Ptpa cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptpa
Entrez Gene ID	110854
Full Name	protein phosphatase 2 protein activator
Synonyms	2610042B21Rik,C77440,N28142,PR53,Ppp2r4
General protein information	Preferred Names protein phosphatase 2 protein activator Names serine/threonine-protein phosphatase 2A activator PP2A, subunit B', PR53 isoform phosphotyrosyl phosphatase activator protein phosphatase 2A activator, regulatory subunit B protein phosphatase 2A, regulatory subunit B (PR 53) serine/threonine-protein phosphatase 2A regulatory subunit 4 serine/threonine-protein phosphatase 2A regulatory subunit B'
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 B\|2 21.71 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006497608.2	XP_006497671.1	serine/threonine-protein phosphatase 2A activator isoform X1
NM_138748.5	NP_620087.3	serine/threonine-protein phosphatase 2A activator isoform 2
NM_001362733.1	NP_001349662.1	serine/threonine-protein phosphatase 2A activator isoform 1
NM_001362734.1	NP_001349663.1	serine/threonine-protein phosphatase 2A activator isoform 3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ppp2r4	NP_001102047.1
Gallus gallus (chicken)	PPP2R4	NP_001026542.1
Neurospora crassa	NCU03269	XP_964422.1
Canis lupus familiaris (dog)	PPP2R4	XP_548424.4
Danio rerio (zebrafish)	ppp2r4	NP_001003425.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008652	XP_314749.4
Caenorhabditis elegans (roundworm)	Y71H2AM.20	NP_497617.1
Kluyveromyces lactis	KLLA0F11187g	XP_455589.1
Xenopus tropicalis (tropical clawed frog)	ppp2r4	XP_004916736.1
Pan troglodytes (chimpanzee)	PPP2R4	XP_001163459.2
Magnaporthe oryzae (rice blast fungus)	MGG_09935	XP_003710037.1
Homo sapiens (human)	PPP2R4	NP_821067.1
Bos taurus (cattle)	PPP2R4	NP_001039638.1
Drosophila melanogaster (fruit fly)	Ptpa	NP_523466.2
Eremothecium gossypii	AGOS_ACR139C	NP_983541.1
Oryza sativa (rice)	Os06g0219800	NP_001057165.1
Mus musculus (house mouse)	Ppp2r4	NP_620087.3
Saccharomyces cerevisiae (baker's yeast)	RRD2	NP_015173.1
Schizosaccharomyces pombe (fission yeast)	SPAC1782.05	NP_594712.1
Arabidopsis thaliana (thale cress)	AT4G08960	NP_567342.1

Gene Ontology
Bibliography

Related articles in PubMed

PP2A Inactivation Mediated by
Sents W, Meeusen B, Kalev P, Radaelli E, Sagaert X, Miermans E, Haesen D, Lambrecht C, Dewerchin M, Carmeliet P, Westermarck J, Sablina A, Janssens V
Cancer research77(24)6825-6837(2017 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

A ternary complex comprising FAK, PTP? and IP3 receptor 1 functionally engages focal adhesions and the endoplasmic reticulum to mediate IL-1-induced Ca2+ signalling in fibroblasts.
Wang Q, Wang Y, Downey GP, Plotnikov S, McCulloch CA
The Biochemical journal473(4)397-410(2016 Feb)

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Lewandowski JP, Du F, Zhang S, Powell MB, Falkenstein KN, Ji H, Vokes SA
Developmental biology406(1)92-103(2015 Oct)

Phosphorylation status of nuclear ribosomal protein S3 is reciprocally regulated by protein kinase C{delta} and protein phosphatase 2A.
Kim TS, Kim HD, Shin HS, Kim J
The Journal of biological chemistry284(32)21201-8(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Evidence for PPP2R4 as a haploinsufficient tumor suppressor gene, defining a high-penetrance genetic mechanism for PP2A inhibition in human cancer.
Title: PP2A Inactivation Mediated by

data indicate that PTPalpha and FAK, which are enriched in FAs, interact with IP3R1 at adjacent ER sites to spatially sequester IL-1-induced Ca(2+) signalling
Title: A ternary complex comprising FAK, PTP? and IP3 receptor 1 functionally engages focal adhesions and the endoplasmic reticulum to mediate IL-1-induced Ca2+ signalling in fibroblasts.

oxidative stress regulates the phosphorylation status of nonribosomal rpS3 by both activating PKCdelta and blocking the PP2A interaction with rpS3
Title: Phosphorylation status of nuclear ribosomal protein S3 is reciprocally regulated by protein kinase C{delta} and protein phosphatase 2A.

findings show that ectopic expression of the phosphotyrosyl phosphatase activator PTPA induces cell death in mammalian cells via a mechanism involving caspase-3-dependent apoptosis
Title: Overexpression of the cis/trans isomerase PTPA triggers caspase 3-dependent apoptosis.

The following Ptpa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptpa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10889	NM_138748.5 Latest version!	Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu29241	NM_001362734.1 Latest version!	Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu29241	XM_006497608.2 Latest version!	Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu84339	NM_001362733.1 Latest version!	Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptpa ( NM_138748.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_620087.3 Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu10889
Clone ID Related Accession (Same CDS sequence)	NM_138748.5
Accession Version	NM_138748.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 2A activator isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954299.13 and AK049184.1. On Jul 23, 2009 this sequence version replaced NM_138748.4. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK031591.1, AK150029.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG GCGAGCGGCA GCCGCCGCCA GATTCTTCAG AAGAGACCCC TCCAACTACT 
CAGAACTTTA TCATTCCAAA AAAGGAGATC CACACAGTTC CAGATATGGG CAAATGGAAG 
CGCTCTCAGG CATATGCTGA CTACATTGGC TTCATCCTTA CCCTCAATGA AGGTGTGAAG 
GGGAAGAAGC TGACCTTCGA CTACAAAGTC TCTGAGGCCA TCGAGAAGCT GGTGGCACTT 
CTTGATACGC TGGATAGGTG GATTGATGAA ACCCCGCCAG TGGACCAGCC TTCCCGGTTT 
GGGAACAAAG CCTACAGAAC CTGGTATGCC AAACTTGATC AGGAAGCAGA AAACTTGGTG 
GCCACAGTGG TCCCCACCCA CCTGGCTGCT GCTGTGCCTG AAGTGGCAGT TTACCTGAAG 
GAGGCTGTGG GGAACTCCAC ACGAATTGAC TATGGCACAG GGCATGAGGC TGCCTTTGCT 
GCTTTCCTCT GTTGTCTCTG CAAGATTGGT GTACTCCGGG TGGACGACCA GGTGGCTATT 
GTCTTCAAGG TGTTTGATAG GTATCTTGAG GTTATGCGGA AGTTGCAGAA GACATACAGG 
ATGGAGCCTG CAGGCAGCCA GGGCGTATGG GGTCTGGATG ACTTCCAGTT CCTGCCCTTC 
ATCTGGGGCA GCTCACAGCT CATAGACCAC CCCCACCTGG AGCCCAGACA TTTCGTGGAT 
GAGAAGGCGG TGAGCGAGAA CCACAAGGAC TACATGTTTC TCCAGTGCAT CCTGTTCATC 
ACTGAGATGA AGACTGGCCC CTTTGCGGAA CACTCCAACC AGCTGTGGAA CATCAGTGCT 
GTCCCCTCCT GGTCTAAAGT GAACCAGGGC CTCATTCGAA TGTATAAGGC AGAGTGCCTG 
GAGAAGTTCC CTGTGATCCA GCACTTCAAG TTCGGGAGCC TGCTGCCCAT CCATCCCGTC 
ACATCAGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620087.3
CDS	253..1224
Translation	MAEGERQPPPDSSEETPPTTQNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFDYKVSEAIEKLVALLDTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDQEAENLVATVVPTHLAAAVPEVAVYLKEAVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQVAIVFKVFDRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPHLEPRHFVDEKAVSENHKDYMFLQCILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG

Target ORF information:

RefSeq Version	NM_138748.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138748.5

ORF Insert Sequence:

ATGGCCGAGG GCGAGCGGCA GCCGCCGCCA GATTCTTCAG AAGAGACCCC TCCAACTACT 
CAGAACTTTA TCATTCCAAA AAAGGAGATC CACACAGTTC CAGATATGGG CAAATGGAAG 
CGCTCTCAGG CATATGCTGA CTACATTGGC TTCATCCTTA CCCTCAATGA AGGTGTGAAG 
GGGAAGAAGC TGACCTTCGA CTACAAAGTC TCTGAGGCCA TCGAGAAGCT GGTGGCACTT 
CTTGATACGC TGGATAGGTG GATTGATGAA ACCCCGCCAG TGGACCAGCC TTCCCGGTTT 
GGGAACAAAG CCTACAGAAC CTGGTATGCC AAACTTGATC AGGAAGCAGA AAACTTGGTG 
GCCACAGTGG TCCCCACCCA CCTGGCTGCT GCTGTGCCTG AAGTGGCAGT TTACCTGAAG 
GAGGCTGTGG GGAACTCCAC ACGAATTGAC TATGGCACAG GGCATGAGGC TGCCTTTGCT 
GCTTTCCTCT GTTGTCTCTG CAAGATTGGT GTACTCCGGG TGGACGACCA GGTGGCTATT 
GTCTTCAAGG TGTTTGATAG GTATCTTGAG GTTATGCGGA AGTTGCAGAA GACATACAGG 
ATGGAGCCTG CAGGCAGCCA GGGCGTATGG GGTCTGGATG ACTTCCAGTT CCTGCCCTTC 
ATCTGGGGCA GCTCACAGCT CATAGACCAC CCCCACCTGG AGCCCAGACA TTTCGTGGAT 
GAGAAGGCGG TGAGCGAGAA CCACAAGGAC TACATGTTTC TCCAGTGCAT CCTGTTCATC 
ACTGAGATGA AGACTGGCCC CTTTGCGGAA CACTCCAACC AGCTGTGGAA CATCAGTGCT 
GTCCCCTCCT GGTCTAAAGT GAACCAGGGC CTCATTCGAA TGTATAAGGC AGAGTGCCTG 
GAGAAGTTCC CTGTGATCCA GCACTTCAAG TTCGGGAGCC TGCTGCCCAT CCATCCCGTC 
ACATCAGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptpa ( NM_001362734.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349663.1 Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu29241
Clone ID Related Accession (Same CDS sequence)	XM_006497608.2 , NM_001362734.1
Accession Version	NM_001362734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 2A activator isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954299.13. On Apr 26, 2018 this sequence version replaced XM_006497608.2. Transcript Variant: This variant (3) uses an alternate in-frame splice junction and lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.69898.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG GCGAGCGGCA GCCGCCGCCA GATTCTTCAG AAGAGACCCC TCCAACTACT 
CAGAACTTTA TCATTCCAAA AAAGGAGATC CACACAGTTC CAGATATGGG CAAATGGAAG 
CGCTCTCAGG CATATGCTGA CTACATTGGC TTCATCCTTA CCCTCAATGA AGGTGTGAAG 
GGGAAGAAGC TGACCTTCGA CTACAAAGTC TCTGAGGCCA TCGAGAAGCT GGTGGCACTT 
CTTGATACGC TGGATAGGTG GATTGATGAA ACCCCGCCAG TGGACCAGCC TTCCCGGTTT 
GGGAACAAAG CCTACAGAAC CTGGTATGCC AAACTTGATC AGGAAGCAGA AAACTTGGTG 
GCCACAGTGG TCCCCACCCA CCTGGCTGCT GCTGTGCCTG AAGTGGCAGT TTACCTGAAG 
GAGGCTGTGG GGAACTCCAC ACGAATTGAC TATGGCACAG GGCATGAGGC TGCCTTTGCT 
GCTTTCCTCT GTTGTCTCTG CAAGATTGGT GTACTCCGGG TGGACGACCA GGTGGCTATT 
GTCTTCAAGG TGTTTGATAG GTATCTTGAG GTTATGCGGA AGTTGCAGAA GACATACAGG 
ATGGAGCCTG CAGGCAGCCA GGGCGTATGG GGTCTGGATG ACTTCCAGTT CCTGCCCTTC 
ATCTGGGGCA GCTCACAGCT CATAGATGAA GACTGGCCCC TTTGCGGAAC ACTCCAACCA 
GCTGTGGAAC ATCAGTGCTG TCCCCTCCTG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349663.1
CDS	253..1008
Translation	MAEGERQPPPDSSEETPPTTQNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFDYKVSEAIEKLVALLDTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDQEAENLVATVVPTHLAAAVPEVAVYLKEAVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQVAIVFKVFDRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDEDWPLCGTLQPAVEHQCCPLLV

Target ORF information:

RefSeq Version	NM_001362734.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362734.1

ORF Insert Sequence:

ATGGCCGAGG GCGAGCGGCA GCCGCCGCCA GATTCTTCAG AAGAGACCCC TCCAACTACT 
CAGAACTTTA TCATTCCAAA AAAGGAGATC CACACAGTTC CAGATATGGG CAAATGGAAG 
CGCTCTCAGG CATATGCTGA CTACATTGGC TTCATCCTTA CCCTCAATGA AGGTGTGAAG 
GGGAAGAAGC TGACCTTCGA CTACAAAGTC TCTGAGGCCA TCGAGAAGCT GGTGGCACTT 
CTTGATACGC TGGATAGGTG GATTGATGAA ACCCCGCCAG TGGACCAGCC TTCCCGGTTT 
GGGAACAAAG CCTACAGAAC CTGGTATGCC AAACTTGATC AGGAAGCAGA AAACTTGGTG 
GCCACAGTGG TCCCCACCCA CCTGGCTGCT GCTGTGCCTG AAGTGGCAGT TTACCTGAAG 
GAGGCTGTGG GGAACTCCAC ACGAATTGAC TATGGCACAG GGCATGAGGC TGCCTTTGCT 
GCTTTCCTCT GTTGTCTCTG CAAGATTGGT GTACTCCGGG TGGACGACCA GGTGGCTATT 
GTCTTCAAGG TGTTTGATAG GTATCTTGAG GTTATGCGGA AGTTGCAGAA GACATACAGG 
ATGGAGCCTG CAGGCAGCCA GGGCGTATGG GGTCTGGATG ACTTCCAGTT CCTGCCCTTC 
ATCTGGGGCA GCTCACAGCT CATAGATGAA GACTGGCCCC TTTGCGGAAC ACTCCAACCA 
GCTGTGGAAC ATCAGTGCTG TCCCCTCCTG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptpa ( XM_006497608.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497671.1 Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu29241
Clone ID Related Accession (Same CDS sequence)	XM_006497608.2 , NM_001362734.1
Accession Version	XM_006497608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 2A activator isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497608.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG GCGAGCGGCA GCCGCCGCCA GATTCTTCAG AAGAGACCCC TCCAACTACT 
CAGAACTTTA TCATTCCAAA AAAGGAGATC CACACAGTTC CAGATATGGG CAAATGGAAG 
CGCTCTCAGG CATATGCTGA CTACATTGGC TTCATCCTTA CCCTCAATGA AGGTGTGAAG 
GGGAAGAAGC TGACCTTCGA CTACAAAGTC TCTGAGGCCA TCGAGAAGCT GGTGGCACTT 
CTTGATACGC TGGATAGGTG GATTGATGAA ACCCCGCCAG TGGACCAGCC TTCCCGGTTT 
GGGAACAAAG CCTACAGAAC CTGGTATGCC AAACTTGATC AGGAAGCAGA AAACTTGGTG 
GCCACAGTGG TCCCCACCCA CCTGGCTGCT GCTGTGCCTG AAGTGGCAGT TTACCTGAAG 
GAGGCTGTGG GGAACTCCAC ACGAATTGAC TATGGCACAG GGCATGAGGC TGCCTTTGCT 
GCTTTCCTCT GTTGTCTCTG CAAGATTGGT GTACTCCGGG TGGACGACCA GGTGGCTATT 
GTCTTCAAGG TGTTTGATAG GTATCTTGAG GTTATGCGGA AGTTGCAGAA GACATACAGG 
ATGGAGCCTG CAGGCAGCCA GGGCGTATGG GGTCTGGATG ACTTCCAGTT CCTGCCCTTC 
ATCTGGGGCA GCTCACAGCT CATAGATGAA GACTGGCCCC TTTGCGGAAC ACTCCAACCA 
GCTGTGGAAC ATCAGTGCTG TCCCCTCCTG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497671.1
CDS	185..940
Translation	MAEGERQPPPDSSEETPPTTQNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFDYKVSEAIEKLVALLDTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDQEAENLVATVVPTHLAAAVPEVAVYLKEAVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQVAIVFKVFDRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDEDWPLCGTLQPAVEHQCCPLLV

Target ORF information:

RefSeq Version	XM_006497608.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497608.2

ORF Insert Sequence:

ATGGCCGAGG GCGAGCGGCA GCCGCCGCCA GATTCTTCAG AAGAGACCCC TCCAACTACT 
CAGAACTTTA TCATTCCAAA AAAGGAGATC CACACAGTTC CAGATATGGG CAAATGGAAG 
CGCTCTCAGG CATATGCTGA CTACATTGGC TTCATCCTTA CCCTCAATGA AGGTGTGAAG 
GGGAAGAAGC TGACCTTCGA CTACAAAGTC TCTGAGGCCA TCGAGAAGCT GGTGGCACTT 
CTTGATACGC TGGATAGGTG GATTGATGAA ACCCCGCCAG TGGACCAGCC TTCCCGGTTT 
GGGAACAAAG CCTACAGAAC CTGGTATGCC AAACTTGATC AGGAAGCAGA AAACTTGGTG 
GCCACAGTGG TCCCCACCCA CCTGGCTGCT GCTGTGCCTG AAGTGGCAGT TTACCTGAAG 
GAGGCTGTGG GGAACTCCAC ACGAATTGAC TATGGCACAG GGCATGAGGC TGCCTTTGCT 
GCTTTCCTCT GTTGTCTCTG CAAGATTGGT GTACTCCGGG TGGACGACCA GGTGGCTATT 
GTCTTCAAGG TGTTTGATAG GTATCTTGAG GTTATGCGGA AGTTGCAGAA GACATACAGG 
ATGGAGCCTG CAGGCAGCCA GGGCGTATGG GGTCTGGATG ACTTCCAGTT CCTGCCCTTC 
ATCTGGGGCA GCTCACAGCT CATAGATGAA GACTGGCCCC TTTGCGGAAC ACTCCAACCA 
GCTGTGGAAC ATCAGTGCTG TCCCCTCCTG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptpa ( NM_001362733.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349662.1 Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu84339
Clone ID Related Accession (Same CDS sequence)	NM_001362733.1
Accession Version	NM_001362733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein phosphatase 2A activator isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954299.13. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG GCGAGCGGCA GCCGCCGCCA GATTCTTCAG AAGAGACCCC TCCAACTACT 
CAGAACTTTA TCATTCCAAA AAAGGAGATC CACACAGTTC CAGATATGGG CAAATGGAAG 
CGCTCTCAGG CATATGCTGA CTACATTGGC TTCATCCTTA CCCTCAATGA AGGTGTGAAG 
GGGAAGAAGC TGACCTTCGA CTACAAAGTC TCTGAGGCCA TCGAGAAGCT GGTGGCACTT 
CTTGATACGC TGGATAGGTG GATTGATGAA ACCCCGCCAG TGGACCAGCC TTCCCGGTTT 
GGGAACAAAG CCTACAGAAC CTGGTATGCC AAACTTGATC AGGAAGCAGA AAACTTGGTG 
GCCACAGTGG TCCCCACCCA CCTGGCTGCT GCTGTGCCTG AAGTGGCAGT TTACCTGAAG 
GAGGCTGTGG GGAACTCCAC ACGAATTGAC TATGGCACAG GGCATGAGGC TGCCTTTGCT 
GCTTTCCTCT GTTGTCTCTG CAAGATTGGT GTACTCCGGG TGGACGACCA GGTGGCTATT 
GTCTTCAAGG TGTTTGATAG GTATCTTGAG GTTATGCGGA AGTTGCAGAA GACATACAGG 
ATGGAGCCTG CAGGCAGCCA GGGCGTATGG GGTCTGGATG ACTTCCAGTT CCTGCCCTTC 
ATCTGGGGCA GCTCACAGCT CATAGGTACT AACCACCCCC ACCTGGAGCC CAGACATTTC 
GTGGATGAGA AGGCGGTGAG CGAGAACCAC AAGGACTACA TGTTTCTCCA GTGCATCCTG 
TTCATCACTG AGATGAAGAC TGGCCCCTTT GCGGAACACT CCAACCAGCT GTGGAACATC 
AGTGCTGTCC CCTCCTGGTC TAAAGTGAAC CAGGGCCTCA TTCGAATGTA TAAGGCAGAG 
TGCCTGGAGA AGTTCCCTGT GATCCAGCAC TTCAAGTTCG GGAGCCTGCT GCCCATCCAT 
CCCGTCACAT CAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349662.1
CDS	253..1230
Translation	MAEGERQPPPDSSEETPPTTQNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFDYKVSEAIEKLVALLDTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDQEAENLVATVVPTHLAAAVPEVAVYLKEAVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQVAIVFKVFDRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIGTNHPHLEPRHFVDEKAVSENHKDYMFLQCILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG

Target ORF information:

RefSeq Version	NM_001362733.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 2 protein activator (Ptpa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362733.1

ORF Insert Sequence:

ATGGCCGAGG GCGAGCGGCA GCCGCCGCCA GATTCTTCAG AAGAGACCCC TCCAACTACT 
CAGAACTTTA TCATTCCAAA AAAGGAGATC CACACAGTTC CAGATATGGG CAAATGGAAG 
CGCTCTCAGG CATATGCTGA CTACATTGGC TTCATCCTTA CCCTCAATGA AGGTGTGAAG 
GGGAAGAAGC TGACCTTCGA CTACAAAGTC TCTGAGGCCA TCGAGAAGCT GGTGGCACTT 
CTTGATACGC TGGATAGGTG GATTGATGAA ACCCCGCCAG TGGACCAGCC TTCCCGGTTT 
GGGAACAAAG CCTACAGAAC CTGGTATGCC AAACTTGATC AGGAAGCAGA AAACTTGGTG 
GCCACAGTGG TCCCCACCCA CCTGGCTGCT GCTGTGCCTG AAGTGGCAGT TTACCTGAAG 
GAGGCTGTGG GGAACTCCAC ACGAATTGAC TATGGCACAG GGCATGAGGC TGCCTTTGCT 
GCTTTCCTCT GTTGTCTCTG CAAGATTGGT GTACTCCGGG TGGACGACCA GGTGGCTATT 
GTCTTCAAGG TGTTTGATAG GTATCTTGAG GTTATGCGGA AGTTGCAGAA GACATACAGG 
ATGGAGCCTG CAGGCAGCCA GGGCGTATGG GGTCTGGATG ACTTCCAGTT CCTGCCCTTC 
ATCTGGGGCA GCTCACAGCT CATAGGTACT AACCACCCCC ACCTGGAGCC CAGACATTTC 
GTGGATGAGA AGGCGGTGAG CGAGAACCAC AAGGACTACA TGTTTCTCCA GTGCATCCTG 
TTCATCACTG AGATGAAGAC TGGCCCCTTT GCGGAACACT CCAACCAGCT GTGGAACATC 
AGTGCTGTCC CCTCCTGGTC TAAAGTGAAC CAGGGCCTCA TTCGAATGTA TAAGGCAGAG 
TGCCTGGAGA AGTTCCCTGT GATCCAGCAC TTCAAGTTCG GGAGCCTGCT GCCCATCCAT 
CCCGTCACAT CAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PP2A Inactivation Mediated by
Cancer research77(24)6825-6837(2017 Dec)
Sents W,Meeusen B,Kalev P,Radaelli E,Sagaert X,Miermans E,Haesen D,Lambrecht C,Dewerchin M,Carmeliet P,Westermarck J,Sablina A,Janssens V

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

A ternary complex comprising FAK, PTP? and IP3 receptor 1 functionally engages focal adhesions and the endoplasmic reticulum to mediate IL-1-induced Ca2+ signalling in fibroblasts.
The Biochemical journal473(4)397-410(2016 Feb)
Wang Q,Wang Y,Downey GP,Plotnikov S,McCulloch CA

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Developmental biology406(1)92-103(2015 Oct)
Lewandowski JP,Du F,Zhang S,Powell MB,Falkenstein KN,Ji H,Vokes SA

Phosphorylation status of nuclear ribosomal protein S3 is reciprocally regulated by protein kinase C{delta} and protein phosphatase 2A.
The Journal of biological chemistry284(32)21201-8(2009 Aug)
Kim TS,Kim HD,Shin HS,Kim J

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Ptpa cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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