Dnaaf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnaaf2
Entrez Gene ID	109065
Full Name	dynein, axonemal assembly factor 2
Synonyms	Ktu,TU54,kintoun,mknt
General protein information	Preferred Names dynein, axonemal assembly factor 2 Names protein kintoun dynein assembly factor 2, axonemal
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C2
Summary	This gene encodes a highly conserved protein involved in the preassembly of dynein arm complexes which power cilia. These complexes are found in some cilia and are assembled in the cytoplasm prior to transport for cilia formation. Mutations in the human gene have been associated with primary ciliary dyskinesia. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_027269.4	NP_081545.3	protein kintoun
NM_027269.3	NP_081545.3	protein kintoun
XM_017314934.2	XP_017170423.1	protein kintoun isoform X1
XM_017314934.1	XP_017170423.1	protein kintoun isoform X1

Pathways from BioSystems

Homologies

	LOC719026	XP_001112551.2
Xenopus tropicalis (tropical clawed frog)	dnaaf2	NP_001072474.1
Bos taurus (cattle)	DNAAF2	NP_001069492.2
Pan troglodytes (chimpanzee)	DNAAF2	XP_509930.3
Mus musculus (house mouse)	Dnaaf2	NP_081545.3
Canis lupus familiaris (dog)	DNAAF2	XP_005623972.1
Rattus norvegicus (Norway rat)	Dnaaf2	NP_001014219.2
Homo sapiens (human)	DNAAF2	NP_060609.2
Gallus gallus (chicken)	DNAAF2	XP_421456.4

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.
Dong F, Shinohara K, Botilde Y, Nabeshima R, Asai Y, Fukumoto A, Hasegawa T, Matsuo M, Takeda H, Shiratori H, Nakamura T, Hamada H
The Journal of cell biology204(2)203-13(2014 Jan)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

The establishment of rotational polarity in the airway and ependymal cilia: analysis with a novel cilium motility mutant mouse.
Matsuo M, Shimada A, Koshida S, Saga Y, Takeda H
American journal of physiology. Lung cellular and molecular physiology304(11)L736-45(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observation of cilia orientation based on basal foot directions revealed that ciliary motility was not required in the alignment of airway cilia, whereas a strong requirement was observed in brain ependymal cells.
Title: The establishment of rotational polarity in the airway and ependymal cilia: analysis with a novel cilium motility mutant mouse.

Ktu/PF13 is one of the long-sought proteins involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport loads them for the ciliary compartment
Title: Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins.

The following Dnaaf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnaaf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03950	NM_027269.4 Latest version!	Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu78129	XM_017314934.2 Latest version!	Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu03950	NM_027269.3	Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu78129	XM_017314934.1	Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnaaf2 ( NM_027269.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081545.3 Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), mRNA.

Price & Availability↑

CloneID	OMu03950
Clone ID Related Accession (Same CDS sequence)	NM_027269.4 , NM_027269.3
Accession Version	NM_027269.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	protein kintoun
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC055807.1 and BC058344.1. On Sep 17, 2015 this sequence version replaced NM_027269.3. ##Evidence-Data-START## Transcript exon combination :: BC055807.1, AB455811.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGG CGGCGGCCTC CTCAGCGCTG GAGGACTTGG ATCTGAGCAG AGAGGAGGTG 
CAGCGGTTCA CCTCCGCCTT TCAGGACCCA GAGTTCCGCC GAATGTTCTC CGAGTACGCC 
GCGGAGATCA CGGACCCCGA GAACCGGAGG CGCTACGAGG AGGAGATCAC AGCCCTGGAG 
CGGGAGCGTG GGGTGGATGT GCGGTTCGTG CACCCTGAGC CGGGCCATGT GCTGCGTACC 
AGCCTGGACG GGGAGCACCG CTGCTACGTG AACGTGTGCA GCAACTCACT GGTAGGCGTA 
CCTAGCAGCC GGCCCGGCCC GGGGCGCGGC GGCACGGCAG CTGGCAGCCA CTGGTCCCTG 
CCCTATAGCC TGGCTCCCGG CCGCCAGTAT GCCGGCCGCA ACGGCGCCCG CTACACCGTC 
TACGACGTGG TCTTCCACCC CGAAGCGCTG GCGCTGGCCC GGAGCCACGA GCGCTTCCGC 
GAAATGCTGG ACGCCACGGC CCTGGAGGCG GTGGAGCAGC AGTTCGGCGT CAGGCTGGAC 
CGCAGGAACG CCAAGACCCT GAAGATCAAG TACAAGGGGA TGCCCGAGGC CGCAGTGCTC 
CGCACGCCCC TACCCGGGGG CGTCCCGGCC CAGCCCGAGG GGGAGCCACC AGGGCTTTTC 
CCGGACCCAC CATATCCATA CCGCTACCCC GCGGCAGCGG CGGCGAACAC TGCCCGCTCG 
CCCGCGTCCC CCGCTCCCGA GGCCGTGCAG CGCCCAGAGC CCACGGAGCC GCGCTGCTCC 
GTGGTGCAGC GCCACCACGT AGACCTCCAG GATTACCGCT GCTCGCGCGA CGCGGCCCCG 
AGCACCGTGC CGCACGAGCT GGTGGTCACC ATCGAGCTGC CGCTGCTGCG CTCGGTCGAG 
CGGGCGGAGC TGGAGGTGAA GGGGAAGCTG CTGTGCCTGG ACTCGAGAAA CCCCGACTAC 
CGGCTGCGGC TCTCGCTGCC GTACCCGGTG GACGACGGCC GCGGCAAGGC ACAGTACAAC 
AAGGCGCGGC GGCAGCTGGT GGTCACGCTG CCGGTGGCGC TGGCCGACGC GCGCCAGGAA 
CCCCCGGCCG CGACACCGGA AGAGCCCGCG GAGGAGACCG GAACTGACGA CGTAGCCCGC 
ACTTCCGCGG GCGATTTTGC AGCGGCGCGG GAAGAGTCTG CAGATGGAAC CGGAGCTGAC 
CACGGGGAGA AGAGCGGTGT GGGAGCCCCA GACCCCGGGG CTGCACACGC TGAAGGGGAG 
CTCGTTCCCG AACCGGAGCA GGACTTCGGA GGGGACTCGG TGGCACCTCT AGACCTCGGG 
AAAGGGACTT CTCCTGGGGA CCGAAGCCTC CCTTACTCTG CTTTCCCGGG CGGCGACACC 
GAATCCCTGT GCGGAGACCC CGGTGTCCAG ACGAATGAGG AGCAGGAGAG GACCCGCCAC 
GACACCGCGG GCTCAGCCAT GGGTGACCCC GGAACCGAGA GCATCGCACC CGTGTGCCCT 
CCTTTACAGT GTAATCAGGA CGAAGACTCC TTGACTTTGC TCATCCAAGT GCCTGGGATT 
CTGCCACAAA CTCTTCACGG ACATCTAAGC CCCGTCGGTT ACGAATTGTG CTTCTCTACA 
CAAGACTCGG GTTATTCCTG CACTTTGCAG TTTGCTCCAG AGAATAAGTT GAGTACCAGA 
GAACCCGAGA CTAGCGTTTC TTTAAATAAT GCAGTCATAG TACTGGCAAA ATCTCCAGAG 
AGCCACGGAC TTTGGCGAGA GTGGTATTGT GGCTTGAACA AGGAGTCTTT GGAGGAAAGA 
TTGTTTATCA ATGAAGAAAA TGTTAATGGG TTTCTTGAAG AGGTCCTTTG CTCCCCATTG 
AAGCAGGCAA GGTCTCTGGC CCCACCGCTG ATTGAGGTTC TTCAGGCCAC TGATGAGCAG 
GTTCAGATCC ATGCAGAGTT GCAGGAATGT AGTGATCCTG CTGGGCTTCA AGGAAAGGGA 
AAAGGAGTCA GAGAAGGCTG TCCTCTGAGT GAAGCAGAAG CCGCTGACCA GTCCGCCACC 
TCTCCAGCTG CTTCTGATTC AGCTGCAGCA GTCGAAGCAC TAAAAATAAA CACGCATGGT 
TCAGCTGTGG ACCTGCAACA CGGCTGTCCT GAGGTTCCTC ACGTACTGTC TGGAAAACCG 
CTGCAACCAG AGGCAAAAAT GGATCCTGAG TTTATAAGAG AAAGTAGTAC TACCTACTCG 
ACTGAGGAAA AGGAAAATAT AAGAGAGCCA GTAATAAGCA AGGGGGAAAA AATAAATGGA 
GATCACCCAT CTTCATTACT GAACAAAACT GTGGTTCAAA ACATACCTGA CTTTGACACT 
ATAAAAGAGA CGAATATGCA GGATGGTAGT GTGCAGATTA TTAGAGACCA CACGACTCAT 
TGTGCATTCA GTTTTCAGAA TCCCTTGCTA TATGATTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081545.3
CDS	145..2589
Translation	MAKAAASSALEDLDLSREEVQRFTSAFQDPEFRRMFSEYAAEITDPENRRRYEEEITALERERGVDVRFVHPEPGHVLRTSLDGEHRCYVNVCSNSLVGVPSSRPGPGRGGTAAGSHWSLPYSLAPGRQYAGRNGARYTVYDVVFHPEALALARSHERFREMLDATALEAVEQQFGVRLDRRNAKTLKIKYKGMPEAAVLRTPLPGGVPAQPEGEPPGLFPDPPYPYRYPAAAAANTARSPASPAPEAVQRPEPTEPRCSVVQRHHVDLQDYRCSRDAAPSTVPHELVVTIELPLLRSVERAELEVKGKLLCLDSRNPDYRLRLSLPYPVDDGRGKAQYNKARRQLVVTLPVALADARQEPPAATPEEPAEETGTDDVARTSAGDFAAAREESADGTGADHGEKSGVGAPDPGAAHAEGELVPEPEQDFGGDSVAPLDLGKGTSPGDRSLPYSAFPGGDTESLCGDPGVQTNEEQERTRHDTAGSAMGDPGTESIAPVCPPLQCNQDEDSLTLLIQVPGILPQTLHGHLSPVGYELCFSTQDSGYSCTLQFAPENKLSTREPETSVSLNNAVIVLAKSPESHGLWREWYCGLNKESLEERLFINEENVNGFLEEVLCSPLKQARSLAPPLIEVLQATDEQVQIHAELQECSDPAGLQGKGKGVREGCPLSEAEAADQSATSPAASDSAAAVEALKINTHGSAVDLQHGCPEVPHVLSGKPLQPEAKMDPEFIRESSTTYSTEEKENIREPVISKGEKINGDHPSSLLNKTVVQNIPDFDTIKETNMQDGSVQIIRDHTTHCAFSFQNPLLYDLD

Target ORF information:

RefSeq Version	NM_027269.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027269.4

ORF Insert Sequence:

ATGGCCAAGG CGGCGGCCTC CTCAGCGCTG GAGGACTTGG ATCTGAGCAG AGAGGAGGTG 
CAGCGGTTCA CCTCCGCCTT TCAGGACCCA GAGTTCCGCC GAATGTTCTC CGAGTACGCC 
GCGGAGATCA CGGACCCCGA GAACCGGAGG CGCTACGAGG AGGAGATCAC AGCCCTGGAG 
CGGGAGCGTG GGGTGGATGT GCGGTTCGTG CACCCTGAGC CGGGCCATGT GCTGCGTACC 
AGCCTGGACG GGGAGCACCG CTGCTACGTG AACGTGTGCA GCAACTCACT GGTAGGCGTA 
CCTAGCAGCC GGCCCGGCCC GGGGCGCGGC GGCACGGCAG CTGGCAGCCA CTGGTCCCTG 
CCCTATAGCC TGGCTCCCGG CCGCCAGTAT GCCGGCCGCA ACGGCGCCCG CTACACCGTC 
TACGACGTGG TCTTCCACCC CGAAGCGCTG GCGCTGGCCC GGAGCCACGA GCGCTTCCGC 
GAAATGCTGG ACGCCACGGC CCTGGAGGCG GTGGAGCAGC AGTTCGGCGT CAGGCTGGAC 
CGCAGGAACG CCAAGACCCT GAAGATCAAG TACAAGGGGA TGCCCGAGGC CGCAGTGCTC 
CGCACGCCCC TACCCGGGGG CGTCCCGGCC CAGCCCGAGG GGGAGCCACC AGGGCTTTTC 
CCGGACCCAC CATATCCATA CCGCTACCCC GCGGCAGCGG CGGCGAACAC TGCCCGCTCG 
CCCGCGTCCC CCGCTCCCGA GGCCGTGCAG CGCCCAGAGC CCACGGAGCC GCGCTGCTCC 
GTGGTGCAGC GCCACCACGT AGACCTCCAG GATTACCGCT GCTCGCGCGA CGCGGCCCCG 
AGCACCGTGC CGCACGAGCT GGTGGTCACC ATCGAGCTGC CGCTGCTGCG CTCGGTCGAG 
CGGGCGGAGC TGGAGGTGAA GGGGAAGCTG CTGTGCCTGG ACTCGAGAAA CCCCGACTAC 
CGGCTGCGGC TCTCGCTGCC GTACCCGGTG GACGACGGCC GCGGCAAGGC ACAGTACAAC 
AAGGCGCGGC GGCAGCTGGT GGTCACGCTG CCGGTGGCGC TGGCCGACGC GCGCCAGGAA 
CCCCCGGCCG CGACACCGGA AGAGCCCGCG GAGGAGACCG GAACTGACGA CGTAGCCCGC 
ACTTCCGCGG GCGATTTTGC AGCGGCGCGG GAAGAGTCTG CAGATGGAAC CGGAGCTGAC 
CACGGGGAGA AGAGCGGTGT GGGAGCCCCA GACCCCGGGG CTGCACACGC TGAAGGGGAG 
CTCGTTCCCG AACCGGAGCA GGACTTCGGA GGGGACTCGG TGGCACCTCT AGACCTCGGG 
AAAGGGACTT CTCCTGGGGA CCGAAGCCTC CCTTACTCTG CTTTCCCGGG CGGCGACACC 
GAATCCCTGT GCGGAGACCC CGGTGTCCAG ACGAATGAGG AGCAGGAGAG GACCCGCCAC 
GACACCGCGG GCTCAGCCAT GGGTGACCCC GGAACCGAGA GCATCGCACC CGTGTGCCCT 
CCTTTACAGT GTAATCAGGA CGAAGACTCC TTGACTTTGC TCATCCAAGT GCCTGGGATT 
CTGCCACAAA CTCTTCACGG ACATCTAAGC CCCGTCGGTT ACGAATTGTG CTTCTCTACA 
CAAGACTCGG GTTATTCCTG CACTTTGCAG TTTGCTCCAG AGAATAAGTT GAGTACCAGA 
GAACCCGAGA CTAGCGTTTC TTTAAATAAT GCAGTCATAG TACTGGCAAA ATCTCCAGAG 
AGCCACGGAC TTTGGCGAGA GTGGTATTGT GGCTTGAACA AGGAGTCTTT GGAGGAAAGA 
TTGTTTATCA ATGAAGAAAA TGTTAATGGG TTTCTTGAAG AGGTCCTTTG CTCCCCATTG 
AAGCAGGCAA GGTCTCTGGC CCCACCGCTG ATTGAGGTTC TTCAGGCCAC TGATGAGCAG 
GTTCAGATCC ATGCAGAGTT GCAGGAATGT AGTGATCCTG CTGGGCTTCA AGGAAAGGGA 
AAAGGAGTCA GAGAAGGCTG TCCTCTGAGT GAAGCAGAAG CCGCTGACCA GTCCGCCACC 
TCTCCAGCTG CTTCTGATTC AGCTGCAGCA GTCGAAGCAC TAAAAATAAA CACGCATGGT 
TCAGCTGTGG ACCTGCAACA CGGCTGTCCT GAGGTTCCTC ACGTACTGTC TGGAAAACCG 
CTGCAACCAG AGGCAAAAAT GGATCCTGAG TTTATAAGAG AAAGTAGTAC TACCTACTCG 
ACTGAGGAAA AGGAAAATAT AAGAGAGCCA GTAATAAGCA AGGGGGAAAA AATAAATGGA 
GATCACCCAT CTTCATTACT GAACAAAACT GTGGTTCAAA ACATACCTGA CTTTGACACT 
ATAAAAGAGA CGAATATGCA GGATGGTAGT GTGCAGATTA TTAGAGACCA CACGACTCAT 
TGTGCATTCA GTTTTCAGAA TCCCTTGCTA TATGATTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnaaf2 ( NM_027269.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081545.3 Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), mRNA.

Price & Availability↑

CloneID	OMu03950
Clone ID Related Accession (Same CDS sequence)	NM_027269.4 , NM_027269.3
Accession Version	NM_027269.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	protein kintoun
Comment

ATGGCCAAGG CGGCGGCCTC CTCAGCGCTG GAGGACTTGG ATCTGAGCAG AGAGGAGGTG 
CAGCGGTTCA CCTCCGCCTT TCAGGACCCA GAGTTCCGCC GAATGTTCTC CGAGTACGCC 
GCGGAGATCA CGGACCCCGA GAACCGGAGG CGCTACGAGG AGGAGATCAC AGCCCTGGAG 
CGGGAGCGTG GGGTGGATGT GCGGTTCGTG CACCCTGAGC CGGGCCATGT GCTGCGTACC 
AGCCTGGACG GGGAGCACCG CTGCTACGTG AACGTGTGCA GCAACTCACT GGTAGGCGTA 
CCTAGCAGCC GGCCCGGCCC GGGGCGCGGC GGCACGGCAG CTGGCAGCCA CTGGTCCCTG 
CCCTATAGCC TGGCTCCCGG CCGCCAGTAT GCCGGCCGCA ACGGCGCCCG CTACACCGTC 
TACGACGTGG TCTTCCACCC CGAAGCGCTG GCGCTGGCCC GGAGCCACGA GCGCTTCCGC 
GAAATGCTGG ACGCCACGGC CCTGGAGGCG GTGGAGCAGC AGTTCGGCGT CAGGCTGGAC 
CGCAGGAACG CCAAGACCCT GAAGATCAAG TACAAGGGGA TGCCCGAGGC CGCAGTGCTC 
CGCACGCCCC TACCCGGGGG CGTCCCGGCC CAGCCCGAGG GGGAGCCACC AGGGCTTTTC 
CCGGACCCAC CATATCCATA CCGCTACCCC GCGGCAGCGG CGGCGAACAC TGCCCGCTCG 
CCCGCGTCCC CCGCTCCCGA GGCCGTGCAG CGCCCAGAGC CCACGGAGCC GCGCTGCTCC 
GTGGTGCAGC GCCACCACGT AGACCTCCAG GATTACCGCT GCTCGCGCGA CGCGGCCCCG 
AGCACCGTGC CGCACGAGCT GGTGGTCACC ATCGAGCTGC CGCTGCTGCG CTCGGTCGAG 
CGGGCGGAGC TGGAGGTGAA GGGGAAGCTG CTGTGCCTGG ACTCGAGAAA CCCCGACTAC 
CGGCTGCGGC TCTCGCTGCC GTACCCGGTG GACGACGGCC GCGGCAAGGC ACAGTACAAC 
AAGGCGCGGC GGCAGCTGGT GGTCACGCTG CCGGTGGCGC TGGCCGACGC GCGCCAGGAA 
CCCCCGGCCG CGACACCGGA AGAGCCCGCG GAGGAGACCG GAACTGACGA CGTAGCCCGC 
ACTTCCGCGG GCGATTTTGC AGCGGCGCGG GAAGAGTCTG CAGATGGAAC CGGAGCTGAC 
CACGGGGAGA AGAGCGGTGT GGGAGCCCCA GACCCCGGGG CTGCACACGC TGAAGGGGAG 
CTCGTTCCCG AACCGGAGCA GGACTTCGGA GGGGACTCGG TGGCACCTCT AGACCTCGGG 
AAAGGGACTT CTCCTGGGGA CCGAAGCCTC CCTTACTCTG CTTTCCCGGG CGGCGACACC 
GAATCCCTGT GCGGAGACCC CGGTGTCCAG ACGAATGAGG AGCAGGAGAG GACCCGCCAC 
GACACCGCGG GCTCAGCCAT GGGTGACCCC GGAACCGAGA GCATCGCACC CGTGTGCCCT 
CCTTTACAGT GTAATCAGGA CGAAGACTCC TTGACTTTGC TCATCCAAGT GCCTGGGATT 
CTGCCACAAA CTCTTCACGG ACATCTAAGC CCCGTCGGTT ACGAATTGTG CTTCTCTACA 
CAAGACTCGG GTTATTCCTG CACTTTGCAG TTTGCTCCAG AGAATAAGTT GAGTACCAGA 
GAACCCGAGA CTAGCGTTTC TTTAAATAAT GCAGTCATAG TACTGGCAAA ATCTCCAGAG 
AGCCACGGAC TTTGGCGAGA GTGGTATTGT GGCTTGAACA AGGAGTCTTT GGAGGAAAGA 
TTGTTTATCA ATGAAGAAAA TGTTAATGGG TTTCTTGAAG AGGTCCTTTG CTCCCCATTG 
AAGCAGGCAA GGTCTCTGGC CCCACCGCTG ATTGAGGTTC TTCAGGCCAC TGATGAGCAG 
GTTCAGATCC ATGCAGAGTT GCAGGAATGT AGTGATCCTG CTGGGCTTCA AGGAAAGGGA 
AAAGGAGTCA GAGAAGGCTG TCCTCTGAGT GAAGCAGAAG CCGCTGACCA GTCCGCCACC 
TCTCCAGCTG CTTCTGATTC AGCTGCAGCA GTCGAAGCAC TAAAAATAAA CACGCATGGT 
TCAGCTGTGG ACCTGCAACA CGGCTGTCCT GAGGTTCCTC ACGTACTGTC TGGAAAACCG 
CTGCAACCAG AGGCAAAAAT GGATCCTGAG TTTATAAGAG AAAGTAGTAC TACCTACTCG 
ACTGAGGAAA AGGAAAATAT AAGAGAGCCA GTAATAAGCA AGGGGGAAAA AATAAATGGA 
GATCACCCAT CTTCATTACT GAACAAAACT GTGGTTCAAA ACATACCTGA CTTTGACACT 
ATAAAAGAGA CGAATATGCA GGATGGTAGT GTGCAGATTA TTAGAGACCA CACGACTCAT 
TGTGCATTCA GTTTTCAGAA TCCCTTGCTA TATGATTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_081545.3

Translation

MAKAAASSALEDLDLSREEVQRFTSAFQDPEFRRMFSEYAAEITDPENRRRYEEEITALERERGVDVRFVHPEPGHVLRTSLDGEHRCYVNVCSNSLVGVPSSRPGPGRGGTAAGSHWSLPYSLAPGRQYAGRNGARYTVYDVVFHPEALALARSHERFREMLDATALEAVEQQFGVRLDRRNAKTLKIKYKGMPEAAVLRTPLPGGVPAQPEGEPPGLFPDPPYPYRYPAAAAANTARSPASPAPEAVQRPEPTEPRCSVVQRHHVDLQDYRCSRDAAPSTVPHELVVTIELPLLRSVERAELEVKGKLLCLDSRNPDYRLRLSLPYPVDDGRGKAQYNKARRQLVVTLPVALADARQEPPAATPEEPAEETGTDDVARTSAGDFAAAREESADGTGADHGEKSGVGAPDPGAAHAEGELVPEPEQDFGGDSVAPLDLGKGTSPGDRSLPYSAFPGGDTESLCGDPGVQTNEEQERTRHDTAGSAMGDPGTESIAPVCPPLQCNQDEDSLTLLIQVPGILPQTLHGHLSPVGYELCFSTQDSGYSCTLQFAPENKLSTREPETSVSLNNAVIVLAKSPESHGLWREWYCGLNKESLEERLFINEENVNGFLEEVLCSPLKQARSLAPPLIEVLQATDEQVQIHAELQECSDPAGLQGKGKGVREGCPLSEAEAADQSATSPAASDSAAAVEALKINTHGSAVDLQHGCPEVPHVLSGKPLQPEAKMDPEFIRESSTTYSTEEKENIREPVISKGEKINGDHPSSLLNKTVVQNIPDFDTIKETNMQDGSVQIIRDHTTHCAFSFQNPLLYDLD

Target ORF information:

RefSeq Version	NM_027269.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027269.3

ORF Insert Sequence:

ATGGCCAAGG CGGCGGCCTC CTCAGCGCTG GAGGACTTGG ATCTGAGCAG AGAGGAGGTG 
CAGCGGTTCA CCTCCGCCTT TCAGGACCCA GAGTTCCGCC GAATGTTCTC CGAGTACGCC 
GCGGAGATCA CGGACCCCGA GAACCGGAGG CGCTACGAGG AGGAGATCAC AGCCCTGGAG 
CGGGAGCGTG GGGTGGATGT GCGGTTCGTG CACCCTGAGC CGGGCCATGT GCTGCGTACC 
AGCCTGGACG GGGAGCACCG CTGCTACGTG AACGTGTGCA GCAACTCACT GGTAGGCGTA 
CCTAGCAGCC GGCCCGGCCC GGGGCGCGGC GGCACGGCAG CTGGCAGCCA CTGGTCCCTG 
CCCTATAGCC TGGCTCCCGG CCGCCAGTAT GCCGGCCGCA ACGGCGCCCG CTACACCGTC 
TACGACGTGG TCTTCCACCC CGAAGCGCTG GCGCTGGCCC GGAGCCACGA GCGCTTCCGC 
GAAATGCTGG ACGCCACGGC CCTGGAGGCG GTGGAGCAGC AGTTCGGCGT CAGGCTGGAC 
CGCAGGAACG CCAAGACCCT GAAGATCAAG TACAAGGGGA TGCCCGAGGC CGCAGTGCTC 
CGCACGCCCC TACCCGGGGG CGTCCCGGCC CAGCCCGAGG GGGAGCCACC AGGGCTTTTC 
CCGGACCCAC CATATCCATA CCGCTACCCC GCGGCAGCGG CGGCGAACAC TGCCCGCTCG 
CCCGCGTCCC CCGCTCCCGA GGCCGTGCAG CGCCCAGAGC CCACGGAGCC GCGCTGCTCC 
GTGGTGCAGC GCCACCACGT AGACCTCCAG GATTACCGCT GCTCGCGCGA CGCGGCCCCG 
AGCACCGTGC CGCACGAGCT GGTGGTCACC ATCGAGCTGC CGCTGCTGCG CTCGGTCGAG 
CGGGCGGAGC TGGAGGTGAA GGGGAAGCTG CTGTGCCTGG ACTCGAGAAA CCCCGACTAC 
CGGCTGCGGC TCTCGCTGCC GTACCCGGTG GACGACGGCC GCGGCAAGGC ACAGTACAAC 
AAGGCGCGGC GGCAGCTGGT GGTCACGCTG CCGGTGGCGC TGGCCGACGC GCGCCAGGAA 
CCCCCGGCCG CGACACCGGA AGAGCCCGCG GAGGAGACCG GAACTGACGA CGTAGCCCGC 
ACTTCCGCGG GCGATTTTGC AGCGGCGCGG GAAGAGTCTG CAGATGGAAC CGGAGCTGAC 
CACGGGGAGA AGAGCGGTGT GGGAGCCCCA GACCCCGGGG CTGCACACGC TGAAGGGGAG 
CTCGTTCCCG AACCGGAGCA GGACTTCGGA GGGGACTCGG TGGCACCTCT AGACCTCGGG 
AAAGGGACTT CTCCTGGGGA CCGAAGCCTC CCTTACTCTG CTTTCCCGGG CGGCGACACC 
GAATCCCTGT GCGGAGACCC CGGTGTCCAG ACGAATGAGG AGCAGGAGAG GACCCGCCAC 
GACACCGCGG GCTCAGCCAT GGGTGACCCC GGAACCGAGA GCATCGCACC CGTGTGCCCT 
CCTTTACAGT GTAATCAGGA CGAAGACTCC TTGACTTTGC TCATCCAAGT GCCTGGGATT 
CTGCCACAAA CTCTTCACGG ACATCTAAGC CCCGTCGGTT ACGAATTGTG CTTCTCTACA 
CAAGACTCGG GTTATTCCTG CACTTTGCAG TTTGCTCCAG AGAATAAGTT GAGTACCAGA 
GAACCCGAGA CTAGCGTTTC TTTAAATAAT GCAGTCATAG TACTGGCAAA ATCTCCAGAG 
AGCCACGGAC TTTGGCGAGA GTGGTATTGT GGCTTGAACA AGGAGTCTTT GGAGGAAAGA 
TTGTTTATCA ATGAAGAAAA TGTTAATGGG TTTCTTGAAG AGGTCCTTTG CTCCCCATTG 
AAGCAGGCAA GGTCTCTGGC CCCACCGCTG ATTGAGGTTC TTCAGGCCAC TGATGAGCAG 
GTTCAGATCC ATGCAGAGTT GCAGGAATGT AGTGATCCTG CTGGGCTTCA AGGAAAGGGA 
AAAGGAGTCA GAGAAGGCTG TCCTCTGAGT GAAGCAGAAG CCGCTGACCA GTCCGCCACC 
TCTCCAGCTG CTTCTGATTC AGCTGCAGCA GTCGAAGCAC TAAAAATAAA CACGCATGGT 
TCAGCTGTGG ACCTGCAACA CGGCTGTCCT GAGGTTCCTC ACGTACTGTC TGGAAAACCG 
CTGCAACCAG AGGCAAAAAT GGATCCTGAG TTTATAAGAG AAAGTAGTAC TACCTACTCG 
ACTGAGGAAA AGGAAAATAT AAGAGAGCCA GTAATAAGCA AGGGGGAAAA AATAAATGGA 
GATCACCCAT CTTCATTACT GAACAAAACT GTGGTTCAAA ACATACCTGA CTTTGACACT 
ATAAAAGAGA CGAATATGCA GGATGGTAGT GTGCAGATTA TTAGAGACCA CACGACTCAT 
TGTGCATTCA GTTTTCAGAA TCCCTTGCTA TATGATTTAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnaaf2 ( XM_017314934.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170423.1 Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78129
Clone ID Related Accession (Same CDS sequence)	XM_017314934.2 , XM_017314934.1
Accession Version	XM_017314934.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein kintoun isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG CGGCGGCCTC CTCAGCGCTG GAGGACTTGG ATCTGAGCAG AGAGGAGGTG 
CAGCGGTTCA CCTCCGCCTT TCAGGACCCA GAGTTCCGCC GAATGTTCTC CGAGTACGCC 
GCGGAGATCA CGGACCCCGA GAACCGGAGG CGCTACGAGG AGGAGATCAC AGCCCTGGAG 
CGGGAGCGTG GGGTGGATGT GCGGTTCGTG CACCCTGAGC CGGGCCATGT GCTGCGTACC 
AGCCTGGACG GGGAGCACCG CTGCTACGTG AACGTGTGCA GCAACTCACT GGTAGGCGTA 
CCTAGCAGCC GGCCCGGCCC GGGGCGCGGC GGCACGGCAG CTGGCAGCCA CTGGTCCCTG 
CCCTATAGCC TGGCTCCCGG CCGCCAGTAT GCCGGCCGCA ACGGCGCCCG CTACACCGTC 
TACGACGTGG TCTTCCACCC CGAAGCGCTG GCGCTGGCCC GGAGCCACGA GCGCTTCCGC 
GAAATGCTGG ACGCCACGGC CCTGGAGGCG GTGGAGCAGC AGTTCGGCGT CAGGCTGGAC 
CGCAGGAACG CCAAGACCCT GAAGATCAAG TACAAGGGGA TGCCCGAGGC CGCAGTGCTC 
CGCACGCCCC TACCCGGGGG CGTCCCGGCC CAGCCCGAGG GGGAGCCACC AGGGCTTTTC 
CCGGACCCAC CATATCCATA CCGCTACCCC GCGGCAGCGG CGGCGAACAC TGCCCGCTCG 
CCCGCGTCCC CCGCTCCCGA GGCCGTGCAG CGCCCAGAGC CCACGGAGCC GCGCTGCTCC 
GTGGTGCAGC GCCACCACGT AGACCTCCAG GATTACCGCT GCTCGCGCGA CGCGGCCCCG 
AGCACCGTGC CGCACGAGCT GGTGGTCACC ATCGAGCTGC CGCTGCTGCG CTCGGTCGAG 
CGGGCGGAGC TGGAGGTGAA GGGGAAGCTG CTGTGCCTGG ACTCGAGAAA CCCCGACTAC 
CGGCTGCGGC TCTCGCTGCC GTACCCGGTG GACGACGGCC GCGGCAAGGC ACAGTACAAC 
AAGGCGCGGC GGCAGCTGGT GGTCACGCTG CCGGTGGCGC TGGCCGACGC GCGCCAGGAA 
CCCCCGGCCG CGACACCGGA AGAGCCCGCG GAGGAGACCG GAACTGACGA CGTAGCCCGC 
ACTTCCGCGG GCGATTTTGC AGCGGCGCGG GAAGAGTCTG CAGATGGAAC CGGAGCTGAC 
CACGGGGAGA AGAGCGGTGT GGGAGCCCCA GACCCCGGGG CTGCACACGC TGAAGGGGAG 
CTCGTTCCCG AACCGGAGCA GGACTTCGGA GGGGACTCGG TGGCACCTCT AGACCTCGGG 
AAAGGGACTT CTCCTGGGGA CCGAAGCCTC CCTTACTCTG CTTTCCCGGG CGGCGACACC 
GAATCCCTGT GCGGAGACCC CGGTGTCCAG ACGAATGAGG AGCAGGAGAG GACCCGCCAC 
GACACCGCGG GCTCAGCCAT GGGTGACCCC GGAACCGAGA GCATCGCACC CGTGTGCCCT 
CCTTTACAGT GTAATCAGGA CGAAGACTCC TTGACTTTGC TCATCCAAGT GCCTGGGATT 
CTGCCACAAA CTCTTCACGG ACATCTAAGC CCCGTCGGTT ACGAATTGTG CTTCTCTACA 
CAAGACTCGG GTTATTCCTG CACTTTGCAG TTTGCTCCAG AGAATAAGTT GAGTACCAGA 
GAACCCGAGA CTAGCGTTTC TTTAAATAAT GCAGTCATAG TACTGGCAAA ATCTCCAGAG 
AGCCACGGAC TTTGGCGAGA GTGGTATTGT GGCTTGAACA AGGAGTCTTT GGAGTTGCAG 
GAATGTAGTG ATCCTGCTGG GCTTCAAGGA AAGGGAAAAG GAGTCAGAGA AGGCTGTCCT 
CTGAGTGAAG CAGAAGCCGC TGACCAGTCC GCCACCTCTC CAGCTGCTTC TGATTCAGCT 
GCAGCAGTCG AAGCACTAAA AATAAACACG CATGGTTCAG CTGTGGACCT GCAACACGGC 
TGTCCTGAGG TTCCTCACGT ACTGTCTGGA AAACCGCTGC AACCAGAGGC AAAAATGGAT 
CCTGAGTTTA TAAGAGAAAG TAGTACTACC TACTCGACTG AGGAAAAGGA AAATATAAGA 
GAGCCAGTAA TAAGCAAGGG GGAAAAAATA AATGGAGATC ACCCATCTTC ATTACTGAAC 
AAAACTGTGG TTCAAAACAT ACCTGACTTT GACACTATAA AAGAGACGAA TATGCAGGAT 
GGTAGTGTGC AGATTATTAG AGACCACACG ACTCATTGTG CATTCAGTTT TCAGAATCCC 
TTGCTATATG ATTTAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170423.1
CDS	1799..4099
Translation	MAKAAASSALEDLDLSREEVQRFTSAFQDPEFRRMFSEYAAEITDPENRRRYEEEITALERERGVDVRFVHPEPGHVLRTSLDGEHRCYVNVCSNSLVGVPSSRPGPGRGGTAAGSHWSLPYSLAPGRQYAGRNGARYTVYDVVFHPEALALARSHERFREMLDATALEAVEQQFGVRLDRRNAKTLKIKYKGMPEAAVLRTPLPGGVPAQPEGEPPGLFPDPPYPYRYPAAAAANTARSPASPAPEAVQRPEPTEPRCSVVQRHHVDLQDYRCSRDAAPSTVPHELVVTIELPLLRSVERAELEVKGKLLCLDSRNPDYRLRLSLPYPVDDGRGKAQYNKARRQLVVTLPVALADARQEPPAATPEEPAEETGTDDVARTSAGDFAAAREESADGTGADHGEKSGVGAPDPGAAHAEGELVPEPEQDFGGDSVAPLDLGKGTSPGDRSLPYSAFPGGDTESLCGDPGVQTNEEQERTRHDTAGSAMGDPGTESIAPVCPPLQCNQDEDSLTLLIQVPGILPQTLHGHLSPVGYELCFSTQDSGYSCTLQFAPENKLSTREPETSVSLNNAVIVLAKSPESHGLWREWYCGLNKESLELQECSDPAGLQGKGKGVREGCPLSEAEAADQSATSPAASDSAAAVEALKINTHGSAVDLQHGCPEVPHVLSGKPLQPEAKMDPEFIRESSTTYSTEEKENIREPVISKGEKINGDHPSSLLNKTVVQNIPDFDTIKETNMQDGSVQIIRDHTTHCAFSFQNPLLYDLD

Target ORF information:

RefSeq Version	XM_017314934.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314934.2

ORF Insert Sequence:

ATGGCCAAGG CGGCGGCCTC CTCAGCGCTG GAGGACTTGG ATCTGAGCAG AGAGGAGGTG 
CAGCGGTTCA CCTCCGCCTT TCAGGACCCA GAGTTCCGCC GAATGTTCTC CGAGTACGCC 
GCGGAGATCA CGGACCCCGA GAACCGGAGG CGCTACGAGG AGGAGATCAC AGCCCTGGAG 
CGGGAGCGTG GGGTGGATGT GCGGTTCGTG CACCCTGAGC CGGGCCATGT GCTGCGTACC 
AGCCTGGACG GGGAGCACCG CTGCTACGTG AACGTGTGCA GCAACTCACT GGTAGGCGTA 
CCTAGCAGCC GGCCCGGCCC GGGGCGCGGC GGCACGGCAG CTGGCAGCCA CTGGTCCCTG 
CCCTATAGCC TGGCTCCCGG CCGCCAGTAT GCCGGCCGCA ACGGCGCCCG CTACACCGTC 
TACGACGTGG TCTTCCACCC CGAAGCGCTG GCGCTGGCCC GGAGCCACGA GCGCTTCCGC 
GAAATGCTGG ACGCCACGGC CCTGGAGGCG GTGGAGCAGC AGTTCGGCGT CAGGCTGGAC 
CGCAGGAACG CCAAGACCCT GAAGATCAAG TACAAGGGGA TGCCCGAGGC CGCAGTGCTC 
CGCACGCCCC TACCCGGGGG CGTCCCGGCC CAGCCCGAGG GGGAGCCACC AGGGCTTTTC 
CCGGACCCAC CATATCCATA CCGCTACCCC GCGGCAGCGG CGGCGAACAC TGCCCGCTCG 
CCCGCGTCCC CCGCTCCCGA GGCCGTGCAG CGCCCAGAGC CCACGGAGCC GCGCTGCTCC 
GTGGTGCAGC GCCACCACGT AGACCTCCAG GATTACCGCT GCTCGCGCGA CGCGGCCCCG 
AGCACCGTGC CGCACGAGCT GGTGGTCACC ATCGAGCTGC CGCTGCTGCG CTCGGTCGAG 
CGGGCGGAGC TGGAGGTGAA GGGGAAGCTG CTGTGCCTGG ACTCGAGAAA CCCCGACTAC 
CGGCTGCGGC TCTCGCTGCC GTACCCGGTG GACGACGGCC GCGGCAAGGC ACAGTACAAC 
AAGGCGCGGC GGCAGCTGGT GGTCACGCTG CCGGTGGCGC TGGCCGACGC GCGCCAGGAA 
CCCCCGGCCG CGACACCGGA AGAGCCCGCG GAGGAGACCG GAACTGACGA CGTAGCCCGC 
ACTTCCGCGG GCGATTTTGC AGCGGCGCGG GAAGAGTCTG CAGATGGAAC CGGAGCTGAC 
CACGGGGAGA AGAGCGGTGT GGGAGCCCCA GACCCCGGGG CTGCACACGC TGAAGGGGAG 
CTCGTTCCCG AACCGGAGCA GGACTTCGGA GGGGACTCGG TGGCACCTCT AGACCTCGGG 
AAAGGGACTT CTCCTGGGGA CCGAAGCCTC CCTTACTCTG CTTTCCCGGG CGGCGACACC 
GAATCCCTGT GCGGAGACCC CGGTGTCCAG ACGAATGAGG AGCAGGAGAG GACCCGCCAC 
GACACCGCGG GCTCAGCCAT GGGTGACCCC GGAACCGAGA GCATCGCACC CGTGTGCCCT 
CCTTTACAGT GTAATCAGGA CGAAGACTCC TTGACTTTGC TCATCCAAGT GCCTGGGATT 
CTGCCACAAA CTCTTCACGG ACATCTAAGC CCCGTCGGTT ACGAATTGTG CTTCTCTACA 
CAAGACTCGG GTTATTCCTG CACTTTGCAG TTTGCTCCAG AGAATAAGTT GAGTACCAGA 
GAACCCGAGA CTAGCGTTTC TTTAAATAAT GCAGTCATAG TACTGGCAAA ATCTCCAGAG 
AGCCACGGAC TTTGGCGAGA GTGGTATTGT GGCTTGAACA AGGAGTCTTT GGAGTTGCAG 
GAATGTAGTG ATCCTGCTGG GCTTCAAGGA AAGGGAAAAG GAGTCAGAGA AGGCTGTCCT 
CTGAGTGAAG CAGAAGCCGC TGACCAGTCC GCCACCTCTC CAGCTGCTTC TGATTCAGCT 
GCAGCAGTCG AAGCACTAAA AATAAACACG CATGGTTCAG CTGTGGACCT GCAACACGGC 
TGTCCTGAGG TTCCTCACGT ACTGTCTGGA AAACCGCTGC AACCAGAGGC AAAAATGGAT 
CCTGAGTTTA TAAGAGAAAG TAGTACTACC TACTCGACTG AGGAAAAGGA AAATATAAGA 
GAGCCAGTAA TAAGCAAGGG GGAAAAAATA AATGGAGATC ACCCATCTTC ATTACTGAAC 
AAAACTGTGG TTCAAAACAT ACCTGACTTT GACACTATAA AAGAGACGAA TATGCAGGAT 
GGTAGTGTGC AGATTATTAG AGACCACACG ACTCATTGTG CATTCAGTTT TCAGAATCCC 
TTGCTATATG ATTTAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnaaf2 ( XM_017314934.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170423.1 Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78129
Clone ID Related Accession (Same CDS sequence)	XM_017314934.2 , XM_017314934.1
Accession Version	XM_017314934.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein kintoun isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG CGGCGGCCTC CTCAGCGCTG GAGGACTTGG ATCTGAGCAG AGAGGAGGTG 
CAGCGGTTCA CCTCCGCCTT TCAGGACCCA GAGTTCCGCC GAATGTTCTC CGAGTACGCC 
GCGGAGATCA CGGACCCCGA GAACCGGAGG CGCTACGAGG AGGAGATCAC AGCCCTGGAG 
CGGGAGCGTG GGGTGGATGT GCGGTTCGTG CACCCTGAGC CGGGCCATGT GCTGCGTACC 
AGCCTGGACG GGGAGCACCG CTGCTACGTG AACGTGTGCA GCAACTCACT GGTAGGCGTA 
CCTAGCAGCC GGCCCGGCCC GGGGCGCGGC GGCACGGCAG CTGGCAGCCA CTGGTCCCTG 
CCCTATAGCC TGGCTCCCGG CCGCCAGTAT GCCGGCCGCA ACGGCGCCCG CTACACCGTC 
TACGACGTGG TCTTCCACCC CGAAGCGCTG GCGCTGGCCC GGAGCCACGA GCGCTTCCGC 
GAAATGCTGG ACGCCACGGC CCTGGAGGCG GTGGAGCAGC AGTTCGGCGT CAGGCTGGAC 
CGCAGGAACG CCAAGACCCT GAAGATCAAG TACAAGGGGA TGCCCGAGGC CGCAGTGCTC 
CGCACGCCCC TACCCGGGGG CGTCCCGGCC CAGCCCGAGG GGGAGCCACC AGGGCTTTTC 
CCGGACCCAC CATATCCATA CCGCTACCCC GCGGCAGCGG CGGCGAACAC TGCCCGCTCG 
CCCGCGTCCC CCGCTCCCGA GGCCGTGCAG CGCCCAGAGC CCACGGAGCC GCGCTGCTCC 
GTGGTGCAGC GCCACCACGT AGACCTCCAG GATTACCGCT GCTCGCGCGA CGCGGCCCCG 
AGCACCGTGC CGCACGAGCT GGTGGTCACC ATCGAGCTGC CGCTGCTGCG CTCGGTCGAG 
CGGGCGGAGC TGGAGGTGAA GGGGAAGCTG CTGTGCCTGG ACTCGAGAAA CCCCGACTAC 
CGGCTGCGGC TCTCGCTGCC GTACCCGGTG GACGACGGCC GCGGCAAGGC ACAGTACAAC 
AAGGCGCGGC GGCAGCTGGT GGTCACGCTG CCGGTGGCGC TGGCCGACGC GCGCCAGGAA 
CCCCCGGCCG CGACACCGGA AGAGCCCGCG GAGGAGACCG GAACTGACGA CGTAGCCCGC 
ACTTCCGCGG GCGATTTTGC AGCGGCGCGG GAAGAGTCTG CAGATGGAAC CGGAGCTGAC 
CACGGGGAGA AGAGCGGTGT GGGAGCCCCA GACCCCGGGG CTGCACACGC TGAAGGGGAG 
CTCGTTCCCG AACCGGAGCA GGACTTCGGA GGGGACTCGG TGGCACCTCT AGACCTCGGG 
AAAGGGACTT CTCCTGGGGA CCGAAGCCTC CCTTACTCTG CTTTCCCGGG CGGCGACACC 
GAATCCCTGT GCGGAGACCC CGGTGTCCAG ACGAATGAGG AGCAGGAGAG GACCCGCCAC 
GACACCGCGG GCTCAGCCAT GGGTGACCCC GGAACCGAGA GCATCGCACC CGTGTGCCCT 
CCTTTACAGT GTAATCAGGA CGAAGACTCC TTGACTTTGC TCATCCAAGT GCCTGGGATT 
CTGCCACAAA CTCTTCACGG ACATCTAAGC CCCGTCGGTT ACGAATTGTG CTTCTCTACA 
CAAGACTCGG GTTATTCCTG CACTTTGCAG TTTGCTCCAG AGAATAAGTT GAGTACCAGA 
GAACCCGAGA CTAGCGTTTC TTTAAATAAT GCAGTCATAG TACTGGCAAA ATCTCCAGAG 
AGCCACGGAC TTTGGCGAGA GTGGTATTGT GGCTTGAACA AGGAGTCTTT GGAGTTGCAG 
GAATGTAGTG ATCCTGCTGG GCTTCAAGGA AAGGGAAAAG GAGTCAGAGA AGGCTGTCCT 
CTGAGTGAAG CAGAAGCCGC TGACCAGTCC GCCACCTCTC CAGCTGCTTC TGATTCAGCT 
GCAGCAGTCG AAGCACTAAA AATAAACACG CATGGTTCAG CTGTGGACCT GCAACACGGC 
TGTCCTGAGG TTCCTCACGT ACTGTCTGGA AAACCGCTGC AACCAGAGGC AAAAATGGAT 
CCTGAGTTTA TAAGAGAAAG TAGTACTACC TACTCGACTG AGGAAAAGGA AAATATAAGA 
GAGCCAGTAA TAAGCAAGGG GGAAAAAATA AATGGAGATC ACCCATCTTC ATTACTGAAC 
AAAACTGTGG TTCAAAACAT ACCTGACTTT GACACTATAA AAGAGACGAA TATGCAGGAT 
GGTAGTGTGC AGATTATTAG AGACCACACG ACTCATTGTG CATTCAGTTT TCAGAATCCC 
TTGCTATATG ATTTAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170423.1
CDS	88..2388
Translation	MAKAAASSALEDLDLSREEVQRFTSAFQDPEFRRMFSEYAAEITDPENRRRYEEEITALERERGVDVRFVHPEPGHVLRTSLDGEHRCYVNVCSNSLVGVPSSRPGPGRGGTAAGSHWSLPYSLAPGRQYAGRNGARYTVYDVVFHPEALALARSHERFREMLDATALEAVEQQFGVRLDRRNAKTLKIKYKGMPEAAVLRTPLPGGVPAQPEGEPPGLFPDPPYPYRYPAAAAANTARSPASPAPEAVQRPEPTEPRCSVVQRHHVDLQDYRCSRDAAPSTVPHELVVTIELPLLRSVERAELEVKGKLLCLDSRNPDYRLRLSLPYPVDDGRGKAQYNKARRQLVVTLPVALADARQEPPAATPEEPAEETGTDDVARTSAGDFAAAREESADGTGADHGEKSGVGAPDPGAAHAEGELVPEPEQDFGGDSVAPLDLGKGTSPGDRSLPYSAFPGGDTESLCGDPGVQTNEEQERTRHDTAGSAMGDPGTESIAPVCPPLQCNQDEDSLTLLIQVPGILPQTLHGHLSPVGYELCFSTQDSGYSCTLQFAPENKLSTREPETSVSLNNAVIVLAKSPESHGLWREWYCGLNKESLELQECSDPAGLQGKGKGVREGCPLSEAEAADQSATSPAASDSAAAVEALKINTHGSAVDLQHGCPEVPHVLSGKPLQPEAKMDPEFIRESSTTYSTEEKENIREPVISKGEKINGDHPSSLLNKTVVQNIPDFDTIKETNMQDGSVQIIRDHTTHCAFSFQNPLLYDLD

Target ORF information:

RefSeq Version	XM_017314934.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dynein, axonemal assembly factor 2 (Dnaaf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314934.1

ORF Insert Sequence:

ATGGCCAAGG CGGCGGCCTC CTCAGCGCTG GAGGACTTGG ATCTGAGCAG AGAGGAGGTG 
CAGCGGTTCA CCTCCGCCTT TCAGGACCCA GAGTTCCGCC GAATGTTCTC CGAGTACGCC 
GCGGAGATCA CGGACCCCGA GAACCGGAGG CGCTACGAGG AGGAGATCAC AGCCCTGGAG 
CGGGAGCGTG GGGTGGATGT GCGGTTCGTG CACCCTGAGC CGGGCCATGT GCTGCGTACC 
AGCCTGGACG GGGAGCACCG CTGCTACGTG AACGTGTGCA GCAACTCACT GGTAGGCGTA 
CCTAGCAGCC GGCCCGGCCC GGGGCGCGGC GGCACGGCAG CTGGCAGCCA CTGGTCCCTG 
CCCTATAGCC TGGCTCCCGG CCGCCAGTAT GCCGGCCGCA ACGGCGCCCG CTACACCGTC 
TACGACGTGG TCTTCCACCC CGAAGCGCTG GCGCTGGCCC GGAGCCACGA GCGCTTCCGC 
GAAATGCTGG ACGCCACGGC CCTGGAGGCG GTGGAGCAGC AGTTCGGCGT CAGGCTGGAC 
CGCAGGAACG CCAAGACCCT GAAGATCAAG TACAAGGGGA TGCCCGAGGC CGCAGTGCTC 
CGCACGCCCC TACCCGGGGG CGTCCCGGCC CAGCCCGAGG GGGAGCCACC AGGGCTTTTC 
CCGGACCCAC CATATCCATA CCGCTACCCC GCGGCAGCGG CGGCGAACAC TGCCCGCTCG 
CCCGCGTCCC CCGCTCCCGA GGCCGTGCAG CGCCCAGAGC CCACGGAGCC GCGCTGCTCC 
GTGGTGCAGC GCCACCACGT AGACCTCCAG GATTACCGCT GCTCGCGCGA CGCGGCCCCG 
AGCACCGTGC CGCACGAGCT GGTGGTCACC ATCGAGCTGC CGCTGCTGCG CTCGGTCGAG 
CGGGCGGAGC TGGAGGTGAA GGGGAAGCTG CTGTGCCTGG ACTCGAGAAA CCCCGACTAC 
CGGCTGCGGC TCTCGCTGCC GTACCCGGTG GACGACGGCC GCGGCAAGGC ACAGTACAAC 
AAGGCGCGGC GGCAGCTGGT GGTCACGCTG CCGGTGGCGC TGGCCGACGC GCGCCAGGAA 
CCCCCGGCCG CGACACCGGA AGAGCCCGCG GAGGAGACCG GAACTGACGA CGTAGCCCGC 
ACTTCCGCGG GCGATTTTGC AGCGGCGCGG GAAGAGTCTG CAGATGGAAC CGGAGCTGAC 
CACGGGGAGA AGAGCGGTGT GGGAGCCCCA GACCCCGGGG CTGCACACGC TGAAGGGGAG 
CTCGTTCCCG AACCGGAGCA GGACTTCGGA GGGGACTCGG TGGCACCTCT AGACCTCGGG 
AAAGGGACTT CTCCTGGGGA CCGAAGCCTC CCTTACTCTG CTTTCCCGGG CGGCGACACC 
GAATCCCTGT GCGGAGACCC CGGTGTCCAG ACGAATGAGG AGCAGGAGAG GACCCGCCAC 
GACACCGCGG GCTCAGCCAT GGGTGACCCC GGAACCGAGA GCATCGCACC CGTGTGCCCT 
CCTTTACAGT GTAATCAGGA CGAAGACTCC TTGACTTTGC TCATCCAAGT GCCTGGGATT 
CTGCCACAAA CTCTTCACGG ACATCTAAGC CCCGTCGGTT ACGAATTGTG CTTCTCTACA 
CAAGACTCGG GTTATTCCTG CACTTTGCAG TTTGCTCCAG AGAATAAGTT GAGTACCAGA 
GAACCCGAGA CTAGCGTTTC TTTAAATAAT GCAGTCATAG TACTGGCAAA ATCTCCAGAG 
AGCCACGGAC TTTGGCGAGA GTGGTATTGT GGCTTGAACA AGGAGTCTTT GGAGTTGCAG 
GAATGTAGTG ATCCTGCTGG GCTTCAAGGA AAGGGAAAAG GAGTCAGAGA AGGCTGTCCT 
CTGAGTGAAG CAGAAGCCGC TGACCAGTCC GCCACCTCTC CAGCTGCTTC TGATTCAGCT 
GCAGCAGTCG AAGCACTAAA AATAAACACG CATGGTTCAG CTGTGGACCT GCAACACGGC 
TGTCCTGAGG TTCCTCACGT ACTGTCTGGA AAACCGCTGC AACCAGAGGC AAAAATGGAT 
CCTGAGTTTA TAAGAGAAAG TAGTACTACC TACTCGACTG AGGAAAAGGA AAATATAAGA 
GAGCCAGTAA TAAGCAAGGG GGAAAAAATA AATGGAGATC ACCCATCTTC ATTACTGAAC 
AAAACTGTGG TTCAAAACAT ACCTGACTTT GACACTATAA AAGAGACGAA TATGCAGGAT 
GGTAGTGTGC AGATTATTAG AGACCACACG ACTCATTGTG CATTCAGTTT TCAGAATCCC 
TTGCTATATG ATTTAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.
The Journal of cell biology204(2)203-13(2014 Jan)
Dong F,Shinohara K,Botilde Y,Nabeshima R,Asai Y,Fukumoto A,Hasegawa T,Matsuo M,Takeda H,Shiratori H,Nakamura T,Hamada H

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

The establishment of rotational polarity in the airway and ependymal cilia: analysis with a novel cilium motility mutant mouse.
American journal of physiology. Lung cellular and molecular physiology304(11)L736-45(2013 Jun)
Matsuo M,Shimada A,Koshida S,Saga Y,Takeda H

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Dnaaf2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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