Chrna3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrna3
Entrez Gene ID	110834
Full Name	cholinergic receptor, nicotinic, alpha polypeptide 3
Synonyms	(a)3,A730007P14Rik,Acra-3,Acra3
General protein information	Preferred Names cholinergic receptor, nicotinic, alpha polypeptide 3 Names neuronal acetylcholine receptor subunit alpha-3 alpha 3 neuronal nicotinic acetylcholine receptor, alpha 3 subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 B

mRNA and Protein(s)

mRNA	Protein	Name
NM_145129.2	NP_660111.2	neuronal acetylcholine receptor subunit alpha-3 precursor
NM_145129.3	NP_660111.3	neuronal acetylcholine receptor subunit alpha-3 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHRNA3	NP_000734.2
Pan troglodytes (chimpanzee)	CHRNA3	XP_003952761.1
Drosophila melanogaster (fruit fly)	nAcRalpha-80B	NP_001097669.2
Canis lupus familiaris (dog)	CHRNA3	XP_539232.4
Bos taurus (cattle)	CHRNA3	NP_777144.1
Gallus gallus (chicken)	CHRNA3	NP_989747.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000138	XP_566274.3
Xenopus tropicalis (tropical clawed frog)	LOC100498049	XP_002934623.2
Mus musculus (house mouse)	Chrna3	NP_660111.2
Rattus norvegicus (Norway rat)	Chrna3	NP_434692.2
Danio rerio (zebrafish)	chrna3	XP_001921314.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional and Molecular Characterization of Mechanoinsensitive "Silent" Nociceptors.
Prato V, Taberner FJ, Hockley JRF, Callejo G, Arcourt A, Tazir B, Hammer L, Schad P, Heppenstall PA, Smith ES, Lechner SG
Cell reports21(11)3102-3115(2017 Dec)

Regulation of Tlx3 by Pax6 is required for the restricted expression of Chrn?3 in Cerebellar Granule Neuron progenitors during development.
Divya TS, Lalitha S, Parvathy S, Subashini C, Sanalkumar R, Dhanesh SB, Rasheed VA, Divya MS, Tole S, James J
Scientific reports630337(2016 Jul)

Changes in Nicotinic Neurotransmission during Enteric Nervous System Development.
Foong JP, Hirst CS, Hao MM, McKeown SJ, Boesmans W, Young HM, Bornstein JC, Vanden Berghe P
The Journal of neuroscience : the official journal of the Society for Neuroscience35(18)7106-15(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

neuroinflammation is sufficient to provoke the decrease of a7 and a4b2 nAChRs, Ab42 accumulation and memory impairment in mice and a7(1-208) nAChR-specific antibodies can cause inflammation of the brain with symptoms typical for Alzheimer disease
Title: ?7 Nicotinic acetylcholine receptor-specific antibody induces inflammation and amyloid ?42 accumulation in the mouse brain to impair memory.

The alpha3beta4* nicotinic ACh receptor subtype mediates physical dependence to morphine
Title: The ?3?4* nicotinic ACh receptor subtype mediates physical dependence to morphine: mouse and human studies.

The present findings suggest that its thymic production and/or release are under cholinergic control involving nAChR containing the alpha3-subunit.
Title: Terminally differentiated epithelial cells of the thymic medulla and skin express nicotinic acetylcholine receptor subunit ? 3.

Downregulation of nAChR subunit and PSD-93 expression after cavernous nerve injury, or even manipulation, could interrupt synaptic transmission within the MPG and thus contribute to the loss of neural control of urogenital organs after pelvic surgeries.
Title: Decrease in neuronal nicotinic acetylcholine receptor subunit and PSD-93 transcript levels in the male mouse MPG after cavernous nerve injury or explant culture.

findings suggest an important role for the a3b4* nAChR subtype in nicotine reward and physical aspects of the nicotine withdrawal syndrome.
Title: The ?3?4* nicotinic acetylcholine receptor subtype mediates nicotine reward and physical nicotine withdrawal signs independently of the ?5 subunit in the mouse.

The following Chrna3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrna3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11314	NM_145129.2 Latest version!	Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OMu84761	NM_145129.3 Latest version!	Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrna3 ( NM_145129.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_660111.2 Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3), mRNA.

Price & Availability↑

CloneID	OMu11314
Clone ID Related Accession (Same CDS sequence)	NM_145129.2
Accession Version	NM_145129.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Mus musculus(house mouse)
Product	neuronal acetylcholine receptor subunit alpha-3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK080415.1, AK051730.1 and AK041217.1. On Jul 13, 2005 this sequence version replaced NM_145129.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK080415.1, AK051730.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849377, SAMN00849379 [ECO:0000350] ##Evidence-Data-END##

ATGCGGTCTT CAGACATGGG TGTTGTGCTG CCCCCGCCGC CGCTGTCCAT GCTGATGCTG 
GTGCTGATGC TGCTGCCAGT GGCCAGCGCC TCGGAAGCTG AGCACCGCCT GTTCCAGTAC 
CTGTTTGAAG ATTACAACGA GATCATCCGG CCGGTGGCTA ACGTGTCCCA TCCTGTCATC 
ATCCAGTTTG AGGTGTCTAT GTCTCAGCTG GTGAAGGTGG ATGAAGTAAA CCAGATCATG 
GAAACCAACC TGTGGCTGAA GCAAATCTGG AATGACTACA AGCTGAAATG GAAACCCTCT 
GACTACCAAG GGGTGGAGTT CATGCGAGTC CCTGCAGAGA AGATCTGGAA ACCAGACATC 
GTGCTTTACA ACAACGCCGA TGGGGATTTC CAAGTGGATG ACAAAACCAA AGCTCTACTC 
AAGTACACAG GAGAAGTGAC TTGGATCCCT CCGGCCATCT TTAAGAGCTC ATGCAAAATC 
GATGTGACCT ACTTCCCGTT TGACTACCAA AACTGCACCA TGAAGTTCGG CTCCTGGTCC 
TACGACAAGG CAAAGATCGA CCTGGTCCTC ATTGGCTCCT CCATGAACCT CAAGGACTAT 
TGGGAAAGTG GCGAGTGGGC CATCATTAAA GCCCCGGGCT ACAAACATGA AATCAAGTAC 
AACTGCTGTG AGGAGATCTA CCAAGACATC ACGTACTCGC TATACATTCG CCGCCTGCCG 
CTGTTCTACA CCATCAACCT CATCATTCCG TGCCTGCTCA TCTCCTTCCT CACTGTGCTC 
GTCTTCTACC TGCCCTCCGA CTGTGGGGAG AAGGTGACGC TCTGCATCTC CGTGCTCCTC 
TCCCTGACGG TCTTTCTCCT CGTGATCACC GAGACCATCC CTTCCACCTC ACTGGTCATC 
CCCTTGATCG GGGAGTACCT CCTCTTCACT ATGATTTTTG TCACCTTGTC CATCGTCATC 
ACAGTCTTTG TGCTCAACGT GCACTACAGA ACTCCGACCA CACACACGAT GCCCACTTGG 
GTCAAGGCTG TGTTTTTGAA CCTTCTCCCC AGGGTCATGT TTATGACTAG GCCAACCAGC 
ACCGAGGAAG ACGCCCCAAA GACGAGGAAC TTCTACGGTG CTGAGCTCTC AAACCTGAAC 
TGCTTCAGCC GTGCAGACTC CAAAAGCTGC AAGGAAGGCT ACCCCTGCCA AGATGGGACA 
TGTGGCTATT GCCACCACCG TAGGGTAAAA ATCTCAAATT TCAGTGCCAA CCTCACAAGA 
AGCTCCAGTT CTGAGTCTGT TGATGCTGTG TTGTCCCTGT CTGCTCTGTC ACCAGAAATC 
AAAGAAGCCA TCCAAAGTGT GAAGTATATT GCTGAAAATA TGAAAGCACA GAATGTAGCC 
AAAGAGATTC AAGATGATTG GAAGTATGTT GCCATGGTGA TTGATCGCAT TTTTCTCTGG 
GTTTTCATCC TGGTGTGCAT TTTAGGAACT GCAGGATTAT TTCTGCAACC CTTGATGGCC 
AGAGATGACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660111.2
CDS	151..1665
Translation	MRSSDMGVVLPPPPLSMLMLVLMLLPVASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRPTSTEEDAPKTRNFYGAELSNLNCFSRADSKSCKEGYPCQDGTCGYCHHRRVKISNFSANLTRSSSSESVDAVLSLSALSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLMARDDT

Target ORF information:

RefSeq Version	NM_145129.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145129.2

ORF Insert Sequence:

ATGCGGTCTT CAGACATGGG TGTTGTGCTG CCCCCGCCGC CGCTGTCCAT GCTGATGCTG 
GTGCTGATGC TGCTGCCAGT GGCCAGCGCC TCGGAAGCTG AGCACCGCCT GTTCCAGTAC 
CTGTTTGAAG ATTACAACGA GATCATCCGG CCGGTGGCTA ACGTGTCCCA TCCTGTCATC 
ATCCAGTTTG AGGTGTCTAT GTCTCAGCTG GTGAAGGTGG ATGAAGTAAA CCAGATCATG 
GAAACCAACC TGTGGCTGAA GCAAATCTGG AATGACTACA AGCTGAAATG GAAACCCTCT 
GACTACCAAG GGGTGGAGTT CATGCGAGTC CCTGCAGAGA AGATCTGGAA ACCAGACATC 
GTGCTTTACA ACAACGCCGA TGGGGATTTC CAAGTGGATG ACAAAACCAA AGCTCTACTC 
AAGTACACAG GAGAAGTGAC TTGGATCCCT CCGGCCATCT TTAAGAGCTC ATGCAAAATC 
GATGTGACCT ACTTCCCGTT TGACTACCAA AACTGCACCA TGAAGTTCGG CTCCTGGTCC 
TACGACAAGG CAAAGATCGA CCTGGTCCTC ATTGGCTCCT CCATGAACCT CAAGGACTAT 
TGGGAAAGTG GCGAGTGGGC CATCATTAAA GCCCCGGGCT ACAAACATGA AATCAAGTAC 
AACTGCTGTG AGGAGATCTA CCAAGACATC ACGTACTCGC TATACATTCG CCGCCTGCCG 
CTGTTCTACA CCATCAACCT CATCATTCCG TGCCTGCTCA TCTCCTTCCT CACTGTGCTC 
GTCTTCTACC TGCCCTCCGA CTGTGGGGAG AAGGTGACGC TCTGCATCTC CGTGCTCCTC 
TCCCTGACGG TCTTTCTCCT CGTGATCACC GAGACCATCC CTTCCACCTC ACTGGTCATC 
CCCTTGATCG GGGAGTACCT CCTCTTCACT ATGATTTTTG TCACCTTGTC CATCGTCATC 
ACAGTCTTTG TGCTCAACGT GCACTACAGA ACTCCGACCA CACACACGAT GCCCACTTGG 
GTCAAGGCTG TGTTTTTGAA CCTTCTCCCC AGGGTCATGT TTATGACTAG GCCAACCAGC 
ACCGAGGAAG ACGCCCCAAA GACGAGGAAC TTCTACGGTG CTGAGCTCTC AAACCTGAAC 
TGCTTCAGCC GTGCAGACTC CAAAAGCTGC AAGGAAGGCT ACCCCTGCCA AGATGGGACA 
TGTGGCTATT GCCACCACCG TAGGGTAAAA ATCTCAAATT TCAGTGCCAA CCTCACAAGA 
AGCTCCAGTT CTGAGTCTGT TGATGCTGTG TTGTCCCTGT CTGCTCTGTC ACCAGAAATC 
AAAGAAGCCA TCCAAAGTGT GAAGTATATT GCTGAAAATA TGAAAGCACA GAATGTAGCC 
AAAGAGATTC AAGATGATTG GAAGTATGTT GCCATGGTGA TTGATCGCAT TTTTCTCTGG 
GTTTTCATCC TGGTGTGCAT TTTAGGAACT GCAGGATTAT TTCTGCAACC CTTGATGGCC 
AGAGATGACA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrna3 ( NM_145129.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_660111.3 Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3), mRNA.

Price & Availability↑

CloneID	OMu84761
Clone ID Related Accession (Same CDS sequence)	NM_145129.3
Accession Version	NM_145129.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	neuronal acetylcholine receptor subunit alpha-3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC156832.2 and AC161264.2. On Mar 21, 2019 this sequence version replaced NM_145129.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK051730.1, AK080415.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849377, SAMN00849379 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGGGTGTTG TGCTGCCCCC GCCGCCGCTG TCCATGCTGA TGCTGGTGCT GATGCTGCTG 
CCAGTGGCCA GCGCCTCGGA AGCTGAGCAC CGCCTGTTCC AGTACCTGTT TGAAGATTAC 
AACGAGATCA TCCGGCCGGT GGCTAACGTG TCCCATCCTG TCATCATCCA GTTTGAGGTG 
TCTATGTCTC AGCTGGTGAA GGTGGATGAA GTAAACCAGA TCATGGAAAC CAACCTGTGG 
CTGAAGCAAA TCTGGAATGA CTACAAGCTG AAATGGAAAC CCTCTGACTA CCAAGGGGTG 
GAGTTCATGC GAGTCCCTGC AGAGAAGATC TGGAAACCAG ACATCGTGCT TTACAACAAC 
GCCGATGGGG ATTTCCAAGT GGATGACAAA ACCAAAGCTC TACTCAAGTA CACAGGAGAA 
GTGACTTGGA TCCCTCCGGC CATCTTTAAG AGCTCATGCA AAATCGATGT GACCTACTTC 
CCGTTTGACT ACCAAAACTG CACCATGAAG TTCGGCTCCT GGTCCTACGA CAAGGCAAAG 
ATCGACCTGG TCCTCATTGG CTCCTCCATG AACCTCAAGG ACTATTGGGA AAGTGGCGAG 
TGGGCCATCA TTAAAGCCCC GGGCTACAAA CATGAAATCA AGTACAACTG CTGTGAGGAG 
ATCTACCAAG ACATCACGTA CTCGCTATAC ATTCGCCGCC TGCCGCTGTT CTACACCATC 
AACCTCATCA TTCCGTGCCT GCTCATCTCC TTCCTCACTG TGCTCGTCTT CTACCTGCCC 
TCCGACTGTG GGGAGAAGGT GACGCTCTGC ATCTCCGTGC TCCTCTCCCT GACGGTCTTT 
CTCCTCGTGA TCACCGAGAC CATCCCTTCC ACCTCACTGG TCATCCCCTT GATCGGGGAG 
TACCTCCTCT TCACTATGAT TTTTGTCACC TTGTCCATCG TCATCACAGT CTTTGTGCTC 
AACGTGCACT ACAGAACTCC GACCACACAC ACGATGCCCA CTTGGGTCAA GGCTGTGTTT 
TTGAACCTTC TCCCCAGGGT CATGTTTATG ACTAGGCCAA CCAGCACCGA GGAAGACGCC 
CCAAAGACGA GGAACTTCTA CGGTGCTGAG CTCTCAAACC TGAACTGCTT CAGCCGTGCA 
GACTCCAAAA GCTGCAAGGA AGGCTACCCC TGCCAAGATG GGACATGTGG CTATTGCCAC 
CACCGTAGGG TAAAAATCTC AAATTTCAGT GCCAACCTCA CAAGAAGCTC CAGTTCTGAG 
TCTGTTGATG CTGTGTTGTC CCTGTCTGCT CTGTCACCAG AAATCAAAGA AGCCATCCAA 
AGTGTGAAGT ATATTGCTGA AAATATGAAA GCACAGAATG TAGCCAAAGA GATTCAAGAT 
GATTGGAAGT ATGTTGCCAT GGTGATTGAT CGCATTTTTC TCTGGGTTTT CATCCTGGTG 
TGCATTTTAG GAACTGCAGG ATTATTTCTG CAACCCTTGA TGGCCAGAGA TGACACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660111.3
CDS	169..1668
Translation	MGVVLPPPPLSMLMLVLMLLPVASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRPTSTEEDAPKTRNFYGAELSNLNCFSRADSKSCKEGYPCQDGTCGYCHHRRVKISNFSANLTRSSSSESVDAVLSLSALSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLMARDDT

Target ORF information:

RefSeq Version	NM_145129.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 3 (Chrna3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145129.3

ORF Insert Sequence:

ATGGGTGTTG TGCTGCCCCC GCCGCCGCTG TCCATGCTGA TGCTGGTGCT GATGCTGCTG 
CCAGTGGCCA GCGCCTCGGA AGCTGAGCAC CGCCTGTTCC AGTACCTGTT TGAAGATTAC 
AACGAGATCA TCCGGCCGGT GGCTAACGTG TCCCATCCTG TCATCATCCA GTTTGAGGTG 
TCTATGTCTC AGCTGGTGAA GGTGGATGAA GTAAACCAGA TCATGGAAAC CAACCTGTGG 
CTGAAGCAAA TCTGGAATGA CTACAAGCTG AAATGGAAAC CCTCTGACTA CCAAGGGGTG 
GAGTTCATGC GAGTCCCTGC AGAGAAGATC TGGAAACCAG ACATCGTGCT TTACAACAAC 
GCCGATGGGG ATTTCCAAGT GGATGACAAA ACCAAAGCTC TACTCAAGTA CACAGGAGAA 
GTGACTTGGA TCCCTCCGGC CATCTTTAAG AGCTCATGCA AAATCGATGT GACCTACTTC 
CCGTTTGACT ACCAAAACTG CACCATGAAG TTCGGCTCCT GGTCCTACGA CAAGGCAAAG 
ATCGACCTGG TCCTCATTGG CTCCTCCATG AACCTCAAGG ACTATTGGGA AAGTGGCGAG 
TGGGCCATCA TTAAAGCCCC GGGCTACAAA CATGAAATCA AGTACAACTG CTGTGAGGAG 
ATCTACCAAG ACATCACGTA CTCGCTATAC ATTCGCCGCC TGCCGCTGTT CTACACCATC 
AACCTCATCA TTCCGTGCCT GCTCATCTCC TTCCTCACTG TGCTCGTCTT CTACCTGCCC 
TCCGACTGTG GGGAGAAGGT GACGCTCTGC ATCTCCGTGC TCCTCTCCCT GACGGTCTTT 
CTCCTCGTGA TCACCGAGAC CATCCCTTCC ACCTCACTGG TCATCCCCTT GATCGGGGAG 
TACCTCCTCT TCACTATGAT TTTTGTCACC TTGTCCATCG TCATCACAGT CTTTGTGCTC 
AACGTGCACT ACAGAACTCC GACCACACAC ACGATGCCCA CTTGGGTCAA GGCTGTGTTT 
TTGAACCTTC TCCCCAGGGT CATGTTTATG ACTAGGCCAA CCAGCACCGA GGAAGACGCC 
CCAAAGACGA GGAACTTCTA CGGTGCTGAG CTCTCAAACC TGAACTGCTT CAGCCGTGCA 
GACTCCAAAA GCTGCAAGGA AGGCTACCCC TGCCAAGATG GGACATGTGG CTATTGCCAC 
CACCGTAGGG TAAAAATCTC AAATTTCAGT GCCAACCTCA CAAGAAGCTC CAGTTCTGAG 
TCTGTTGATG CTGTGTTGTC CCTGTCTGCT CTGTCACCAG AAATCAAAGA AGCCATCCAA 
AGTGTGAAGT ATATTGCTGA AAATATGAAA GCACAGAATG TAGCCAAAGA GATTCAAGAT 
GATTGGAAGT ATGTTGCCAT GGTGATTGAT CGCATTTTTC TCTGGGTTTT CATCCTGGTG 
TGCATTTTAG GAACTGCAGG ATTATTTCTG CAACCCTTGA TGGCCAGAGA TGACACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional and Molecular Characterization of Mechanoinsensitive "Silent" Nociceptors.
Cell reports21(11)3102-3115(2017 Dec)
Prato V,Taberner FJ,Hockley JRF,Callejo G,Arcourt A,Tazir B,Hammer L,Schad P,Heppenstall PA,Smith ES,Lechner SG

Regulation of Tlx3 by Pax6 is required for the restricted expression of Chrn?3 in Cerebellar Granule Neuron progenitors during development.
Scientific reports630337(2016 Jul)
Divya TS,Lalitha S,Parvathy S,Subashini C,Sanalkumar R,Dhanesh SB,Rasheed VA,Divya MS,Tole S,James J

Changes in Nicotinic Neurotransmission during Enteric Nervous System Development.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(18)7106-15(2015 May)
Foong JP,Hirst CS,Hao MM,McKeown SJ,Boesmans W,Young HM,Bornstein JC,Vanden Berghe P

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Chrna3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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