Chrna6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrna6
Entrez Gene ID	11440
Full Name	cholinergic receptor, nicotinic, alpha polypeptide 6
Synonyms	Acra6,Nica6
General protein information	Preferred Names cholinergic receptor, nicotinic, alpha polypeptide 6 Names neuronal acetylcholine receptor subunit alpha-6 acetylcholine receptor alpha 6 neural alpha6 nAChR nicotinic acetylcholine receptor subunit alpha 6
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_021369.2	NP_067344.2	neuronal acetylcholine receptor subunit alpha-6 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHRNA6	NP_001029266.1
Drosophila melanogaster (fruit fly)	nAcRalpha-7E	NP_525079.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000329	XP_310786.3
Homo sapiens (human)	CHRNA6	NP_004189.1
Bos taurus (cattle)	CHRNA6	XP_002698774.2
Gallus gallus (chicken)	CHRNA6	NP_990695.1
	CHRNA6	XP_001099152.1
Rattus norvegicus (Norway rat)	Chrna6	NP_476532.1
Caenorhabditis elegans (roundworm)	unc-38	NP_491472.1
Xenopus tropicalis (tropical clawed frog)	LOC100495287	XP_002942944.2
Canis lupus familiaris (dog)	CHRNA6	XP_539951.2
Mus musculus (house mouse)	Chrna6	NP_067344.2
Danio rerio (zebrafish)	chrna6	NP_001036149.1

Gene Ontology
Bibliography

Related articles in PubMed

?6?2 nicotinic acetylcholine receptors influence locomotor activity and ethanol consumption.
Kamens HM, Peck C, Garrity C, Gechlik A, Jenkins BC, Rajan A
Alcohol (Fayetteville, N.Y.)6143-49(2017 Jun)

Different Hypothalamic Nicotinic ?7 Receptor Expression and Response to Low Nicotine Dose in Alcohol-Preferring and Alcohol-Avoiding Rats.
Nuutinen S, Panula P, Salminen O
Alcoholism, clinical and experimental research40(2)329-34(2016 Feb)

?6-Containing nicotinic acetylcholine receptors in midbrain dopamine neurons are poised to govern dopamine-mediated behaviors and synaptic plasticity.
Berry JN, Engle SE, McIntosh JM, Drenan RM
Neuroscience304161-75(2015 Sep)

Evidence for a role for ?6(?) nAChRs in l-dopa-induced dyskinesias using Parkinsonian ?6(?) nAChR gain-of-function mice.
Bordia T, McGregor M, McIntosh JM, Drenan RM, Quik M
Neuroscience295187-97(2015 Jun)

The nicotinic ?6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors.
Wieskopf JS, Mathur J, Limapichat W, Post MR, Al-Qazzaz M, Sorge RE, Martin LJ, Zaykin DV, Smith SB, Freitas K, Austin JS, Dai F, Zhang J, Marcovitz J, Tuttle AH, Slepian PM, Clarke S, Drenan RM, Janes J, Al Sharari S, Segall SK, Aasvang EK, Lai W, Bittner R, Richards CI, Slade GD, Kehlet H, Walker J, Maskos U, Changeux JP, Devor M, Maixner W, Diatchenko L, Belfer I, Dougherty DA, Su AI, Lummis SC, Imad Damaj M, Lester HA, Patapoutian A, Mogil JS
Science translational medicine7(287)287ra72(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using behavioral and cellular measures of alpha6 * nAChR function, study demonstrated that the ventral tegmental area is a key site of action for nicotine at alpha6 * nAChRs
Title: ?6-Containing nicotinic acetylcholine receptors in midbrain dopamine neurons are poised to govern dopamine-mediated behaviors and synaptic plasticity.

The present data show that alpha6beta2( *) nAChRs are key regulators of LIDs, and may be useful therapeutic targets for their management in Parkinson's disease.
Title: Evidence for a role for ?6(?) nAChRs in l-dopa-induced dyskinesias using Parkinsonian ?6(?) nAChR gain-of-function mice.

enhanced alpha6beta2* nAChR activity in nucleus accumbens can stimulate dopamine production
Title: Enhanced synthesis and release of dopamine in transgenic mice with gain-of-function ?6* nAChRs.

these data indicate that a6)nAChR do not have an impact on motor output.
Title: A role for ?4(non-?6)* nicotinic acetylcholine receptors in motor behavior.

Chrna6 expression decreases with death of RGCs in glaucomatous DBA/2J mice and after optic nerve crush injury, further supporting Chrna6 as a reliable RGC marker.
Title: Effects of glaucoma on Chrna6 expression in the retina.

The following Chrna6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrna6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12256	NM_021369.2 Latest version!	Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 6 (Chrna6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$286.30 $409.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrna6 ( NM_021369.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067344.2 Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 6 (Chrna6), mRNA.

Price & Availability↑

CloneID	OMu12256
Clone ID Related Accession (Same CDS sequence)	NM_021369.2
Accession Version	NM_021369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	neuronal acetylcholine receptor subunit alpha-6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC031985.1. On Jun 9, 2003 this sequence version replaced NM_021369.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031985.1, AK149262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAACA GCCGGGACCA GGGAAACCTG CACTCCGGTT TATGTCTGTG GCTATGTGGA 
TTCCTGGCTC TCTTTAAAGG CAGTACAGGC TGTGAATCTG AAGAGCAGCT CTTCCACAGG 
CTGTTTGCTC ACTACAACCG CTTCATCCGG CCGGTGGAGA ATGTCTCCGA TCCCGTCACG 
GTGCATTTTG AATTGGCAAT CACGCAACTG GCCAATGTGG ATGAAGTCAA CCAGATTATG 
GAGACCAATC TGTGGCTGCG TCACATCTGG AAGGACTACA GATTGCGTTG GGATCCAACG 
GAGTATGATG GCATCGAGAC ACTTCGAGTT CCAGCAGACA ACATCTGGAA GCCTGACATC 
GTTCTGTATA ACAATGCTGT CGGCGACTTC CAGGTCGAAG GCAAGACCAA AGCTCTTCTC 
AAGTATGACG GTGTGATAAC CTGGACCCCA CCAGCCATCT TTAAGAGCTC CTGCCCAATG 
GACATCACCT TCTTCCCGTT TGACCATCAA AACTGCTCCC TGAAGTTTGG TTCCTGGACT 
TATGACAAGG CAGAAATCGA CCTTCTCATC ATTGGCTCTA AAGTAGACAT GAACGACTTT 
TGGGAAAACA GTGAATGGGA AATTGTCGAC GCCTCTGGCT ATAAGCATGA CATCAAGTAC 
AACTGCTGTG AAGAGATTTA CACGGACATA ACCTACTCCT TCTACATCAG GAGGTTGCCC 
ATGTTTTACA CCATCAACCT CATCATCCCC TGCCTCTTCA TTTCCTTCCT CACGGTGCTG 
GTTTTTTACC TTCCCTCCGA CTGTGGCGAG AAAGTGACTC TTTGCATCTC CGTTCTGCTT 
TCTCTCACTG TCTTTTTGCT GGTGATTACA GAGACCATCC CATCCACATC TCTCGTGATC 
CCACTGGTGG GTGAGTATCT ACTGTTCACC ATGATCTTTG TCACGCTGTC CATTGTGGTG 
ACCGTGTTCG TGCTGAACAT ACACTACAGG ACCCCAGCAA CGCATACCAT GCCCAAGTGG 
GTGAAGACCA TCTTCCTTCA GGCCTTCCCC TCGATTCTGA TGATGAGGAA ACCTCTGGAC 
AAGACAAAGG AGGCAGGAGG TGTTAAGGAC CCCAAAAGCC ATACCAAGAG GCCTGCCAAG 
GTCAAATTTA CTCATCGAGG AGAATCCAAA CTTCTAAAGG AATGCCACCA CTGCCAAAAA 
TCAAGTGACA TAGCACCTGG AAAGAGAAGA TCAAGCCAGC AGCCTGCACG GTGGGTGGCA 
GAGAATTCAG AGCACTCGTC CGATGTTGAA GATGTCATCG AGAGTGTTCA ATTCATAGCA 
GAAAACATGA AGAGCCACAA TGAAACAAAC GAGGTAGAAG ACGACTGGAA ATACATGGCT 
ATGGTGGTGG ACAGAGTCTT CCTTTGGGTA TTTATAATTG TCTGTGTGTT TGGAACTGTG 
GGGCTATTTC TGCAGCCACT GCTTGGGAAC ACAGGAAAGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067344.2
CDS	243..1727
Translation	MLNSRDQGNLHSGLCLWLCGFLALFKGSTGCESEEQLFHRLFAHYNRFIRPVENVSDPVTVHFELAITQLANVDEVNQIMETNLWLRHIWKDYRLRWDPTEYDGIETLRVPADNIWKPDIVLYNNAVGDFQVEGKTKALLKYDGVITWTPPAIFKSSCPMDITFFPFDHQNCSLKFGSWTYDKAEIDLLIIGSKVDMNDFWENSEWEIVDASGYKHDIKYNCCEEIYTDITYSFYIRRLPMFYTINLIIPCLFISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLVGEYLLFTMIFVTLSIVVTVFVLNIHYRTPATHTMPKWVKTIFLQAFPSILMMRKPLDKTKEAGGVKDPKSHTKRPAKVKFTHRGESKLLKECHHCQKSSDIAPGKRRSSQQPARWVAENSEHSSDVEDVIESVQFIAENMKSHNETNEVEDDWKYMAMVVDRVFLWVFIIVCVFGTVGLFLQPLLGNTGKS

Target ORF information:

RefSeq Version	NM_021369.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 6 (Chrna6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021369.2

ORF Insert Sequence:

ATGCTGAACA GCCGGGACCA GGGAAACCTG CACTCCGGTT TATGTCTGTG GCTATGTGGA 
TTCCTGGCTC TCTTTAAAGG CAGTACAGGC TGTGAATCTG AAGAGCAGCT CTTCCACAGG 
CTGTTTGCTC ACTACAACCG CTTCATCCGG CCGGTGGAGA ATGTCTCCGA TCCCGTCACG 
GTGCATTTTG AATTGGCAAT CACGCAACTG GCCAATGTGG ATGAAGTCAA CCAGATTATG 
GAGACCAATC TGTGGCTGCG TCACATCTGG AAGGACTACA GATTGCGTTG GGATCCAACG 
GAGTATGATG GCATCGAGAC ACTTCGAGTT CCAGCAGACA ACATCTGGAA GCCTGACATC 
GTTCTGTATA ACAATGCTGT CGGCGACTTC CAGGTCGAAG GCAAGACCAA AGCTCTTCTC 
AAGTATGACG GTGTGATAAC CTGGACCCCA CCAGCCATCT TTAAGAGCTC CTGCCCAATG 
GACATCACCT TCTTCCCGTT TGACCATCAA AACTGCTCCC TGAAGTTTGG TTCCTGGACT 
TATGACAAGG CAGAAATCGA CCTTCTCATC ATTGGCTCTA AAGTAGACAT GAACGACTTT 
TGGGAAAACA GTGAATGGGA AATTGTCGAC GCCTCTGGCT ATAAGCATGA CATCAAGTAC 
AACTGCTGTG AAGAGATTTA CACGGACATA ACCTACTCCT TCTACATCAG GAGGTTGCCC 
ATGTTTTACA CCATCAACCT CATCATCCCC TGCCTCTTCA TTTCCTTCCT CACGGTGCTG 
GTTTTTTACC TTCCCTCCGA CTGTGGCGAG AAAGTGACTC TTTGCATCTC CGTTCTGCTT 
TCTCTCACTG TCTTTTTGCT GGTGATTACA GAGACCATCC CATCCACATC TCTCGTGATC 
CCACTGGTGG GTGAGTATCT ACTGTTCACC ATGATCTTTG TCACGCTGTC CATTGTGGTG 
ACCGTGTTCG TGCTGAACAT ACACTACAGG ACCCCAGCAA CGCATACCAT GCCCAAGTGG 
GTGAAGACCA TCTTCCTTCA GGCCTTCCCC TCGATTCTGA TGATGAGGAA ACCTCTGGAC 
AAGACAAAGG AGGCAGGAGG TGTTAAGGAC CCCAAAAGCC ATACCAAGAG GCCTGCCAAG 
GTCAAATTTA CTCATCGAGG AGAATCCAAA CTTCTAAAGG AATGCCACCA CTGCCAAAAA 
TCAAGTGACA TAGCACCTGG AAAGAGAAGA TCAAGCCAGC AGCCTGCACG GTGGGTGGCA 
GAGAATTCAG AGCACTCGTC CGATGTTGAA GATGTCATCG AGAGTGTTCA ATTCATAGCA 
GAAAACATGA AGAGCCACAA TGAAACAAAC GAGGTAGAAG ACGACTGGAA ATACATGGCT 
ATGGTGGTGG ACAGAGTCTT CCTTTGGGTA TTTATAATTG TCTGTGTGTT TGGAACTGTG 
GGGCTATTTC TGCAGCCACT GCTTGGGAAC ACAGGAAAGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?6?2 nicotinic acetylcholine receptors influence locomotor activity and ethanol consumption.
Alcohol (Fayetteville, N.Y.)6143-49(2017 Jun)
Kamens HM,Peck C,Garrity C,Gechlik A,Jenkins BC,Rajan A

Different Hypothalamic Nicotinic ?7 Receptor Expression and Response to Low Nicotine Dose in Alcohol-Preferring and Alcohol-Avoiding Rats.
Alcoholism, clinical and experimental research40(2)329-34(2016 Feb)
Nuutinen S,Panula P,Salminen O

?6-Containing nicotinic acetylcholine receptors in midbrain dopamine neurons are poised to govern dopamine-mediated behaviors and synaptic plasticity.
Neuroscience304161-75(2015 Sep)
Berry JN,Engle SE,McIntosh JM,Drenan RM

Evidence for a role for ?6(?) nAChRs in l-dopa-induced dyskinesias using Parkinsonian ?6(?) nAChR gain-of-function mice.
Neuroscience295187-97(2015 Jun)
Bordia T,McGregor M,McIntosh JM,Drenan RM,Quik M

The nicotinic ?6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors.
Science translational medicine7(287)287ra72(2015 May)
Wieskopf JS,Mathur J,Limapichat W,Post MR,Al-Qazzaz M,Sorge RE,Martin LJ,Zaykin DV,Smith SB,Freitas K,Austin JS,Dai F,Zhang J,Marcovitz J,Tuttle AH,Slepian PM,Clarke S,Drenan RM,Janes J,Al Sharari S,Segall SK,Aasvang EK,Lai W,Bittner R,Richards CI,Slade GD,Kehlet H,Walker J,Maskos U,Changeux JP,Devor M,Maixner W,Diatchenko L,Belfer I,Dougherty DA,Su AI,Lummis SC,Imad Damaj M,Lester HA,Patapoutian A,Mogil JS

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Chrna6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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