Acp2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acp2
Entrez Gene ID	11432
Full Name	acid phosphatase 2, lysosomal
Synonyms	Acp-2,LAP
General protein information	Preferred Names acid phosphatase 2, lysosomal Names lysosomal acid phosphatase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 E1\|2 50.54 cM
Summary	The protein encoded by this gene belongs to the histidine acid phosphatase family, which hydrolyze orthophosphoric monoesters to alcohol and phosphate. This protein is localized to the lysosomal membrane, and is chemically and genetically distinct from the red cell acid phosphatase. Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures. An enzymatically-inactive allele of this gene showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001357067.1	NP_001343996.1	lysosomal acid phosphatase isoform 1x precursor
XM_011239257.2	XP_011237559.1	lysosomal acid phosphatase isoform X1
XM_006498585.3	XP_006498648.1	lysosomal acid phosphatase isoform X2
XM_011239257.3	XP_011237559.1	lysosomal acid phosphatase isoform X1
NM_007387.2	NP_031413.1	lysosomal acid phosphatase isoform 1 precursor

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002387	XP_312568.5
Xenopus tropicalis (tropical clawed frog)	acp2	NP_001072207.1
Gallus gallus (chicken)	ACP2	NP_001026719.1
Drosophila melanogaster (fruit fly)	Acph-1	NP_524917.1
	ACP2	XP_001109604.1
Mus musculus (house mouse)	Acp2	NP_031413.1
Bos taurus (cattle)	ACP2	NP_001069526.1
Danio rerio (zebrafish)	acp2	NP_001013355.1
Caenorhabditis elegans (roundworm)	pho-5	NP_498604.2
Homo sapiens (human)	ACP2	NP_001601.1
Pan troglodytes (chimpanzee)	ACP2	NP_001233418.1
Canis lupus familiaris (dog)	ACP2	XP_850233.1
Rattus norvegicus (Norway rat)	Acp2	NP_058684.2

Gene Ontology
Bibliography

Related articles in PubMed

Evidence of efficient stop codon readthrough in four mammalian genes.
Loughran G, Chou MY, Ivanov IP, Jungreis I, Kellis M, Kiran AM, Baranov PV, Atkins JF
Nucleic acids research42(14)8928-38(2014 Aug)

Purkinje cell compartmentation in the cerebellum of the lysosomal Acid phosphatase 2 mutant mouse (nax - naked-ataxia mutant mouse).
Bailey K, Rahimi Balaei M, Mannan A, Del Bigio MR, Marzban H
PloS one9(4)e94327(2014)

Spatial and temporal expression of lysosomal acid phosphatase 2 (ACP2) reveals dynamic patterning of the mouse cerebellar cortex.
Bailey K, Rahimi Balaei M, Mehdizadeh M, Marzban H
Cerebellum (London, England)12(6)870-81(2013 Dec)

Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5.
Makrypidi G, Damme M, M?ller-Loennies S, Trusch M, Schmidt B, Schl?ter H, Heeren J, L?bke T, Saftig P, Braulke T
Molecular and cellular biology32(4)774-82(2012 Feb)

Increased expression of lysosomal acid phosphatase in CLN3-defective cells and mouse brain tissue.
Pohl S, Mitchison HM, Kohlsch?tter A, van Diggelen O, Braulke T, Storch S
Journal of neurochemistry103(6)2177-88(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ACP2 has critical roles in the development of the anterior cerebellum and it may regulate anterior and central zone compartmentation.
Title: Purkinje cell compartmentation in the cerebellum of the lysosomal Acid phosphatase 2 mutant mouse (nax - naked-ataxia mutant mouse).

These data suggest that ACP2 expression is dynamically regulated during development, and in the adult, it may function within a complex architecture that is linked to cerebellar modular organization.
Title: Spatial and temporal expression of lysosomal acid phosphatase 2 (ACP2) reveals dynamic patterning of the mouse cerebellar cortex.

dephosphorylation of Man6P-containing lysosomal proteins requires the concerted action of Acp2 and Acp5 and is needed for hydrolysis and removal of degradation products
Title: Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5.

The data suggest that lysosomal dysfunction and accumulation of storage material require increased biogenesis of LAP/ACP2 and LAMP-2 positive membranes which makes LAP/ACP2 suitable as biomarker of Batten disease.
Title: Increased expression of lysosomal acid phosphatase in CLN3-defective cells and mouse brain tissue.

Acp2 is the gene mutated in nax mice provides a valuable model system for studying the role of Acp2 in cerebellum and skin homeostasis.
Title: Mutation in the gene encoding lysosomal acid phosphatase (Acp2) causes cerebellum and skin malformation in mouse.

The following Acp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83821	NM_001357067.1 Latest version!	Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu29925	XM_006498585.3 Latest version!	Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu00087	NM_007387.2 Latest version!	Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu67053	XM_011239257.3 Latest version!	Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu67053	XM_011239257.2	Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acp2 ( NM_001357067.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343996.1 Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu83821
Clone ID Related Accession (Same CDS sequence)	NM_001357067.1
Accession Version	NM_001357067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	lysosomal acid phosphatase isoform 1x precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691450.15, AK133756.1, AK128963.1, BQ445129.1, CD741498.1 and AW061158.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer isoform (1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. ##Evidence-Data-START## Transcript exon combination :: AK170658.1, AK128963.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGCA GACAGACTGG TTGGAGCCAG GCGGCTCTTC TCCAGTTCCT TCTTGGCATG 
TGCCTAACGG TGATGCCACC CATACAGGCC CGGAGTCTAC GCTTTGTTAC CTTGCTGTAT 
CGACATGGAG ATCGGTCACC AGTGAAGACA TATCCCAAGG ACCCCTATCA GGAAGAGAAA 
TGGCCCCAGG GATTTGGTCA GCTAACCAAG GAAGGGATGC TACAGCACTG GGAGCTGGGC 
CAGGCCCTGC GGCAACGCTA CCATGGCTTT CTGAACACCT CTTACCACAG GCAAGAGGTT 
TATGTGCGAA GCACAGACTT CGATCGTACT CTCATGAGTG CTGAGGCCAA CCTGGCTGGA 
CTCTTCCCTC CCAATGAAGT TCAGCACTTC AACCCTAACA TTTCATGGCA GCCTATCCCT 
GTTCACACTG TGCCCATCAC TGAAGACAGG TTGCTGAAGT TTCCTTTGGG TCCATGTCCC 
CGTTATGAGC AGCTGCAGAA TGAGACTCGG CAGACACCAG AGTATCAGAA CAGAAGTATT 
CAGAATGCAC AATTTCTGAA CATGGTGGCC AACGAGACAG GGCTTACAAA CGTGACCCTA 
GAGACCATCT GGAATGTGTA TGACACACTC TTTTGTGAGC AAACCCATGG GCTGCTTCTG 
CCGCCCTGGG CCTCACCCCA AACCGTGCAG CGTCTGAGCC AGCTAAAGGA CTTCAGCTTC 
CTCTTCCTCT TCGGGATCCA CGAGCAAGTA CAGAAGGCCC GGCTTCAGGG GGGAGTTCTG 
CTGGCTCAGA TATTGAAGAA CCTGACCCTA ATGGCAACTA CCTCTCAATT CCCTAAGCTT 
CTGGTTTATT CCGCGCATGA CACTACCCTG GTTGCTCTGC AAATGGCATT GAATGTCTAC 
AATGGGAAGC AAGCTCCCTA TGCTTCCTGC CACATATTTG AACTGTACCA GGAAGATAAT 
GGGAATTTCT CAGTCGAGAT GTACTTTCGG AATGACAGTA AGAAGGCACC CTGGCCTCTG 
ATCCTGCCTG GCTGTCCTCA CCGTTGCCCA CTGCAGGATT TCCTTCGCCT CACAGAGCCT 
GTCATACCCA AGGACTGGCA GAAGGAGTGC CAGCTAGCAA ATGATACTGC AGACACAGAG 
GTAATTGTGG CATTGGCTGT CTGTGGCTCC ATCCTCTTCC TTCTCATAGT GTTGCTCCTC 
ACCATCCTCT TCCGGATGCA GGCCCAGCCT CCTGGCTACC ACCATGTTGC AGACAGGGAA 
GACCATGCTT GACAACCACT CAGTCCCCTT CCCTCTGCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343996.1
CDS	24..1328
Translation	MAGRQTGWSQAALLQFLLGMCLTVMPPIQARSLRFVTLLYRHGDRSPVKTYPKDPYQEEKWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNEVQHFNPNISWQPIPVHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNRSIQNAQFLNMVANETGLTNVTLETIWNVYDTLFCEQTHGLLLPPWASPQTVQRLSQLKDFSFLFLFGIHEQVQKARLQGGVLLAQILKNLTLMATTSQFPKLLVYSAHDTTLVALQMALNVYNGKQAPYASCHIFELYQEDNGNFSVEMYFRNDSKKAPWPLILPGCPHRCPLQDFLRLTEPVIPKDWQKECQLANDTADTEVIVALAVCGSILFLLIVLLLTILFRMQAQPPGYHHVADREDHA*QPLSPLPSAS

Target ORF information:

RefSeq Version	NM_001357067.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357067.1

ORF Insert Sequence:

ATGGCCGGCA GACAGACTGG TTGGAGCCAG GCGGCTCTTC TCCAGTTCCT TCTTGGCATG 
TGCCTAACGG TGATGCCACC CATACAGGCC CGGAGTCTAC GCTTTGTTAC CTTGCTGTAT 
CGACATGGAG ATCGGTCACC AGTGAAGACA TATCCCAAGG ACCCCTATCA GGAAGAGAAA 
TGGCCCCAGG GATTTGGTCA GCTAACCAAG GAAGGGATGC TACAGCACTG GGAGCTGGGC 
CAGGCCCTGC GGCAACGCTA CCATGGCTTT CTGAACACCT CTTACCACAG GCAAGAGGTT 
TATGTGCGAA GCACAGACTT CGATCGTACT CTCATGAGTG CTGAGGCCAA CCTGGCTGGA 
CTCTTCCCTC CCAATGAAGT TCAGCACTTC AACCCTAACA TTTCATGGCA GCCTATCCCT 
GTTCACACTG TGCCCATCAC TGAAGACAGG TTGCTGAAGT TTCCTTTGGG TCCATGTCCC 
CGTTATGAGC AGCTGCAGAA TGAGACTCGG CAGACACCAG AGTATCAGAA CAGAAGTATT 
CAGAATGCAC AATTTCTGAA CATGGTGGCC AACGAGACAG GGCTTACAAA CGTGACCCTA 
GAGACCATCT GGAATGTGTA TGACACACTC TTTTGTGAGC AAACCCATGG GCTGCTTCTG 
CCGCCCTGGG CCTCACCCCA AACCGTGCAG CGTCTGAGCC AGCTAAAGGA CTTCAGCTTC 
CTCTTCCTCT TCGGGATCCA CGAGCAAGTA CAGAAGGCCC GGCTTCAGGG GGGAGTTCTG 
CTGGCTCAGA TATTGAAGAA CCTGACCCTA ATGGCAACTA CCTCTCAATT CCCTAAGCTT 
CTGGTTTATT CCGCGCATGA CACTACCCTG GTTGCTCTGC AAATGGCATT GAATGTCTAC 
AATGGGAAGC AAGCTCCCTA TGCTTCCTGC CACATATTTG AACTGTACCA GGAAGATAAT 
GGGAATTTCT CAGTCGAGAT GTACTTTCGG AATGACAGTA AGAAGGCACC CTGGCCTCTG 
ATCCTGCCTG GCTGTCCTCA CCGTTGCCCA CTGCAGGATT TCCTTCGCCT CACAGAGCCT 
GTCATACCCA AGGACTGGCA GAAGGAGTGC CAGCTAGCAA ATGATACTGC AGACACAGAG 
GTAATTGTGG CATTGGCTGT CTGTGGCTCC ATCCTCTTCC TTCTCATAGT GTTGCTCCTC 
ACCATCCTCT TCCGGATGCA GGCCCAGCCT CCTGGCTACC ACCATGTTGC AGACAGGGAA 
GACCATGCTT GACAACCACT CAGTCCCCTT CCCTCTGCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acp2 ( XM_006498585.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498648.1 Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu29925
Clone ID Related Accession (Same CDS sequence)	XM_006498585.3
Accession Version	XM_006498585.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysosomal acid phosphatase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGCA GACAGACTGG TTGGAGCCAG GCGGCTCTTC TCCAGTTCCT TCTTGGCATG 
TGCCTAACGG TGATGCCACC CATACAGGCC CGGAGTCTAC GCTTTGTTAC CTTGCTGTAT 
CGACATGGAG ATCGGTCACC AGTGAAGACA TATCCCAAGG ACCCCTATCA GGAAGAGAAA 
TGGCCCCAGG GATTTGGTCA GCTAACCAAG GAAGGGATGC TACAGCACTG GGAGCTGGGC 
CAGGCCCTGC GGCAACGCTA CCATGGCTTT CTGAACACCT CTTACCACAG GCAAGAGGTT 
TATGTGCGAA GCACAGACTT CGATCGTACT CTCATGAGTG CTGAGGCCAA CCTGGCTGGA 
CTCTTCCCTC CCAATGAAGT TCAGCACTTC AACCCTAACA TTTCATGGCA GCCTATCCCT 
GTTCACACTG TGCCCATCAC TGAAGACAGG TTGCTGAAGT TTCCTTTGGG TCCATGTCCC 
CGTTATGAGC AGCTGCAGAA TGAGACTCGG CAGACACCAG AGTATCAGAA CAGAAGTATT 
CAGAATGCAC AATTTCTGAA CATGGTGGCC AACGAGACAG GGCTTACAAA CGTGACCCTA 
GAGACCATCT GGAATGTGTA TGACACACTC TTTTGTGAGG CACCTTCCCC ACCTGGGGAA 
GAAAGCCACC CTTGGAGAGG CTCTGGCCTA GGCCTAGGGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498648.1
CDS	11..715
Translation	MAGRQTGWSQAALLQFLLGMCLTVMPPIQARSLRFVTLLYRHGDRSPVKTYPKDPYQEEKWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNEVQHFNPNISWQPIPVHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNRSIQNAQFLNMVANETGLTNVTLETIWNVYDTLFCEAPSPPGEESHPWRGSGLGLGS

Target ORF information:

RefSeq Version	XM_006498585.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498585.3

ORF Insert Sequence:

ATGGCCGGCA GACAGACTGG TTGGAGCCAG GCGGCTCTTC TCCAGTTCCT TCTTGGCATG 
TGCCTAACGG TGATGCCACC CATACAGGCC CGGAGTCTAC GCTTTGTTAC CTTGCTGTAT 
CGACATGGAG ATCGGTCACC AGTGAAGACA TATCCCAAGG ACCCCTATCA GGAAGAGAAA 
TGGCCCCAGG GATTTGGTCA GCTAACCAAG GAAGGGATGC TACAGCACTG GGAGCTGGGC 
CAGGCCCTGC GGCAACGCTA CCATGGCTTT CTGAACACCT CTTACCACAG GCAAGAGGTT 
TATGTGCGAA GCACAGACTT CGATCGTACT CTCATGAGTG CTGAGGCCAA CCTGGCTGGA 
CTCTTCCCTC CCAATGAAGT TCAGCACTTC AACCCTAACA TTTCATGGCA GCCTATCCCT 
GTTCACACTG TGCCCATCAC TGAAGACAGG TTGCTGAAGT TTCCTTTGGG TCCATGTCCC 
CGTTATGAGC AGCTGCAGAA TGAGACTCGG CAGACACCAG AGTATCAGAA CAGAAGTATT 
CAGAATGCAC AATTTCTGAA CATGGTGGCC AACGAGACAG GGCTTACAAA CGTGACCCTA 
GAGACCATCT GGAATGTGTA TGACACACTC TTTTGTGAGG CACCTTCCCC ACCTGGGGAA 
GAAAGCCACC CTTGGAGAGG CTCTGGCCTA GGCCTAGGGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acp2 ( XM_011239257.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237559.1 Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67053
Clone ID Related Accession (Same CDS sequence)	XM_011239257.2 , XM_011239257.3
Accession Version	XM_011239257.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysosomal acid phosphatase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGG CTGCACTTCC GGCAAGTGTC CTTACGAATG CATCCGGGCG GCTGCAGCAG 
CTGTATCGAC ATGGAGATCG GTCACCAGTG AAGACATATC CCAAGGACCC CTATCAGGAA 
GAGAAATGGC CCCAGGGATT TGGTCAGCTA ACCAAGGAAG GGATGCTACA GCACTGGGAG 
CTGGGCCAGG CCCTGCGGCA ACGCTACCAT GGCTTTCTGA ACACCTCTTA CCACAGGCAA 
GAGGTTTATG TGCGAAGCAC AGACTTCGAT CGTACTCTCA TGAGTGCTGA GGCCAACCTG 
GCTGGACTCT TCCCTCCCAA TGAAGTTCAG CACTTCAACC CTAACATTTC ATGGCAGCCT 
ATCCCTGTTC ACACTGTGCC CATCACTGAA GACAGGTTGC TGAAGTTTCC TTTGGGTCCA 
TGTCCCCGTT ATGAGCAGCT GCAGAATGAG ACTCGGCAGA CACCAGAGTA TCAGAACAGA 
AGTATTCAGA ATGCACAATT TCTGAACATG GTGGCCAACG AGACAGGGCT TACAAACGTG 
ACCCTAGAGA CCATCTGGAA TGTGTATGAC ACACTCTTTT GTGAGCAAAC CCATGGGCTG 
CTTCTGCCGC CCTGGGCCTC ACCCCAAACC GTGCAGCGTC TGAGCCAGCT AAAGGACTTC 
AGCTTCCTCT TCCTCTTCGG GATCCACGAG CAAGTACAGA AGGCCCGGCT TCAGGGGGGA 
GTTCTGCTGG CTCAGATATT GAAGAACCTG ACCCTAATGG CAACTACCTC TCAATTCCCT 
AAGCTTCTGG TTTATTCCGC GCATGACACT ACCCTGGTTG CTCTGCAAAT GGCATTGAAT 
GTCTACAATG GGAAGCAAGC TCCCTATGCT TCCTGCCACA TATTTGAACT GTACCAGGAA 
GATAATGGGA ATTTCTCAGT CGAGATGTAC TTTCGGAATG ACAGTAAGAA GGCACCCTGG 
CCTCTGATCC TGCCTGGCTG TCCTCACCGT TGCCCACTGC AGGATTTCCT TCGCCTCACA 
GAGCCTGTCA TACCCAAGGA CTGGCAGAAG GAGTGCCAGC TAGCAAATGA TACTGCAGAC 
ACAGAGGTAA TTGTGGCATT GGCTGTCTGT GGCTCCATCC TCTTCCTTCT CATAGTGTTG 
CTCCTCACCA TCCTCTTCCG GATGCAGGCC CAGCCTCCTG GCTACCACCA TGTTGCAGAC 
AGGGAAGACC ATGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237559.1
CDS	16..1233
Translation	MAKAALPASVLTNASGRLQQLYRHGDRSPVKTYPKDPYQEEKWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNEVQHFNPNISWQPIPVHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNRSIQNAQFLNMVANETGLTNVTLETIWNVYDTLFCEQTHGLLLPPWASPQTVQRLSQLKDFSFLFLFGIHEQVQKARLQGGVLLAQILKNLTLMATTSQFPKLLVYSAHDTTLVALQMALNVYNGKQAPYASCHIFELYQEDNGNFSVEMYFRNDSKKAPWPLILPGCPHRCPLQDFLRLTEPVIPKDWQKECQLANDTADTEVIVALAVCGSILFLLIVLLLTILFRMQAQPPGYHHVADREDHA

Target ORF information:

RefSeq Version	XM_011239257.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239257.2

ORF Insert Sequence:

ATGGCGAAGG CTGCACTTCC GGCAAGTGTC CTTACGAATG CATCCGGGCG GCTGCAGCAG 
CTGTATCGAC ATGGAGATCG GTCACCAGTG AAGACATATC CCAAGGACCC CTATCAGGAA 
GAGAAATGGC CCCAGGGATT TGGTCAGCTA ACCAAGGAAG GGATGCTACA GCACTGGGAG 
CTGGGCCAGG CCCTGCGGCA ACGCTACCAT GGCTTTCTGA ACACCTCTTA CCACAGGCAA 
GAGGTTTATG TGCGAAGCAC AGACTTCGAT CGTACTCTCA TGAGTGCTGA GGCCAACCTG 
GCTGGACTCT TCCCTCCCAA TGAAGTTCAG CACTTCAACC CTAACATTTC ATGGCAGCCT 
ATCCCTGTTC ACACTGTGCC CATCACTGAA GACAGGTTGC TGAAGTTTCC TTTGGGTCCA 
TGTCCCCGTT ATGAGCAGCT GCAGAATGAG ACTCGGCAGA CACCAGAGTA TCAGAACAGA 
AGTATTCAGA ATGCACAATT TCTGAACATG GTGGCCAACG AGACAGGGCT TACAAACGTG 
ACCCTAGAGA CCATCTGGAA TGTGTATGAC ACACTCTTTT GTGAGCAAAC CCATGGGCTG 
CTTCTGCCGC CCTGGGCCTC ACCCCAAACC GTGCAGCGTC TGAGCCAGCT AAAGGACTTC 
AGCTTCCTCT TCCTCTTCGG GATCCACGAG CAAGTACAGA AGGCCCGGCT TCAGGGGGGA 
GTTCTGCTGG CTCAGATATT GAAGAACCTG ACCCTAATGG CAACTACCTC TCAATTCCCT 
AAGCTTCTGG TTTATTCCGC GCATGACACT ACCCTGGTTG CTCTGCAAAT GGCATTGAAT 
GTCTACAATG GGAAGCAAGC TCCCTATGCT TCCTGCCACA TATTTGAACT GTACCAGGAA 
GATAATGGGA ATTTCTCAGT CGAGATGTAC TTTCGGAATG ACAGTAAGAA GGCACCCTGG 
CCTCTGATCC TGCCTGGCTG TCCTCACCGT TGCCCACTGC AGGATTTCCT TCGCCTCACA 
GAGCCTGTCA TACCCAAGGA CTGGCAGAAG GAGTGCCAGC TAGCAAATGA TACTGCAGAC 
ACAGAGGTAA TTGTGGCATT GGCTGTCTGT GGCTCCATCC TCTTCCTTCT CATAGTGTTG 
CTCCTCACCA TCCTCTTCCG GATGCAGGCC CAGCCTCCTG GCTACCACCA TGTTGCAGAC 
AGGGAAGACC ATGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acp2 ( NM_007387.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031413.1 Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant 1, mRNA.

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CloneID	OMu00087
Clone ID Related Accession (Same CDS sequence)	NM_007387.2
Accession Version	NM_007387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	lysosomal acid phosphatase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691450.15, AK133756.1, AK128963.1, BQ445129.1, CD741498.1 and AW061158.1. On Apr 25, 2010 this sequence version replaced NM_007387.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK170658.1, AK128963.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGCA GACAGACTGG TTGGAGCCAG GCGGCTCTTC TCCAGTTCCT TCTTGGCATG 
TGCCTAACGG TGATGCCACC CATACAGGCC CGGAGTCTAC GCTTTGTTAC CTTGCTGTAT 
CGACATGGAG ATCGGTCACC AGTGAAGACA TATCCCAAGG ACCCCTATCA GGAAGAGAAA 
TGGCCCCAGG GATTTGGTCA GCTAACCAAG GAAGGGATGC TACAGCACTG GGAGCTGGGC 
CAGGCCCTGC GGCAACGCTA CCATGGCTTT CTGAACACCT CTTACCACAG GCAAGAGGTT 
TATGTGCGAA GCACAGACTT CGATCGTACT CTCATGAGTG CTGAGGCCAA CCTGGCTGGA 
CTCTTCCCTC CCAATGAAGT TCAGCACTTC AACCCTAACA TTTCATGGCA GCCTATCCCT 
GTTCACACTG TGCCCATCAC TGAAGACAGG TTGCTGAAGT TTCCTTTGGG TCCATGTCCC 
CGTTATGAGC AGCTGCAGAA TGAGACTCGG CAGACACCAG AGTATCAGAA CAGAAGTATT 
CAGAATGCAC AATTTCTGAA CATGGTGGCC AACGAGACAG GGCTTACAAA CGTGACCCTA 
GAGACCATCT GGAATGTGTA TGACACACTC TTTTGTGAGC AAACCCATGG GCTGCTTCTG 
CCGCCCTGGG CCTCACCCCA AACCGTGCAG CGTCTGAGCC AGCTAAAGGA CTTCAGCTTC 
CTCTTCCTCT TCGGGATCCA CGAGCAAGTA CAGAAGGCCC GGCTTCAGGG GGGAGTTCTG 
CTGGCTCAGA TATTGAAGAA CCTGACCCTA ATGGCAACTA CCTCTCAATT CCCTAAGCTT 
CTGGTTTATT CCGCGCATGA CACTACCCTG GTTGCTCTGC AAATGGCATT GAATGTCTAC 
AATGGGAAGC AAGCTCCCTA TGCTTCCTGC CACATATTTG AACTGTACCA GGAAGATAAT 
GGGAATTTCT CAGTCGAGAT GTACTTTCGG AATGACAGTA AGAAGGCACC CTGGCCTCTG 
ATCCTGCCTG GCTGTCCTCA CCGTTGCCCA CTGCAGGATT TCCTTCGCCT CACAGAGCCT 
GTCATACCCA AGGACTGGCA GAAGGAGTGC CAGCTAGCAA ATGATACTGC AGACACAGAG 
GTAATTGTGG CATTGGCTGT CTGTGGCTCC ATCCTCTTCC TTCTCATAGT GTTGCTCCTC 
ACCATCCTCT TCCGGATGCA GGCCCAGCCT CCTGGCTACC ACCATGTTGC AGACAGGGAA 
GACCATGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031413.1
CDS	24..1295
Translation	MAGRQTGWSQAALLQFLLGMCLTVMPPIQARSLRFVTLLYRHGDRSPVKTYPKDPYQEEKWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNEVQHFNPNISWQPIPVHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNRSIQNAQFLNMVANETGLTNVTLETIWNVYDTLFCEQTHGLLLPPWASPQTVQRLSQLKDFSFLFLFGIHEQVQKARLQGGVLLAQILKNLTLMATTSQFPKLLVYSAHDTTLVALQMALNVYNGKQAPYASCHIFELYQEDNGNFSVEMYFRNDSKKAPWPLILPGCPHRCPLQDFLRLTEPVIPKDWQKECQLANDTADTEVIVALAVCGSILFLLIVLLLTILFRMQAQPPGYHHVADREDHA

Target ORF information:

RefSeq Version	NM_007387.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007387.2

ORF Insert Sequence:

ATGGCCGGCA GACAGACTGG TTGGAGCCAG GCGGCTCTTC TCCAGTTCCT TCTTGGCATG 
TGCCTAACGG TGATGCCACC CATACAGGCC CGGAGTCTAC GCTTTGTTAC CTTGCTGTAT 
CGACATGGAG ATCGGTCACC AGTGAAGACA TATCCCAAGG ACCCCTATCA GGAAGAGAAA 
TGGCCCCAGG GATTTGGTCA GCTAACCAAG GAAGGGATGC TACAGCACTG GGAGCTGGGC 
CAGGCCCTGC GGCAACGCTA CCATGGCTTT CTGAACACCT CTTACCACAG GCAAGAGGTT 
TATGTGCGAA GCACAGACTT CGATCGTACT CTCATGAGTG CTGAGGCCAA CCTGGCTGGA 
CTCTTCCCTC CCAATGAAGT TCAGCACTTC AACCCTAACA TTTCATGGCA GCCTATCCCT 
GTTCACACTG TGCCCATCAC TGAAGACAGG TTGCTGAAGT TTCCTTTGGG TCCATGTCCC 
CGTTATGAGC AGCTGCAGAA TGAGACTCGG CAGACACCAG AGTATCAGAA CAGAAGTATT 
CAGAATGCAC AATTTCTGAA CATGGTGGCC AACGAGACAG GGCTTACAAA CGTGACCCTA 
GAGACCATCT GGAATGTGTA TGACACACTC TTTTGTGAGC AAACCCATGG GCTGCTTCTG 
CCGCCCTGGG CCTCACCCCA AACCGTGCAG CGTCTGAGCC AGCTAAAGGA CTTCAGCTTC 
CTCTTCCTCT TCGGGATCCA CGAGCAAGTA CAGAAGGCCC GGCTTCAGGG GGGAGTTCTG 
CTGGCTCAGA TATTGAAGAA CCTGACCCTA ATGGCAACTA CCTCTCAATT CCCTAAGCTT 
CTGGTTTATT CCGCGCATGA CACTACCCTG GTTGCTCTGC AAATGGCATT GAATGTCTAC 
AATGGGAAGC AAGCTCCCTA TGCTTCCTGC CACATATTTG AACTGTACCA GGAAGATAAT 
GGGAATTTCT CAGTCGAGAT GTACTTTCGG AATGACAGTA AGAAGGCACC CTGGCCTCTG 
ATCCTGCCTG GCTGTCCTCA CCGTTGCCCA CTGCAGGATT TCCTTCGCCT CACAGAGCCT 
GTCATACCCA AGGACTGGCA GAAGGAGTGC CAGCTAGCAA ATGATACTGC AGACACAGAG 
GTAATTGTGG CATTGGCTGT CTGTGGCTCC ATCCTCTTCC TTCTCATAGT GTTGCTCCTC 
ACCATCCTCT TCCGGATGCA GGCCCAGCCT CCTGGCTACC ACCATGTTGC AGACAGGGAA 
GACCATGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acp2 ( XM_011239257.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237559.1 Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67053
Clone ID Related Accession (Same CDS sequence)	XM_011239257.2 , XM_011239257.3
Accession Version	XM_011239257.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysosomal acid phosphatase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGG CTGCACTTCC GGCAAGTGTC CTTACGAATG CATCCGGGCG GCTGCAGCAG 
CTGTATCGAC ATGGAGATCG GTCACCAGTG AAGACATATC CCAAGGACCC CTATCAGGAA 
GAGAAATGGC CCCAGGGATT TGGTCAGCTA ACCAAGGAAG GGATGCTACA GCACTGGGAG 
CTGGGCCAGG CCCTGCGGCA ACGCTACCAT GGCTTTCTGA ACACCTCTTA CCACAGGCAA 
GAGGTTTATG TGCGAAGCAC AGACTTCGAT CGTACTCTCA TGAGTGCTGA GGCCAACCTG 
GCTGGACTCT TCCCTCCCAA TGAAGTTCAG CACTTCAACC CTAACATTTC ATGGCAGCCT 
ATCCCTGTTC ACACTGTGCC CATCACTGAA GACAGGTTGC TGAAGTTTCC TTTGGGTCCA 
TGTCCCCGTT ATGAGCAGCT GCAGAATGAG ACTCGGCAGA CACCAGAGTA TCAGAACAGA 
AGTATTCAGA ATGCACAATT TCTGAACATG GTGGCCAACG AGACAGGGCT TACAAACGTG 
ACCCTAGAGA CCATCTGGAA TGTGTATGAC ACACTCTTTT GTGAGCAAAC CCATGGGCTG 
CTTCTGCCGC CCTGGGCCTC ACCCCAAACC GTGCAGCGTC TGAGCCAGCT AAAGGACTTC 
AGCTTCCTCT TCCTCTTCGG GATCCACGAG CAAGTACAGA AGGCCCGGCT TCAGGGGGGA 
GTTCTGCTGG CTCAGATATT GAAGAACCTG ACCCTAATGG CAACTACCTC TCAATTCCCT 
AAGCTTCTGG TTTATTCCGC GCATGACACT ACCCTGGTTG CTCTGCAAAT GGCATTGAAT 
GTCTACAATG GGAAGCAAGC TCCCTATGCT TCCTGCCACA TATTTGAACT GTACCAGGAA 
GATAATGGGA ATTTCTCAGT CGAGATGTAC TTTCGGAATG ACAGTAAGAA GGCACCCTGG 
CCTCTGATCC TGCCTGGCTG TCCTCACCGT TGCCCACTGC AGGATTTCCT TCGCCTCACA 
GAGCCTGTCA TACCCAAGGA CTGGCAGAAG GAGTGCCAGC TAGCAAATGA TACTGCAGAC 
ACAGAGGTAA TTGTGGCATT GGCTGTCTGT GGCTCCATCC TCTTCCTTCT CATAGTGTTG 
CTCCTCACCA TCCTCTTCCG GATGCAGGCC CAGCCTCCTG GCTACCACCA TGTTGCAGAC 
AGGGAAGACC ATGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237559.1
CDS	16..1233
Translation	MAKAALPASVLTNASGRLQQLYRHGDRSPVKTYPKDPYQEEKWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNEVQHFNPNISWQPIPVHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNRSIQNAQFLNMVANETGLTNVTLETIWNVYDTLFCEQTHGLLLPPWASPQTVQRLSQLKDFSFLFLFGIHEQVQKARLQGGVLLAQILKNLTLMATTSQFPKLLVYSAHDTTLVALQMALNVYNGKQAPYASCHIFELYQEDNGNFSVEMYFRNDSKKAPWPLILPGCPHRCPLQDFLRLTEPVIPKDWQKECQLANDTADTEVIVALAVCGSILFLLIVLLLTILFRMQAQPPGYHHVADREDHA

Target ORF information:

RefSeq Version	XM_011239257.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus acid phosphatase 2, lysosomal (Acp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239257.3

ORF Insert Sequence:

ATGGCGAAGG CTGCACTTCC GGCAAGTGTC CTTACGAATG CATCCGGGCG GCTGCAGCAG 
CTGTATCGAC ATGGAGATCG GTCACCAGTG AAGACATATC CCAAGGACCC CTATCAGGAA 
GAGAAATGGC CCCAGGGATT TGGTCAGCTA ACCAAGGAAG GGATGCTACA GCACTGGGAG 
CTGGGCCAGG CCCTGCGGCA ACGCTACCAT GGCTTTCTGA ACACCTCTTA CCACAGGCAA 
GAGGTTTATG TGCGAAGCAC AGACTTCGAT CGTACTCTCA TGAGTGCTGA GGCCAACCTG 
GCTGGACTCT TCCCTCCCAA TGAAGTTCAG CACTTCAACC CTAACATTTC ATGGCAGCCT 
ATCCCTGTTC ACACTGTGCC CATCACTGAA GACAGGTTGC TGAAGTTTCC TTTGGGTCCA 
TGTCCCCGTT ATGAGCAGCT GCAGAATGAG ACTCGGCAGA CACCAGAGTA TCAGAACAGA 
AGTATTCAGA ATGCACAATT TCTGAACATG GTGGCCAACG AGACAGGGCT TACAAACGTG 
ACCCTAGAGA CCATCTGGAA TGTGTATGAC ACACTCTTTT GTGAGCAAAC CCATGGGCTG 
CTTCTGCCGC CCTGGGCCTC ACCCCAAACC GTGCAGCGTC TGAGCCAGCT AAAGGACTTC 
AGCTTCCTCT TCCTCTTCGG GATCCACGAG CAAGTACAGA AGGCCCGGCT TCAGGGGGGA 
GTTCTGCTGG CTCAGATATT GAAGAACCTG ACCCTAATGG CAACTACCTC TCAATTCCCT 
AAGCTTCTGG TTTATTCCGC GCATGACACT ACCCTGGTTG CTCTGCAAAT GGCATTGAAT 
GTCTACAATG GGAAGCAAGC TCCCTATGCT TCCTGCCACA TATTTGAACT GTACCAGGAA 
GATAATGGGA ATTTCTCAGT CGAGATGTAC TTTCGGAATG ACAGTAAGAA GGCACCCTGG 
CCTCTGATCC TGCCTGGCTG TCCTCACCGT TGCCCACTGC AGGATTTCCT TCGCCTCACA 
GAGCCTGTCA TACCCAAGGA CTGGCAGAAG GAGTGCCAGC TAGCAAATGA TACTGCAGAC 
ACAGAGGTAA TTGTGGCATT GGCTGTCTGT GGCTCCATCC TCTTCCTTCT CATAGTGTTG 
CTCCTCACCA TCCTCTTCCG GATGCAGGCC CAGCCTCCTG GCTACCACCA TGTTGCAGAC 
AGGGAAGACC ATGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence of efficient stop codon readthrough in four mammalian genes.
Nucleic acids research42(14)8928-38(2014 Aug)
Loughran G,Chou MY,Ivanov IP,Jungreis I,Kellis M,Kiran AM,Baranov PV,Atkins JF

Purkinje cell compartmentation in the cerebellum of the lysosomal Acid phosphatase 2 mutant mouse (nax - naked-ataxia mutant mouse).
PloS one9(4)e94327(2014)
Bailey K,Rahimi Balaei M,Mannan A,Del Bigio MR,Marzban H

Spatial and temporal expression of lysosomal acid phosphatase 2 (ACP2) reveals dynamic patterning of the mouse cerebellar cortex.
Cerebellum (London, England)12(6)870-81(2013 Dec)
Bailey K,Rahimi Balaei M,Mehdizadeh M,Marzban H

Mannose 6 dephosphorylation of lysosomal proteins mediated by acid phosphatases Acp2 and Acp5.
Molecular and cellular biology32(4)774-82(2012 Feb)
Makrypidi G,Damme M,M?ller-Loennies S,Trusch M,Schmidt B,Schl?ter H,Heeren J,L?bke T,Saftig P,Braulke T

Increased expression of lysosomal acid phosphatase in CLN3-defective cells and mouse brain tissue.
Journal of neurochemistry103(6)2177-88(2007 Dec)
Pohl S,Mitchison HM,Kohlsch?tter A,van Diggelen O,Braulke T,Storch S

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Acp2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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