTGC CCAGCTCGCC CGGCAGTGGC 
TCGCGCGCTC CCGCCAAGGA GTCCCCACCC AAGAGGGCGC CCGCTGCGGC GGACAGCGGC 
GAGGAGGCGG GCCGCGTCGT CCCGCGCGAG CTCACCGTCA AAAGCAGCTC GTTGCTGCCC 
GAGATCAAGC CAGAGCACAA GAGGGGTCCT CTGCCCCACC ACTTTGATAG CCGGGGCGAC 
GGGGGTCGCA GCAGGGGAAC AGGAGCGTCC GACGCCGACG GCCTGAAGCC CAGGAACCAG 
TTCGGTGTGG GCAGGTCTAC TGTGACCACC AAAGTGACCC TCCCCGCCAG ACCCAAACAT 
GTGGAACTAA ATCTGAAAAC TCCGAAGAAT CCTGACAGTT TGGGAAATGA GCACAACTCA 
TTTAGCCAGC CAGTTCATAA GGGGAACACA GCCAACAAAA TCTCCTTATT TGAAAACAAG 
CGGGCTAACA GTAGCCCCCG CCACCCCGAC GTCCGTAACA CAAGGAACAA TCCTCCCTCT 
AGTAAGACAT TTGTTGGGAG GGCGAAGCTG AACTTAGCCA AAAAGGCCAA AGAAATGGAG 
CAACCCGAAA AGAAAGCTGT GTCAACCAGC CACCAGAATG GCCTGGTGGT CAAAGAGCCA 
TCTGCAGAAG GGAAAGGCAC AGCAGTCCCT GTCCCCAGAG AAGAGATTCG TCCAGCCACC 
AGAGGAGGGG AAGGAGAGTC CACCAAGGAT CAGGTCCTCC GCCCTGAGCC TGAGCAAAGT 
GATAAGCTGG ATGTACAAAC AGACACTGCC TATCCTTCCC ACCCAGGGCC CACTGCTCTG 
GTTCCTGTGA AGGACTCTGA GCTCTCAGGA GAGGGCCAGC CAGAGGCTGC TGACAGCAAA 
GGCCCCGTCC TTAACAACAC AAGTGATGTT GCACAGGACA TCGCTGCCAT TTGTGATGCC 
AGAGAGGCTC CCCCAAAGCC ACAGGATGCA TTTTCTGACT CACAGCCAGC TTCAGCGGCT 
GAGTCAGCTA GGGAGTCATC TCTCCCGCCT TCTGCACCCA TTTCTGTGGA CGCTCCCCAA 
GATGGCTGTG TTCAAGTTCC CATATGCAGT GTTCCCTGCA CTGCTCTAGA AGTGTCAGAA 
AACCACAATG GGTGTGTTTC GCCAGTCTCT CCTCACACTG AGAAAACTCC CATCTCCAAA 
CCTGGAGTGG GAGAAGCCAG TCCCCCTTCC ACTCCAGAGC ACAGCCCAGA AACTATGGGG 
AAAGACTGTC CCTCCAAAGT CCTCGTCCAG GTCCGGTCCT TTGTGCTTCC CGTGGAGAAT 
CCTCAGGATG TGAGCTCTCA GGTCATCTCA GAGAGCTCTG AAGTTAGAGA GGCACAGCTG 
CCAAGCTGTC ACAGTAATGA GCCTGAAGTG GTGTCTGTCA CCACTTGTGT TCCGCAGCAC 
GAGGATGCAC CAGACACATC ACCCAAGGAC ATCCATCACA GAGAAGGACA TGTGGCACAG 
TCTCGACCAC ACATCACACA GTCAGGACCT GAGAAGGCTC CGCCCATCCA GAGCAGCAGT 
GGGATGCCCC AGACAGCGGA GTTCATCCTC ACCCACTCTC CCAGCAGCAG AAGTCATGCA 
GAAGGCCCCC AAAGGCCAGG TCAAGATGCG ACCAGCCCAC TTACCCCTAC CAGGCTTTTG 
AACATTTCTG CTGGTAGTGA CAATAGTGTC TTGGATTCTT CTTCTGATAT GGAAAAATTC 
ACTGAAATTA TTAAAAAGAT GGACAGTGCA GTATGTGTGC CCCAGAAAAA GAAGAAGGCC 
CGGGTCCCAA ACTCTCCAGC CCCTCACTTC GCTATGCCTC CCATTCACGA GGACAGTTTG 
GAAAAGGTGT TTGATCCCAA CGTGTTCACC ATTGGTTTAG GGAAGAAGAA GGAAAGCCTG 
GCAGAGGTGT CACCAGCTTT GCACTTGTTG CAGAACCACG ACCCCATATC CAAACTGAGA 
GCCAAGCGTG CATCCACCGA GCAGAGCATC GTCTTCAAGT CCTTGCACAC TAACAGTAAT 
GGGAAGAGCG AGCCACAGAC CACTTCAGAA GCAAGTGACA AGGAGAACAG GGACATCAAC 
AGCGGCGGCG TTAAGAGGTC ACGGCTAGAA AACAGCACGC TTTTCTCAAG CATGCTTTCT 
TCTCTGCCAC AAGACAAAGT CTTTTCTCCC TCCGTGACGT CAGTCAACAC AATGAGCACA 
TCTTTTAGCA CTTCTCAGAA CAGCTCCGTA TCTCAGTCAT CAGTGGTACA GCCCAGGAAG 
GAGGGTGGCC TGCCCTGTGG CTCAGACAAA GAACAGCCAC ACCTTGCACC CCACAACTCC 
TTAAAGGTCT TCAATTTCAA TTCATCAAAT ACATCTCGTC TGGGTTCCAG CCACATGGAA 
AAGTACCCAC AAAAAGAGGG AAGCAAGGAA GATGTGAATT CTCAGGGCAA CCTACAATTG 
CCAGAAAACA ACCTTTCAGA CTTACCCAAA CTGAAGAACA GCGATGGCGT GGAAGGTATT 
CTGAAGTCAA ACCTGCCGAA TGTTGGGAGC GTGGACGCGG ACTTCCTGGG CCTTTTCAAA 
GCAAGCCGGT ATGACCCAGG CCATTCCTTT TCTGGAATGT CCTTATCAGA CTCTATGACT 
CTTAGAGGAA GTATGCACAG CAAACTCAAC CCCCGGCCTG GCAAGGTAGT GATATTCAGT 
GAGCCCGACG TCTCTGAGGA GTGCATTGAG GTGTTTGGGG ACATCCAGGA CTGCAGCTCC 
TGGCGCCTCT CTCCAGTGAT AGTAGTGAAA GTTGTCCGAG GATGTTGGAT TTTGTATGAG 
AAAGCCAATT TTGAAGGGCA TTCTCTTGCT TTAGAAGAAG GTGAACTGGA ACTCTCTAGT 
ATCTGGGGAA CAGAGGAAAT GTTGGATGAA GAAGCAGAGT CTGATAAACC TGTAGTGATT 
GGTTCCATCA GACACGTGGT TCAGGATTAC AGAATTAGTC AGATCGACTT GTTTACTGAG 
CCTGAAGGGT TAGGACTCCT GAACTCGTAC TTTGATGACA CTGAGGAAAT GCAGGGCTTC 
CCTGTGATGC AGAAGACGTG TTCCATCAAA GTGCACTGGG GCACATGGCT GATCTATGAA 
GAGCCTGGAT TCCAAGGTGT CCCCTTCATT CTGGAGCCCG GAGAATACCC TGACCTATCC 
TTCTGGGATA CCGAGGAAGC GTACATCGGA TCCATGCGGC CTCTGAAAAT GGGTGGCCGT 
AAAGTTGAAT TCCCTACAGA TCCAAAGGTA GTTATTTATG AAAAGCCTTC CTTTGAAGGA 
AGGTGTATGG AGCTAGAAAC AGAAATGAGT AGTTTTATCA TGGAAGGAGG TGAGACAGAA 
GAAACAACTG GAAACAACAA CTTGCCCTTT ACATCCGTGG GGTCTATGAA AGTCCTAAGA 
GGCGTCTGGG TTGCTTATGA GAAGTGTGGC TTCACTGGCC ACCAGTATTT GCTGGAGGAG 
GGAGAATACA GGGACTGGAA CGCCTGGGGA GGCTACAATG GAGAGCTCCA GTCTCTGCGG 
CCTATATTAA GTGATTTTTC AAATGCGCAC ATGATAATGT ACAGTGAGAA GAACTTTGGC 
TCCAAAGGTT CCAGTATTGA TGTGTTGGGA ATTGTTGCCA ATTTGAAGGA GACCGGCTAC 
GGAGTGAAGA CACAGTCAAT CAATGTACTG AGTGGCGTGT GGGTAGCCTA TGAAAACCCG 
GACTTCACGG GGGAGCAGTA TGTCCTGGAT AAAGGCTTCT ACACCAGTTT TGAGGATTGG 
GGAGGCAAAA ATTGTAAGAT CTCTTCCGTC CAACCCATAT GCCTGGATTC TTTCACTGGC 
CCCAGGAGAC GAAATCAGAT TCACTTGTTT TCAGAACCAC AGTTCCAAGG TCAAAGTCAA 
AGTTTTGAAG AAACAATAGA TCAGATTGAC GACTCCTTTC AGACCAAGTC TTGCAGAGTT 
TTGGGAGGCA GCTGGGTTGC TTATGATGGA GAAAATTTCT CTGGCAATCA GTATGTACTG 
GAAGAAGGCC ACTATCCTTG TCTCTCGGCA ATGGGATGCC TGCCTGGAGC AACCCTGAAG 
TCTCTTCGCT TTATAGATTT TGAATTTTCC GAACCAACAA TTATTCTTTT CGAAAGAGAA 
AACTTCAAAG GAAAAAAGAT AGAACTGAAC GCAGAAACAG TCAATCTCCG ATCCTTGGGG 
TTCAACACTC AGATCCGCTC TGTGCGGGTC GTTGGTGGCA TATGGGTTAC CTATGAATAT 
GGCAATTACA GAGGCCGGCA GTTCCTGCTG TCTCCTGCAG AAGTACCAAA CTGGTATGAG 
TTCAGTGCCT GTCGCCAAAT AGGCTCCCTC CGGCCCTTTG CTCAGAAACG AATTTACTTC 
AGACTTCGAA ACAAAGCAAC AGGACTATTT ATGTCAACCA ACGGGAACTT GGAAGACCTG 
AAGCTTCTCC GAGTCCAGGT CATGGAAGAT GTTGGTGCTG ATGATCAGAT CTGGATCTAT 
CAAGAAGGGT GCATCAAATG CAGGATAGCA GAAGACTGCT GCCTGACGAT CGTGGGCAGC 
CTCGTCACGT CTGGCTCCAA ACTAGGACTG GCCCTCGATC AAAATGTCGA CAGTCAGTTC 
TGGTGCATGA AGTCCGACGG CAGGATCTAC AGCAAGATGA AACCAAATTT AGTGTTGGAT 
GTGAAAGGGG GTGCGCAGTA TGATCAAAAT CACATCATCC TCAACACAGT GAGCACAGAG 
AAGTTAACAC AAGTGTGGGA AGCCATGGTC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_765981.2
CDS	779..5854
Translation	MEKRSSGRRSGRRRKSQKSTDSPGADAVAPESAAKDEAVFDDEVAPDAAAENCLAERKVKSVQAVPDGGAAPVACPEPKASSCPRGQPRGEPDRSKPLSPTKRKGRSRVPEAVPSSPGSGSRAPAKESPPKRAPAAADSGEEAGRVVPRELTVKSSSLLPEIKPEHKRGPLPHHFDSRGDGGRSRGTGASDADGLKPRNQFGVGRSTVTTKVTLPARPKHVELNLKTPKNPDSLGNEHNSFSQPVHKGNTANKISLFENKRANSSPRHPDVRNTRNNPPSSKTFVGRAKLNLAKKAKEMEQPEKKAVSTSHQNGLVVKEPSAEGKGTAVPVPREEIRPATRGGEGESTKDQVLRPEPEQSDKLDVQTDTAYPSHPGPTALVPVKDSELSGEGQPEAADSKGPVLNNTSDVAQDIAAICDAREAPPKPQDAFSDSQPASAAESARESSLPPSAPISVDAPQDGCVQVPICSVPCTALEVSENHNGCVSPVSPHTEKTPISKPGVGEASPPSTPEHSPETMGKDCPSKVLVQVRSFVLPVENPQDVSSQVISESSEVREAQLPSCHSNEPEVVSVTTCVPQHEDAPDTSPKDIHHREGHVAQSRPHITQSGPEKAPPIQSSSGMPQTAEFILTHSPSSRSHAEGPQRPGQDATSPLTPTRLLNISAGSDNSVLDSSSDMEKFTEIIKKMDSAVCVPQKKKKARVPNSPAPHFAMPPIHEDSLEKVFDPNVFTIGLGKKKESLAEVSPALHLLQNHDPISKLRAKRASTEQSIVFKSLHTNSNGKSEPQTTSEASDKENRDINSGGVKRSRLENSTLFSSMLSSLPQDKVFSPSVTSVNTMSTSFSTSQNSSVSQSSVVQPRKEGGLPCGSDKEQPHLAPHNSLKVFNFNSSNTSRLGSSHMEKYPQKEGSKEDVNSQGNLQLPENNLSDLPKLKNSDGVEGILKSNLPNVGSVDADFLGLFKASRYDPGHSFSGMSLSDSMTLRGSMHSKLNPRPGKVVIFSEPDVSEECIEVFGDIQDCSSWRLSPVIVVKVVRGCWILYEKANFEGHSLALEEGELELSSIWGTEEMLDEEAESDKPVVIGSIRHVVQDYRISQIDLFTEPEGLGLLNSYFDDTEEMQGFPVMQKTCSIKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVIYEKPSFEGRCMELETEMSSFIMEGGETEETTGNNNLPFTSVGSMKVLRGVWVAYEKCGFTGHQYLLEEGEYRDWNAWGGYNGELQSLRPILSDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYVLDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGQSQSFEETIDQIDDSFQTKSCRVLGGSWVAYDGENFSGNQYVLEEGHYPCLSAMGCLPGATLKSLRFIDFEFSEPTIILFERENFKGKKIELNAETVNLRSLGFNTQIRSVRVVGGIWVTYEYGNYRGRQFLLSPAEVPNWYEFSACRQIGSLRPFAQKRIYFRLRNKATGLFMSTNGNLEDLKLLRVQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNVDSQFWCMKSDGRIYSKMKPNLVLDVKGGAQYDQNHIILNTVSTEKLTQVWEAMVL

Target ORF information:

RefSeq Version	NM_172393.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin beta-gamma domain containing 1 (Crybg1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172393.2

ORF Insert Sequence:

ATGGAAAAGA GGTCAAGCGG CCGCCGGTCT GGTCGGCGGA GGAAGTCGCA GAAATCCACC 
GACTCCCCGG GTGCGGATGC GGTGGCTCCC GAGAGTGCGG CCAAGGACGA GGCGGTGTTC 
GACGACGAGG TGGCGCCAGA CGCTGCGGCT GAGAACTGCT TGGCGGAAAG GAAAGTGAAA 
TCGGTACAAG CGGTTCCCGA CGGAGGCGCA GCCCCGGTCG CCTGCCCGGA GCCAAAGGCC 
AGCTCCTGCC CCAGAGGGCA ACCCCGAGGG GAACCGGACC GCAGCAAGCC CCTGTCGCCC 
ACCAAGCGGA AGGGTCGCAG CCGTGTCCCC GAGGCGGTGC CCAGCTCGCC CGGCAGTGGC 
TCGCGCGCTC CCGCCAAGGA GTCCCCACCC AAGAGGGCGC CCGCTGCGGC GGACAGCGGC 
GAGGAGGCGG GCCGCGTCGT CCCGCGCGAG CTCACCGTCA AAAGCAGCTC GTTGCTGCCC 
GAGATCAAGC CAGAGCACAA GAGGGGTCCT CTGCCCCACC ACTTTGATAG CCGGGGCGAC 
GGGGGTCGCA GCAGGGGAAC AGGAGCGTCC GACGCCGACG GCCTGAAGCC CAGGAACCAG 
TTCGGTGTGG GCAGGTCTAC TGTGACCACC AAAGTGACCC TCCCCGCCAG ACCCAAACAT 
GTGGAACTAA ATCTGAAAAC TCCGAAGAAT CCTGACAGTT TGGGAAATGA GCACAACTCA 
TTTAGCCAGC CAGTTCATAA GGGGAACACA GCCAACAAAA TCTCCTTATT TGAAAACAAG 
CGGGCTAACA GTAGCCCCCG CCACCCCGAC GTCCGTAACA CAAGGAACAA TCCTCCCTCT 
AGTAAGACAT TTGTTGGGAG GGCGAAGCTG AACTTAGCCA AAAAGGCCAA AGAAATGGAG 
CAACCCGAAA AGAAAGCTGT GTCAACCAGC CACCAGAATG GCCTGGTGGT CAAAGAGCCA 
TCTGCAGAAG GGAAAGGCAC AGCAGTCCCT GTCCCCAGAG AAGAGATTCG TCCAGCCACC 
AGAGGAGGGG AAGGAGAGTC CACCAAGGAT CAGGTCCTCC GCCCTGAGCC TGAGCAAAGT 
GATAAGCTGG ATGTACAAAC AGACACTGCC TATCCTTCCC ACCCAGGGCC CACTGCTCTG 
GTTCCTGTGA AGGACTCTGA GCTCTCAGGA GAGGGCCAGC CAGAGGCTGC TGACAGCAAA 
GGCCCCGTCC TTAACAACAC AAGTGATGTT GCACAGGACA TCGCTGCCAT TTGTGATGCC 
AGAGAGGCTC CCCCAAAGCC ACAGGATGCA TTTTCTGACT CACAGCCAGC TTCAGCGGCT 
GAGTCAGCTA GGGAGTCATC TCTCCCGCCT TCTGCACCCA TTTCTGTGGA CGCTCCCCAA 
GATGGCTGTG TTCAAGTTCC CATATGCAGT GTTCCCTGCA CTGCTCTAGA AGTGTCAGAA 
AACCACAATG GGTGTGTTTC GCCAGTCTCT CCTCACACTG AGAAAACTCC CATCTCCAAA 
CCTGGAGTGG GAGAAGCCAG TCCCCCTTCC ACTCCAGAGC ACAGCCCAGA AACTATGGGG 
AAAGACTGTC CCTCCAAAGT CCTCGTCCAG GTCCGGTCCT TTGTGCTTCC CGTGGAGAAT 
CCTCAGGATG TGAGCTCTCA GGTCATCTCA GAGAGCTCTG AAGTTAGAGA GGCACAGCTG 
CCAAGCTGTC ACAGTAATGA GCCTGAAGTG GTGTCTGTCA CCACTTGTGT TCCGCAGCAC 
GAGGATGCAC CAGACACATC ACCCAAGGAC ATCCATCACA GAGAAGGACA TGTGGCACAG 
TCTCGACCAC ACATCACACA GTCAGGACCT GAGAAGGCTC CGCCCATCCA GAGCAGCAGT 
GGGATGCCCC AGACAGCGGA GTTCATCCTC ACCCACTCTC CCAGCAGCAG AAGTCATGCA 
GAAGGCCCCC AAAGGCCAGG TCAAGATGCG ACCAGCCCAC TTACCCCTAC CAGGCTTTTG 
AACATTTCTG CTGGTAGTGA CAATAGTGTC TTGGATTCTT CTTCTGATAT GGAAAAATTC 
ACTGAAATTA TTAAAAAGAT GGACAGTGCA GTATGTGTGC CCCAGAAAAA GAAGAAGGCC 
CGGGTCCCAA ACTCTCCAGC CCCTCACTTC GCTATGCCTC CCATTCACGA GGACAGTTTG 
GAAAAGGTGT TTGATCCCAA CGTGTTCACC ATTGGTTTAG GGAAGAAGAA GGAAAGCCTG 
GCAGAGGTGT CACCAGCTTT GCACTTGTTG CAGAACCACG ACCCCATATC CAAACTGAGA 
GCCAAGCGTG CATCCACCGA GCAGAGCATC GTCTTCAAGT CCTTGCACAC TAACAGTAAT 
GGGAAGAGCG AGCCACAGAC CACTTCAGAA GCAAGTGACA AGGAGAACAG GGACATCAAC 
AGCGGCGGCG TTAAGAGGTC ACGGCTAGAA AACAGCACGC TTTTCTCAAG CATGCTTTCT 
TCTCTGCCAC AAGACAAAGT CTTTTCTCCC TCCGTGACGT CAGTCAACAC AATGAGCACA 
TCTTTTAGCA CTTCTCAGAA CAGCTCCGTA TCTCAGTCAT CAGTGGTACA GCCCAGGAAG 
GAGGGTGGCC TGCCCTGTGG CTCAGACAAA GAACAGCCAC ACCTTGCACC CCACAACTCC 
TTAAAGGTCT TCAATTTCAA TTCATCAAAT ACATCTCGTC TGGGTTCCAG CCACATGGAA 
AAGTACCCAC AAAAAGAGGG AAGCAAGGAA GATGTGAATT CTCAGGGCAA CCTACAATTG 
CCAGAAAACA ACCTTTCAGA CTTACCCAAA CTGAAGAACA GCGATGGCGT GGAAGGTATT 
CTGAAGTCAA ACCTGCCGAA TGTTGGGAGC GTGGACGCGG ACTTCCTGGG CCTTTTCAAA 
GCAAGCCGGT ATGACCCAGG CCATTCCTTT TCTGGAATGT CCTTATCAGA CTCTATGACT 
CTTAGAGGAA GTATGCACAG CAAACTCAAC CCCCGGCCTG GCAAGGTAGT GATATTCAGT 
GAGCCCGACG TCTCTGAGGA GTGCATTGAG GTGTTTGGGG ACATCCAGGA CTGCAGCTCC 
TGGCGCCTCT CTCCAGTGAT AGTAGTGAAA GTTGTCCGAG GATGTTGGAT TTTGTATGAG 
AAAGCCAATT TTGAAGGGCA TTCTCTTGCT TTAGAAGAAG GTGAACTGGA ACTCTCTAGT 
ATCTGGGGAA CAGAGGAAAT GTTGGATGAA GAAGCAGAGT CTGATAAACC TGTAGTGATT 
GGTTCCATCA GACACGTGGT TCAGGATTAC AGAATTAGTC AGATCGACTT GTTTACTGAG 
CCTGAAGGGT TAGGACTCCT GAACTCGTAC TTTGATGACA CTGAGGAAAT GCAGGGCTTC 
CCTGTGATGC AGAAGACGTG TTCCATCAAA GTGCACTGGG GCACATGGCT GATCTATGAA 
GAGCCTGGAT TCCAAGGTGT CCCCTTCATT CTGGAGCCCG GAGAATACCC TGACCTATCC 
TTCTGGGATA CCGAGGAAGC GTACATCGGA TCCATGCGGC CTCTGAAAAT GGGTGGCCGT 
AAAGTTGAAT TCCCTACAGA TCCAAAGGTA GTTATTTATG AAAAGCCTTC CTTTGAAGGA 
AGGTGTATGG AGCTAGAAAC AGAAATGAGT AGTTTTATCA TGGAAGGAGG TGAGACAGAA 
GAAACAACTG GAAACAACAA CTTGCCCTTT ACATCCGTGG GGTCTATGAA AGTCCTAAGA 
GGCGTCTGGG TTGCTTATGA GAAGTGTGGC TTCACTGGCC ACCAGTATTT GCTGGAGGAG 
GGAGAATACA GGGACTGGAA CGCCTGGGGA GGCTACAATG GAGAGCTCCA GTCTCTGCGG 
CCTATATTAA GTGATTTTTC AAATGCGCAC ATGATAATGT ACAGTGAGAA GAACTTTGGC 
TCCAAAGGTT CCAGTATTGA TGTGTTGGGA ATTGTTGCCA ATTTGAAGGA GACCGGCTAC 
GGAGTGAAGA CACAGTCAAT CAATGTACTG AGTGGCGTGT GGGTAGCCTA TGAAAACCCG 
GACTTCACGG GGGAGCAGTA TGTCCTGGAT AAAGGCTTCT ACACCAGTTT TGAGGATTGG 
GGAGGCAAAA ATTGTAAGAT CTCTTCCGTC CAACCCATAT GCCTGGATTC TTTCACTGGC 
CCCAGGAGAC GAAATCAGAT TCACTTGTTT TCAGAACCAC AGTTCCAAGG TCAAAGTCAA 
AGTTTTGAAG AAACAATAGA TCAGATTGAC GACTCCTTTC AGACCAAGTC TTGCAGAGTT 
TTGGGAGGCA GCTGGGTTGC TTATGATGGA GAAAATTTCT CTGGCAATCA GTATGTACTG 
GAAGAAGGCC ACTATCCTTG TCTCTCGGCA ATGGGATGCC TGCCTGGAGC AACCCTGAAG 
TCTCTTCGCT TTATAGATTT TGAATTTTCC GAACCAACAA TTATTCTTTT CGAAAGAGAA 
AACTTCAAAG GAAAAAAGAT AGAACTGAAC GCAGAAACAG TCAATCTCCG ATCCTTGGGG 
TTCAACACTC AGATCCGCTC TGTGCGGGTC GTTGGTGGCA TATGGGTTAC CTATGAATAT 
GGCAATTACA GAGGCCGGCA GTTCCTGCTG TCTCCTGCAG AAGTACCAAA CTGGTATGAG 
TTCAGTGCCT GTCGCCAAAT AGGCTCCCTC CGGCCCTTTG CTCAGAAACG AATTTACTTC 
AGACTTCGAA ACAAAGCAAC AGGACTATTT ATGTCAACCA ACGGGAACTT GGAAGACCTG 
AAGCTTCTCC GAGTCCAGGT CATGGAAGAT GTTGGTGCTG ATGATCAGAT CTGGATCTAT 
CAAGAAGGGT GCATCAAATG CAGGATAGCA GAAGACTGCT GCCTGACGAT CGTGGGCAGC 
CTCGTCACGT CTGGCTCCAA ACTAGGACTG GCCCTCGATC AAAATGTCGA CAGTCAGTTC 
TGGTGCATGA AGTCCGACGG CAGGATCTAC AGCAAGATGA AACCAAATTT AGTGTTGGAT 
GTGAAAGGGG GTGCGCAGTA TGATCAAAAT CACATCATCC TCAACACAGT GAGCACAGAG 
AAGTTAACAC AAGTGTGGGA AGCCATGGTC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aim1 ( NM_001368306.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355235.1 Mus musculus crystallin beta-gamma domain containing 1 (Crybg1), transcript variant 2, mRNA.

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CloneID	OMu43696
Clone ID Related Accession (Same CDS sequence)	NM_001368306.1 , XM_006512499.3
Accession Version	NM_001368306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-09
Organism	Mus musculus(house mouse)
Product	beta/gamma crystallin domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153534.2 and AC158637.3. On Feb 5, 2019 this sequence version replaced XM_006512499.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGTCCAGAG CACAACCACC AGAAGACACC AGAAGGAAGC CAGTGTTGGA AAAACTTGGC 
ACTCTTTTTA CGGCTGGAAG GAGAAGGAAC ACCAGGAACG GATTAGAGAG TCCCACCAAC 
TCAAATAGCA AATCGGCCTC TGCTAAAGTC CCAAAAACAG GGAGTGAGAA AACTAAGGCT 
CAGGACAACC AACTAAAGGC AACCGACGCT GGCGAGGCGG GCCCCAGGCA GGAGGCTGAG 
GGCCAGCCCG TTGAGAGCTG TGTTCAGGCA ACCCCCTGCG ACGCCGAACA GTCACCTTGC 
TCCAGCATCA ATGTAGGGGT TGTGCAGCAG TGCCACAGCA ATGATTCAGC CCAATTAGAA 
CCTCTACAGG CAGAGGGAGA GACTTTCCCA GATGCCACCA CCGCTGCCAA AGAGCTGCTG 
CATTCCTCAC CAGGGAATCG CTCCAGGCAG GAGAGCGCAG AGACGCCCGC CCGCAGTCCG 
GGGGAGGAAG CGTTGCCTGG CGCTGGCCTC CAACCAGAGA CAGCGAGAGG TGTGTCGGGG 
TCGCCGCCAG AGGAGCACCT CGTGGGAGGA GTAGGAGAGG CCCCCGACGG AGCCCCCGGC 
GTGGGTGCCG AAGCGGGCTC TCTGGGGGCC CCGGGGGACG CGGTTCCTCC AGGTGAGCCT 
CCCGCCCAGG ACGCAGAGAC CAGCCGCACA GCCGGGTCGG TCGACCGCGC GCATCCCACC 
AAAGTGCTAA CGTTGGACAT CTACTTGAGC AAAACTGAGG TGGCTCAGGT GGACGAGCCG 
GCCTCGATTG CTCCGGGGAC AGACGACTGC GAGGATTCGG ACGACATGGA AAAGAGGTCA 
AGCGGCCGCC GGTCTGGTCG GCGGAGGAAG TCGCAGAAAT CCACCGACTC CCCGGGTGCG 
GATGCGGTGG CTCCCGAGAG TGCGGCCAAG GACGAGGCGG TGTTCGACGA CGAGGTGGCG 
CCAGACGCTG CGGCTGAGAA CTGCTTGGCG GAAAGGAAAG TGAAATCGGT ACAAGCGGTT 
CCCGACGGAG GCGCAGCCCC GGTCGCCTGC CCGGAGCCAA AGGCCAGCTC CTGCCCCAGA 
GGGCAACCCC GAGGGGAACC GGACCGCAGC AAGCCCCTGT CGCCCACCAA GCGGAAGGGT 
CGCAGCCGTG TCCCCGAGGC GGTGCCCAGC TCGCCCGGCA GTGGCTCGCG CGCTCCCGCC 
AAGGAGTCCC CACCCAAGAG GGCGCCCGCT GCGGCGGACA GCGGCGAGGA GGCGGGCCGC 
GTCGTCCCGC GCGAGCTCAC CGTCAAAAGC AGCTCGTTGC TGCCCGAGAT CAAGCCAGAG 
CACAAGAGGG GTCCTCTGCC CCACCACTTT GATAGCCGGG GCGACGGGGG TCGCAGCAGG 
GGAACAGGAG CGTCCGACGC CGACGGCCTG AAGCCCAGGA ACCAGTTCGG TGTGGGCAGG 
TCTACTGTGA CCACCAAAGT GACCCTCCCC GCCAGACCCA AACATGTGGA ACTAAATCTG 
AAAACTCCGA AGAATCCTGA CAGTTTGGGA AATGAGCACA ACTCATTTAG CCAGCCAGTT 
CATAAGGGGA ACACAGCCAA CAAAATCTCC TTATTTGAAA ACAAGCGGGC TAACAGTAGC 
CCCCGCCACC CCGACGTCCG TAACACAAGG AACAATCCTC CCTCTAGTAA GACATTTGTT 
GGGAGGGCGA AGCTGAACTT AGCCAAAAAG GCCAAAGAAA TGGAGCAACC CGAAAAGAAA 
GCTGTGTCAA CCAGCCACCA GAATGGCCTG GTGGTCAAAG AGCCATCTGC AGAAGGGAAA 
GGCACAGCAG TCCCTGTCCC CAGAGAAGAG ATTCGTCCAG CCACCAGAGG AGGGGAAGGA 
GAGTCCACCA AGGATCAGGT CCTCCGCCCT GAGCCTGAGC AAAGTGATAA GCTGGATGTA 
CAAACAGACA CTGCCTATCC TTCCCACCCA GGGCCCACTG CTCTGGTTCC TGTGAAGGAC 
TCTGAGCTCT CAGGAGAGGG CCAGCCAGAG GCTGCTGACA GCAAAGGCCC CGTCCTTAAC 
AACACAAGTG ATGTTGCACA GGACATCGCT GCCATTTGTG ATGCCAGAGA GGCTCCCCCA 
AAGCCACAGG ATGCATTTTC TGACTCACAG CCAGCTTCAG CGGCTGAGTC AGCTAGGGAG 
TCATCTCTCC CGCCTTCTGC ACCCATTTCT GTGGACGCTC CCCAAGATGG CTGTGTTCAA 
GTTCCCATAT GCAGTGTTCC CTGCACTGCT CTAGAAGTGT CAGAAAACCA CAATGGGTGT 
GTTTCGCCAG TCTCTCCTCA CACTGAGAAA ACTCCCATCT CCAAACCTGG AGTGGGAGAA 
GCCAGTCCCC CTTCCACTCC AGAGCACAGC CCAGAAACTA TGGGGAAAGA CTGTCCCTCC 
AAAGTCCTCG TCCAGGTCCG GTCCTTTGTG CTTCCCGTGG AGAATCCTCA GGATGTGAGC 
TCTCAGGTCA TCTCAGAGAG CTCTGAAGTT AGAGAGGCAC AGCTGCCAAG CTGTCACAGT 
AATGAGCCTG AAGTGGTGTC TGTCACCACT TGTGTTCCGC AGCACGAGGA TGCACCAGAC 
ACATCACCCA AGGACATCCA TCACAGAGAA GGACATGTGG CACAGTCTCG ACCACACATC 
ACACAGTCAG GACCTGAGAA GGCTCCGCCC ATCCAGAGCA GCAGTGGGAT GCCCCAGACA 
GCGGAGTTCA TCCTCACCCA CTCTCCCAGC AGCAGAAGTC ATGCAGAAGG CCCCCAAAGG 
CCAGGTCAAG ATGCGACCAG CCCACTTACC CCTACCAGGC TTTTGAACAT TTCTGCTGGT 
AGTGACAATA GTGTCTTGGA TTCTTCTTCT GATATGGAAA AATTCACTGA AATTATTAAA 
AAGATGGACA GTGCAGTATG TGTGCCCCAG AAAAAGAAGA AGGCCCGGGT CCCAAACTCT 
CCAGCCCCTC ACTTCGCTAT GCCTCCCATT CACGAGGACA GTTTGGAAAA GGTGTTTGAT 
CCCAACGTGT TCACCATTGG TTTAGGGAAG AAGAAGGAAA GCCTGGCAGA GGTGTCACCA 
GCTTTGCACT TGTTGCAGAA CCACGACCCC ATATCCAAAC TGAGAGCCAA GCGTGCATCC 
ACCGAGCAGA GCATCGTCTT CAAGTCCTTG CACACTAACA GTAATGGGAA GAGCGAGCCA 
CAGACCACTT CAGAAGCAAG TGACAAGGAG AACAGGGACA TCAACAGCGG CGGCGTTAAG 
AGGTCACGGC TAGAAAACAG CACGCTTTTC TCAAGCATGC TTTCTTCTCT GCCACAAGAC 
AAAGTCTTTT CTCCCTCCGT GACGTCAGTC AACACAATGA GCACATCTTT TAGCACTTCT 
CAGAACAGCT CCGTATCTCA GTCATCAGTG GTACAGCCCA GGAAGGAGGG TGGCCTGCCC 
TGTGGCTCAG ACAAAGAACA GCCACACCTT GCACCCCACA ACTCCTTAAA GGTCTTCAAT 
TTCAATTCAT CAAATACATC TCGTCTGGGT TCCAGCCACA TGGAAAAGTA CCCACAAAAA 
GAGGGAAGCA AGGAAGATGT GAATTCTCAG GGCAACCTAC AATTGCCAGA AAACAACCTT 
TCAGACTTAC CCAAACTGAA GAACAGCGAT GGCGTGGAAG GTATTCTGAA GTCAAACCTG 
CCGAATGTTG GGAGCGTGGA CGCGGACTTC CTGGGCCTTT TCAAAGCAAG CCGGTATGAC 
CCAGGCCATT CCTTTTCTGG AATGTCCTTA TCAGACTCTA TGACTCTTAG AGGAAGTATG 
CACAGCAAAC TCAACCCCCG GCCTGGCAAG GTAGTGATAT TCAGTGAGCC CGACGTCTCT 
GAGGAGTGCA TTGAGGTGTT TGGGGACATC CAGGACTGCA GCTCCTGGCG CCTCTCTCCA 
GTGATAGTAG TGAAAGTTGT CCGAGGATGT TGGATTTTGT ATGAGAAAGC CAATTTTGAA 
GGGCATTCTC TTGCTTTAGA AGAAGGTGAA CTGGAACTCT CTAGTATCTG GGGAACAGAG 
GAAATGTTGG ATGAAGAAGC AGAGTCTGAT AAACCTGTAG TGATTGGTTC CATCAGACAC 
GTGGTTCAGG ATTACAGAAT TAGTCAGATC GACTTGTTTA CTGAGCCTGA AGGGTTAGGA 
CTCCTGAACT CGTACTTTGA TGACACTGAG GAAATGCAGG GCTTCCCTGT GATGCAGAAG 
ACGTGTTCCA TCAAAGTGCA CTGGGGCACA TGGCTGATCT ATGAAGAGCC TGGATTCCAA 
GGTGTCCCCT TCATTCTGGA GCCCGGAGAA TACCCTGACC TATCCTTCTG GGATACCGAG 
GAAGCGTACA TCGGATCCAT GCGGCCTCTG AAAATGGGTG GCCGTAAAGT TGAATTCCCT 
ACAGATCCAA AGGTAGTTAT TTATGAAAAG CCTTCCTTTG AAGGAAGGTG TATGGAGCTA 
GAAACAGAAA TGAGTAGTTT TATCATGGAA GGAGGTGAGA CAGAAGAAAC AACTGGAAAC 
AACAACTTGC CCTTTACATC CGTGGGGTCT ATGAAAGTCC TAAGAGGCGT CTGGGTTGCT 
TATGAGAAGT GTGGCTTCAC TGGCCACCAG TATTTGCTGG AGGAGGGAGA ATACAGGGAC 
TGGAACGCCT GGGGAGGCTA CAATGGAGAG CTCCAGTCTC TGCGGCCTAT ATTAAGTGAT 
TTTTCAAATG CGCACATGAT AATGTACAGT GAGAAGAACT TTGGCTCCAA AGGTTCCAGT 
ATTGATGTGT TGGGAATTGT TGCCAATTTG AAGGAGACCG GCTACGGAGT GAAGACACAG 
TCAATCAATG TACTGAGTGG CGTGTGGGTA GCCTATGAAA ACCCGGACTT CACGGGGGAG 
CAGTATGTCC TGGATAAAGG CTTCTACACC AGTTTTGAGG ATTGGGGAGG CAAAAATTGT 
AAGATCTCTT CCGTCCAACC CATATGCCTG GATTCTTTCA CTGGCCCCAG GAGACGAAAT 
CAGATTCACT TGTTTTCAGA ACCACAGTTC CAAGGTCAAA GTCAAAGTTT TGAAGAAACA 
ATAGATCAGA TTGACGACTC CTTTCAGACC AAGTCTTGCA GAGTTTTGGG AGGCAGCTGG 
GTTGCTTATG ATGGAGAAAA TTTCTCTGGC AATCAGTATG TACTGGAAGA AGGCCACTAT 
CCTTGTCTCT CGGCAATGGG ATGCCTGCCT GGAGCAACCC TGAAGTCTCT TCGCTTTATA 
GATTTTGAAT TTTCCGAACC AACAATTATT CTTTTCGAAA GAGAAAACTT CAAAGGAAAA 
AAGATAGAAC TGAACGCAGA AACAGTCAAT CTCCGATCCT TGGGGTTCAA CACTCAGATC 
CGCTCTGTGC GGGTCGTTGG TGGCATATGG GTTACCTATG AATATGGCAA TTACAGAGGC 
CGGCAGTTCC TGCTGTCTCC TGCAGAAGTA CCAAACTGGT ATGAGTTCAG TGCCTGTCGC 
CAAATAGGCT CCCTCCGGCC CTTTGCTCAG AAACGAATTT ACTTCAGACT TCGAAACAAA 
GCAACAGGAC TATTTATGTC AACCAACGGG AACTTGGAAG ACCTGAAGCT TCTCCGAGTC 
CAGGTCATGG AAGATGTTGG TGCTGATGAT CAGATCTGGA TCTATCAAGA AGGGTGCATC 
AAATGCAGGA TAGCAGAAGA CTGCTGCCTG ACGATCGTGG GCAGCCTCGT CACGTCTGGC 
TCCAAACTAG GACTGGCCCT CGATCAAAAT GTCGACAGTC AGTTCTGGTG CATGAAGTCC 
GACGGCAGGA TCTACAGCAA GATGAAACCA AATTTAGTGT TGGATGTGAA AGGGGGTGCG 
CAGTATGATC AAAATCACAT CATCCTCAAC ACAGTGAGCA CAGAGAAGTT AACACAAGTG 
TGGGAAGCCA TGGTCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355235.1
CDS	182..6082
Translation	MSRAQPPEDTRRKPVLEKLGTLFTAGRRRNTRNGLESPTNSNSKSASAKVPKTGSEKTKAQDNQLKATDAGEAGPRQEAEGQPVESCVQATPCDAEQSPCSSINVGVVQQCHSNDSAQLEPLQAEGETFPDATTAAKELLHSSPGNRSRQESAETPARSPGEEALPGAGLQPETARGVSGSPPEEHLVGGVGEAPDGAPGVGAEAGSLGAPGDAVPPGEPPAQDAETSRTAGSVDRAHPTKVLTLDIYLSKTEVAQVDEPASIAPGTDDCEDSDDMEKRSSGRRSGRRRKSQKSTDSPGADAVAPESAAKDEAVFDDEVAPDAAAENCLAERKVKSVQAVPDGGAAPVACPEPKASSCPRGQPRGEPDRSKPLSPTKRKGRSRVPEAVPSSPGSGSRAPAKESPPKRAPAAADSGEEAGRVVPRELTVKSSSLLPEIKPEHKRGPLPHHFDSRGDGGRSRGTGASDADGLKPRNQFGVGRSTVTTKVTLPARPKHVELNLKTPKNPDSLGNEHNSFSQPVHKGNTANKISLFENKRANSSPRHPDVRNTRNNPPSSKTFVGRAKLNLAKKAKEMEQPEKKAVSTSHQNGLVVKEPSAEGKGTAVPVPREEIRPATRGGEGESTKDQVLRPEPEQSDKLDVQTDTAYPSHPGPTALVPVKDSELSGEGQPEAADSKGPVLNNTSDVAQDIAAICDAREAPPKPQDAFSDSQPASAAESARESSLPPSAPISVDAPQDGCVQVPICSVPCTALEVSENHNGCVSPVSPHTEKTPISKPGVGEASPPSTPEHSPETMGKDCPSKVLVQVRSFVLPVENPQDVSSQVISESSEVREAQLPSCHSNEPEVVSVTTCVPQHEDAPDTSPKDIHHREGHVAQSRPHITQSGPEKAPPIQSSSGMPQTAEFILTHSPSSRSHAEGPQRPGQDATSPLTPTRLLNISAGSDNSVLDSSSDMEKFTEIIKKMDSAVCVPQKKKKARVPNSPAPHFAMPPIHEDSLEKVFDPNVFTIGLGKKKESLAEVSPALHLLQNHDPISKLRAKRASTEQSIVFKSLHTNSNGKSEPQTTSEASDKENRDINSGGVKRSRLENSTLFSSMLSSLPQDKVFSPSVTSVNTMSTSFSTSQNSSVSQSSVVQPRKEGGLPCGSDKEQPHLAPHNSLKVFNFNSSNTSRLGSSHMEKYPQKEGSKEDVNSQGNLQLPENNLSDLPKLKNSDGVEGILKSNLPNVGSVDADFLGLFKASRYDPGHSFSGMSLSDSMTLRGSMHSKLNPRPGKVVIFSEPDVSEECIEVFGDIQDCSSWRLSPVIVVKVVRGCWILYEKANFEGHSLALEEGELELSSIWGTEEMLDEEAESDKPVVIGSIRHVVQDYRISQIDLFTEPEGLGLLNSYFDDTEEMQGFPVMQKTCSIKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVIYEKPSFEGRCMELETEMSSFIMEGGETEETTGNNNLPFTSVGSMKVLRGVWVAYEKCGFTGHQYLLEEGEYRDWNAWGGYNGELQSLRPILSDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYVLDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGQSQSFEETIDQIDDSFQTKSCRVLGGSWVAYDGENFSGNQYVLEEGHYPCLSAMGCLPGATLKSLRFIDFEFSEPTIILFERENFKGKKIELNAETVNLRSLGFNTQIRSVRVVGGIWVTYEYGNYRGRQFLLSPAEVPNWYEFSACRQIGSLRPFAQKRIYFRLRNKATGLFMSTNGNLEDLKLLRVQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNVDSQFWCMKSDGRIYSKMKPNLVLDVKGGAQYDQNHIILNTVSTEKLTQVWEAMVL

Target ORF information:

RefSeq Version	NM_001368306.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin beta-gamma domain containing 1 (Crybg1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368306.1

ORF Insert Sequence:

ATGTCCAGAG CACAACCACC AGAAGACACC AGAAGGAAGC CAGTGTTGGA AAAACTTGGC 
ACTCTTTTTA CGGCTGGAAG GAGAAGGAAC ACCAGGAACG GATTAGAGAG TCCCACCAAC 
TCAAATAGCA AATCGGCCTC TGCTAAAGTC CCAAAAACAG GGAGTGAGAA AACTAAGGCT 
CAGGACAACC AACTAAAGGC AACCGACGCT GGCGAGGCGG GCCCCAGGCA GGAGGCTGAG 
GGCCAGCCCG TTGAGAGCTG TGTTCAGGCA ACCCCCTGCG ACGCCGAACA GTCACCTTGC 
TCCAGCATCA ATGTAGGGGT TGTGCAGCAG TGCCACAGCA ATGATTCAGC CCAATTAGAA 
CCTCTACAGG CAGAGGGAGA GACTTTCCCA GATGCCACCA CCGCTGCCAA AGAGCTGCTG 
CATTCCTCAC CAGGGAATCG CTCCAGGCAG GAGAGCGCAG AGACGCCCGC CCGCAGTCCG 
GGGGAGGAAG CGTTGCCTGG CGCTGGCCTC CAACCAGAGA CAGCGAGAGG TGTGTCGGGG 
TCGCCGCCAG AGGAGCACCT CGTGGGAGGA GTAGGAGAGG CCCCCGACGG AGCCCCCGGC 
GTGGGTGCCG AAGCGGGCTC TCTGGGGGCC CCGGGGGACG CGGTTCCTCC AGGTGAGCCT 
CCCGCCCAGG ACGCAGAGAC CAGCCGCACA GCCGGGTCGG TCGACCGCGC GCATCCCACC 
AAAGTGCTAA CGTTGGACAT CTACTTGAGC AAAACTGAGG TGGCTCAGGT GGACGAGCCG 
GCCTCGATTG CTCCGGGGAC AGACGACTGC GAGGATTCGG ACGACATGGA AAAGAGGTCA 
AGCGGCCGCC GGTCTGGTCG GCGGAGGAAG TCGCAGAAAT CCACCGACTC CCCGGGTGCG 
GATGCGGTGG CTCCCGAGAG TGCGGCCAAG GACGAGGCGG TGTTCGACGA CGAGGTGGCG 
CCAGACGCTG CGGCTGAGAA CTGCTTGGCG GAAAGGAAAG TGAAATCGGT ACAAGCGGTT 
CCCGACGGAG GCGCAGCCCC GGTCGCCTGC CCGGAGCCAA AGGCCAGCTC CTGCCCCAGA 
GGGCAACCCC GAGGGGAACC GGACCGCAGC AAGCCCCTGT CGCCCACCAA GCGGAAGGGT 
CGCAGCCGTG TCCCCGAGGC GGTGCCCAGC TCGCCCGGCA GTGGCTCGCG CGCTCCCGCC 
AAGGAGTCCC CACCCAAGAG GGCGCCCGCT GCGGCGGACA GCGGCGAGGA GGCGGGCCGC 
GTCGTCCCGC GCGAGCTCAC CGTCAAAAGC AGCTCGTTGC TGCCCGAGAT CAAGCCAGAG 
CACAAGAGGG GTCCTCTGCC CCACCACTTT GATAGCCGGG GCGACGGGGG TCGCAGCAGG 
GGAACAGGAG CGTCCGACGC CGACGGCCTG AAGCCCAGGA ACCAGTTCGG TGTGGGCAGG 
TCTACTGTGA CCACCAAAGT GACCCTCCCC GCCAGACCCA AACATGTGGA ACTAAATCTG 
AAAACTCCGA AGAATCCTGA CAGTTTGGGA AATGAGCACA ACTCATTTAG CCAGCCAGTT 
CATAAGGGGA ACACAGCCAA CAAAATCTCC TTATTTGAAA ACAAGCGGGC TAACAGTAGC 
CCCCGCCACC CCGACGTCCG TAACACAAGG AACAATCCTC CCTCTAGTAA GACATTTGTT 
GGGAGGGCGA AGCTGAACTT AGCCAAAAAG GCCAAAGAAA TGGAGCAACC CGAAAAGAAA 
GCTGTGTCAA CCAGCCACCA GAATGGCCTG GTGGTCAAAG AGCCATCTGC AGAAGGGAAA 
GGCACAGCAG TCCCTGTCCC CAGAGAAGAG ATTCGTCCAG CCACCAGAGG AGGGGAAGGA 
GAGTCCACCA AGGATCAGGT CCTCCGCCCT GAGCCTGAGC AAAGTGATAA GCTGGATGTA 
CAAACAGACA CTGCCTATCC TTCCCACCCA GGGCCCACTG CTCTGGTTCC TGTGAAGGAC 
TCTGAGCTCT CAGGAGAGGG CCAGCCAGAG GCTGCTGACA GCAAAGGCCC CGTCCTTAAC 
AACACAAGTG ATGTTGCACA GGACATCGCT GCCATTTGTG ATGCCAGAGA GGCTCCCCCA 
AAGCCACAGG ATGCATTTTC TGACTCACAG CCAGCTTCAG CGGCTGAGTC AGCTAGGGAG 
TCATCTCTCC CGCCTTCTGC ACCCATTTCT GTGGACGCTC CCCAAGATGG CTGTGTTCAA 
GTTCCCATAT GCAGTGTTCC CTGCACTGCT CTAGAAGTGT CAGAAAACCA CAATGGGTGT 
GTTTCGCCAG TCTCTCCTCA CACTGAGAAA ACTCCCATCT CCAAACCTGG AGTGGGAGAA 
GCCAGTCCCC CTTCCACTCC AGAGCACAGC CCAGAAACTA TGGGGAAAGA CTGTCCCTCC 
AAAGTCCTCG TCCAGGTCCG GTCCTTTGTG CTTCCCGTGG AGAATCCTCA GGATGTGAGC 
TCTCAGGTCA TCTCAGAGAG CTCTGAAGTT AGAGAGGCAC AGCTGCCAAG CTGTCACAGT 
AATGAGCCTG AAGTGGTGTC TGTCACCACT TGTGTTCCGC AGCACGAGGA TGCACCAGAC 
ACATCACCCA AGGACATCCA TCACAGAGAA GGACATGTGG CACAGTCTCG ACCACACATC 
ACACAGTCAG GACCTGAGAA GGCTCCGCCC ATCCAGAGCA GCAGTGGGAT GCCCCAGACA 
GCGGAGTTCA TCCTCACCCA CTCTCCCAGC AGCAGAAGTC ATGCAGAAGG CCCCCAAAGG 
CCAGGTCAAG ATGCGACCAG CCCACTTACC CCTACCAGGC TTTTGAACAT TTCTGCTGGT 
AGTGACAATA GTGTCTTGGA TTCTTCTTCT GATATGGAAA AATTCACTGA AATTATTAAA 
AAGATGGACA GTGCAGTATG TGTGCCCCAG AAAAAGAAGA AGGCCCGGGT CCCAAACTCT 
CCAGCCCCTC ACTTCGCTAT GCCTCCCATT CACGAGGACA GTTTGGAAAA GGTGTTTGAT 
CCCAACGTGT TCACCATTGG TTTAGGGAAG AAGAAGGAAA GCCTGGCAGA GGTGTCACCA 
GCTTTGCACT TGTTGCAGAA CCACGACCCC ATATCCAAAC TGAGAGCCAA GCGTGCATCC 
ACCGAGCAGA GCATCGTCTT CAAGTCCTTG CACACTAACA GTAATGGGAA GAGCGAGCCA 
CAGACCACTT CAGAAGCAAG TGACAAGGAG AACAGGGACA TCAACAGCGG CGGCGTTAAG 
AGGTCACGGC TAGAAAACAG CACGCTTTTC TCAAGCATGC TTTCTTCTCT GCCACAAGAC 
AAAGTCTTTT CTCCCTCCGT GACGTCAGTC AACACAATGA GCACATCTTT TAGCACTTCT 
CAGAACAGCT CCGTATCTCA GTCATCAGTG GTACAGCCCA GGAAGGAGGG TGGCCTGCCC 
TGTGGCTCAG ACAAAGAACA GCCACACCTT GCACCCCACA ACTCCTTAAA GGTCTTCAAT 
TTCAATTCAT CAAATACATC TCGTCTGGGT TCCAGCCACA TGGAAAAGTA CCCACAAAAA 
GAGGGAAGCA AGGAAGATGT GAATTCTCAG GGCAACCTAC AATTGCCAGA AAACAACCTT 
TCAGACTTAC CCAAACTGAA GAACAGCGAT GGCGTGGAAG GTATTCTGAA GTCAAACCTG 
CCGAATGTTG GGAGCGTGGA CGCGGACTTC CTGGGCCTTT TCAAAGCAAG CCGGTATGAC 
CCAGGCCATT CCTTTTCTGG AATGTCCTTA TCAGACTCTA TGACTCTTAG AGGAAGTATG 
CACAGCAAAC TCAACCCCCG GCCTGGCAAG GTAGTGATAT TCAGTGAGCC CGACGTCTCT 
GAGGAGTGCA TTGAGGTGTT TGGGGACATC CAGGACTGCA GCTCCTGGCG CCTCTCTCCA 
GTGATAGTAG TGAAAGTTGT CCGAGGATGT TGGATTTTGT ATGAGAAAGC CAATTTTGAA 
GGGCATTCTC TTGCTTTAGA AGAAGGTGAA CTGGAACTCT CTAGTATCTG GGGAACAGAG 
GAAATGTTGG ATGAAGAAGC AGAGTCTGAT AAACCTGTAG TGATTGGTTC CATCAGACAC 
GTGGTTCAGG ATTACAGAAT TAGTCAGATC GACTTGTTTA CTGAGCCTGA AGGGTTAGGA 
CTCCTGAACT CGTACTTTGA TGACACTGAG GAAATGCAGG GCTTCCCTGT GATGCAGAAG 
ACGTGTTCCA TCAAAGTGCA CTGGGGCACA TGGCTGATCT ATGAAGAGCC TGGATTCCAA 
GGTGTCCCCT TCATTCTGGA GCCCGGAGAA TACCCTGACC TATCCTTCTG GGATACCGAG 
GAAGCGTACA TCGGATCCAT GCGGCCTCTG AAAATGGGTG GCCGTAAAGT TGAATTCCCT 
ACAGATCCAA AGGTAGTTAT TTATGAAAAG CCTTCCTTTG AAGGAAGGTG TATGGAGCTA 
GAAACAGAAA TGAGTAGTTT TATCATGGAA GGAGGTGAGA CAGAAGAAAC AACTGGAAAC 
AACAACTTGC CCTTTACATC CGTGGGGTCT ATGAAAGTCC TAAGAGGCGT CTGGGTTGCT 
TATGAGAAGT GTGGCTTCAC TGGCCACCAG TATTTGCTGG AGGAGGGAGA ATACAGGGAC 
TGGAACGCCT GGGGAGGCTA CAATGGAGAG CTCCAGTCTC TGCGGCCTAT ATTAAGTGAT 
TTTTCAAATG CGCACATGAT AATGTACAGT GAGAAGAACT TTGGCTCCAA AGGTTCCAGT 
ATTGATGTGT TGGGAATTGT TGCCAATTTG AAGGAGACCG GCTACGGAGT GAAGACACAG 
TCAATCAATG TACTGAGTGG CGTGTGGGTA GCCTATGAAA ACCCGGACTT CACGGGGGAG 
CAGTATGTCC TGGATAAAGG CTTCTACACC AGTTTTGAGG ATTGGGGAGG CAAAAATTGT 
AAGATCTCTT CCGTCCAACC CATATGCCTG GATTCTTTCA CTGGCCCCAG GAGACGAAAT 
CAGATTCACT TGTTTTCAGA ACCACAGTTC CAAGGTCAAA GTCAAAGTTT TGAAGAAACA 
ATAGATCAGA TTGACGACTC CTTTCAGACC AAGTCTTGCA GAGTTTTGGG AGGCAGCTGG 
GTTGCTTATG ATGGAGAAAA TTTCTCTGGC AATCAGTATG TACTGGAAGA AGGCCACTAT 
CCTTGTCTCT CGGCAATGGG ATGCCTGCCT GGAGCAACCC TGAAGTCTCT TCGCTTTATA 
GATTTTGAAT TTTCCGAACC AACAATTATT CTTTTCGAAA GAGAAAACTT CAAAGGAAAA 
AAGATAGAAC TGAACGCAGA AACAGTCAAT CTCCGATCCT TGGGGTTCAA CACTCAGATC 
CGCTCTGTGC GGGTCGTTGG TGGCATATGG GTTACCTATG AATATGGCAA TTACAGAGGC 
CGGCAGTTCC TGCTGTCTCC TGCAGAAGTA CCAAACTGGT ATGAGTTCAG TGCCTGTCGC 
CAAATAGGCT CCCTCCGGCC CTTTGCTCAG AAACGAATTT ACTTCAGACT TCGAAACAAA 
GCAACAGGAC TATTTATGTC AACCAACGGG AACTTGGAAG ACCTGAAGCT TCTCCGAGTC 
CAGGTCATGG AAGATGTTGG TGCTGATGAT CAGATCTGGA TCTATCAAGA AGGGTGCATC 
AAATGCAGGA TAGCAGAAGA CTGCTGCCTG ACGATCGTGG GCAGCCTCGT CACGTCTGGC 
TCCAAACTAG GACTGGCCCT CGATCAAAAT GTCGACAGTC AGTTCTGGTG CATGAAGTCC 
GACGGCAGGA TCTACAGCAA GATGAAACCA AATTTAGTGT TGGATGTGAA AGGGGGTGCG 
CAGTATGATC AAAATCACAT CATCCTCAAC ACAGTGAGCA CAGAGAAGTT AACACAAGTG 
TGGGAAGCCA TGGTCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aim1 ( XM_006512499.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512562.1 Mus musculus absent in melanoma 1 (Aim1), transcript variant X2, mRNA.

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CloneID	OMu43696
Clone ID Related Accession (Same CDS sequence)	NM_001368306.1 , XM_006512499.3
Accession Version	XM_006512499.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	absent in melanoma 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512499.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAG CACAACCACC AGAAGACACC AGAAGGAAGC CAGTGTTGGA AAAACTTGGC 
ACTCTTTTTA CGGCTGGAAG GAGAAGGAAC ACCAGGAACG GATTAGAGAG TCCCACCAAC 
TCAAATAGCA AATCGGCCTC TGCTAAAGTC CCAAAAACAG GGAGTGAGAA AACTAAGGCT 
CAGGACAACC AACTAAAGGC AACCGACGCT GGCGAGGCGG GCCCCAGGCA GGAGGCTGAG 
GGCCAGCCCG TTGAGAGCTG TGTTCAGGCA ACCCCCTGCG ACGCCGAACA GTCACCTTGC 
TCCAGCATCA ATGTAGGGGT TGTGCAGCAG TGCCACAGCA ATGATTCAGC CCAATTAGAA 
CCTCTACAGG CAGAGGGAGA GACTTTCCCA GATGCCACCA CCGCTGCCAA AGAGCTGCTG 
CATTCCTCAC CAGGGAATCG CTCCAGGCAG GAGAGCGCAG AGACGCCCGC CCGCAGTCCG 
GGGGAGGAAG CGTTGCCTGG CGCTGGCCTC CAACCAGAGA CAGCGAGAGG TGTGTCGGGG 
TCGCCGCCAG AGGAGCACCT CGTGGGAGGA GTAGGAGAGG CCCCCGACGG AGCCCCCGGC 
GTGGGTGCCG AAGCGGGCTC TCTGGGGGCC CCGGGGGACG CGGTTCCTCC AGGTGAGCCT 
CCCGCCCAGG ACGCAGAGAC CAGCCGCACA GCCGGGTCGG TCGACCGCGC GCATCCCACC 
AAAGTGCTAA CGTTGGACAT CTACTTGAGC AAAACTGAGG TGGCTCAGGT GGACGAGCCG 
GCCTCGATTG CTCCGGGGAC AGACGACTGC GAGGATTCGG ACGACATGGA AAAGAGGTCA 
AGCGGCCGCC GGTCTGGTCG GCGGAGGAAG TCGCAGAAAT CCACCGACTC CCCGGGTGCG 
GATGCGGTGG CTCCCGAGAG TGCGGCCAAG GACGAGGCGG TGTTCGACGA CGAGGTGGCG 
CCAGACGCTG CGGCTGAGAA CTGCTTGGCG GAAAGGAAAG TGAAATCGGT ACAAGCGGTT 
CCCGACGGAG GCGCAGCCCC GGTCGCCTGC CCGGAGCCAA AGGCCAGCTC CTGCCCCAGA 
GGGCAACCCC GAGGGGAACC GGACCGCAGC AAGCCCCTGT CGCCCACCAA GCGGAAGGGT 
CGCAGCCGTG TCCCCGAGGC GGTGCCCAGC TCGCCCGGCA GTGGCTCGCG CGCTCCCGCC 
AAGGAGTCCC CACCCAAGAG GGCGCCCGCT GCGGCGGACA GCGGCGAGGA GGCGGGCCGC 
GTCGTCCCGC GCGAGCTCAC CGTCAAAAGC AGCTCGTTGC TGCCCGAGAT CAAGCCAGAG 
CACAAGAGGG GTCCTCTGCC CCACCACTTT GATAGCCGGG GCGACGGGGG TCGCAGCAGG 
GGAACAGGAG CGTCCGACGC CGACGGCCTG AAGCCCAGGA ACCAGTTCGG TGTGGGCAGG 
TCTACTGTGA CCACCAAAGT GACCCTCCCC GCCAGACCCA AACATGTGGA ACTAAATCTG 
AAAACTCCGA AGAATCCTGA CAGTTTGGGA AATGAGCACA ACTCATTTAG CCAGCCAGTT 
CATAAGGGGA ACACAGCCAA CAAAATCTCC TTATTTGAAA ACAAGCGGGC TAACAGTAGC 
CCCCGCCACC CCGACGTCCG TAACACAAGG AACAATCCTC CCTCTAGTAA GACATTTGTT 
GGGAGGGCGA AGCTGAACTT AGCCAAAAAG GCCAAAGAAA TGGAGCAACC CGAAAAGAAA 
GCTGTGTCAA CCAGCCACCA GAATGGCCTG GTGGTCAAAG AGCCATCTGC AGAAGGGAAA 
GGCACAGCAG TCCCTGTCCC CAGAGAAGAG ATTCGTCCAG CCACCAGAGG AGGGGAAGGA 
GAGTCCACCA AGGATCAGGT CCTCCGCCCT GAGCCTGAGC AAAGTGATAA GCTGGATGTA 
CAAACAGACA CTGCCTATCC TTCCCACCCA GGGCCCACTG CTCTGGTTCC TGTGAAGGAC 
TCTGAGCTCT CAGGAGAGGG CCAGCCAGAG GCTGCTGACA GCAAAGGCCC CGTCCTTAAC 
AACACAAGTG ATGTTGCACA GGACATCGCT GCCATTTGTG ATGCCAGAGA GGCTCCCCCA 
AAGCCACAGG ATGCATTTTC TGACTCACAG CCAGCTTCAG CGGCTGAGTC AGCTAGGGAG 
TCATCTCTCC CGCCTTCTGC ACCCATTTCT GTGGACGCTC CCCAAGATGG CTGTGTTCAA 
GTTCCCATAT GCAGTGTTCC CTGCACTGCT CTAGAAGTGT CAGAAAACCA CAATGGGTGT 
GTTTCGCCAG TCTCTCCTCA CACTGAGAAA ACTCCCATCT CCAAACCTGG AGTGGGAGAA 
GCCAGTCCCC CTTCCACTCC AGAGCACAGC CCAGAAACTA TGGGGAAAGA CTGTCCCTCC 
AAAGTCCTCG TCCAGGTCCG GTCCTTTGTG CTTCCCGTGG AGAATCCTCA GGATGTGAGC 
TCTCAGGTCA TCTCAGAGAG CTCTGAAGTT AGAGAGGCAC AGCTGCCAAG CTGTCACAGT 
AATGAGCCTG AAGTGGTGTC TGTCACCACT TGTGTTCCGC AGCACGAGGA TGCACCAGAC 
ACATCACCCA AGGACATCCA TCACAGAGAA GGACATGTGG CACAGTCTCG ACCACACATC 
ACACAGTCAG GACCTGAGAA GGCTCCGCCC ATCCAGAGCA GCAGTGGGAT GCCCCAGACA 
GCGGAGTTCA TCCTCACCCA CTCTCCCAGC AGCAGAAGTC ATGCAGAAGG CCCCCAAAGG 
CCAGGTCAAG ATGCGACCAG CCCACTTACC CCTACCAGGC TTTTGAACAT TTCTGCTGGT 
AGTGACAATA GTGTCTTGGA TTCTTCTTCT GATATGGAAA AATTCACTGA AATTATTAAA 
AAGATGGACA GTGCAGTATG TGTGCCCCAG AAAAAGAAGA AGGCCCGGGT CCCAAACTCT 
CCAGCCCCTC ACTTCGCTAT GCCTCCCATT CACGAGGACA GTTTGGAAAA GGTGTTTGAT 
CCCAACGTGT TCACCATTGG TTTAGGGAAG AAGAAGGAAA GCCTGGCAGA GGTGTCACCA 
GCTTTGCACT TGTTGCAGAA CCACGACCCC ATATCCAAAC TGAGAGCCAA GCGTGCATCC 
ACCGAGCAGA GCATCGTCTT CAAGTCCTTG CACACTAACA GTAATGGGAA GAGCGAGCCA 
CAGACCACTT CAGAAGCAAG TGACAAGGAG AACAGGGACA TCAACAGCGG CGGCGTTAAG 
AGGTCACGGC TAGAAAACAG CACGCTTTTC TCAAGCATGC TTTCTTCTCT GCCACAAGAC 
AAAGTCTTTT CTCCCTCCGT GACGTCAGTC AACACAATGA GCACATCTTT TAGCACTTCT 
CAGAACAGCT CCGTATCTCA GTCATCAGTG GTACAGCCCA GGAAGGAGGG TGGCCTGCCC 
TGTGGCTCAG ACAAAGAACA GCCACACCTT GCACCCCACA ACTCCTTAAA GGTCTTCAAT 
TTCAATTCAT CAAATACATC TCGTCTGGGT TCCAGCCACA TGGAAAAGTA CCCACAAAAA 
GAGGGAAGCA AGGAAGATGT GAATTCTCAG GGCAACCTAC AATTGCCAGA AAACAACCTT 
TCAGACTTAC CCAAACTGAA GAACAGCGAT GGCGTGGAAG GTATTCTGAA GTCAAACCTG 
CCGAATGTTG GGAGCGTGGA CGCGGACTTC CTGGGCCTTT TCAAAGCAAG CCGGTATGAC 
CCAGGCCATT CCTTTTCTGG AATGTCCTTA TCAGACTCTA TGACTCTTAG AGGAAGTATG 
CACAGCAAAC TCAACCCCCG GCCTGGCAAG GTAGTGATAT TCAGTGAGCC CGACGTCTCT 
GAGGAGTGCA TTGAGGTGTT TGGGGACATC CAGGACTGCA GCTCCTGGCG CCTCTCTCCA 
GTGATAGTAG TGAAAGTTGT CCGAGGATGT TGGATTTTGT ATGAGAAAGC CAATTTTGAA 
GGGCATTCTC TTGCTTTAGA AGAAGGTGAA CTGGAACTCT CTAGTATCTG GGGAACAGAG 
GAAATGTTGG ATGAAGAAGC AGAGTCTGAT AAACCTGTAG TGATTGGTTC CATCAGACAC 
GTGGTTCAGG ATTACAGAAT TAGTCAGATC GACTTGTTTA CTGAGCCTGA AGGGTTAGGA 
CTCCTGAACT CGTACTTTGA TGACACTGAG GAAATGCAGG GCTTCCCTGT GATGCAGAAG 
ACGTGTTCCA TCAAAGTGCA CTGGGGCACA TGGCTGATCT ATGAAGAGCC TGGATTCCAA 
GGTGTCCCCT TCATTCTGGA GCCCGGAGAA TACCCTGACC TATCCTTCTG GGATACCGAG 
GAAGCGTACA TCGGATCCAT GCGGCCTCTG AAAATGGGTG GCCGTAAAGT TGAATTCCCT 
ACAGATCCAA AGGTAGTTAT TTATGAAAAG CCTTCCTTTG AAGGAAGGTG TATGGAGCTA 
GAAACAGAAA TGAGTAGTTT TATCATGGAA GGAGGTGAGA CAGAAGAAAC AACTGGAAAC 
AACAACTTGC CCTTTACATC CGTGGGGTCT ATGAAAGTCC TAAGAGGCGT CTGGGTTGCT 
TATGAGAAGT GTGGCTTCAC TGGCCACCAG TATTTGCTGG AGGAGGGAGA ATACAGGGAC 
TGGAACGCCT GGGGAGGCTA CAATGGAGAG CTCCAGTCTC TGCGGCCTAT ATTAAGTGAT 
TTTTCAAATG CGCACATGAT AATGTACAGT GAGAAGAACT TTGGCTCCAA AGGTTCCAGT 
ATTGATGTGT TGGGAATTGT TGCCAATTTG AAGGAGACCG GCTACGGAGT GAAGACACAG 
TCAATCAATG TACTGAGTGG CGTGTGGGTA GCCTATGAAA ACCCGGACTT CACGGGGGAG 
CAGTATGTCC TGGATAAAGG CTTCTACACC AGTTTTGAGG ATTGGGGAGG CAAAAATTGT 
AAGATCTCTT CCGTCCAACC CATATGCCTG GATTCTTTCA CTGGCCCCAG GAGACGAAAT 
CAGATTCACT TGTTTTCAGA ACCACAGTTC CAAGGTCAAA GTCAAAGTTT TGAAGAAACA 
ATAGATCAGA TTGACGACTC CTTTCAGACC AAGTCTTGCA GAGTTTTGGG AGGCAGCTGG 
GTTGCTTATG ATGGAGAAAA TTTCTCTGGC AATCAGTATG TACTGGAAGA AGGCCACTAT 
CCTTGTCTCT CGGCAATGGG ATGCCTGCCT GGAGCAACCC TGAAGTCTCT TCGCTTTATA 
GATTTTGAAT TTTCCGAACC AACAATTATT CTTTTCGAAA GAGAAAACTT CAAAGGAAAA 
AAGATAGAAC TGAACGCAGA AACAGTCAAT CTCCGATCCT TGGGGTTCAA CACTCAGATC 
CGCTCTGTGC GGGTCGTTGG TGGCATATGG GTTACCTATG AATATGGCAA TTACAGAGGC 
CGGCAGTTCC TGCTGTCTCC TGCAGAAGTA CCAAACTGGT ATGAGTTCAG TGCCTGTCGC 
CAAATAGGCT CCCTCCGGCC CTTTGCTCAG AAACGAATTT ACTTCAGACT TCGAAACAAA 
GCAACAGGAC TATTTATGTC AACCAACGGG AACTTGGAAG ACCTGAAGCT TCTCCGAGTC 
CAGGTCATGG AAGATGTTGG TGCTGATGAT CAGATCTGGA TCTATCAAGA AGGGTGCATC 
AAATGCAGGA TAGCAGAAGA CTGCTGCCTG ACGATCGTGG GCAGCCTCGT CACGTCTGGC 
TCCAAACTAG GACTGGCCCT CGATCAAAAT GTCGACAGTC AGTTCTGGTG CATGAAGTCC 
GACGGCAGGA TCTACAGCAA GATGAAACCA AATTTAGTGT TGGATGTGAA AGGGGGTGCG 
CAGTATGATC AAAATCACAT CATCCTCAAC ACAGTGAGCA CAGAGAAGTT AACACAAGTG 
TGGGAAGCCA TGGTCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512562.1
CDS	163..6063
Translation	MSRAQPPEDTRRKPVLEKLGTLFTAGRRRNTRNGLESPTNSNSKSASAKVPKTGSEKTKAQDNQLKATDAGEAGPRQEAEGQPVESCVQATPCDAEQSPCSSINVGVVQQCHSNDSAQLEPLQAEGETFPDATTAAKELLHSSPGNRSRQESAETPARSPGEEALPGAGLQPETARGVSGSPPEEHLVGGVGEAPDGAPGVGAEAGSLGAPGDAVPPGEPPAQDAETSRTAGSVDRAHPTKVLTLDIYLSKTEVAQVDEPASIAPGTDDCEDSDDMEKRSSGRRSGRRRKSQKSTDSPGADAVAPESAAKDEAVFDDEVAPDAAAENCLAERKVKSVQAVPDGGAAPVACPEPKASSCPRGQPRGEPDRSKPLSPTKRKGRSRVPEAVPSSPGSGSRAPAKESPPKRAPAAADSGEEAGRVVPRELTVKSSSLLPEIKPEHKRGPLPHHFDSRGDGGRSRGTGASDADGLKPRNQFGVGRSTVTTKVTLPARPKHVELNLKTPKNPDSLGNEHNSFSQPVHKGNTANKISLFENKRANSSPRHPDVRNTRNNPPSSKTFVGRAKLNLAKKAKEMEQPEKKAVSTSHQNGLVVKEPSAEGKGTAVPVPREEIRPATRGGEGESTKDQVLRPEPEQSDKLDVQTDTAYPSHPGPTALVPVKDSELSGEGQPEAADSKGPVLNNTSDVAQDIAAICDAREAPPKPQDAFSDSQPASAAESARESSLPPSAPISVDAPQDGCVQVPICSVPCTALEVSENHNGCVSPVSPHTEKTPISKPGVGEASPPSTPEHSPETMGKDCPSKVLVQVRSFVLPVENPQDVSSQVISESSEVREAQLPSCHSNEPEVVSVTTCVPQHEDAPDTSPKDIHHREGHVAQSRPHITQSGPEKAPPIQSSSGMPQTAEFILTHSPSSRSHAEGPQRPGQDATSPLTPTRLLNISAGSDNSVLDSSSDMEKFTEIIKKMDSAVCVPQKKKKARVPNSPAPHFAMPPIHEDSLEKVFDPNVFTIGLGKKKESLAEVSPALHLLQNHDPISKLRAKRASTEQSIVFKSLHTNSNGKSEPQTTSEASDKENRDINSGGVKRSRLENSTLFSSMLSSLPQDKVFSPSVTSVNTMSTSFSTSQNSSVSQSSVVQPRKEGGLPCGSDKEQPHLAPHNSLKVFNFNSSNTSRLGSSHMEKYPQKEGSKEDVNSQGNLQLPENNLSDLPKLKNSDGVEGILKSNLPNVGSVDADFLGLFKASRYDPGHSFSGMSLSDSMTLRGSMHSKLNPRPGKVVIFSEPDVSEECIEVFGDIQDCSSWRLSPVIVVKVVRGCWILYEKANFEGHSLALEEGELELSSIWGTEEMLDEEAESDKPVVIGSIRHVVQDYRISQIDLFTEPEGLGLLNSYFDDTEEMQGFPVMQKTCSIKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVIYEKPSFEGRCMELETEMSSFIMEGGETEETTGNNNLPFTSVGSMKVLRGVWVAYEKCGFTGHQYLLEEGEYRDWNAWGGYNGELQSLRPILSDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYVLDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGQSQSFEETIDQIDDSFQTKSCRVLGGSWVAYDGENFSGNQYVLEEGHYPCLSAMGCLPGATLKSLRFIDFEFSEPTIILFERENFKGKKIELNAETVNLRSLGFNTQIRSVRVVGGIWVTYEYGNYRGRQFLLSPAEVPNWYEFSACRQIGSLRPFAQKRIYFRLRNKATGLFMSTNGNLEDLKLLRVQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNVDSQFWCMKSDGRIYSKMKPNLVLDVKGGAQYDQNHIILNTVSTEKLTQVWEAMVL

Target ORF information:

RefSeq Version	XM_006512499.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus absent in melanoma 1 (Aim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512499.3

ORF Insert Sequence:

ATGTCCAGAG CACAACCACC AGAAGACACC AGAAGGAAGC CAGTGTTGGA AAAACTTGGC 
ACTCTTTTTA CGGCTGGAAG GAGAAGGAAC ACCAGGAACG GATTAGAGAG TCCCACCAAC 
TCAAATAGCA AATCGGCCTC TGCTAAAGTC CCAAAAACAG GGAGTGAGAA AACTAAGGCT 
CAGGACAACC AACTAAAGGC AACCGACGCT GGCGAGGCGG GCCCCAGGCA GGAGGCTGAG 
GGCCAGCCCG TTGAGAGCTG TGTTCAGGCA ACCCCCTGCG ACGCCGAACA GTCACCTTGC 
TCCAGCATCA ATGTAGGGGT TGTGCAGCAG TGCCACAGCA ATGATTCAGC CCAATTAGAA 
CCTCTACAGG CAGAGGGAGA GACTTTCCCA GATGCCACCA CCGCTGCCAA AGAGCTGCTG 
CATTCCTCAC CAGGGAATCG CTCCAGGCAG GAGAGCGCAG AGACGCCCGC CCGCAGTCCG 
GGGGAGGAAG CGTTGCCTGG CGCTGGCCTC CAACCAGAGA CAGCGAGAGG TGTGTCGGGG 
TCGCCGCCAG AGGAGCACCT CGTGGGAGGA GTAGGAGAGG CCCCCGACGG AGCCCCCGGC 
GTGGGTGCCG AAGCGGGCTC TCTGGGGGCC CCGGGGGACG CGGTTCCTCC AGGTGAGCCT 
CCCGCCCAGG ACGCAGAGAC CAGCCGCACA GCCGGGTCGG TCGACCGCGC GCATCCCACC 
AAAGTGCTAA CGTTGGACAT CTACTTGAGC AAAACTGAGG TGGCTCAGGT GGACGAGCCG 
GCCTCGATTG CTCCGGGGAC AGACGACTGC GAGGATTCGG ACGACATGGA AAAGAGGTCA 
AGCGGCCGCC GGTCTGGTCG GCGGAGGAAG TCGCAGAAAT CCACCGACTC CCCGGGTGCG 
GATGCGGTGG CTCCCGAGAG TGCGGCCAAG GACGAGGCGG TGTTCGACGA CGAGGTGGCG 
CCAGACGCTG CGGCTGAGAA CTGCTTGGCG GAAAGGAAAG TGAAATCGGT ACAAGCGGTT 
CCCGACGGAG GCGCAGCCCC GGTCGCCTGC CCGGAGCCAA AGGCCAGCTC CTGCCCCAGA 
GGGCAACCCC GAGGGGAACC GGACCGCAGC AAGCCCCTGT CGCCCACCAA GCGGAAGGGT 
CGCAGCCGTG TCCCCGAGGC GGTGCCCAGC TCGCCCGGCA GTGGCTCGCG CGCTCCCGCC 
AAGGAGTCCC CACCCAAGAG GGCGCCCGCT GCGGCGGACA GCGGCGAGGA GGCGGGCCGC 
GTCGTCCCGC GCGAGCTCAC CGTCAAAAGC AGCTCGTTGC TGCCCGAGAT CAAGCCAGAG 
CACAAGAGGG GTCCTCTGCC CCACCACTTT GATAGCCGGG GCGACGGGGG TCGCAGCAGG 
GGAACAGGAG CGTCCGACGC CGACGGCCTG AAGCCCAGGA ACCAGTTCGG TGTGGGCAGG 
TCTACTGTGA CCACCAAAGT GACCCTCCCC GCCAGACCCA AACATGTGGA ACTAAATCTG 
AAAACTCCGA AGAATCCTGA CAGTTTGGGA AATGAGCACA ACTCATTTAG CCAGCCAGTT 
CATAAGGGGA ACACAGCCAA CAAAATCTCC TTATTTGAAA ACAAGCGGGC TAACAGTAGC 
CCCCGCCACC CCGACGTCCG TAACACAAGG AACAATCCTC CCTCTAGTAA GACATTTGTT 
GGGAGGGCGA AGCTGAACTT AGCCAAAAAG GCCAAAGAAA TGGAGCAACC CGAAAAGAAA 
GCTGTGTCAA CCAGCCACCA GAATGGCCTG GTGGTCAAAG AGCCATCTGC AGAAGGGAAA 
GGCACAGCAG TCCCTGTCCC CAGAGAAGAG ATTCGTCCAG CCACCAGAGG AGGGGAAGGA 
GAGTCCACCA AGGATCAGGT CCTCCGCCCT GAGCCTGAGC AAAGTGATAA GCTGGATGTA 
CAAACAGACA CTGCCTATCC TTCCCACCCA GGGCCCACTG CTCTGGTTCC TGTGAAGGAC 
TCTGAGCTCT CAGGAGAGGG CCAGCCAGAG GCTGCTGACA GCAAAGGCCC CGTCCTTAAC 
AACACAAGTG ATGTTGCACA GGACATCGCT GCCATTTGTG ATGCCAGAGA GGCTCCCCCA 
AAGCCACAGG ATGCATTTTC TGACTCACAG CCAGCTTCAG CGGCTGAGTC AGCTAGGGAG 
TCATCTCTCC CGCCTTCTGC ACCCATTTCT GTGGACGCTC CCCAAGATGG CTGTGTTCAA 
GTTCCCATAT GCAGTGTTCC CTGCACTGCT CTAGAAGTGT CAGAAAACCA CAATGGGTGT 
GTTTCGCCAG TCTCTCCTCA CACTGAGAAA ACTCCCATCT CCAAACCTGG AGTGGGAGAA 
GCCAGTCCCC CTTCCACTCC AGAGCACAGC CCAGAAACTA TGGGGAAAGA CTGTCCCTCC 
AAAGTCCTCG TCCAGGTCCG GTCCTTTGTG CTTCCCGTGG AGAATCCTCA GGATGTGAGC 
TCTCAGGTCA TCTCAGAGAG CTCTGAAGTT AGAGAGGCAC AGCTGCCAAG CTGTCACAGT 
AATGAGCCTG AAGTGGTGTC TGTCACCACT TGTGTTCCGC AGCACGAGGA TGCACCAGAC 
ACATCACCCA AGGACATCCA TCACAGAGAA GGACATGTGG CACAGTCTCG ACCACACATC 
ACACAGTCAG GACCTGAGAA GGCTCCGCCC ATCCAGAGCA GCAGTGGGAT GCCCCAGACA 
GCGGAGTTCA TCCTCACCCA CTCTCCCAGC AGCAGAAGTC ATGCAGAAGG CCCCCAAAGG 
CCAGGTCAAG ATGCGACCAG CCCACTTACC CCTACCAGGC TTTTGAACAT TTCTGCTGGT 
AGTGACAATA GTGTCTTGGA TTCTTCTTCT GATATGGAAA AATTCACTGA AATTATTAAA 
AAGATGGACA GTGCAGTATG TGTGCCCCAG AAAAAGAAGA AGGCCCGGGT CCCAAACTCT 
CCAGCCCCTC ACTTCGCTAT GCCTCCCATT CACGAGGACA GTTTGGAAAA GGTGTTTGAT 
CCCAACGTGT TCACCATTGG TTTAGGGAAG AAGAAGGAAA GCCTGGCAGA GGTGTCACCA 
GCTTTGCACT TGTTGCAGAA CCACGACCCC ATATCCAAAC TGAGAGCCAA GCGTGCATCC 
ACCGAGCAGA GCATCGTCTT CAAGTCCTTG CACACTAACA GTAATGGGAA GAGCGAGCCA 
CAGACCACTT CAGAAGCAAG TGACAAGGAG AACAGGGACA TCAACAGCGG CGGCGTTAAG 
AGGTCACGGC TAGAAAACAG CACGCTTTTC TCAAGCATGC TTTCTTCTCT GCCACAAGAC 
AAAGTCTTTT CTCCCTCCGT GACGTCAGTC AACACAATGA GCACATCTTT TAGCACTTCT 
CAGAACAGCT CCGTATCTCA GTCATCAGTG GTACAGCCCA GGAAGGAGGG TGGCCTGCCC 
TGTGGCTCAG ACAAAGAACA GCCACACCTT GCACCCCACA ACTCCTTAAA GGTCTTCAAT 
TTCAATTCAT CAAATACATC TCGTCTGGGT TCCAGCCACA TGGAAAAGTA CCCACAAAAA 
GAGGGAAGCA AGGAAGATGT GAATTCTCAG GGCAACCTAC AATTGCCAGA AAACAACCTT 
TCAGACTTAC CCAAACTGAA GAACAGCGAT GGCGTGGAAG GTATTCTGAA GTCAAACCTG 
CCGAATGTTG GGAGCGTGGA CGCGGACTTC CTGGGCCTTT TCAAAGCAAG CCGGTATGAC 
CCAGGCCATT CCTTTTCTGG AATGTCCTTA TCAGACTCTA TGACTCTTAG AGGAAGTATG 
CACAGCAAAC TCAACCCCCG GCCTGGCAAG GTAGTGATAT TCAGTGAGCC CGACGTCTCT 
GAGGAGTGCA TTGAGGTGTT TGGGGACATC CAGGACTGCA GCTCCTGGCG CCTCTCTCCA 
GTGATAGTAG TGAAAGTTGT CCGAGGATGT TGGATTTTGT ATGAGAAAGC CAATTTTGAA 
GGGCATTCTC TTGCTTTAGA AGAAGGTGAA CTGGAACTCT CTAGTATCTG GGGAACAGAG 
GAAATGTTGG ATGAAGAAGC AGAGTCTGAT AAACCTGTAG TGATTGGTTC CATCAGACAC 
GTGGTTCAGG ATTACAGAAT TAGTCAGATC GACTTGTTTA CTGAGCCTGA AGGGTTAGGA 
CTCCTGAACT CGTACTTTGA TGACACTGAG GAAATGCAGG GCTTCCCTGT GATGCAGAAG 
ACGTGTTCCA TCAAAGTGCA CTGGGGCACA TGGCTGATCT ATGAAGAGCC TGGATTCCAA 
GGTGTCCCCT TCATTCTGGA GCCCGGAGAA TACCCTGACC TATCCTTCTG GGATACCGAG 
GAAGCGTACA TCGGATCCAT GCGGCCTCTG AAAATGGGTG GCCGTAAAGT TGAATTCCCT 
ACAGATCCAA AGGTAGTTAT TTATGAAAAG CCTTCCTTTG AAGGAAGGTG TATGGAGCTA 
GAAACAGAAA TGAGTAGTTT TATCATGGAA GGAGGTGAGA CAGAAGAAAC AACTGGAAAC 
AACAACTTGC CCTTTACATC CGTGGGGTCT ATGAAAGTCC TAAGAGGCGT CTGGGTTGCT 
TATGAGAAGT GTGGCTTCAC TGGCCACCAG TATTTGCTGG AGGAGGGAGA ATACAGGGAC 
TGGAACGCCT GGGGAGGCTA CAATGGAGAG CTCCAGTCTC TGCGGCCTAT ATTAAGTGAT 
TTTTCAAATG CGCACATGAT AATGTACAGT GAGAAGAACT TTGGCTCCAA AGGTTCCAGT 
ATTGATGTGT TGGGAATTGT TGCCAATTTG AAGGAGACCG GCTACGGAGT GAAGACACAG 
TCAATCAATG TACTGAGTGG CGTGTGGGTA GCCTATGAAA ACCCGGACTT CACGGGGGAG 
CAGTATGTCC TGGATAAAGG CTTCTACACC AGTTTTGAGG ATTGGGGAGG CAAAAATTGT 
AAGATCTCTT CCGTCCAACC CATATGCCTG GATTCTTTCA CTGGCCCCAG GAGACGAAAT 
CAGATTCACT TGTTTTCAGA ACCACAGTTC CAAGGTCAAA GTCAAAGTTT TGAAGAAACA 
ATAGATCAGA TTGACGACTC CTTTCAGACC AAGTCTTGCA GAGTTTTGGG AGGCAGCTGG 
GTTGCTTATG ATGGAGAAAA TTTCTCTGGC AATCAGTATG TACTGGAAGA AGGCCACTAT 
CCTTGTCTCT CGGCAATGGG ATGCCTGCCT GGAGCAACCC TGAAGTCTCT TCGCTTTATA 
GATTTTGAAT TTTCCGAACC AACAATTATT CTTTTCGAAA GAGAAAACTT CAAAGGAAAA 
AAGATAGAAC TGAACGCAGA AACAGTCAAT CTCCGATCCT TGGGGTTCAA CACTCAGATC 
CGCTCTGTGC GGGTCGTTGG TGGCATATGG GTTACCTATG AATATGGCAA TTACAGAGGC 
CGGCAGTTCC TGCTGTCTCC TGCAGAAGTA CCAAACTGGT ATGAGTTCAG TGCCTGTCGC 
CAAATAGGCT CCCTCCGGCC CTTTGCTCAG AAACGAATTT ACTTCAGACT TCGAAACAAA 
GCAACAGGAC TATTTATGTC AACCAACGGG AACTTGGAAG ACCTGAAGCT TCTCCGAGTC 
CAGGTCATGG AAGATGTTGG TGCTGATGAT CAGATCTGGA TCTATCAAGA AGGGTGCATC 
AAATGCAGGA TAGCAGAAGA CTGCTGCCTG ACGATCGTGG GCAGCCTCGT CACGTCTGGC 
TCCAAACTAG GACTGGCCCT CGATCAAAAT GTCGACAGTC AGTTCTGGTG CATGAAGTCC 
GACGGCAGGA TCTACAGCAA GATGAAACCA AATTTAGTGT TGGATGTGAA AGGGGGTGCG 
CAGTATGATC AAAATCACAT CATCCTCAAC ACAGTGAGCA CAGAGAAGTT AACACAAGTG 
TGGGAAGCCA TGGTCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aim1 ( NM_001368305.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355234.1 Mus musculus crystallin beta-gamma domain containing 1 (Crybg1), transcript variant 1, mRNA.

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CloneID	OMu43695
Clone ID Related Accession (Same CDS sequence)	NM_001368305.1 , XM_006512498.3
Accession Version	NM_001368305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	beta/gamma crystallin domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153863.6, AC153534.2 and AC158637.3. On Feb 5, 2019 this sequence version replaced XM_006512498.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGCCGTTGT CGCCACCATC GCAGGGCGAC CCCAGGGAGG CCAGCCCGTC CAAGGCCCCC 
AAGAAACACC CCACCTTCCA TATCTGGCGC TCCAAGAAGA AGCAGTCTCC ATCGTCCAGC 
TGTGGGGTGT TTATCCCTCA CCCGCCCGGG GCTTTCGGGG AGACCAGAGC TTTCCTTGTA 
CCCGATGGAA GCCAAGGGGA GCCCCAGGAG TTAACACTGC ATGGTGGTGA ACTACAGTTA 
TCTGACACCC CCAGTGAGGT GCCTGGCCCT TCATCTACGG AGTCTGCAAT GTTAAAAAAG 
TCCAGAGCAC AACCACCAGA AGACACCAGA AGGAAGCCAG TGTTGGAAAA ACTTGGCACT 
CTTTTTACGG CTGGAAGGAG AAGGAACACC AGGAACGGAT TAGAGAGTCC CACCAACTCA 
AATAGCAAAT CGGCCTCTGC TAAAGTCCCA AAAACAGGGA GTGAGAAAAC TAAGGCTCAG 
GACAACCAAC TAAAGGCAAC CGACGCTGGC GAGGCGGGCC CCAGGCAGGA GGCTGAGGGC 
CAGCCCGTTG AGAGCTGTGT TCAGGCAACC CCCTGCGACG CCGAACAGTC ACCTTGCTCC 
AGCATCAATG TAGGGGTTGT GCAGCAGTGC CACAGCAATG ATTCAGCCCA ATTAGAACCT 
CTACAGGCAG AGGGAGAGAC TTTCCCAGAT GCCACCACCG CTGCCAAAGA GCTGCTGCAT 
TCCTCACCAG GGAATCGCTC CAGGCAGGAG AGCGCAGAGA CGCCCGCCCG CAGTCCGGGG 
GAGGAAGCGT TGCCTGGCGC TGGCCTCCAA CCAGAGACAG CGAGAGGTGT GTCGGGGTCG 
CCGCCAGAGG AGCACCTCGT GGGAGGAGTA GGAGAGGCCC CCGACGGAGC CCCCGGCGTG 
GGTGCCGAAG CGGGCTCTCT GGGGGCCCCG GGGGACGCGG TTCCTCCAGG TGAGCCTCCC 
GCCCAGGACG CAGAGACCAG CCGCACAGCC GGGTCGGTCG ACCGCGCGCA TCCCACCAAA 
GTGCTAACGT TGGACATCTA CTTGAGCAAA ACTGAGGTGG CTCAGGTGGA CGAGCCGGCC 
TCGATTGCTC CGGGGACAGA CGACTGCGAG GATTCGGACG ACATGGAAAA GAGGTCAAGC 
GGCCGCCGGT CTGGTCGGCG GAGGAAGTCG CAGAAATCCA CCGACTCCCC GGGTGCGGAT 
GCGGTGGCTC CCGAGAGTGC GGCCAAGGAC GAGGCGGTGT TCGACGACGA GGTGGCGCCA 
GACGCTGCGG CTGAGAACTG CTTGGCGGAA AGGAAAGTGA AATCGGTACA AGCGGTTCCC 
GACGGAGGCG CAGCCCCGGT CGCCTGCCCG GAGCCAAAGG CCAGCTCCTG CCCCAGAGGG 
CAACCCCGAG GGGAACCGGA CCGCAGCAAG CCCCTGTCGC CCACCAAGCG GAAGGGTCGC 
AGCCGTGTCC CCGAGGCGGT GCCCAGCTCG CCCGGCAGTG GCTCGCGCGC TCCCGCCAAG 
GAGTCCCCAC CCAAGAGGGC GCCCGCTGCG GCGGACAGCG GCGAGGAGGC GGGCCGCGTC 
GTCCCGCGCG AGCTCACCGT CAAAAGCAGC TCGTTGCTGC CCGAGATCAA GCCAGAGCAC 
AAGAGGGGTC CTCTGCCCCA CCACTTTGAT AGCCGGGGCG ACGGGGGTCG CAGCAGGGGA 
ACAGGAGCGT CCGACGCCGA CGGCCTGAAG CCCAGGAACC AGTTCGGTGT GGGCAGGTCT 
ACTGTGACCA CCAAAGTGAC CCTCCCCGCC AGACCCAAAC ATGTGGAACT AAATCTGAAA 
ACTCCGAAGA ATCCTGACAG TTTGGGAAAT GAGCACAACT CATTTAGCCA GCCAGTTCAT 
AAGGGGAACA CAGCCAACAA AATCTCCTTA TTTGAAAACA AGCGGGCTAA CAGTAGCCCC 
CGCCACCCCG ACGTCCGTAA CACAAGGAAC AATCCTCCCT CTAGTAAGAC ATTTGTTGGG 
AGGGCGAAGC TGAACTTAGC CAAAAAGGCC AAAGAAATGG AGCAACCCGA AAAGAAAGCT 
GTGTCAACCA GCCACCAGAA TGGCCTGGTG GTCAAAGAGC CATCTGCAGA AGGGAAAGGC 
ACAGCAGTCC CTGTCCCCAG AGAAGAGATT CGTCCAGCCA CCAGAGGAGG GGAAGGAGAG 
TCCACCAAGG ATCAGGTCCT CCGCCCTGAG CCTGAGCAAA GTGATAAGCT GGATGTACAA 
ACAGACACTG CCTATCCTTC CCACCCAGGG CCCACTGCTC TGGTTCCTGT GAAGGACTCT 
GAGCTCTCAG GAGAGGGCCA GCCAGAGGCT GCTGACAGCA AAGGCCCCGT CCTTAACAAC 
ACAAGTGATG TTGCACAGGA CATCGCTGCC ATTTGTGATG CCAGAGAGGC TCCCCCAAAG 
CCACAGGATG CATTTTCTGA CTCACAGCCA GCTTCAGCGG CTGAGTCAGC TAGGGAGTCA 
TCTCTCCCGC CTTCTGCACC CATTTCTGTG GACGCTCCCC AAGATGGCTG TGTTCAAGTT 
CCCATATGCA GTGTTCCCTG CACTGCTCTA GAAGTGTCAG AAAACCACAA TGGGTGTGTT 
TCGCCAGTCT CTCCTCACAC TGAGAAAACT CCCATCTCCA AACCTGGAGT GGGAGAAGCC 
AGTCCCCCTT CCACTCCAGA GCACAGCCCA GAAACTATGG GGAAAGACTG TCCCTCCAAA 
GTCCTCGTCC AGGTCCGGTC CTTTGTGCTT CCCGTGGAGA ATCCTCAGGA TGTGAGCTCT 
CAGGTCATCT CAGAGAGCTC TGAAGTTAGA GAGGCACAGC TGCCAAGCTG TCACAGTAAT 
GAGCCTGAAG TGGTGTCTGT CACCACTTGT GTTCCGCAGC ACGAGGATGC ACCAGACACA 
TCACCCAAGG ACATCCATCA CAGAGAAGGA CATGTGGCAC AGTCTCGACC ACACATCACA 
CAGTCAGGAC CTGAGAAGGC TCCGCCCATC CAGAGCAGCA GTGGGATGCC CCAGACAGCG 
GAGTTCATCC TCACCCACTC TCCCAGCAGC AGAAGTCATG CAGAAGGCCC CCAAAGGCCA 
GGTCAAGATG CGACCAGCCC ACTTACCCCT ACCAGGCTTT TGAACATTTC TGCTGGTAGT 
GACAATAGTG TCTTGGATTC TTCTTCTGAT ATGGAAAAAT TCACTGAAAT TATTAAAAAG 
ATGGACAGTG CAGTATGTGT GCCCCAGAAA AAGAAGAAGG CCCGGGTCCC AAACTCTCCA 
GCCCCTCACT TCGCTATGCC TCCCATTCAC GAGGACAGTT TGGAAAAGGT GTTTGATCCC 
AACGTGTTCA CCATTGGTTT AGGGAAGAAG AAGGAAAGCC TGGCAGAGGT GTCACCAGCT 
TTGCACTTGT TGCAGAACCA CGACCCCATA TCCAAACTGA GAGCCAAGCG TGCATCCACC 
GAGCAGAGCA TCGTCTTCAA GTCCTTGCAC ACTAACAGTA ATGGGAAGAG CGAGCCACAG 
ACCACTTCAG AAGCAAGTGA CAAGGAGAAC AGGGACATCA ACAGCGGCGG CGTTAAGAGG 
TCACGGCTAG AAAACAGCAC GCTTTTCTCA AGCATGCTTT CTTCTCTGCC ACAAGACAAA 
GTCTTTTCTC CCTCCGTGAC GTCAGTCAAC ACAATGAGCA CATCTTTTAG CACTTCTCAG 
AACAGCTCCG TATCTCAGTC ATCAGTGGTA CAGCCCAGGA AGGAGGGTGG CCTGCCCTGT 
GGCTCAGACA AAGAACAGCC ACACCTTGCA CCCCACAACT CCTTAAAGGT CTTCAATTTC 
AATTCATCAA ATACATCTCG TCTGGGTTCC AGCCACATGG AAAAGTACCC ACAAAAAGAG 
GGAAGCAAGG AAGATGTGAA TTCTCAGGGC AACCTACAAT TGCCAGAAAA CAACCTTTCA 
GACTTACCCA AACTGAAGAA CAGCGATGGC GTGGAAGGTA TTCTGAAGTC AAACCTGCCG 
AATGTTGGGA GCGTGGACGC GGACTTCCTG GGCCTTTTCA AAGCAAGCCG GTATGACCCA 
GGCCATTCCT TTTCTGGAAT GTCCTTATCA GACTCTATGA CTCTTAGAGG AAGTATGCAC 
AGCAAACTCA ACCCCCGGCC TGGCAAGGTA GTGATATTCA GTGAGCCCGA CGTCTCTGAG 
GAGTGCATTG AGGTGTTTGG GGACATCCAG GACTGCAGCT CCTGGCGCCT CTCTCCAGTG 
ATAGTAGTGA AAGTTGTCCG AGGATGTTGG ATTTTGTATG AGAAAGCCAA TTTTGAAGGG 
CATTCTCTTG CTTTAGAAGA AGGTGAACTG GAACTCTCTA GTATCTGGGG AACAGAGGAA 
ATGTTGGATG AAGAAGCAGA GTCTGATAAA CCTGTAGTGA TTGGTTCCAT CAGACACGTG 
GTTCAGGATT ACAGAATTAG TCAGATCGAC TTGTTTACTG AGCCTGAAGG GTTAGGACTC 
CTGAACTCGT ACTTTGATGA CACTGAGGAA ATGCAGGGCT TCCCTGTGAT GCAGAAGACG 
TGTTCCATCA AAGTGCACTG GGGCACATGG CTGATCTATG AAGAGCCTGG ATTCCAAGGT 
GTCCCCTTCA TTCTGGAGCC CGGAGAATAC CCTGACCTAT CCTTCTGGGA TACCGAGGAA 
GCGTACATCG GATCCATGCG GCCTCTGAAA ATGGGTGGCC GTAAAGTTGA ATTCCCTACA 
GATCCAAAGG TAGTTATTTA TGAAAAGCCT TCCTTTGAAG GAAGGTGTAT GGAGCTAGAA 
ACAGAAATGA GTAGTTTTAT CATGGAAGGA GGTGAGACAG AAGAAACAAC TGGAAACAAC 
AACTTGCCCT TTACATCCGT GGGGTCTATG AAAGTCCTAA GAGGCGTCTG GGTTGCTTAT 
GAGAAGTGTG GCTTCACTGG CCACCAGTAT TTGCTGGAGG AGGGAGAATA CAGGGACTGG 
AACGCCTGGG GAGGCTACAA TGGAGAGCTC CAGTCTCTGC GGCCTATATT AAGTGATTTT 
TCAAATGCGC ACATGATAAT GTACAGTGAG AAGAACTTTG GCTCCAAAGG TTCCAGTATT 
GATGTGTTGG GAATTGTTGC CAATTTGAAG GAGACCGGCT ACGGAGTGAA GACACAGTCA 
ATCAATGTAC TGAGTGGCGT GTGGGTAGCC TATGAAAACC CGGACTTCAC GGGGGAGCAG 
TATGTCCTGG ATAAAGGCTT CTACACCAGT TTTGAGGATT GGGGAGGCAA AAATTGTAAG 
ATCTCTTCCG TCCAACCCAT ATGCCTGGAT TCTTTCACTG GCCCCAGGAG ACGAAATCAG 
ATTCACTTGT TTTCAGAACC ACAGTTCCAA GGTCAAAGTC AAAGTTTTGA AGAAACAATA 
GATCAGATTG ACGACTCCTT TCAGACCAAG TCTTGCAGAG TTTTGGGAGG CAGCTGGGTT 
GCTTATGATG GAGAAAATTT CTCTGGCAAT CAGTATGTAC TGGAAGAAGG CCACTATCCT 
TGTCTCTCGG CAATGGGATG CCTGCCTGGA GCAACCCTGA AGTCTCTTCG CTTTATAGAT 
TTTGAATTTT CCGAACCAAC AATTATTCTT TTCGAAAGAG AAAACTTCAA AGGAAAAAAG 
ATAGAACTGA ACGCAGAAAC AGTCAATCTC CGATCCTTGG GGTTCAACAC TCAGATCCGC 
TCTGTGCGGG TCGTTGGTGG CATATGGGTT ACCTATGAAT ATGGCAATTA CAGAGGCCGG 
CAGTTCCTGC TGTCTCCTGC AGAAGTACCA AACTGGTATG AGTTCAGTGC CTGTCGCCAA 
ATAGGCTCCC TCCGGCCCTT TGCTCAGAAA CGAATTTACT TCAGACTTCG AAACAAAGCA 
ACAGGACTAT TTATGTCAAC CAACGGGAAC TTGGAAGACC TGAAGCTTCT CCGAGTCCAG 
GTCATGGAAG ATGTTGGTGC TGATGATCAG ATCTGGATCT ATCAAGAAGG GTGCATCAAA 
TGCAGGATAG CAGAAGACTG CTGCCTGACG ATCGTGGGCA GCCTCGTCAC GTCTGGCTCC 
AAACTAGGAC TGGCCCTCGA TCAAAATGTC GACAGTCAGT TCTGGTGCAT GAAGTCCGAC 
GGCAGGATCT ACAGCAAGAT GAAACCAAAT TTAGTGTTGG ATGTGAAAGG GGGTGCGCAG 
TATGATCAAA ATCACATCAT CCTCAACACA GTGAGCACAG AGAAGTTAAC ACAAGTGTGG 
GAAGCCATGG TCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355234.1
CDS	70..6267
Translation	MPLSPPSQGDPREASPSKAPKKHPTFHIWRSKKKQSPSSSCGVFIPHPPGAFGETRAFLVPDGSQGEPQELTLHGGELQLSDTPSEVPGPSSTESAMLKKSRAQPPEDTRRKPVLEKLGTLFTAGRRRNTRNGLESPTNSNSKSASAKVPKTGSEKTKAQDNQLKATDAGEAGPRQEAEGQPVESCVQATPCDAEQSPCSSINVGVVQQCHSNDSAQLEPLQAEGETFPDATTAAKELLHSSPGNRSRQESAETPARSPGEEALPGAGLQPETARGVSGSPPEEHLVGGVGEAPDGAPGVGAEAGSLGAPGDAVPPGEPPAQDAETSRTAGSVDRAHPTKVLTLDIYLSKTEVAQVDEPASIAPGTDDCEDSDDMEKRSSGRRSGRRRKSQKSTDSPGADAVAPESAAKDEAVFDDEVAPDAAAENCLAERKVKSVQAVPDGGAAPVACPEPKASSCPRGQPRGEPDRSKPLSPTKRKGRSRVPEAVPSSPGSGSRAPAKESPPKRAPAAADSGEEAGRVVPRELTVKSSSLLPEIKPEHKRGPLPHHFDSRGDGGRSRGTGASDADGLKPRNQFGVGRSTVTTKVTLPARPKHVELNLKTPKNPDSLGNEHNSFSQPVHKGNTANKISLFENKRANSSPRHPDVRNTRNNPPSSKTFVGRAKLNLAKKAKEMEQPEKKAVSTSHQNGLVVKEPSAEGKGTAVPVPREEIRPATRGGEGESTKDQVLRPEPEQSDKLDVQTDTAYPSHPGPTALVPVKDSELSGEGQPEAADSKGPVLNNTSDVAQDIAAICDAREAPPKPQDAFSDSQPASAAESARESSLPPSAPISVDAPQDGCVQVPICSVPCTALEVSENHNGCVSPVSPHTEKTPISKPGVGEASPPSTPEHSPETMGKDCPSKVLVQVRSFVLPVENPQDVSSQVISESSEVREAQLPSCHSNEPEVVSVTTCVPQHEDAPDTSPKDIHHREGHVAQSRPHITQSGPEKAPPIQSSSGMPQTAEFILTHSPSSRSHAEGPQRPGQDATSPLTPTRLLNISAGSDNSVLDSSSDMEKFTEIIKKMDSAVCVPQKKKKARVPNSPAPHFAMPPIHEDSLEKVFDPNVFTIGLGKKKESLAEVSPALHLLQNHDPISKLRAKRASTEQSIVFKSLHTNSNGKSEPQTTSEASDKENRDINSGGVKRSRLENSTLFSSMLSSLPQDKVFSPSVTSVNTMSTSFSTSQNSSVSQSSVVQPRKEGGLPCGSDKEQPHLAPHNSLKVFNFNSSNTSRLGSSHMEKYPQKEGSKEDVNSQGNLQLPENNLSDLPKLKNSDGVEGILKSNLPNVGSVDADFLGLFKASRYDPGHSFSGMSLSDSMTLRGSMHSKLNPRPGKVVIFSEPDVSEECIEVFGDIQDCSSWRLSPVIVVKVVRGCWILYEKANFEGHSLALEEGELELSSIWGTEEMLDEEAESDKPVVIGSIRHVVQDYRISQIDLFTEPEGLGLLNSYFDDTEEMQGFPVMQKTCSIKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVIYEKPSFEGRCMELETEMSSFIMEGGETEETTGNNNLPFTSVGSMKVLRGVWVAYEKCGFTGHQYLLEEGEYRDWNAWGGYNGELQSLRPILSDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYVLDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGQSQSFEETIDQIDDSFQTKSCRVLGGSWVAYDGENFSGNQYVLEEGHYPCLSAMGCLPGATLKSLRFIDFEFSEPTIILFERENFKGKKIELNAETVNLRSLGFNTQIRSVRVVGGIWVTYEYGNYRGRQFLLSPAEVPNWYEFSACRQIGSLRPFAQKRIYFRLRNKATGLFMSTNGNLEDLKLLRVQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNVDSQFWCMKSDGRIYSKMKPNLVLDVKGGAQYDQNHIILNTVSTEKLTQVWEAMVL

Target ORF information:

RefSeq Version	NM_001368305.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crystallin beta-gamma domain containing 1 (Crybg1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368305.1

ORF Insert Sequence:

ATGCCGTTGT CGCCACCATC GCAGGGCGAC CCCAGGGAGG CCAGCCCGTC CAAGGCCCCC 
AAGAAACACC CCACCTTCCA TATCTGGCGC TCCAAGAAGA AGCAGTCTCC ATCGTCCAGC 
TGTGGGGTGT TTATCCCTCA CCCGCCCGGG GCTTTCGGGG AGACCAGAGC TTTCCTTGTA 
CCCGATGGAA GCCAAGGGGA GCCCCAGGAG TTAACACTGC ATGGTGGTGA ACTACAGTTA 
TCTGACACCC CCAGTGAGGT GCCTGGCCCT TCATCTACGG AGTCTGCAAT GTTAAAAAAG 
TCCAGAGCAC AACCACCAGA AGACACCAGA AGGAAGCCAG TGTTGGAAAA ACTTGGCACT 
CTTTTTACGG CTGGAAGGAG AAGGAACACC AGGAACGGAT TAGAGAGTCC CACCAACTCA 
AATAGCAAAT CGGCCTCTGC TAAAGTCCCA AAAACAGGGA GTGAGAAAAC TAAGGCTCAG 
GACAACCAAC TAAAGGCAAC CGACGCTGGC GAGGCGGGCC CCAGGCAGGA GGCTGAGGGC 
CAGCCCGTTG AGAGCTGTGT TCAGGCAACC CCCTGCGACG CCGAACAGTC ACCTTGCTCC 
AGCATCAATG TAGGGGTTGT GCAGCAGTGC CACAGCAATG ATTCAGCCCA ATTAGAACCT 
CTACAGGCAG AGGGAGAGAC TTTCCCAGAT GCCACCACCG CTGCCAAAGA GCTGCTGCAT 
TCCTCACCAG GGAATCGCTC CAGGCAGGAG AGCGCAGAGA CGCCCGCCCG CAGTCCGGGG 
GAGGAAGCGT TGCCTGGCGC TGGCCTCCAA CCAGAGACAG CGAGAGGTGT GTCGGGGTCG 
CCGCCAGAGG AGCACCTCGT GGGAGGAGTA GGAGAGGCCC CCGACGGAGC CCCCGGCGTG 
GGTGCCGAAG CGGGCTCTCT GGGGGCCCCG GGGGACGCGG TTCCTCCAGG TGAGCCTCCC 
GCCCAGGACG CAGAGACCAG CCGCACAGCC GGGTCGGTCG ACCGCGCGCA TCCCACCAAA 
GTGCTAACGT TGGACATCTA CTTGAGCAAA ACTGAGGTGG CTCAGGTGGA CGAGCCGGCC 
TCGATTGCTC CGGGGACAGA CGACTGCGAG GATTCGGACG ACATGGAAAA GAGGTCAAGC 
GGCCGCCGGT CTGGTCGGCG GAGGAAGTCG CAGAAATCCA CCGACTCCCC GGGTGCGGAT 
GCGGTGGCTC CCGAGAGTGC GGCCAAGGAC GAGGCGGTGT TCGACGACGA GGTGGCGCCA 
GACGCTGCGG CTGAGAACTG CTTGGCGGAA AGGAAAGTGA AATCGGTACA AGCGGTTCCC 
GACGGAGGCG CAGCCCCGGT CGCCTGCCCG GAGCCAAAGG CCAGCTCCTG CCCCAGAGGG 
CAACCCCGAG GGGAACCGGA CCGCAGCAAG CCCCTGTCGC CCACCAAGCG GAAGGGTCGC 
AGCCGTGTCC CCGAGGCGGT GCCCAGCTCG CCCGGCAGTG GCTCGCGCGC TCCCGCCAAG 
GAGTCCCCAC CCAAGAGGGC GCCCGCTGCG GCGGACAGCG GCGAGGAGGC GGGCCGCGTC 
GTCCCGCGCG AGCTCACCGT CAAAAGCAGC TCGTTGCTGC CCGAGATCAA GCCAGAGCAC 
AAGAGGGGTC CTCTGCCCCA CCACTTTGAT AGCCGGGGCG ACGGGGGTCG CAGCAGGGGA 
ACAGGAGCGT CCGACGCCGA CGGCCTGAAG CCCAGGAACC AGTTCGGTGT GGGCAGGTCT 
ACTGTGACCA CCAAAGTGAC CCTCCCCGCC AGACCCAAAC ATGTGGAACT AAATCTGAAA 
ACTCCGAAGA ATCCTGACAG TTTGGGAAAT GAGCACAACT CATTTAGCCA GCCAGTTCAT 
AAGGGGAACA CAGCCAACAA AATCTCCTTA TTTGAAAACA AGCGGGCTAA CAGTAGCCCC 
CGCCACCCCG ACGTCCGTAA CACAAGGAAC AATCCTCCCT CTAGTAAGAC ATTTGTTGGG 
AGGGCGAAGC TGAACTTAGC CAAAAAGGCC AAAGAAATGG AGCAACCCGA AAAGAAAGCT 
GTGTCAACCA GCCACCAGAA TGGCCTGGTG GTCAAAGAGC CATCTGCAGA AGGGAAAGGC 
ACAGCAGTCC CTGTCCCCAG AGAAGAGATT CGTCCAGCCA CCAGAGGAGG GGAAGGAGAG 
TCCACCAAGG ATCAGGTCCT CCGCCCTGAG CCTGAGCAAA GTGATAAGCT GGATGTACAA 
ACAGACACTG CCTATCCTTC CCACCCAGGG CCCACTGCTC TGGTTCCTGT GAAGGACTCT 
GAGCTCTCAG GAGAGGGCCA GCCAGAGGCT GCTGACAGCA AAGGCCCCGT CCTTAACAAC 
ACAAGTGATG TTGCACAGGA CATCGCTGCC ATTTGTGATG CCAGAGAGGC TCCCCCAAAG 
CCACAGGATG CATTTTCTGA CTCACAGCCA GCTTCAGCGG CTGAGTCAGC TAGGGAGTCA 
TCTCTCCCGC CTTCTGCACC CATTTCTGTG GACGCTCCCC AAGATGGCTG TGTTCAAGTT 
CCCATATGCA GTGTTCCCTG CACTGCTCTA GAAGTGTCAG AAAACCACAA TGGGTGTGTT 
TCGCCAGTCT CTCCTCACAC TGAGAAAACT CCCATCTCCA AACCTGGAGT GGGAGAAGCC 
AGTCCCCCTT CCACTCCAGA GCACAGCCCA GAAACTATGG GGAAAGACTG TCCCTCCAAA 
GTCCTCGTCC AGGTCCGGTC CTTTGTGCTT CCCGTGGAGA ATCCTCAGGA TGTGAGCTCT 
CAGGTCATCT CAGAGAGCTC TGAAGTTAGA GAGGCACAGC TGCCAAGCTG TCACAGTAAT 
GAGCCTGAAG TGGTGTCTGT CACCACTTGT GTTCCGCAGC ACGAGGATGC ACCAGACACA 
TCACCCAAGG ACATCCATCA CAGAGAAGGA CATGTGGCAC AGTCTCGACC ACACATCACA 
CAGTCAGGAC CTGAGAAGGC TCCGCCCATC CAGAGCAGCA GTGGGATGCC CCAGACAGCG 
GAGTTCATCC TCACCCACTC TCCCAGCAGC AGAAGTCATG CAGAAGGCCC CCAAAGGCCA 
GGTCAAGATG CGACCAGCCC ACTTACCCCT ACCAGGCTTT TGAACATTTC TGCTGGTAGT 
GACAATAGTG TCTTGGATTC TTCTTCTGAT ATGGAAAAAT TCACTGAAAT TATTAAAAAG 
ATGGACAGTG CAGTATGTGT GCCCCAGAAA AAGAAGAAGG CCCGGGTCCC AAACTCTCCA 
GCCCCTCACT TCGCTATGCC TCCCATTCAC GAGGACAGTT TGGAAAAGGT GTTTGATCCC 
AACGTGTTCA CCATTGGTTT AGGGAAGAAG AAGGAAAGCC TGGCAGAGGT GTCACCAGCT 
TTGCACTTGT TGCAGAACCA CGACCCCATA TCCAAACTGA GAGCCAAGCG TGCATCCACC 
GAGCAGAGCA TCGTCTTCAA GTCCTTGCAC ACTAACAGTA ATGGGAAGAG CGAGCCACAG 
ACCACTTCAG AAGCAAGTGA CAAGGAGAAC AGGGACATCA ACAGCGGCGG CGTTAAGAGG 
TCACGGCTAG AAAACAGCAC GCTTTTCTCA AGCATGCTTT CTTCTCTGCC ACAAGACAAA 
GTCTTTTCTC CCTCCGTGAC GTCAGTCAAC ACAATGAGCA CATCTTTTAG CACTTCTCAG 
AACAGCTCCG TATCTCAGTC ATCAGTGGTA CAGCCCAGGA AGGAGGGTGG CCTGCCCTGT 
GGCTCAGACA AAGAACAGCC ACACCTTGCA CCCCACAACT CCTTAAAGGT CTTCAATTTC 
AATTCATCAA ATACATCTCG TCTGGGTTCC AGCCACATGG AAAAGTACCC ACAAAAAGAG 
GGAAGCAAGG AAGATGTGAA TTCTCAGGGC AACCTACAAT TGCCAGAAAA CAACCTTTCA 
GACTTACCCA AACTGAAGAA CAGCGATGGC GTGGAAGGTA TTCTGAAGTC AAACCTGCCG 
AATGTTGGGA GCGTGGACGC GGACTTCCTG GGCCTTTTCA AAGCAAGCCG GTATGACCCA 
GGCCATTCCT TTTCTGGAAT GTCCTTATCA GACTCTATGA CTCTTAGAGG AAGTATGCAC 
AGCAAACTCA ACCCCCGGCC TGGCAAGGTA GTGATATTCA GTGAGCCCGA CGTCTCTGAG 
GAGTGCATTG AGGTGTTTGG GGACATCCAG GACTGCAGCT CCTGGCGCCT CTCTCCAGTG 
ATAGTAGTGA AAGTTGTCCG AGGATGTTGG ATTTTGTATG AGAAAGCCAA TTTTGAAGGG 
CATTCTCTTG CTTTAGAAGA AGGTGAACTG GAACTCTCTA GTATCTGGGG AACAGAGGAA 
ATGTTGGATG AAGAAGCAGA GTCTGATAAA CCTGTAGTGA TTGGTTCCAT CAGACACGTG 
GTTCAGGATT ACAGAATTAG TCAGATCGAC TTGTTTACTG AGCCTGAAGG GTTAGGACTC 
CTGAACTCGT ACTTTGATGA CACTGAGGAA ATGCAGGGCT TCCCTGTGAT GCAGAAGACG 
TGTTCCATCA AAGTGCACTG GGGCACATGG CTGATCTATG AAGAGCCTGG ATTCCAAGGT 
GTCCCCTTCA TTCTGGAGCC CGGAGAATAC CCTGACCTAT CCTTCTGGGA TACCGAGGAA 
GCGTACATCG GATCCATGCG GCCTCTGAAA ATGGGTGGCC GTAAAGTTGA ATTCCCTACA 
GATCCAAAGG TAGTTATTTA TGAAAAGCCT TCCTTTGAAG GAAGGTGTAT GGAGCTAGAA 
ACAGAAATGA GTAGTTTTAT CATGGAAGGA GGTGAGACAG AAGAAACAAC TGGAAACAAC 
AACTTGCCCT TTACATCCGT GGGGTCTATG AAAGTCCTAA GAGGCGTCTG GGTTGCTTAT 
GAGAAGTGTG GCTTCACTGG CCACCAGTAT TTGCTGGAGG AGGGAGAATA CAGGGACTGG 
AACGCCTGGG GAGGCTACAA TGGAGAGCTC CAGTCTCTGC GGCCTATATT AAGTGATTTT 
TCAAATGCGC ACATGATAAT GTACAGTGAG AAGAACTTTG GCTCCAAAGG TTCCAGTATT 
GATGTGTTGG GAATTGTTGC CAATTTGAAG GAGACCGGCT ACGGAGTGAA GACACAGTCA 
ATCAATGTAC TGAGTGGCGT GTGGGTAGCC TATGAAAACC CGGACTTCAC GGGGGAGCAG 
TATGTCCTGG ATAAAGGCTT CTACACCAGT TTTGAGGATT GGGGAGGCAA AAATTGTAAG 
ATCTCTTCCG TCCAACCCAT ATGCCTGGAT TCTTTCACTG GCCCCAGGAG ACGAAATCAG 
ATTCACTTGT TTTCAGAACC ACAGTTCCAA GGTCAAAGTC AAAGTTTTGA AGAAACAATA 
GATCAGATTG ACGACTCCTT TCAGACCAAG TCTTGCAGAG TTTTGGGAGG CAGCTGGGTT 
GCTTATGATG GAGAAAATTT CTCTGGCAAT CAGTATGTAC TGGAAGAAGG CCACTATCCT 
TGTCTCTCGG CAATGGGATG CCTGCCTGGA GCAACCCTGA AGTCTCTTCG CTTTATAGAT 
TTTGAATTTT CCGAACCAAC AATTATTCTT TTCGAAAGAG AAAACTTCAA AGGAAAAAAG 
ATAGAACTGA ACGCAGAAAC AGTCAATCTC CGATCCTTGG GGTTCAACAC TCAGATCCGC 
TCTGTGCGGG TCGTTGGTGG CATATGGGTT ACCTATGAAT ATGGCAATTA CAGAGGCCGG 
CAGTTCCTGC TGTCTCCTGC AGAAGTACCA AACTGGTATG AGTTCAGTGC CTGTCGCCAA 
ATAGGCTCCC TCCGGCCCTT TGCTCAGAAA CGAATTTACT TCAGACTTCG AAACAAAGCA 
ACAGGACTAT TTATGTCAAC CAACGGGAAC TTGGAAGACC TGAAGCTTCT CCGAGTCCAG 
GTCATGGAAG ATGTTGGTGC TGATGATCAG ATCTGGATCT ATCAAGAAGG GTGCATCAAA 
TGCAGGATAG CAGAAGACTG CTGCCTGACG ATCGTGGGCA GCCTCGTCAC GTCTGGCTCC 
AAACTAGGAC TGGCCCTCGA TCAAAATGTC GACAGTCAGT TCTGGTGCAT GAAGTCCGAC 
GGCAGGATCT ACAGCAAGAT GAAACCAAAT TTAGTGTTGG ATGTGAAAGG GGGTGCGCAG 
TATGATCAAA ATCACATCAT CCTCAACACA GTGAGCACAG AGAAGTTAAC ACAAGTGTGG 
GAAGCCATGG TCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aim1 ( XM_006512498.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512561.1 Mus musculus absent in melanoma 1 (Aim1), transcript variant X1, mRNA.

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CloneID	OMu43695
Clone ID Related Accession (Same CDS sequence)	NM_001368305.1 , XM_006512498.3
Accession Version	XM_006512498.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	absent in melanoma 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTGT CGCCACCATC GCAGGGCGAC CCCAGGGAGG CCAGCCCGTC CAAGGCCCCC 
AAGAAACACC CCACCTTCCA TATCTGGCGC TCCAAGAAGA AGCAGTCTCC ATCGTCCAGC 
TGTGGGGTGT TTATCCCTCA CCCGCCCGGG GCTTTCGGGG AGACCAGAGC TTTCCTTGTA 
CCCGATGGAA GCCAAGGGGA GCCCCAGGAG TTAACACTGC ATGGTGGTGA ACTACAGTTA 
TCTGACACCC CCAGTGAGGT GCCTGGCCCT TCATCTACGG AGTCTGCAAT GTTAAAAAAG 
TCCAGAGCAC AACCACCAGA AGACACCAGA AGGAAGCCAG TGTTGGAAAA ACTTGGCACT 
CTTTTTACGG CTGGAAGGAG AAGGAACACC AGGAACGGAT TAGAGAGTCC CACCAACTCA 
AATAGCAAAT CGGCCTCTGC TAAAGTCCCA AAAACAGGGA GTGAGAAAAC TAAGGCTCAG 
GACAACCAAC TAAAGGCAAC CGACGCTGGC GAGGCGGGCC CCAGGCAGGA GGCTGAGGGC 
CAGCCCGTTG AGAGCTGTGT TCAGGCAACC CCCTGCGACG CCGAACAGTC ACCTTGCTCC 
AGCATCAATG TAGGGGTTGT GCAGCAGTGC CACAGCAATG ATTCAGCCCA ATTAGAACCT 
CTACAGGCAG AGGGAGAGAC TTTCCCAGAT GCCACCACCG CTGCCAAAGA GCTGCTGCAT 
TCCTCACCAG GGAATCGCTC CAGGCAGGAG AGCGCAGAGA CGCCCGCCCG CAGTCCGGGG 
GAGGAAGCGT TGCCTGGCGC TGGCCTCCAA CCAGAGACAG CGAGAGGTGT GTCGGGGTCG 
CCGCCAGAGG AGCACCTCGT GGGAGGAGTA GGAGAGGCCC CCGACGGAGC CCCCGGCGTG 
GGTGCCGAAG CGGGCTCTCT GGGGGCCCCG GGGGACGCGG TTCCTCCAGG TGAGCCTCCC 
GCCCAGGACG CAGAGACCAG CCGCACAGCC GGGTCGGTCG ACCGCGCGCA TCCCACCAAA 
GTGCTAACGT TGGACATCTA CTTGAGCAAA ACTGAGGTGG CTCAGGTGGA CGAGCCGGCC 
TCGATTGCTC CGGGGACAGA CGACTGCGAG GATTCGGACG ACATGGAAAA GAGGTCAAGC 
GGCCGCCGGT CTGGTCGGCG GAGGAAGTCG CAGAAATCCA CCGACTCCCC GGGTGCGGAT 
GCGGTGGCTC CCGAGAGTGC GGCCAAGGAC GAGGCGGTGT TCGACGACGA GGTGGCGCCA 
GACGCTGCGG CTGAGAACTG CTTGGCGGAA AGGAAAGTGA AATCGGTACA AGCGGTTCCC 
GACGGAGGCG CAGCCCCGGT CGCCTGCCCG GAGCCAAAGG CCAGCTCCTG CCCCAGAGGG 
CAACCCCGAG GGGAACCGGA CCGCAGCAAG CCCCTGTCGC CCACCAAGCG GAAGGGTCGC 
AGCCGTGTCC CCGAGGCGGT GCCCAGCTCG CCCGGCAGTG GCTCGCGCGC TCCCGCCAAG 
GAGTCCCCAC CCAAGAGGGC GCCCGCTGCG GCGGACAGCG GCGAGGAGGC GGGCCGCGTC 
GTCCCGCGCG AGCTCACCGT CAAAAGCAGC TCGTTGCTGC CCGAGATCAA GCCAGAGCAC 
AAGAGGGGTC CTCTGCCCCA CCACTTTGAT AGCCGGGGCG ACGGGGGTCG CAGCAGGGGA 
ACAGGAGCGT CCGACGCCGA CGGCCTGAAG CCCAGGAACC AGTTCGGTGT GGGCAGGTCT 
ACTGTGACCA CCAAAGTGAC CCTCCCCGCC AGACCCAAAC ATGTGGAACT AAATCTGAAA 
ACTCCGAAGA ATCCTGACAG TTTGGGAAAT GAGCACAACT CATTTAGCCA GCCAGTTCAT 
AAGGGGAACA CAGCCAACAA AATCTCCTTA TTTGAAAACA AGCGGGCTAA CAGTAGCCCC 
CGCCACCCCG ACGTCCGTAA CACAAGGAAC AATCCTCCCT CTAGTAAGAC ATTTGTTGGG 
AGGGCGAAGC TGAACTTAGC CAAAAAGGCC AAAGAAATGG AGCAACCCGA AAAGAAAGCT 
GTGTCAACCA GCCACCAGAA TGGCCTGGTG GTCAAAGAGC CATCTGCAGA AGGGAAAGGC 
ACAGCAGTCC CTGTCCCCAG AGAAGAGATT CGTCCAGCCA CCAGAGGAGG GGAAGGAGAG 
TCCACCAAGG ATCAGGTCCT CCGCCCTGAG CCTGAGCAAA GTGATAAGCT GGATGTACAA 
ACAGACACTG CCTATCCTTC CCACCCAGGG CCCACTGCTC TGGTTCCTGT GAAGGACTCT 
GAGCTCTCAG GAGAGGGCCA GCCAGAGGCT GCTGACAGCA AAGGCCCCGT CCTTAACAAC 
ACAAGTGATG TTGCACAGGA CATCGCTGCC ATTTGTGATG CCAGAGAGGC TCCCCCAAAG 
CCACAGGATG CATTTTCTGA CTCACAGCCA GCTTCAGCGG CTGAGTCAGC TAGGGAGTCA 
TCTCTCCCGC CTTCTGCACC CATTTCTGTG GACGCTCCCC AAGATGGCTG TGTTCAAGTT 
CCCATATGCA GTGTTCCCTG CACTGCTCTA GAAGTGTCAG AAAACCACAA TGGGTGTGTT 
TCGCCAGTCT CTCCTCACAC TGAGAAAACT CCCATCTCCA AACCTGGAGT GGGAGAAGCC 
AGTCCCCCTT CCACTCCAGA GCACAGCCCA GAAACTATGG GGAAAGACTG TCCCTCCAAA 
GTCCTCGTCC AGGTCCGGTC CTTTGTGCTT CCCGTGGAGA ATCCTCAGGA TGTGAGCTCT 
CAGGTCATCT CAGAGAGCTC TGAAGTTAGA GAGGCACAGC TGCCAAGCTG TCACAGTAAT 
GAGCCTGAAG TGGTGTCTGT CACCACTTGT GTTCCGCAGC ACGAGGATGC ACCAGACACA 
TCACCCAAGG ACATCCATCA CAGAGAAGGA CATGTGGCAC AGTCTCGACC ACACATCACA 
CAGTCAGGAC CTGAGAAGGC TCCGCCCATC CAGAGCAGCA GTGGGATGCC CCAGACAGCG 
GAGTTCATCC TCACCCACTC TCCCAGCAGC AGAAGTCATG CAGAAGGCCC CCAAAGGCCA 
GGTCAAGATG CGACCAGCCC ACTTACCCCT ACCAGGCTTT TGAACATTTC TGCTGGTAGT 
GACAATAGTG TCTTGGATTC TTCTTCTGAT ATGGAAAAAT TCACTGAAAT TATTAAAAAG 
ATGGACAGTG CAGTATGTGT GCCCCAGAAA AAGAAGAAGG CCCGGGTCCC AAACTCTCCA 
GCCCCTCACT TCGCTATGCC TCCCATTCAC GAGGACAGTT TGGAAAAGGT GTTTGATCCC 
AACGTGTTCA CCATTGGTTT AGGGAAGAAG AAGGAAAGCC TGGCAGAGGT GTCACCAGCT 
TTGCACTTGT TGCAGAACCA CGACCCCATA TCCAAACTGA GAGCCAAGCG TGCATCCACC 
GAGCAGAGCA TCGTCTTCAA GTCCTTGCAC ACTAACAGTA ATGGGAAGAG CGAGCCACAG 
ACCACTTCAG AAGCAAGTGA CAAGGAGAAC AGGGACATCA ACAGCGGCGG CGTTAAGAGG 
TCACGGCTAG AAAACAGCAC GCTTTTCTCA AGCATGCTTT CTTCTCTGCC ACAAGACAAA 
GTCTTTTCTC CCTCCGTGAC GTCAGTCAAC ACAATGAGCA CATCTTTTAG CACTTCTCAG 
AACAGCTCCG TATCTCAGTC ATCAGTGGTA CAGCCCAGGA AGGAGGGTGG CCTGCCCTGT 
GGCTCAGACA AAGAACAGCC ACACCTTGCA CCCCACAACT CCTTAAAGGT CTTCAATTTC 
AATTCATCAA ATACATCTCG TCTGGGTTCC AGCCACATGG AAAAGTACCC ACAAAAAGAG 
GGAAGCAAGG AAGATGTGAA TTCTCAGGGC AACCTACAAT TGCCAGAAAA CAACCTTTCA 
GACTTACCCA AACTGAAGAA CAGCGATGGC GTGGAAGGTA TTCTGAAGTC AAACCTGCCG 
AATGTTGGGA GCGTGGACGC GGACTTCCTG GGCCTTTTCA AAGCAAGCCG GTATGACCCA 
GGCCATTCCT TTTCTGGAAT GTCCTTATCA GACTCTATGA CTCTTAGAGG AAGTATGCAC 
AGCAAACTCA ACCCCCGGCC TGGCAAGGTA GTGATATTCA GTGAGCCCGA CGTCTCTGAG 
GAGTGCATTG AGGTGTTTGG GGACATCCAG GACTGCAGCT CCTGGCGCCT CTCTCCAGTG 
ATAGTAGTGA AAGTTGTCCG AGGATGTTGG ATTTTGTATG AGAAAGCCAA TTTTGAAGGG 
CATTCTCTTG CTTTAGAAGA AGGTGAACTG GAACTCTCTA GTATCTGGGG AACAGAGGAA 
ATGTTGGATG AAGAAGCAGA GTCTGATAAA CCTGTAGTGA TTGGTTCCAT CAGACACGTG 
GTTCAGGATT ACAGAATTAG TCAGATCGAC TTGTTTACTG AGCCTGAAGG GTTAGGACTC 
CTGAACTCGT ACTTTGATGA CACTGAGGAA ATGCAGGGCT TCCCTGTGAT GCAGAAGACG 
TGTTCCATCA AAGTGCACTG GGGCACATGG CTGATCTATG AAGAGCCTGG ATTCCAAGGT 
GTCCCCTTCA TTCTGGAGCC CGGAGAATAC CCTGACCTAT CCTTCTGGGA TACCGAGGAA 
GCGTACATCG GATCCATGCG GCCTCTGAAA ATGGGTGGCC GTAAAGTTGA ATTCCCTACA 
GATCCAAAGG TAGTTATTTA TGAAAAGCCT TCCTTTGAAG GAAGGTGTAT GGAGCTAGAA 
ACAGAAATGA GTAGTTTTAT CATGGAAGGA GGTGAGACAG AAGAAACAAC TGGAAACAAC 
AACTTGCCCT TTACATCCGT GGGGTCTATG AAAGTCCTAA GAGGCGTCTG GGTTGCTTAT 
GAGAAGTGTG GCTTCACTGG CCACCAGTAT TTGCTGGAGG AGGGAGAATA CAGGGACTGG 
AACGCCTGGG GAGGCTACAA TGGAGAGCTC CAGTCTCTGC GGCCTATATT AAGTGATTTT 
TCAAATGCGC ACATGATAAT GTACAGTGAG AAGAACTTTG GCTCCAAAGG TTCCAGTATT 
GATGTGTTGG GAATTGTTGC CAATTTGAAG GAGACCGGCT ACGGAGTGAA GACACAGTCA 
ATCAATGTAC TGAGTGGCGT GTGGGTAGCC TATGAAAACC CGGACTTCAC GGGGGAGCAG 
TATGTCCTGG ATAAAGGCTT CTACACCAGT TTTGAGGATT GGGGAGGCAA AAATTGTAAG 
ATCTCTTCCG TCCAACCCAT ATGCCTGGAT TCTTTCACTG GCCCCAGGAG ACGAAATCAG 
ATTCACTTGT TTTCAGAACC ACAGTTCCAA GGTCAAAGTC AAAGTTTTGA AGAAACAATA 
GATCAGATTG ACGACTCCTT TCAGACCAAG TCTTGCAGAG TTTTGGGAGG CAGCTGGGTT 
GCTTATGATG GAGAAAATTT CTCTGGCAAT CAGTATGTAC TGGAAGAAGG CCACTATCCT 
TGTCTCTCGG CAATGGGATG CCTGCCTGGA GCAACCCTGA AGTCTCTTCG CTTTATAGAT 
TTTGAATTTT CCGAACCAAC AATTATTCTT TTCGAAAGAG AAAACTTCAA AGGAAAAAAG 
ATAGAACTGA ACGCAGAAAC AGTCAATCTC CGATCCTTGG GGTTCAACAC TCAGATCCGC 
TCTGTGCGGG TCGTTGGTGG CATATGGGTT ACCTATGAAT ATGGCAATTA CAGAGGCCGG 
CAGTTCCTGC TGTCTCCTGC AGAAGTACCA AACTGGTATG AGTTCAGTGC CTGTCGCCAA 
ATAGGCTCCC TCCGGCCCTT TGCTCAGAAA CGAATTTACT TCAGACTTCG AAACAAAGCA 
ACAGGACTAT TTATGTCAAC CAACGGGAAC TTGGAAGACC TGAAGCTTCT CCGAGTCCAG 
GTCATGGAAG ATGTTGGTGC TGATGATCAG ATCTGGATCT ATCAAGAAGG GTGCATCAAA 
TGCAGGATAG CAGAAGACTG CTGCCTGACG ATCGTGGGCA GCCTCGTCAC GTCTGGCTCC 
AAACTAGGAC TGGCCCTCGA TCAAAATGTC GACAGTCAGT TCTGGTGCAT GAAGTCCGAC 
GGCAGGATCT ACAGCAAGAT GAAACCAAAT TTAGTGTTGG ATGTGAAAGG GGGTGCGCAG 
TATGATCAAA ATCACATCAT CCTCAACACA GTGAGCACAG AGAAGTTAAC ACAAGTGTGG 
GAAGCCATGG TCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512561.1
CDS	169..6366
Translation	MPLSPPSQGDPREASPSKAPKKHPTFHIWRSKKKQSPSSSCGVFIPHPPGAFGETRAFLVPDGSQGEPQELTLHGGELQLSDTPSEVPGPSSTESAMLKKSRAQPPEDTRRKPVLEKLGTLFTAGRRRNTRNGLESPTNSNSKSASAKVPKTGSEKTKAQDNQLKATDAGEAGPRQEAEGQPVESCVQATPCDAEQSPCSSINVGVVQQCHSNDSAQLEPLQAEGETFPDATTAAKELLHSSPGNRSRQESAETPARSPGEEALPGAGLQPETARGVSGSPPEEHLVGGVGEAPDGAPGVGAEAGSLGAPGDAVPPGEPPAQDAETSRTAGSVDRAHPTKVLTLDIYLSKTEVAQVDEPASIAPGTDDCEDSDDMEKRSSGRRSGRRRKSQKSTDSPGADAVAPESAAKDEAVFDDEVAPDAAAENCLAERKVKSVQAVPDGGAAPVACPEPKASSCPRGQPRGEPDRSKPLSPTKRKGRSRVPEAVPSSPGSGSRAPAKESPPKRAPAAADSGEEAGRVVPRELTVKSSSLLPEIKPEHKRGPLPHHFDSRGDGGRSRGTGASDADGLKPRNQFGVGRSTVTTKVTLPARPKHVELNLKTPKNPDSLGNEHNSFSQPVHKGNTANKISLFENKRANSSPRHPDVRNTRNNPPSSKTFVGRAKLNLAKKAKEMEQPEKKAVSTSHQNGLVVKEPSAEGKGTAVPVPREEIRPATRGGEGESTKDQVLRPEPEQSDKLDVQTDTAYPSHPGPTALVPVKDSELSGEGQPEAADSKGPVLNNTSDVAQDIAAICDAREAPPKPQDAFSDSQPASAAESARESSLPPSAPISVDAPQDGCVQVPICSVPCTALEVSENHNGCVSPVSPHTEKTPISKPGVGEASPPSTPEHSPETMGKDCPSKVLVQVRSFVLPVENPQDVSSQVISESSEVREAQLPSCHSNEPEVVSVTTCVPQHEDAPDTSPKDIHHREGHVAQSRPHITQSGPEKAPPIQSSSGMPQTAEFILTHSPSSRSHAEGPQRPGQDATSPLTPTRLLNISAGSDNSVLDSSSDMEKFTEIIKKMDSAVCVPQKKKKARVPNSPAPHFAMPPIHEDSLEKVFDPNVFTIGLGKKKESLAEVSPALHLLQNHDPISKLRAKRASTEQSIVFKSLHTNSNGKSEPQTTSEASDKENRDINSGGVKRSRLENSTLFSSMLSSLPQDKVFSPSVTSVNTMSTSFSTSQNSSVSQSSVVQPRKEGGLPCGSDKEQPHLAPHNSLKVFNFNSSNTSRLGSSHMEKYPQKEGSKEDVNSQGNLQLPENNLSDLPKLKNSDGVEGILKSNLPNVGSVDADFLGLFKASRYDPGHSFSGMSLSDSMTLRGSMHSKLNPRPGKVVIFSEPDVSEECIEVFGDIQDCSSWRLSPVIVVKVVRGCWILYEKANFEGHSLALEEGELELSSIWGTEEMLDEEAESDKPVVIGSIRHVVQDYRISQIDLFTEPEGLGLLNSYFDDTEEMQGFPVMQKTCSIKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEEAYIGSMRPLKMGGRKVEFPTDPKVVIYEKPSFEGRCMELETEMSSFIMEGGETEETTGNNNLPFTSVGSMKVLRGVWVAYEKCGFTGHQYLLEEGEYRDWNAWGGYNGELQSLRPILSDFSNAHMIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYVLDKGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGQSQSFEETIDQIDDSFQTKSCRVLGGSWVAYDGENFSGNQYVLEEGHYPCLSAMGCLPGATLKSLRFIDFEFSEPTIILFERENFKGKKIELNAETVNLRSLGFNTQIRSVRVVGGIWVTYEYGNYRGRQFLLSPAEVPNWYEFSACRQIGSLRPFAQKRIYFRLRNKATGLFMSTNGNLEDLKLLRVQVMEDVGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNVDSQFWCMKSDGRIYSKMKPNLVLDVKGGAQYDQNHIILNTVSTEKLTQVWEAMVL

Target ORF information:

RefSeq Version	XM_006512498.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus absent in melanoma 1 (Aim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512498.3

ORF Insert Sequence:

ATGCCGTTGT CGCCACCATC GCAGGGCGAC CCCAGGGAGG CCAGCCCGTC CAAGGCCCCC 
AAGAAACACC CCACCTTCCA TATCTGGCGC TCCAAGAAGA AGCAGTCTCC ATCGTCCAGC 
TGTGGGGTGT TTATCCCTCA CCCGCCCGGG GCTTTCGGGG AGACCAGAGC TTTCCTTGTA 
CCCGATGGAA GCCAAGGGGA GCCCCAGGAG TTAACACTGC ATGGTGGTGA ACTACAGTTA 
TCTGACACCC CCAGTGAGGT GCCTGGCCCT TCATCTACGG AGTCTGCAAT GTTAAAAAAG 
TCCAGAGCAC AACCACCAGA AGACACCAGA AGGAAGCCAG TGTTGGAAAA ACTTGGCACT 
CTTTTTACGG CTGGAAGGAG AAGGAACACC AGGAACGGAT TAGAGAGTCC CACCAACTCA 
AATAGCAAAT CGGCCTCTGC TAAAGTCCCA AAAACAGGGA GTGAGAAAAC TAAGGCTCAG 
GACAACCAAC TAAAGGCAAC CGACGCTGGC GAGGCGGGCC CCAGGCAGGA GGCTGAGGGC 
CAGCCCGTTG AGAGCTGTGT TCAGGCAACC CCCTGCGACG CCGAACAGTC ACCTTGCTCC 
AGCATCAATG TAGGGGTTGT GCAGCAGTGC CACAGCAATG ATTCAGCCCA ATTAGAACCT 
CTACAGGCAG AGGGAGAGAC TTTCCCAGAT GCCACCACCG CTGCCAAAGA GCTGCTGCAT 
TCCTCACCAG GGAATCGCTC CAGGCAGGAG AGCGCAGAGA CGCCCGCCCG CAGTCCGGGG 
GAGGAAGCGT TGCCTGGCGC TGGCCTCCAA CCAGAGACAG CGAGAGGTGT GTCGGGGTCG 
CCGCCAGAGG AGCACCTCGT GGGAGGAGTA GGAGAGGCCC CCGACGGAGC CCCCGGCGTG 
GGTGCCGAAG CGGGCTCTCT GGGGGCCCCG GGGGACGCGG TTCCTCCAGG TGAGCCTCCC 
GCCCAGGACG CAGAGACCAG CCGCACAGCC GGGTCGGTCG ACCGCGCGCA TCCCACCAAA 
GTGCTAACGT TGGACATCTA CTTGAGCAAA ACTGAGGTGG CTCAGGTGGA CGAGCCGGCC 
TCGATTGCTC CGGGGACAGA CGACTGCGAG GATTCGGACG ACATGGAAAA GAGGTCAAGC 
GGCCGCCGGT CTGGTCGGCG GAGGAAGTCG CAGAAATCCA CCGACTCCCC GGGTGCGGAT 
GCGGTGGCTC CCGAGAGTGC GGCCAAGGAC GAGGCGGTGT TCGACGACGA GGTGGCGCCA 
GACGCTGCGG CTGAGAACTG CTTGGCGGAA AGGAAAGTGA AATCGGTACA AGCGGTTCCC 
GACGGAGGCG CAGCCCCGGT CGCCTGCCCG GAGCCAAAGG CCAGCTCCTG CCCCAGAGGG 
CAACCCCGAG GGGAACCGGA CCGCAGCAAG CCCCTGTCGC CCACCAAGCG GAAGGGTCGC 
AGCCGTGTCC CCGAGGCGGT GCCCAGCTCG CCCGGCAGTG GCTCGCGCGC TCCCGCCAAG 
GAGTCCCCAC CCAAGAGGGC GCCCGCTGCG GCGGACAGCG GCGAGGAGGC GGGCCGCGTC 
GTCCCGCGCG AGCTCACCGT CAAAAGCAGC TCGTTGCTGC CCGAGATCAA GCCAGAGCAC 
AAGAGGGGTC CTCTGCCCCA CCACTTTGAT AGCCGGGGCG ACGGGGGTCG CAGCAGGGGA 
ACAGGAGCGT CCGACGCCGA CGGCCTGAAG CCCAGGAACC AGTTCGGTGT GGGCAGGTCT 
ACTGTGACCA CCAAAGTGAC CCTCCCCGCC AGACCCAAAC ATGTGGAACT AAATCTGAAA 
ACTCCGAAGA ATCCTGACAG TTTGGGAAAT GAGCACAACT CATTTAGCCA GCCAGTTCAT 
AAGGGGAACA CAGCCAACAA AATCTCCTTA TTTGAAAACA AGCGGGCTAA CAGTAGCCCC 
CGCCACCCCG ACGTCCGTAA CACAAGGAAC AATCCTCCCT CTAGTAAGAC ATTTGTTGGG 
AGGGCGAAGC TGAACTTAGC CAAAAAGGCC AAAGAAATGG AGCAACCCGA AAAGAAAGCT 
GTGTCAACCA GCCACCAGAA TGGCCTGGTG GTCAAAGAGC CATCTGCAGA AGGGAAAGGC 
ACAGCAGTCC CTGTCCCCAG AGAAGAGATT CGTCCAGCCA CCAGAGGAGG GGAAGGAGAG 
TCCACCAAGG ATCAGGTCCT CCGCCCTGAG CCTGAGCAAA GTGATAAGCT GGATGTACAA 
ACAGACACTG CCTATCCTTC CCACCCAGGG CCCACTGCTC TGGTTCCTGT GAAGGACTCT 
GAGCTCTCAG GAGAGGGCCA GCCAGAGGCT GCTGACAGCA AAGGCCCCGT CCTTAACAAC 
ACAAGTGATG TTGCACAGGA CATCGCTGCC ATTTGTGATG CCAGAGAGGC TCCCCCAAAG 
CCACAGGATG CATTTTCTGA CTCACAGCCA GCTTCAGCGG CTGAGTCAGC TAGGGAGTCA 
TCTCTCCCGC CTTCTGCACC CATTTCTGTG GACGCTCCCC AAGATGGCTG TGTTCAAGTT 
CCCATATGCA GTGTTCCCTG CACTGCTCTA GAAGTGTCAG AAAACCACAA TGGGTGTGTT 
TCGCCAGTCT CTCCTCACAC TGAGAAAACT CCCATCTCCA AACCTGGAGT GGGAGAAGCC 
AGTCCCCCTT CCACTCCAGA GCACAGCCCA GAAACTATGG GGAAAGACTG TCCCTCCAAA 
GTCCTCGTCC AGGTCCGGTC CTTTGTGCTT CCCGTGGAGA ATCCTCAGGA TGTGAGCTCT 
CAGGTCATCT CAGAGAGCTC TGAAGTTAGA GAGGCACAGC TGCCAAGCTG TCACAGTAAT 
GAGCCTGAAG TGGTGTCTGT CACCACTTGT GTTCCGCAGC ACGAGGATGC ACCAGACACA 
TCACCCAAGG ACATCCATCA CAGAGAAGGA CATGTGGCAC AGTCTCGACC ACACATCACA 
CAGTCAGGAC CTGAGAAGGC TCCGCCCATC CAGAGCAGCA GTGGGATGCC CCAGACAGCG 
GAGTTCATCC TCACCCACTC TCCCAGCAGC AGAAGTCATG CAGAAGGCCC CCAAAGGCCA 
GGTCAAGATG CGACCAGCCC ACTTACCCCT ACCAGGCTTT TGAACATTTC TGCTGGTAGT 
GACAATAGTG TCTTGGATTC TTCTTCTGAT ATGGAAAAAT TCACTGAAAT TATTAAAAAG 
ATGGACAGTG CAGTATGTGT GCCCCAGAAA AAGAAGAAGG CCCGGGTCCC AAACTCTCCA 
GCCCCTCACT TCGCTATGCC TCCCATTCAC GAGGACAGTT TGGAAAAGGT GTTTGATCCC 
AACGTGTTCA CCATTGGTTT AGGGAAGAAG AAGGAAAGCC TGGCAGAGGT GTCACCAGCT 
TTGCACTTGT TGCAGAACCA CGACCCCATA TCCAAACTGA GAGCCAAGCG TGCATCCACC 
GAGCAGAGCA TCGTCTTCAA GTCCTTGCAC ACTAACAGTA ATGGGAAGAG CGAGCCACAG 
ACCACTTCAG AAGCAAGTGA CAAGGAGAAC AGGGACATCA ACAGCGGCGG CGTTAAGAGG 
TCACGGCTAG AAAACAGCAC GCTTTTCTCA AGCATGCTTT CTTCTCTGCC ACAAGACAAA 
GTCTTTTCTC CCTCCGTGAC GTCAGTCAAC ACAATGAGCA CATCTTTTAG CACTTCTCAG 
AACAGCTCCG TATCTCAGTC ATCAGTGGTA CAGCCCAGGA AGGAGGGTGG CCTGCCCTGT 
GGCTCAGACA AAGAACAGCC ACACCTTGCA CCCCACAACT CCTTAAAGGT CTTCAATTTC 
AATTCATCAA ATACATCTCG TCTGGGTTCC AGCCACATGG AAAAGTACCC ACAAAAAGAG 
GGAAGCAAGG AAGATGTGAA TTCTCAGGGC AACCTACAAT TGCCAGAAAA CAACCTTTCA 
GACTTACCCA AACTGAAGAA CAGCGATGGC GTGGAAGGTA TTCTGAAGTC AAACCTGCCG 
AATGTTGGGA GCGTGGACGC GGACTTCCTG GGCCTTTTCA AAGCAAGCCG GTATGACCCA 
GGCCATTCCT TTTCTGGAAT GTCCTTATCA GACTCTATGA CTCTTAGAGG AAGTATGCAC 
AGCAAACTCA ACCCCCGGCC TGGCAAGGTA GTGATATTCA GTGAGCCCGA CGTCTCTGAG 
GAGTGCATTG AGGTGTTTGG GGACATCCAG GACTGCAGCT CCTGGCGCCT CTCTCCAGTG 
ATAGTAGTGA AAGTTGTCCG AGGATGTTGG ATTTTGTATG AGAAAGCCAA TTTTGAAGGG 
CATTCTCTTG CTTTAGAAGA AGGTGAACTG GAACTCTCTA GTATCTGGGG AACAGAGGAA 
ATGTTGGATG AAGAAGCAGA GTCTGATAAA CCTGTAGTGA TTGGTTCCAT CAGACACGTG 
GTTCAGGATT ACAGAATTAG TCAGATCGAC TTGTTTACTG AGCCTGAAGG GTTAGGACTC 
CTGAACTCGT ACTTTGATGA CACTGAGGAA ATGCAGGGCT TCCCTGTGAT GCAGAAGACG 
TGTTCCATCA AAGTGCACTG GGGCACATGG CTGATCTATG AAGAGCCTGG ATTCCAAGGT 
GTCCCCTTCA TTCTGGAGCC CGGAGAATAC CCTGACCTAT CCTTCTGGGA TACCGAGGAA 
GCGTACATCG GATCCATGCG GCCTCTGAAA ATGGGTGGCC GTAAAGTTGA ATTCCCTACA 
GATCCAAAGG TAGTTATTTA TGAAAAGCCT TCCTTTGAAG GAAGGTGTAT GGAGCTAGAA 
ACAGAAATGA GTAGTTTTAT CATGGAAGGA GGTGAGACAG AAGAAACAAC TGGAAACAAC 
AACTTGCCCT TTACATCCGT GGGGTCTATG AAAGTCCTAA GAGGCGTCTG GGTTGCTTAT 
GAGAAGTGTG GCTTCACTGG CCACCAGTAT TTGCTGGAGG AGGGAGAATA CAGGGACTGG 
AACGCCTGGG GAGGCTACAA TGGAGAGCTC CAGTCTCTGC GGCCTATATT AAGTGATTTT 
TCAAATGCGC ACATGATAAT GTACAGTGAG AAGAACTTTG GCTCCAAAGG TTCCAGTATT 
GATGTGTTGG GAATTGTTGC CAATTTGAAG GAGACCGGCT ACGGAGTGAA GACACAGTCA 
ATCAATGTAC TGAGTGGCGT GTGGGTAGCC TATGAAAACC CGGACTTCAC GGGGGAGCAG 
TATGTCCTGG ATAAAGGCTT CTACACCAGT TTTGAGGATT GGGGAGGCAA AAATTGTAAG 
ATCTCTTCCG TCCAACCCAT ATGCCTGGAT TCTTTCACTG GCCCCAGGAG ACGAAATCAG 
ATTCACTTGT TTTCAGAACC ACAGTTCCAA GGTCAAAGTC AAAGTTTTGA AGAAACAATA 
GATCAGATTG ACGACTCCTT TCAGACCAAG TCTTGCAGAG TTTTGGGAGG CAGCTGGGTT 
GCTTATGATG GAGAAAATTT CTCTGGCAAT CAGTATGTAC TGGAAGAAGG CCACTATCCT 
TGTCTCTCGG CAATGGGATG CCTGCCTGGA GCAACCCTGA AGTCTCTTCG CTTTATAGAT 
TTTGAATTTT CCGAACCAAC AATTATTCTT TTCGAAAGAG AAAACTTCAA AGGAAAAAAG 
ATAGAACTGA ACGCAGAAAC AGTCAATCTC CGATCCTTGG GGTTCAACAC TCAGATCCGC 
TCTGTGCGGG TCGTTGGTGG CATATGGGTT ACCTATGAAT ATGGCAATTA CAGAGGCCGG 
CAGTTCCTGC TGTCTCCTGC AGAAGTACCA AACTGGTATG AGTTCAGTGC CTGTCGCCAA 
ATAGGCTCCC TCCGGCCCTT TGCTCAGAAA CGAATTTACT TCAGACTTCG AAACAAAGCA 
ACAGGACTAT TTATGTCAAC CAACGGGAAC TTGGAAGACC TGAAGCTTCT CCGAGTCCAG 
GTCATGGAAG ATGTTGGTGC TGATGATCAG ATCTGGATCT ATCAAGAAGG GTGCATCAAA 
TGCAGGATAG CAGAAGACTG CTGCCTGACG ATCGTGGGCA GCCTCGTCAC GTCTGGCTCC 
AAACTAGGAC TGGCCCTCGA TCAAAATGTC GACAGTCAGT TCTGGTGCAT GAAGTCCGAC 
GGCAGGATCT ACAGCAAGAT GAAACCAAAT TTAGTGTTGG ATGTGAAAGG GGGTGCGCAG 
TATGATCAAA ATCACATCAT CCTCAACACA GTGAGCACAG AGAAGTTAAC ACAAGTGTGG 
GAAGCCATGG TCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Developmental biology360(2)415-25(2011 Dec)
Tamplin OJ,Cox BJ,Rossant J

Physical and transcriptional map of the mouse Chromosome 10 proximal region syntenic to human 6q16-q21.
Mammalian genome : official journal of the International Mammalian Genome Society12(12)887-92(2001 Dec)
Chalhoub N,Benachenhou N,Vacher J

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS

Cloning and tissue expression of the mouse ortholog of AIM1, a betagamma-crystallin superfamily member.
Mammalian genome : official journal of the International Mammalian Genome Society9(9)715-20(1998 Sep)
Teichmann U,Ray ME,Ellison J,Graham C,Wistow G,Meltzer PS,Trent JM,Pavan WJ

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Aim1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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