Eef1akmt4-ece2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eef1akmt4-ece2
Entrez Gene ID	110599584
Full Name	Eef1akmt4-Ece2 readthrough
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 B1
Summary	This locus represents naturally occurring readthrough transcription between the adjacent genes eukaryotic translation elongation factor 1 alpha lysine specific methyltransferase 4 (GeneID:110599566) and endothelin converting enzyme 2 (GeneID:107522). The readthrough transcript representing this gene encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_177940.1	NP_808809.1	Eef1akmt4-Ece2 readthrough isoform b
NM_177941.1	NP_808810.1	Eef1akmt4-Ece2 readthrough isoform a

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eef1akmt4-ece2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eef1akmt4-ece2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07049	NM_177940.1 Latest version!	Mus musculus Eef1akmt4-Ece2 readthrough (Eef1akmt4-ece2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu07050	NM_177941.1 Latest version!	Mus musculus Eef1akmt4-Ece2 readthrough (Eef1akmt4-ece2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eef1akmt4-ece2 ( NM_177940.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808809.1 Mus musculus Eef1akmt4-Ece2 readthrough (Eef1akmt4-ece2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07049
Clone ID Related Accession (Same CDS sequence)	NM_177940.1
Accession Version	NM_177940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	Eef1akmt4-Ece2 readthrough isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC115541.2, AF489569.1, AU080537.1, AC087898.19 and AF489572.1. Transcript Variant: This variant (2, also known as 2a-1) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b, also known as 2a-1) has the same N- and C-termini but is shorter compared to isoform a. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 107522, 110599566 ##RefSeq-Attributes-END##

ATGGCCTCTC CCCGGACTCC GGTGTCCCCT CCGGAGTTAC CGGAGAAGAA CTTCCAGTAC 
CGCCAAGTCC AGTACTGGGA CCAGCGCTAC AAAGACGCGG CCGACTCTGG ACCCTACGAG 
TGGTTCGGAG ACTTCGCTTC CTTCCGTGCC CTCCTAGAGC CGGAGCTGTG TCCTGAGGAC 
CGTATCCTCG TGCTAGGTTG CGGGAACAGT GCCTTGAGCT ATGAGCTGTT CCTTGGAGGC 
TTCCCTAATG TGACCAGTGT GGATTACTCT CCAGTAGTGG TGGCAGCCAT GCAAGTTCGC 
TATGCCCATG TACCCAGTCT ACGCTGGGAG ACCATGGATG TTCGAGCACT GGACTTCCCT 
AGTGGGTCTT TTGATGTTGT CCTCGAGAAG GGTACTCTGG ATGCCATGCT GGCTGGGGAG 
CCAGATCCCT GGAATGTATC TTCTGAAGGT GTCCACACTG TGGACCAGGT GCTGAGTGAG 
GTGGGATTCC AGAAGAGGAC AAGACAACTG TTTGGTTCAC ACACACAGTT GGAGCTGGTC 
TTGGCAGGCC TCATTCTAGT GTTGGCTGCC CTTCTTTTGG GCTGCCTCGT GGCTCTGTGG 
GTCCACAGAG ACCCAGCCCA TAGCACCTGC GTCACAGAAG CCTGCATTCG AGTAGCTGGA 
AAAATCCTGG AGTCTCTAGA CCGTGGGGTG AGCCCCTGTC AGGACTTTTA CCAGTTCTCC 
TGTGGAGGCT GGATTCGAAG AAACCCTCTA CCCAATGGAC GTTCTCGCTG GAACACCTTC 
AACAGCCTCT GGGACCAGAA CCAGGCCATA CTGAAGCACC TACTTGAGAA CACCACTTTC 
AATTCCAGCA GTGAAGCTGA GAGGAAGACT CGGAGTTTCT ACCTGTCCTG CCTACAGTCG 
GAGCGCATTG AGAAGCTAGG AGCCAAGCCA CTTAGAGACC TCATTGACAA GATCGGTGGT 
TGGAACATAA CGGGGCCTTG GGACGAGGAC AGCTTCATGG ATGTGCTCAA GGCAGTCGCA 
GGGACCTACA GAGCCACCCC CTTCTTCACC GTCTACGTCA GTGCTGATTC TAAGAGTTCT 
AACAGCAATA TCATCCAGGT GGACCAGTCT GGGCTTTTTC TGCCCTCTCG AGATTACTAC 
CTAAATAGAA CTGCCAATGA GAAAGTTCTC ACTGCCTACC TGGACTACAT GGTGGAGCTG 
GGAGTGCTGC TGGGTGGACA GCCGACCTCC ACTCGGGAGC AGATGCAGCA GGTGCTGGAG 
CTGGAGATAC AGCTGGCTAA CATCACTGTG CCCCAGGACC AGCGGCGTGA TGAGGAGAAG 
ATCTATCACA AGATGAGCAT CTCAGAGCTG CAGGCTCTCG CGCCCGCCGT GGACTGGCTG 
GAGTTCCTTT CTTTCTTGTT ATCGCCACTT GAGTTGGGTG ATTCTGAGCC TGTGGTGGTG 
TATGGGACTG AGTATTTACA GCAGGTGTCG GAGCTCATCA ACCGTACTGA ACCAAGCATC 
CTGAACAATT ACCTAATTTG GAACCTGGTA CAGAAGACGA CCTCAAGCCT TGACCAGCGC 
TTTGAGACTG CACAGGAGAA ACTGCTGGAG ACCCTCTACG GTACCAAGAA GTCCTGCACT 
CCGAGGTGGC AGACCTGCAT CTCCAATACA GATGATGCCC TTGGCTTTGC TCTGGGTTCA 
CTCTTTGTGA AAGCCACATT TGACCGACAA AGCAAGGAAA TCGCCGAGGG GATGATCAAT 
GAAATCCGCT CTGCTTTTGA GGAGACCCTG GGAGACTTGG TTTGGATGGA TGAGAAGACC 
CGGCTGGCAG CCAAGGAGAA AGCAGATGCC ATCTATGATA TGATTGGTTT CCCTGATTTC 
ATCCTGGAGC CCAAAGAGCT GGATGATGTT TATGATGGGT ATGAAGTCTC TGAAGATTCT 
TTTTTCCAAA ACATGTTGAA TCTGTACAAC TTCTCAGCTA AGGTGATGGC TGACCAGCTC 
CGCAAACCTC CCAGCCGAGA CCAGTGGAGC ATGACACCTC AGACCGTGAA CGCTTACTAC 
CTTCCAACCA AGAATGAAAT CGTCTTCCCT GCTGGCATCT TGCAGGCCCC CTTCTATGCT 
CACAACCATC CAAAGGCCTT GAACTTTGGT GGCATCGGCG TAGTGATGGG CCATGAGTTG 
ACACATGCCT TTGATGACCA AGGGCGTGAG TATGACAAAG AAGGGAATCT GCGTCCTTGG 
TGGCAGAATG AGTCACTGAC GGCTTTCCAG AACCATACAG CCTGCATGGA AGAACAGTAC 
AGCCAGTACC AGGTCAATGG AGAGAGGCTC AATGGACTCC AGACCCTGGG GGAAAACATC 
GCCGATAATG GGGGCCTTAA GGCTGCTTAC AATGCTTACA AAGCATGGCT GAGAAAGCAT 
GGGGAGGAGC AGCCGCTGCC TGCTGTGGGG CTCACCAATC ACCAGCTTTT CTTCGTGGGA 
TTTGCTCAGG TGTGGTGCTC GGTCCGCACA CCAGAGAGCT CTCACGAGGG GCTGGTGACC 
GACCCCCACA GCCCTGCCCG TTTCCGAGTG CTGGGCACTC TCTCCAACTC CCGAGACTTC 
CTTCGGCACT TCGGCTGCCC TGTCGGCTCC CCCATGAACC CAGGGCAGCT ATGTGAGGTG 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808809.1
CDS	25..2670
Translation	MASPRTPVSPPELPEKNFQYRQVQYWDQRYKDAADSGPYEWFGDFASFRALLEPELCPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSPVVVAAMQVRYAHVPSLRWETMDVRALDFPSGSFDVVLEKGTLDAMLAGEPDPWNVSSEGVHTVDQVLSEVGFQKRTRQLFGSHTQLELVLAGLILVLAALLLGCLVALWVHRDPAHSTCVTEACIRVAGKILESLDRGVSPCQDFYQFSCGGWIRRNPLPNGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGWNITGPWDEDSFMDVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLETLYGTKKSCTPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVWMDEKTRLAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLTAFQNHTACMEEQYSQYQVNGERLNGLQTLGENIADNGGLKAAYNAYKAWLRKHGEEQPLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW

Target ORF information:

RefSeq Version	NM_177940.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Eef1akmt4-Ece2 readthrough (Eef1akmt4-ece2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177940.1

ORF Insert Sequence:

ATGGCCTCTC CCCGGACTCC GGTGTCCCCT CCGGAGTTAC CGGAGAAGAA CTTCCAGTAC 
CGCCAAGTCC AGTACTGGGA CCAGCGCTAC AAAGACGCGG CCGACTCTGG ACCCTACGAG 
TGGTTCGGAG ACTTCGCTTC CTTCCGTGCC CTCCTAGAGC CGGAGCTGTG TCCTGAGGAC 
CGTATCCTCG TGCTAGGTTG CGGGAACAGT GCCTTGAGCT ATGAGCTGTT CCTTGGAGGC 
TTCCCTAATG TGACCAGTGT GGATTACTCT CCAGTAGTGG TGGCAGCCAT GCAAGTTCGC 
TATGCCCATG TACCCAGTCT ACGCTGGGAG ACCATGGATG TTCGAGCACT GGACTTCCCT 
AGTGGGTCTT TTGATGTTGT CCTCGAGAAG GGTACTCTGG ATGCCATGCT GGCTGGGGAG 
CCAGATCCCT GGAATGTATC TTCTGAAGGT GTCCACACTG TGGACCAGGT GCTGAGTGAG 
GTGGGATTCC AGAAGAGGAC AAGACAACTG TTTGGTTCAC ACACACAGTT GGAGCTGGTC 
TTGGCAGGCC TCATTCTAGT GTTGGCTGCC CTTCTTTTGG GCTGCCTCGT GGCTCTGTGG 
GTCCACAGAG ACCCAGCCCA TAGCACCTGC GTCACAGAAG CCTGCATTCG AGTAGCTGGA 
AAAATCCTGG AGTCTCTAGA CCGTGGGGTG AGCCCCTGTC AGGACTTTTA CCAGTTCTCC 
TGTGGAGGCT GGATTCGAAG AAACCCTCTA CCCAATGGAC GTTCTCGCTG GAACACCTTC 
AACAGCCTCT GGGACCAGAA CCAGGCCATA CTGAAGCACC TACTTGAGAA CACCACTTTC 
AATTCCAGCA GTGAAGCTGA GAGGAAGACT CGGAGTTTCT ACCTGTCCTG CCTACAGTCG 
GAGCGCATTG AGAAGCTAGG AGCCAAGCCA CTTAGAGACC TCATTGACAA GATCGGTGGT 
TGGAACATAA CGGGGCCTTG GGACGAGGAC AGCTTCATGG ATGTGCTCAA GGCAGTCGCA 
GGGACCTACA GAGCCACCCC CTTCTTCACC GTCTACGTCA GTGCTGATTC TAAGAGTTCT 
AACAGCAATA TCATCCAGGT GGACCAGTCT GGGCTTTTTC TGCCCTCTCG AGATTACTAC 
CTAAATAGAA CTGCCAATGA GAAAGTTCTC ACTGCCTACC TGGACTACAT GGTGGAGCTG 
GGAGTGCTGC TGGGTGGACA GCCGACCTCC ACTCGGGAGC AGATGCAGCA GGTGCTGGAG 
CTGGAGATAC AGCTGGCTAA CATCACTGTG CCCCAGGACC AGCGGCGTGA TGAGGAGAAG 
ATCTATCACA AGATGAGCAT CTCAGAGCTG CAGGCTCTCG CGCCCGCCGT GGACTGGCTG 
GAGTTCCTTT CTTTCTTGTT ATCGCCACTT GAGTTGGGTG ATTCTGAGCC TGTGGTGGTG 
TATGGGACTG AGTATTTACA GCAGGTGTCG GAGCTCATCA ACCGTACTGA ACCAAGCATC 
CTGAACAATT ACCTAATTTG GAACCTGGTA CAGAAGACGA CCTCAAGCCT TGACCAGCGC 
TTTGAGACTG CACAGGAGAA ACTGCTGGAG ACCCTCTACG GTACCAAGAA GTCCTGCACT 
CCGAGGTGGC AGACCTGCAT CTCCAATACA GATGATGCCC TTGGCTTTGC TCTGGGTTCA 
CTCTTTGTGA AAGCCACATT TGACCGACAA AGCAAGGAAA TCGCCGAGGG GATGATCAAT 
GAAATCCGCT CTGCTTTTGA GGAGACCCTG GGAGACTTGG TTTGGATGGA TGAGAAGACC 
CGGCTGGCAG CCAAGGAGAA AGCAGATGCC ATCTATGATA TGATTGGTTT CCCTGATTTC 
ATCCTGGAGC CCAAAGAGCT GGATGATGTT TATGATGGGT ATGAAGTCTC TGAAGATTCT 
TTTTTCCAAA ACATGTTGAA TCTGTACAAC TTCTCAGCTA AGGTGATGGC TGACCAGCTC 
CGCAAACCTC CCAGCCGAGA CCAGTGGAGC ATGACACCTC AGACCGTGAA CGCTTACTAC 
CTTCCAACCA AGAATGAAAT CGTCTTCCCT GCTGGCATCT TGCAGGCCCC CTTCTATGCT 
CACAACCATC CAAAGGCCTT GAACTTTGGT GGCATCGGCG TAGTGATGGG CCATGAGTTG 
ACACATGCCT TTGATGACCA AGGGCGTGAG TATGACAAAG AAGGGAATCT GCGTCCTTGG 
TGGCAGAATG AGTCACTGAC GGCTTTCCAG AACCATACAG CCTGCATGGA AGAACAGTAC 
AGCCAGTACC AGGTCAATGG AGAGAGGCTC AATGGACTCC AGACCCTGGG GGAAAACATC 
GCCGATAATG GGGGCCTTAA GGCTGCTTAC AATGCTTACA AAGCATGGCT GAGAAAGCAT 
GGGGAGGAGC AGCCGCTGCC TGCTGTGGGG CTCACCAATC ACCAGCTTTT CTTCGTGGGA 
TTTGCTCAGG TGTGGTGCTC GGTCCGCACA CCAGAGAGCT CTCACGAGGG GCTGGTGACC 
GACCCCCACA GCCCTGCCCG TTTCCGAGTG CTGGGCACTC TCTCCAACTC CCGAGACTTC 
CTTCGGCACT TCGGCTGCCC TGTCGGCTCC CCCATGAACC CAGGGCAGCT ATGTGAGGTG 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eef1akmt4-ece2 ( NM_177941.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808810.1 Mus musculus Eef1akmt4-Ece2 readthrough (Eef1akmt4-ece2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07050
Clone ID Related Accession (Same CDS sequence)	NM_177941.1
Accession Version	NM_177941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	Eef1akmt4-Ece2 readthrough isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC115541.2, AF489570.1, AU080537.1, AC087898.19 and AF489572.1. Transcript Variant: This variant (1, also known as 2a-2) represents the longer transcript and encodes the longer isoform (a, also known as 2a-2). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 107522, 110599566 ##RefSeq-Attributes-END##

ATGGCCTCTC CCCGGACTCC GGTGTCCCCT CCGGAGTTAC CGGAGAAGAA CTTCCAGTAC 
CGCCAAGTCC AGTACTGGGA CCAGCGCTAC AAAGACGCGG CCGACTCTGG ACCCTACGAG 
TGGTTCGGAG ACTTCGCTTC CTTCCGTGCC CTCCTAGAGC CGGAGCTGTG TCCTGAGGAC 
CGTATCCTCG TGCTAGGTTG CGGGAACAGT GCCTTGAGCT ATGAGCTGTT CCTTGGAGGC 
TTCCCTAATG TGACCAGTGT GGATTACTCT CCAGTAGTGG TGGCAGCCAT GCAAGTTCGC 
TATGCCCATG TACCCAGTCT ACGCTGGGAG ACCATGGATG TTCGAGCACT GGACTTCCCT 
AGTGGGTCTT TTGATGTTGT CCTCGAGAAG GGTACTCTGG ATGCCATGCT GGCTGGGGAG 
CCAGATCCCT GGAATGTATC TTCTGAAGGT GTCCACACTG TGGACCAGGT GCTGAGTGAG 
ATGGTGGAGT ACAAACGTGC TAAGCTTCGG GATGAAGAAT CACCAGAGAT TACAGTTGAA 
GGCAGGGCCA CCCGGGACTC ACTGGAGGTG GGATTCCAGA AGAGGACAAG ACAACTGTTT 
GGTTCACACA CACAGTTGGA GCTGGTCTTG GCAGGCCTCA TTCTAGTGTT GGCTGCCCTT 
CTTTTGGGCT GCCTCGTGGC TCTGTGGGTC CACAGAGACC CAGCCCATAG CACCTGCGTC 
ACAGAAGCCT GCATTCGAGT AGCTGGAAAA ATCCTGGAGT CTCTAGACCG TGGGGTGAGC 
CCCTGTCAGG ACTTTTACCA GTTCTCCTGT GGAGGCTGGA TTCGAAGAAA CCCTCTACCC 
AATGGACGTT CTCGCTGGAA CACCTTCAAC AGCCTCTGGG ACCAGAACCA GGCCATACTG 
AAGCACCTAC TTGAGAACAC CACTTTCAAT TCCAGCAGTG AAGCTGAGAG GAAGACTCGG 
AGTTTCTACC TGTCCTGCCT ACAGTCGGAG CGCATTGAGA AGCTAGGAGC CAAGCCACTT 
AGAGACCTCA TTGACAAGAT CGGTGGTTGG AACATAACGG GGCCTTGGGA CGAGGACAGC 
TTCATGGATG TGCTCAAGGC AGTCGCAGGG ACCTACAGAG CCACCCCCTT CTTCACCGTC 
TACGTCAGTG CTGATTCTAA GAGTTCTAAC AGCAATATCA TCCAGGTGGA CCAGTCTGGG 
CTTTTTCTGC CCTCTCGAGA TTACTACCTA AATAGAACTG CCAATGAGAA AGTTCTCACT 
GCCTACCTGG ACTACATGGT GGAGCTGGGA GTGCTGCTGG GTGGACAGCC GACCTCCACT 
CGGGAGCAGA TGCAGCAGGT GCTGGAGCTG GAGATACAGC TGGCTAACAT CACTGTGCCC 
CAGGACCAGC GGCGTGATGA GGAGAAGATC TATCACAAGA TGAGCATCTC AGAGCTGCAG 
GCTCTCGCGC CCGCCGTGGA CTGGCTGGAG TTCCTTTCTT TCTTGTTATC GCCACTTGAG 
TTGGGTGATT CTGAGCCTGT GGTGGTGTAT GGGACTGAGT ATTTACAGCA GGTGTCGGAG 
CTCATCAACC GTACTGAACC AAGCATCCTG AACAATTACC TAATTTGGAA CCTGGTACAG 
AAGACGACCT CAAGCCTTGA CCAGCGCTTT GAGACTGCAC AGGAGAAACT GCTGGAGACC 
CTCTACGGTA CCAAGAAGTC CTGCACTCCG AGGTGGCAGA CCTGCATCTC CAATACAGAT 
GATGCCCTTG GCTTTGCTCT GGGTTCACTC TTTGTGAAAG CCACATTTGA CCGACAAAGC 
AAGGAAATCG CCGAGGGGAT GATCAATGAA ATCCGCTCTG CTTTTGAGGA GACCCTGGGA 
GACTTGGTTT GGATGGATGA GAAGACCCGG CTGGCAGCCA AGGAGAAAGC AGATGCCATC 
TATGATATGA TTGGTTTCCC TGATTTCATC CTGGAGCCCA AAGAGCTGGA TGATGTTTAT 
GATGGGTATG AAGTCTCTGA AGATTCTTTT TTCCAAAACA TGTTGAATCT GTACAACTTC 
TCAGCTAAGG TGATGGCTGA CCAGCTCCGC AAACCTCCCA GCCGAGACCA GTGGAGCATG 
ACACCTCAGA CCGTGAACGC TTACTACCTT CCAACCAAGA ATGAAATCGT CTTCCCTGCT 
GGCATCTTGC AGGCCCCCTT CTATGCTCAC AACCATCCAA AGGCCTTGAA CTTTGGTGGC 
ATCGGCGTAG TGATGGGCCA TGAGTTGACA CATGCCTTTG ATGACCAAGG GCGTGAGTAT 
GACAAAGAAG GGAATCTGCG TCCTTGGTGG CAGAATGAGT CACTGACGGC TTTCCAGAAC 
CATACAGCCT GCATGGAAGA ACAGTACAGC CAGTACCAGG TCAATGGAGA GAGGCTCAAT 
GGACTCCAGA CCCTGGGGGA AAACATCGCC GATAATGGGG GCCTTAAGGC TGCTTACAAT 
GCTTACAAAG CATGGCTGAG AAAGCATGGG GAGGAGCAGC CGCTGCCTGC TGTGGGGCTC 
ACCAATCACC AGCTTTTCTT CGTGGGATTT GCTCAGGTGT GGTGCTCGGT CCGCACACCA 
GAGAGCTCTC ACGAGGGGCT GGTGACCGAC CCCCACAGCC CTGCCCGTTT CCGAGTGCTG 
GGCACTCTCT CCAACTCCCG AGACTTCCTT CGGCACTTCG GCTGCCCTGT CGGCTCCCCC 
ATGAACCCAG GGCAGCTATG TGAGGTGTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808810.1
CDS	25..2757
Translation	MASPRTPVSPPELPEKNFQYRQVQYWDQRYKDAADSGPYEWFGDFASFRALLEPELCPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSPVVVAAMQVRYAHVPSLRWETMDVRALDFPSGSFDVVLEKGTLDAMLAGEPDPWNVSSEGVHTVDQVLSEMVEYKRAKLRDEESPEITVEGRATRDSLEVGFQKRTRQLFGSHTQLELVLAGLILVLAALLLGCLVALWVHRDPAHSTCVTEACIRVAGKILESLDRGVSPCQDFYQFSCGGWIRRNPLPNGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGWNITGPWDEDSFMDVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLETLYGTKKSCTPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVWMDEKTRLAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLTAFQNHTACMEEQYSQYQVNGERLNGLQTLGENIADNGGLKAAYNAYKAWLRKHGEEQPLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW

Target ORF information:

RefSeq Version	NM_177941.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Eef1akmt4-Ece2 readthrough (Eef1akmt4-ece2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177941.1

ORF Insert Sequence:

ATGGCCTCTC CCCGGACTCC GGTGTCCCCT CCGGAGTTAC CGGAGAAGAA CTTCCAGTAC 
CGCCAAGTCC AGTACTGGGA CCAGCGCTAC AAAGACGCGG CCGACTCTGG ACCCTACGAG 
TGGTTCGGAG ACTTCGCTTC CTTCCGTGCC CTCCTAGAGC CGGAGCTGTG TCCTGAGGAC 
CGTATCCTCG TGCTAGGTTG CGGGAACAGT GCCTTGAGCT ATGAGCTGTT CCTTGGAGGC 
TTCCCTAATG TGACCAGTGT GGATTACTCT CCAGTAGTGG TGGCAGCCAT GCAAGTTCGC 
TATGCCCATG TACCCAGTCT ACGCTGGGAG ACCATGGATG TTCGAGCACT GGACTTCCCT 
AGTGGGTCTT TTGATGTTGT CCTCGAGAAG GGTACTCTGG ATGCCATGCT GGCTGGGGAG 
CCAGATCCCT GGAATGTATC TTCTGAAGGT GTCCACACTG TGGACCAGGT GCTGAGTGAG 
ATGGTGGAGT ACAAACGTGC TAAGCTTCGG GATGAAGAAT CACCAGAGAT TACAGTTGAA 
GGCAGGGCCA CCCGGGACTC ACTGGAGGTG GGATTCCAGA AGAGGACAAG ACAACTGTTT 
GGTTCACACA CACAGTTGGA GCTGGTCTTG GCAGGCCTCA TTCTAGTGTT GGCTGCCCTT 
CTTTTGGGCT GCCTCGTGGC TCTGTGGGTC CACAGAGACC CAGCCCATAG CACCTGCGTC 
ACAGAAGCCT GCATTCGAGT AGCTGGAAAA ATCCTGGAGT CTCTAGACCG TGGGGTGAGC 
CCCTGTCAGG ACTTTTACCA GTTCTCCTGT GGAGGCTGGA TTCGAAGAAA CCCTCTACCC 
AATGGACGTT CTCGCTGGAA CACCTTCAAC AGCCTCTGGG ACCAGAACCA GGCCATACTG 
AAGCACCTAC TTGAGAACAC CACTTTCAAT TCCAGCAGTG AAGCTGAGAG GAAGACTCGG 
AGTTTCTACC TGTCCTGCCT ACAGTCGGAG CGCATTGAGA AGCTAGGAGC CAAGCCACTT 
AGAGACCTCA TTGACAAGAT CGGTGGTTGG AACATAACGG GGCCTTGGGA CGAGGACAGC 
TTCATGGATG TGCTCAAGGC AGTCGCAGGG ACCTACAGAG CCACCCCCTT CTTCACCGTC 
TACGTCAGTG CTGATTCTAA GAGTTCTAAC AGCAATATCA TCCAGGTGGA CCAGTCTGGG 
CTTTTTCTGC CCTCTCGAGA TTACTACCTA AATAGAACTG CCAATGAGAA AGTTCTCACT 
GCCTACCTGG ACTACATGGT GGAGCTGGGA GTGCTGCTGG GTGGACAGCC GACCTCCACT 
CGGGAGCAGA TGCAGCAGGT GCTGGAGCTG GAGATACAGC TGGCTAACAT CACTGTGCCC 
CAGGACCAGC GGCGTGATGA GGAGAAGATC TATCACAAGA TGAGCATCTC AGAGCTGCAG 
GCTCTCGCGC CCGCCGTGGA CTGGCTGGAG TTCCTTTCTT TCTTGTTATC GCCACTTGAG 
TTGGGTGATT CTGAGCCTGT GGTGGTGTAT GGGACTGAGT ATTTACAGCA GGTGTCGGAG 
CTCATCAACC GTACTGAACC AAGCATCCTG AACAATTACC TAATTTGGAA CCTGGTACAG 
AAGACGACCT CAAGCCTTGA CCAGCGCTTT GAGACTGCAC AGGAGAAACT GCTGGAGACC 
CTCTACGGTA CCAAGAAGTC CTGCACTCCG AGGTGGCAGA CCTGCATCTC CAATACAGAT 
GATGCCCTTG GCTTTGCTCT GGGTTCACTC TTTGTGAAAG CCACATTTGA CCGACAAAGC 
AAGGAAATCG CCGAGGGGAT GATCAATGAA ATCCGCTCTG CTTTTGAGGA GACCCTGGGA 
GACTTGGTTT GGATGGATGA GAAGACCCGG CTGGCAGCCA AGGAGAAAGC AGATGCCATC 
TATGATATGA TTGGTTTCCC TGATTTCATC CTGGAGCCCA AAGAGCTGGA TGATGTTTAT 
GATGGGTATG AAGTCTCTGA AGATTCTTTT TTCCAAAACA TGTTGAATCT GTACAACTTC 
TCAGCTAAGG TGATGGCTGA CCAGCTCCGC AAACCTCCCA GCCGAGACCA GTGGAGCATG 
ACACCTCAGA CCGTGAACGC TTACTACCTT CCAACCAAGA ATGAAATCGT CTTCCCTGCT 
GGCATCTTGC AGGCCCCCTT CTATGCTCAC AACCATCCAA AGGCCTTGAA CTTTGGTGGC 
ATCGGCGTAG TGATGGGCCA TGAGTTGACA CATGCCTTTG ATGACCAAGG GCGTGAGTAT 
GACAAAGAAG GGAATCTGCG TCCTTGGTGG CAGAATGAGT CACTGACGGC TTTCCAGAAC 
CATACAGCCT GCATGGAAGA ACAGTACAGC CAGTACCAGG TCAATGGAGA GAGGCTCAAT 
GGACTCCAGA CCCTGGGGGA AAACATCGCC GATAATGGGG GCCTTAAGGC TGCTTACAAT 
GCTTACAAAG CATGGCTGAG AAAGCATGGG GAGGAGCAGC CGCTGCCTGC TGTGGGGCTC 
ACCAATCACC AGCTTTTCTT CGTGGGATTT GCTCAGGTGT GGTGCTCGGT CCGCACACCA 
GAGAGCTCTC ACGAGGGGCT GGTGACCGAC CCCCACAGCC CTGCCCGTTT CCGAGTGCTG 
GGCACTCTCT CCAACTCCCG AGACTTCCTT CGGCACTTCG GCTGCCCTGT CGGCTCCCCC 
ATGAACCCAG GGCAGCTATG TGAGGTGTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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Eef1akmt4-ece2 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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