Acads cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acads
Entrez Gene ID	11409
Full Name	acyl-Coenzyme A dehydrogenase, short chain
Synonyms	AI196007,Bcd-1,Bcd1,Hdlq8,SCAD
General protein information	Preferred Names acyl-Coenzyme A dehydrogenase, short chain Names short-chain specific acyl-CoA dehydrogenase, mitochondrial butyryl-CoA dehydrogenase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 55.99 cM\|5 F
Summary	This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the Acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum at C(four)-CoA. In mice, deficiency of this gene has been linked to cold sensitivity and increased high-density lipoprotein levels. [provided by RefSeq, Nov 2012].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007383.3	NP_031409.2	short-chain specific acyl-CoA dehydrogenase, mitochondrial precursor

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001951	XP_321112.5
Xenopus tropicalis (tropical clawed frog)	acads	NP_989269.1
Homo sapiens (human)	ACADS	NP_000008.1
Canis lupus familiaris (dog)	ACADS	XP_005636365.1
Mus musculus (house mouse)	Acads	NP_031409.2
	ACADS	XP_001091115.1
Bos taurus (cattle)	ACADS	NP_001029573.1
Gallus gallus (chicken)	ACADS	NP_001006193.1
Danio rerio (zebrafish)	acads	NP_001003743.1
Drosophila melanogaster (fruit fly)	Arc42	NP_650840.1
Pan troglodytes (chimpanzee)	ACADS	XP_001162935.1
Rattus norvegicus (Norway rat)	Acads	NP_071957.1

Gene Ontology
Bibliography

Related articles in PubMed

Myocardial VHL-HIF Signaling Controls an Embryonic Metabolic Switch Essential for Cardiac Maturation.
Menendez-Montes I, Escobar B, Palacios B, G?mez MJ, Izquierdo-Garcia JL, Flores L, Jim?nez-Borreguero LJ, Aragones J, Ruiz-Cabello J, Torres M, Martin-Puig S
Developmental cell39(6)724-739(2016 12)

The Colonic Crypt Protects Stem Cells from Microbiota-Derived Metabolites.
Kaiko GE, Ryu SH, Koues OI, Collins PL, Solnica-Krezel L, Pearce EJ, Pearce EL, Oltz EM, Stappenbeck TS
Cell165(7)1708-1720(2016 Jun)

Complex changes in the liver mitochondrial proteome of short chain acyl-CoA dehydrogenase deficient mice.
Wang W, Mohsen AW, Uechi G, Schreiber E, Balasubramani M, Day B, Michael Barmada M, Vockley J
Molecular genetics and metabolism112(1)30-9(2014 May)

Measurement of tissue acyl-CoAs using flow-injection tandem mass spectrometry: acyl-CoA profiles in short-chain fatty acid oxidation defects.
Palladino AA, Chen J, Kallish S, Stanley CA, Bennett MJ
Molecular genetics and metabolism107(4)679-83(2012 Dec)

Untangling HDL quantitative trait loci on mouse chromosome 5 and identifying Scarb1 and Acads as the underlying genes.
Su Z, Leduc MS, Korstanje R, Paigen B
Journal of lipid research51(9)2706-13(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that the decreased beta-oxidation of short-chain fatty acids in Acads-deficient mice fed HF diet produces a state of energy deficiency in the brain.
Title: Brain transcriptional responses to high-fat diet in Acads-deficient mice reveal energy sensing pathways.

These results suggest that reduced brown adipose tissue function is not the major factor causing cold sensitivity in acyl-CoA dehydrogenase knockout strains.
Title: Brown adipose tissue function in short-chain acyl-CoA dehydrogenase deficient mice.

SCAD-deficient mice display altered postingestive responses to dietary fat, providing further evidence for metabolic control of feeding. Acads does not alter acute orosensory response to this fat stimulus.
Title: Mice bearing Acads mutation display altered postingestive but not 5-s orosensory response to dietary fat.

some variants of SCAD proteins (R22W, G68C, W153R, and R359C) are severely misfolded, which may implicate a role in the pathogenesis of SCAD deficiency
Title: Misfolding, degradation, and aggregation of variant proteins. The molecular pathogenesis of short chain acyl-CoA dehydrogenase (SCAD) deficiency.

The following Acads gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acads cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16150	NM_007383.3 Latest version!	Mus musculus acyl-Coenzyme A dehydrogenase, short chain (Acads), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acads ( NM_007383.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031409.2 Mus musculus acyl-Coenzyme A dehydrogenase, short chain (Acads), mRNA.

Price & Availability↑

CloneID	OMu16150
Clone ID Related Accession (Same CDS sequence)	NM_007383.3
Accession Version	NM_007383.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	short-chain specific acyl-CoA dehydrogenase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY077302.1, AK169428.1 and BE993251.1. On Nov 28, 2012 this sequence version replaced NM_007383.2. Sequence Note: The transit peptide annotated at aa 1-24 is inferred by similarity to the cleavage site in the orthologous rat protein as described in PMID: 2777793. The software program TargetP1.1 predicts that the transit peptide in mouse is aa 1-48. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016259.1, AK155361.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCG CCTTGCTCGC CCGGGCCCGT GGCCCTCTCC GTAGAGCTCT CGGTGTTCGG 
GACTGGCGAC GGTTACACAC TGTTTACCAG TCTGTGGAGC TGCCTGAGAC ACACCAGATG 
TTGCGTCAGA CATGCCGTGA CTTTGCCGAG AAGGAGTTGG TCCCCATTGC GGCCCAGCTG 
GACAGGGAGC ATCTCTTCCC CACAGCTCAG GTTAAGAAGA TGGGTGAGCT CGGGCTGCTG 
GCCATGGATG TGCCAGAGGA GCTGAGTGGT GCAGGCTTGG ATTACCTGGC CTACTCCATC 
GCCCTGGAGG AGATCAGCCG TGCCTGCGCC TCCACGGGAG TTATCATGAG CGTCAACAAT 
TCTCTCTACT TGGGACCCAT TCTGAAGTTT GGATCCGCAC AGCAGAAGCA ACAGTGGATC 
ACCCCTTTCA CCAATGGTGA CAAAATCGGC TGTTTTGCCC TCAGTGAGCC AGGCAATGGC 
AGTGATGCTG GAGCCGCTTC CACCACTGCC CGGGAAGAGG GTGACTCATG GGTCCTCAAC 
GGCACCAAAG CTTGGATCAC CAACTCCTGG GAGGCTTCCG CCACGGTGGT ATTTGCCAGC 
ACAGACAGGT CCCGGCAGAA CAAGGGTATC AGTGCCTTCC TGGTTCCCAT GCCAACTCCT 
GGGCTCACGC TGGGCAAGAA GGAAGACAAG CTGGGCATCC GGGCCTCCTC CACAGCTAAC 
CTCATCTTTG AGGACTGCCG GATCCCCAAG GAGAACCTGC TTGGGGAGCC TGGGATGGGC 
TTCAAAATAG CCATGCAAAC CCTGGACATG GGTCGCATTG GCATCGCCTC CCAGGCCCTG 
GGCATCGCCC AGGCCTCCCT GGATTGTGCT GTGAAGTATG CCGAGAACCG CAATGCCTTT 
GGGGCACCGC TCACCAAGCT CCAAAATATC CAGTTCAAGC TGGCAGACAT GGCCCTGGCC 
CTGGAGAGTG CCCGCCTGCT GACCTGGCGT GCTGCCATGT TGAAAGACAA CAAGAAACCT 
TTCACCAAGG AGTCCGCCAT GGCCAAACTG GCTGCATCGG AGGCTGCAAC CGCCATTAGC 
CACCAGGCCA TCCAGATCCT GGGCGGCATG GGGTATGTGA CAGAGATGCC GGCTGAGCGG 
TACTACCGAG ATGCCCGCAT CACTGAGATC TACGAAGGGA CCAGCGAAAT CCAGAGACTG 
GTGATCGCTG GGCATCTGCT CCGGAGCTAC CGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031409.2
CDS	77..1315
Translation	MAAALLARARGPLRRALGVRDWRRLHTVYQSVELPETHQMLRQTCRDFAEKELVPIAAQLDREHLFPTAQVKKMGELGLLAMDVPEELSGAGLDYLAYSIALEEISRACASTGVIMSVNNSLYLGPILKFGSAQQKQQWITPFTNGDKIGCFALSEPGNGSDAGAASTTAREEGDSWVLNGTKAWITNSWEASATVVFASTDRSRQNKGISAFLVPMPTPGLTLGKKEDKLGIRASSTANLIFEDCRIPKENLLGEPGMGFKIAMQTLDMGRIGIASQALGIAQASLDCAVKYAENRNAFGAPLTKLQNIQFKLADMALALESARLLTWRAAMLKDNKKPFTKESAMAKLAASEAATAISHQAIQILGGMGYVTEMPAERYYRDARITEIYEGTSEIQRLVIAGHLLRSYRS

Target ORF information:

RefSeq Version	NM_007383.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-Coenzyme A dehydrogenase, short chain (Acads), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007383.3

ORF Insert Sequence:

ATGGCTGCCG CCTTGCTCGC CCGGGCCCGT GGCCCTCTCC GTAGAGCTCT CGGTGTTCGG 
GACTGGCGAC GGTTACACAC TGTTTACCAG TCTGTGGAGC TGCCTGAGAC ACACCAGATG 
TTGCGTCAGA CATGCCGTGA CTTTGCCGAG AAGGAGTTGG TCCCCATTGC GGCCCAGCTG 
GACAGGGAGC ATCTCTTCCC CACAGCTCAG GTTAAGAAGA TGGGTGAGCT CGGGCTGCTG 
GCCATGGATG TGCCAGAGGA GCTGAGTGGT GCAGGCTTGG ATTACCTGGC CTACTCCATC 
GCCCTGGAGG AGATCAGCCG TGCCTGCGCC TCCACGGGAG TTATCATGAG CGTCAACAAT 
TCTCTCTACT TGGGACCCAT TCTGAAGTTT GGATCCGCAC AGCAGAAGCA ACAGTGGATC 
ACCCCTTTCA CCAATGGTGA CAAAATCGGC TGTTTTGCCC TCAGTGAGCC AGGCAATGGC 
AGTGATGCTG GAGCCGCTTC CACCACTGCC CGGGAAGAGG GTGACTCATG GGTCCTCAAC 
GGCACCAAAG CTTGGATCAC CAACTCCTGG GAGGCTTCCG CCACGGTGGT ATTTGCCAGC 
ACAGACAGGT CCCGGCAGAA CAAGGGTATC AGTGCCTTCC TGGTTCCCAT GCCAACTCCT 
GGGCTCACGC TGGGCAAGAA GGAAGACAAG CTGGGCATCC GGGCCTCCTC CACAGCTAAC 
CTCATCTTTG AGGACTGCCG GATCCCCAAG GAGAACCTGC TTGGGGAGCC TGGGATGGGC 
TTCAAAATAG CCATGCAAAC CCTGGACATG GGTCGCATTG GCATCGCCTC CCAGGCCCTG 
GGCATCGCCC AGGCCTCCCT GGATTGTGCT GTGAAGTATG CCGAGAACCG CAATGCCTTT 
GGGGCACCGC TCACCAAGCT CCAAAATATC CAGTTCAAGC TGGCAGACAT GGCCCTGGCC 
CTGGAGAGTG CCCGCCTGCT GACCTGGCGT GCTGCCATGT TGAAAGACAA CAAGAAACCT 
TTCACCAAGG AGTCCGCCAT GGCCAAACTG GCTGCATCGG AGGCTGCAAC CGCCATTAGC 
CACCAGGCCA TCCAGATCCT GGGCGGCATG GGGTATGTGA CAGAGATGCC GGCTGAGCGG 
TACTACCGAG ATGCCCGCAT CACTGAGATC TACGAAGGGA CCAGCGAAAT CCAGAGACTG 
GTGATCGCTG GGCATCTGCT CCGGAGCTAC CGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Myocardial VHL-HIF Signaling Controls an Embryonic Metabolic Switch Essential for Cardiac Maturation.
Developmental cell39(6)724-739(2016 12)
Menendez-Montes I,Escobar B,Palacios B,G?mez MJ,Izquierdo-Garcia JL,Flores L,Jim?nez-Borreguero LJ,Aragones J,Ruiz-Cabello J,Torres M,Martin-Puig S

The Colonic Crypt Protects Stem Cells from Microbiota-Derived Metabolites.
Cell165(7)1708-1720(2016 Jun)
Kaiko GE,Ryu SH,Koues OI,Collins PL,Solnica-Krezel L,Pearce EJ,Pearce EL,Oltz EM,Stappenbeck TS

Complex changes in the liver mitochondrial proteome of short chain acyl-CoA dehydrogenase deficient mice.
Molecular genetics and metabolism112(1)30-9(2014 May)
Wang W,Mohsen AW,Uechi G,Schreiber E,Balasubramani M,Day B,Michael Barmada M,Vockley J

Measurement of tissue acyl-CoAs using flow-injection tandem mass spectrometry: acyl-CoA profiles in short-chain fatty acid oxidation defects.
Molecular genetics and metabolism107(4)679-83(2012 Dec)
Palladino AA,Chen J,Kallish S,Stanley CA,Bennett MJ

Untangling HDL quantitative trait loci on mouse chromosome 5 and identifying Scarb1 and Acads as the underlying genes.
Journal of lipid research51(9)2706-13(2010 Sep)
Su Z,Leduc MS,Korstanje R,Paigen B

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Acads cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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