Spred1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spred1
Entrez Gene ID	114715
Full Name	sprouty protein with EVH-1 domain 1, related sequence
Synonyms	5730461F13Rik,AL024345,AW047647
General protein information	Preferred Names sprouty protein with EVH-1 domain 1, related sequence Names sprouty-related, EVH1 domain-containing protein 1 sprouty-related protein 1 with EVH-1 domain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5
Summary	This gene encodes a membrane-associated protein that is phosphorylated by tyrosine kinases in response to growth factors. The encoded protein acts as a negative regulator of the mitogen-activated protein (MAP) kinase signaling pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_033524.3	NP_277059.1	sprouty-related, EVH1 domain-containing protein 1 isoform 1
NM_001277256.1	NP_001264185.1	sprouty-related, EVH1 domain-containing protein 1 isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPRED1	XP_544609.3
Rattus norvegicus (Norway rat)	Spred1	NP_001040554.1
Xenopus tropicalis (tropical clawed frog)	spred1	NP_001008037.1
Gallus gallus (chicken)	SPRED1	NP_001186638.1
Pan troglodytes (chimpanzee)	SPRED1	XP_001138171.1
Bos taurus (cattle)	SPRED1	NP_001179445.1
Mus musculus (house mouse)	Spred1	NP_277059.1
Danio rerio (zebrafish)	spred1	NP_998397.1
Homo sapiens (human)	SPRED1	NP_689807.1

Gene Ontology
Bibliography

Related articles in PubMed

TPPU enhanced exercise-induced epoxyeicosatrienoic acid concentrations to exert cardioprotection in mice after myocardial infarction.
Guo Y, Luo F, Zhang X, Chen J, Shen L, Zhu Y, Xu D
Journal of cellular and molecular medicine(2017 Dec)

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Rutledge EA, Benazet JD, McMahon AP
Development (Cambridge, England)144(17)3177-3188(2017 09)

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
Heng X, Guo Q, Leung AW, Li JY
eLife6(2017 May)

Sprouty proteins: modified modulators, matchmakers or missing links?
Guy GR, Jackson RA, Yusoff P, Chow SY
The Journal of endocrinology203(2)191-202(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data suggest that Spred1 negatively regulates group 2 innate lymphoid cell development and functions through the suppression of the Ras-ERK pathway.
Title: Spred1, a Suppressor of the Ras-ERK Pathway, Negatively Regulates Expansion and Function of Group 2 Innate Lymphoid Cells.

SPREDs promote self-renewal and inhibit mesodermal differentiation of murine ES cells by selective suppression of the ERK/MAPK signaling pathway in pluripotent cells
Title: SPREDs (Sprouty related proteins with EVH1 domain) promote self-renewal and inhibit mesodermal differentiation in murine embryonic stem cells.

Cosuppression of Sprouty and Sprouty-related negative regulators of FGF signalling in prostate cancer
Title: Cosuppression of Sprouty and Sprouty-related negative regulators of FGF signalling in prostate cancer: a working hypothesis.

Microrna-126 was transported into recipient human coronary artery endothelial cells by endothelial microparticles and functionally regulated the target protein sprouty-related, EVH1 domain-containing protein 1 (SPRED1).
Title: Endothelial microparticle-mediated transfer of MicroRNA-126 promotes vascular endothelial cell repair via SPRED1 and is abrogated in glucose-damaged endothelial microparticles.

show that neurofibromin, the NF1 gene product, is a Spred1-interacting protein that is necessary for Spred1's inhibitory function
Title: A shared molecular mechanism underlies the human rasopathies Legius syndrome and Neurofibromatosis-1.

The following Spred1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spred1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17294	NM_001277256.1 Latest version!	Mus musculus sprouty protein with EVH-1 domain 1, related sequence (Spred1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu08939	NM_033524.3 Latest version!	Mus musculus sprouty protein with EVH-1 domain 1, related sequence (Spred1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spred1 ( NM_001277256.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001264185.1 Mus musculus sprouty protein with EVH-1 domain 1, related sequence (Spred1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17294
Clone ID Related Accession (Same CDS sequence)	NM_001277256.1
Accession Version	NM_001277256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Mus musculus(house mouse)
Product	sprouty-related, EVH1 domain-containing protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL807741.8, BC079606.1 and AL928959.9. Transcript Variant: This variant (2) lacks two 3' coding exons and its transcription extends past a splice site that is used in variant 1, resulting in a distinct 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC057874.1, BI647295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCA GAGATGACTC AAGTGGTGGA TGGCTACCAC TCGGAGGGAG CGGACTGAGC 
AGCGTCACTG TCTTCAGAGT CCCTCATCAG GAAGAGAATG GCTGTGCGGA CTTTTTTATC 
CGTGGAGAGA GACTCAGAGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA GAAAGACCTC 
ATCTATAATA AGGTCACTCC CACATTTCAC CACTGGAAGA TCGATGACAA GAAGTTTGGC 
CTTACCTTTC AGAGTCCTGC TGATGCCAGG GCTTTTGATC GAGGCATTCG AAGAGCTATA 
GAGGATATAT CTCTAGGGTG CCCAGCGTCA AAAACTGAGG CTGAAGGAGG AGATGATGAT 
TTACAAACGA CTGAAGAAGA CACTTCCCGT TCCCTAGTGA AAGATCACTT TTTCCAGCAA 
GAGACAGTTG TTACCAGTGA ACCTTACAGA AGCTCAGACA TAAGACCGTT ACCCTTTGAA 
GATCTGAATG CCAGAAGAGT CTACTTGCAA AGCCAAGTCA GCCAGGTGAG AAGACGAATA 
CTGTTTCCAT GCATAGCTTA TATAGAAAAG CTGGGTGTGC TACAGAACAC CAAGGCCCTC 
CGCTCTTTTC CATGTTACGA AACGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264185.1
CDS	655..1344
Translation	MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFRVPHQEENGCADFFIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISLGCPASKTEAEGGDDDLQTTEEDTSRSLVKDHFFQQETVVTSEPYRSSDIRPLPFEDLNARRVYLQSQVSQVRRRILFPCIAYIEKLGVLQNTKALRSFPCYETI

Target ORF information:

RefSeq Version	NM_001277256.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sprouty protein with EVH-1 domain 1, related sequence (Spred1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277256.1

ORF Insert Sequence:

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCA GAGATGACTC AAGTGGTGGA TGGCTACCAC TCGGAGGGAG CGGACTGAGC 
AGCGTCACTG TCTTCAGAGT CCCTCATCAG GAAGAGAATG GCTGTGCGGA CTTTTTTATC 
CGTGGAGAGA GACTCAGAGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA GAAAGACCTC 
ATCTATAATA AGGTCACTCC CACATTTCAC CACTGGAAGA TCGATGACAA GAAGTTTGGC 
CTTACCTTTC AGAGTCCTGC TGATGCCAGG GCTTTTGATC GAGGCATTCG AAGAGCTATA 
GAGGATATAT CTCTAGGGTG CCCAGCGTCA AAAACTGAGG CTGAAGGAGG AGATGATGAT 
TTACAAACGA CTGAAGAAGA CACTTCCCGT TCCCTAGTGA AAGATCACTT TTTCCAGCAA 
GAGACAGTTG TTACCAGTGA ACCTTACAGA AGCTCAGACA TAAGACCGTT ACCCTTTGAA 
GATCTGAATG CCAGAAGAGT CTACTTGCAA AGCCAAGTCA GCCAGGTGAG AAGACGAATA 
CTGTTTCCAT GCATAGCTTA TATAGAAAAG CTGGGTGTGC TACAGAACAC CAAGGCCCTC 
CGCTCTTTTC CATGTTACGA AACGATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spred1 ( NM_033524.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_277059.1 Mus musculus sprouty protein with EVH-1 domain 1, related sequence (Spred1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08939
Clone ID Related Accession (Same CDS sequence)	NM_033524.3
Accession Version	NM_033524.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Mus musculus(house mouse)
Product	sprouty-related, EVH1 domain-containing protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL807741.8, BC079606.1 and AL928959.9. On Mar 26, 2013 this sequence version replaced NM_033524.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.59782.1, AK155065.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCA GAGATGACTC AAGTGGTGGA TGGCTACCAC TCGGAGGGAG CGGACTGAGC 
AGCGTCACTG TCTTCAGAGT CCCTCATCAG GAAGAGAATG GCTGTGCGGA CTTTTTTATC 
CGTGGAGAGA GACTCAGAGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA GAAAGACCTC 
ATCTATAATA AGGTCACTCC CACATTTCAC CACTGGAAGA TCGATGACAA GAAGTTTGGC 
CTTACCTTTC AGAGTCCTGC TGATGCCAGG GCTTTTGATC GAGGCATTCG AAGAGCTATA 
GAGGATATAT CTCTAGGGTG CCCAGCGTCA AAAACTGAGG CTGAAGGAGG AGATGATGAT 
TTACAAACGA CTGAAGAAGA CACTTCCCGT TCCCTAGTGA AAGATCACTT TTTCCAGCAA 
GAGACAGTTG TTACCAGTGA ACCTTACAGA AGCTCAGACA TAAGACCGTT ACCCTTTGAA 
GATCTGAATG CCAGAAGAGT CTACTTGCAA AGCCAAGTCA GCCAGATACC ATTCAGTCAG 
CAAGGCCTAG ACATTCAGAG CCGAAGTATG GAATATGTAC AGCGGCAAAT ATCTAAGGAA 
TGTGGGAGCC TAAAGTCCCA AACTAGGGTC CCTTTGAAGT CAATCAGACA TGTCAGCTTT 
CAAGATGAGG ATGAGATTGT TAGAATAAAT CCTCGAGATA TCTTAATACG TCGATATGCA 
GACTACAGAC ATCCGGACAT GTGGAAAAAT GACTTGGAGA GAGATGATAC TGACTCCAGT 
GTTCCATTTT CTAAACAAGA CAGTAAAAAG TCAGACTATC TCTACCACTG TGGGGATGAG 
ACTAAGTTAA GTTCTCTGAA AGACTCTGTG GTATTTAAGA CACAGCCTCC CTCGTTAAAA 
TTTAAGTCAA AACGAAGAAA AGAGGATGGG GAACGTTCTC GCTGCGTATA CTGCCAGGAA 
AGGTTTAATC ATGAAGAAAA CGCCAGGGGG AAATGCCAGG ACGCTCCAGA TCCTGTTAAA 
AGATGCATAT ATCAAGTTAG CTGCATGCTC TGTGCTGAGA GCATGCTATA TCATTGTATG 
TCAGACTCAG AGGGAGACTT TTCTGATCCT TGTTCGTGTG ACACTAGCGA TGACAAGTTC 
TGCCTAAGGT GGTTAGCCCT GGTAGCTCTG TCTTTCATTG TACCATGTAT GTGCTGCTAC 
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGCG GGTGCTGTGG TGGGAAACAT 
AAGGCTGCTG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_277059.1
CDS	655..1989
Translation	MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFRVPHQEENGCADFFIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISLGCPASKTEAEGGDDDLQTTEEDTSRSLVKDHFFQQETVVTSEPYRSSDIRPLPFEDLNARRVYLQSQVSQIPFSQQGLDIQSRSMEYVQRQISKECGSLKSQTRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDTDSSVPFSKQDSKKSDYLYHCGDETKLSSLKDSVVFKTQPPSLKFKSKRRKEDGERSRCVYCQERFNHEENARGKCQDAPDPVKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG

Target ORF information:

RefSeq Version	NM_033524.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sprouty protein with EVH-1 domain 1, related sequence (Spred1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033524.3

ORF Insert Sequence:

ATGAGCGAGG AGACGGCGAC TTCTGACAAC GATAATAGTT ATGCACGAGT GCGAGCTGTG 
GTGATGACCA GAGATGACTC AAGTGGTGGA TGGCTACCAC TCGGAGGGAG CGGACTGAGC 
AGCGTCACTG TCTTCAGAGT CCCTCATCAG GAAGAGAATG GCTGTGCGGA CTTTTTTATC 
CGTGGAGAGA GACTCAGAGA CAAAATGGTG GTTTTGGAAT GTATGCTTAA GAAAGACCTC 
ATCTATAATA AGGTCACTCC CACATTTCAC CACTGGAAGA TCGATGACAA GAAGTTTGGC 
CTTACCTTTC AGAGTCCTGC TGATGCCAGG GCTTTTGATC GAGGCATTCG AAGAGCTATA 
GAGGATATAT CTCTAGGGTG CCCAGCGTCA AAAACTGAGG CTGAAGGAGG AGATGATGAT 
TTACAAACGA CTGAAGAAGA CACTTCCCGT TCCCTAGTGA AAGATCACTT TTTCCAGCAA 
GAGACAGTTG TTACCAGTGA ACCTTACAGA AGCTCAGACA TAAGACCGTT ACCCTTTGAA 
GATCTGAATG CCAGAAGAGT CTACTTGCAA AGCCAAGTCA GCCAGATACC ATTCAGTCAG 
CAAGGCCTAG ACATTCAGAG CCGAAGTATG GAATATGTAC AGCGGCAAAT ATCTAAGGAA 
TGTGGGAGCC TAAAGTCCCA AACTAGGGTC CCTTTGAAGT CAATCAGACA TGTCAGCTTT 
CAAGATGAGG ATGAGATTGT TAGAATAAAT CCTCGAGATA TCTTAATACG TCGATATGCA 
GACTACAGAC ATCCGGACAT GTGGAAAAAT GACTTGGAGA GAGATGATAC TGACTCCAGT 
GTTCCATTTT CTAAACAAGA CAGTAAAAAG TCAGACTATC TCTACCACTG TGGGGATGAG 
ACTAAGTTAA GTTCTCTGAA AGACTCTGTG GTATTTAAGA CACAGCCTCC CTCGTTAAAA 
TTTAAGTCAA AACGAAGAAA AGAGGATGGG GAACGTTCTC GCTGCGTATA CTGCCAGGAA 
AGGTTTAATC ATGAAGAAAA CGCCAGGGGG AAATGCCAGG ACGCTCCAGA TCCTGTTAAA 
AGATGCATAT ATCAAGTTAG CTGCATGCTC TGTGCTGAGA GCATGCTATA TCATTGTATG 
TCAGACTCAG AGGGAGACTT TTCTGATCCT TGTTCGTGTG ACACTAGCGA TGACAAGTTC 
TGCCTAAGGT GGTTAGCCCT GGTAGCTCTG TCTTTCATTG TACCATGTAT GTGCTGCTAC 
GTCCCTTTGA GAATGTGCCA TCGCTGTGGT GAGGCATGCG GGTGCTGTGG TGGGAAACAT 
AAGGCTGCTG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TPPU enhanced exercise-induced epoxyeicosatrienoic acid concentrations to exert cardioprotection in mice after myocardial infarction.
Journal of cellular and molecular medicine(2017 Dec)
Guo Y,Luo F,Zhang X,Chen J,Shen L,Zhu Y,Xu D

Cellular heterogeneity in the ureteric progenitor niche and distinct profiles of branching morphogenesis in organ development.
Development (Cambridge, England)144(17)3177-3188(2017 09)
Rutledge EA,Benazet JD,McMahon AP

Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia.
eLife6(2017 May)
Heng X,Guo Q,Leung AW,Li JY

Sprouty proteins: modified modulators, matchmakers or missing links?
The Journal of endocrinology203(2)191-202(2009 Nov)
Guy GR,Jackson RA,Yusoff P,Chow SY

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Spred1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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