Chrng cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrng
Entrez Gene ID	11449
Full Name	cholinergic receptor, nicotinic, gamma polypeptide
Synonyms	Achr-3,Acrg
General protein information	Preferred Names cholinergic receptor, nicotinic, gamma polypeptide Names acetylcholine receptor subunit gamma chrng variant N1 chrng variant N2 nicotinic acetylcholine receptor gamma subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C5\|1 44.07 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009604.3	NP_033734.3	acetylcholine receptor subunit gamma precursor
XM_006529078.3	XP_006529141.1	acetylcholine receptor subunit gamma isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHRNG	NP_005190.4
Pan troglodytes (chimpanzee)	CHRNG	XP_526063.1
Rattus norvegicus (Norway rat)	Chrng	NP_062018.1
Bos taurus (cattle)	CHRNG	NP_776698.1
Xenopus tropicalis (tropical clawed frog)	chrng	NP_001096503.1
Gallus gallus (chicken)	CHRNG	NP_001026739.1
Danio rerio (zebrafish)	LOC100536659	NP_001241879.1
	CHRNG	XP_001114133.2
Canis lupus familiaris (dog)	CHRNG	XP_543289.2
Mus musculus (house mouse)	Chrng	NP_033734.3

Gene Ontology
Bibliography

Related articles in PubMed

Assessment of the expression and role of the ?1-nAChR subunit in efferent cholinergic function during the development of the mammalian cochlea.
Roux I, Wu JS, McIntosh JM, Glowatzki E
Journal of neurophysiology116(2)479-92(2016 Aug)

Severe muscle wasting and denervation in mice lacking the RNA-binding protein ZFP106.
Anderson DM, Cannavino J, Li H, Anderson KM, Nelson BR, McAnally J, Bezprozvannaya S, Liu Y, Lin W, Liu N, Bassel-Duby R, Olson EN
Proceedings of the National Academy of Sciences of the United States of America113(31)E4494-503(2016 08)

Neuromuscular junction immaturity and muscle atrophy are hallmarks of the ColQ-deficient mouse, a model of congenital myasthenic syndrome with acetylcholinesterase deficiency.
Sigoillot SM, Bourgeois F, Karmouch J, Molg? J, Dobbertin A, Chevalier C, Houlgatte R, L?ger J, Legay C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(6)2382-99(2016 06)

Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle.
Sakakibara I, Wurmser M, Dos Santos M, Santolini M, Ducommun S, Davaze R, Guernec A, Sakamoto K, Maire P
Skeletal muscle6(1)30(2016)

Motor and Sensory Deficits in the teetering Mice Result from Mutation of the ESCRT Component HGS.
Watson JA, Bhattacharyya BJ, Vaden JH, Wilson JA, Icyuz M, Howard AD, Phillips E, DeSilva TM, Siegal GP, Bean AJ, King GD, Phillips SE, Miller RJ, Wilson SM
PLoS genetics11(6)e1005290(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The presence of two new transcript variants (T1 and T2) of chrng expressed in mouse postnatal day 3 and adult skeletal muscles, is reported.
Title: Alternatively spliced variants of gamma-subunit of muscle-type acetylcholine receptor in fetal and adult skeletal muscle of mouse.

Results demonstrate that the gamma-subunit is required for the formation of pre-patterned AChR clusters, which in turn play an essential role in determining the subsequent pattern of neuromuscular synaptogenesis.
Title: Essential roles of the acetylcholine receptor gamma-subunit in neuromuscular synaptic patterning.

hair cells transiently express alpha1gamma-containing nAChRs in addition to alpha9alpha10, and that these may have a role during development of the inner ear innervation
Title: The ?1 subunit of nicotinic acetylcholine receptors in the inner ear: transcriptional regulation by ATOH1 and co-expression with the ? subunit in hair cells.

Results suggest a requirement for gamma-acetylcholine receptors in mediating synaptically-induced action potential activity critical for neuromuscular development.
Title: Spontaneous muscle action potentials fail to develop without fetal-type acetylcholine receptors.

The following Chrng gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrng cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20783	NM_009604.3 Latest version!	Mus musculus cholinergic receptor, nicotinic, gamma polypeptide (Chrng), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OMu27865	XM_006529078.3 Latest version!	Mus musculus cholinergic receptor, nicotinic, gamma polypeptide (Chrng), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrng ( NM_009604.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033734.3 Mus musculus cholinergic receptor, nicotinic, gamma polypeptide (Chrng), mRNA.

Price & Availability↑

CloneID	OMu20783
Clone ID Related Accession (Same CDS sequence)	NM_009604.3
Accession Version	NM_009604.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	acetylcholine receptor subunit gamma precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK047974.1. On Dec 27, 2006 this sequence version replaced NM_009604.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK047974.1, M30514.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGCAAGGGG GCCAGAGACC TCAGCTCCTC TTGCTGCTGT TGGCTGTCTG CCTGGGGGCC 
CAGAGCCGCA ACCAAGAGGA GCGTCTGCTT GCGGACCTGA TGCGAAACTA CGACCCCCAC 
CTGCGGCCGG CTGAGCGCGA CTCAGATGTG GTCAATGTCA GCCTGAAGCT TACCTTGACC 
AACCTCATCT CCCTGAATGA ACGAGAGGAG GCCCTCACAA CTAACGTCTG GATAGAGATG 
CAATGGTGCG ACTATCGCCT GCGCTGGGAC CCAAAAGACT ACGAAGGCCT GTGGATATTG 
AGGGTGCCAT CTACTATGGT CTGGCGGCCA GATATCGTCC TGGAGAACAA TGTGGACGGT 
GTCTTCGAGG TGGCTCTCTA CTGCAATGTC CTCGTGTCCC CCGACGGTTG TATCTACTGG 
CTGCCGCCTG CCATCTTCCG CTCCTCCTGC TCCATCTCTG TCACCTACTT CCCCTTCGAT 
TGGCAGAACT GTTCCCTCGT CTTCCAATCC CAGACTTACA GCACCAGTGA GATCAACTTG 
CAGCTGAGCC AGGAGGATGG GCAAGCCATT GAGTGGATCT TCATTGACCC GGAGGCTTTC 
ACAGAGAATG GGGAGTGGGC CATCCGGCAC CGACCGGCTA AAATGCTCCT GGACTCCGTG 
GCTCCTGCAG AGGAGGCGGG CCACCAGAAG GTGGTGTTCT ACCTGCTTAT CCAGCGCAAG 
CCCCTCTTCT ACGTCATCAA CATCATCGCC CCCTGTGTGC TCATCTCCTC AGTCGCCATC 
CTCATCTACT TCCTTCCTGC TAAGGCGGGC GGGCAGAAAT GCACAGTGGC CACCAACGTG 
CTCCTGGCCC AGACTGTCTT CCTTTTCCTT GTGGCTAAGA AGGTGCCTGA GACCTCCCAG 
GCAGTGCCAC TCATCAGCAA GTACCTGACC TTCCTCATGG TGGTGACCAT CCTCATCGTC 
GTGAACTCTG TGGTCGTGCT CAATGTGTCC TTGCGGTCCC CCCACACACA CTCCATGGCC 
CGTGGGGTCC GCAAGGTGTT CCTGAGGCTC CTGCCCCAGC TGTTACGGAT GCATGTGCGC 
CCACTAGCTC CAGCTGCTGT CCAGGATGCC CGGTTCCGAC TCCAGAATGG CTCTTCCTCA 
GGGTGGCCCA TCATGGCTCG AGAGGAAGGG GACCTCTGTC TGCCTCGAAG CGAACTCCTC 
TTTAGGCAAA GGCAGCGCAA TGGATTAGTG CAGGCAGTAT TGGAGAAGCT AGAGAATGGT 
CCAGAAGTGA GGCAGAGCCA GGAGTTCTGT GGCAGCCTGA AGCAAGCCTC CCCAGCCATC 
CAGGCCTGTG TGGATGCCTG TAACCTCATG GCTCGTGCCC GACGCCAGCA GAGTCACTTT 
GACAGTGGGA ACGAGGAGTG GTTGCTGGTG GGCCGAGTGC TGGACCGAGT CTGCTTCCTA 
GCCATGCTCT CCCTCTTCAT CTGTGGCACT GCTGGCATCT TCCTCATGGC CCACTACAAC 
CAAGTGCCTG ACCTGCCGTT CCCCGGAGAC CCCCGCCCCT ACCTGCCTTT GCCAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033734.3
CDS	15..1574
Translation	MQGGQRPQLLLLLLAVCLGAQSRNQEERLLADLMRNYDPHLRPAERDSDVVNVSLKLTLTNLISLNEREEALTTNVWIEMQWCDYRLRWDPKDYEGLWILRVPSTMVWRPDIVLENNVDGVFEVALYCNVLVSPDGCIYWLPPAIFRSSCSISVTYFPFDWQNCSLVFQSQTYSTSEINLQLSQEDGQAIEWIFIDPEAFTENGEWAIRHRPAKMLLDSVAPAEEAGHQKVVFYLLIQRKPLFYVINIIAPCVLISSVAILIYFLPAKAGGQKCTVATNVLLAQTVFLFLVAKKVPETSQAVPLISKYLTFLMVVTILIVVNSVVVLNVSLRSPHTHSMARGVRKVFLRLLPQLLRMHVRPLAPAAVQDARFRLQNGSSSGWPIMAREEGDLCLPRSELLFRQRQRNGLVQAVLEKLENGPEVRQSQEFCGSLKQASPAIQACVDACNLMARARRQQSHFDSGNEEWLLVGRVLDRVCFLAMLSLFICGTAGIFLMAHYNQVPDLPFPGDPRPYLPLPD

Target ORF information:

RefSeq Version	NM_009604.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, gamma polypeptide (Chrng), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009604.3

ORF Insert Sequence:

ATGCAAGGGG GCCAGAGACC TCAGCTCCTC TTGCTGCTGT TGGCTGTCTG CCTGGGGGCC 
CAGAGCCGCA ACCAAGAGGA GCGTCTGCTT GCGGACCTGA TGCGAAACTA CGACCCCCAC 
CTGCGGCCGG CTGAGCGCGA CTCAGATGTG GTCAATGTCA GCCTGAAGCT TACCTTGACC 
AACCTCATCT CCCTGAATGA ACGAGAGGAG GCCCTCACAA CTAACGTCTG GATAGAGATG 
CAATGGTGCG ACTATCGCCT GCGCTGGGAC CCAAAAGACT ACGAAGGCCT GTGGATATTG 
AGGGTGCCAT CTACTATGGT CTGGCGGCCA GATATCGTCC TGGAGAACAA TGTGGACGGT 
GTCTTCGAGG TGGCTCTCTA CTGCAATGTC CTCGTGTCCC CCGACGGTTG TATCTACTGG 
CTGCCGCCTG CCATCTTCCG CTCCTCCTGC TCCATCTCTG TCACCTACTT CCCCTTCGAT 
TGGCAGAACT GTTCCCTCGT CTTCCAATCC CAGACTTACA GCACCAGTGA GATCAACTTG 
CAGCTGAGCC AGGAGGATGG GCAAGCCATT GAGTGGATCT TCATTGACCC GGAGGCTTTC 
ACAGAGAATG GGGAGTGGGC CATCCGGCAC CGACCGGCTA AAATGCTCCT GGACTCCGTG 
GCTCCTGCAG AGGAGGCGGG CCACCAGAAG GTGGTGTTCT ACCTGCTTAT CCAGCGCAAG 
CCCCTCTTCT ACGTCATCAA CATCATCGCC CCCTGTGTGC TCATCTCCTC AGTCGCCATC 
CTCATCTACT TCCTTCCTGC TAAGGCGGGC GGGCAGAAAT GCACAGTGGC CACCAACGTG 
CTCCTGGCCC AGACTGTCTT CCTTTTCCTT GTGGCTAAGA AGGTGCCTGA GACCTCCCAG 
GCAGTGCCAC TCATCAGCAA GTACCTGACC TTCCTCATGG TGGTGACCAT CCTCATCGTC 
GTGAACTCTG TGGTCGTGCT CAATGTGTCC TTGCGGTCCC CCCACACACA CTCCATGGCC 
CGTGGGGTCC GCAAGGTGTT CCTGAGGCTC CTGCCCCAGC TGTTACGGAT GCATGTGCGC 
CCACTAGCTC CAGCTGCTGT CCAGGATGCC CGGTTCCGAC TCCAGAATGG CTCTTCCTCA 
GGGTGGCCCA TCATGGCTCG AGAGGAAGGG GACCTCTGTC TGCCTCGAAG CGAACTCCTC 
TTTAGGCAAA GGCAGCGCAA TGGATTAGTG CAGGCAGTAT TGGAGAAGCT AGAGAATGGT 
CCAGAAGTGA GGCAGAGCCA GGAGTTCTGT GGCAGCCTGA AGCAAGCCTC CCCAGCCATC 
CAGGCCTGTG TGGATGCCTG TAACCTCATG GCTCGTGCCC GACGCCAGCA GAGTCACTTT 
GACAGTGGGA ACGAGGAGTG GTTGCTGGTG GGCCGAGTGC TGGACCGAGT CTGCTTCCTA 
GCCATGCTCT CCCTCTTCAT CTGTGGCACT GCTGGCATCT TCCTCATGGC CCACTACAAC 
CAAGTGCCTG ACCTGCCGTT CCCCGGAGAC CCCCGCCCCT ACCTGCCTTT GCCAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrng ( XM_006529078.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529141.1 Mus musculus cholinergic receptor, nicotinic, gamma polypeptide (Chrng), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu27865
Clone ID Related Accession (Same CDS sequence)	XM_006529078.3
Accession Version	XM_006529078.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	acetylcholine receptor subunit gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529078.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTC TGGGGGTTAC CTGTACTCTC CCTAGCCCCA GCACCCTCCC CGCCCCCAGT 
CTCCACCTTA TCCCTCTGTG CCTCCTCCTC CTCTGGAGCG AACCCCCTCC TTCTGTTGCA 
GGGGCCCAGA GCCGCAACCA AGAGGAGCGT CTGCTTGCGG ACCTGATGCG AAACTACGAC 
CCCCACCTGC GGCCGGCTGA GCGCGACTCA GATGTGGTCA ATGTCAGCCT GAAGCTTACC 
TTGACCAACC TCATCTCCCT GAATGAACGA GAGGAGGCCC TCACAACTAA CGTCTGGATA 
GAGATGCAAT GGTGCGACTA TCGCCTGCGC TGGGACCCAA AAGACTACGA AGGCCTGTGG 
ATATTGAGGG TGCCATCTAC TATGGTCTGG CGGCCAGATA TCGTCCTGGA GAACAATGTG 
GACGGTGTCT TCGAGGTGGC TCTCTACTGC AATGTCCTCG TGTCCCCCGA CGGTTGTATC 
TACTGGCTGC CGCCTGCCAT CTTCCGCTCC TCCTGCTCCA TCTCTGTCAC CTACTTCCCC 
TTCGATTGGC AGAACTGTTC CCTCGTCTTC CAATCCCAGA CTTACAGCAC CAGTGAGATC 
AACTTGCAGC TGAGCCAGGA GGATGGGCAA GCCATTGAGT GGATCTTCAT TGACCCGGAG 
GCTTTCACAG AGAATGGGGA GTGGGCCATC CGGCACCGAC CGGCTAAAAT GCTCCTGGAC 
TCCGTGGCTC CTGCAGAGGA GGCGGGCCAC CAGAAGGTGG TGTTCTACCT GCTTATCCAG 
CGCAAGCCCC TCTTCTACGT CATCAACATC ATCGCCCCCT GTGTGCTCAT CTCCTCAGTC 
GCCATCCTCA TCTACTTCCT TCCTGCTAAG GCGGGCGGGC AGAAATGCAC AGTGGCCACC 
AACGTGCTCC TGGCCCAGAC TGTCTTCCTT TTCCTTGTGG CTAAGAAGGT GCCTGAGACC 
TCCCAGGCAG TGCCACTCAT CAGCAAGTAC CTGACCTTCC TCATGGTGGT GACCATCCTC 
ATCGTCGTGA ACTCTGTGGT CGTGCTCAAT GTGTCCTTGC GGTCCCCCCA CACACACTCC 
ATGGCCCGTG GGGTCCGCAA GGTGTTCCTG AGGCTCCTGC CCCAGCTGTT ACGGATGCAT 
GTGCGCCCAC TAGCTCCAGC TGCTGTCCAG GATGCCCGGT TCCGACTCCA GAATGGCTCT 
TCCTCAGGGT GGCCCATCAT GGCTCGAGAG GAAGGGGACC TCTGTCTGCC TCGAAGCGAA 
CTCCTCTTTA GGCAAAGGCA GCGCAATGGA TTAGTGCAGG CAGTATTGGA GAAGCTAGAG 
AATGGTCCAG AAGTGAGGCA GAGCCAGGAG TTCTGTGGCA GCCTGAAGCA AGCCTCCCCA 
GCCATCCAGG CCTGTGTGGA TGCCTGTAAC CTCATGGCTC GTGCCCGACG CCAGCAGAGT 
CACTTTGACA GTGGGAACGA GGAGTGGTTG CTGGTGGGCC GAGTGCTGGA CCGAGTCTGC 
TTCCTAGCCA TGCTCTCCCT CTTCATCTGT GGCACTGCTG GCATCTTCCT CATGGCCCAC 
TACAACCAAG TGCCTGACCT GCCGTTCCCC GGAGACCCCC GCCCCTACCT GCCTTTGCCA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529141.1
CDS	69..1694
Translation	MEGLGVTCTLPSPSTLPAPSLHLIPLCLLLLWSEPPPSVAGAQSRNQEERLLADLMRNYDPHLRPAERDSDVVNVSLKLTLTNLISLNEREEALTTNVWIEMQWCDYRLRWDPKDYEGLWILRVPSTMVWRPDIVLENNVDGVFEVALYCNVLVSPDGCIYWLPPAIFRSSCSISVTYFPFDWQNCSLVFQSQTYSTSEINLQLSQEDGQAIEWIFIDPEAFTENGEWAIRHRPAKMLLDSVAPAEEAGHQKVVFYLLIQRKPLFYVINIIAPCVLISSVAILIYFLPAKAGGQKCTVATNVLLAQTVFLFLVAKKVPETSQAVPLISKYLTFLMVVTILIVVNSVVVLNVSLRSPHTHSMARGVRKVFLRLLPQLLRMHVRPLAPAAVQDARFRLQNGSSSGWPIMAREEGDLCLPRSELLFRQRQRNGLVQAVLEKLENGPEVRQSQEFCGSLKQASPAIQACVDACNLMARARRQQSHFDSGNEEWLLVGRVLDRVCFLAMLSLFICGTAGIFLMAHYNQVPDLPFPGDPRPYLPLPD

Target ORF information:

RefSeq Version	XM_006529078.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, gamma polypeptide (Chrng), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529078.3

ORF Insert Sequence:

ATGGAGGGTC TGGGGGTTAC CTGTACTCTC CCTAGCCCCA GCACCCTCCC CGCCCCCAGT 
CTCCACCTTA TCCCTCTGTG CCTCCTCCTC CTCTGGAGCG AACCCCCTCC TTCTGTTGCA 
GGGGCCCAGA GCCGCAACCA AGAGGAGCGT CTGCTTGCGG ACCTGATGCG AAACTACGAC 
CCCCACCTGC GGCCGGCTGA GCGCGACTCA GATGTGGTCA ATGTCAGCCT GAAGCTTACC 
TTGACCAACC TCATCTCCCT GAATGAACGA GAGGAGGCCC TCACAACTAA CGTCTGGATA 
GAGATGCAAT GGTGCGACTA TCGCCTGCGC TGGGACCCAA AAGACTACGA AGGCCTGTGG 
ATATTGAGGG TGCCATCTAC TATGGTCTGG CGGCCAGATA TCGTCCTGGA GAACAATGTG 
GACGGTGTCT TCGAGGTGGC TCTCTACTGC AATGTCCTCG TGTCCCCCGA CGGTTGTATC 
TACTGGCTGC CGCCTGCCAT CTTCCGCTCC TCCTGCTCCA TCTCTGTCAC CTACTTCCCC 
TTCGATTGGC AGAACTGTTC CCTCGTCTTC CAATCCCAGA CTTACAGCAC CAGTGAGATC 
AACTTGCAGC TGAGCCAGGA GGATGGGCAA GCCATTGAGT GGATCTTCAT TGACCCGGAG 
GCTTTCACAG AGAATGGGGA GTGGGCCATC CGGCACCGAC CGGCTAAAAT GCTCCTGGAC 
TCCGTGGCTC CTGCAGAGGA GGCGGGCCAC CAGAAGGTGG TGTTCTACCT GCTTATCCAG 
CGCAAGCCCC TCTTCTACGT CATCAACATC ATCGCCCCCT GTGTGCTCAT CTCCTCAGTC 
GCCATCCTCA TCTACTTCCT TCCTGCTAAG GCGGGCGGGC AGAAATGCAC AGTGGCCACC 
AACGTGCTCC TGGCCCAGAC TGTCTTCCTT TTCCTTGTGG CTAAGAAGGT GCCTGAGACC 
TCCCAGGCAG TGCCACTCAT CAGCAAGTAC CTGACCTTCC TCATGGTGGT GACCATCCTC 
ATCGTCGTGA ACTCTGTGGT CGTGCTCAAT GTGTCCTTGC GGTCCCCCCA CACACACTCC 
ATGGCCCGTG GGGTCCGCAA GGTGTTCCTG AGGCTCCTGC CCCAGCTGTT ACGGATGCAT 
GTGCGCCCAC TAGCTCCAGC TGCTGTCCAG GATGCCCGGT TCCGACTCCA GAATGGCTCT 
TCCTCAGGGT GGCCCATCAT GGCTCGAGAG GAAGGGGACC TCTGTCTGCC TCGAAGCGAA 
CTCCTCTTTA GGCAAAGGCA GCGCAATGGA TTAGTGCAGG CAGTATTGGA GAAGCTAGAG 
AATGGTCCAG AAGTGAGGCA GAGCCAGGAG TTCTGTGGCA GCCTGAAGCA AGCCTCCCCA 
GCCATCCAGG CCTGTGTGGA TGCCTGTAAC CTCATGGCTC GTGCCCGACG CCAGCAGAGT 
CACTTTGACA GTGGGAACGA GGAGTGGTTG CTGGTGGGCC GAGTGCTGGA CCGAGTCTGC 
TTCCTAGCCA TGCTCTCCCT CTTCATCTGT GGCACTGCTG GCATCTTCCT CATGGCCCAC 
TACAACCAAG TGCCTGACCT GCCGTTCCCC GGAGACCCCC GCCCCTACCT GCCTTTGCCA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assessment of the expression and role of the ?1-nAChR subunit in efferent cholinergic function during the development of the mammalian cochlea.
Journal of neurophysiology116(2)479-92(2016 Aug)
Roux I,Wu JS,McIntosh JM,Glowatzki E

Severe muscle wasting and denervation in mice lacking the RNA-binding protein ZFP106.
Proceedings of the National Academy of Sciences of the United States of America113(31)E4494-503(2016 08)
Anderson DM,Cannavino J,Li H,Anderson KM,Nelson BR,McAnally J,Bezprozvannaya S,Liu Y,Lin W,Liu N,Bassel-Duby R,Olson EN

Neuromuscular junction immaturity and muscle atrophy are hallmarks of the ColQ-deficient mouse, a model of congenital myasthenic syndrome with acetylcholinesterase deficiency.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(6)2382-99(2016 06)
Sigoillot SM,Bourgeois F,Karmouch J,Molg? J,Dobbertin A,Chevalier C,Houlgatte R,L?ger J,Legay C

Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle.
Skeletal muscle6(1)30(2016)
Sakakibara I,Wurmser M,Dos Santos M,Santolini M,Ducommun S,Davaze R,Guernec A,Sakamoto K,Maire P

Motor and Sensory Deficits in the teetering Mice Result from Mutation of the ESCRT Component HGS.
PLoS genetics11(6)e1005290(2015 Jun)
Watson JA,Bhattacharyya BJ,Vaden JH,Wilson JA,Icyuz M,Howard AD,Phillips E,DeSilva TM,Siegal GP,Bean AJ,King GD,Phillips SE,Miller RJ,Wilson SM

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Chrng cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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