Pygl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pygl
Entrez Gene ID	110095
Full Name	liver glycogen phosphorylase
General protein information	Preferred Names liver glycogen phosphorylase Names glycogen phosphorylase, liver form
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 C2\|12 29.01 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_133198.2	NP_573461.2	glycogen phosphorylase, liver form

Pathways from BioSystems

Homologies

Homo sapiens (human)	PYGL	NP_002854.3
Gallus gallus (chicken)	PYGL	NP_989723.1
Pan troglodytes (chimpanzee)	PYGL	XP_001145433.2
Mus musculus (house mouse)	Pygl	NP_573461.2
Saccharomyces cerevisiae (baker's yeast)	GPH1	NP_015486.1
Eremothecium gossypii	AGOS_AFR547W	NP_986094.1
Arabidopsis thaliana (thale cress)	AT3G29320	NP_189578.1
Magnaporthe oryzae (rice blast fungus)	MGG_01819	XP_003714833.1
Neurospora crassa	NCU07027	XP_962166.2
	PYGL	XP_001102253.1
Xenopus tropicalis (tropical clawed frog)	pygl	XP_002936619.1
Canis lupus familiaris (dog)	PYGL	XP_003639242.1
Bos taurus (cattle)	PYGL	NP_001068671.1
Rattus norvegicus (Norway rat)	Pygl	NP_071604.1
Kluyveromyces lactis	KLLA0F10065g	XP_455538.1

Gene Ontology
Bibliography

Related articles in PubMed

Hepatic Glucocorticoid Receptor Plays a Greater Role Than Adipose GR in Metabolic Syndrome Despite Renal Compensation.
Bose SK, Hutson I, Harris CA
Endocrinology157(12)4943-4960(2016 Dec)

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
Wu Y, Wei FY, Kawarada L, Suzuki T, Araki K, Komohara Y, Fujimura A, Kaitsuka T, Takeya M, Oike Y, Suzuki T, Tomizawa K
PLoS genetics12(9)e1006355(2016 Sep)

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Gurdziel K, Vogt KR, Walton KD, Schneider GK, Gumucio DL
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)

Diabetes risk gene and Wnt effector Tcf7l2/TCF4 controls hepatic response to perinatal and adult metabolic demand.
Boj SF, van Es JH, Huch M, Li VS, Jos? A, Hatzis P, Mokry M, Haegebarth A, van den Born M, Chambon P, Voshol P, Dor Y, Cuppen E, Fillat C, Clevers H
Cell151(7)1595-607(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The in vivo consequences of disrupting the G(L)-phosphorylase a interaction, using a mouse model containing a Tyr284Phe substitution in the phosphorylase a-binding region of the hepatic glycogen-associated protein phosphatase-1 protein, is reported.
Title: Disruption of the allosteric phosphorylase a regulation of the hepatic glycogen-targeted protein phosphatase 1 improves glucose tolerance in vivo.

The following Pygl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pygl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15408	NM_133198.2 Latest version!	Mus musculus liver glycogen phosphorylase (Pygl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pygl ( NM_133198.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_573461.2 Mus musculus liver glycogen phosphorylase (Pygl), mRNA.

Price & Availability↑

CloneID	OMu15408
Clone ID Related Accession (Same CDS sequence)	NM_133198.2
Accession Version	NM_133198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	glycogen phosphorylase, liver form
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK145989.1 and BY435276.1. On Nov 19, 2009 this sequence version replaced NM_133198.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145989.1, AK167337.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAAGC CCCTGACCGA CCAGGAGAAG CGACGGCAGA TCAGCATCCG AGGCATCGTG 
GGCGTAGAGA ATGTGGCCGA GCTGAAAAAG GGTTTCAACC GTCACCTGCA CTTCACTCTG 
GTCAAGGACC GCAATGTGGC CACCCCCCGC GACTACTACT TCGCCCTTGC GCACACAGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGT ACACAGCAGC ACTACTACGA CAAGTGTCCC 
AAGAGGGTGT ATTACCTCTC TCTGGAATTT TACATGGGCC GAACATTACA GAACACCATG 
ATCAACCTTG GCTTACAAAA TGCCTGCGAT GAGGCCATTT ACCAGCTTGG ATTGGACATG 
GAAGAGTTAG AAGAAATTGA AGAAGATGCC GGCCTTGGCA ATGGCGGTCT TGGGAGGCTT 
GCTGCCTGCT TCCTGGACTC CATGGCAACC CTGGGACTTG CAGCCTATGG CTACGGCATT 
CGTTATGAAT ACGGAATCTT CAATCAGAAG ATCCGAGAGG GATGGCAGGT AGAAGAGGCA 
GATGACTGGC TCAGGCATGG AAACCCTTGG GAGAAGGCTC GCCCTGAATT CATGCTGCCC 
GTGCATTTCT ACGGAAGAGT AGAGCACACC CAGACGGGGA CAAAGTGGGT CGACACCCAG 
GTGGTCCTGG CTCTGCCTTA CGACACCCCC GTGCCTGGAT ATATGAACAA CACTGTGAAC 
ACTATGCGCC TCTGGTCGGC TCGAGCACCA AATGACTTTA ACCTTCAAGA TTTTAATGTT 
GGAGACTACA TTCAGGCTGT GCTGGACCGG AACCTGGCTG AGAATATCTC CAGAGTGCTC 
TACCCCAATG ATAACTTCTT TGAAGGGAAG GAGTTGCGGC TGAAACAGGA GTACTTTGTG 
GTGGCTGCCA CCCTGCAGGA TGTCATCCGG CGCTTCAAGG CCTCCAAGTT CGGCTCCAAG 
GATGGCATGG GAACCGTGTT TGATGCCTTT CCAGATCAGG TAGCCATCCA GCTGAATGAC 
ACACATCCTG CACTCGCCAT TCCAGAGCTG ATGAGGATTT TTGTGGACAT TGAAAAACTG 
CCCTGGGCCA AGGCATGGGA GATCACGAAG AAGACCTTCG CCTACACCAA CCACACGGTG 
CTCCCGGAGG CCCTGGAGCG CTGGCCGGTG GAACTGGTGG AGAAGCTGCT GCCTCGACAC 
TTGGAGATCA TTTATGAGAT CAATCAGAAA CATTTAGACA GAATTGTGGC CTTGTTTCCT 
AAAGACATCA GCCGCATGCG GAGAATGTCT CTCATTGAGG AGGAAGGAGG CAAACGGATC 
AACATGGCCC ACCTCTGCAT CGTGGGCTGC CACGCGGTGA ACGGTGTAGC AAAGATCCAC 
TCGGACATCG TGAAGACCCA AGTATTCAAG GACTTCAGCG AGCTAGAACC AGACAAGTTC 
CAGAATAAAA CCAACGGGAT TACCCCGAGG CGCTGGCTCC TACTCTGCAA CCCAGGGCTG 
GCTGACTTGA TAGCGGAGAA AATTGGAGAG GATTATGTGA AAGACCTGAG CCAGCTGACG 
AAGCTCCACA GTTTTGTGAG TGATGACATC TTCCTCCGGG AAATAGCCAA AGTGAAACAG 
GAAAATAAGC TGAAATTCTC CCAGTTCCTG GAGAAGGAAT ACAAGGTGAA GATCAACCCA 
TCCTCCATGT TTGATGTCCA TGTGAAGCGG ATCCACGAGT ATAAAAGGCA GCTTCTGAAC 
TGCCTGCATG TGATCACCAT GTACAATCGC ATCAAGAAAG ACCCTAAGAA ATTCTTCGTG 
CCAAGGACAG TCATAATTGG TGGCAAAGCT GCCCCAGGAT ATCACATGGC CAAAATGATC 
ATAAAGCTGA TCACCTCTGT GGCAGAAGTG GTGAACAATG ACCCCATGGT CGGCAGCAAG 
TTGAAAGTCA TCTTCTTGGA GAACTACAGA GTGTCTCTTG CCGAAAAAGT CATTCCAGCC 
ACAGACCTAT CGGAGCAGAT CTCCACGGCA GGCACGGAAG CCTCCGGGAC AGGCAACATG 
AAGTTCATGC TGAACGGGGC CCTGACCATC GGGACTATGG ATGGGGCCAA TGTGGAGATG 
GCAGAGGAAG CTGGGGAGGA GAACCTGTTC ATCTTTGGCA TGAGAGTAGA TGATGTGGCT 
GCTTTGGATA AGAAGGGGTA TGAGGCCAAA GAATATTACG AGGCCCTTCC AGAACTGAAG 
TTGGTCATCG ACCAAATCGA CAATGGCTTC TTTTCTCCCA ATCAGCCAGA CCTCTTCAAA 
GACATCATCA ACATGTTATT TTATCATGAC AGATTTAAAG TCTTTGCAGA CTACGAAGCC 
TATGTCAAGT GTCAAGAAAA AGTCAGTCAG CTGTATATGA ATCAAAAAGC CTGGAACACA 
ATGGTACTCA AAAACATAGC TGCCTCAGGG AAGTTCTCCA GTGACCGAAC AATTAAGGAG 
TATGCCAAGG ACATCTGGAA CATGGAGCCT TCGGATCTGA AGATTTCCCT ATCCAACGAG 
TCCAGCAATG GGGTCAGTGC CAATGGGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573461.2
CDS	101..2653
Translation	MAKPLTDQEKRRQISIRGIVGVENVAELKKGFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIREGWQVEEADDWLRHGNPWEKARPEFMLPVHFYGRVEHTQTGTKWVDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLQDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDVIRRFKASKFGSKDGMGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWAKAWEITKKTFAYTNHTVLPEALERWPVELVEKLLPRHLEIIYEINQKHLDRIVALFPKDISRMRRMSLIEEEGGKRINMAHLCIVGCHAVNGVAKIHSDIVKTQVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLADLIAEKIGEDYVKDLSQLTKLHSFVSDDIFLREIAKVKQENKLKFSQFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVITMYNRIKKDPKKFFVPRTVIIGGKAAPGYHMAKMIIKLITSVAEVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRVDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPNQPDLFKDIINMLFYHDRFKVFADYEAYVKCQEKVSQLYMNQKAWNTMVLKNIAASGKFSSDRTIKEYAKDIWNMEPSDLKISLSNESSNGVSANGK

Target ORF information:

RefSeq Version	NM_133198.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus liver glycogen phosphorylase (Pygl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133198.2

ORF Insert Sequence:

ATGGCAAAGC CCCTGACCGA CCAGGAGAAG CGACGGCAGA TCAGCATCCG AGGCATCGTG 
GGCGTAGAGA ATGTGGCCGA GCTGAAAAAG GGTTTCAACC GTCACCTGCA CTTCACTCTG 
GTCAAGGACC GCAATGTGGC CACCCCCCGC GACTACTACT TCGCCCTTGC GCACACAGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGT ACACAGCAGC ACTACTACGA CAAGTGTCCC 
AAGAGGGTGT ATTACCTCTC TCTGGAATTT TACATGGGCC GAACATTACA GAACACCATG 
ATCAACCTTG GCTTACAAAA TGCCTGCGAT GAGGCCATTT ACCAGCTTGG ATTGGACATG 
GAAGAGTTAG AAGAAATTGA AGAAGATGCC GGCCTTGGCA ATGGCGGTCT TGGGAGGCTT 
GCTGCCTGCT TCCTGGACTC CATGGCAACC CTGGGACTTG CAGCCTATGG CTACGGCATT 
CGTTATGAAT ACGGAATCTT CAATCAGAAG ATCCGAGAGG GATGGCAGGT AGAAGAGGCA 
GATGACTGGC TCAGGCATGG AAACCCTTGG GAGAAGGCTC GCCCTGAATT CATGCTGCCC 
GTGCATTTCT ACGGAAGAGT AGAGCACACC CAGACGGGGA CAAAGTGGGT CGACACCCAG 
GTGGTCCTGG CTCTGCCTTA CGACACCCCC GTGCCTGGAT ATATGAACAA CACTGTGAAC 
ACTATGCGCC TCTGGTCGGC TCGAGCACCA AATGACTTTA ACCTTCAAGA TTTTAATGTT 
GGAGACTACA TTCAGGCTGT GCTGGACCGG AACCTGGCTG AGAATATCTC CAGAGTGCTC 
TACCCCAATG ATAACTTCTT TGAAGGGAAG GAGTTGCGGC TGAAACAGGA GTACTTTGTG 
GTGGCTGCCA CCCTGCAGGA TGTCATCCGG CGCTTCAAGG CCTCCAAGTT CGGCTCCAAG 
GATGGCATGG GAACCGTGTT TGATGCCTTT CCAGATCAGG TAGCCATCCA GCTGAATGAC 
ACACATCCTG CACTCGCCAT TCCAGAGCTG ATGAGGATTT TTGTGGACAT TGAAAAACTG 
CCCTGGGCCA AGGCATGGGA GATCACGAAG AAGACCTTCG CCTACACCAA CCACACGGTG 
CTCCCGGAGG CCCTGGAGCG CTGGCCGGTG GAACTGGTGG AGAAGCTGCT GCCTCGACAC 
TTGGAGATCA TTTATGAGAT CAATCAGAAA CATTTAGACA GAATTGTGGC CTTGTTTCCT 
AAAGACATCA GCCGCATGCG GAGAATGTCT CTCATTGAGG AGGAAGGAGG CAAACGGATC 
AACATGGCCC ACCTCTGCAT CGTGGGCTGC CACGCGGTGA ACGGTGTAGC AAAGATCCAC 
TCGGACATCG TGAAGACCCA AGTATTCAAG GACTTCAGCG AGCTAGAACC AGACAAGTTC 
CAGAATAAAA CCAACGGGAT TACCCCGAGG CGCTGGCTCC TACTCTGCAA CCCAGGGCTG 
GCTGACTTGA TAGCGGAGAA AATTGGAGAG GATTATGTGA AAGACCTGAG CCAGCTGACG 
AAGCTCCACA GTTTTGTGAG TGATGACATC TTCCTCCGGG AAATAGCCAA AGTGAAACAG 
GAAAATAAGC TGAAATTCTC CCAGTTCCTG GAGAAGGAAT ACAAGGTGAA GATCAACCCA 
TCCTCCATGT TTGATGTCCA TGTGAAGCGG ATCCACGAGT ATAAAAGGCA GCTTCTGAAC 
TGCCTGCATG TGATCACCAT GTACAATCGC ATCAAGAAAG ACCCTAAGAA ATTCTTCGTG 
CCAAGGACAG TCATAATTGG TGGCAAAGCT GCCCCAGGAT ATCACATGGC CAAAATGATC 
ATAAAGCTGA TCACCTCTGT GGCAGAAGTG GTGAACAATG ACCCCATGGT CGGCAGCAAG 
TTGAAAGTCA TCTTCTTGGA GAACTACAGA GTGTCTCTTG CCGAAAAAGT CATTCCAGCC 
ACAGACCTAT CGGAGCAGAT CTCCACGGCA GGCACGGAAG CCTCCGGGAC AGGCAACATG 
AAGTTCATGC TGAACGGGGC CCTGACCATC GGGACTATGG ATGGGGCCAA TGTGGAGATG 
GCAGAGGAAG CTGGGGAGGA GAACCTGTTC ATCTTTGGCA TGAGAGTAGA TGATGTGGCT 
GCTTTGGATA AGAAGGGGTA TGAGGCCAAA GAATATTACG AGGCCCTTCC AGAACTGAAG 
TTGGTCATCG ACCAAATCGA CAATGGCTTC TTTTCTCCCA ATCAGCCAGA CCTCTTCAAA 
GACATCATCA ACATGTTATT TTATCATGAC AGATTTAAAG TCTTTGCAGA CTACGAAGCC 
TATGTCAAGT GTCAAGAAAA AGTCAGTCAG CTGTATATGA ATCAAAAAGC CTGGAACACA 
ATGGTACTCA AAAACATAGC TGCCTCAGGG AAGTTCTCCA GTGACCGAAC AATTAAGGAG 
TATGCCAAGG ACATCTGGAA CATGGAGCCT TCGGATCTGA AGATTTCCCT ATCCAACGAG 
TCCAGCAATG GGGTCAGTGC CAATGGGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepatic Glucocorticoid Receptor Plays a Greater Role Than Adipose GR in Metabolic Syndrome Despite Renal Compensation.
Endocrinology157(12)4943-4960(2016 Dec)
Bose SK,Hutson I,Harris CA

Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is Required for Mitochondrial Translation and Is Involved in Reversible Infantile Liver Injury.
PLoS genetics12(9)e1006355(2016 Sep)
Wu Y,Wei FY,Kawarada L,Suzuki T,Araki K,Komohara Y,Fujimura A,Kaitsuka T,Takeya M,Oike Y,Suzuki T,Tomizawa K

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)
Gurdziel K,Vogt KR,Walton KD,Schneider GK,Gumucio DL

Diabetes risk gene and Wnt effector Tcf7l2/TCF4 controls hepatic response to perinatal and adult metabolic demand.
Cell151(7)1595-607(2012 Dec)
Boj SF,van Es JH,Huch M,Li VS,Jos? A,Hatzis P,Mokry M,Haegebarth A,van den Born M,Chambon P,Voshol P,Dor Y,Cuppen E,Fillat C,Clevers H

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Pygl cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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