gRNAs
The following Manba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Manba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu31561 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006500908.2 , XM_006500908.3 | |
| Accession Version | XM_006500908.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | beta-mannosidase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGACAATTC TCTTTGCTCT GGATGGAGGC TTAAAAATTG AGAAAGCAGC CAAGGTTTAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006500971.1 |
| CDS | 1077..2810 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_006500908.2 |
1 | ATGACAATTC TCTTTGCTCT GGATGGAGGC TTAAAAATTG AGAAAGCAGC CAAGGTTTAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu03984 | |
| Clone ID Related Accession (Same CDS sequence) | NM_027288.3 | |
| Accession Version | NM_027288.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-25 | |
| Organism | Mus musculus(house mouse) | |
| Product | beta-mannosidase precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF306557.1, BB782198.1 and BU757579.1. On Oct 23, 2007 this sequence version replaced NM_027288.2. ##Evidence-Data-START## Transcript exon combination :: BC031409.1, AK166022.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCATCTTC ACCTGCTCTT GATATTGGCG CTATTTCGGG CCGGCTGCGT GGTGGCAGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_081564.3 |
| CDS | 71..2710 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_027288.3 |
1 | ATGCATCTTC ACCTGCTCTT GATATTGGCG CTATTTCGGG CCGGCTGCGT GGTGGCAGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu31561 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006500908.2 , XM_006500908.3 | |
| Accession Version | XM_006500908.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | beta-mannosidase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGACAATTC TCTTTGCTCT GGATGGAGGC TTAAAAATTG AGAAAGCAGC CAAGGTTTAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006500971.1 |
| CDS | 1077..2810 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006500908.3 |
1 | ATGACAATTC TCTTTGCTCT GGATGGAGGC TTAAAAATTG AGAAAGCAGC CAAGGTTTAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Deterministic and stochastic allele specific gene expression in single mouse blastomeres. |
Mice lacking mannose 6-phosphate uncovering enzyme activity have a milder phenotype than mice deficient for N-acetylglucosamine-1-phosphotransferase activity. |
Beta-mannosidosis mice: a model for the human lysosomal storage disease. |
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. |
