Chrne cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrne
Entrez Gene ID	11448
Full Name	cholinergic receptor, nicotinic, epsilon polypeptide
Synonyms	AChrepsilon,Acre,nAChRE
General protein information	Preferred Names cholinergic receptor, nicotinic, epsilon polypeptide Names acetylcholine receptor subunit epsilon AchR epsilon subunit nicotinic acetylcholine receptor epsilon subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B3\|11 43.14 cM
Summary	This gene encodes the epsilon subunit of the muscle-derived nicotinic acetylcholine receptor, a pentameric neurotransmitter receptor and member of the ligand-gated ion channel superfamily. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. In mice, deficiency of this gene can lead to a decline in the number of nicotinic acetylcholine receptors at neuromuscular junctions and causes progressive muscle weakness, atrophy and premature death. Mutations in this gene serve as a pathophysiological model for human congenital myasthenia. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Nov 2012].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532006.4	XP_006532069.1	acetylcholine receptor subunit epsilon isoform X1
XM_006532007.4	XP_006532070.1	acetylcholine receptor subunit epsilon isoform X2
XM_006532006.3	XP_006532069.1	acetylcholine receptor subunit epsilon isoform X1
NM_009603.1	NP_033733.1	acetylcholine receptor subunit epsilon precursor
XM_006532007.3	XP_006532070.1	acetylcholine receptor subunit epsilon isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHRNE	NP_000071.1
Rattus norvegicus (Norway rat)	Chrne	NP_058890.1
Xenopus tropicalis (tropical clawed frog)	chrne	XP_002941867.2
Pan troglodytes (chimpanzee)	CHRNE	XP_523556.4
Canis lupus familiaris (dog)	CHRNE	XP_536608.3
Bos taurus (cattle)	CHRNE	NP_776697.1
	CHRNE	XP_001097094.1
Mus musculus (house mouse)	Chrne	NP_033733.1

Gene Ontology
Bibliography

Related articles in PubMed

Lack of Fgf18 causes abnormal clustering of motor nerve terminals at the neuromuscular junction with reduced acetylcholine receptor clusters.
Ito K, Ohkawara B, Yagi H, Nakashima H, Tsushima M, Ota K, Konishi H, Masuda A, Imagama S, Kiyama H, Ishiguro N, Ohno K
Scientific reports8(1)434(2018 Jan)

Neuromuscular junction immaturity and muscle atrophy are hallmarks of the ColQ-deficient mouse, a model of congenital myasthenic syndrome with acetylcholinesterase deficiency.
Sigoillot SM, Bourgeois F, Karmouch J, Molg? J, Dobbertin A, Chevalier C, Houlgatte R, L?ger J, Legay C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(6)2382-99(2016 06)

Motor and Sensory Deficits in the teetering Mice Result from Mutation of the ESCRT Component HGS.
Watson JA, Bhattacharyya BJ, Vaden JH, Wilson JA, Icyuz M, Howard AD, Phillips E, DeSilva TM, Siegal GP, Bean AJ, King GD, Phillips SE, Miller RJ, Wilson SM
PLoS genetics11(6)e1005290(2015 Jun)

Deficient development and maintenance of postsynaptic specializations in mutant mice lacking an 'adult' acetylcholine receptor subunit.
Missias AC, Mudd J, Cunningham JM, Steinbach JH, Merlie JP, Sanes JR
Development (Cambridge, England)124(24)5075-86(1997 Dec)

Acetylcholine receptor epsilon-subunit deletion causes muscle weakness and atrophy in juvenile and adult mice.
Witzemann V, Schwarz H, Koenen M, Berberich C, Villarroel A, Wernig A, Brenner HR, Sakmann B
Proceedings of the National Academy of Sciences of the United States of America93(23)13286-91(1996 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a pattern of histone modifications associated with transcriptional activation is targeted to the AChR epsilon subunit locus in myotubes prior to stimulation
Title: Chromatin modifications that support acetylcholine receptor gene activation are established during muscle cell determination and differentiation.

analysis of symmetry at the extracellular domain-transmembrane domain interface in acetylcholine receptor channel gating
Title: Subunit symmetry at the extracellular domain-transmembrane domain interface in acetylcholine receptor channel gating.

The local anaesthetics proadifen and adiphenine inhibit nicotinic receptors by different molecular mechanisms.
Title: The local anaesthetics proadifen and adiphenine inhibit nicotinic receptors by different molecular mechanisms.

In L221F, T264P, L269F mutants the peak-current amplitude decreases along trains of nearly saturating ACh pulses delivered at physiologically relevant frequencies, consistent with enhanced entry into desensitization in congenital myasthenic syndrome.
Title: Decremental response to high-frequency trains of acetylcholine pulses but unaltered fractional Ca2+ currents in a panel of "slow-channel syndrome" nicotinic receptor mutants.

CDK2 is an intermediate molecule that integrates neuregulin-activated signals from both the MAPK and PI3K pathways to AChRepsilon expression
Title: Novel role for cyclin-dependent kinase 2 in neuregulin-induced acetylcholine receptor epsilon subunit expression in differentiated myotubes.

The following Chrne gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrne cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15722	NM_009603.1 Latest version!	Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$286.30 $409.00
OMu45418	XM_006532006.4 Latest version!	Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu45419	XM_006532007.4 Latest version!	Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu45418	XM_006532006.3	Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu45419	XM_006532007.3	Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrne ( NM_009603.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033733.1 Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), mRNA.

Price & Availability↑

CloneID	OMu15722
Clone ID Related Accession (Same CDS sequence)	NM_009603.1
Accession Version	NM_009603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	acetylcholine receptor subunit epsilon precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X55718.1. On Jul 25, 2007 this sequence version replaced XM_001471640.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X55718.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGGG CTCTGCTTGG TGCCCTGCTT CTCCTGACAC TCTTTGGCAG AAGCCAGGGA 
AAGAATGAAG AGCTTAGCCT GTATCACCAT CTCTTCGACA ATTATGATCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ACGAGAAAGA AGAAACTCTG ACCACCAGTG TCTGGATTGG CATTGACTGG 
CACGACTATC GGCTCAACTA CAGCAAGGAC GATTTTGCAG GTGTAGGAAT CCTCCGGGTC 
CCTTCAGAAC ATGTATGGCT GCCAGAGATT GTTCTAGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACAGCAA TGTTCTAGTC TATGAGGGAG GCTATGTGAG CTGGTTGCCC 
CCAGCCATCT ACCGCAGCAC CTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG CTCCCAGACC TACAATGCTG AGGAGGTGGA GTTCATCTTT 
GCCGTGGATG ACGACGGCAA TACCATCAAC AAGATTGACA TTGACACGGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT AGACTACTGC CCAGGCATGA TTCGCCGCTA TGAGGGAGGT 
TCCACAGAAG GTCCTGGAGA AACTGACGTC ATCTATACGC TCATCATCCG CCGGAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGCGTGCTCA TTTCTGGCTT GGTGCTGCTC 
GCTTACTTCC TGCCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTTCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCACTGC TGGGCAGGTA TCTTATATTC GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG AGGACGCCAA CGACTCATGC TACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTGCTG CCGCGTCTCC TGGGCTCGAG CCCACCCCCA 
GAGGATCCCC GAACTGCCTC ACCAGCGAGG CGTGCCTCGT CTGTGGGCAT TCTGCTCAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG TCAGAGGCAT 
CGGCACGGAA CTTGGACCGC AGCCCTCTGC CAGAACCTGG GTGCTGCAGC CCCAGAAATC 
CGCTGCTGTG TGGATGCTGT GAACTTTGTG GCTGAGAGCA CAAGAGACCA GGAAGCCACT 
GGAGAGGAAC TGTCCGACTG GGTGCGTATG GGGAAGGCCC TGGACAATGT CTGTTTTTGG 
GCAGCTTTGG TGCTCTTCAG CGTTGGTTCT ACTCTCATCT TCCTTGGGGG TTACTTCAAC 
CAAGTTCCTG ATCTCCCTTA CCCACCGTGC ATCCAACCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033733.1
CDS	20..1501
Translation	MAGALLGALLLLTLFGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIDWHDYRLNYSKDDFAGVGILRVPSEHVWLPEIVLENNIDGQFGVAYDSNVLVYEGGYVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNTINKIDIDTAAFTENGEWAIDYCPGMIRRYEGGSTEGPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELLPRLLGSSPPPEDPRTASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTAALCQNLGAAAPEIRCCVDAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDLPYPPCIQP

Target ORF information:

RefSeq Version	NM_009603.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009603.1

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTTGG TGCCCTGCTT CTCCTGACAC TCTTTGGCAG AAGCCAGGGA 
AAGAATGAAG AGCTTAGCCT GTATCACCAT CTCTTCGACA ATTATGATCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ACGAGAAAGA AGAAACTCTG ACCACCAGTG TCTGGATTGG CATTGACTGG 
CACGACTATC GGCTCAACTA CAGCAAGGAC GATTTTGCAG GTGTAGGAAT CCTCCGGGTC 
CCTTCAGAAC ATGTATGGCT GCCAGAGATT GTTCTAGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACAGCAA TGTTCTAGTC TATGAGGGAG GCTATGTGAG CTGGTTGCCC 
CCAGCCATCT ACCGCAGCAC CTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG CTCCCAGACC TACAATGCTG AGGAGGTGGA GTTCATCTTT 
GCCGTGGATG ACGACGGCAA TACCATCAAC AAGATTGACA TTGACACGGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT AGACTACTGC CCAGGCATGA TTCGCCGCTA TGAGGGAGGT 
TCCACAGAAG GTCCTGGAGA AACTGACGTC ATCTATACGC TCATCATCCG CCGGAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGCGTGCTCA TTTCTGGCTT GGTGCTGCTC 
GCTTACTTCC TGCCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTTCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCACTGC TGGGCAGGTA TCTTATATTC GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG AGGACGCCAA CGACTCATGC TACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTGCTG CCGCGTCTCC TGGGCTCGAG CCCACCCCCA 
GAGGATCCCC GAACTGCCTC ACCAGCGAGG CGTGCCTCGT CTGTGGGCAT TCTGCTCAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG TCAGAGGCAT 
CGGCACGGAA CTTGGACCGC AGCCCTCTGC CAGAACCTGG GTGCTGCAGC CCCAGAAATC 
CGCTGCTGTG TGGATGCTGT GAACTTTGTG GCTGAGAGCA CAAGAGACCA GGAAGCCACT 
GGAGAGGAAC TGTCCGACTG GGTGCGTATG GGGAAGGCCC TGGACAATGT CTGTTTTTGG 
GCAGCTTTGG TGCTCTTCAG CGTTGGTTCT ACTCTCATCT TCCTTGGGGG TTACTTCAAC 
CAAGTTCCTG ATCTCCCTTA CCCACCGTGC ATCCAACCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrne ( XM_006532006.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532069.1 Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45418
Clone ID Related Accession (Same CDS sequence)	XM_006532006.4 , XM_006532006.3
Accession Version	XM_006532006.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	acetylcholine receptor subunit epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CTCTGCTTGG TGCCCTGCTT CTCCTGACAC TCTTTGGCAG AAGCCAGGGA 
AAGAATGAAG AGCTTAGCCT GTATCACCAT CTCTTCGACA ATTATGATCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ACGAGAAAGA AGAAACTCTG ACCACCAGTG TCTGGATTGG CATTGACTGG 
CACGACTATC GGCTCAACTA CAGCAAGGAC GATTTTGCAG GTGTAGGAAT CCTCCGGGTC 
CCTTCAGAAC ATGTATGGCT GCCAGAGATT GTTCTAGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACAGCAA TGTTCTAGTC TATGAGGGAG GCTATGTGAG CTGGTTGCCC 
CCAGCCATCT ACCGCAGCAC CTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG CTCCCAGACC TACAATGCTG AGGAGGTGGA GTTCATCTTT 
GCCGTGGATG ACGACGGCAA TACCATCAAC AAGATTGACA TTGACACGGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT AGACTACTGC CCAGGCATGA TTCGCCGCTA TGAGGGAGGT 
TCCACAGAAG GTCCTGGAGA AACTGACGTC ATCTATACGC TCATCATCCG CCGGAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGCGTGCTCA TTTCTGGCTT GGTGCTGCTC 
GCTTACTTCC TGCCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTTCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCACTGC TGGGCAGGTA TCTTATATTC GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG AGGACGCCAA CGACTCATGC TACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTGCTG CCGCGTCTCC TGGGCTCGAG CCCACCCCCA 
GAGGATCCCC GAACTGCCTC ACCAGCGAGG CGTGCCTCGT CTGTGGGCAT TCTGCTCAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG TCAGAGGCAT 
CGGCACGGAA CTTGGACCGC AGCAGCCCTC TGCCAGAACC TGGGTGCTGC AGCCCCAGAA 
ATCCGCTGCT GTGTGGATGC TGTGAACTTT GTGGCTGAGA GCACAAGAGA CCAGGAAGCC 
ACTGGAGAGG AACTGTCCGA CTGGGTGCGT ATGGGGAAGG CCCTGGACAA TGTCTGTTTT 
TGGGCAGCTT TGGTGCTCTT CAGCGTTGGT TCTACTCTCA TCTTCCTTGG GGGTTACTTC 
AACCAAGTTC CTGATCTCCC TTACCCACCG TGCATCCAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532069.1
CDS	191..1675
Translation	MAGALLGALLLLTLFGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIDWHDYRLNYSKDDFAGVGILRVPSEHVWLPEIVLENNIDGQFGVAYDSNVLVYEGGYVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNTINKIDIDTAAFTENGEWAIDYCPGMIRRYEGGSTEGPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELLPRLLGSSPPPEDPRTASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTAAALCQNLGAAAPEIRCCVDAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDLPYPPCIQP

Target ORF information:

RefSeq Version	XM_006532006.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532006.3

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTTGG TGCCCTGCTT CTCCTGACAC TCTTTGGCAG AAGCCAGGGA 
AAGAATGAAG AGCTTAGCCT GTATCACCAT CTCTTCGACA ATTATGATCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ACGAGAAAGA AGAAACTCTG ACCACCAGTG TCTGGATTGG CATTGACTGG 
CACGACTATC GGCTCAACTA CAGCAAGGAC GATTTTGCAG GTGTAGGAAT CCTCCGGGTC 
CCTTCAGAAC ATGTATGGCT GCCAGAGATT GTTCTAGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACAGCAA TGTTCTAGTC TATGAGGGAG GCTATGTGAG CTGGTTGCCC 
CCAGCCATCT ACCGCAGCAC CTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG CTCCCAGACC TACAATGCTG AGGAGGTGGA GTTCATCTTT 
GCCGTGGATG ACGACGGCAA TACCATCAAC AAGATTGACA TTGACACGGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT AGACTACTGC CCAGGCATGA TTCGCCGCTA TGAGGGAGGT 
TCCACAGAAG GTCCTGGAGA AACTGACGTC ATCTATACGC TCATCATCCG CCGGAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGCGTGCTCA TTTCTGGCTT GGTGCTGCTC 
GCTTACTTCC TGCCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTTCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCACTGC TGGGCAGGTA TCTTATATTC GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG AGGACGCCAA CGACTCATGC TACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTGCTG CCGCGTCTCC TGGGCTCGAG CCCACCCCCA 
GAGGATCCCC GAACTGCCTC ACCAGCGAGG CGTGCCTCGT CTGTGGGCAT TCTGCTCAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG TCAGAGGCAT 
CGGCACGGAA CTTGGACCGC AGCAGCCCTC TGCCAGAACC TGGGTGCTGC AGCCCCAGAA 
ATCCGCTGCT GTGTGGATGC TGTGAACTTT GTGGCTGAGA GCACAAGAGA CCAGGAAGCC 
ACTGGAGAGG AACTGTCCGA CTGGGTGCGT ATGGGGAAGG CCCTGGACAA TGTCTGTTTT 
TGGGCAGCTT TGGTGCTCTT CAGCGTTGGT TCTACTCTCA TCTTCCTTGG GGGTTACTTC 
AACCAAGTTC CTGATCTCCC TTACCCACCG TGCATCCAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrne ( XM_006532007.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532070.1 Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45419
Clone ID Related Accession (Same CDS sequence)	XM_006532007.4 , XM_006532007.3
Accession Version	XM_006532007.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	acetylcholine receptor subunit epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532007.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CTCTGCTTGG TGCCCTGCTT CTCCTGACAC TCTTTGGCAG AAGCCAGGGA 
AAGAATGAAG AGCTTAGCCT GTATCACCAT CTCTTCGACA ATTATGATCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ACGAGAAAGA AGAAACTCTG ACCACCAGTG TCTGGATTGG CATTGACTGG 
CACGACTATC GGCTCAACTA CAGCAAGGAC GATTTTGCAG GTGTAGGAAT CCTCCGGGTC 
CCTTCAGAAC ATGTATGGCT GCCAGAGATT GTTCTAGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACAGCAA TGTTCTAGTC TATGAGGGAG GCTATGTGAG CTGGTTGCCC 
CCAGCCATCT ACCGCAGCAC CTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG CTCCCAGACC TACAATGCTG AGGAGGTGGA GTTCATCTTT 
GCCGTGGATG ACGACGGCAA TACCATCAAC AAGATTGACA TTGACACGGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT AGACTACTGC CCAGGCATGA TTCGCCGCTA TGAGGGAGGT 
TCCACAGAAG GTCCTGGAGA AACTGACGTC ATCTATACGC TCATCATCCG CCGGAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGCGTGCTCA TTTCTGGCTT GGTGCTGCTC 
GCTTACTTCC TGCCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTTCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCACTGC TGGGCAGGTA TCTTATATTC GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG AGGACGCCAA CGACTCATGC TACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTGCTG CCGCGTCTCC TGGGCTCGAG CCCACCCCCA 
GAGGATCCCC GAACTGCCTC ACCAGCGAGG CGTGCCTCGT CTGTGGGCAT TCTGCTCAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG TCAGAGGCAT 
CGGCACGGAA CTTGGACCGG AACTGTCCGA CTGGGTGCGT ATGGGGAAGG CCCTGGACAA 
TGTCTGTTTT TGGGCAGCTT TGGTGCTCTT CAGCGTTGGT TCTACTCTCA TCTTCCTTGG 
GGGTTACTTC AACCAAGTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532070.1
CDS	191..1534
Translation	MAGALLGALLLLTLFGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIDWHDYRLNYSKDDFAGVGILRVPSEHVWLPEIVLENNIDGQFGVAYDSNVLVYEGGYVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNTINKIDIDTAAFTENGEWAIDYCPGMIRRYEGGSTEGPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELLPRLLGSSPPPEDPRTASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTGTVRLGAYGEGPGQCLFLGSFGALQRWFYSHLPWGLLQPSS

Target ORF information:

RefSeq Version	XM_006532007.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532007.3

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTTGG TGCCCTGCTT CTCCTGACAC TCTTTGGCAG AAGCCAGGGA 
AAGAATGAAG AGCTTAGCCT GTATCACCAT CTCTTCGACA ATTATGATCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ACGAGAAAGA AGAAACTCTG ACCACCAGTG TCTGGATTGG CATTGACTGG 
CACGACTATC GGCTCAACTA CAGCAAGGAC GATTTTGCAG GTGTAGGAAT CCTCCGGGTC 
CCTTCAGAAC ATGTATGGCT GCCAGAGATT GTTCTAGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACAGCAA TGTTCTAGTC TATGAGGGAG GCTATGTGAG CTGGTTGCCC 
CCAGCCATCT ACCGCAGCAC CTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG CTCCCAGACC TACAATGCTG AGGAGGTGGA GTTCATCTTT 
GCCGTGGATG ACGACGGCAA TACCATCAAC AAGATTGACA TTGACACGGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT AGACTACTGC CCAGGCATGA TTCGCCGCTA TGAGGGAGGT 
TCCACAGAAG GTCCTGGAGA AACTGACGTC ATCTATACGC TCATCATCCG CCGGAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGCGTGCTCA TTTCTGGCTT GGTGCTGCTC 
GCTTACTTCC TGCCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTTCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCACTGC TGGGCAGGTA TCTTATATTC GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG AGGACGCCAA CGACTCATGC TACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTGCTG CCGCGTCTCC TGGGCTCGAG CCCACCCCCA 
GAGGATCCCC GAACTGCCTC ACCAGCGAGG CGTGCCTCGT CTGTGGGCAT TCTGCTCAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG TCAGAGGCAT 
CGGCACGGAA CTTGGACCGG AACTGTCCGA CTGGGTGCGT ATGGGGAAGG CCCTGGACAA 
TGTCTGTTTT TGGGCAGCTT TGGTGCTCTT CAGCGTTGGT TCTACTCTCA TCTTCCTTGG 
GGGTTACTTC AACCAAGTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrne ( XM_006532006.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532069.1 Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45418
Clone ID Related Accession (Same CDS sequence)	XM_006532006.4 , XM_006532006.3
Accession Version	XM_006532006.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	acetylcholine receptor subunit epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532006.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CTCTGCTTGG TGCCCTGCTT CTCCTGACAC TCTTTGGCAG AAGCCAGGGA 
AAGAATGAAG AGCTTAGCCT GTATCACCAT CTCTTCGACA ATTATGATCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ACGAGAAAGA AGAAACTCTG ACCACCAGTG TCTGGATTGG CATTGACTGG 
CACGACTATC GGCTCAACTA CAGCAAGGAC GATTTTGCAG GTGTAGGAAT CCTCCGGGTC 
CCTTCAGAAC ATGTATGGCT GCCAGAGATT GTTCTAGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACAGCAA TGTTCTAGTC TATGAGGGAG GCTATGTGAG CTGGTTGCCC 
CCAGCCATCT ACCGCAGCAC CTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG CTCCCAGACC TACAATGCTG AGGAGGTGGA GTTCATCTTT 
GCCGTGGATG ACGACGGCAA TACCATCAAC AAGATTGACA TTGACACGGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT AGACTACTGC CCAGGCATGA TTCGCCGCTA TGAGGGAGGT 
TCCACAGAAG GTCCTGGAGA AACTGACGTC ATCTATACGC TCATCATCCG CCGGAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGCGTGCTCA TTTCTGGCTT GGTGCTGCTC 
GCTTACTTCC TGCCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTTCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCACTGC TGGGCAGGTA TCTTATATTC GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG AGGACGCCAA CGACTCATGC TACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTGCTG CCGCGTCTCC TGGGCTCGAG CCCACCCCCA 
GAGGATCCCC GAACTGCCTC ACCAGCGAGG CGTGCCTCGT CTGTGGGCAT TCTGCTCAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG TCAGAGGCAT 
CGGCACGGAA CTTGGACCGC AGCAGCCCTC TGCCAGAACC TGGGTGCTGC AGCCCCAGAA 
ATCCGCTGCT GTGTGGATGC TGTGAACTTT GTGGCTGAGA GCACAAGAGA CCAGGAAGCC 
ACTGGAGAGG AACTGTCCGA CTGGGTGCGT ATGGGGAAGG CCCTGGACAA TGTCTGTTTT 
TGGGCAGCTT TGGTGCTCTT CAGCGTTGGT TCTACTCTCA TCTTCCTTGG GGGTTACTTC 
AACCAAGTTC CTGATCTCCC TTACCCACCG TGCATCCAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532069.1
CDS	37..1521
Translation	MAGALLGALLLLTLFGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIDWHDYRLNYSKDDFAGVGILRVPSEHVWLPEIVLENNIDGQFGVAYDSNVLVYEGGYVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNTINKIDIDTAAFTENGEWAIDYCPGMIRRYEGGSTEGPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELLPRLLGSSPPPEDPRTASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTAAALCQNLGAAAPEIRCCVDAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDLPYPPCIQP

Target ORF information:

RefSeq Version	XM_006532006.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532006.4

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTTGG TGCCCTGCTT CTCCTGACAC TCTTTGGCAG AAGCCAGGGA 
AAGAATGAAG AGCTTAGCCT GTATCACCAT CTCTTCGACA ATTATGATCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ACGAGAAAGA AGAAACTCTG ACCACCAGTG TCTGGATTGG CATTGACTGG 
CACGACTATC GGCTCAACTA CAGCAAGGAC GATTTTGCAG GTGTAGGAAT CCTCCGGGTC 
CCTTCAGAAC ATGTATGGCT GCCAGAGATT GTTCTAGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACAGCAA TGTTCTAGTC TATGAGGGAG GCTATGTGAG CTGGTTGCCC 
CCAGCCATCT ACCGCAGCAC CTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG CTCCCAGACC TACAATGCTG AGGAGGTGGA GTTCATCTTT 
GCCGTGGATG ACGACGGCAA TACCATCAAC AAGATTGACA TTGACACGGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT AGACTACTGC CCAGGCATGA TTCGCCGCTA TGAGGGAGGT 
TCCACAGAAG GTCCTGGAGA AACTGACGTC ATCTATACGC TCATCATCCG CCGGAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGCGTGCTCA TTTCTGGCTT GGTGCTGCTC 
GCTTACTTCC TGCCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTTCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCACTGC TGGGCAGGTA TCTTATATTC GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG AGGACGCCAA CGACTCATGC TACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTGCTG CCGCGTCTCC TGGGCTCGAG CCCACCCCCA 
GAGGATCCCC GAACTGCCTC ACCAGCGAGG CGTGCCTCGT CTGTGGGCAT TCTGCTCAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG TCAGAGGCAT 
CGGCACGGAA CTTGGACCGC AGCAGCCCTC TGCCAGAACC TGGGTGCTGC AGCCCCAGAA 
ATCCGCTGCT GTGTGGATGC TGTGAACTTT GTGGCTGAGA GCACAAGAGA CCAGGAAGCC 
ACTGGAGAGG AACTGTCCGA CTGGGTGCGT ATGGGGAAGG CCCTGGACAA TGTCTGTTTT 
TGGGCAGCTT TGGTGCTCTT CAGCGTTGGT TCTACTCTCA TCTTCCTTGG GGGTTACTTC 
AACCAAGTTC CTGATCTCCC TTACCCACCG TGCATCCAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrne ( XM_006532007.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532070.1 Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu45419
Clone ID Related Accession (Same CDS sequence)	XM_006532007.4 , XM_006532007.3
Accession Version	XM_006532007.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	acetylcholine receptor subunit epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532007.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CTCTGCTTGG TGCCCTGCTT CTCCTGACAC TCTTTGGCAG AAGCCAGGGA 
AAGAATGAAG AGCTTAGCCT GTATCACCAT CTCTTCGACA ATTATGATCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ACGAGAAAGA AGAAACTCTG ACCACCAGTG TCTGGATTGG CATTGACTGG 
CACGACTATC GGCTCAACTA CAGCAAGGAC GATTTTGCAG GTGTAGGAAT CCTCCGGGTC 
CCTTCAGAAC ATGTATGGCT GCCAGAGATT GTTCTAGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACAGCAA TGTTCTAGTC TATGAGGGAG GCTATGTGAG CTGGTTGCCC 
CCAGCCATCT ACCGCAGCAC CTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG CTCCCAGACC TACAATGCTG AGGAGGTGGA GTTCATCTTT 
GCCGTGGATG ACGACGGCAA TACCATCAAC AAGATTGACA TTGACACGGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT AGACTACTGC CCAGGCATGA TTCGCCGCTA TGAGGGAGGT 
TCCACAGAAG GTCCTGGAGA AACTGACGTC ATCTATACGC TCATCATCCG CCGGAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGCGTGCTCA TTTCTGGCTT GGTGCTGCTC 
GCTTACTTCC TGCCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTTCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCACTGC TGGGCAGGTA TCTTATATTC GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG AGGACGCCAA CGACTCATGC TACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTGCTG CCGCGTCTCC TGGGCTCGAG CCCACCCCCA 
GAGGATCCCC GAACTGCCTC ACCAGCGAGG CGTGCCTCGT CTGTGGGCAT TCTGCTCAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG TCAGAGGCAT 
CGGCACGGAA CTTGGACCGG AACTGTCCGA CTGGGTGCGT ATGGGGAAGG CCCTGGACAA 
TGTCTGTTTT TGGGCAGCTT TGGTGCTCTT CAGCGTTGGT TCTACTCTCA TCTTCCTTGG 
GGGTTACTTC AACCAAGTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532070.1
CDS	39..1382
Translation	MAGALLGALLLLTLFGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIDWHDYRLNYSKDDFAGVGILRVPSEHVWLPEIVLENNIDGQFGVAYDSNVLVYEGGYVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNTINKIDIDTAAFTENGEWAIDYCPGMIRRYEGGSTEGPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELLPRLLGSSPPPEDPRTASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTGTVRLGAYGEGPGQCLFLGSFGALQRWFYSHLPWGLLQPSS

Target ORF information:

RefSeq Version	XM_006532007.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532007.4

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTTGG TGCCCTGCTT CTCCTGACAC TCTTTGGCAG AAGCCAGGGA 
AAGAATGAAG AGCTTAGCCT GTATCACCAT CTCTTCGACA ATTATGATCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ACGAGAAAGA AGAAACTCTG ACCACCAGTG TCTGGATTGG CATTGACTGG 
CACGACTATC GGCTCAACTA CAGCAAGGAC GATTTTGCAG GTGTAGGAAT CCTCCGGGTC 
CCTTCAGAAC ATGTATGGCT GCCAGAGATT GTTCTAGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACAGCAA TGTTCTAGTC TATGAGGGAG GCTATGTGAG CTGGTTGCCC 
CCAGCCATCT ACCGCAGCAC CTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG CTCCCAGACC TACAATGCTG AGGAGGTGGA GTTCATCTTT 
GCCGTGGATG ACGACGGCAA TACCATCAAC AAGATTGACA TTGACACGGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT AGACTACTGC CCAGGCATGA TTCGCCGCTA TGAGGGAGGT 
TCCACAGAAG GTCCTGGAGA AACTGACGTC ATCTATACGC TCATCATCCG CCGGAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGCGTGCTCA TTTCTGGCTT GGTGCTGCTC 
GCTTACTTCC TGCCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTTCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCACTGC TGGGCAGGTA TCTTATATTC GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG AGGACGCCAA CGACTCATGC TACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTGCTG CCGCGTCTCC TGGGCTCGAG CCCACCCCCA 
GAGGATCCCC GAACTGCCTC ACCAGCGAGG CGTGCCTCGT CTGTGGGCAT TCTGCTCAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG TCAGAGGCAT 
CGGCACGGAA CTTGGACCGG AACTGTCCGA CTGGGTGCGT ATGGGGAAGG CCCTGGACAA 
TGTCTGTTTT TGGGCAGCTT TGGTGCTCTT CAGCGTTGGT TCTACTCTCA TCTTCCTTGG 
GGGTTACTTC AACCAAGTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lack of Fgf18 causes abnormal clustering of motor nerve terminals at the neuromuscular junction with reduced acetylcholine receptor clusters.
Scientific reports8(1)434(2018 Jan)
Ito K,Ohkawara B,Yagi H,Nakashima H,Tsushima M,Ota K,Konishi H,Masuda A,Imagama S,Kiyama H,Ishiguro N,Ohno K

Neuromuscular junction immaturity and muscle atrophy are hallmarks of the ColQ-deficient mouse, a model of congenital myasthenic syndrome with acetylcholinesterase deficiency.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(6)2382-99(2016 06)
Sigoillot SM,Bourgeois F,Karmouch J,Molg? J,Dobbertin A,Chevalier C,Houlgatte R,L?ger J,Legay C

Motor and Sensory Deficits in the teetering Mice Result from Mutation of the ESCRT Component HGS.
PLoS genetics11(6)e1005290(2015 Jun)
Watson JA,Bhattacharyya BJ,Vaden JH,Wilson JA,Icyuz M,Howard AD,Phillips E,DeSilva TM,Siegal GP,Bean AJ,King GD,Phillips SE,Miller RJ,Wilson SM

Deficient development and maintenance of postsynaptic specializations in mutant mice lacking an 'adult' acetylcholine receptor subunit.
Development (Cambridge, England)124(24)5075-86(1997 Dec)
Missias AC,Mudd J,Cunningham JM,Steinbach JH,Merlie JP,Sanes JR

Acetylcholine receptor epsilon-subunit deletion causes muscle weakness and atrophy in juvenile and adult mice.
Proceedings of the National Academy of Sciences of the United States of America93(23)13286-91(1996 Nov)
Witzemann V,Schwarz H,Koenen M,Berberich C,Villarroel A,Wernig A,Brenner HR,Sakmann B

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Chrne cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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