Srsf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Srsf1
Entrez Gene ID	110809
Full Name	serine/arginine-rich splicing factor 1
Synonyms	1110054N12Rik,5730507C05Rik,6330415C05Rik,AI482334,AW491331,Asf,Sf2,Sfrs1
General protein information	Preferred Names serine/arginine-rich splicing factor 1 Names serine/arginine-rich splicing factor 1 ASF/SF2 pre-mRNA-splicing factor SRp30a splicing factor, arginine/serine-rich 1 (ASF/SF2)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 C\|11 52.4 cM
Summary	The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001078167.2	NP_001071635.1	serine/arginine-rich splicing factor 1 isoform 2
NM_173374.4	NP_775550.2	serine/arginine-rich splicing factor 1 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SRSF1	NP_001182476.1
Gallus gallus (chicken)	SRSF1	NP_001107213.1
Xenopus tropicalis (tropical clawed frog)	srsf1	NP_001006919.1
Homo sapiens (human)	SRSF1	NP_008855.1
Danio rerio (zebrafish)	srsf1a	NP_998180.1
Drosophila melanogaster (fruit fly)	SF2	NP_652611.1
Mus musculus (house mouse)	Srsf1	NP_775550.2
Arabidopsis thaliana (thale cress)	SR1	NP_850934.1
Canis lupus familiaris (dog)	SRSF1	XP_005624772.1
Bos taurus (cattle)	SRSF1	NP_001069862.1
	SRSF1	XP_001103473.2
Rattus norvegicus (Norway rat)	Srsf1	NP_001103022.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010496	XP_314469.4
Caenorhabditis elegans (roundworm)	rsp-3	NP_499649.2
Arabidopsis thaliana (thale cress)	AT4G02430	NP_567235.3

Gene Ontology
Bibliography

Related articles in PubMed

Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase.
Sunatani Y, Kamdar RP, Sharma MK, Matsui T, Sakasai R, Hashimoto M, Ishigaki Y, Matsumoto Y, Iwabuchi K
Experimental cell research362(2)450-460(2018 Jan)

A SRSF1 self-binding mechanism restrains Mir505-3p from inhibiting proliferation of neural tumor cell lines.
Yang K, Tong L, Li K, Zhou Y, Xiao J
Anti-cancer drugs29(1)40-49(2018 Jan)

Conserved expression of ultra-conserved noncoding RNA in mammalian nervous system.
Zhou J, Wang R, Zhang J, Zhu L, Liu W, Lu S, Chen P, Li H, Yin B, Yuan J, Qiang B, Shu P, Peng X
Biochimica et biophysica acta1860(12)1159-1168(2017 Dec)

SRSF1 Prevents DNA Damage and Promotes Tumorigenesis through Regulation of DBF4B Pre-mRNA Splicing.
Chen L, Luo C, Shen L, Liu Y, Wang Q, Zhang C, Guo R, Zhang Y, Xie Z, Wei N, Wu W, Han J, Feng Y
Cell reports21(12)3406-3413(2017 Dec)

SRSF1 promotes vascular smooth muscle cell proliferation through a ?133p53/EGR1/KLF5 pathway.
Xie N, Chen M, Dai R, Zhang Y, Zhao H, Song Z, Zhang L, Li Z, Feng Y, Gao H, Wang L, Zhang T, Xiao RP, Wu J, Cao CM
Nature communications816016(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RRP1B suppresses metastatic progression by altering the transcriptome through its interaction with splicing regulators such as SRSF1
Title: RRP1B is a metastasis modifier that regulates the expression of alternative mRNA isoforms through interactions with SRSF1.

The expression levels of three splicing factors, ESRP1, PTB and SF2/ASF, are significantly altered during cardiac hypertrophy in mice.
Title: Pressure-overload cardiac hypertrophy is associated with distinct alternative splicing due to altered expression of splicing factors.

Deletion of RRM1 eliminated the splicing activity of SRSF1 and thus cellular transformation.
Title: RRM1 domain of the splicing oncoprotein SRSF1 is required for MEK1-MAPK-ERK activation and cellular transformation.

Specific effects on regulated splicing by SR proteins SRSF1 and SRSF2 depends on a complex set of relationships with multiple other SR proteins in mammalian genomes.
Title: Genome-wide analysis reveals SR protein cooperation and competition in regulated splicing.

Treatment with IL-17 prolongs the half-life of chemokine CXCL1 mRNA via the adaptor TRAF5 and the splicing-regulatory factor SF2 (ASF).
Title: Treatment with IL-17 prolongs the half-life of chemokine CXCL1 mRNA via the adaptor TRAF5 and the splicing-regulatory factor SF2 (ASF).

The following Srsf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srsf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19637	NM_173374.4 Latest version!	Mus musculus serine and arginine-rich splicing factor 1 (Srsf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
OMu19330	NM_001078167.2 Latest version!	Mus musculus serine and arginine-rich splicing factor 1 (Srsf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Srsf1 ( NM_173374.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_775550.2 Mus musculus serine and arginine-rich splicing factor 1 (Srsf1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu19637
Clone ID Related Accession (Same CDS sequence)	NM_173374.4
Accession Version	NM_173374.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	serine/arginine-rich splicing factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CF739274.1, AK053674.1 and AL593853.11. On Sep 28, 2012 this sequence version replaced NM_173374.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK053674.1, BC058627.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGGAG GTGGTGTGAT CCGTGGCCCG GCGGGGAACA ACGACTGCCG CATCTACGTG 
GGTAACCTAC CTCCGGATAT CCGAACCAAG GACATCGAGG ACGTGTTTTA CAAATACGGC 
GCCATCCGCG ACATCGACCT GAAGAACCGC CGCGGGGGAC CGCCCTTCGC CTTCGTTGAG 
TTCGAGGACC CGCGAGACGC GGAAGATGCG GTGTACGGTC GCGACGGCTA CGACTACGAC 
GGCTACCGGC TGCGGGTAGA GTTTCCCCGA AGCGGCCGCG GGACCGGCCG AGGCGGCGGC 
GGGGGTGGAG GCGGCGGCGC CCCGAGAGGC CGCTATGGCC CGCCGTCCAG GCGGTCCGAG 
AACAGAGTGG TTGTCTCTGG ACTGCCTCCG AGTGGAAGCT GGCAGGACTT AAAGGATCAC 
ATGCGTGAGG CAGGTGATGT ATGTTACGCT GATGTTTACC GAGATGGCAC TGGTGTCGTG 
GAGTTTGTAC GGAAAGAAGA TATGACGTAT GCAGTTCGAA AACTGGATAA CACTAAGTTT 
AGATCTCACG AGGGAGAAAC TGCCTACATC CGGGTTAAAG TTGATGGGCC CAGAAGTCCA 
AGTTATGGAA GATCTCGATC TCGAAGCCGT AGTCGTAGCA GAAGCCGTAG CAGAAGCAAC 
AGCAGGAGTC GCAGTTACTC CCCAAGGAGA AGCAGAGGAT CACCACGCTA TTCTCCCCGT 
CATAGCAGAT CTCGCTCTCG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775550.2
CDS	467..1213
Translation	MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSRSNSRSRSYSPRRSRGSPRYSPRHSRSRSRT

Target ORF information:

RefSeq Version	NM_173374.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine and arginine-rich splicing factor 1 (Srsf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173374.4

ORF Insert Sequence:

ATGTCGGGAG GTGGTGTGAT CCGTGGCCCG GCGGGGAACA ACGACTGCCG CATCTACGTG 
GGTAACCTAC CTCCGGATAT CCGAACCAAG GACATCGAGG ACGTGTTTTA CAAATACGGC 
GCCATCCGCG ACATCGACCT GAAGAACCGC CGCGGGGGAC CGCCCTTCGC CTTCGTTGAG 
TTCGAGGACC CGCGAGACGC GGAAGATGCG GTGTACGGTC GCGACGGCTA CGACTACGAC 
GGCTACCGGC TGCGGGTAGA GTTTCCCCGA AGCGGCCGCG GGACCGGCCG AGGCGGCGGC 
GGGGGTGGAG GCGGCGGCGC CCCGAGAGGC CGCTATGGCC CGCCGTCCAG GCGGTCCGAG 
AACAGAGTGG TTGTCTCTGG ACTGCCTCCG AGTGGAAGCT GGCAGGACTT AAAGGATCAC 
ATGCGTGAGG CAGGTGATGT ATGTTACGCT GATGTTTACC GAGATGGCAC TGGTGTCGTG 
GAGTTTGTAC GGAAAGAAGA TATGACGTAT GCAGTTCGAA AACTGGATAA CACTAAGTTT 
AGATCTCACG AGGGAGAAAC TGCCTACATC CGGGTTAAAG TTGATGGGCC CAGAAGTCCA 
AGTTATGGAA GATCTCGATC TCGAAGCCGT AGTCGTAGCA GAAGCCGTAG CAGAAGCAAC 
AGCAGGAGTC GCAGTTACTC CCCAAGGAGA AGCAGAGGAT CACCACGCTA TTCTCCCCGT 
CATAGCAGAT CTCGCTCTCG TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srsf1 ( NM_001078167.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001071635.1 Mus musculus serine and arginine-rich splicing factor 1 (Srsf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu19330
Clone ID Related Accession (Same CDS sequence)	NM_001078167.2
Accession Version	NM_001078167.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-14
Organism	Mus musculus(house mouse)
Product	serine/arginine-rich splicing factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CF739274.1, AK078715.1, AK053674.1 and AL593853.11. On Sep 29, 2012 this sequence version replaced NM_001078167.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK078715.1, X66091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGGAG GTGGTGTGAT CCGTGGCCCG GCGGGGAACA ACGACTGCCG CATCTACGTG 
GGTAACCTAC CTCCGGATAT CCGAACCAAG GACATCGAGG ACGTGTTTTA CAAATACGGC 
GCCATCCGCG ACATCGACCT GAAGAACCGC CGCGGGGGAC CGCCCTTCGC CTTCGTTGAG 
TTCGAGGACC CGCGAGACGC GGAAGATGCG GTGTACGGTC GCGACGGCTA CGACTACGAC 
GGCTACCGGC TGCGGGTAGA GTTTCCCCGA AGCGGCCGCG GGACCGGCCG AGGCGGCGGC 
GGGGGTGGAG GCGGCGGCGC CCCGAGAGGC CGCTATGGCC CGCCGTCCAG GCGGTCCGAG 
AACAGAGTGG TTGTCTCTGG ACTGCCTCCG AGTGGAAGCT GGCAGGACTT AAAGGATCAC 
ATGCGTGAGG CAGGTGATGT ATGTTACGCT GATGTTTACC GAGATGGCAC TGGTGTCGTG 
GAGTTTGTAC GGAAAGAAGA TATGACGTAT GCAGTTCGAA AACTGGATAA CACTAAGTTT 
AGATCTCACG AGGTAGGTTA TACACTTATT CTTTTTTTTG GCCAGAATTG GATACAGTTT 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071635.1
CDS	467..1072
Translation	MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEVGYTLILFFGQNWIQFS

Target ORF information:

RefSeq Version	NM_001078167.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine and arginine-rich splicing factor 1 (Srsf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078167.2

ORF Insert Sequence:

ATGTCGGGAG GTGGTGTGAT CCGTGGCCCG GCGGGGAACA ACGACTGCCG CATCTACGTG 
GGTAACCTAC CTCCGGATAT CCGAACCAAG GACATCGAGG ACGTGTTTTA CAAATACGGC 
GCCATCCGCG ACATCGACCT GAAGAACCGC CGCGGGGGAC CGCCCTTCGC CTTCGTTGAG 
TTCGAGGACC CGCGAGACGC GGAAGATGCG GTGTACGGTC GCGACGGCTA CGACTACGAC 
GGCTACCGGC TGCGGGTAGA GTTTCCCCGA AGCGGCCGCG GGACCGGCCG AGGCGGCGGC 
GGGGGTGGAG GCGGCGGCGC CCCGAGAGGC CGCTATGGCC CGCCGTCCAG GCGGTCCGAG 
AACAGAGTGG TTGTCTCTGG ACTGCCTCCG AGTGGAAGCT GGCAGGACTT AAAGGATCAC 
ATGCGTGAGG CAGGTGATGT ATGTTACGCT GATGTTTACC GAGATGGCAC TGGTGTCGTG 
GAGTTTGTAC GGAAAGAAGA TATGACGTAT GCAGTTCGAA AACTGGATAA CACTAAGTTT 
AGATCTCACG AGGTAGGTTA TACACTTATT CTTTTTTTTG GCCAGAATTG GATACAGTTT 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase.
Experimental cell research362(2)450-460(2018 Jan)
Sunatani Y,Kamdar RP,Sharma MK,Matsui T,Sakasai R,Hashimoto M,Ishigaki Y,Matsumoto Y,Iwabuchi K

A SRSF1 self-binding mechanism restrains Mir505-3p from inhibiting proliferation of neural tumor cell lines.
Anti-cancer drugs29(1)40-49(2018 Jan)
Yang K,Tong L,Li K,Zhou Y,Xiao J

Conserved expression of ultra-conserved noncoding RNA in mammalian nervous system.
Biochimica et biophysica acta1860(12)1159-1168(2017 Dec)
Zhou J,Wang R,Zhang J,Zhu L,Liu W,Lu S,Chen P,Li H,Yin B,Yuan J,Qiang B,Shu P,Peng X

SRSF1 Prevents DNA Damage and Promotes Tumorigenesis through Regulation of DBF4B Pre-mRNA Splicing.
Cell reports21(12)3406-3413(2017 Dec)
Chen L,Luo C,Shen L,Liu Y,Wang Q,Zhang C,Guo R,Zhang Y,Xie Z,Wei N,Wu W,Han J,Feng Y

SRSF1 promotes vascular smooth muscle cell proliferation through a ?133p53/EGR1/KLF5 pathway.
Nature communications816016(2017 Aug)
Xie N,Chen M,Dai R,Zhang Y,Zhao H,Song Z,Zhang L,Li Z,Feng Y,Gao H,Wang L,Zhang T,Xiao RP,Wu J,Cao CM

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Srsf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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