Ibtk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ibtk
Entrez Gene ID	108837
Full Name	inhibitor of Bruton agammaglobulinemia tyrosine kinase
Synonyms	5430411K16Rik,AA409502,BTKI,mKIAA1417
General protein information	Preferred Names inhibitor of Bruton agammaglobulinemia tyrosine kinase Names inhibitor of Bruton tyrosine kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E3.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006510766.3	XP_006510829.1	inhibitor of Bruton tyrosine kinase isoform X2
XM_006510765.3	XP_006510828.1	inhibitor of Bruton tyrosine kinase isoform X1
NM_175371.3	NP_780580.2	inhibitor of Bruton tyrosine kinase isoform 2
XM_006510765.4	XP_006510828.1	inhibitor of Bruton tyrosine kinase isoform X1
NM_001081282.2	NP_001074751.1	inhibitor of Bruton tyrosine kinase isoform 1

Pathways from BioSystems

Homologies

	IBTK	XP_002803869.1
Bos taurus (cattle)	IBTK	NP_001192969.1
Drosophila melanogaster (fruit fly)	CG8060	NP_611117.2
Pan troglodytes (chimpanzee)	IBTK	XP_001148806.1
Xenopus tropicalis (tropical clawed frog)	ibtk	XP_002938503.2
Canis lupus familiaris (dog)	IBTK	XP_539018.2
Mus musculus (house mouse)	Ibtk	NP_001074751.1
Rattus norvegicus (Norway rat)	Ibtk	XP_006243569.1
Gallus gallus (chicken)	IBTK	XP_004940475.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009939	XP_319065.4
Homo sapiens (human)	IBTK	NP_056340.2
Danio rerio (zebrafish)	ibtk	XP_005157916.1

Gene Ontology
Bibliography

Related articles in PubMed

Btk regulation in human and mouse B cells via protein kinase C phosphorylation of IBtk?.
Janda E, Palmieri C, Pisano A, Pontoriero M, Iaccino E, Falcone C, Fiume G, Gaspari M, Nevolo M, Di Salle E, Rossi A, De Laurentiis A, Greco A, Di Napoli D, Verheij E, Britti D, Lavecchia L, Quinto I, Scala G
Blood117(24)6520-31(2011 Jun)

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization.
Yoshikawa T, Piao Y, Zhong J, Matoba R, Carter MG, Wang Y, Goldberg I, Ko MS
Gene expression patterns : GEP6(2)213-24(2006 Jan)

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
Nishimura M, Yokoi N, Miki T, Horikawa Y, Yoshioka H, Takeda J, Ohara O, Seino S
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ibtk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ibtk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu42511	NM_175371.3 Latest version!	Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu42511	XM_006510766.3 Latest version!	Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu42510	XM_006510765.4 Latest version!	Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu02023	NM_001081282.2 Latest version!	Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$790.30 $1129.00
View Old Accession Versions >>
OMu42510	XM_006510765.3	Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ibtk ( NM_175371.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780580.2 Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu42511
Clone ID Related Accession (Same CDS sequence)	XM_006510766.3 , NM_175371.3
Accession Version	NM_175371.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	inhibitor of Bruton tyrosine kinase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC155934.2. On or before Jan 13, 2018 this sequence version replaced XM_006510766.3, NM_175371.2. Transcript Variant: This variant (2) lacks an exon and uses an in-frame downstream translation start site, compared to variant 1. It encodes a protein (isoform 2) with a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC060722.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACAGCT TCCACGATGT GACCTTTCAA GTTGGCAATA GACACTTCCC CGCACATAAA 
TACATTTTGG CCGTGCGCTC TGACTTCTTC CAGAAGTTGT TTCTTTCAGA TGGCTCTTCT 
TTAGAGCTGA CGGATGTTTA CCAGAAAGAC GAAGATGCTG CTGGCTGCCA TCTCTTTGTG 
GTGGAGAAAG TCCATCCTGA CTTGTTCGAA TACCTCTTAC AGTTCATGTA CACAGATACC 
TGTGACTTGT TAACTCATGG CTTCAAACCA AGAATGATCG TGAAGAGAAA GGCAGAAGAC 
TGTGAGGGCT CCCCAGATTC TCATCTGCAC ACTGTGAATT GCCATGTGGA TGATAAACAG 
AAGTCAGCAT TTGAGGTGTA CAGAAGCAAC CAGGCCCACA CACTTAGTGA ACGGCAGAAG 
AGCAAACCCA AATCTTCTAA GAAGGGAAAA GGTGTTGGGG ACGACGACCC TGTAAGGATG 
TTGCAAAGTG TTGCTAAGAA GTTTGGCCTC AGTAATTTGA GTAGCAGGTT AGAAGGAGTC 
AGATTGGAAA ATGAAAAAAT TAATGTTATT GCGAAGAAAA CTGGCAATAA ACTGAAGCTA 
AGTCAGAAAA AATGTTCGTT TCTGTATGAT GTCACCATGA AGTCTGTGGA TGGAAAGGAG 
TTCTCCTGCC ATAAGTGTGT GCTCTGTGCG AGACTTGAGT ATTTCCACAG CATGCTTAGC 
AGATCGTGGA TTGAGGCCTC CAGCTGTGCA GCTCTGGAAA TGCCAATCCA GTCTGAGATT 
CTGAAAGTGA TTTTGGACTA CCTCTACACT GATGAGGCTG TGGTGATAAA AGAGTCTCAA 
AACGTGGACT TTGTTTGTAG TGTTCTTGTC GTGGCTGATC AGCTTCTCAT AACTCGGTTA 
AAGGAGATTT GTGAAGTAGC ATTGACTGAA AACCTTACCC TTAAGAATGC TGCGATGCTG 
CTGGAGTTTG CAGCCTTGTA CAATGCTGGG CAGTTGAAGC TCTCTTGTTT ACAGTTCATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTGG ATGTTTTAAG TGAGGATGTT 
TTGAAGGACC TTTCCATATT CTACAGAAAA ATGATTCCAG CAATGGAGAG AAGAGTCATT 
ACACCGTATC AAGATGGGCC AGATATTAGC TCTATGCAAG TAGAAGACGG AGAGGTCTTT 
TTTAAAGAAG AAATAAACAT GGAACCAAAT TACTCGGAAA CTATGTTCAA GAAGGCGAAA 
ACAAGAGCTA AAAAGAAGCC ACGGAAGCGC TCAGACAGTT CAGGAGGCTA CACCCTTTCA 
GATGTTATCC AGAGTCCACC GTCTGCAGGA TTATTAAAGT CTGCAAAGAC CAATTCTGTG 
GAGTCTCTTC CAGAATTGTT GACCTCAGAT TCGGAAGGAA GCTATGCAGG AGTGGCGAGT 
CCCAGAGACC TCCAGTCGCC CGACTTCACA GCAGGATTCC ATTCGGATAA GGTCGAGGGG 
AAAGCTAAGC CATACGTGAA TGGTATACCT CCTCCATGTA CTAGGGAAGA TGTAAAGCCG 
TGGGAGAAAT CACCAACAAC TAAGTCTGCT CCCCAGTTCA TCCCCAGTAA CAGAGTGGAC 
ACTGCAGCCT CTTCCAGTTG GCTTGCCGGC TCTTGCAGTC CTGTGAGTCC TCCTGTTGTG 
GATCTTAGAA CCATCATGGA AACAGAAGAA AACAGACAGA AATATGGCGC AGCACCAAAG 
TCAAACTTAG GAAAGATTAT TTCTCACGGA ATCAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCATTGA CCACCAAAGA AAACAATTCA GGAACGAACA GCATGGAAGC CATTTTGACA 
GCTCCTTCCA AGTCCCCCAA GCCTGCGAAT GCATGGGCAC CTCTGCACTC ACCTTTATCC 
AGGTCATTCC GGGATTTTTT ACTAGAAGAA AAAAAACCCG TTCCTGGCTA TGGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ACTGAAAACT CTCCAGCCCT AAATGTTGCA 
AGATGTTCAA CCCATGGAAC TCCAGGATTA GAAAGCAATC ATGTTTCTGA TTTTCCACTT 
CTAGACAGTC CCAACCCCTG GCAGTCTTCC TCCCTGGCGG CTTCTCCCGC AGTTGCTCCA 
GTCACATTTG CATCCATTGT AGAAGAAGAG CGGCAACAAG AAGCTGCCCT CATCAGAAGC 
CGGGAGAAAC CCTTGGCTCT CATTCAGGTC GAGGAGCATG CAATACAAGA CTTGCTGGTT 
TTCTATGAGG CATTTGGCAA CCCCGAGGAG TTTGTCGTCG TTGAAAGGGC ACCACAGGGG 
CCTCTGGCAG TGCCTATGTG GAACAAGCAT GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780580.2
CDS	2071..4449
Translation	MDSFHDVTFQVGNRHFPAHKYILAVRSDFFQKLFLSDGSSLELTDVYQKDEDAAGCHLFVVEKVHPDLFEYLLQFMYTDTCDLLTHGFKPRMIVKRKAEDCEGSPDSHLHTVNCHVDDKQKSAFEVYRSNQAHTLSERQKSKPKSSKKGKGVGDDDPVRMLQSVAKKFGLSNLSSRLEGVRLENEKINVIAKKTGNKLKLSQKKCSFLYDVTMKSVDGKEFSCHKCVLCARLEYFHSMLSRSWIEASSCAALEMPIQSEILKVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKEICEVALTENLTLKNAAMLLEFAALYNAGQLKLSCLQFIGLNMAALLEARSLDVLSEDVLKDLSIFYRKMIPAMERRVITPYQDGPDISSMQVEDGEVFFKEEINMEPNYSETMFKKAKTRAKKKPRKRSDSSGGYTLSDVIQSPPSAGLLKSAKTNSVESLPELLTSDSEGSYAGVASPRDLQSPDFTAGFHSDKVEGKAKPYVNGIPPPCTREDVKPWEKSPTTKSAPQFIPSNRVDTAASSSWLAGSCSPVSPPVVDLRTIMETEENRQKYGAAPKSNLGKIISHGIKLSQKQRKMIALTTKENNSGTNSMEAILTAPSKSPKPANAWAPLHSPLSRSFRDFLLEEKKPVPGYGSGDHVKKVCFKGTENSPALNVARCSTHGTPGLESNHVSDFPLLDSPNPWQSSSLAASPAVAPVTFASIVEEERQQEAALIRSREKPLALIQVEEHAIQDLLVFYEAFGNPEEFVVVERAPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	NM_175371.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175371.3

ORF Insert Sequence:

ATGGACAGCT TCCACGATGT GACCTTTCAA GTTGGCAATA GACACTTCCC CGCACATAAA 
TACATTTTGG CCGTGCGCTC TGACTTCTTC CAGAAGTTGT TTCTTTCAGA TGGCTCTTCT 
TTAGAGCTGA CGGATGTTTA CCAGAAAGAC GAAGATGCTG CTGGCTGCCA TCTCTTTGTG 
GTGGAGAAAG TCCATCCTGA CTTGTTCGAA TACCTCTTAC AGTTCATGTA CACAGATACC 
TGTGACTTGT TAACTCATGG CTTCAAACCA AGAATGATCG TGAAGAGAAA GGCAGAAGAC 
TGTGAGGGCT CCCCAGATTC TCATCTGCAC ACTGTGAATT GCCATGTGGA TGATAAACAG 
AAGTCAGCAT TTGAGGTGTA CAGAAGCAAC CAGGCCCACA CACTTAGTGA ACGGCAGAAG 
AGCAAACCCA AATCTTCTAA GAAGGGAAAA GGTGTTGGGG ACGACGACCC TGTAAGGATG 
TTGCAAAGTG TTGCTAAGAA GTTTGGCCTC AGTAATTTGA GTAGCAGGTT AGAAGGAGTC 
AGATTGGAAA ATGAAAAAAT TAATGTTATT GCGAAGAAAA CTGGCAATAA ACTGAAGCTA 
AGTCAGAAAA AATGTTCGTT TCTGTATGAT GTCACCATGA AGTCTGTGGA TGGAAAGGAG 
TTCTCCTGCC ATAAGTGTGT GCTCTGTGCG AGACTTGAGT ATTTCCACAG CATGCTTAGC 
AGATCGTGGA TTGAGGCCTC CAGCTGTGCA GCTCTGGAAA TGCCAATCCA GTCTGAGATT 
CTGAAAGTGA TTTTGGACTA CCTCTACACT GATGAGGCTG TGGTGATAAA AGAGTCTCAA 
AACGTGGACT TTGTTTGTAG TGTTCTTGTC GTGGCTGATC AGCTTCTCAT AACTCGGTTA 
AAGGAGATTT GTGAAGTAGC ATTGACTGAA AACCTTACCC TTAAGAATGC TGCGATGCTG 
CTGGAGTTTG CAGCCTTGTA CAATGCTGGG CAGTTGAAGC TCTCTTGTTT ACAGTTCATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTGG ATGTTTTAAG TGAGGATGTT 
TTGAAGGACC TTTCCATATT CTACAGAAAA ATGATTCCAG CAATGGAGAG AAGAGTCATT 
ACACCGTATC AAGATGGGCC AGATATTAGC TCTATGCAAG TAGAAGACGG AGAGGTCTTT 
TTTAAAGAAG AAATAAACAT GGAACCAAAT TACTCGGAAA CTATGTTCAA GAAGGCGAAA 
ACAAGAGCTA AAAAGAAGCC ACGGAAGCGC TCAGACAGTT CAGGAGGCTA CACCCTTTCA 
GATGTTATCC AGAGTCCACC GTCTGCAGGA TTATTAAAGT CTGCAAAGAC CAATTCTGTG 
GAGTCTCTTC CAGAATTGTT GACCTCAGAT TCGGAAGGAA GCTATGCAGG AGTGGCGAGT 
CCCAGAGACC TCCAGTCGCC CGACTTCACA GCAGGATTCC ATTCGGATAA GGTCGAGGGG 
AAAGCTAAGC CATACGTGAA TGGTATACCT CCTCCATGTA CTAGGGAAGA TGTAAAGCCG 
TGGGAGAAAT CACCAACAAC TAAGTCTGCT CCCCAGTTCA TCCCCAGTAA CAGAGTGGAC 
ACTGCAGCCT CTTCCAGTTG GCTTGCCGGC TCTTGCAGTC CTGTGAGTCC TCCTGTTGTG 
GATCTTAGAA CCATCATGGA AACAGAAGAA AACAGACAGA AATATGGCGC AGCACCAAAG 
TCAAACTTAG GAAAGATTAT TTCTCACGGA ATCAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCATTGA CCACCAAAGA AAACAATTCA GGAACGAACA GCATGGAAGC CATTTTGACA 
GCTCCTTCCA AGTCCCCCAA GCCTGCGAAT GCATGGGCAC CTCTGCACTC ACCTTTATCC 
AGGTCATTCC GGGATTTTTT ACTAGAAGAA AAAAAACCCG TTCCTGGCTA TGGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ACTGAAAACT CTCCAGCCCT AAATGTTGCA 
AGATGTTCAA CCCATGGAAC TCCAGGATTA GAAAGCAATC ATGTTTCTGA TTTTCCACTT 
CTAGACAGTC CCAACCCCTG GCAGTCTTCC TCCCTGGCGG CTTCTCCCGC AGTTGCTCCA 
GTCACATTTG CATCCATTGT AGAAGAAGAG CGGCAACAAG AAGCTGCCCT CATCAGAAGC 
CGGGAGAAAC CCTTGGCTCT CATTCAGGTC GAGGAGCATG CAATACAAGA CTTGCTGGTT 
TTCTATGAGG CATTTGGCAA CCCCGAGGAG TTTGTCGTCG TTGAAAGGGC ACCACAGGGG 
CCTCTGGCAG TGCCTATGTG GAACAAGCAT GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ibtk ( XM_006510766.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510829.1 Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu42511
Clone ID Related Accession (Same CDS sequence)	XM_006510766.3 , NM_175371.3
Accession Version	XM_006510766.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inhibitor of Bruton tyrosine kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCT TCCACGATGT GACCTTTCAA GTTGGCAATA GACACTTCCC CGCACATAAA 
TACATTTTGG CCGTGCGCTC TGACTTCTTC CAGAAGTTGT TTCTTTCAGA TGGCTCTTCT 
TTAGAGCTGA CGGATGTTTA CCAGAAAGAC GAAGATGCTG CTGGCTGCCA TCTCTTTGTG 
GTGGAGAAAG TCCATCCTGA CTTGTTCGAA TACCTCTTAC AGTTCATGTA CACAGATACC 
TGTGACTTGT TAACTCATGG CTTCAAACCA AGAATGATCG TGAAGAGAAA GGCAGAAGAC 
TGTGAGGGCT CCCCAGATTC TCATCTGCAC ACTGTGAATT GCCATGTGGA TGATAAACAG 
AAGTCAGCAT TTGAGGTGTA CAGAAGCAAC CAGGCCCACA CACTTAGTGA ACGGCAGAAG 
AGCAAACCCA AATCTTCTAA GAAGGGAAAA GGTGTTGGGG ACGACGACCC TGTAAGGATG 
TTGCAAAGTG TTGCTAAGAA GTTTGGCCTC AGTAATTTGA GTAGCAGGTT AGAAGGAGTC 
AGATTGGAAA ATGAAAAAAT TAATGTTATT GCGAAGAAAA CTGGCAATAA ACTGAAGCTA 
AGTCAGAAAA AATGTTCGTT TCTGTATGAT GTCACCATGA AGTCTGTGGA TGGAAAGGAG 
TTCTCCTGCC ATAAGTGTGT GCTCTGTGCG AGACTTGAGT ATTTCCACAG CATGCTTAGC 
AGATCGTGGA TTGAGGCCTC CAGCTGTGCA GCTCTGGAAA TGCCAATCCA GTCTGAGATT 
CTGAAAGTGA TTTTGGACTA CCTCTACACT GATGAGGCTG TGGTGATAAA AGAGTCTCAA 
AACGTGGACT TTGTTTGTAG TGTTCTTGTC GTGGCTGATC AGCTTCTCAT AACTCGGTTA 
AAGGAGATTT GTGAAGTAGC ATTGACTGAA AACCTTACCC TTAAGAATGC TGCGATGCTG 
CTGGAGTTTG CAGCCTTGTA CAATGCTGGG CAGTTGAAGC TCTCTTGTTT ACAGTTCATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTGG ATGTTTTAAG TGAGGATGTT 
TTGAAGGACC TTTCCATATT CTACAGAAAA ATGATTCCAG CAATGGAGAG AAGAGTCATT 
ACACCGTATC AAGATGGGCC AGATATTAGC TCTATGCAAG TAGAAGACGG AGAGGTCTTT 
TTTAAAGAAG AAATAAACAT GGAACCAAAT TACTCGGAAA CTATGTTCAA GAAGGCGAAA 
ACAAGAGCTA AAAAGAAGCC ACGGAAGCGC TCAGACAGTT CAGGAGGCTA CACCCTTTCA 
GATGTTATCC AGAGTCCACC GTCTGCAGGA TTATTAAAGT CTGCAAAGAC CAATTCTGTG 
GAGTCTCTTC CAGAATTGTT GACCTCAGAT TCGGAAGGAA GCTATGCAGG AGTGGCGAGT 
CCCAGAGACC TCCAGTCGCC CGACTTCACA GCAGGATTCC ATTCGGATAA GGTCGAGGGG 
AAAGCTAAGC CATACGTGAA TGGTATACCT CCTCCATGTA CTAGGGAAGA TGTAAAGCCG 
TGGGAGAAAT CACCAACAAC TAAGTCTGCT CCCCAGTTCA TCCCCAGTAA CAGAGTGGAC 
ACTGCAGCCT CTTCCAGTTG GCTTGCCGGC TCTTGCAGTC CTGTGAGTCC TCCTGTTGTG 
GATCTTAGAA CCATCATGGA AACAGAAGAA AACAGACAGA AATATGGCGC AGCACCAAAG 
TCAAACTTAG GAAAGATTAT TTCTCACGGA ATCAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCATTGA CCACCAAAGA AAACAATTCA GGAACGAACA GCATGGAAGC CATTTTGACA 
GCTCCTTCCA AGTCCCCCAA GCCTGCGAAT GCATGGGCAC CTCTGCACTC ACCTTTATCC 
AGGTCATTCC GGGATTTTTT ACTAGAAGAA AAAAAACCCG TTCCTGGCTA TGGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ACTGAAAACT CTCCAGCCCT AAATGTTGCA 
AGATGTTCAA CCCATGGAAC TCCAGGATTA GAAAGCAATC ATGTTTCTGA TTTTCCACTT 
CTAGACAGTC CCAACCCCTG GCAGTCTTCC TCCCTGGCGG CTTCTCCCGC AGTTGCTCCA 
GTCACATTTG CATCCATTGT AGAAGAAGAG CGGCAACAAG AAGCTGCCCT CATCAGAAGC 
CGGGAGAAAC CCTTGGCTCT CATTCAGGTC GAGGAGCATG CAATACAAGA CTTGCTGGTT 
TTCTATGAGG CATTTGGCAA CCCCGAGGAG TTTGTCGTCG TTGAAAGGGC ACCACAGGGG 
CCTCTGGCAG TGCCTATGTG GAACAAGCAT GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510829.1
CDS	2042..4420
Translation	MDSFHDVTFQVGNRHFPAHKYILAVRSDFFQKLFLSDGSSLELTDVYQKDEDAAGCHLFVVEKVHPDLFEYLLQFMYTDTCDLLTHGFKPRMIVKRKAEDCEGSPDSHLHTVNCHVDDKQKSAFEVYRSNQAHTLSERQKSKPKSSKKGKGVGDDDPVRMLQSVAKKFGLSNLSSRLEGVRLENEKINVIAKKTGNKLKLSQKKCSFLYDVTMKSVDGKEFSCHKCVLCARLEYFHSMLSRSWIEASSCAALEMPIQSEILKVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKEICEVALTENLTLKNAAMLLEFAALYNAGQLKLSCLQFIGLNMAALLEARSLDVLSEDVLKDLSIFYRKMIPAMERRVITPYQDGPDISSMQVEDGEVFFKEEINMEPNYSETMFKKAKTRAKKKPRKRSDSSGGYTLSDVIQSPPSAGLLKSAKTNSVESLPELLTSDSEGSYAGVASPRDLQSPDFTAGFHSDKVEGKAKPYVNGIPPPCTREDVKPWEKSPTTKSAPQFIPSNRVDTAASSSWLAGSCSPVSPPVVDLRTIMETEENRQKYGAAPKSNLGKIISHGIKLSQKQRKMIALTTKENNSGTNSMEAILTAPSKSPKPANAWAPLHSPLSRSFRDFLLEEKKPVPGYGSGDHVKKVCFKGTENSPALNVARCSTHGTPGLESNHVSDFPLLDSPNPWQSSSLAASPAVAPVTFASIVEEERQQEAALIRSREKPLALIQVEEHAIQDLLVFYEAFGNPEEFVVVERAPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_006510766.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510766.3

ORF Insert Sequence:

ATGGACAGCT TCCACGATGT GACCTTTCAA GTTGGCAATA GACACTTCCC CGCACATAAA 
TACATTTTGG CCGTGCGCTC TGACTTCTTC CAGAAGTTGT TTCTTTCAGA TGGCTCTTCT 
TTAGAGCTGA CGGATGTTTA CCAGAAAGAC GAAGATGCTG CTGGCTGCCA TCTCTTTGTG 
GTGGAGAAAG TCCATCCTGA CTTGTTCGAA TACCTCTTAC AGTTCATGTA CACAGATACC 
TGTGACTTGT TAACTCATGG CTTCAAACCA AGAATGATCG TGAAGAGAAA GGCAGAAGAC 
TGTGAGGGCT CCCCAGATTC TCATCTGCAC ACTGTGAATT GCCATGTGGA TGATAAACAG 
AAGTCAGCAT TTGAGGTGTA CAGAAGCAAC CAGGCCCACA CACTTAGTGA ACGGCAGAAG 
AGCAAACCCA AATCTTCTAA GAAGGGAAAA GGTGTTGGGG ACGACGACCC TGTAAGGATG 
TTGCAAAGTG TTGCTAAGAA GTTTGGCCTC AGTAATTTGA GTAGCAGGTT AGAAGGAGTC 
AGATTGGAAA ATGAAAAAAT TAATGTTATT GCGAAGAAAA CTGGCAATAA ACTGAAGCTA 
AGTCAGAAAA AATGTTCGTT TCTGTATGAT GTCACCATGA AGTCTGTGGA TGGAAAGGAG 
TTCTCCTGCC ATAAGTGTGT GCTCTGTGCG AGACTTGAGT ATTTCCACAG CATGCTTAGC 
AGATCGTGGA TTGAGGCCTC CAGCTGTGCA GCTCTGGAAA TGCCAATCCA GTCTGAGATT 
CTGAAAGTGA TTTTGGACTA CCTCTACACT GATGAGGCTG TGGTGATAAA AGAGTCTCAA 
AACGTGGACT TTGTTTGTAG TGTTCTTGTC GTGGCTGATC AGCTTCTCAT AACTCGGTTA 
AAGGAGATTT GTGAAGTAGC ATTGACTGAA AACCTTACCC TTAAGAATGC TGCGATGCTG 
CTGGAGTTTG CAGCCTTGTA CAATGCTGGG CAGTTGAAGC TCTCTTGTTT ACAGTTCATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTGG ATGTTTTAAG TGAGGATGTT 
TTGAAGGACC TTTCCATATT CTACAGAAAA ATGATTCCAG CAATGGAGAG AAGAGTCATT 
ACACCGTATC AAGATGGGCC AGATATTAGC TCTATGCAAG TAGAAGACGG AGAGGTCTTT 
TTTAAAGAAG AAATAAACAT GGAACCAAAT TACTCGGAAA CTATGTTCAA GAAGGCGAAA 
ACAAGAGCTA AAAAGAAGCC ACGGAAGCGC TCAGACAGTT CAGGAGGCTA CACCCTTTCA 
GATGTTATCC AGAGTCCACC GTCTGCAGGA TTATTAAAGT CTGCAAAGAC CAATTCTGTG 
GAGTCTCTTC CAGAATTGTT GACCTCAGAT TCGGAAGGAA GCTATGCAGG AGTGGCGAGT 
CCCAGAGACC TCCAGTCGCC CGACTTCACA GCAGGATTCC ATTCGGATAA GGTCGAGGGG 
AAAGCTAAGC CATACGTGAA TGGTATACCT CCTCCATGTA CTAGGGAAGA TGTAAAGCCG 
TGGGAGAAAT CACCAACAAC TAAGTCTGCT CCCCAGTTCA TCCCCAGTAA CAGAGTGGAC 
ACTGCAGCCT CTTCCAGTTG GCTTGCCGGC TCTTGCAGTC CTGTGAGTCC TCCTGTTGTG 
GATCTTAGAA CCATCATGGA AACAGAAGAA AACAGACAGA AATATGGCGC AGCACCAAAG 
TCAAACTTAG GAAAGATTAT TTCTCACGGA ATCAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCATTGA CCACCAAAGA AAACAATTCA GGAACGAACA GCATGGAAGC CATTTTGACA 
GCTCCTTCCA AGTCCCCCAA GCCTGCGAAT GCATGGGCAC CTCTGCACTC ACCTTTATCC 
AGGTCATTCC GGGATTTTTT ACTAGAAGAA AAAAAACCCG TTCCTGGCTA TGGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ACTGAAAACT CTCCAGCCCT AAATGTTGCA 
AGATGTTCAA CCCATGGAAC TCCAGGATTA GAAAGCAATC ATGTTTCTGA TTTTCCACTT 
CTAGACAGTC CCAACCCCTG GCAGTCTTCC TCCCTGGCGG CTTCTCCCGC AGTTGCTCCA 
GTCACATTTG CATCCATTGT AGAAGAAGAG CGGCAACAAG AAGCTGCCCT CATCAGAAGC 
CGGGAGAAAC CCTTGGCTCT CATTCAGGTC GAGGAGCATG CAATACAAGA CTTGCTGGTT 
TTCTATGAGG CATTTGGCAA CCCCGAGGAG TTTGTCGTCG TTGAAAGGGC ACCACAGGGG 
CCTCTGGCAG TGCCTATGTG GAACAAGCAT GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ibtk ( XM_006510765.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510828.1 Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant X1, mRNA.

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CloneID	OMu42510
Clone ID Related Accession (Same CDS sequence)	XM_006510765.3 , XM_006510765.4
Accession Version	XM_006510765.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510765.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG CCACCCCCGA CTGCACGTCA AAATGCAGAT CCCTGAAGCA TGCGCTGGAT 
GTCCTTTCTG TGGTGACAAA GGGGAGTGAG AGCCAGATCA AGTCCTTCCT CGCCAGGTAC 
TGCTACAATG CTGCAACTGT CAAGGATGCC TTTGGCAGGA ACGCCGGCCA CCTGGCTTCC 
TCCTGCGGGA AGAAAGGAGT GTTGGACTGG CTGATTGAGA AAGGAGTGGA CTTGCTGGTG 
AAAGACAAGG AGTCCGGCTG GACAGCACTG CACAGAAGCG TTTTTTATGG ACATATCGAT 
TGTGTTTGGT CTCTGTTGAA GCATGGTGTT AGCTTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTTAT GAAGGACAGG CCAACTCATG TTGTATTTAA GGACACTGAT 
CCCACAGAAG TCTATACTTG GGGAGATAAC ACAAACTTCA CTCTGGGCCA CGGAAGCCAG 
AATAGCAAAC ATCACCCCGA GCTGCTGGAC CTGTTCTCCA GGAGTGGGGT TTATGTCAAG 
CAGGTGGTGC TTTGCAAATT TCATTCGGTG TTTCTGTCCC AGAAAGGGCA GGTTTACACC 
TGTGGCCATG GTCGCGGAGG ACGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGGCTTGTGG AAGGACTGTC TGGTCACAAT TGTTCGCAAG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTGT TAACTGATGA TGGATGTGTC TATACATTTG GTCTCAACAT GTTTCACCAA 
TTAGGAATTA TTCCTCCACC TGCTAGTTGT AATGTACCCA GACAGATACA GGCAAAATAT 
CTGAAAGGAA GGACTATCAT TGGAGTAGCA GCGGGCAGGT TTCATACGGT GCTGTGGACT 
CGAGAAGCCG TTTACACACT GGGACTGAAT GGTGGACAGT TGGGTCACTT ACTGGATCCC 
AATGGTGAAA AGTGTGTCAC AACCCCTCGC CAAGTCTCTG CCCTTCATCA CAAGGATATC 
GCTGTGTCTT TGGTAGCTGC AAGTGATGGT GCCACAGTCT GTGTCACCAC AAGGGGAGAC 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCAA CTAAACAACT GAACTTGAAA 
AAGGTTCTTG TCTCTGGAGG TTGTATGGAA TACAAGGTTG ATCCTGAACA TTTGACAGAA 
AATGGAGGTC AGAAAATATG TGTCCTCGCT ATGGATGGAG CTGGAAGGGT GTTTTGCTGG 
AGATCAATCA GCAGTTCTCT GAAGCAGTGC CGATGGGCCT ATCCTCGCCA GGTGTCAATC 
TCCGATATTG CTTTAAATAG AAATGAAATT CTGTTTGTCA CACAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGA CAAAAGAAAG AATTCTGAAA AGAAAGACAT TTTACCAAAC 
CTCCATCATT CCTCATCAGA TGTGTCTTGT GTCCCTGATA CAAATAGTGT GTATGAAAGA 
ATTCGACTTG AGAAACTTCC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGGTGCAACT TTGCAATCCT ACAGTCAGAC CCTAAAACAA GCCTTTATGA GATTCCAGTT 
GTGTCTTCAT CATCTTTTTT TGAAGAATTT GGCAAGCTCC TGAGGGAGAC AGATGAAATG 
GACAGCTTCC ACGATGTGAC CTTTCAAGTT GGCAATAGAC ACTTCCCCGC ACATAAATAC 
ATTTTGGCCG TGCGCTCTGA CTTCTTCCAG AAGTTGTTTC TTTCAGATGG CTCTTCTTTA 
GAGCTGACGG ATGTTTACCA GAAAGACGAA GATGCTGCTG GCTGCCATCT CTTTGTGGTG 
GAGAAAGTCC ATCCTGACTT GTTCGAATAC CTCTTACAGT TCATGTACAC AGATACCTGT 
GACTTGTTAA CTCATGGCTT CAAACCAAGA ATGATCGTGA AGAGAAAGGC AGAAGACTGT 
GAGGGCTCCC CAGATTCTCA TCTGCACACT GTGAATTGCC ATGTGGATGA TAAACAGAAG 
TCAGCATTTG AGGTGTACAG AAGCAACCAG GCCCACACAC TTAGTGAACG GCAGAAGAGC 
AAACCCAAAT CTTCTAAGAA GGGAAAAGGT GTTGGGGACG ACGACCCTGT AAGGATGTTG 
CAAAGTGTTG CTAAGAAGTT TGGCCTCAGT AATTTGAGTA GCAGGTTAGA AGGAGTCAGA 
TTGGAAAATG AAAAAATTAA TGTTATTGCG AAGAAAACTG GCAATAAACT GAAGCTAAGT 
CAGAAAAAAT GTTCGTTTCT GTATGATGTC ACCATGAAGT CTGTGGATGG AAAGGAGTTC 
TCCTGCCATA AGTGTGTGCT CTGTGCGAGA CTTGAGTATT TCCACAGCAT GCTTAGCAGA 
TCGTGGATTG AGGCCTCCAG CTGTGCAGCT CTGGAAATGC CAATCCAGTC TGAGATTCTG 
AAAGTGATTT TGGACTACCT CTACACTGAT GAGGCTGTGG TGATAAAAGA GTCTCAAAAC 
GTGGACTTTG TTTGTAGTGT TCTTGTCGTG GCTGATCAGC TTCTCATAAC TCGGTTAAAG 
GAGATTTGTG AAGTAGCATT GACTGAAAAC CTTACCCTTA AGAATGCTGC GATGCTGCTG 
GAGTTTGCAG CCTTGTACAA TGCTGGGCAG TTGAAGCTCT CTTGTTTACA GTTCATAGGA 
CTGAATATGG CAGCTTTACT TGAAGCAAGG TCTCTGGATG TTTTAAGTGA GGATGTTTTG 
AAGGACCTTT CCATATTCTA CAGAAAAATG ATTCCAGCAA TGGAGAGAAG AGTCATTACA 
CCGTATCAAG ATGGGCCAGA TATTAGCTCT ATGCAAGTAG AAGACGGAGA GGTCTTTTTT 
AAAGAAGAAA TAAACATGGA ACCAAATTAC TCGGAAACTA TGTTCAAGAA GGCGAAAACA 
AGAGCTAAAA AGAAGCCACG GAAGCGCTCA GACAGTTCAG GAGGCTACAC CCTTTCAGAT 
GTTATCCAGA GTCCACCGTC TGCAGGATTA TTAAAGTCTG CAAAGACCAA TTCTGTGGAG 
TCTCTTCCAG AATTGTTGAC CTCAGATTCG GAAGGAAGCT ATGCAGGAGT GGCGAGTCCC 
AGAGACCTCC AGTCGCCCGA CTTCACAGCA GGATTCCATT CGGATAAGGT CGAGGGGAAA 
GCTAAGCCAT ACGTGAATGG TATACCTCCT CCATGTACTA GGGAAGATGT AAAGCCGTGG 
GAGAAATCAC CAACAACTAA GTCTGCTCCC CAGTTCATCC CCAGTAACAG AGTGGACACT 
GCAGCCTCTT CCAGTTGGCT TGCCGGCTCT TGCAGTCCTG TGAGTCCTCC TGTTGTGGAT 
CTTAGAACCA TCATGGAAAC AGAAGAAAAC AGACAGAAAT ATGGCGCAGC ACCAAAGTCA 
AACTTAGGAA AGATTATTTC TCACGGAATC AAACTTTCTC AGAAGCAACG GAAAATGATT 
GCATTGACCA CCAAAGAAAA CAATTCAGGA ACGAACAGCA TGGAAGCCAT TTTGACAGCT 
CCTTCCAAGT CCCCCAAGCC TGCGAATGCA TGGGCACCTC TGCACTCACC TTTATCCAGG 
TCATTCCGGG ATTTTTTACT AGAAGAAAAA AAACCCGTTC CTGGCTATGG TTCAGGAGAT 
CATGTCAAAA AAGTTTGTTT TAAAGGAACT GAAAACTCTC CAGCCCTAAA TGTTGCAAGA 
TGTTCAACCC ATGGAACTCC AGGATTAGAA AGCAATCATG TTTCTGATTT TCCACTTCTA 
GACAGTCCCA ACCCCTGGCA GTCTTCCTCC CTGGCGGCTT CTCCCGCAGT TGCTCCAGTC 
ACATTTGCAT CCATTGTAGA AGAAGAGCGG CAACAAGAAG CTGCCCTCAT CAGAAGCCGG 
GAGAAACCCT TGGCTCTCAT TCAGGTCGAG GAGCATGCAA TACAAGACTT GCTGGTTTTC 
TATGAGGCAT TTGGCAACCC CGAGGAGTTT GTCGTCGTTG AAAGGGCACC ACAGGGGCCT 
CTGGCAGTGC CTATGTGGAA CAAGCATGGC TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510828.1
CDS	1938..5993
Translation	MDAATPDCTSKCRSLKHALDVLSVVTKGSESQIKSFLARYCYNAATVKDAFGRNAGHLASSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSVFYGHIDCVWSLLKHGVSLYMQDKEGLSPLDLLMKDRPTHVVFKDTDPTEVYTWGDNTNFTLGHGSQNSKHHPELLDLFSRSGVYVKQVVLCKFHSVFLSQKGQVYTCGHGRGGRLGHGDEQTCLVPRLVEGLSGHNCSQVAAAKDHTVVLTDDGCVYTFGLNMFHQLGIIPPPASCNVPRQIQAKYLKGRTIIGVAAGRFHTVLWTREAVYTLGLNGGQLGHLLDPNGEKCVTTPRQVSALHHKDIAVSLVAASDGATVCVTTRGDIYLLADYQCKKMATKQLNLKKVLVSGGCMEYKVDPEHLTENGGQKICVLAMDGAGRVFCWRSISSSLKQCRWAYPRQVSISDIALNRNEILFVTQDGEGFKGKWFEDKRKNSEKKDILPNLHHSSSDVSCVPDTNSVYERIRLEKLPFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPVVSSSSFFEEFGKLLRETDEMDSFHDVTFQVGNRHFPAHKYILAVRSDFFQKLFLSDGSSLELTDVYQKDEDAAGCHLFVVEKVHPDLFEYLLQFMYTDTCDLLTHGFKPRMIVKRKAEDCEGSPDSHLHTVNCHVDDKQKSAFEVYRSNQAHTLSERQKSKPKSSKKGKGVGDDDPVRMLQSVAKKFGLSNLSSRLEGVRLENEKINVIAKKTGNKLKLSQKKCSFLYDVTMKSVDGKEFSCHKCVLCARLEYFHSMLSRSWIEASSCAALEMPIQSEILKVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKEICEVALTENLTLKNAAMLLEFAALYNAGQLKLSCLQFIGLNMAALLEARSLDVLSEDVLKDLSIFYRKMIPAMERRVITPYQDGPDISSMQVEDGEVFFKEEINMEPNYSETMFKKAKTRAKKKPRKRSDSSGGYTLSDVIQSPPSAGLLKSAKTNSVESLPELLTSDSEGSYAGVASPRDLQSPDFTAGFHSDKVEGKAKPYVNGIPPPCTREDVKPWEKSPTTKSAPQFIPSNRVDTAASSSWLAGSCSPVSPPVVDLRTIMETEENRQKYGAAPKSNLGKIISHGIKLSQKQRKMIALTTKENNSGTNSMEAILTAPSKSPKPANAWAPLHSPLSRSFRDFLLEEKKPVPGYGSGDHVKKVCFKGTENSPALNVARCSTHGTPGLESNHVSDFPLLDSPNPWQSSSLAASPAVAPVTFASIVEEERQQEAALIRSREKPLALIQVEEHAIQDLLVFYEAFGNPEEFVVVERAPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_006510765.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510765.4

ORF Insert Sequence:

ATGGATGCTG CCACCCCCGA CTGCACGTCA AAATGCAGAT CCCTGAAGCA TGCGCTGGAT 
GTCCTTTCTG TGGTGACAAA GGGGAGTGAG AGCCAGATCA AGTCCTTCCT CGCCAGGTAC 
TGCTACAATG CTGCAACTGT CAAGGATGCC TTTGGCAGGA ACGCCGGCCA CCTGGCTTCC 
TCCTGCGGGA AGAAAGGAGT GTTGGACTGG CTGATTGAGA AAGGAGTGGA CTTGCTGGTG 
AAAGACAAGG AGTCCGGCTG GACAGCACTG CACAGAAGCG TTTTTTATGG ACATATCGAT 
TGTGTTTGGT CTCTGTTGAA GCATGGTGTT AGCTTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTTAT GAAGGACAGG CCAACTCATG TTGTATTTAA GGACACTGAT 
CCCACAGAAG TCTATACTTG GGGAGATAAC ACAAACTTCA CTCTGGGCCA CGGAAGCCAG 
AATAGCAAAC ATCACCCCGA GCTGCTGGAC CTGTTCTCCA GGAGTGGGGT TTATGTCAAG 
CAGGTGGTGC TTTGCAAATT TCATTCGGTG TTTCTGTCCC AGAAAGGGCA GGTTTACACC 
TGTGGCCATG GTCGCGGAGG ACGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGGCTTGTGG AAGGACTGTC TGGTCACAAT TGTTCGCAAG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTGT TAACTGATGA TGGATGTGTC TATACATTTG GTCTCAACAT GTTTCACCAA 
TTAGGAATTA TTCCTCCACC TGCTAGTTGT AATGTACCCA GACAGATACA GGCAAAATAT 
CTGAAAGGAA GGACTATCAT TGGAGTAGCA GCGGGCAGGT TTCATACGGT GCTGTGGACT 
CGAGAAGCCG TTTACACACT GGGACTGAAT GGTGGACAGT TGGGTCACTT ACTGGATCCC 
AATGGTGAAA AGTGTGTCAC AACCCCTCGC CAAGTCTCTG CCCTTCATCA CAAGGATATC 
GCTGTGTCTT TGGTAGCTGC AAGTGATGGT GCCACAGTCT GTGTCACCAC AAGGGGAGAC 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCAA CTAAACAACT GAACTTGAAA 
AAGGTTCTTG TCTCTGGAGG TTGTATGGAA TACAAGGTTG ATCCTGAACA TTTGACAGAA 
AATGGAGGTC AGAAAATATG TGTCCTCGCT ATGGATGGAG CTGGAAGGGT GTTTTGCTGG 
AGATCAATCA GCAGTTCTCT GAAGCAGTGC CGATGGGCCT ATCCTCGCCA GGTGTCAATC 
TCCGATATTG CTTTAAATAG AAATGAAATT CTGTTTGTCA CACAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGA CAAAAGAAAG AATTCTGAAA AGAAAGACAT TTTACCAAAC 
CTCCATCATT CCTCATCAGA TGTGTCTTGT GTCCCTGATA CAAATAGTGT GTATGAAAGA 
ATTCGACTTG AGAAACTTCC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGGTGCAACT TTGCAATCCT ACAGTCAGAC CCTAAAACAA GCCTTTATGA GATTCCAGTT 
GTGTCTTCAT CATCTTTTTT TGAAGAATTT GGCAAGCTCC TGAGGGAGAC AGATGAAATG 
GACAGCTTCC ACGATGTGAC CTTTCAAGTT GGCAATAGAC ACTTCCCCGC ACATAAATAC 
ATTTTGGCCG TGCGCTCTGA CTTCTTCCAG AAGTTGTTTC TTTCAGATGG CTCTTCTTTA 
GAGCTGACGG ATGTTTACCA GAAAGACGAA GATGCTGCTG GCTGCCATCT CTTTGTGGTG 
GAGAAAGTCC ATCCTGACTT GTTCGAATAC CTCTTACAGT TCATGTACAC AGATACCTGT 
GACTTGTTAA CTCATGGCTT CAAACCAAGA ATGATCGTGA AGAGAAAGGC AGAAGACTGT 
GAGGGCTCCC CAGATTCTCA TCTGCACACT GTGAATTGCC ATGTGGATGA TAAACAGAAG 
TCAGCATTTG AGGTGTACAG AAGCAACCAG GCCCACACAC TTAGTGAACG GCAGAAGAGC 
AAACCCAAAT CTTCTAAGAA GGGAAAAGGT GTTGGGGACG ACGACCCTGT AAGGATGTTG 
CAAAGTGTTG CTAAGAAGTT TGGCCTCAGT AATTTGAGTA GCAGGTTAGA AGGAGTCAGA 
TTGGAAAATG AAAAAATTAA TGTTATTGCG AAGAAAACTG GCAATAAACT GAAGCTAAGT 
CAGAAAAAAT GTTCGTTTCT GTATGATGTC ACCATGAAGT CTGTGGATGG AAAGGAGTTC 
TCCTGCCATA AGTGTGTGCT CTGTGCGAGA CTTGAGTATT TCCACAGCAT GCTTAGCAGA 
TCGTGGATTG AGGCCTCCAG CTGTGCAGCT CTGGAAATGC CAATCCAGTC TGAGATTCTG 
AAAGTGATTT TGGACTACCT CTACACTGAT GAGGCTGTGG TGATAAAAGA GTCTCAAAAC 
GTGGACTTTG TTTGTAGTGT TCTTGTCGTG GCTGATCAGC TTCTCATAAC TCGGTTAAAG 
GAGATTTGTG AAGTAGCATT GACTGAAAAC CTTACCCTTA AGAATGCTGC GATGCTGCTG 
GAGTTTGCAG CCTTGTACAA TGCTGGGCAG TTGAAGCTCT CTTGTTTACA GTTCATAGGA 
CTGAATATGG CAGCTTTACT TGAAGCAAGG TCTCTGGATG TTTTAAGTGA GGATGTTTTG 
AAGGACCTTT CCATATTCTA CAGAAAAATG ATTCCAGCAA TGGAGAGAAG AGTCATTACA 
CCGTATCAAG ATGGGCCAGA TATTAGCTCT ATGCAAGTAG AAGACGGAGA GGTCTTTTTT 
AAAGAAGAAA TAAACATGGA ACCAAATTAC TCGGAAACTA TGTTCAAGAA GGCGAAAACA 
AGAGCTAAAA AGAAGCCACG GAAGCGCTCA GACAGTTCAG GAGGCTACAC CCTTTCAGAT 
GTTATCCAGA GTCCACCGTC TGCAGGATTA TTAAAGTCTG CAAAGACCAA TTCTGTGGAG 
TCTCTTCCAG AATTGTTGAC CTCAGATTCG GAAGGAAGCT ATGCAGGAGT GGCGAGTCCC 
AGAGACCTCC AGTCGCCCGA CTTCACAGCA GGATTCCATT CGGATAAGGT CGAGGGGAAA 
GCTAAGCCAT ACGTGAATGG TATACCTCCT CCATGTACTA GGGAAGATGT AAAGCCGTGG 
GAGAAATCAC CAACAACTAA GTCTGCTCCC CAGTTCATCC CCAGTAACAG AGTGGACACT 
GCAGCCTCTT CCAGTTGGCT TGCCGGCTCT TGCAGTCCTG TGAGTCCTCC TGTTGTGGAT 
CTTAGAACCA TCATGGAAAC AGAAGAAAAC AGACAGAAAT ATGGCGCAGC ACCAAAGTCA 
AACTTAGGAA AGATTATTTC TCACGGAATC AAACTTTCTC AGAAGCAACG GAAAATGATT 
GCATTGACCA CCAAAGAAAA CAATTCAGGA ACGAACAGCA TGGAAGCCAT TTTGACAGCT 
CCTTCCAAGT CCCCCAAGCC TGCGAATGCA TGGGCACCTC TGCACTCACC TTTATCCAGG 
TCATTCCGGG ATTTTTTACT AGAAGAAAAA AAACCCGTTC CTGGCTATGG TTCAGGAGAT 
CATGTCAAAA AAGTTTGTTT TAAAGGAACT GAAAACTCTC CAGCCCTAAA TGTTGCAAGA 
TGTTCAACCC ATGGAACTCC AGGATTAGAA AGCAATCATG TTTCTGATTT TCCACTTCTA 
GACAGTCCCA ACCCCTGGCA GTCTTCCTCC CTGGCGGCTT CTCCCGCAGT TGCTCCAGTC 
ACATTTGCAT CCATTGTAGA AGAAGAGCGG CAACAAGAAG CTGCCCTCAT CAGAAGCCGG 
GAGAAACCCT TGGCTCTCAT TCAGGTCGAG GAGCATGCAA TACAAGACTT GCTGGTTTTC 
TATGAGGCAT TTGGCAACCC CGAGGAGTTT GTCGTCGTTG AAAGGGCACC ACAGGGGCCT 
CTGGCAGTGC CTATGTGGAA CAAGCATGGC TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ibtk ( NM_001081282.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074751.1 Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02023
Clone ID Related Accession (Same CDS sequence)	NM_001081282.2
Accession Version	NM_001081282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	inhibitor of Bruton tyrosine kinase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB639555.1, BC137798.1, AK031796.1 and BP760552.1. On Dec 9, 2010 this sequence version replaced NM_001081282.1. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: BC137798.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCTG CCACCCCCGA CTGCACGTCA AAATGCAGAT CCCTGAAGCA TGCGCTGGAT 
GTCCTTTCTG TGGTGACAAA GGGGAGTGAG AGCCAGATCA AGTCCTTCCT CGCCAGGTAC 
TGCTACAATG CTGCAACTGT CAAGGATGCC TTTGGCAGGA ACGCCGGCCA CCTGGCTTCC 
TCCTGCGGGA AGAAAGGAGT GTTGGACTGG CTGATTGAGA AAGGAGTGGA CTTGCTGGTG 
AAAGACAAGG AGTCCGGCTG GACAGCACTG CACAGAAGCG TTTTTTATGG ACATATCGAT 
TGTGTTTGGT CTCTGTTGAA GCATGGTGTT AGCTTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTTAT GAAGGACAGG CCAACTCATG TTGTATTTAA GGACACTGAT 
CCCACAGAAG TCTATACTTG GGGAGATAAC ACAAACTTCA CTCTGGGCCA CGGAAGCCAG 
AATAGCAAAC ATCACCCCGA GCTGCTGGAC CTGTTCTCCA GGAGTGGGGT TTATGTCAAG 
CAGGTGGTGC TTTGCAAATT TCATTCGGTG TTTCTGTCCC AGAAAGGGCA GGTTTACACC 
TGTGGCCATG GTCGCGGAGG ACGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGGCTTGTGG AAGGACTGTC TGGTCACAAT TGTTCGCAAG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTGT TAACTGATGA TGGATGTGTC TATACATTTG GTCTCAACAT GTTTCACCAA 
TTAGGAATTA TTCCTCCACC TGCTAGTTGT AATGTACCCA GACAGATACA GGCAAAATAT 
CTGAAAGGAA GGACTATCAT TGGAGTAGCA GCGGGCAGGT TTCATACGGT GCTGTGGACT 
CGAGAAGCCG TTTACACACT GGGACTGAAT GGTGGACAGT TGGGTCACTT ACTGGATCCC 
AATGGTGAAA AGTGTGTCAC AACCCCTCGC CAAGTCTCTG CCCTTCATCA CAAGGATATC 
GCTGTGTCTT TGGTAGCTGC AAGTGATGGT GCCACAGTCT GTGTCACCAC AAGGGGAGAC 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCAA CTAAACAACT GAACTTGAAA 
AAGGTTCTTG TCTCTGGAGG TTGTATGGAA TACAAGGTTG ATCCTGAACA TTTGACAGAA 
AATGGAGGTC AGAAAATATG TGTCCTCGCT ATGGATGGAG CTGGAAGGGT GTTTTGCTGG 
AGATCAATCA GCAGTTCTCT GAAGCAGTGC CGATGGGCCT ATCCTCGCCA GGTGTCAATC 
TCCGATATTG CTTTAAATAG AAATGAAATT CTGTTTGTCA CACAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGA CAAAAGAAAG AATTCTGAAA AGAAAGCAGA CATTTTACCA 
AACCTCCATC ATTCCTCATC AGATGTGTCT TGTGTCCCTG ATACAAATAG TGTGTATGAA 
AGAATTCGAC TTGAGAAACT TCCTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGGTGCA ACTTTGCAAT CCTACAGTCA GACCCTAAAA CAAGCCTTTA TGAGATTCCA 
GTTGTGTCTT CATCATCTTT TTTTGAAGAA TTTGGCAAGC TCCTGAGGGA GACAGATGAA 
ATGGACAGCT TCCACGATGT GACCTTTCAA GTTGGCAATA GACACTTCCC CGCACATAAA 
TACATTTTGG CCGTGCGCTC TGACTTCTTC CAGAAGTTGT TTCTTTCAGA TGGCTCTTCT 
TTAGAGCTGA CGGATGTTTA CCAGAAAGAC GAAGATGCTG CTGGCTGCCA TCTCTTTGTG 
GTGGAGAAAG TCCATCCTGA CTTGTTCGAA TACCTCTTAC AGTTCATGTA CACAGATACC 
TGTGACTTGT TAACTCATGG CTTCAAACCA AGAATGATCG TGAAGAGAAA GGCAGAAGAC 
TGTGAGGGCT CCCCAGATTC TCATCTGCAC ACTGTGAATT GCCATGTGGA TGATAAACAG 
AAGTCAGCAT TTGAGGTGTA CAGAAGCAAC CAGGCCCACA CACTTAGTGA ACGGCAGAAG 
AGCAAACCCA AATCTTCTAA GAAGGGAAAA GGTGTTGGGG ACGACGACCC TGTAAGGATG 
TTGCAAAGTG TTGCTAAGAA GTTTGGCCTC AGTAATTTGA GTAGCAGGTT AGAAGGAGTC 
AGATTGGAAA ATGAAAAAAT TAATGTTATT GCGAAGAAAA CTGGCAATAA ACTGAAGCTA 
AGTCAGAAAA AATGTTCGTT TCTGTATGAT GTCACCATGA AGTCTGTGGA TGGAAAGGAG 
TTCTCCTGCC ATAAGTGTGT GCTCTGTGCG AGACTTGAGT ATTTCCACAG CATGCTTAGC 
AGATCGTGGA TTGAGGCCTC CAGCTGTGCA GCTCTGGAAA TGCCAATCCA GTCTGAGATT 
CTGAAAGTGA TTTTGGACTA CCTCTACACT GATGAGGCTG TGGTGATAAA AGAGTCTCAA 
AACGTGGACT TTGTTTGTAG TGTTCTTGTC GTGGCTGATC AGCTTCTCAT AACTCGGTTA 
AAGGAGATTT GTGAAGTAGC ATTGACTGAA AACCTTACCC TTAAGAATGC TGCGATGCTG 
CTGGAGTTTG CAGCCTTGTA CAATGCTGGG CAGTTGAAGC TCTCTTGTTT ACAGTTCATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTGG ATGTTTTAAG TGAGGATGTT 
TTGAAGGACC TTTCCATATT CTACAGAAAA ATGATTCCAG CAATGGAGAG AAGAGTCATT 
ACACCGTATC AAGATGGGCC AGATATTAGC TCTATGCAAG TAGAAGACGG AGAGGTCTTT 
TTTAAAGAAG AAATAAACAT GGAACCAAAT TACTCGGAAA CTATGTTCAA GAAGGCGAAA 
ACAAGAGCTA AAAAGAAGCC ACGGAAGCGC TCAGACAGTT CAGGAGGCTA CACCCTTTCA 
GATGTTATCC AGAGTCCACC GTCTGCAGGA TTATTAAAGT CTGCAAAGAC CAATTCTGTG 
GAGTCTCTTC CAGAATTGTT GACCTCAGAT TCGGAAGGAA GCTATGCAGG AGTGGCGAGT 
CCCAGAGACC TCCAGTCGCC CGACTTCACA GCAGGATTCC ATTCGGATAA GGTCGAGGGG 
AAAGCTAAGC CATACGTGAA TGGTATACCT CCTCCATGTA CTAGGGAAGA TGTAAAGCCG 
TGGGAGAAAT CACCAACAAC TAAGTCTGCT CCCCAGTTCA TCCCCAGTAA CAGAGTGGAC 
ACTGCAGCCT CTTCCAGTTG GCTTGCCGGC TCTTGCAGTC CTGTGAGTCC TCCTGTTGTG 
GATCTTAGAA CCATCATGGA AACAGAAGAA AACAGACAGA AATATGGCGC AGCACCAAAG 
TCAAACTTAG GAAAGATTAT TTCTCACGGA ATCAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCATTGA CCACCAAAGA AAACAATTCA GGAACGAACA GCATGGAAGC CATTTTGACA 
GCTCCTTCCA AGTCCCCCAA GCCTGCGAAT GCATGGGCAC CTCTGCACTC ACCTTTATCC 
AGGTCATTCC GGGATTTTTT ACTAGAAGAA AAAAAACCCG TTCCTGGCTA TGGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ACTGAAAACT CTCCAGCCCT AAATGTTGCA 
AGATGTTCAA CCCATGGAAC TCCAGGATTA GAAAGCAATC ATGTTTCTGA TTTTCCACTT 
CTAGACAGTC CCAACCCCTG GCAGTCTTCC TCCCTGGCGG CTTCTCCCGC AGTTGCTCCA 
GTCACATTTG CATCCATTGT AGAAGAAGAG CGGCAACAAG AAGCTGCCCT CATCAGAAGC 
CGGGAGAAAC CCTTGGCTCT CATTCAGGTC GAGGAGCATG CAATACAAGA CTTGCTGGTT 
TTCTATGAGG CATTTGGCAA CCCCGAGGAG TTTGTCGTCG TTGAAAGGGC ACCACAGGGG 
CCTCTGGCAG TGCCTATGTG GAACAAGCAT GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074751.1
CDS	569..4627
Translation	MDAATPDCTSKCRSLKHALDVLSVVTKGSESQIKSFLARYCYNAATVKDAFGRNAGHLASSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSVFYGHIDCVWSLLKHGVSLYMQDKEGLSPLDLLMKDRPTHVVFKDTDPTEVYTWGDNTNFTLGHGSQNSKHHPELLDLFSRSGVYVKQVVLCKFHSVFLSQKGQVYTCGHGRGGRLGHGDEQTCLVPRLVEGLSGHNCSQVAAAKDHTVVLTDDGCVYTFGLNMFHQLGIIPPPASCNVPRQIQAKYLKGRTIIGVAAGRFHTVLWTREAVYTLGLNGGQLGHLLDPNGEKCVTTPRQVSALHHKDIAVSLVAASDGATVCVTTRGDIYLLADYQCKKMATKQLNLKKVLVSGGCMEYKVDPEHLTENGGQKICVLAMDGAGRVFCWRSISSSLKQCRWAYPRQVSISDIALNRNEILFVTQDGEGFKGKWFEDKRKNSEKKADILPNLHHSSSDVSCVPDTNSVYERIRLEKLPFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPVVSSSSFFEEFGKLLRETDEMDSFHDVTFQVGNRHFPAHKYILAVRSDFFQKLFLSDGSSLELTDVYQKDEDAAGCHLFVVEKVHPDLFEYLLQFMYTDTCDLLTHGFKPRMIVKRKAEDCEGSPDSHLHTVNCHVDDKQKSAFEVYRSNQAHTLSERQKSKPKSSKKGKGVGDDDPVRMLQSVAKKFGLSNLSSRLEGVRLENEKINVIAKKTGNKLKLSQKKCSFLYDVTMKSVDGKEFSCHKCVLCARLEYFHSMLSRSWIEASSCAALEMPIQSEILKVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKEICEVALTENLTLKNAAMLLEFAALYNAGQLKLSCLQFIGLNMAALLEARSLDVLSEDVLKDLSIFYRKMIPAMERRVITPYQDGPDISSMQVEDGEVFFKEEINMEPNYSETMFKKAKTRAKKKPRKRSDSSGGYTLSDVIQSPPSAGLLKSAKTNSVESLPELLTSDSEGSYAGVASPRDLQSPDFTAGFHSDKVEGKAKPYVNGIPPPCTREDVKPWEKSPTTKSAPQFIPSNRVDTAASSSWLAGSCSPVSPPVVDLRTIMETEENRQKYGAAPKSNLGKIISHGIKLSQKQRKMIALTTKENNSGTNSMEAILTAPSKSPKPANAWAPLHSPLSRSFRDFLLEEKKPVPGYGSGDHVKKVCFKGTENSPALNVARCSTHGTPGLESNHVSDFPLLDSPNPWQSSSLAASPAVAPVTFASIVEEERQQEAALIRSREKPLALIQVEEHAIQDLLVFYEAFGNPEEFVVVERAPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	NM_001081282.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081282.2

ORF Insert Sequence:

ATGGATGCTG CCACCCCCGA CTGCACGTCA AAATGCAGAT CCCTGAAGCA TGCGCTGGAT 
GTCCTTTCTG TGGTGACAAA GGGGAGTGAG AGCCAGATCA AGTCCTTCCT CGCCAGGTAC 
TGCTACAATG CTGCAACTGT CAAGGATGCC TTTGGCAGGA ACGCCGGCCA CCTGGCTTCC 
TCCTGCGGGA AGAAAGGAGT GTTGGACTGG CTGATTGAGA AAGGAGTGGA CTTGCTGGTG 
AAAGACAAGG AGTCCGGCTG GACAGCACTG CACAGAAGCG TTTTTTATGG ACATATCGAT 
TGTGTTTGGT CTCTGTTGAA GCATGGTGTT AGCTTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTTAT GAAGGACAGG CCAACTCATG TTGTATTTAA GGACACTGAT 
CCCACAGAAG TCTATACTTG GGGAGATAAC ACAAACTTCA CTCTGGGCCA CGGAAGCCAG 
AATAGCAAAC ATCACCCCGA GCTGCTGGAC CTGTTCTCCA GGAGTGGGGT TTATGTCAAG 
CAGGTGGTGC TTTGCAAATT TCATTCGGTG TTTCTGTCCC AGAAAGGGCA GGTTTACACC 
TGTGGCCATG GTCGCGGAGG ACGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGGCTTGTGG AAGGACTGTC TGGTCACAAT TGTTCGCAAG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTGT TAACTGATGA TGGATGTGTC TATACATTTG GTCTCAACAT GTTTCACCAA 
TTAGGAATTA TTCCTCCACC TGCTAGTTGT AATGTACCCA GACAGATACA GGCAAAATAT 
CTGAAAGGAA GGACTATCAT TGGAGTAGCA GCGGGCAGGT TTCATACGGT GCTGTGGACT 
CGAGAAGCCG TTTACACACT GGGACTGAAT GGTGGACAGT TGGGTCACTT ACTGGATCCC 
AATGGTGAAA AGTGTGTCAC AACCCCTCGC CAAGTCTCTG CCCTTCATCA CAAGGATATC 
GCTGTGTCTT TGGTAGCTGC AAGTGATGGT GCCACAGTCT GTGTCACCAC AAGGGGAGAC 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCAA CTAAACAACT GAACTTGAAA 
AAGGTTCTTG TCTCTGGAGG TTGTATGGAA TACAAGGTTG ATCCTGAACA TTTGACAGAA 
AATGGAGGTC AGAAAATATG TGTCCTCGCT ATGGATGGAG CTGGAAGGGT GTTTTGCTGG 
AGATCAATCA GCAGTTCTCT GAAGCAGTGC CGATGGGCCT ATCCTCGCCA GGTGTCAATC 
TCCGATATTG CTTTAAATAG AAATGAAATT CTGTTTGTCA CACAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGA CAAAAGAAAG AATTCTGAAA AGAAAGCAGA CATTTTACCA 
AACCTCCATC ATTCCTCATC AGATGTGTCT TGTGTCCCTG ATACAAATAG TGTGTATGAA 
AGAATTCGAC TTGAGAAACT TCCTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGGTGCA ACTTTGCAAT CCTACAGTCA GACCCTAAAA CAAGCCTTTA TGAGATTCCA 
GTTGTGTCTT CATCATCTTT TTTTGAAGAA TTTGGCAAGC TCCTGAGGGA GACAGATGAA 
ATGGACAGCT TCCACGATGT GACCTTTCAA GTTGGCAATA GACACTTCCC CGCACATAAA 
TACATTTTGG CCGTGCGCTC TGACTTCTTC CAGAAGTTGT TTCTTTCAGA TGGCTCTTCT 
TTAGAGCTGA CGGATGTTTA CCAGAAAGAC GAAGATGCTG CTGGCTGCCA TCTCTTTGTG 
GTGGAGAAAG TCCATCCTGA CTTGTTCGAA TACCTCTTAC AGTTCATGTA CACAGATACC 
TGTGACTTGT TAACTCATGG CTTCAAACCA AGAATGATCG TGAAGAGAAA GGCAGAAGAC 
TGTGAGGGCT CCCCAGATTC TCATCTGCAC ACTGTGAATT GCCATGTGGA TGATAAACAG 
AAGTCAGCAT TTGAGGTGTA CAGAAGCAAC CAGGCCCACA CACTTAGTGA ACGGCAGAAG 
AGCAAACCCA AATCTTCTAA GAAGGGAAAA GGTGTTGGGG ACGACGACCC TGTAAGGATG 
TTGCAAAGTG TTGCTAAGAA GTTTGGCCTC AGTAATTTGA GTAGCAGGTT AGAAGGAGTC 
AGATTGGAAA ATGAAAAAAT TAATGTTATT GCGAAGAAAA CTGGCAATAA ACTGAAGCTA 
AGTCAGAAAA AATGTTCGTT TCTGTATGAT GTCACCATGA AGTCTGTGGA TGGAAAGGAG 
TTCTCCTGCC ATAAGTGTGT GCTCTGTGCG AGACTTGAGT ATTTCCACAG CATGCTTAGC 
AGATCGTGGA TTGAGGCCTC CAGCTGTGCA GCTCTGGAAA TGCCAATCCA GTCTGAGATT 
CTGAAAGTGA TTTTGGACTA CCTCTACACT GATGAGGCTG TGGTGATAAA AGAGTCTCAA 
AACGTGGACT TTGTTTGTAG TGTTCTTGTC GTGGCTGATC AGCTTCTCAT AACTCGGTTA 
AAGGAGATTT GTGAAGTAGC ATTGACTGAA AACCTTACCC TTAAGAATGC TGCGATGCTG 
CTGGAGTTTG CAGCCTTGTA CAATGCTGGG CAGTTGAAGC TCTCTTGTTT ACAGTTCATA 
GGACTGAATA TGGCAGCTTT ACTTGAAGCA AGGTCTCTGG ATGTTTTAAG TGAGGATGTT 
TTGAAGGACC TTTCCATATT CTACAGAAAA ATGATTCCAG CAATGGAGAG AAGAGTCATT 
ACACCGTATC AAGATGGGCC AGATATTAGC TCTATGCAAG TAGAAGACGG AGAGGTCTTT 
TTTAAAGAAG AAATAAACAT GGAACCAAAT TACTCGGAAA CTATGTTCAA GAAGGCGAAA 
ACAAGAGCTA AAAAGAAGCC ACGGAAGCGC TCAGACAGTT CAGGAGGCTA CACCCTTTCA 
GATGTTATCC AGAGTCCACC GTCTGCAGGA TTATTAAAGT CTGCAAAGAC CAATTCTGTG 
GAGTCTCTTC CAGAATTGTT GACCTCAGAT TCGGAAGGAA GCTATGCAGG AGTGGCGAGT 
CCCAGAGACC TCCAGTCGCC CGACTTCACA GCAGGATTCC ATTCGGATAA GGTCGAGGGG 
AAAGCTAAGC CATACGTGAA TGGTATACCT CCTCCATGTA CTAGGGAAGA TGTAAAGCCG 
TGGGAGAAAT CACCAACAAC TAAGTCTGCT CCCCAGTTCA TCCCCAGTAA CAGAGTGGAC 
ACTGCAGCCT CTTCCAGTTG GCTTGCCGGC TCTTGCAGTC CTGTGAGTCC TCCTGTTGTG 
GATCTTAGAA CCATCATGGA AACAGAAGAA AACAGACAGA AATATGGCGC AGCACCAAAG 
TCAAACTTAG GAAAGATTAT TTCTCACGGA ATCAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCATTGA CCACCAAAGA AAACAATTCA GGAACGAACA GCATGGAAGC CATTTTGACA 
GCTCCTTCCA AGTCCCCCAA GCCTGCGAAT GCATGGGCAC CTCTGCACTC ACCTTTATCC 
AGGTCATTCC GGGATTTTTT ACTAGAAGAA AAAAAACCCG TTCCTGGCTA TGGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ACTGAAAACT CTCCAGCCCT AAATGTTGCA 
AGATGTTCAA CCCATGGAAC TCCAGGATTA GAAAGCAATC ATGTTTCTGA TTTTCCACTT 
CTAGACAGTC CCAACCCCTG GCAGTCTTCC TCCCTGGCGG CTTCTCCCGC AGTTGCTCCA 
GTCACATTTG CATCCATTGT AGAAGAAGAG CGGCAACAAG AAGCTGCCCT CATCAGAAGC 
CGGGAGAAAC CCTTGGCTCT CATTCAGGTC GAGGAGCATG CAATACAAGA CTTGCTGGTT 
TTCTATGAGG CATTTGGCAA CCCCGAGGAG TTTGTCGTCG TTGAAAGGGC ACCACAGGGG 
CCTCTGGCAG TGCCTATGTG GAACAAGCAT GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ibtk ( XM_006510765.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510828.1 Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant X1, mRNA.

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CloneID	OMu42510
Clone ID Related Accession (Same CDS sequence)	XM_006510765.3 , XM_006510765.4
Accession Version	XM_006510765.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG CCACCCCCGA CTGCACGTCA AAATGCAGAT CCCTGAAGCA TGCGCTGGAT 
GTCCTTTCTG TGGTGACAAA GGGGAGTGAG AGCCAGATCA AGTCCTTCCT CGCCAGGTAC 
TGCTACAATG CTGCAACTGT CAAGGATGCC TTTGGCAGGA ACGCCGGCCA CCTGGCTTCC 
TCCTGCGGGA AGAAAGGAGT GTTGGACTGG CTGATTGAGA AAGGAGTGGA CTTGCTGGTG 
AAAGACAAGG AGTCCGGCTG GACAGCACTG CACAGAAGCG TTTTTTATGG ACATATCGAT 
TGTGTTTGGT CTCTGTTGAA GCATGGTGTT AGCTTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTTAT GAAGGACAGG CCAACTCATG TTGTATTTAA GGACACTGAT 
CCCACAGAAG TCTATACTTG GGGAGATAAC ACAAACTTCA CTCTGGGCCA CGGAAGCCAG 
AATAGCAAAC ATCACCCCGA GCTGCTGGAC CTGTTCTCCA GGAGTGGGGT TTATGTCAAG 
CAGGTGGTGC TTTGCAAATT TCATTCGGTG TTTCTGTCCC AGAAAGGGCA GGTTTACACC 
TGTGGCCATG GTCGCGGAGG ACGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGGCTTGTGG AAGGACTGTC TGGTCACAAT TGTTCGCAAG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTGT TAACTGATGA TGGATGTGTC TATACATTTG GTCTCAACAT GTTTCACCAA 
TTAGGAATTA TTCCTCCACC TGCTAGTTGT AATGTACCCA GACAGATACA GGCAAAATAT 
CTGAAAGGAA GGACTATCAT TGGAGTAGCA GCGGGCAGGT TTCATACGGT GCTGTGGACT 
CGAGAAGCCG TTTACACACT GGGACTGAAT GGTGGACAGT TGGGTCACTT ACTGGATCCC 
AATGGTGAAA AGTGTGTCAC AACCCCTCGC CAAGTCTCTG CCCTTCATCA CAAGGATATC 
GCTGTGTCTT TGGTAGCTGC AAGTGATGGT GCCACAGTCT GTGTCACCAC AAGGGGAGAC 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCAA CTAAACAACT GAACTTGAAA 
AAGGTTCTTG TCTCTGGAGG TTGTATGGAA TACAAGGTTG ATCCTGAACA TTTGACAGAA 
AATGGAGGTC AGAAAATATG TGTCCTCGCT ATGGATGGAG CTGGAAGGGT GTTTTGCTGG 
AGATCAATCA GCAGTTCTCT GAAGCAGTGC CGATGGGCCT ATCCTCGCCA GGTGTCAATC 
TCCGATATTG CTTTAAATAG AAATGAAATT CTGTTTGTCA CACAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGA CAAAAGAAAG AATTCTGAAA AGAAAGACAT TTTACCAAAC 
CTCCATCATT CCTCATCAGA TGTGTCTTGT GTCCCTGATA CAAATAGTGT GTATGAAAGA 
ATTCGACTTG AGAAACTTCC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGGTGCAACT TTGCAATCCT ACAGTCAGAC CCTAAAACAA GCCTTTATGA GATTCCAGTT 
GTGTCTTCAT CATCTTTTTT TGAAGAATTT GGCAAGCTCC TGAGGGAGAC AGATGAAATG 
GACAGCTTCC ACGATGTGAC CTTTCAAGTT GGCAATAGAC ACTTCCCCGC ACATAAATAC 
ATTTTGGCCG TGCGCTCTGA CTTCTTCCAG AAGTTGTTTC TTTCAGATGG CTCTTCTTTA 
GAGCTGACGG ATGTTTACCA GAAAGACGAA GATGCTGCTG GCTGCCATCT CTTTGTGGTG 
GAGAAAGTCC ATCCTGACTT GTTCGAATAC CTCTTACAGT TCATGTACAC AGATACCTGT 
GACTTGTTAA CTCATGGCTT CAAACCAAGA ATGATCGTGA AGAGAAAGGC AGAAGACTGT 
GAGGGCTCCC CAGATTCTCA TCTGCACACT GTGAATTGCC ATGTGGATGA TAAACAGAAG 
TCAGCATTTG AGGTGTACAG AAGCAACCAG GCCCACACAC TTAGTGAACG GCAGAAGAGC 
AAACCCAAAT CTTCTAAGAA GGGAAAAGGT GTTGGGGACG ACGACCCTGT AAGGATGTTG 
CAAAGTGTTG CTAAGAAGTT TGGCCTCAGT AATTTGAGTA GCAGGTTAGA AGGAGTCAGA 
TTGGAAAATG AAAAAATTAA TGTTATTGCG AAGAAAACTG GCAATAAACT GAAGCTAAGT 
CAGAAAAAAT GTTCGTTTCT GTATGATGTC ACCATGAAGT CTGTGGATGG AAAGGAGTTC 
TCCTGCCATA AGTGTGTGCT CTGTGCGAGA CTTGAGTATT TCCACAGCAT GCTTAGCAGA 
TCGTGGATTG AGGCCTCCAG CTGTGCAGCT CTGGAAATGC CAATCCAGTC TGAGATTCTG 
AAAGTGATTT TGGACTACCT CTACACTGAT GAGGCTGTGG TGATAAAAGA GTCTCAAAAC 
GTGGACTTTG TTTGTAGTGT TCTTGTCGTG GCTGATCAGC TTCTCATAAC TCGGTTAAAG 
GAGATTTGTG AAGTAGCATT GACTGAAAAC CTTACCCTTA AGAATGCTGC GATGCTGCTG 
GAGTTTGCAG CCTTGTACAA TGCTGGGCAG TTGAAGCTCT CTTGTTTACA GTTCATAGGA 
CTGAATATGG CAGCTTTACT TGAAGCAAGG TCTCTGGATG TTTTAAGTGA GGATGTTTTG 
AAGGACCTTT CCATATTCTA CAGAAAAATG ATTCCAGCAA TGGAGAGAAG AGTCATTACA 
CCGTATCAAG ATGGGCCAGA TATTAGCTCT ATGCAAGTAG AAGACGGAGA GGTCTTTTTT 
AAAGAAGAAA TAAACATGGA ACCAAATTAC TCGGAAACTA TGTTCAAGAA GGCGAAAACA 
AGAGCTAAAA AGAAGCCACG GAAGCGCTCA GACAGTTCAG GAGGCTACAC CCTTTCAGAT 
GTTATCCAGA GTCCACCGTC TGCAGGATTA TTAAAGTCTG CAAAGACCAA TTCTGTGGAG 
TCTCTTCCAG AATTGTTGAC CTCAGATTCG GAAGGAAGCT ATGCAGGAGT GGCGAGTCCC 
AGAGACCTCC AGTCGCCCGA CTTCACAGCA GGATTCCATT CGGATAAGGT CGAGGGGAAA 
GCTAAGCCAT ACGTGAATGG TATACCTCCT CCATGTACTA GGGAAGATGT AAAGCCGTGG 
GAGAAATCAC CAACAACTAA GTCTGCTCCC CAGTTCATCC CCAGTAACAG AGTGGACACT 
GCAGCCTCTT CCAGTTGGCT TGCCGGCTCT TGCAGTCCTG TGAGTCCTCC TGTTGTGGAT 
CTTAGAACCA TCATGGAAAC AGAAGAAAAC AGACAGAAAT ATGGCGCAGC ACCAAAGTCA 
AACTTAGGAA AGATTATTTC TCACGGAATC AAACTTTCTC AGAAGCAACG GAAAATGATT 
GCATTGACCA CCAAAGAAAA CAATTCAGGA ACGAACAGCA TGGAAGCCAT TTTGACAGCT 
CCTTCCAAGT CCCCCAAGCC TGCGAATGCA TGGGCACCTC TGCACTCACC TTTATCCAGG 
TCATTCCGGG ATTTTTTACT AGAAGAAAAA AAACCCGTTC CTGGCTATGG TTCAGGAGAT 
CATGTCAAAA AAGTTTGTTT TAAAGGAACT GAAAACTCTC CAGCCCTAAA TGTTGCAAGA 
TGTTCAACCC ATGGAACTCC AGGATTAGAA AGCAATCATG TTTCTGATTT TCCACTTCTA 
GACAGTCCCA ACCCCTGGCA GTCTTCCTCC CTGGCGGCTT CTCCCGCAGT TGCTCCAGTC 
ACATTTGCAT CCATTGTAGA AGAAGAGCGG CAACAAGAAG CTGCCCTCAT CAGAAGCCGG 
GAGAAACCCT TGGCTCTCAT TCAGGTCGAG GAGCATGCAA TACAAGACTT GCTGGTTTTC 
TATGAGGCAT TTGGCAACCC CGAGGAGTTT GTCGTCGTTG AAAGGGCACC ACAGGGGCCT 
CTGGCAGTGC CTATGTGGAA CAAGCATGGC TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510828.1
CDS	1670..5725
Translation	MDAATPDCTSKCRSLKHALDVLSVVTKGSESQIKSFLARYCYNAATVKDAFGRNAGHLASSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSVFYGHIDCVWSLLKHGVSLYMQDKEGLSPLDLLMKDRPTHVVFKDTDPTEVYTWGDNTNFTLGHGSQNSKHHPELLDLFSRSGVYVKQVVLCKFHSVFLSQKGQVYTCGHGRGGRLGHGDEQTCLVPRLVEGLSGHNCSQVAAAKDHTVVLTDDGCVYTFGLNMFHQLGIIPPPASCNVPRQIQAKYLKGRTIIGVAAGRFHTVLWTREAVYTLGLNGGQLGHLLDPNGEKCVTTPRQVSALHHKDIAVSLVAASDGATVCVTTRGDIYLLADYQCKKMATKQLNLKKVLVSGGCMEYKVDPEHLTENGGQKICVLAMDGAGRVFCWRSISSSLKQCRWAYPRQVSISDIALNRNEILFVTQDGEGFKGKWFEDKRKNSEKKDILPNLHHSSSDVSCVPDTNSVYERIRLEKLPFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPVVSSSSFFEEFGKLLRETDEMDSFHDVTFQVGNRHFPAHKYILAVRSDFFQKLFLSDGSSLELTDVYQKDEDAAGCHLFVVEKVHPDLFEYLLQFMYTDTCDLLTHGFKPRMIVKRKAEDCEGSPDSHLHTVNCHVDDKQKSAFEVYRSNQAHTLSERQKSKPKSSKKGKGVGDDDPVRMLQSVAKKFGLSNLSSRLEGVRLENEKINVIAKKTGNKLKLSQKKCSFLYDVTMKSVDGKEFSCHKCVLCARLEYFHSMLSRSWIEASSCAALEMPIQSEILKVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKEICEVALTENLTLKNAAMLLEFAALYNAGQLKLSCLQFIGLNMAALLEARSLDVLSEDVLKDLSIFYRKMIPAMERRVITPYQDGPDISSMQVEDGEVFFKEEINMEPNYSETMFKKAKTRAKKKPRKRSDSSGGYTLSDVIQSPPSAGLLKSAKTNSVESLPELLTSDSEGSYAGVASPRDLQSPDFTAGFHSDKVEGKAKPYVNGIPPPCTREDVKPWEKSPTTKSAPQFIPSNRVDTAASSSWLAGSCSPVSPPVVDLRTIMETEENRQKYGAAPKSNLGKIISHGIKLSQKQRKMIALTTKENNSGTNSMEAILTAPSKSPKPANAWAPLHSPLSRSFRDFLLEEKKPVPGYGSGDHVKKVCFKGTENSPALNVARCSTHGTPGLESNHVSDFPLLDSPNPWQSSSLAASPAVAPVTFASIVEEERQQEAALIRSREKPLALIQVEEHAIQDLLVFYEAFGNPEEFVVVERAPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_006510765.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus inhibitor of Bruton agammaglobulinemia tyrosine kinase (Ibtk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510765.3

ORF Insert Sequence:

ATGGATGCTG CCACCCCCGA CTGCACGTCA AAATGCAGAT CCCTGAAGCA TGCGCTGGAT 
GTCCTTTCTG TGGTGACAAA GGGGAGTGAG AGCCAGATCA AGTCCTTCCT CGCCAGGTAC 
TGCTACAATG CTGCAACTGT CAAGGATGCC TTTGGCAGGA ACGCCGGCCA CCTGGCTTCC 
TCCTGCGGGA AGAAAGGAGT GTTGGACTGG CTGATTGAGA AAGGAGTGGA CTTGCTGGTG 
AAAGACAAGG AGTCCGGCTG GACAGCACTG CACAGAAGCG TTTTTTATGG ACATATCGAT 
TGTGTTTGGT CTCTGTTGAA GCATGGTGTT AGCTTGTATA TGCAAGATAA AGAAGGATTG 
TCACCTTTGG ATCTTCTTAT GAAGGACAGG CCAACTCATG TTGTATTTAA GGACACTGAT 
CCCACAGAAG TCTATACTTG GGGAGATAAC ACAAACTTCA CTCTGGGCCA CGGAAGCCAG 
AATAGCAAAC ATCACCCCGA GCTGCTGGAC CTGTTCTCCA GGAGTGGGGT TTATGTCAAG 
CAGGTGGTGC TTTGCAAATT TCATTCGGTG TTTCTGTCCC AGAAAGGGCA GGTTTACACC 
TGTGGCCATG GTCGCGGAGG ACGGTTAGGC CATGGAGATG AACAGACATG CTTGGTTCCC 
AGGCTTGTGG AAGGACTGTC TGGTCACAAT TGTTCGCAAG TGGCAGCTGC TAAGGATCAC 
ACTGTTGTGT TAACTGATGA TGGATGTGTC TATACATTTG GTCTCAACAT GTTTCACCAA 
TTAGGAATTA TTCCTCCACC TGCTAGTTGT AATGTACCCA GACAGATACA GGCAAAATAT 
CTGAAAGGAA GGACTATCAT TGGAGTAGCA GCGGGCAGGT TTCATACGGT GCTGTGGACT 
CGAGAAGCCG TTTACACACT GGGACTGAAT GGTGGACAGT TGGGTCACTT ACTGGATCCC 
AATGGTGAAA AGTGTGTCAC AACCCCTCGC CAAGTCTCTG CCCTTCATCA CAAGGATATC 
GCTGTGTCTT TGGTAGCTGC AAGTGATGGT GCCACAGTCT GTGTCACCAC AAGGGGAGAC 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCAA CTAAACAACT GAACTTGAAA 
AAGGTTCTTG TCTCTGGAGG TTGTATGGAA TACAAGGTTG ATCCTGAACA TTTGACAGAA 
AATGGAGGTC AGAAAATATG TGTCCTCGCT ATGGATGGAG CTGGAAGGGT GTTTTGCTGG 
AGATCAATCA GCAGTTCTCT GAAGCAGTGC CGATGGGCCT ATCCTCGCCA GGTGTCAATC 
TCCGATATTG CTTTAAATAG AAATGAAATT CTGTTTGTCA CACAGGATGG GGAAGGATTT 
AAAGGGAAAT GGTTTGAAGA CAAAAGAAAG AATTCTGAAA AGAAAGACAT TTTACCAAAC 
CTCCATCATT CCTCATCAGA TGTGTCTTGT GTCCCTGATA CAAATAGTGT GTATGAAAGA 
ATTCGACTTG AGAAACTTCC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGGTGCAACT TTGCAATCCT ACAGTCAGAC CCTAAAACAA GCCTTTATGA GATTCCAGTT 
GTGTCTTCAT CATCTTTTTT TGAAGAATTT GGCAAGCTCC TGAGGGAGAC AGATGAAATG 
GACAGCTTCC ACGATGTGAC CTTTCAAGTT GGCAATAGAC ACTTCCCCGC ACATAAATAC 
ATTTTGGCCG TGCGCTCTGA CTTCTTCCAG AAGTTGTTTC TTTCAGATGG CTCTTCTTTA 
GAGCTGACGG ATGTTTACCA GAAAGACGAA GATGCTGCTG GCTGCCATCT CTTTGTGGTG 
GAGAAAGTCC ATCCTGACTT GTTCGAATAC CTCTTACAGT TCATGTACAC AGATACCTGT 
GACTTGTTAA CTCATGGCTT CAAACCAAGA ATGATCGTGA AGAGAAAGGC AGAAGACTGT 
GAGGGCTCCC CAGATTCTCA TCTGCACACT GTGAATTGCC ATGTGGATGA TAAACAGAAG 
TCAGCATTTG AGGTGTACAG AAGCAACCAG GCCCACACAC TTAGTGAACG GCAGAAGAGC 
AAACCCAAAT CTTCTAAGAA GGGAAAAGGT GTTGGGGACG ACGACCCTGT AAGGATGTTG 
CAAAGTGTTG CTAAGAAGTT TGGCCTCAGT AATTTGAGTA GCAGGTTAGA AGGAGTCAGA 
TTGGAAAATG AAAAAATTAA TGTTATTGCG AAGAAAACTG GCAATAAACT GAAGCTAAGT 
CAGAAAAAAT GTTCGTTTCT GTATGATGTC ACCATGAAGT CTGTGGATGG AAAGGAGTTC 
TCCTGCCATA AGTGTGTGCT CTGTGCGAGA CTTGAGTATT TCCACAGCAT GCTTAGCAGA 
TCGTGGATTG AGGCCTCCAG CTGTGCAGCT CTGGAAATGC CAATCCAGTC TGAGATTCTG 
AAAGTGATTT TGGACTACCT CTACACTGAT GAGGCTGTGG TGATAAAAGA GTCTCAAAAC 
GTGGACTTTG TTTGTAGTGT TCTTGTCGTG GCTGATCAGC TTCTCATAAC TCGGTTAAAG 
GAGATTTGTG AAGTAGCATT GACTGAAAAC CTTACCCTTA AGAATGCTGC GATGCTGCTG 
GAGTTTGCAG CCTTGTACAA TGCTGGGCAG TTGAAGCTCT CTTGTTTACA GTTCATAGGA 
CTGAATATGG CAGCTTTACT TGAAGCAAGG TCTCTGGATG TTTTAAGTGA GGATGTTTTG 
AAGGACCTTT CCATATTCTA CAGAAAAATG ATTCCAGCAA TGGAGAGAAG AGTCATTACA 
CCGTATCAAG ATGGGCCAGA TATTAGCTCT ATGCAAGTAG AAGACGGAGA GGTCTTTTTT 
AAAGAAGAAA TAAACATGGA ACCAAATTAC TCGGAAACTA TGTTCAAGAA GGCGAAAACA 
AGAGCTAAAA AGAAGCCACG GAAGCGCTCA GACAGTTCAG GAGGCTACAC CCTTTCAGAT 
GTTATCCAGA GTCCACCGTC TGCAGGATTA TTAAAGTCTG CAAAGACCAA TTCTGTGGAG 
TCTCTTCCAG AATTGTTGAC CTCAGATTCG GAAGGAAGCT ATGCAGGAGT GGCGAGTCCC 
AGAGACCTCC AGTCGCCCGA CTTCACAGCA GGATTCCATT CGGATAAGGT CGAGGGGAAA 
GCTAAGCCAT ACGTGAATGG TATACCTCCT CCATGTACTA GGGAAGATGT AAAGCCGTGG 
GAGAAATCAC CAACAACTAA GTCTGCTCCC CAGTTCATCC CCAGTAACAG AGTGGACACT 
GCAGCCTCTT CCAGTTGGCT TGCCGGCTCT TGCAGTCCTG TGAGTCCTCC TGTTGTGGAT 
CTTAGAACCA TCATGGAAAC AGAAGAAAAC AGACAGAAAT ATGGCGCAGC ACCAAAGTCA 
AACTTAGGAA AGATTATTTC TCACGGAATC AAACTTTCTC AGAAGCAACG GAAAATGATT 
GCATTGACCA CCAAAGAAAA CAATTCAGGA ACGAACAGCA TGGAAGCCAT TTTGACAGCT 
CCTTCCAAGT CCCCCAAGCC TGCGAATGCA TGGGCACCTC TGCACTCACC TTTATCCAGG 
TCATTCCGGG ATTTTTTACT AGAAGAAAAA AAACCCGTTC CTGGCTATGG TTCAGGAGAT 
CATGTCAAAA AAGTTTGTTT TAAAGGAACT GAAAACTCTC CAGCCCTAAA TGTTGCAAGA 
TGTTCAACCC ATGGAACTCC AGGATTAGAA AGCAATCATG TTTCTGATTT TCCACTTCTA 
GACAGTCCCA ACCCCTGGCA GTCTTCCTCC CTGGCGGCTT CTCCCGCAGT TGCTCCAGTC 
ACATTTGCAT CCATTGTAGA AGAAGAGCGG CAACAAGAAG CTGCCCTCAT CAGAAGCCGG 
GAGAAACCCT TGGCTCTCAT TCAGGTCGAG GAGCATGCAA TACAAGACTT GCTGGTTTTC 
TATGAGGCAT TTGGCAACCC CGAGGAGTTT GTCGTCGTTG AAAGGGCACC ACAGGGGCCT 
CTGGCAGTGC CTATGTGGAA CAAGCATGGC TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Btk regulation in human and mouse B cells via protein kinase C phosphorylation of IBtk?.
Blood117(24)6520-31(2011 Jun)
Janda E,Palmieri C,Pisano A,Pontoriero M,Iaccino E,Falcone C,Fiume G,Gaspari M,Nevolo M,Di Salle E,Rossi A,De Laurentiis A,Greco A,Di Napoli D,Verheij E,Britti D,Lavecchia L,Quinto I,Scala G

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Developmental cell18(4)675-85(2010 Apr)
Guo G,Huss M,Tong GQ,Wang C,Li Sun L,Clarke ND,Robson P

High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization.
Gene expression patterns : GEP6(2)213-24(2006 Jan)
Yoshikawa T,Piao Y,Zhong J,Matoba R,Carter MG,Wang Y,Goldberg I,Ko MS

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)
Nishimura M,Yokoi N,Miki T,Horikawa Y,Yoshioka H,Takeda J,Ohara O,Seino S

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Ibtk cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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