Acadm cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acadm
Entrez Gene ID	11364
Full Name	acyl-Coenzyme A dehydrogenase, medium chain
Synonyms	AU018656,MCAD
General protein information	Preferred Names acyl-Coenzyme A dehydrogenase, medium chain Names medium-chain specific acyl-CoA dehydrogenase, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 H3\|3 78.77 cM
Summary	This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C6- and C12-acylCoA. In mice, deficiency of this gene can cause neonatal mortality as well as fasting and cold intolerance. This gene has multiple, intronless pseudogenes. [provided by RefSeq, Nov 2012].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007382.5	NP_031408.1	medium-chain specific acyl-CoA dehydrogenase, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACADM	NP_000007.1
Bos taurus (cattle)	ACADM	NP_001068703.1
Rattus norvegicus (Norway rat)	Acadm	NP_058682.2
	ACADM	XP_001101274.1
Drosophila melanogaster (fruit fly)	CG12262	NP_648149.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005662	XP_315683.2
Pan troglodytes (chimpanzee)	ACADM	NP_001104286.1
Canis lupus familiaris (dog)	ACADM	XP_005622188.1
Caenorhabditis elegans (roundworm)	acdh-8	NP_495142.1
Mus musculus (house mouse)	Acadm	NP_031408.1
Danio rerio (zebrafish)	acadm	NP_998175.2
Caenorhabditis elegans (roundworm)	acdh-7	NP_510789.1
Xenopus tropicalis (tropical clawed frog)	acadm	XP_002936129.2

Gene Ontology
Bibliography

Related articles in PubMed

Cardiac Med1 deletion promotes early lethality, cardiac remodeling, and transcriptional reprogramming.
Spitler KM, Ponce JM, Oudit GY, Hall DD, Grueter CE
American journal of physiology. Heart and circulatory physiology312(4)H768-H780(2017 Apr)

Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose Tissue.
Duteil D, Tosic M, Lausecker F, Nenseth HZ, M?ller JM, Urban S, Willmann D, Petroll K, Messaddeq N, Arrigoni L, Manke T, Kornfeld JW, Br?ning JC, Zagoriy V, Meret M, Dengjel J, Kanouni T, Sch?le R
Cell reports17(4)1008-1021(2016 10)

Targeted disruption of mouse long-chain acyl-CoA dehydrogenase gene reveals crucial roles for fatty acid oxidation.
Kurtz DM, Rinaldo P, Rhead WJ, Tian L, Millington DS, Vockley J, Hamm DA, Brix AE, Lindsey JR, Pinkert CA, O'Brien WE, Wood PA
Proceedings of the National Academy of Sciences of the United States of America95(26)15592-7(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A high-fat diet increases adiposity but maintains mitochondrial oxidative enzymes (MCAD/citrate synthase) without affecting development of heart failure with pressure overload.
Title: A high-fat diet increases adiposity but maintains mitochondrial oxidative enzymes without affecting development of heart failure with pressure overload.

MCAD deficiency in mice leads to specific changes in hepatic carbohydrate management on exposure to metabolic stress.
Title: Disturbed hepatic carbohydrate management during high metabolic demand in medium-chain acyl-CoA dehydrogenase (MCAD)-deficient mice.

MCAD is reduced in liver, heart & kidney in lipopolysaccharide-induced acute phase response; binding liver nuclear extracts to ERRalpha response element found in promoter region of MCAD was decreased during APR, suggesting less transcription of MCAD
Title: Suppression of estrogen-related receptor alpha and medium-chain acyl-coenzyme A dehydrogenase in the acute-phase response.

The MCAD-/- mice developed an organic aciduria and fatty liver, and showed profound cold intolerance at 4 degrees C with prior fasting.
Title: Medium-chain acyl-CoA dehydrogenase deficiency in gene-targeted mice.

the transcriptional regulatory circuits involved in the control of MCAD gene expression under hypoxic conditions are modulated by upstream factors that are sensitive to the levels of oxygen
Title: Counter-regulatory effects of incremental hypoxia on the transcription of a cardiac fatty acid oxidation enzyme-encoding gene.

The following Acadm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acadm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15990	NM_007382.5 Latest version!	Mus musculus acyl-Coenzyme A dehydrogenase, medium chain (Acadm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acadm ( NM_007382.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031408.1 Mus musculus acyl-Coenzyme A dehydrogenase, medium chain (Acadm), mRNA.

Price & Availability↑

CloneID	OMu15990
Clone ID Related Accession (Same CDS sequence)	NM_007382.5
Accession Version	NM_007382.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	medium-chain specific acyl-CoA dehydrogenase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087184.7, CX232934.1, AK146444.1 and BB745012.1. On Nov 28, 2012 this sequence version replaced NM_007382.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The transit peptide annotated at aa 1-25 is inferred by similarity to the cleavage site in the orthologous rat protein as described in PMID: 2777793. The software program TargetP1.1 predicts that the transit peptide in mouse is aa 1-27. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146444.1, U07159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849390, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCGG CGTTCCGCAG AGGCTGCAGG GTCCTGAGAA GTGTTTCTCA TTTTGAGTGT 
CGAACACAAC ACTCGAAAGC GGCTCACAAG CAGGAGCCCG GATTAGGGTT TAGTTTTGAG 
TTGACGGAAC AGCAGAAAGA GTTTCAAGCA ACTGCCCGCA AGTTTGCCAG AGAGGAGATT 
ATCCCCGTCG CCCCGGAATA TGACAAAAGC GGGGAGTACC CGTTCCCTCT CATCAAAAGA 
GCCTGGGAAC TCGGCTTGAT CAACGCGCAC ATTCCGGAAA GTTGCGGTGG CCTTGGCCTG 
GGAACGTTCG ATGCTTGTTT AATTACCGAA GAGTTGGCGT ATGGGTGTAC AGGGGTGCAA 
ACTGCTATTG AAGCAAATTC TTTGGGGCAA ATGCCTGTGA TTCTTGCTGG AAATGATCAA 
CAAAAAAAGA AGTATTTGGG GAGGATGACG GAGCAGCCAA TGATGTGTGC TTACTGTGTG 
ACAGAGCCCT CCGCAGGCTC TGATGTGGCG GCCATTAAGA CCAAAGCAGA GAAGAAGGGT 
GACGAGTATG TTATCAACGG CCAGAAGATG TGGATAACCA ACGGGGGAAA GGCCAACTGG 
TATTTCTTGT TGGCACGTTC TAACCCAGAT CCTAAAGTAC CCGCTAGTAA AGCCTTTACT 
GGATTCATTG TGGAAGCCGA CACCCCGGGA ATACATATTG GAAAAAAGGA ACTAAACATG 
GGCCAGCGAT GCTCTGACAC CAGAGGAATT GCTTTCGAAG ACGTCAGAGT GCCTAAGGAA 
AATGTGTTAA TCGGTGAAGG AGCAGGTTTC AAGATCGCAA TGGGTGCTTT TGATAGAACC 
AGACCTACAG TCGCAGCTGG CGCTGTCGGG CTAGCCCAGA GAGCTCTAGA CGAAGCCACG 
AAGTATGCCC TGGATAGGAA GACATTTGGA AAGCTGCTAG TGGAGCACCA AGGAGTTTCA 
TTTCTGCTCG CAGAAATGGC GATGAAGGTT GAACTCGCTA GGCTCAGTTA CCAGAGAGCA 
GCCTGGGAGG TTGACTCCGG TCGCCGGAAC ACTTACTATG CCTCGATTGC AAAGGCCTTT 
GCTGGAGACA TTGCCAATCA GCTAGCCACT GACGCCGTGC AGATTTTCGG AGGCTATGGA 
TTCAACACAG AGTACCCTGT GGAGAAGCTG ATGAGGGACG CCAAGATCTA TCAGATTTAT 
GAAGGTACTG CACAAATTCA GAGGCTGATC ATAGCTCGTG AGCACATTGA AAAGTATAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031408.1
CDS	219..1484
Translation	MAAAFRRGCRVLRSVSHFECRTQHSKAAHKQEPGLGFSFELTEQQKEFQATARKFAREEIIPVAPEYDKSGEYPFPLIKRAWELGLINAHIPESCGGLGLGTFDACLITEELAYGCTGVQTAIEANSLGQMPVILAGNDQQKKKYLGRMTEQPMMCAYCVTEPSAGSDVAAIKTKAEKKGDEYVINGQKMWITNGGKANWYFLLARSNPDPKVPASKAFTGFIVEADTPGIHIGKKELNMGQRCSDTRGIAFEDVRVPKENVLIGEGAGFKIAMGAFDRTRPTVAAGAVGLAQRALDEATKYALDRKTFGKLLVEHQGVSFLLAEMAMKVELARLSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQIFGGYGFNTEYPVEKLMRDAKIYQIYEGTAQIQRLIIAREHIEKYKN

Target ORF information:

RefSeq Version	NM_007382.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus acyl-Coenzyme A dehydrogenase, medium chain (Acadm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007382.5

ORF Insert Sequence:

ATGGCAGCGG CGTTCCGCAG AGGCTGCAGG GTCCTGAGAA GTGTTTCTCA TTTTGAGTGT 
CGAACACAAC ACTCGAAAGC GGCTCACAAG CAGGAGCCCG GATTAGGGTT TAGTTTTGAG 
TTGACGGAAC AGCAGAAAGA GTTTCAAGCA ACTGCCCGCA AGTTTGCCAG AGAGGAGATT 
ATCCCCGTCG CCCCGGAATA TGACAAAAGC GGGGAGTACC CGTTCCCTCT CATCAAAAGA 
GCCTGGGAAC TCGGCTTGAT CAACGCGCAC ATTCCGGAAA GTTGCGGTGG CCTTGGCCTG 
GGAACGTTCG ATGCTTGTTT AATTACCGAA GAGTTGGCGT ATGGGTGTAC AGGGGTGCAA 
ACTGCTATTG AAGCAAATTC TTTGGGGCAA ATGCCTGTGA TTCTTGCTGG AAATGATCAA 
CAAAAAAAGA AGTATTTGGG GAGGATGACG GAGCAGCCAA TGATGTGTGC TTACTGTGTG 
ACAGAGCCCT CCGCAGGCTC TGATGTGGCG GCCATTAAGA CCAAAGCAGA GAAGAAGGGT 
GACGAGTATG TTATCAACGG CCAGAAGATG TGGATAACCA ACGGGGGAAA GGCCAACTGG 
TATTTCTTGT TGGCACGTTC TAACCCAGAT CCTAAAGTAC CCGCTAGTAA AGCCTTTACT 
GGATTCATTG TGGAAGCCGA CACCCCGGGA ATACATATTG GAAAAAAGGA ACTAAACATG 
GGCCAGCGAT GCTCTGACAC CAGAGGAATT GCTTTCGAAG ACGTCAGAGT GCCTAAGGAA 
AATGTGTTAA TCGGTGAAGG AGCAGGTTTC AAGATCGCAA TGGGTGCTTT TGATAGAACC 
AGACCTACAG TCGCAGCTGG CGCTGTCGGG CTAGCCCAGA GAGCTCTAGA CGAAGCCACG 
AAGTATGCCC TGGATAGGAA GACATTTGGA AAGCTGCTAG TGGAGCACCA AGGAGTTTCA 
TTTCTGCTCG CAGAAATGGC GATGAAGGTT GAACTCGCTA GGCTCAGTTA CCAGAGAGCA 
GCCTGGGAGG TTGACTCCGG TCGCCGGAAC ACTTACTATG CCTCGATTGC AAAGGCCTTT 
GCTGGAGACA TTGCCAATCA GCTAGCCACT GACGCCGTGC AGATTTTCGG AGGCTATGGA 
TTCAACACAG AGTACCCTGT GGAGAAGCTG ATGAGGGACG CCAAGATCTA TCAGATTTAT 
GAAGGTACTG CACAAATTCA GAGGCTGATC ATAGCTCGTG AGCACATTGA AAAGTATAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiac Med1 deletion promotes early lethality, cardiac remodeling, and transcriptional reprogramming.
American journal of physiology. Heart and circulatory physiology312(4)H768-H780(2017 Apr)
Spitler KM,Ponce JM,Oudit GY,Hall DD,Grueter CE

Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose Tissue.
Cell reports17(4)1008-1021(2016 10)
Duteil D,Tosic M,Lausecker F,Nenseth HZ,M?ller JM,Urban S,Willmann D,Petroll K,Messaddeq N,Arrigoni L,Manke T,Kornfeld JW,Br?ning JC,Zagoriy V,Meret M,Dengjel J,Kanouni T,Sch?le R

Targeted disruption of mouse long-chain acyl-CoA dehydrogenase gene reveals crucial roles for fatty acid oxidation.
Proceedings of the National Academy of Sciences of the United States of America95(26)15592-7(1998 Dec)
Kurtz DM,Rinaldo P,Rhead WJ,Tian L,Millington DS,Vockley J,Hamm DA,Brix AE,Lindsey JR,Pinkert CA,O'Brien WE,Wood PA

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Acadm cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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