Atp6v0b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp6v0b
Entrez Gene ID	114143
Full Name	ATPase, H+ transporting, lysosomal V0 subunit B
Synonyms	2310024H13Rik,Atp6f,VMA16
General protein information	Preferred Names ATPase, H+ transporting, lysosomal V0 subunit B Names V-type proton ATPase 21 kDa proteolipid subunit 23 kDa subunit of V-ATPase ATPase, H+ transporting, V0 subunit B ATPase, H+ transporting, lysosomal (vacuolar proton pump) ATPase, H+ transporting, lysosomal 21kDa, V0 subunit B V-ATPase 21 kDa proteolipid subunit lysosomal 21kDa vacuolar proton pump 21 kDa proteolipid subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_033617.3	NP_291095.1	V-type proton ATPase 21 kDa proteolipid subunit
XM_030253094.1	XP_030108954.1	V-type proton ATPase 21 kDa proteolipid subunit isoform X1
XM_030253095.1	XP_030108955.1	V-type proton ATPase 21 kDa proteolipid subunit isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP6V0B	NP_004038.1
Pan troglodytes (chimpanzee)	ATP6V0B	XP_003308092.1
	ATP6V0B	XP_001097275.1
Bos taurus (cattle)	ATP6V0B	NP_001033127.1
Rattus norvegicus (Norway rat)	Atp6v0b	NP_001100151.1
Drosophila melanogaster (fruit fly)	VhaPPA1-1	NP_001247111.1
Magnaporthe oryzae (rice blast fungus)	MGG_06108	XP_003711974.1
Arabidopsis thaliana (thale cress)	AT4G32530	NP_001119099.1
Gallus gallus (chicken)	ATP6V0B	NP_001264501.1
Caenorhabditis elegans (roundworm)	vha-4	NP_495659.1
Canis lupus familiaris (dog)	ATP6V0B	XP_539645.2
Mus musculus (house mouse)	Atp6v0b	NP_291095.1
Saccharomyces cerevisiae (baker's yeast)	VMA16	NP_011891.1
Schizosaccharomyces pombe (fission yeast)	vma16	NP_594516.1
Xenopus tropicalis (tropical clawed frog)	atp6v0b	NP_001017064.1
Danio rerio (zebrafish)	atp6v0b	NP_955855.1
Neurospora crassa	NCU09747	XP_964449.1
Oryza sativa (rice)	Os01g0610100	NP_001043542.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009334	XP_310004.2
Kluyveromyces lactis	KLLA0E11529g	XP_454470.1
Eremothecium gossypii	AGOS_ACR071W	NP_983473.1
Arabidopsis thaliana (thale cress)	AT2G25610	NP_180132.1

Gene Ontology
Bibliography

Related articles in PubMed

Defects in whirlin, a PDZ domain molecule involved in stereocilia elongation, cause deafness in the whirler mouse and families with DFNB31.
Mburu P, Mustapha M, Varela A, Weil D, El-Amraoui A, Holme RH, Rump A, Hardisty RE, Blanchard S, Coimbra RS, Perfettini I, Parkinson N, Mallon AM, Glenister P, Rogers MJ, Paige AJ, Moir L, Clay J, Rosenthal A, Liu XZ, Blanco G, Steel KP, Petit C, Brown SD
Nature genetics34(4)421-8(2003 Aug)

Expression and localization of the mouse homologue of the yeast V-ATPase 21-kDa Subunit c" (Vma16p).
Nishi T, Kawasaki-Nishi S, Forgac M
The Journal of biological chemistry276(36)34122-30(2001 Sep)

Mouse Atp6f, the gene encoding the 23-kDa proteolipid of vacuolar proton translocating ATPase.
Sun-Wada GH, Murakami H, Nakai H, Wada Y, Futai M
Gene274(1-2)93-9(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Atp6v0b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp6v0b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08960	NM_033617.3 Latest version!	Mus musculus ATPase, H+ transporting, lysosomal V0 subunit B (Atp6v0b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu88390	XM_030253095.1 Latest version!	Mus musculus ATPase, H+ transporting, lysosomal V0 subunit B (Atp6v0b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu88390	XM_030253094.1 Latest version!	Mus musculus ATPase, H+ transporting, lysosomal V0 subunit B (Atp6v0b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp6v0b ( NM_033617.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_291095.1 Mus musculus ATPase, H+ transporting, lysosomal V0 subunit B (Atp6v0b), mRNA.

Price & Availability↑

CloneID	OMu08960
Clone ID Related Accession (Same CDS sequence)	NM_033617.3
Accession Version	NM_033617.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Mus musculus(house mouse)
Product	V-type proton ATPase 21 kDa proteolipid subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL627128.16. On May 1, 2007 this sequence version replaced NM_033617.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC009169.1, AF356006.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGACGGGGC TGGAGTTGCT CTACCTCGGG ATCTTTGTGG CCTTCTGGGC CTGCATGGTC 
GTTGTGGGAA TCTGCTACAC CATCTTTGAC CTGGGCTTTC GCTTTGATGT GGCATGGTTC 
CTGACGGAAA CTTCCCCCTT CATGTGGTCG AATCTGGGCA TTGGCCTAGC AATTTCTCTG 
TCTGTGGTTG GAGCAGCCTG GGGAATCTAT ATAACCGGCT CATCCATTAT TGGGGGTGGG 
GTGAAAGCCC CCAGAATCAA AACCAAGAAC TTGGTTAGTA TTATCTTCTG TGAAGCGGTG 
GCCATCTATG GCATCATCAT GGCAATTGTC ATCAGCAACA TGGCTGAGCC TTTCAGTGCT 
ACGGAGCCCA AGGCCATTGG CCATCGAAAC TACCATGCAG GTTACTCCAT GTTTGGGGCT 
GGCCTCACAG TCGGTCTGTC CAACCTGTTC TGTGGAGTCT GCGTGGGCAT CGTGGGCAGT 
GGGGCCGCCC TGGCGGATGC ACAGAACCCC AGCCTCTTTG TAAAAATTCT CATCGTGGAG 
ATCTTTGGCA GTGCCATTGG CCTCTTTGGG GTCATTGTTG CAATCCTTCA GACCTCCAGA 
GTGAAGATGG GTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_291095.1
CDS	94..711
Translation	MTGLELLYLGIFVAFWACMVVVGICYTIFDLGFRFDVAWFLTETSPFMWSNLGIGLAISLSVVGAAWGIYITGSSIIGGGVKAPRIKTKNLVSIIFCEAVAIYGIIMAIVISNMAEPFSATEPKAIGHRNYHAGYSMFGAGLTVGLSNLFCGVCVGIVGSGAALADAQNPSLFVKILIVEIFGSAIGLFGVIVAILQTSRVKMGD

Target ORF information:

RefSeq Version	NM_033617.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, H+ transporting, lysosomal V0 subunit B (Atp6v0b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033617.3

ORF Insert Sequence:

ATGACGGGGC TGGAGTTGCT CTACCTCGGG ATCTTTGTGG CCTTCTGGGC CTGCATGGTC 
GTTGTGGGAA TCTGCTACAC CATCTTTGAC CTGGGCTTTC GCTTTGATGT GGCATGGTTC 
CTGACGGAAA CTTCCCCCTT CATGTGGTCG AATCTGGGCA TTGGCCTAGC AATTTCTCTG 
TCTGTGGTTG GAGCAGCCTG GGGAATCTAT ATAACCGGCT CATCCATTAT TGGGGGTGGG 
GTGAAAGCCC CCAGAATCAA AACCAAGAAC TTGGTTAGTA TTATCTTCTG TGAAGCGGTG 
GCCATCTATG GCATCATCAT GGCAATTGTC ATCAGCAACA TGGCTGAGCC TTTCAGTGCT 
ACGGAGCCCA AGGCCATTGG CCATCGAAAC TACCATGCAG GTTACTCCAT GTTTGGGGCT 
GGCCTCACAG TCGGTCTGTC CAACCTGTTC TGTGGAGTCT GCGTGGGCAT CGTGGGCAGT 
GGGGCCGCCC TGGCGGATGC ACAGAACCCC AGCCTCTTTG TAAAAATTCT CATCGTGGAG 
ATCTTTGGCA GTGCCATTGG CCTCTTTGGG GTCATTGTTG CAATCCTTCA GACCTCCAGA 
GTGAAGATGG GTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp6v0b ( XM_030253094.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108954.1 Mus musculus ATPase, H+ transporting, lysosomal V0 subunit B (Atp6v0b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu88390
Clone ID Related Accession (Same CDS sequence)	XM_030253094.1 , XM_030253095.1
Accession Version	XM_030253094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	V-type proton ATPase 21 kDa proteolipid subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCGA ATCTGGGCAT TGGCCTAGCA ATTTCTCTGT CTGTGGTTGG AGCAGCCTGG 
GGAATCTATA TAACCGGCTC ATCCATTATT GGGGGTGGGG TGAAAGCCCC CAGAATCAAA 
ACCAAGAACT TGGTTAGTAT TATCTTCTGT GAAGCGGTGG CCATCTATGG CATCATCATG 
GCAATTGTCA TCAGCAACAT GGCTGAGCCT TTCAGTGCTA CGGAGCCCAA GGCCATTGGC 
CATCGAAACT ACCATGCAGG TTACTCCATG TTTGGGGCTG GCCTCACAGT CGGTCTGTCC 
AACCTGTTCT GTGGAGTCTG CGTGGGCATC GTGGGCAGTG GGGCCGCCCT GGCGGATGCA 
CAGAACCCCA GCCTCTTTGT AAAAATTCTC ATCGTGGAGA TCTTTGGCAG TGCCATTGGC 
CTCTTTGGGG TCATTGTTGC AATCCTTCAG ACCTCCAGAG TGAAGATGGG TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108954.1
CDS	644..1120
Translation	MWSNLGIGLAISLSVVGAAWGIYITGSSIIGGGVKAPRIKTKNLVSIIFCEAVAIYGIIMAIVISNMAEPFSATEPKAIGHRNYHAGYSMFGAGLTVGLSNLFCGVCVGIVGSGAALADAQNPSLFVKILIVEIFGSAIGLFGVIVAILQTSRVKMGD

Target ORF information:

RefSeq Version	XM_030253094.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, H+ transporting, lysosomal V0 subunit B (Atp6v0b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253094.1

ORF Insert Sequence:

ATGTGGTCGA ATCTGGGCAT TGGCCTAGCA ATTTCTCTGT CTGTGGTTGG AGCAGCCTGG 
GGAATCTATA TAACCGGCTC ATCCATTATT GGGGGTGGGG TGAAAGCCCC CAGAATCAAA 
ACCAAGAACT TGGTTAGTAT TATCTTCTGT GAAGCGGTGG CCATCTATGG CATCATCATG 
GCAATTGTCA TCAGCAACAT GGCTGAGCCT TTCAGTGCTA CGGAGCCCAA GGCCATTGGC 
CATCGAAACT ACCATGCAGG TTACTCCATG TTTGGGGCTG GCCTCACAGT CGGTCTGTCC 
AACCTGTTCT GTGGAGTCTG CGTGGGCATC GTGGGCAGTG GGGCCGCCCT GGCGGATGCA 
CAGAACCCCA GCCTCTTTGT AAAAATTCTC ATCGTGGAGA TCTTTGGCAG TGCCATTGGC 
CTCTTTGGGG TCATTGTTGC AATCCTTCAG ACCTCCAGAG TGAAGATGGG TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp6v0b ( XM_030253095.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108955.1 Mus musculus ATPase, H+ transporting, lysosomal V0 subunit B (Atp6v0b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu88390
Clone ID Related Accession (Same CDS sequence)	XM_030253094.1 , XM_030253095.1
Accession Version	XM_030253095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	V-type proton ATPase 21 kDa proteolipid subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCGA ATCTGGGCAT TGGCCTAGCA ATTTCTCTGT CTGTGGTTGG AGCAGCCTGG 
GGAATCTATA TAACCGGCTC ATCCATTATT GGGGGTGGGG TGAAAGCCCC CAGAATCAAA 
ACCAAGAACT TGGTTAGTAT TATCTTCTGT GAAGCGGTGG CCATCTATGG CATCATCATG 
GCAATTGTCA TCAGCAACAT GGCTGAGCCT TTCAGTGCTA CGGAGCCCAA GGCCATTGGC 
CATCGAAACT ACCATGCAGG TTACTCCATG TTTGGGGCTG GCCTCACAGT CGGTCTGTCC 
AACCTGTTCT GTGGAGTCTG CGTGGGCATC GTGGGCAGTG GGGCCGCCCT GGCGGATGCA 
CAGAACCCCA GCCTCTTTGT AAAAATTCTC ATCGTGGAGA TCTTTGGCAG TGCCATTGGC 
CTCTTTGGGG TCATTGTTGC AATCCTTCAG ACCTCCAGAG TGAAGATGGG TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108955.1
CDS	295..771
Translation	MWSNLGIGLAISLSVVGAAWGIYITGSSIIGGGVKAPRIKTKNLVSIIFCEAVAIYGIIMAIVISNMAEPFSATEPKAIGHRNYHAGYSMFGAGLTVGLSNLFCGVCVGIVGSGAALADAQNPSLFVKILIVEIFGSAIGLFGVIVAILQTSRVKMGD

Target ORF information:

RefSeq Version	XM_030253095.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, H+ transporting, lysosomal V0 subunit B (Atp6v0b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253095.1

ORF Insert Sequence:

ATGTGGTCGA ATCTGGGCAT TGGCCTAGCA ATTTCTCTGT CTGTGGTTGG AGCAGCCTGG 
GGAATCTATA TAACCGGCTC ATCCATTATT GGGGGTGGGG TGAAAGCCCC CAGAATCAAA 
ACCAAGAACT TGGTTAGTAT TATCTTCTGT GAAGCGGTGG CCATCTATGG CATCATCATG 
GCAATTGTCA TCAGCAACAT GGCTGAGCCT TTCAGTGCTA CGGAGCCCAA GGCCATTGGC 
CATCGAAACT ACCATGCAGG TTACTCCATG TTTGGGGCTG GCCTCACAGT CGGTCTGTCC 
AACCTGTTCT GTGGAGTCTG CGTGGGCATC GTGGGCAGTG GGGCCGCCCT GGCGGATGCA 
CAGAACCCCA GCCTCTTTGT AAAAATTCTC ATCGTGGAGA TCTTTGGCAG TGCCATTGGC 
CTCTTTGGGG TCATTGTTGC AATCCTTCAG ACCTCCAGAG TGAAGATGGG TGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defects in whirlin, a PDZ domain molecule involved in stereocilia elongation, cause deafness in the whirler mouse and families with DFNB31.
Nature genetics34(4)421-8(2003 Aug)
Mburu P,Mustapha M,Varela A,Weil D,El-Amraoui A,Holme RH,Rump A,Hardisty RE,Blanchard S,Coimbra RS,Perfettini I,Parkinson N,Mallon AM,Glenister P,Rogers MJ,Paige AJ,Moir L,Clay J,Rosenthal A,Liu XZ,Blanco G,Steel KP,Petit C,Brown SD

Expression and localization of the mouse homologue of the yeast V-ATPase 21-kDa Subunit c" (Vma16p).
The Journal of biological chemistry276(36)34122-30(2001 Sep)
Nishi T,Kawasaki-Nishi S,Forgac M

Mouse Atp6f, the gene encoding the 23-kDa proteolipid of vacuolar proton translocating ATPase.
Gene274(1-2)93-9(2001 Aug)
Sun-Wada GH,Murakami H,Nakai H,Wada Y,Futai M

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Atp6v0b cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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