Ada cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ada
Entrez Gene ID	11486
Full Name	adenosine deaminase
General protein information	Preferred Names adenosine deaminase Names adenosine deaminase adenosine aminohydrolase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 84.44 cM\|2 H3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272052.1	NP_001258981.1	adenosine deaminase
NM_007398.4	NP_031424.1	adenosine deaminase

Pathways from BioSystems

Homologies

	ADA	XP_001109908.2
Rattus norvegicus (Norway rat)	Ada	NP_569083.1
Kluyveromyces lactis	KLLA0E23167g	XP_454997.1
Homo sapiens (human)	ADA	NP_000013.2
Danio rerio (zebrafish)	ada	NP_001002646.1
Saccharomyces cerevisiae (baker's yeast)	AAH1	NP_014258.1
Canis lupus familiaris (dog)	ADA	XP_005635156.1
Gallus gallus (chicken)	ADA	NP_001006290.1
Schizosaccharomyces pombe (fission yeast)	dea2	NP_595071.1
Xenopus tropicalis (tropical clawed frog)	ada	NP_001011025.1
Mus musculus (house mouse)	Ada	NP_001258981.1
Pan troglodytes (chimpanzee)	ADA	XP_001144517.1
Bos taurus (cattle)	ADA	NP_776312.1
Caenorhabditis elegans (roundworm)	C06G3.5	NP_501087.1

Gene Ontology
Bibliography

Related articles in PubMed

Sustained Elevated Adenosine via ADORA2B Promotes Chronic Pain through Neuro-immune Interaction.
Hu X, Adebiyi MG, Luo J, Sun K, Le TT, Zhang Y, Wu H, Zhao S, Karmouty-Quintana H, Liu H, Huang A, Wen YE, Zaika OL, Mamenko M, Pochynyuk OM, Kellems RE, Eltzschig HK, Blackburn MR, Walters ET, Huang D, Hu H, Xia Y
Cell reports16(1)106-119(2016 06)

Elevated adenosine signaling via adenosine A2B receptor induces normal and sickle erythrocyte sphingosine kinase 1 activity.
Sun K, Zhang Y, Bogdanov MV, Wu H, Song A, Li J, Dowhan W, Idowu M, Juneja HS, Molina JG, Blackburn MR, Kellems RE, Xia Y
Blood125(10)1643-52(2015 Mar)

Elevated placental adenosine signaling contributes to the pathogenesis of preeclampsia.
Iriyama T, Sun K, Parchim NF, Li J, Zhao C, Song A, Hart LA, Blackwell SC, Sibai BM, Chan LN, Chan TS, Hicks MJ, Blackburn MR, Kellems RE, Xia Y
Circulation131(8)730-41(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pulmonary alveolar proteinosis in adenosine deaminase-deficient mice
Title: Pulmonary alveolar proteinosis in adenosine deaminase-deficient mice.

Data indicate that untreated murine adenosine deaminase ADA(-/-) Tregs show alterations in the plasma membrane CD39/CD73. ectonucleotidase machinery and limited suppressive activity via extracellular adenosine.
Title: Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID.

ADA is upregulated in NOD dendritic cells, which results in their exuberant and spontaneous activation, which triggers autoimmune T cell activation.
Title: High levels of adenosine deaminase on dendritic cells promote autoreactive T cell activation and diabetes in nonobese diabetic mice.

Adenosine deaminase is necessary but not sufficient to confer puromycin aminonucleoside sensitivity to podocytes.
Title: Over-expression of adenosine deaminase in mouse podocytes does not reverse puromycin aminonucleoside resistance.

Dietary regulation of adenosine deaminase activity in stomach, small intestine and spleen of mice
Title: Dietary regulation of adenosine deaminase activity in stomach, small intestine and spleen of mice.

The following Ada gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ada cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00103	NM_007398.4 Latest version!	Mus musculus adenosine deaminase (Ada), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu00103	NM_001272052.1 Latest version!	Mus musculus adenosine deaminase (Ada), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ada ( NM_001272052.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258981.1 Mus musculus adenosine deaminase (Ada), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00103
Clone ID Related Accession (Same CDS sequence)	NM_001272052.1 , NM_007398.4
Accession Version	NM_001272052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	adenosine deaminase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591490.12, AK075899.1 and D19191.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK075899.1, AF483480.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGA CACCCGCATT CAACAAACCC AAAGTAGAGT TACACGTCCA CCTGGATGGA 
GCCATCAAGC CAGAAACCAT CTTATACTTT GGCAAGAAGA GAGGCATCGC CCTCCCGGCA 
GATACAGTGG AGGAGCTGCG CAACATTATC GGCATGGACA AGCCCCTCTC GCTCCCAGGC 
TTCCTGGCCA AGTTTGACTA CTACATGCCT GTGATTGCGG GCTGCAGAGA GGCCATCAAG 
AGGATCGCCT ACGAGTTTGT GGAGATGAAG GCAAAGGAGG GCGTGGTCTA TGTGGAAGTG 
CGCTATAGCC CACACCTGCT GGCCAATTCC AAGGTGGACC CAATGCCCTG GAACCAGACT 
GAAGGGGACG TCACCCCTGA TGACGTTGTG GATCTTGTGA ACCAGGGCCT GCAGGAGGGA 
GAGCAAGCAT TTGGCATCAA GGTCCGGTCC ATTCTGTGCT GCATGCGCCA CCAGCCCAGC 
TGGTCCCTTG AGGTGTTGGA GCTGTGTAAG AAGTACAATC AGAAGACCGT GGTGGCTATG 
GACTTGGCTG GGGATGAGAC CATTGAAGGA AGTAGCCTCT TCCCAGGCCA CGTGGAAGCC 
TATGAGGGCG CAGTAAAGAA TGGCATTCAT CGGACCGTCC ACGCTGGCGA GGTGGGCTCT 
CCTGAGGTTG TGCGTGAGGC TGTGGACATC CTCAAGACAG AGAGGGTGGG ACATGGTTAT 
CACACCATCG AGGATGAAGC TCTCTACAAC AGACTACTGA AAGAAAACAT GCACTTTGAG 
GTCTGCCCCT GGTCCAGCTA CCTCACAGGC GCCTGGGATC CCAAAACGAC GCATGCGGTT 
GTTCGCTTCA AGAATGATAA GGCCAACTAC TCACTCAACA CAGACGACCC CCTCATCTTC 
AAGTCCACCC TAGACACTGA CTACCAGATG ACCAAGAAAG ACATGGGCTT CACTGAGGAG 
GAGTTCAAGC GACTGAACAT CAACGCAGCG AAGTCAAGCT TCCTCCCAGA GGAAGAGAAG 
AAGGAACTTC TGGAACGGCT CTACAGAGAA TACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258981.1
CDS	149..1207
Translation	MAQTPAFNKPKVELHVHLDGAIKPETILYFGKKRGIALPADTVEELRNIIGMDKPLSLPGFLAKFDYYMPVIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVDPMPWNQTEGDVTPDDVVDLVNQGLQEGEQAFGIKVRSILCCMRHQPSWSLEVLELCKKYNQKTVVAMDLAGDETIEGSSLFPGHVEAYEGAVKNGIHRTVHAGEVGSPEVVREAVDILKTERVGHGYHTIEDEALYNRLLKENMHFEVCPWSSYLTGAWDPKTTHAVVRFKNDKANYSLNTDDPLIFKSTLDTDYQMTKKDMGFTEEEFKRLNINAAKSSFLPEEEKKELLERLYREYQ

Target ORF information:

RefSeq Version	NM_001272052.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenosine deaminase (Ada), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272052.1

ORF Insert Sequence:

ATGGCCCAGA CACCCGCATT CAACAAACCC AAAGTAGAGT TACACGTCCA CCTGGATGGA 
GCCATCAAGC CAGAAACCAT CTTATACTTT GGCAAGAAGA GAGGCATCGC CCTCCCGGCA 
GATACAGTGG AGGAGCTGCG CAACATTATC GGCATGGACA AGCCCCTCTC GCTCCCAGGC 
TTCCTGGCCA AGTTTGACTA CTACATGCCT GTGATTGCGG GCTGCAGAGA GGCCATCAAG 
AGGATCGCCT ACGAGTTTGT GGAGATGAAG GCAAAGGAGG GCGTGGTCTA TGTGGAAGTG 
CGCTATAGCC CACACCTGCT GGCCAATTCC AAGGTGGACC CAATGCCCTG GAACCAGACT 
GAAGGGGACG TCACCCCTGA TGACGTTGTG GATCTTGTGA ACCAGGGCCT GCAGGAGGGA 
GAGCAAGCAT TTGGCATCAA GGTCCGGTCC ATTCTGTGCT GCATGCGCCA CCAGCCCAGC 
TGGTCCCTTG AGGTGTTGGA GCTGTGTAAG AAGTACAATC AGAAGACCGT GGTGGCTATG 
GACTTGGCTG GGGATGAGAC CATTGAAGGA AGTAGCCTCT TCCCAGGCCA CGTGGAAGCC 
TATGAGGGCG CAGTAAAGAA TGGCATTCAT CGGACCGTCC ACGCTGGCGA GGTGGGCTCT 
CCTGAGGTTG TGCGTGAGGC TGTGGACATC CTCAAGACAG AGAGGGTGGG ACATGGTTAT 
CACACCATCG AGGATGAAGC TCTCTACAAC AGACTACTGA AAGAAAACAT GCACTTTGAG 
GTCTGCCCCT GGTCCAGCTA CCTCACAGGC GCCTGGGATC CCAAAACGAC GCATGCGGTT 
GTTCGCTTCA AGAATGATAA GGCCAACTAC TCACTCAACA CAGACGACCC CCTCATCTTC 
AAGTCCACCC TAGACACTGA CTACCAGATG ACCAAGAAAG ACATGGGCTT CACTGAGGAG 
GAGTTCAAGC GACTGAACAT CAACGCAGCG AAGTCAAGCT TCCTCCCAGA GGAAGAGAAG 
AAGGAACTTC TGGAACGGCT CTACAGAGAA TACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ada ( NM_007398.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031424.1 Mus musculus adenosine deaminase (Ada), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00103
Clone ID Related Accession (Same CDS sequence)	NM_001272052.1 , NM_007398.4
Accession Version	NM_007398.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	adenosine deaminase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591490.12 and AK075899.1. On Jan 4, 2013 this sequence version replaced NM_007398.3. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK075899.1, BC002075.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGA CACCCGCATT CAACAAACCC AAAGTAGAGT TACACGTCCA CCTGGATGGA 
GCCATCAAGC CAGAAACCAT CTTATACTTT GGCAAGAAGA GAGGCATCGC CCTCCCGGCA 
GATACAGTGG AGGAGCTGCG CAACATTATC GGCATGGACA AGCCCCTCTC GCTCCCAGGC 
TTCCTGGCCA AGTTTGACTA CTACATGCCT GTGATTGCGG GCTGCAGAGA GGCCATCAAG 
AGGATCGCCT ACGAGTTTGT GGAGATGAAG GCAAAGGAGG GCGTGGTCTA TGTGGAAGTG 
CGCTATAGCC CACACCTGCT GGCCAATTCC AAGGTGGACC CAATGCCCTG GAACCAGACT 
GAAGGGGACG TCACCCCTGA TGACGTTGTG GATCTTGTGA ACCAGGGCCT GCAGGAGGGA 
GAGCAAGCAT TTGGCATCAA GGTCCGGTCC ATTCTGTGCT GCATGCGCCA CCAGCCCAGC 
TGGTCCCTTG AGGTGTTGGA GCTGTGTAAG AAGTACAATC AGAAGACCGT GGTGGCTATG 
GACTTGGCTG GGGATGAGAC CATTGAAGGA AGTAGCCTCT TCCCAGGCCA CGTGGAAGCC 
TATGAGGGCG CAGTAAAGAA TGGCATTCAT CGGACCGTCC ACGCTGGCGA GGTGGGCTCT 
CCTGAGGTTG TGCGTGAGGC TGTGGACATC CTCAAGACAG AGAGGGTGGG ACATGGTTAT 
CACACCATCG AGGATGAAGC TCTCTACAAC AGACTACTGA AAGAAAACAT GCACTTTGAG 
GTCTGCCCCT GGTCCAGCTA CCTCACAGGC GCCTGGGATC CCAAAACGAC GCATGCGGTT 
GTTCGCTTCA AGAATGATAA GGCCAACTAC TCACTCAACA CAGACGACCC CCTCATCTTC 
AAGTCCACCC TAGACACTGA CTACCAGATG ACCAAGAAAG ACATGGGCTT CACTGAGGAG 
GAGTTCAAGC GACTGAACAT CAACGCAGCG AAGTCAAGCT TCCTCCCAGA GGAAGAGAAG 
AAGGAACTTC TGGAACGGCT CTACAGAGAA TACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031424.1
CDS	149..1207
Translation	MAQTPAFNKPKVELHVHLDGAIKPETILYFGKKRGIALPADTVEELRNIIGMDKPLSLPGFLAKFDYYMPVIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVDPMPWNQTEGDVTPDDVVDLVNQGLQEGEQAFGIKVRSILCCMRHQPSWSLEVLELCKKYNQKTVVAMDLAGDETIEGSSLFPGHVEAYEGAVKNGIHRTVHAGEVGSPEVVREAVDILKTERVGHGYHTIEDEALYNRLLKENMHFEVCPWSSYLTGAWDPKTTHAVVRFKNDKANYSLNTDDPLIFKSTLDTDYQMTKKDMGFTEEEFKRLNINAAKSSFLPEEEKKELLERLYREYQ

Target ORF information:

RefSeq Version	NM_007398.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenosine deaminase (Ada), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007398.4

ORF Insert Sequence:

ATGGCCCAGA CACCCGCATT CAACAAACCC AAAGTAGAGT TACACGTCCA CCTGGATGGA 
GCCATCAAGC CAGAAACCAT CTTATACTTT GGCAAGAAGA GAGGCATCGC CCTCCCGGCA 
GATACAGTGG AGGAGCTGCG CAACATTATC GGCATGGACA AGCCCCTCTC GCTCCCAGGC 
TTCCTGGCCA AGTTTGACTA CTACATGCCT GTGATTGCGG GCTGCAGAGA GGCCATCAAG 
AGGATCGCCT ACGAGTTTGT GGAGATGAAG GCAAAGGAGG GCGTGGTCTA TGTGGAAGTG 
CGCTATAGCC CACACCTGCT GGCCAATTCC AAGGTGGACC CAATGCCCTG GAACCAGACT 
GAAGGGGACG TCACCCCTGA TGACGTTGTG GATCTTGTGA ACCAGGGCCT GCAGGAGGGA 
GAGCAAGCAT TTGGCATCAA GGTCCGGTCC ATTCTGTGCT GCATGCGCCA CCAGCCCAGC 
TGGTCCCTTG AGGTGTTGGA GCTGTGTAAG AAGTACAATC AGAAGACCGT GGTGGCTATG 
GACTTGGCTG GGGATGAGAC CATTGAAGGA AGTAGCCTCT TCCCAGGCCA CGTGGAAGCC 
TATGAGGGCG CAGTAAAGAA TGGCATTCAT CGGACCGTCC ACGCTGGCGA GGTGGGCTCT 
CCTGAGGTTG TGCGTGAGGC TGTGGACATC CTCAAGACAG AGAGGGTGGG ACATGGTTAT 
CACACCATCG AGGATGAAGC TCTCTACAAC AGACTACTGA AAGAAAACAT GCACTTTGAG 
GTCTGCCCCT GGTCCAGCTA CCTCACAGGC GCCTGGGATC CCAAAACGAC GCATGCGGTT 
GTTCGCTTCA AGAATGATAA GGCCAACTAC TCACTCAACA CAGACGACCC CCTCATCTTC 
AAGTCCACCC TAGACACTGA CTACCAGATG ACCAAGAAAG ACATGGGCTT CACTGAGGAG 
GAGTTCAAGC GACTGAACAT CAACGCAGCG AAGTCAAGCT TCCTCCCAGA GGAAGAGAAG 
AAGGAACTTC TGGAACGGCT CTACAGAGAA TACCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sustained Elevated Adenosine via ADORA2B Promotes Chronic Pain through Neuro-immune Interaction.
Cell reports16(1)106-119(2016 06)
Hu X,Adebiyi MG,Luo J,Sun K,Le TT,Zhang Y,Wu H,Zhao S,Karmouty-Quintana H,Liu H,Huang A,Wen YE,Zaika OL,Mamenko M,Pochynyuk OM,Kellems RE,Eltzschig HK,Blackburn MR,Walters ET,Huang D,Hu H,Xia Y

Elevated adenosine signaling via adenosine A2B receptor induces normal and sickle erythrocyte sphingosine kinase 1 activity.
Blood125(10)1643-52(2015 Mar)
Sun K,Zhang Y,Bogdanov MV,Wu H,Song A,Li J,Dowhan W,Idowu M,Juneja HS,Molina JG,Blackburn MR,Kellems RE,Xia Y

Elevated placental adenosine signaling contributes to the pathogenesis of preeclampsia.
Circulation131(8)730-41(2015 Feb)
Iriyama T,Sun K,Parchim NF,Li J,Zhao C,Song A,Hart LA,Blackwell SC,Sibai BM,Chan LN,Chan TS,Hicks MJ,Blackburn MR,Kellems RE,Xia Y

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Ada cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報