Nop2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nop2
Entrez Gene ID	110109
Full Name	NOP2 nucleolar protein
Synonyms	120kDa,A530002O17,Nol1
General protein information	Preferred Names NOP2 nucleolar protein Names probable 28S rRNA (cytosine-C(5))-methyltransferase NOP2 nucleolar protein homolog nucleolar protein 1, 120 kDa nucleolar protein 2 homolog proliferating-cell nucleolar antigen p120 proliferation-associated nucleolar protein p120 putative ribosomal RNA methyltransferase NOP2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 F2\|6 59.32 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017321322.1	XP_017176811.1	probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X1
XM_030255097.1	XP_030110957.1	probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X1
NM_138747.2	NP_620086.2	probable 28S rRNA (cytosine-C(5))-methyltransferase
XM_017321323.1	XP_017176812.1	probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NOP2	XP_005637289.1
Rattus norvegicus (Norway rat)	Nop2	NP_001178714.1
Pan troglodytes (chimpanzee)	NOP2	XP_003952139.1
Mus musculus (house mouse)	Nop2	NP_620086.2
Gallus gallus (chicken)	NOP2	XP_416502.3
Homo sapiens (human)	NOP2	NP_001245237.1
Danio rerio (zebrafish)	nop2	XP_005157997.1
	NOP2	XP_001106035.1
Bos taurus (cattle)	NOP2	NP_001095727.1

Gene Ontology
Bibliography

Related articles in PubMed

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Cui W, Dai X, Marcho C, Han Z, Zhang K, Tremblay KD, Mager J
Scientific reports637396(2016 Nov)

Nop2 is required for mammalian preimplantation development.
Cui W, Pizzollo J, Han Z, Marcho C, Zhang K, Mager J
Molecular reproduction and development83(2)124-31(2016 Feb)

Nop2 is expressed during proliferation of neural stem cells and in adult mouse and human brain.
Kosi N, Ali? I, Kola?evi? M, Vrsaljko N, Jovanov Milo?evi? N, Sobol M, Philimonenko A, Hoz?k P, Gajovi? S, Pochet R, Mitre?i? D
Brain research159765-76(2015 Feb)

The AERO system: a 3D-like approach for recording gene expression patterns in the whole mouse embryo.
Shimizu H, Kubo A, Uchibe K, Hashimoto M, Yokoyama S, Takada S, Mitsuoka K, Asahara H
PloS one8(10)e75754(2013)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate that Nop2 is an essential gene for blastocyst formation, and is required for RNA processing and/or stability in vivo during preimplantation embryo development in the mouse.
Title: Nop2 is required for mammalian preimplantation development.

Findings suggest a newly discovered role of Nop2 in both mature neurons and in cells possibly involved in the regeneration of nervous tissue
Title: Nop2 is expressed during proliferation of neural stem cells and in adult mouse and human brain.

Inappropriate expression of the p120 gene can result in aberrant neutrophil maturation.
Title: p120 nucleolar-proliferating antigen is a direct target of G-CSF signaling during myeloid differentiation.

The following Nop2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nop2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21100	NM_138747.2 Latest version!	Mus musculus NOP2 nucleolar protein (Nop2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu21100	XM_030255097.1 Latest version!	Mus musculus NOP2 nucleolar protein (Nop2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu81613	XM_017321323.1 Latest version!	Mus musculus NOP2 nucleolar protein (Nop2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu81613	XM_017321322.1 Latest version!	Mus musculus NOP2 nucleolar protein (Nop2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nop2 ( NM_138747.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_620086.2 Mus musculus NOP2 nucleolar protein (Nop2), mRNA.

Price & Availability↑

CloneID	OMu21100
Clone ID Related Accession (Same CDS sequence)	XM_030255097.1 , NM_138747.2
Accession Version	NM_138747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	probable 28S rRNA (cytosine-C(5))-methyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY244994.1, AK079914.1 and AI325393.1. On Nov 1, 2007 this sequence version replaced NM_138747.1. ##Evidence-Data-START## Transcript exon combination :: BC007151.1, AK079914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCGCA AGTTGGATCC TACGAAGAAG GAGAAGCGTG GACCCGGCCG AAAGGCCCGA 
AAACAGAAGG GTGCGGAGAC CGAATTGGTC AGATTTTTGC CTGCAGCTGG CGATGAGAAT 
TCCAAGAGGC TGTCGAGTCG TGCCCGAAAG CGGGCAGCCA AGAGGAGGGC AGGGTCTGTT 
GACGTCCCTA AGCCAAATAA GTCTCCTGGG ATCAAAACAT TGCCTGGAGA GTTATCCAAA 
GGAGCAGTCC AGGCTCGAGG TAAGAAGCGC CCAGCACCAA TTCAAAACAG TGATGGGGAC 
GAGGAGGAAG ACTCGGGGGA AGACGATGTG GTGACCCAGG GGGACCTTTG GGGCTCTGAG 
GACAGTGATG AAGATATGGT GGATGACTAT GGAGCTGCCT CTAACTCGGA GGATGAGGAG 
GAAAAGCTGT TGCCTATTGA AAGAGCTGCC CTGAAGCAGA AAGCCCAGGA TGCCACAGCT 
GGGGTCCTGT GGAATGAAGA GGACACTGAT GAAGATGAAG ATGATGATGG TGTGTCCCCT 
GAGTCCCACC CCAGAAAGGA TGACAAAGCA GAGGGGGATT TGCAGATTAA TGTGGAAGAT 
GAGGAAGCCT TTGTGCTGCC CCCTGCTGGG GAGACGGACC AGGATGGCCA GGCTCCAGAC 
CTGCAGCGAG TTCACAAGCG GATCCAGGAC ATCGTGGGAG TGCTGCGGGA TTTCGGAGCT 
CAGCGAGAAG AGGGCCGGTC TCGAGCTGAG TATCTAAGTC GGCTTCAGAA GGACCTGGCC 
ACTTACTATT CTTATGGAGA CTTCCTGCTG AGCAAGCTCA TGGAGCTGTT CCCTCTGTCT 
GAGTTGATCG AGTTCTTAGA AGCTAATGAG GTGCCCCGGC CAATCACCCT TCGGACCAAC 
ACCTTGAAAA CCCGTCGCCG AGACCTTGCT CAGGCTCTGA TCAATCGTGG GGTTAATCTG 
GATCCACTGG GGAAGTGGTC AAAGTCTGGA CTTGTGGTAT ATGATTCTTC AGTGCCTATT 
GGCGCTACCC CTGAGTACCT CGCTGGACAC TATATGCTGC AGGGAGCTTC CAGTATGTTG 
CCCGTCATGG CCCTGGCACC TCAGGAGCAT GAGCGGATCT TAGACATGTG CTGTGCTCCT 
GGGGGGAAGA CCAGCTACAT AGCTCAGCTG ATGAAGAACA CAGGAGTGAT CCTTGCCAAC 
GATGCCAATG CAGACCGGCT CAAGAGCGTG GTGGGCAACC TGCACCGCTT GGGAGTCACC 
AACACCATCA TCAGCCACTA TGATGGGCGC CAGTTCCCCA AGGTGGTAGG GGGCTTTGAT 
CGAGTGCTAC TGGATGCTCC TTGTAGTGGC ACAGGAGTCA TCTCCAAAGA CCCTGCTGTG 
AAGACGAACA AGGATGAGAA AGACATCCAG CGCTGTGCCC ACCTTCAGAA GGAACTGCTG 
CTCAGTGCTA TCGACTCAGT GAACGCTGCC TCCAAGACCG GAGGCTACCT GGTCTACTGC 
ACCTGTTCCA TTACGGTGGA AGAGAACGAG TGGGTGGTAG ACTATGCCTT GAAGAAGAGG 
AATGTGCGGC TGGTGCCCAC CGGCCTGGAC TTCGGCCAGG AAGGTTTTAC CCGCTTCCAA 
GCAAGGCGCT TTCACCCCAC TCTGCGTTCC ACCCGGCGCT TCTACCCTCA CACTCACAAT 
ATGGACGGTT TCTTTATCGC CAAGTTCAAG AAATTTTCCA ATTCTATTCC CCAGCCCCAT 
GCAGGAAACT CGGCAGCAGC TACCCCTACA GAGCCTGACC TGAAGGATCA GGTCACCCCA 
AAGTCTGAGA ACGGTAGTCA GCCCACCAAG AAAGCCCGTG GGGCTGTGAA GGCAAAGCAG 
CAGTTGCTGA GACAGCCACA TTCCAAGAAG CCCTTCCAGA AGTTGAACGG CATCGCCAAA 
GGGCCAGGCT TGTCCACTGA ACCTTCTGTC CCAGATGCCC AAGTGTCTAC CAGGCCCAGT 
CAGTCTGCTG GGAATGCTGA CGTGAACAGC AAACGAAAGA GGTCTGAGAA GCTGAAGCAG 
CGGGGACCTA AGTGGAAGCC CTCTAAGGAG GCTGCTGTCC CAAAGCCGAG CGCCCCTTCC 
AGGGTTGAGG ACTCGAGAAC ACCTCCTGTG CCGACTCCCT CTGAAATCCG TGCTGCCCCA 
AGGCCTAAGG ACTGTGCTCC ATCCCTTGGG GAAGCCAAAA AGAAACAGAA AGGAAAGCAG 
CAGTTAGCAC AGCAGCCTGC TAATGGAGCT GCTCCCCTGA AAGAAGATGC TGTTTCCAAG 
GGACCCTCAG CCCCCTTTGT GTCTCCCCAC AGTTCCACCA GGCCCCCACC AGCAAAAAGG 
AGGAAATCAA TGACAAAGGG CAACAGCCAG CCTCTGTTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620086.2
CDS	127..2511
Translation	MGRKLDPTKKEKRGPGRKARKQKGAETELVRFLPAAGDENSKRLSSRARKRAAKRRAGSVDVPKPNKSPGIKTLPGELSKGAVQARGKKRPAPIQNSDGDEEEDSGEDDVVTQGDLWGSEDSDEDMVDDYGAASNSEDEEEKLLPIERAALKQKAQDATAGVLWNEEDTDEDEDDDGVSPESHPRKDDKAEGDLQINVEDEEAFVLPPAGETDQDGQAPDLQRVHKRIQDIVGVLRDFGAQREEGRSRAEYLSRLQKDLATYYSYGDFLLSKLMELFPLSELIEFLEANEVPRPITLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKSGLVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYIAQLMKNTGVILANDANADRLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDIQRCAHLQKELLLSAIDSVNAASKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFQARRFHPTLRSTRRFYPHTHNMDGFFIAKFKKFSNSIPQPHAGNSAAATPTEPDLKDQVTPKSENGSQPTKKARGAVKAKQQLLRQPHSKKPFQKLNGIAKGPGLSTEPSVPDAQVSTRPSQSAGNADVNSKRKRSEKLKQRGPKWKPSKEAAVPKPSAPSRVEDSRTPPVPTPSEIRAAPRPKDCAPSLGEAKKKQKGKQQLAQQPANGAAPLKEDAVSKGPSAPFVSPHSSTRPPPAKRRKSMTKGNSQPLLS

Target ORF information:

RefSeq Version	NM_138747.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NOP2 nucleolar protein (Nop2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138747.2

ORF Insert Sequence:

ATGGGGCGCA AGTTGGATCC TACGAAGAAG GAGAAGCGTG GACCCGGCCG AAAGGCCCGA 
AAACAGAAGG GTGCGGAGAC CGAATTGGTC AGATTTTTGC CTGCAGCTGG CGATGAGAAT 
TCCAAGAGGC TGTCGAGTCG TGCCCGAAAG CGGGCAGCCA AGAGGAGGGC AGGGTCTGTT 
GACGTCCCTA AGCCAAATAA GTCTCCTGGG ATCAAAACAT TGCCTGGAGA GTTATCCAAA 
GGAGCAGTCC AGGCTCGAGG TAAGAAGCGC CCAGCACCAA TTCAAAACAG TGATGGGGAC 
GAGGAGGAAG ACTCGGGGGA AGACGATGTG GTGACCCAGG GGGACCTTTG GGGCTCTGAG 
GACAGTGATG AAGATATGGT GGATGACTAT GGAGCTGCCT CTAACTCGGA GGATGAGGAG 
GAAAAGCTGT TGCCTATTGA AAGAGCTGCC CTGAAGCAGA AAGCCCAGGA TGCCACAGCT 
GGGGTCCTGT GGAATGAAGA GGACACTGAT GAAGATGAAG ATGATGATGG TGTGTCCCCT 
GAGTCCCACC CCAGAAAGGA TGACAAAGCA GAGGGGGATT TGCAGATTAA TGTGGAAGAT 
GAGGAAGCCT TTGTGCTGCC CCCTGCTGGG GAGACGGACC AGGATGGCCA GGCTCCAGAC 
CTGCAGCGAG TTCACAAGCG GATCCAGGAC ATCGTGGGAG TGCTGCGGGA TTTCGGAGCT 
CAGCGAGAAG AGGGCCGGTC TCGAGCTGAG TATCTAAGTC GGCTTCAGAA GGACCTGGCC 
ACTTACTATT CTTATGGAGA CTTCCTGCTG AGCAAGCTCA TGGAGCTGTT CCCTCTGTCT 
GAGTTGATCG AGTTCTTAGA AGCTAATGAG GTGCCCCGGC CAATCACCCT TCGGACCAAC 
ACCTTGAAAA CCCGTCGCCG AGACCTTGCT CAGGCTCTGA TCAATCGTGG GGTTAATCTG 
GATCCACTGG GGAAGTGGTC AAAGTCTGGA CTTGTGGTAT ATGATTCTTC AGTGCCTATT 
GGCGCTACCC CTGAGTACCT CGCTGGACAC TATATGCTGC AGGGAGCTTC CAGTATGTTG 
CCCGTCATGG CCCTGGCACC TCAGGAGCAT GAGCGGATCT TAGACATGTG CTGTGCTCCT 
GGGGGGAAGA CCAGCTACAT AGCTCAGCTG ATGAAGAACA CAGGAGTGAT CCTTGCCAAC 
GATGCCAATG CAGACCGGCT CAAGAGCGTG GTGGGCAACC TGCACCGCTT GGGAGTCACC 
AACACCATCA TCAGCCACTA TGATGGGCGC CAGTTCCCCA AGGTGGTAGG GGGCTTTGAT 
CGAGTGCTAC TGGATGCTCC TTGTAGTGGC ACAGGAGTCA TCTCCAAAGA CCCTGCTGTG 
AAGACGAACA AGGATGAGAA AGACATCCAG CGCTGTGCCC ACCTTCAGAA GGAACTGCTG 
CTCAGTGCTA TCGACTCAGT GAACGCTGCC TCCAAGACCG GAGGCTACCT GGTCTACTGC 
ACCTGTTCCA TTACGGTGGA AGAGAACGAG TGGGTGGTAG ACTATGCCTT GAAGAAGAGG 
AATGTGCGGC TGGTGCCCAC CGGCCTGGAC TTCGGCCAGG AAGGTTTTAC CCGCTTCCAA 
GCAAGGCGCT TTCACCCCAC TCTGCGTTCC ACCCGGCGCT TCTACCCTCA CACTCACAAT 
ATGGACGGTT TCTTTATCGC CAAGTTCAAG AAATTTTCCA ATTCTATTCC CCAGCCCCAT 
GCAGGAAACT CGGCAGCAGC TACCCCTACA GAGCCTGACC TGAAGGATCA GGTCACCCCA 
AAGTCTGAGA ACGGTAGTCA GCCCACCAAG AAAGCCCGTG GGGCTGTGAA GGCAAAGCAG 
CAGTTGCTGA GACAGCCACA TTCCAAGAAG CCCTTCCAGA AGTTGAACGG CATCGCCAAA 
GGGCCAGGCT TGTCCACTGA ACCTTCTGTC CCAGATGCCC AAGTGTCTAC CAGGCCCAGT 
CAGTCTGCTG GGAATGCTGA CGTGAACAGC AAACGAAAGA GGTCTGAGAA GCTGAAGCAG 
CGGGGACCTA AGTGGAAGCC CTCTAAGGAG GCTGCTGTCC CAAAGCCGAG CGCCCCTTCC 
AGGGTTGAGG ACTCGAGAAC ACCTCCTGTG CCGACTCCCT CTGAAATCCG TGCTGCCCCA 
AGGCCTAAGG ACTGTGCTCC ATCCCTTGGG GAAGCCAAAA AGAAACAGAA AGGAAAGCAG 
CAGTTAGCAC AGCAGCCTGC TAATGGAGCT GCTCCCCTGA AAGAAGATGC TGTTTCCAAG 
GGACCCTCAG CCCCCTTTGT GTCTCCCCAC AGTTCCACCA GGCCCCCACC AGCAAAAAGG 
AGGAAATCAA TGACAAAGGG CAACAGCCAG CCTCTGTTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nop2 ( XM_030255097.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110957.1 Mus musculus NOP2 nucleolar protein (Nop2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21100
Clone ID Related Accession (Same CDS sequence)	XM_030255097.1 , NM_138747.2
Accession Version	XM_030255097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGCA AGTTGGATCC TACGAAGAAG GAGAAGCGTG GACCCGGCCG AAAGGCCCGA 
AAACAGAAGG GTGCGGAGAC CGAATTGGTC AGATTTTTGC CTGCAGCTGG CGATGAGAAT 
TCCAAGAGGC TGTCGAGTCG TGCCCGAAAG CGGGCAGCCA AGAGGAGGGC AGGGTCTGTT 
GACGTCCCTA AGCCAAATAA GTCTCCTGGG ATCAAAACAT TGCCTGGAGA GTTATCCAAA 
GGAGCAGTCC AGGCTCGAGG TAAGAAGCGC CCAGCACCAA TTCAAAACAG TGATGGGGAC 
GAGGAGGAAG ACTCGGGGGA AGACGATGTG GTGACCCAGG GGGACCTTTG GGGCTCTGAG 
GACAGTGATG AAGATATGGT GGATGACTAT GGAGCTGCCT CTAACTCGGA GGATGAGGAG 
GAAAAGCTGT TGCCTATTGA AAGAGCTGCC CTGAAGCAGA AAGCCCAGGA TGCCACAGCT 
GGGGTCCTGT GGAATGAAGA GGACACTGAT GAAGATGAAG ATGATGATGG TGTGTCCCCT 
GAGTCCCACC CCAGAAAGGA TGACAAAGCA GAGGGGGATT TGCAGATTAA TGTGGAAGAT 
GAGGAAGCCT TTGTGCTGCC CCCTGCTGGG GAGACGGACC AGGATGGCCA GGCTCCAGAC 
CTGCAGCGAG TTCACAAGCG GATCCAGGAC ATCGTGGGAG TGCTGCGGGA TTTCGGAGCT 
CAGCGAGAAG AGGGCCGGTC TCGAGCTGAG TATCTAAGTC GGCTTCAGAA GGACCTGGCC 
ACTTACTATT CTTATGGAGA CTTCCTGCTG AGCAAGCTCA TGGAGCTGTT CCCTCTGTCT 
GAGTTGATCG AGTTCTTAGA AGCTAATGAG GTGCCCCGGC CAATCACCCT TCGGACCAAC 
ACCTTGAAAA CCCGTCGCCG AGACCTTGCT CAGGCTCTGA TCAATCGTGG GGTTAATCTG 
GATCCACTGG GGAAGTGGTC AAAGTCTGGA CTTGTGGTAT ATGATTCTTC AGTGCCTATT 
GGCGCTACCC CTGAGTACCT CGCTGGACAC TATATGCTGC AGGGAGCTTC CAGTATGTTG 
CCCGTCATGG CCCTGGCACC TCAGGAGCAT GAGCGGATCT TAGACATGTG CTGTGCTCCT 
GGGGGGAAGA CCAGCTACAT AGCTCAGCTG ATGAAGAACA CAGGAGTGAT CCTTGCCAAC 
GATGCCAATG CAGACCGGCT CAAGAGCGTG GTGGGCAACC TGCACCGCTT GGGAGTCACC 
AACACCATCA TCAGCCACTA TGATGGGCGC CAGTTCCCCA AGGTGGTAGG GGGCTTTGAT 
CGAGTGCTAC TGGATGCTCC TTGTAGTGGC ACAGGAGTCA TCTCCAAAGA CCCTGCTGTG 
AAGACGAACA AGGATGAGAA AGACATCCAG CGCTGTGCCC ACCTTCAGAA GGAACTGCTG 
CTCAGTGCTA TCGACTCAGT GAACGCTGCC TCCAAGACCG GAGGCTACCT GGTCTACTGC 
ACCTGTTCCA TTACGGTGGA AGAGAACGAG TGGGTGGTAG ACTATGCCTT GAAGAAGAGG 
AATGTGCGGC TGGTGCCCAC CGGCCTGGAC TTCGGCCAGG AAGGTTTTAC CCGCTTCCAA 
GCAAGGCGCT TTCACCCCAC TCTGCGTTCC ACCCGGCGCT TCTACCCTCA CACTCACAAT 
ATGGACGGTT TCTTTATCGC CAAGTTCAAG AAATTTTCCA ATTCTATTCC CCAGCCCCAT 
GCAGGAAACT CGGCAGCAGC TACCCCTACA GAGCCTGACC TGAAGGATCA GGTCACCCCA 
AAGTCTGAGA ACGGTAGTCA GCCCACCAAG AAAGCCCGTG GGGCTGTGAA GGCAAAGCAG 
CAGTTGCTGA GACAGCCACA TTCCAAGAAG CCCTTCCAGA AGTTGAACGG CATCGCCAAA 
GGGCCAGGCT TGTCCACTGA ACCTTCTGTC CCAGATGCCC AAGTGTCTAC CAGGCCCAGT 
CAGTCTGCTG GGAATGCTGA CGTGAACAGC AAACGAAAGA GGTCTGAGAA GCTGAAGCAG 
CGGGGACCTA AGTGGAAGCC CTCTAAGGAG GCTGCTGTCC CAAAGCCGAG CGCCCCTTCC 
AGGGTTGAGG ACTCGAGAAC ACCTCCTGTG CCGACTCCCT CTGAAATCCG TGCTGCCCCA 
AGGCCTAAGG ACTGTGCTCC ATCCCTTGGG GAAGCCAAAA AGAAACAGAA AGGAAAGCAG 
CAGTTAGCAC AGCAGCCTGC TAATGGAGCT GCTCCCCTGA AAGAAGATGC TGTTTCCAAG 
GGACCCTCAG CCCCCTTTGT GTCTCCCCAC AGTTCCACCA GGCCCCCACC AGCAAAAAGG 
AGGAAATCAA TGACAAAGGG CAACAGCCAG CCTCTGTTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110957.1
CDS	127..2511
Translation	MGRKLDPTKKEKRGPGRKARKQKGAETELVRFLPAAGDENSKRLSSRARKRAAKRRAGSVDVPKPNKSPGIKTLPGELSKGAVQARGKKRPAPIQNSDGDEEEDSGEDDVVTQGDLWGSEDSDEDMVDDYGAASNSEDEEEKLLPIERAALKQKAQDATAGVLWNEEDTDEDEDDDGVSPESHPRKDDKAEGDLQINVEDEEAFVLPPAGETDQDGQAPDLQRVHKRIQDIVGVLRDFGAQREEGRSRAEYLSRLQKDLATYYSYGDFLLSKLMELFPLSELIEFLEANEVPRPITLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKSGLVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYIAQLMKNTGVILANDANADRLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDIQRCAHLQKELLLSAIDSVNAASKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFQARRFHPTLRSTRRFYPHTHNMDGFFIAKFKKFSNSIPQPHAGNSAAATPTEPDLKDQVTPKSENGSQPTKKARGAVKAKQQLLRQPHSKKPFQKLNGIAKGPGLSTEPSVPDAQVSTRPSQSAGNADVNSKRKRSEKLKQRGPKWKPSKEAAVPKPSAPSRVEDSRTPPVPTPSEIRAAPRPKDCAPSLGEAKKKQKGKQQLAQQPANGAAPLKEDAVSKGPSAPFVSPHSSTRPPPAKRRKSMTKGNSQPLLS

Target ORF information:

RefSeq Version	XM_030255097.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NOP2 nucleolar protein (Nop2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255097.1

ORF Insert Sequence:

ATGGGGCGCA AGTTGGATCC TACGAAGAAG GAGAAGCGTG GACCCGGCCG AAAGGCCCGA 
AAACAGAAGG GTGCGGAGAC CGAATTGGTC AGATTTTTGC CTGCAGCTGG CGATGAGAAT 
TCCAAGAGGC TGTCGAGTCG TGCCCGAAAG CGGGCAGCCA AGAGGAGGGC AGGGTCTGTT 
GACGTCCCTA AGCCAAATAA GTCTCCTGGG ATCAAAACAT TGCCTGGAGA GTTATCCAAA 
GGAGCAGTCC AGGCTCGAGG TAAGAAGCGC CCAGCACCAA TTCAAAACAG TGATGGGGAC 
GAGGAGGAAG ACTCGGGGGA AGACGATGTG GTGACCCAGG GGGACCTTTG GGGCTCTGAG 
GACAGTGATG AAGATATGGT GGATGACTAT GGAGCTGCCT CTAACTCGGA GGATGAGGAG 
GAAAAGCTGT TGCCTATTGA AAGAGCTGCC CTGAAGCAGA AAGCCCAGGA TGCCACAGCT 
GGGGTCCTGT GGAATGAAGA GGACACTGAT GAAGATGAAG ATGATGATGG TGTGTCCCCT 
GAGTCCCACC CCAGAAAGGA TGACAAAGCA GAGGGGGATT TGCAGATTAA TGTGGAAGAT 
GAGGAAGCCT TTGTGCTGCC CCCTGCTGGG GAGACGGACC AGGATGGCCA GGCTCCAGAC 
CTGCAGCGAG TTCACAAGCG GATCCAGGAC ATCGTGGGAG TGCTGCGGGA TTTCGGAGCT 
CAGCGAGAAG AGGGCCGGTC TCGAGCTGAG TATCTAAGTC GGCTTCAGAA GGACCTGGCC 
ACTTACTATT CTTATGGAGA CTTCCTGCTG AGCAAGCTCA TGGAGCTGTT CCCTCTGTCT 
GAGTTGATCG AGTTCTTAGA AGCTAATGAG GTGCCCCGGC CAATCACCCT TCGGACCAAC 
ACCTTGAAAA CCCGTCGCCG AGACCTTGCT CAGGCTCTGA TCAATCGTGG GGTTAATCTG 
GATCCACTGG GGAAGTGGTC AAAGTCTGGA CTTGTGGTAT ATGATTCTTC AGTGCCTATT 
GGCGCTACCC CTGAGTACCT CGCTGGACAC TATATGCTGC AGGGAGCTTC CAGTATGTTG 
CCCGTCATGG CCCTGGCACC TCAGGAGCAT GAGCGGATCT TAGACATGTG CTGTGCTCCT 
GGGGGGAAGA CCAGCTACAT AGCTCAGCTG ATGAAGAACA CAGGAGTGAT CCTTGCCAAC 
GATGCCAATG CAGACCGGCT CAAGAGCGTG GTGGGCAACC TGCACCGCTT GGGAGTCACC 
AACACCATCA TCAGCCACTA TGATGGGCGC CAGTTCCCCA AGGTGGTAGG GGGCTTTGAT 
CGAGTGCTAC TGGATGCTCC TTGTAGTGGC ACAGGAGTCA TCTCCAAAGA CCCTGCTGTG 
AAGACGAACA AGGATGAGAA AGACATCCAG CGCTGTGCCC ACCTTCAGAA GGAACTGCTG 
CTCAGTGCTA TCGACTCAGT GAACGCTGCC TCCAAGACCG GAGGCTACCT GGTCTACTGC 
ACCTGTTCCA TTACGGTGGA AGAGAACGAG TGGGTGGTAG ACTATGCCTT GAAGAAGAGG 
AATGTGCGGC TGGTGCCCAC CGGCCTGGAC TTCGGCCAGG AAGGTTTTAC CCGCTTCCAA 
GCAAGGCGCT TTCACCCCAC TCTGCGTTCC ACCCGGCGCT TCTACCCTCA CACTCACAAT 
ATGGACGGTT TCTTTATCGC CAAGTTCAAG AAATTTTCCA ATTCTATTCC CCAGCCCCAT 
GCAGGAAACT CGGCAGCAGC TACCCCTACA GAGCCTGACC TGAAGGATCA GGTCACCCCA 
AAGTCTGAGA ACGGTAGTCA GCCCACCAAG AAAGCCCGTG GGGCTGTGAA GGCAAAGCAG 
CAGTTGCTGA GACAGCCACA TTCCAAGAAG CCCTTCCAGA AGTTGAACGG CATCGCCAAA 
GGGCCAGGCT TGTCCACTGA ACCTTCTGTC CCAGATGCCC AAGTGTCTAC CAGGCCCAGT 
CAGTCTGCTG GGAATGCTGA CGTGAACAGC AAACGAAAGA GGTCTGAGAA GCTGAAGCAG 
CGGGGACCTA AGTGGAAGCC CTCTAAGGAG GCTGCTGTCC CAAAGCCGAG CGCCCCTTCC 
AGGGTTGAGG ACTCGAGAAC ACCTCCTGTG CCGACTCCCT CTGAAATCCG TGCTGCCCCA 
AGGCCTAAGG ACTGTGCTCC ATCCCTTGGG GAAGCCAAAA AGAAACAGAA AGGAAAGCAG 
CAGTTAGCAC AGCAGCCTGC TAATGGAGCT GCTCCCCTGA AAGAAGATGC TGTTTCCAAG 
GGACCCTCAG CCCCCTTTGT GTCTCCCCAC AGTTCCACCA GGCCCCCACC AGCAAAAAGG 
AGGAAATCAA TGACAAAGGG CAACAGCCAG CCTCTGTTGT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nop2 ( XM_017321322.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176811.1 Mus musculus NOP2 nucleolar protein (Nop2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81613
Clone ID Related Accession (Same CDS sequence)	XM_017321322.1 , XM_017321323.1
Accession Version	XM_017321322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGCA AGTTGGATCC TACGAAGAAG GAGAAGCGTG GACCCGGCCG AAAGGCCCGA 
AAACAGAAGG GTGCGGAGAC CGAATTGGTC AGATTTTTGC CTGCAGCTGG CGATGAGAAT 
TCCAAGAGGC TGTCGAGTCG TGCCCGAAAG CGGGCAGCCA AGAGGAGGGC AGGGTCTGTT 
GACGTCCCTA AGCCAAATAA GTCTCCTGGG ATCAAAACAT TGCCTGGAGA GTTATCCAAA 
GGAGCAGTCC AGGCTCGAGG TAAGAAGCGC CCAGCACCAA TTCAAAACAG TGATGGGGAC 
GAGGAGGAAG ACTCGGGGGA AGACGATGTG GTGACCCAGG GGGACCTTTG GGGCTCTGAG 
GACAGTGATG AAGATATGGT GGATGACTAT GGAGCTGCCT CTAACTCGGA GGATGAGGAG 
GAAAAGCTGT TGCCTATTGA AAGAGCTGCC CTGAAGCAGA AAGCCCAGGA TGCCACAGCT 
GGGGTCCTGT GGAATGAAGA GGACACTGAT GAAGATGAAG ATGATGATGG TGTGTCCCCT 
GAGTCCCACC CCAGAAAGGA TGACAAAGCA GAGGGGGATT TGCAGATTAA TGTGGAAGAT 
GAGGAAGCCT TTGTGCTGCC CCCTGCTGGG GAGACGGACC AGGATGGCCA GGCTCCAGAC 
CTGCAGCGAG TTCACAAGCG GATCCAGGAC ATCGTGGGAG TGCTGCGGGA TTTCGGAGCT 
CAGCGAGAAG AGGGCCGGTC TCGAGCTGAG TATCTAAGTC GGCTTCAGAA GGACCTGGCC 
ACTTACTATT CTTATGGAGA CTTCCTGCTG AGCAAGCTCA TGGAGCTGTT CCCTCTGTCT 
GAGTTGATCG AGTTCTTAGA AGCTAATGAG GTGCCCCGGC CAATCACCCT TCGGACCAAC 
ACCTTGAAAA CCCGTCGCCG AGACCTTGCT CAGGCTCTGA TCAATCGTGG GGTTAATCTG 
GATCCACTGG GGAAGTGGTC AAAGTCTGGA CTTGTGGTAT ATGATTCTTC AGTGCCTATT 
GGTAAGTGTC CCCAGCTGGT AGCTAGCCCT TCTGTCCCTT CCCTCTGCCA GCATGTCACA 
CCATGGCCCT CCTTCACAGG CGCTACCCCT GAGTACCTCG CTGGACACTA TATGCTGCAG 
GGAGCTTCCA GTATGTTGCC CGTCATGGCC CTGGCACCTC AGGAGCATGA GCGGATCTTA 
GACATGTGCT GTGCTCCTGG GGGGAAGACC AGCTACATAG CTCAGCTGAT GAAGAACACA 
GGAGTGATCC TTGCCAACGA TGCCAATGCA GACCGGCTCA AGAGCGTGGT GGGCAACCTG 
CACCGCTTGG GAGTCACCAA CACCATCATC AGCCACTATG ATGGGCGCCA GTTCCCCAAG 
GTGGTAGGGG GCTTTGATCG AGTGCTACTG GATGCTCCTT GTAGTGGCAC AGGAGTCATC 
TCCAAAGACC CTGCTGTGAA GACGAACAAG GATGAGAAAG ACATCCAGCG CTGTGCCCAC 
CTTCAGAAGG AACTGCTGCT CAGTGCTATC GACTCAGTGA ACGCTGCCTC CAAGACCGGA 
GGCTACCTGG TCTACTGCAC CTGTTCCATT ACGGTGGAAG AGAACGAGTG GGTGGTAGAC 
TATGCCTTGA AGAAGAGGAA TGTGCGGCTG GTGCCCACCG GCCTGGACTT CGGCCAGGAA 
GGTTTTACCC GCTTCCAAGC AAGGCGCTTT CACCCCACTC TGCGTTCCAC CCGGCGCTTC 
TACCCTCACA CTCACAATAT GGACGGTTTC TTTATCGCCA AGTTCAAGAA ATTTTCCAAT 
TCTATTCCCC AGCCCCATGC AGGAAACTCG GCAGCAGCTA CCCCTACAGA GCCTGACCTG 
AAGGATCAGG TCACCCCAAA GTCTGAGAAC GGTAGTCAGC CCACCAAGAA AGCCCGTGGG 
GCTGTGAAGG CAAAGCAGCA GTTGCTGAGA CAGCCACATT CCAAGAAGCC CTTCCAGAAG 
TTGAACGGCA TCGCCAAAGG GCCAGGCTTG TCCACTGAAC CTTCTGTCCC AGATGCCCAA 
GTGTCTACCA GGCCCAGTCA GTCTGCTGGG AATGCTGACG TGAACAGCAA ACGAAAGAGG 
TCTGAGAAGC TGAAGCAGCG GGGACCTAAG TGGAAGCCCT CTAAGGAGGC TGCTGTCCCA 
AAGCCGAGCG CCCCTTCCAG GGTTGAGGAC TCGAGAACAC CTCCTGTGCC GACTCCCTCT 
GAAATCCGTG CTGCCCCAAG GCCTAAGGAC TGTGCTCCAT CCCTTGGGGA AGCCAAAAAG 
AAACAGAAAG GAAAGCAGCA GTTAGCACAG CAGCCTGCTA ATGGAGCTGC TCCCCTGAAA 
GAAGATGCTG TTTCCAAGGG ACCCTCAGCC CCCTTTGTGT CTCCCCACAG TTCCACCAGG 
CCCCCACCAG CAAAAAGGAG GAAATCAATG ACAAAGGGCA ACAGCCAGCC TCTGTTGTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176811.1
CDS	146..2608
Translation	MGRKLDPTKKEKRGPGRKARKQKGAETELVRFLPAAGDENSKRLSSRARKRAAKRRAGSVDVPKPNKSPGIKTLPGELSKGAVQARGKKRPAPIQNSDGDEEEDSGEDDVVTQGDLWGSEDSDEDMVDDYGAASNSEDEEEKLLPIERAALKQKAQDATAGVLWNEEDTDEDEDDDGVSPESHPRKDDKAEGDLQINVEDEEAFVLPPAGETDQDGQAPDLQRVHKRIQDIVGVLRDFGAQREEGRSRAEYLSRLQKDLATYYSYGDFLLSKLMELFPLSELIEFLEANEVPRPITLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKSGLVVYDSSVPIGKCPQLVASPSVPSLCQHVTPWPSFTGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYIAQLMKNTGVILANDANADRLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDIQRCAHLQKELLLSAIDSVNAASKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFQARRFHPTLRSTRRFYPHTHNMDGFFIAKFKKFSNSIPQPHAGNSAAATPTEPDLKDQVTPKSENGSQPTKKARGAVKAKQQLLRQPHSKKPFQKLNGIAKGPGLSTEPSVPDAQVSTRPSQSAGNADVNSKRKRSEKLKQRGPKWKPSKEAAVPKPSAPSRVEDSRTPPVPTPSEIRAAPRPKDCAPSLGEAKKKQKGKQQLAQQPANGAAPLKEDAVSKGPSAPFVSPHSSTRPPPAKRRKSMTKGNSQPLLS

Target ORF information:

RefSeq Version	XM_017321322.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NOP2 nucleolar protein (Nop2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321322.1

ORF Insert Sequence:

ATGGGGCGCA AGTTGGATCC TACGAAGAAG GAGAAGCGTG GACCCGGCCG AAAGGCCCGA 
AAACAGAAGG GTGCGGAGAC CGAATTGGTC AGATTTTTGC CTGCAGCTGG CGATGAGAAT 
TCCAAGAGGC TGTCGAGTCG TGCCCGAAAG CGGGCAGCCA AGAGGAGGGC AGGGTCTGTT 
GACGTCCCTA AGCCAAATAA GTCTCCTGGG ATCAAAACAT TGCCTGGAGA GTTATCCAAA 
GGAGCAGTCC AGGCTCGAGG TAAGAAGCGC CCAGCACCAA TTCAAAACAG TGATGGGGAC 
GAGGAGGAAG ACTCGGGGGA AGACGATGTG GTGACCCAGG GGGACCTTTG GGGCTCTGAG 
GACAGTGATG AAGATATGGT GGATGACTAT GGAGCTGCCT CTAACTCGGA GGATGAGGAG 
GAAAAGCTGT TGCCTATTGA AAGAGCTGCC CTGAAGCAGA AAGCCCAGGA TGCCACAGCT 
GGGGTCCTGT GGAATGAAGA GGACACTGAT GAAGATGAAG ATGATGATGG TGTGTCCCCT 
GAGTCCCACC CCAGAAAGGA TGACAAAGCA GAGGGGGATT TGCAGATTAA TGTGGAAGAT 
GAGGAAGCCT TTGTGCTGCC CCCTGCTGGG GAGACGGACC AGGATGGCCA GGCTCCAGAC 
CTGCAGCGAG TTCACAAGCG GATCCAGGAC ATCGTGGGAG TGCTGCGGGA TTTCGGAGCT 
CAGCGAGAAG AGGGCCGGTC TCGAGCTGAG TATCTAAGTC GGCTTCAGAA GGACCTGGCC 
ACTTACTATT CTTATGGAGA CTTCCTGCTG AGCAAGCTCA TGGAGCTGTT CCCTCTGTCT 
GAGTTGATCG AGTTCTTAGA AGCTAATGAG GTGCCCCGGC CAATCACCCT TCGGACCAAC 
ACCTTGAAAA CCCGTCGCCG AGACCTTGCT CAGGCTCTGA TCAATCGTGG GGTTAATCTG 
GATCCACTGG GGAAGTGGTC AAAGTCTGGA CTTGTGGTAT ATGATTCTTC AGTGCCTATT 
GGTAAGTGTC CCCAGCTGGT AGCTAGCCCT TCTGTCCCTT CCCTCTGCCA GCATGTCACA 
CCATGGCCCT CCTTCACAGG CGCTACCCCT GAGTACCTCG CTGGACACTA TATGCTGCAG 
GGAGCTTCCA GTATGTTGCC CGTCATGGCC CTGGCACCTC AGGAGCATGA GCGGATCTTA 
GACATGTGCT GTGCTCCTGG GGGGAAGACC AGCTACATAG CTCAGCTGAT GAAGAACACA 
GGAGTGATCC TTGCCAACGA TGCCAATGCA GACCGGCTCA AGAGCGTGGT GGGCAACCTG 
CACCGCTTGG GAGTCACCAA CACCATCATC AGCCACTATG ATGGGCGCCA GTTCCCCAAG 
GTGGTAGGGG GCTTTGATCG AGTGCTACTG GATGCTCCTT GTAGTGGCAC AGGAGTCATC 
TCCAAAGACC CTGCTGTGAA GACGAACAAG GATGAGAAAG ACATCCAGCG CTGTGCCCAC 
CTTCAGAAGG AACTGCTGCT CAGTGCTATC GACTCAGTGA ACGCTGCCTC CAAGACCGGA 
GGCTACCTGG TCTACTGCAC CTGTTCCATT ACGGTGGAAG AGAACGAGTG GGTGGTAGAC 
TATGCCTTGA AGAAGAGGAA TGTGCGGCTG GTGCCCACCG GCCTGGACTT CGGCCAGGAA 
GGTTTTACCC GCTTCCAAGC AAGGCGCTTT CACCCCACTC TGCGTTCCAC CCGGCGCTTC 
TACCCTCACA CTCACAATAT GGACGGTTTC TTTATCGCCA AGTTCAAGAA ATTTTCCAAT 
TCTATTCCCC AGCCCCATGC AGGAAACTCG GCAGCAGCTA CCCCTACAGA GCCTGACCTG 
AAGGATCAGG TCACCCCAAA GTCTGAGAAC GGTAGTCAGC CCACCAAGAA AGCCCGTGGG 
GCTGTGAAGG CAAAGCAGCA GTTGCTGAGA CAGCCACATT CCAAGAAGCC CTTCCAGAAG 
TTGAACGGCA TCGCCAAAGG GCCAGGCTTG TCCACTGAAC CTTCTGTCCC AGATGCCCAA 
GTGTCTACCA GGCCCAGTCA GTCTGCTGGG AATGCTGACG TGAACAGCAA ACGAAAGAGG 
TCTGAGAAGC TGAAGCAGCG GGGACCTAAG TGGAAGCCCT CTAAGGAGGC TGCTGTCCCA 
AAGCCGAGCG CCCCTTCCAG GGTTGAGGAC TCGAGAACAC CTCCTGTGCC GACTCCCTCT 
GAAATCCGTG CTGCCCCAAG GCCTAAGGAC TGTGCTCCAT CCCTTGGGGA AGCCAAAAAG 
AAACAGAAAG GAAAGCAGCA GTTAGCACAG CAGCCTGCTA ATGGAGCTGC TCCCCTGAAA 
GAAGATGCTG TTTCCAAGGG ACCCTCAGCC CCCTTTGTGT CTCCCCACAG TTCCACCAGG 
CCCCCACCAG CAAAAAGGAG GAAATCAATG ACAAAGGGCA ACAGCCAGCC TCTGTTGTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nop2 ( XM_017321323.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176812.1 Mus musculus NOP2 nucleolar protein (Nop2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu81613
Clone ID Related Accession (Same CDS sequence)	XM_017321322.1 , XM_017321323.1
Accession Version	XM_017321323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable 28S rRNA (cytosine-C(5))-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGCA AGTTGGATCC TACGAAGAAG GAGAAGCGTG GACCCGGCCG AAAGGCCCGA 
AAACAGAAGG GTGCGGAGAC CGAATTGGTC AGATTTTTGC CTGCAGCTGG CGATGAGAAT 
TCCAAGAGGC TGTCGAGTCG TGCCCGAAAG CGGGCAGCCA AGAGGAGGGC AGGGTCTGTT 
GACGTCCCTA AGCCAAATAA GTCTCCTGGG ATCAAAACAT TGCCTGGAGA GTTATCCAAA 
GGAGCAGTCC AGGCTCGAGG TAAGAAGCGC CCAGCACCAA TTCAAAACAG TGATGGGGAC 
GAGGAGGAAG ACTCGGGGGA AGACGATGTG GTGACCCAGG GGGACCTTTG GGGCTCTGAG 
GACAGTGATG AAGATATGGT GGATGACTAT GGAGCTGCCT CTAACTCGGA GGATGAGGAG 
GAAAAGCTGT TGCCTATTGA AAGAGCTGCC CTGAAGCAGA AAGCCCAGGA TGCCACAGCT 
GGGGTCCTGT GGAATGAAGA GGACACTGAT GAAGATGAAG ATGATGATGG TGTGTCCCCT 
GAGTCCCACC CCAGAAAGGA TGACAAAGCA GAGGGGGATT TGCAGATTAA TGTGGAAGAT 
GAGGAAGCCT TTGTGCTGCC CCCTGCTGGG GAGACGGACC AGGATGGCCA GGCTCCAGAC 
CTGCAGCGAG TTCACAAGCG GATCCAGGAC ATCGTGGGAG TGCTGCGGGA TTTCGGAGCT 
CAGCGAGAAG AGGGCCGGTC TCGAGCTGAG TATCTAAGTC GGCTTCAGAA GGACCTGGCC 
ACTTACTATT CTTATGGAGA CTTCCTGCTG AGCAAGCTCA TGGAGCTGTT CCCTCTGTCT 
GAGTTGATCG AGTTCTTAGA AGCTAATGAG GTGCCCCGGC CAATCACCCT TCGGACCAAC 
ACCTTGAAAA CCCGTCGCCG AGACCTTGCT CAGGCTCTGA TCAATCGTGG GGTTAATCTG 
GATCCACTGG GGAAGTGGTC AAAGTCTGGA CTTGTGGTAT ATGATTCTTC AGTGCCTATT 
GGTAAGTGTC CCCAGCTGGT AGCTAGCCCT TCTGTCCCTT CCCTCTGCCA GCATGTCACA 
CCATGGCCCT CCTTCACAGG CGCTACCCCT GAGTACCTCG CTGGACACTA TATGCTGCAG 
GGAGCTTCCA GTATGTTGCC CGTCATGGCC CTGGCACCTC AGGAGCATGA GCGGATCTTA 
GACATGTGCT GTGCTCCTGG GGGGAAGACC AGCTACATAG CTCAGCTGAT GAAGAACACA 
GGAGTGATCC TTGCCAACGA TGCCAATGCA GACCGGCTCA AGAGCGTGGT GGGCAACCTG 
CACCGCTTGG GAGTCACCAA CACCATCATC AGCCACTATG ATGGGCGCCA GTTCCCCAAG 
GTGGTAGGGG GCTTTGATCG AGTGCTACTG GATGCTCCTT GTAGTGGCAC AGGAGTCATC 
TCCAAAGACC CTGCTGTGAA GACGAACAAG GATGAGAAAG ACATCCAGCG CTGTGCCCAC 
CTTCAGAAGG AACTGCTGCT CAGTGCTATC GACTCAGTGA ACGCTGCCTC CAAGACCGGA 
GGCTACCTGG TCTACTGCAC CTGTTCCATT ACGGTGGAAG AGAACGAGTG GGTGGTAGAC 
TATGCCTTGA AGAAGAGGAA TGTGCGGCTG GTGCCCACCG GCCTGGACTT CGGCCAGGAA 
GGTTTTACCC GCTTCCAAGC AAGGCGCTTT CACCCCACTC TGCGTTCCAC CCGGCGCTTC 
TACCCTCACA CTCACAATAT GGACGGTTTC TTTATCGCCA AGTTCAAGAA ATTTTCCAAT 
TCTATTCCCC AGCCCCATGC AGGAAACTCG GCAGCAGCTA CCCCTACAGA GCCTGACCTG 
AAGGATCAGG TCACCCCAAA GTCTGAGAAC GGTAGTCAGC CCACCAAGAA AGCCCGTGGG 
GCTGTGAAGG CAAAGCAGCA GTTGCTGAGA CAGCCACATT CCAAGAAGCC CTTCCAGAAG 
TTGAACGGCA TCGCCAAAGG GCCAGGCTTG TCCACTGAAC CTTCTGTCCC AGATGCCCAA 
GTGTCTACCA GGCCCAGTCA GTCTGCTGGG AATGCTGACG TGAACAGCAA ACGAAAGAGG 
TCTGAGAAGC TGAAGCAGCG GGGACCTAAG TGGAAGCCCT CTAAGGAGGC TGCTGTCCCA 
AAGCCGAGCG CCCCTTCCAG GGTTGAGGAC TCGAGAACAC CTCCTGTGCC GACTCCCTCT 
GAAATCCGTG CTGCCCCAAG GCCTAAGGAC TGTGCTCCAT CCCTTGGGGA AGCCAAAAAG 
AAACAGAAAG GAAAGCAGCA GTTAGCACAG CAGCCTGCTA ATGGAGCTGC TCCCCTGAAA 
GAAGATGCTG TTTCCAAGGG ACCCTCAGCC CCCTTTGTGT CTCCCCACAG TTCCACCAGG 
CCCCCACCAG CAAAAAGGAG GAAATCAATG ACAAAGGGCA ACAGCCAGCC TCTGTTGTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176812.1
CDS	172..2634
Translation	MGRKLDPTKKEKRGPGRKARKQKGAETELVRFLPAAGDENSKRLSSRARKRAAKRRAGSVDVPKPNKSPGIKTLPGELSKGAVQARGKKRPAPIQNSDGDEEEDSGEDDVVTQGDLWGSEDSDEDMVDDYGAASNSEDEEEKLLPIERAALKQKAQDATAGVLWNEEDTDEDEDDDGVSPESHPRKDDKAEGDLQINVEDEEAFVLPPAGETDQDGQAPDLQRVHKRIQDIVGVLRDFGAQREEGRSRAEYLSRLQKDLATYYSYGDFLLSKLMELFPLSELIEFLEANEVPRPITLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKSGLVVYDSSVPIGKCPQLVASPSVPSLCQHVTPWPSFTGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYIAQLMKNTGVILANDANADRLKSVVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDIQRCAHLQKELLLSAIDSVNAASKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFQARRFHPTLRSTRRFYPHTHNMDGFFIAKFKKFSNSIPQPHAGNSAAATPTEPDLKDQVTPKSENGSQPTKKARGAVKAKQQLLRQPHSKKPFQKLNGIAKGPGLSTEPSVPDAQVSTRPSQSAGNADVNSKRKRSEKLKQRGPKWKPSKEAAVPKPSAPSRVEDSRTPPVPTPSEIRAAPRPKDCAPSLGEAKKKQKGKQQLAQQPANGAAPLKEDAVSKGPSAPFVSPHSSTRPPPAKRRKSMTKGNSQPLLS

Target ORF information:

RefSeq Version	XM_017321323.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NOP2 nucleolar protein (Nop2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321323.1

ORF Insert Sequence:

ATGGGGCGCA AGTTGGATCC TACGAAGAAG GAGAAGCGTG GACCCGGCCG AAAGGCCCGA 
AAACAGAAGG GTGCGGAGAC CGAATTGGTC AGATTTTTGC CTGCAGCTGG CGATGAGAAT 
TCCAAGAGGC TGTCGAGTCG TGCCCGAAAG CGGGCAGCCA AGAGGAGGGC AGGGTCTGTT 
GACGTCCCTA AGCCAAATAA GTCTCCTGGG ATCAAAACAT TGCCTGGAGA GTTATCCAAA 
GGAGCAGTCC AGGCTCGAGG TAAGAAGCGC CCAGCACCAA TTCAAAACAG TGATGGGGAC 
GAGGAGGAAG ACTCGGGGGA AGACGATGTG GTGACCCAGG GGGACCTTTG GGGCTCTGAG 
GACAGTGATG AAGATATGGT GGATGACTAT GGAGCTGCCT CTAACTCGGA GGATGAGGAG 
GAAAAGCTGT TGCCTATTGA AAGAGCTGCC CTGAAGCAGA AAGCCCAGGA TGCCACAGCT 
GGGGTCCTGT GGAATGAAGA GGACACTGAT GAAGATGAAG ATGATGATGG TGTGTCCCCT 
GAGTCCCACC CCAGAAAGGA TGACAAAGCA GAGGGGGATT TGCAGATTAA TGTGGAAGAT 
GAGGAAGCCT TTGTGCTGCC CCCTGCTGGG GAGACGGACC AGGATGGCCA GGCTCCAGAC 
CTGCAGCGAG TTCACAAGCG GATCCAGGAC ATCGTGGGAG TGCTGCGGGA TTTCGGAGCT 
CAGCGAGAAG AGGGCCGGTC TCGAGCTGAG TATCTAAGTC GGCTTCAGAA GGACCTGGCC 
ACTTACTATT CTTATGGAGA CTTCCTGCTG AGCAAGCTCA TGGAGCTGTT CCCTCTGTCT 
GAGTTGATCG AGTTCTTAGA AGCTAATGAG GTGCCCCGGC CAATCACCCT TCGGACCAAC 
ACCTTGAAAA CCCGTCGCCG AGACCTTGCT CAGGCTCTGA TCAATCGTGG GGTTAATCTG 
GATCCACTGG GGAAGTGGTC AAAGTCTGGA CTTGTGGTAT ATGATTCTTC AGTGCCTATT 
GGTAAGTGTC CCCAGCTGGT AGCTAGCCCT TCTGTCCCTT CCCTCTGCCA GCATGTCACA 
CCATGGCCCT CCTTCACAGG CGCTACCCCT GAGTACCTCG CTGGACACTA TATGCTGCAG 
GGAGCTTCCA GTATGTTGCC CGTCATGGCC CTGGCACCTC AGGAGCATGA GCGGATCTTA 
GACATGTGCT GTGCTCCTGG GGGGAAGACC AGCTACATAG CTCAGCTGAT GAAGAACACA 
GGAGTGATCC TTGCCAACGA TGCCAATGCA GACCGGCTCA AGAGCGTGGT GGGCAACCTG 
CACCGCTTGG GAGTCACCAA CACCATCATC AGCCACTATG ATGGGCGCCA GTTCCCCAAG 
GTGGTAGGGG GCTTTGATCG AGTGCTACTG GATGCTCCTT GTAGTGGCAC AGGAGTCATC 
TCCAAAGACC CTGCTGTGAA GACGAACAAG GATGAGAAAG ACATCCAGCG CTGTGCCCAC 
CTTCAGAAGG AACTGCTGCT CAGTGCTATC GACTCAGTGA ACGCTGCCTC CAAGACCGGA 
GGCTACCTGG TCTACTGCAC CTGTTCCATT ACGGTGGAAG AGAACGAGTG GGTGGTAGAC 
TATGCCTTGA AGAAGAGGAA TGTGCGGCTG GTGCCCACCG GCCTGGACTT CGGCCAGGAA 
GGTTTTACCC GCTTCCAAGC AAGGCGCTTT CACCCCACTC TGCGTTCCAC CCGGCGCTTC 
TACCCTCACA CTCACAATAT GGACGGTTTC TTTATCGCCA AGTTCAAGAA ATTTTCCAAT 
TCTATTCCCC AGCCCCATGC AGGAAACTCG GCAGCAGCTA CCCCTACAGA GCCTGACCTG 
AAGGATCAGG TCACCCCAAA GTCTGAGAAC GGTAGTCAGC CCACCAAGAA AGCCCGTGGG 
GCTGTGAAGG CAAAGCAGCA GTTGCTGAGA CAGCCACATT CCAAGAAGCC CTTCCAGAAG 
TTGAACGGCA TCGCCAAAGG GCCAGGCTTG TCCACTGAAC CTTCTGTCCC AGATGCCCAA 
GTGTCTACCA GGCCCAGTCA GTCTGCTGGG AATGCTGACG TGAACAGCAA ACGAAAGAGG 
TCTGAGAAGC TGAAGCAGCG GGGACCTAAG TGGAAGCCCT CTAAGGAGGC TGCTGTCCCA 
AAGCCGAGCG CCCCTTCCAG GGTTGAGGAC TCGAGAACAC CTCCTGTGCC GACTCCCTCT 
GAAATCCGTG CTGCCCCAAG GCCTAAGGAC TGTGCTCCAT CCCTTGGGGA AGCCAAAAAG 
AAACAGAAAG GAAAGCAGCA GTTAGCACAG CAGCCTGCTA ATGGAGCTGC TCCCCTGAAA 
GAAGATGCTG TTTCCAAGGG ACCCTCAGCC CCCTTTGTGT CTCCCCACAG TTCCACCAGG 
CCCCCACCAG CAAAAAGGAG GAAATCAATG ACAAAGGGCA ACAGCCAGCC TCTGTTGTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Scientific reports637396(2016 Nov)
Cui W,Dai X,Marcho C,Han Z,Zhang K,Tremblay KD,Mager J

Nop2 is required for mammalian preimplantation development.
Molecular reproduction and development83(2)124-31(2016 Feb)
Cui W,Pizzollo J,Han Z,Marcho C,Zhang K,Mager J

Nop2 is expressed during proliferation of neural stem cells and in adult mouse and human brain.
Brain research159765-76(2015 Feb)
Kosi N,Ali? I,Kola?evi? M,Vrsaljko N,Jovanov Milo?evi? N,Sobol M,Philimonenko A,Hoz?k P,Gajovi? S,Pochet R,Mitre?i? D

The AERO system: a 3D-like approach for recording gene expression patterns in the whole mouse embryo.
PloS one8(10)e75754(2013)
Shimizu H,Kubo A,Uchibe K,Hashimoto M,Yokoyama S,Takada S,Mitsuoka K,Asahara H

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

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Nop2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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