Nr0b1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nr0b1
Entrez Gene ID	11614
Full Name	nuclear receptor subfamily 0, group B, member 1
Synonyms	AHX,Ahc,Ahch,DAX-1,Dax1
General protein information	Preferred Names nuclear receptor subfamily 0, group B, member 1 Names nuclear receptor subfamily 0 group B member 1 adrenal hypoplasia congenita-like protein adrenal hypoplasia, congenital homolog nuclear receptor DAX-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X C1\|X 39.67 cM
Summary	This gene encodes an orphan nuclear receptor protein that plays a key role in differentiation of the gonads. This protein regulates steroidogenic factor 1 (Sf-1) in a dose-dependent manner, sometimes functioning as a repressor of SF-1 target genes, and sometimes functioning as a co-activator. Overexpression of this gene can cause feminization of the XY male gonads. This gene is also involved in the maintenance of embryonic stem cell pluripotancy. Mutations in the related gene in human cause congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, May 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007430.5	NP_031456.1	nuclear receptor subfamily 0 group B member 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NR0B1	NP_000466.2
Canis lupus familiaris (dog)	NR0B1	XP_003640302.1
Gallus gallus (chicken)	NR0B1	NP_989924.1
Bos taurus (cattle)	NR0B1	NP_001192786.1
Mus musculus (house mouse)	Nr0b1	NP_031456.1
Rattus norvegicus (Norway rat)	Nr0b1	NP_445769.1
Pan troglodytes (chimpanzee)	NR0B1	NP_001181864.1
	NR0B1	XP_002806222.1
Danio rerio (zebrafish)	nr0b1	NP_001076416.1
Xenopus tropicalis (tropical clawed frog)	LOC100487043	XP_002933661.1

Gene Ontology
Bibliography

Related articles in PubMed

Timing of adrenal regression controlled by synergistic interaction between Sf1 SUMOylation and Dax1.
Xing Y, Morohashi KI, Ingraham HA, Hammer GD
Development (Cambridge, England)144(20)3798-3807(2017 10)

Ex vivo cultures combined with vivo-morpholino induced gene knockdown provide a system to assess the role of WT1 and GATA4 during gonad differentiation.
Rudigier LJ, Dame C, Scholz H, Kirschner KM
PloS one12(4)e0176296(2017)

Esrrb directly binds to Gata6 promoter and regulates its expression with Dax1 and Ncoa3.
Uranishi K, Akagi T, Koide H, Yokota T
Biochemical and biophysical research communications478(4)1720-5(2016 09)

Identification of NR0B1 as a novel androgen receptor co-repressor in mouse Sertoli cells.
Li YC, Luo ML, Guo H, Wang TT, Lin SR, Chen JB, Ma Q, Gu YL, Jiang ZM, Gui YT
International journal of molecular medicine38(3)853-60(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sf1 SUMOylation and Dax1 have roles in the physiological cessation of FAdE-mediated Sf1 expression and the resultant regression of the postnatal fetal cortex (X-zone)
Title: Timing of adrenal regression controlled by synergistic interaction between Sf1 SUMOylation and Dax1.

Findings indicate that the Gata6 promoter is activated by Esrrb in association with Ncoa3, and Dax1 inhibited activities of Esrrb and Ncoa3, resulting maintenance of the undifferentiated status of embryonic stem (ES) cells.
Title: Esrrb directly binds to Gata6 promoter and regulates its expression with Dax1 and Ncoa3.

Data indicate that nuclear receptor subfamily 0 group B member 1 (Nr0b1) interacted with androgen receptor (AR) in Sertoli cells (SCs).
Title: Identification of NR0B1 as a novel androgen receptor co-repressor in mouse Sertoli cells.

CpG methylation within the NR0B1 promoter is not involved in the in vivo regulation of NR0B1 expression, whereas the hyperacetylation of histone H4 and the unmethylation of histones H3K9 and H3K27, and their binding to the NR0B1 promoter results in decondensed euchromatin for NR0B1 activation.
Title: Epigenetic modifications promote the expression of the orphan nuclear receptor NR0B1 in human lung adenocarcinoma cells.

Here we review the current knowledge on properties, functions and mechanisms of DAX1 action.
Title: [DAX1-unusual member of nuclear receptors superfamily with diverse functions].

The following Nr0b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nr0b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13612	NM_007430.5 Latest version!	Mus musculus nuclear receptor subfamily 0, group B, member 1 (Nr0b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$279.30 $399.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nr0b1 ( NM_007430.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031456.1 Mus musculus nuclear receptor subfamily 0, group B, member 1 (Nr0b1), mRNA.

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CloneID	OMu13612
Clone ID Related Accession (Same CDS sequence)	NM_007430.5
Accession Version	NM_007430.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	nuclear receptor subfamily 0 group B member 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK141239.1, AK133411.1 and BB404032.1. On May 5, 2015 this sequence version replaced NM_007430.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U41568.1, AK141239.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTG AGGACCACCC GTGGCAGGGC AGCATCCTCT ACAATCTACT GATGAGCGCG 
AAGCAGAAGC ACGCGTCTCA GGAAGAGCGA GAGGTGCGCT TGGGGGCTCA GTGCTGGGGT 
TGCGCCTGCG GTGCTCAGCC CGTCCTGGGT GGGGAGAGAC TGTCCGGCGG GCAAGCCAGG 
TCCCTCTTGT ACCGCTGCTG CTTTTGTGGG GAGAATCACC CGCGCCAGGG TGGCATCCTC 
TACTCTATGC TCACCAACGC CAGGCAGCCA AGCGTGGCGA CCCAGGCGCC GAGGGCACGA 
TTCGGAGCAC CTTGCTGGGG CTGCGCCTGC GGCAGCGCAG AGCCCCTGGT GGGCAGAGAG 
GGGCTGCCGG CTGGCCAGGC CCCCTCGCTC CTGTACCGCT GCTGCTTCTG CGGAGAAGAG 
CACCCGAGGC AGGGCAGCAT CTTATACAGC TTGCTCACTA GCGCTCAGCA AACGCACGTG 
TCTCGGGAAG CACCCGAGGC ACATCGCAGA GGCGAGTGGT GGCAGCTGTC CTACTGTACC 
CAGAGTGTGG GTGGCCCAGA GGGGCTGCAG AGCACACAGG CCATGGCGTT CCTGTACCGC 
AGCTATGTGT GCGGTGAAGA GCAGCCCCAG CAGATCAGCG TTGCCTCTGG CACGCCCGTG 
AGCGCAGACC AAACACCAGC GACCCCGCAA GAGCAGCCGA GGGCTCCCTG GTGGGACGCC 
TCACCTGGTG TGCAGCGTCT GATCACACTC AAGGATCCAC AGGTGGTGTG CGAGGCAGCG 
TCCGCTGGCC TGTTGAAGAC CCTGCGCTTT GTCAAGTACT TGCCCTGCTT CCAGATCCTG 
CCCCTAGATC AGCAGCTGGT GCTGGTGCGG AGCTGTTGGG CGCCCCTACT CATGCTTGAG 
TTGGCCCAAG ATCACCTGCA CTTCGAGATG ATGGAGATCC CGGAGACCAA CACGACGCAG 
GAAATGCTTA CCACCAGGCG GCAGGAGACC GAAGGTCCAG AGCCTGCAGA GCCCCAGGCC 
ACAGAGCAGC CACAGATGGT GTCCGCGGAG GCTGGGCACT TGCTCCCAGC TGCTGCGGTC 
CAGGCCATCA AGAGTTTCTT TTTCAAGTGC TGGAGTCTGA ACATTGACAC CAAAGAGTAT 
GCCTATCTGA AAGGGACCGT GCTCTTTAAC CCAGACCTGC CTGGCCTGCA GTGCGTGAAA 
TACATTGAGG GTCTTCAGTG GAGAACCCAG CAGATCCTTA CTGAGCACAT CCGGATGATG 
CAGAGAGAGT ACCAGATCAG ATCCGCTGAA CTGAACAGTG CCCTTTTCCT GCTGAGATTC 
ATCAATAGCG ATGTCGTCAC TGAACTCTTT TTCAGGCCCA TCATTGGTGC AGTCAGCATG 
GATGATATGA TGCTGGAGAT GCTCTGTGCA AAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031456.1
CDS	27..1445
Translation	MAGEDHPWQGSILYNLLMSAKQKHASQEEREVRLGAQCWGCACGAQPVLGGERLSGGQARSLLYRCCFCGENHPRQGGILYSMLTNARQPSVATQAPRARFGAPCWGCACGSAEPLVGREGLPAGQAPSLLYRCCFCGEEHPRQGSILYSLLTSAQQTHVSREAPEAHRRGEWWQLSYCTQSVGGPEGLQSTQAMAFLYRSYVCGEEQPQQISVASGTPVSADQTPATPQEQPRAPWWDASPGVQRLITLKDPQVVCEAASAGLLKTLRFVKYLPCFQILPLDQQLVLVRSCWAPLLMLELAQDHLHFEMMEIPETNTTQEMLTTRRQETEGPEPAEPQATEQPQMVSAEAGHLLPAAAVQAIKSFFFKCWSLNIDTKEYAYLKGTVLFNPDLPGLQCVKYIEGLQWRTQQILTEHIRMMQREYQIRSAELNSALFLLRFINSDVVTELFFRPIIGAVSMDDMMLEMLCAKL

Target ORF information:

RefSeq Version	NM_007430.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus nuclear receptor subfamily 0, group B, member 1 (Nr0b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007430.5

ORF Insert Sequence:

ATGGCGGGTG AGGACCACCC GTGGCAGGGC AGCATCCTCT ACAATCTACT GATGAGCGCG 
AAGCAGAAGC ACGCGTCTCA GGAAGAGCGA GAGGTGCGCT TGGGGGCTCA GTGCTGGGGT 
TGCGCCTGCG GTGCTCAGCC CGTCCTGGGT GGGGAGAGAC TGTCCGGCGG GCAAGCCAGG 
TCCCTCTTGT ACCGCTGCTG CTTTTGTGGG GAGAATCACC CGCGCCAGGG TGGCATCCTC 
TACTCTATGC TCACCAACGC CAGGCAGCCA AGCGTGGCGA CCCAGGCGCC GAGGGCACGA 
TTCGGAGCAC CTTGCTGGGG CTGCGCCTGC GGCAGCGCAG AGCCCCTGGT GGGCAGAGAG 
GGGCTGCCGG CTGGCCAGGC CCCCTCGCTC CTGTACCGCT GCTGCTTCTG CGGAGAAGAG 
CACCCGAGGC AGGGCAGCAT CTTATACAGC TTGCTCACTA GCGCTCAGCA AACGCACGTG 
TCTCGGGAAG CACCCGAGGC ACATCGCAGA GGCGAGTGGT GGCAGCTGTC CTACTGTACC 
CAGAGTGTGG GTGGCCCAGA GGGGCTGCAG AGCACACAGG CCATGGCGTT CCTGTACCGC 
AGCTATGTGT GCGGTGAAGA GCAGCCCCAG CAGATCAGCG TTGCCTCTGG CACGCCCGTG 
AGCGCAGACC AAACACCAGC GACCCCGCAA GAGCAGCCGA GGGCTCCCTG GTGGGACGCC 
TCACCTGGTG TGCAGCGTCT GATCACACTC AAGGATCCAC AGGTGGTGTG CGAGGCAGCG 
TCCGCTGGCC TGTTGAAGAC CCTGCGCTTT GTCAAGTACT TGCCCTGCTT CCAGATCCTG 
CCCCTAGATC AGCAGCTGGT GCTGGTGCGG AGCTGTTGGG CGCCCCTACT CATGCTTGAG 
TTGGCCCAAG ATCACCTGCA CTTCGAGATG ATGGAGATCC CGGAGACCAA CACGACGCAG 
GAAATGCTTA CCACCAGGCG GCAGGAGACC GAAGGTCCAG AGCCTGCAGA GCCCCAGGCC 
ACAGAGCAGC CACAGATGGT GTCCGCGGAG GCTGGGCACT TGCTCCCAGC TGCTGCGGTC 
CAGGCCATCA AGAGTTTCTT TTTCAAGTGC TGGAGTCTGA ACATTGACAC CAAAGAGTAT 
GCCTATCTGA AAGGGACCGT GCTCTTTAAC CCAGACCTGC CTGGCCTGCA GTGCGTGAAA 
TACATTGAGG GTCTTCAGTG GAGAACCCAG CAGATCCTTA CTGAGCACAT CCGGATGATG 
CAGAGAGAGT ACCAGATCAG ATCCGCTGAA CTGAACAGTG CCCTTTTCCT GCTGAGATTC 
ATCAATAGCG ATGTCGTCAC TGAACTCTTT TTCAGGCCCA TCATTGGTGC AGTCAGCATG 
GATGATATGA TGCTGGAGAT GCTCTGTGCA AAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Timing of adrenal regression controlled by synergistic interaction between Sf1 SUMOylation and Dax1.
Development (Cambridge, England)144(20)3798-3807(2017 10)
Xing Y,Morohashi KI,Ingraham HA,Hammer GD

Ex vivo cultures combined with vivo-morpholino induced gene knockdown provide a system to assess the role of WT1 and GATA4 during gonad differentiation.
PloS one12(4)e0176296(2017)
Rudigier LJ,Dame C,Scholz H,Kirschner KM

Esrrb directly binds to Gata6 promoter and regulates its expression with Dax1 and Ncoa3.
Biochemical and biophysical research communications478(4)1720-5(2016 09)
Uranishi K,Akagi T,Koide H,Yokota T

Identification of NR0B1 as a novel androgen receptor co-repressor in mouse Sertoli cells.
International journal of molecular medicine38(3)853-60(2016 Sep)
Li YC,Luo ML,Guo H,Wang TT,Lin SR,Chen JB,Ma Q,Gu YL,Jiang ZM,Gui YT

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Nr0b1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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