Adam12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adam12
Entrez Gene ID	11489
Full Name	a disintegrin and metallopeptidase domain 12 (meltrin alpha)
Synonyms	Mltna
General protein information	Preferred Names a disintegrin and metallopeptidase domain 12 (meltrin alpha) Names disintegrin and metalloproteinase domain-containing protein 12 a disintegrin and metalloprotease domain 12 a disintegrin and metalloproteinase domain 12 (meltrin alpha)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F3
Summary	This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein that localizes to the cell surface. About a third of the mice lacking the encoded protein die before weaning. Overexpression of the encoded protein in a mouse model of Duchenne muscular dystrophy alleviates the muscle pathology by preventing cell necrosis and inflammation. [provided by RefSeq, May 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007400.2	NP_031426.2	disintegrin and metalloproteinase domain-containing protein 12 preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Adam12	XP_006230508.1
Bos taurus (cattle)	ADAM12	NP_001001156.1
Caenorhabditis elegans (roundworm)	adm-2	NP_510291.1
Homo sapiens (human)	ADAM12	NP_003465.3
Canis lupus familiaris (dog)	ADAM12	XP_005637899.1
Gallus gallus (chicken)	ADAM12	NP_001136322.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013468	XP_003436497.1
Danio rerio (zebrafish)	adam12	XP_691731.6
Xenopus tropicalis (tropical clawed frog)	adam12	NP_001035103.1
Pan troglodytes (chimpanzee)	ADAM12	XP_508106.2
	ADAM12	XP_001087980.2
Mus musculus (house mouse)	Adam12	NP_031426.2

Gene Ontology
Bibliography

Related articles in PubMed

Modulation of miR29a improves impaired post-ischemic angiogenesis in hyperglycemia.
Chen L, Okeke E, Ayalew D, Wang D, Shahid L, Dokun AO
Experimental biology and medicine (Maywood, N.J.)242(14)1432-1443(2017 08)

Tumor necrosis factor-? alters integrins and metalloprotease ADAM12 levels and signaling in differentiating myoblasts.
Grzelkowska-Kowalczyk K, Tokarska J, Grabiec K, Gajewska M, Milewska M, B?aszczyk M
Polish journal of veterinary sciences19(2)253-9(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In conclusion, MiR29a regulates endothelial cell ADAM12 upregulation in ischemia and this is impaired in hyperglycemia.
Title: Modulation of miR29a improves impaired post-ischemic angiogenesis in hyperglycemia.

It was concluded that TNF-alpha-induced changes in extracellular matix components increased expression of ADAM12 among other changes that were temporally related with the onset of myocyte function.
Title: Tumor necrosis factor-? alters integrins and metalloprotease ADAM12 levels and signaling in differentiating myoblasts.

Augmentation of ADAM12 expression in vivo improved outcomes in Balb/c mice, whereas knockdown of ADAM12 made outcomes worse in C57Bl/6 mice. In vitro, ADAM12 expression modulates endothelial cell proliferation, survival, and angiogenesis.
Title: ADAM12: a genetic modifier of preclinical peripheral arterial disease.

ADAM12 and ADAM17 are essential molecules for the impairment of barrier function of retinal vasculature under hypoxia.
Title: ADAM12 and ADAM17 are essential molecules for hypoxia-induced impairment of neural vascular barrier function.

The effect of insulin-like growth factor-I on ADAM12 expression during the course of myogenesis is reported.
Title: Insulin-Like Growth Factor-I Increases Laminin, Integrin Subunits and Metalloprotease ADAM12 in Mouse Myoblasts.

The following Adam12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adam12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13281	NM_007400.2 Latest version!	Mus musculus a disintegrin and metallopeptidase domain 12 (meltrin alpha) (Adam12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adam12 ( NM_007400.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031426.2 Mus musculus a disintegrin and metallopeptidase domain 12 (meltrin alpha) (Adam12), mRNA.

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CloneID	OMu13281
Clone ID Related Accession (Same CDS sequence)	NM_007400.2
Accession Version	NM_007400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	disintegrin and metalloproteinase domain-containing protein 12 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC126676.3, AC140055.4 and AC125372.4. On Nov 8, 2006 this sequence version replaced NM_007400.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D50411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC GCCCGGCGCG GCGCGCGCCC CCCGCCCGCG CCCTCCTGCT GGCCCTGGCT 
GGGGCCCTGC TGGCGCCCCG TGCAGCCCGA GGGATGAGTT TGTGGGACCA GAGAGGAACT 
TACGAAGTGG CCAGAGCCTC CCTTCTGAGC AAGGACCCTG GGATCCCAGG ACAGAGCATC 
CCAGCCAAGG ATCATCCAGA CGTGCTGACT GTGCAACTGC AGCTGGAGAG CCGAGACCTG 
ATCCTCAGCC TGGAAAGGAA TGAGGGACTC ATTGCCAATG GCTTCACGGA GACCCATTAT 
CTGCAAGATG GTACTGATGT CTCTCTCACT CGAAATCACA CGGATCATTG TTACTACCAT 
GGACATGTGC AAGGAGATGC TGCATCAGTG GTCAGCCTCA GTACTTGCTC TGGTCTCCGG 
GGACTTATCA TGTTTGAAAA TAAAACGTAC AGCTTAGAGC CAATGAAAAA CACCACTGAC 
AGCTACAAAC TCGTCCCAGC TGAGAGCATG ACGAACATCC AAGGGCTGTG TGGGTCACAG 
CATAACAAGT CCAACCTCAC CATGGAAGAT GTCTCCCCTG GAACCTCTCA AATGCGGGCA 
AGAAGGCATA AGAGAGAGAC CCTTAAGATG ACCAAGTACG TAGAGCTGGT TATTGTGGCA 
GACAACAGAG AGTTTCAGAG GCAAGGAAAA GACCTGGAGA AAGTTAAGCA GCGATTAATA 
GAGATCGCCA ATCACGTTGA CAAGTTTTAC AGACCACTGA ACATCCGGAT CGTGCTGGTA 
GGAGTGGAAG TGTGGAATGA CATCGACAAA TGCTCTATAA GCCAGGACCC ATTCACCAGC 
CTCCATGAGT TTCTAGACTG GAGAAAGATA AAGCTTCTAC CTCGAAAATC CCACGACAAT 
GCTCAGCTTA TCAGTGGGGT TTATTTCCAA GGAACCACCA TCGGCATGGC ACCCATCATG 
AGCATGTGCA CTGCAGAACA GTCTGGAGGA GTTGTCATGG ACCATTCAGA CAGCCCCCTT 
GGTGCCGCAG TGACCTTGGC ACATGAGCTG GGCCACAACT TCGGGATGAA CCATGACACA 
CTGGAGAGGG GCTGCAGCTG CAGAATGGCC GCAGAGAAAG GAGGCTGCAT CATGAACCCG 
TCCACGGGGT TCCCATTCCC CATGGTGTTC AGCAGCTGCA GCAGGAAGGA CCTGGAGGCT 
AGCCTGGAGA AGGGCATGGG GATGTGCCTC TTCAACCTAC CAGAGGTCAA GCAGGCCTTT 
GGGGGCCGGA AGTGTGGAAA TGGCTATGTG GAAGAGGGAG AAGAGTGTGA CTGCGGAGAA 
CCGGAGGAAT GCACGAATCG CTGCTGTAAC GCTACCACCT GTACTCTGAA GCCAGATGCT 
GTGTGCGCGC ACGGGCAGTG CTGTGAAGAC TGTCAGCTGA AGCCTCCAGG AACTGCATGC 
AGGGGCTCCA GCAACTCCTG TGACCTCCCA GAATTCTGCA CAGGGACTGC CCCTCACTGT 
CCAGCCAATG TGTACCTACA TGATGGCCAC CCGTGTCAGG GCGTGGATGG TTACTGCTAC 
AACGGCATCT GCCAGACCCA TGAGCAGCAG TGTGTCACGC TCTGGGGACC AGGTGCTAAA 
CCGGCTCCTG GCATCTGCTT TGAGCGAGTC AACTCTGCAG GAGATCCTTA TGGTAACTGT 
GGCAAAGACT CCAAGAGCGC CTTCGCCAAA TGTGAGCTGA GAGATGCCAA GTGTGGGAAA 
ATCCAGTGTC AAGGTGGTGC AAGCCGACCT GTCATTGGTA CCAATGCTGT TTCCATAGAA 
ACAAATATCC CACAGCAGGA AGGAGGTCGG ATTCTGTGCC GGGGGACCCA TGTGTACTTG 
GGTGATGACA TGCCAGACCC AGGGCTTGTG CTTGCAGGAA CAAAGTGTGC AGAAGGAAAA 
ATCTGCCTCA ATCGTCGATG TCAGAATATC AGTGTCTTCG GCGTTCACAA GTGTGCCATG 
CAGTGCCACG GCCGAGGGGT ATGTAACAAC AGGAAGAATT GCCACTGTGA AGCCCACTGG 
GCTCCACCCT TCTGTGACAA GTTTGGCTTT GGAGGAAGCA CAGACAGTGG TCCCATCAGG 
CAAGCAGATA ACCAGGGCTT GACTGTAGGA ATCCTGGTGA GCATCCTGTG TCTGCTTGCT 
GCTGGATTTG TGGTGTATCT CAAAAGGAAG ACGTTGATGC GGCTGCTGTT CACACATAAA 
AAAACCACCA TGGAAAAGCT AAGGTGTGTG CACCCTTCCC GGACACCCAG TGGCCCTCAC 
CTTGGCCAGG CTCACCACAC CCCCGGGAAA GGCCTGCTGA TGAACCGGGC ACCACATTTC 
AATACCCCCA AGGACAGGCA CTCGCTGAAA TGCCAGAACA TGGACATCAG CAGGCCCCTC 
GACGCTCGAG CCGTCCCACA GCTTCAGTCA CCTCAGCGAG TGCTCCTGCC TCTCCACCAG 
ACCCCACGTG CACCCAGTGG CCCTGCCAGG CCCCTGCCCG CCAGTCCTGC AGTCAGGCAG 
GCCCAGGGCA TTCGAAAACC CAGTCCTCCT CAGAAGCCTC TGCCTGCTGA TCCACTGAGC 
AGGACTTCTC GGCTCACTAG TGCCTTGGTG AGGACCCCAG GGCAGCAGGA ACCTGGGCAC 
CGCCCAGCCC CCATCAGACC TGCCCCTAAG CATCAAGTAC CCAGACCTTC CCACAATGCC 
TATATCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031426.2
CDS	221..2932
Translation	MAERPARRAPPARALLLALAGALLAPRAARGMSLWDQRGTYEVARASLLSKDPGIPGQSIPAKDHPDVLTVQLQLESRDLILSLERNEGLIANGFTETHYLQDGTDVSLTRNHTDHCYYHGHVQGDAASVVSLSTCSGLRGLIMFENKTYSLEPMKNTTDSYKLVPAESMTNIQGLCGSQHNKSNLTMEDVSPGTSQMRARRHKRETLKMTKYVELVIVADNREFQRQGKDLEKVKQRLIEIANHVDKFYRPLNIRIVLVGVEVWNDIDKCSISQDPFTSLHEFLDWRKIKLLPRKSHDNAQLISGVYFQGTTIGMAPIMSMCTAEQSGGVVMDHSDSPLGAAVTLAHELGHNFGMNHDTLERGCSCRMAAEKGGCIMNPSTGFPFPMVFSSCSRKDLEASLEKGMGMCLFNLPEVKQAFGGRKCGNGYVEEGEECDCGEPEECTNRCCNATTCTLKPDAVCAHGQCCEDCQLKPPGTACRGSSNSCDLPEFCTGTAPHCPANVYLHDGHPCQGVDGYCYNGICQTHEQQCVTLWGPGAKPAPGICFERVNSAGDPYGNCGKDSKSAFAKCELRDAKCGKIQCQGGASRPVIGTNAVSIETNIPQQEGGRILCRGTHVYLGDDMPDPGLVLAGTKCAEGKICLNRRCQNISVFGVHKCAMQCHGRGVCNNRKNCHCEAHWAPPFCDKFGFGGSTDSGPIRQADNQGLTVGILVSILCLLAAGFVVYLKRKTLMRLLFTHKKTTMEKLRCVHPSRTPSGPHLGQAHHTPGKGLLMNRAPHFNTPKDRHSLKCQNMDISRPLDARAVPQLQSPQRVLLPLHQTPRAPSGPARPLPASPAVRQAQGIRKPSPPQKPLPADPLSRTSRLTSALVRTPGQQEPGHRPAPIRPAPKHQVPRPSHNAYIK

Target ORF information:

RefSeq Version	NM_007400.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus a disintegrin and metallopeptidase domain 12 (meltrin alpha) (Adam12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007400.2

ORF Insert Sequence:

ATGGCAGAGC GCCCGGCGCG GCGCGCGCCC CCCGCCCGCG CCCTCCTGCT GGCCCTGGCT 
GGGGCCCTGC TGGCGCCCCG TGCAGCCCGA GGGATGAGTT TGTGGGACCA GAGAGGAACT 
TACGAAGTGG CCAGAGCCTC CCTTCTGAGC AAGGACCCTG GGATCCCAGG ACAGAGCATC 
CCAGCCAAGG ATCATCCAGA CGTGCTGACT GTGCAACTGC AGCTGGAGAG CCGAGACCTG 
ATCCTCAGCC TGGAAAGGAA TGAGGGACTC ATTGCCAATG GCTTCACGGA GACCCATTAT 
CTGCAAGATG GTACTGATGT CTCTCTCACT CGAAATCACA CGGATCATTG TTACTACCAT 
GGACATGTGC AAGGAGATGC TGCATCAGTG GTCAGCCTCA GTACTTGCTC TGGTCTCCGG 
GGACTTATCA TGTTTGAAAA TAAAACGTAC AGCTTAGAGC CAATGAAAAA CACCACTGAC 
AGCTACAAAC TCGTCCCAGC TGAGAGCATG ACGAACATCC AAGGGCTGTG TGGGTCACAG 
CATAACAAGT CCAACCTCAC CATGGAAGAT GTCTCCCCTG GAACCTCTCA AATGCGGGCA 
AGAAGGCATA AGAGAGAGAC CCTTAAGATG ACCAAGTACG TAGAGCTGGT TATTGTGGCA 
GACAACAGAG AGTTTCAGAG GCAAGGAAAA GACCTGGAGA AAGTTAAGCA GCGATTAATA 
GAGATCGCCA ATCACGTTGA CAAGTTTTAC AGACCACTGA ACATCCGGAT CGTGCTGGTA 
GGAGTGGAAG TGTGGAATGA CATCGACAAA TGCTCTATAA GCCAGGACCC ATTCACCAGC 
CTCCATGAGT TTCTAGACTG GAGAAAGATA AAGCTTCTAC CTCGAAAATC CCACGACAAT 
GCTCAGCTTA TCAGTGGGGT TTATTTCCAA GGAACCACCA TCGGCATGGC ACCCATCATG 
AGCATGTGCA CTGCAGAACA GTCTGGAGGA GTTGTCATGG ACCATTCAGA CAGCCCCCTT 
GGTGCCGCAG TGACCTTGGC ACATGAGCTG GGCCACAACT TCGGGATGAA CCATGACACA 
CTGGAGAGGG GCTGCAGCTG CAGAATGGCC GCAGAGAAAG GAGGCTGCAT CATGAACCCG 
TCCACGGGGT TCCCATTCCC CATGGTGTTC AGCAGCTGCA GCAGGAAGGA CCTGGAGGCT 
AGCCTGGAGA AGGGCATGGG GATGTGCCTC TTCAACCTAC CAGAGGTCAA GCAGGCCTTT 
GGGGGCCGGA AGTGTGGAAA TGGCTATGTG GAAGAGGGAG AAGAGTGTGA CTGCGGAGAA 
CCGGAGGAAT GCACGAATCG CTGCTGTAAC GCTACCACCT GTACTCTGAA GCCAGATGCT 
GTGTGCGCGC ACGGGCAGTG CTGTGAAGAC TGTCAGCTGA AGCCTCCAGG AACTGCATGC 
AGGGGCTCCA GCAACTCCTG TGACCTCCCA GAATTCTGCA CAGGGACTGC CCCTCACTGT 
CCAGCCAATG TGTACCTACA TGATGGCCAC CCGTGTCAGG GCGTGGATGG TTACTGCTAC 
AACGGCATCT GCCAGACCCA TGAGCAGCAG TGTGTCACGC TCTGGGGACC AGGTGCTAAA 
CCGGCTCCTG GCATCTGCTT TGAGCGAGTC AACTCTGCAG GAGATCCTTA TGGTAACTGT 
GGCAAAGACT CCAAGAGCGC CTTCGCCAAA TGTGAGCTGA GAGATGCCAA GTGTGGGAAA 
ATCCAGTGTC AAGGTGGTGC AAGCCGACCT GTCATTGGTA CCAATGCTGT TTCCATAGAA 
ACAAATATCC CACAGCAGGA AGGAGGTCGG ATTCTGTGCC GGGGGACCCA TGTGTACTTG 
GGTGATGACA TGCCAGACCC AGGGCTTGTG CTTGCAGGAA CAAAGTGTGC AGAAGGAAAA 
ATCTGCCTCA ATCGTCGATG TCAGAATATC AGTGTCTTCG GCGTTCACAA GTGTGCCATG 
CAGTGCCACG GCCGAGGGGT ATGTAACAAC AGGAAGAATT GCCACTGTGA AGCCCACTGG 
GCTCCACCCT TCTGTGACAA GTTTGGCTTT GGAGGAAGCA CAGACAGTGG TCCCATCAGG 
CAAGCAGATA ACCAGGGCTT GACTGTAGGA ATCCTGGTGA GCATCCTGTG TCTGCTTGCT 
GCTGGATTTG TGGTGTATCT CAAAAGGAAG ACGTTGATGC GGCTGCTGTT CACACATAAA 
AAAACCACCA TGGAAAAGCT AAGGTGTGTG CACCCTTCCC GGACACCCAG TGGCCCTCAC 
CTTGGCCAGG CTCACCACAC CCCCGGGAAA GGCCTGCTGA TGAACCGGGC ACCACATTTC 
AATACCCCCA AGGACAGGCA CTCGCTGAAA TGCCAGAACA TGGACATCAG CAGGCCCCTC 
GACGCTCGAG CCGTCCCACA GCTTCAGTCA CCTCAGCGAG TGCTCCTGCC TCTCCACCAG 
ACCCCACGTG CACCCAGTGG CCCTGCCAGG CCCCTGCCCG CCAGTCCTGC AGTCAGGCAG 
GCCCAGGGCA TTCGAAAACC CAGTCCTCCT CAGAAGCCTC TGCCTGCTGA TCCACTGAGC 
AGGACTTCTC GGCTCACTAG TGCCTTGGTG AGGACCCCAG GGCAGCAGGA ACCTGGGCAC 
CGCCCAGCCC CCATCAGACC TGCCCCTAAG CATCAAGTAC CCAGACCTTC CCACAATGCC 
TATATCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modulation of miR29a improves impaired post-ischemic angiogenesis in hyperglycemia.
Experimental biology and medicine (Maywood, N.J.)242(14)1432-1443(2017 08)
Chen L,Okeke E,Ayalew D,Wang D,Shahid L,Dokun AO

Tumor necrosis factor-? alters integrins and metalloprotease ADAM12 levels and signaling in differentiating myoblasts.
Polish journal of veterinary sciences19(2)253-9(2016)
Grzelkowska-Kowalczyk K,Tokarska J,Grabiec K,Gajewska M,Milewska M,B?aszczyk M

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Adam12 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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