Xrcc4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Xrcc4
Entrez Gene ID	108138
Full Name	X-ray repair complementing defective repair in Chinese hamster cells 4
Synonyms	2310057B22Rik,AW413319,AW545101
General protein information	Preferred Names X-ray repair complementing defective repair in Chinese hamster cells 4 Names DNA repair protein XRCC4 X-ray repair cross-complementing protein 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 C3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006517041.4	XP_006517104.1	DNA repair protein XRCC4 isoform X1
XM_006517043.4	XP_006517106.1	DNA repair protein XRCC4 isoform X1
XM_006517042.2	XP_006517105.1	DNA repair protein XRCC4 isoform X1
XM_006517041.3	XP_006517104.1	DNA repair protein XRCC4 isoform X1
XM_006517043.3	XP_006517106.1	DNA repair protein XRCC4 isoform X1
NM_028012.4	NP_082288.1	DNA repair protein XRCC4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	XRCC4	NP_001075084.1
Rattus norvegicus (Norway rat)	Xrcc4	NP_001007000.1
Pan troglodytes (chimpanzee)	XRCC4	XP_001148110.2
	LOC712291	XP_001111862.1
Mus musculus (house mouse)	Xrcc4	NP_082288.1
Danio rerio (zebrafish)	xrcc4	NP_957080.1
Gallus gallus (chicken)	XRCC4	XP_424905.4
Xenopus tropicalis (tropical clawed frog)	xrcc4	NP_001106551.1
Homo sapiens (human)	XRCC4	NP_071801.1
Canis lupus familiaris (dog)	XRCC4	XP_005618208.1

Gene Ontology
Bibliography

Related articles in PubMed

PAXX and Xlf interplay revealed by impaired CNS development and immunodeficiency of double KO mice.
Abramowski V, Etienne O, Elsaid R, Yang J, Berland A, Kermasson L, Roch B, Musilli S, Moussu JP, Lipson-Ruffert K, Revy P, Cumano A, Boussin FD, de Villartay JP
Cell death and differentiation25(2)444-452(2018 Feb)

Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase.
Sunatani Y, Kamdar RP, Sharma MK, Matsui T, Sakasai R, Hashimoto M, Ishigaki Y, Matsumoto Y, Iwabuchi K
Experimental cell research362(2)450-460(2018 Jan)

Synthetic lethality between murine DNA repair factors XLF and DNA-PKcs is rescued by inactivation of Ku70.
Xing M, Bj?r?s M, Daniel JA, Alt FW, Oksenych V
DNA repair57133-138(2017 Sep)

Germline Chd8 haploinsufficiency alters brain development in mouse.
Gompers AL, Su-Feher L, Ellegood J, Copping NA, Riyadh MA, Stradleigh TW, Pride MC, Schaffler MD, Wade AA, Catta-Preta R, Zdilar I, Louis S, Kaushik G, Mannion BJ, Plajzer-Frick I, Afzal V, Visel A, Pennacchio LA, Dickel DE, Lerch JP, Crawley JN, Zarbalis KS, Silverman JL, Nord AS
Nature neuroscience20(8)1062-1073(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We find that non-canonical HR termination can occur in the absence of the classical non-homologous end joining gene XRCC4. We observe obligatory use of microhomology (MH)-mediated end joining and/or nucleotide addition during rejoining with the second end of the break
Title: Complex Breakpoints and Template Switching Associated with Non-canonical Termination of Homologous Recombination in Mammalian Cells.

involvement of ZNF281 in the cellular response to genotoxic stress through the control exercised on the expression of genes that act in different repair mechanisms
Title: ZNF281 contributes to the DNA damage response by controlling the expression of XRCC2 and XRCC4.

FBXW7 facilitates nonhomologous end-joining via K63-linked polyubiquitylation of XRCC4 in tumor cells.
Title: FBXW7 Facilitates Nonhomologous End-Joining via K63-Linked Polyubiquitylation of XRCC4.

Data show that combined deletion of X-ray repair cross-complementing protein 4 (Xrcc4) and tumor suppressor p53 (Trp53) predisposes B cells to lymphomagenesis.
Title: Combined deletion of Xrcc4 and Trp53 in mouse germinal center B cells leads to novel B cell lymphomas with clonal heterogeneity.

The present results collectively indicated that Lys271, but not Lys210, of XRCC4 is required for the nuclear localization of XRCC4 and LIG4 and that the nuclear localizing ability is essential for DSB repair function of XRCC4.
Title: Lysine 271 but not lysine 210 of XRCC4 is required for the nuclear localization of XRCC4 and DNA ligase IV.

The following Xrcc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Xrcc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04954	NM_028012.4 Latest version!	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu04954	XM_006517042.2 Latest version!	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu04954	XM_006517041.4 Latest version!	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu04954	XM_006517043.4 Latest version!	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu04954	XM_006517041.3	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu04954	XM_006517043.3	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Xrcc4 ( NM_028012.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082288.1 Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), mRNA.

Price & Availability↑

CloneID	OMu04954
Clone ID Related Accession (Same CDS sequence)	XM_006517041.4 , XM_006517043.4 , XM_006517042.2 , XM_006517041.3 , XM_006517043.3 , NM_028012.4
Accession Version	NM_028012.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	DNA repair protein XRCC4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY774472.1, AK009951.1 and AW455986.1. On or before Feb 15, 2015 this sequence version replaced XM_011245793.1, XM_006543098.1, XM_003689283.1, XM_003689284.1, XM_003689285.1, XM_003689286.1, XM_003689287.1, XM_003689288.1, NM_028012.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB055154.1, AK088281.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082288.1
CDS	123..1103
Translation	MERKVSRIYLASEPNVPYFLQVSWERTIGSGFVITLTDGHSAWTATVSELEISQEADDMAMEKGKYIDELRKALVPGSGAAGTYKFLFSKESRHFSLEKELKDVSFRLGSFNLDKVSNSAEVIRDLICYCLDTITEKQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALEADLYQRFILVLNEKKTKIRSLHKLLNEVQQLEESTKPERENPCSDKTPEEHGLYDGSTDEESGAPVQAAETLHKDDSIFSSPDVTDIAPSRKRRHRMQKNLGTEPKMAPQELPLQEKERLASSLPQTLKEESTSAENMSLETLRNSSPEDLFD

Target ORF information:

RefSeq Version	NM_028012.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028012.4

ORF Insert Sequence:

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Xrcc4 ( XM_006517042.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517105.1 Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu04954
Clone ID Related Accession (Same CDS sequence)	XM_006517041.4 , XM_006517043.4 , XM_006517042.2 , XM_006517041.3 , XM_006517043.3 , NM_028012.4
Accession Version	XM_006517042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA repair protein XRCC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517105.1
CDS	101..1081
Translation	MERKVSRIYLASEPNVPYFLQVSWERTIGSGFVITLTDGHSAWTATVSELEISQEADDMAMEKGKYIDELRKALVPGSGAAGTYKFLFSKESRHFSLEKELKDVSFRLGSFNLDKVSNSAEVIRDLICYCLDTITEKQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALEADLYQRFILVLNEKKTKIRSLHKLLNEVQQLEESTKPERENPCSDKTPEEHGLYDGSTDEESGAPVQAAETLHKDDSIFSSPDVTDIAPSRKRRHRMQKNLGTEPKMAPQELPLQEKERLASSLPQTLKEESTSAENMSLETLRNSSPEDLFD

Target ORF information:

RefSeq Version	XM_006517042.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517042.2

ORF Insert Sequence:

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Xrcc4 ( XM_006517041.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517104.1 Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu04954
Clone ID Related Accession (Same CDS sequence)	XM_006517041.4 , XM_006517043.4 , XM_006517042.2 , XM_006517041.3 , XM_006517043.3 , NM_028012.4
Accession Version	XM_006517041.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA repair protein XRCC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517041.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517104.1
CDS	94..1074
Translation	MERKVSRIYLASEPNVPYFLQVSWERTIGSGFVITLTDGHSAWTATVSELEISQEADDMAMEKGKYIDELRKALVPGSGAAGTYKFLFSKESRHFSLEKELKDVSFRLGSFNLDKVSNSAEVIRDLICYCLDTITEKQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALEADLYQRFILVLNEKKTKIRSLHKLLNEVQQLEESTKPERENPCSDKTPEEHGLYDGSTDEESGAPVQAAETLHKDDSIFSSPDVTDIAPSRKRRHRMQKNLGTEPKMAPQELPLQEKERLASSLPQTLKEESTSAENMSLETLRNSSPEDLFD

Target ORF information:

RefSeq Version	XM_006517041.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517041.3

ORF Insert Sequence:

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Xrcc4 ( XM_006517043.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517106.1 Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu04954
Clone ID Related Accession (Same CDS sequence)	XM_006517041.4 , XM_006517043.4 , XM_006517042.2 , XM_006517041.3 , XM_006517043.3 , NM_028012.4
Accession Version	XM_006517043.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	DNA repair protein XRCC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006517043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517106.1
CDS	96..1076
Translation	MERKVSRIYLASEPNVPYFLQVSWERTIGSGFVITLTDGHSAWTATVSELEISQEADDMAMEKGKYIDELRKALVPGSGAAGTYKFLFSKESRHFSLEKELKDVSFRLGSFNLDKVSNSAEVIRDLICYCLDTITEKQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALEADLYQRFILVLNEKKTKIRSLHKLLNEVQQLEESTKPERENPCSDKTPEEHGLYDGSTDEESGAPVQAAETLHKDDSIFSSPDVTDIAPSRKRRHRMQKNLGTEPKMAPQELPLQEKERLASSLPQTLKEESTSAENMSLETLRNSSPEDLFD

Target ORF information:

RefSeq Version	XM_006517043.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517043.3

ORF Insert Sequence:

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Xrcc4 ( XM_006517041.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517104.1 Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X1, mRNA.

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CloneID	OMu04954
Clone ID Related Accession (Same CDS sequence)	XM_006517041.4 , XM_006517043.4 , XM_006517042.2 , XM_006517041.3 , XM_006517043.3 , NM_028012.4
Accession Version	XM_006517041.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA repair protein XRCC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517041.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517104.1
CDS	481..1461
Translation	MERKVSRIYLASEPNVPYFLQVSWERTIGSGFVITLTDGHSAWTATVSELEISQEADDMAMEKGKYIDELRKALVPGSGAAGTYKFLFSKESRHFSLEKELKDVSFRLGSFNLDKVSNSAEVIRDLICYCLDTITEKQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALEADLYQRFILVLNEKKTKIRSLHKLLNEVQQLEESTKPERENPCSDKTPEEHGLYDGSTDEESGAPVQAAETLHKDDSIFSSPDVTDIAPSRKRRHRMQKNLGTEPKMAPQELPLQEKERLASSLPQTLKEESTSAENMSLETLRNSSPEDLFD

Target ORF information:

RefSeq Version	XM_006517041.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517041.4

ORF Insert Sequence:

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Xrcc4 ( XM_006517043.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006517106.1 Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu04954
Clone ID Related Accession (Same CDS sequence)	XM_006517041.4 , XM_006517043.4 , XM_006517042.2 , XM_006517041.3 , XM_006517043.3 , NM_028012.4
Accession Version	XM_006517043.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	DNA repair protein XRCC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006517043.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006517106.1
CDS	139..1119
Translation	MERKVSRIYLASEPNVPYFLQVSWERTIGSGFVITLTDGHSAWTATVSELEISQEADDMAMEKGKYIDELRKALVPGSGAAGTYKFLFSKESRHFSLEKELKDVSFRLGSFNLDKVSNSAEVIRDLICYCLDTITEKQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALEADLYQRFILVLNEKKTKIRSLHKLLNEVQQLEESTKPERENPCSDKTPEEHGLYDGSTDEESGAPVQAAETLHKDDSIFSSPDVTDIAPSRKRRHRMQKNLGTEPKMAPQELPLQEKERLASSLPQTLKEESTSAENMSLETLRNSSPEDLFD

Target ORF information:

RefSeq Version	XM_006517043.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006517043.4

ORF Insert Sequence:

ATGGAAAGGA AAGTAAGCAG AATCTATCTT GCTTCTGAAC CCAACGTACC TTATTTTCTG 
CAAGTGTCTT GGGAGAGAAC AATAGGATCC GGCTTTGTTA TTACACTTAC TGACGGCCAT 
TCAGCCTGGA CTGCAACAGT TTCTGAATTG GAGATTTCCC AAGAAGCTGA TGACATGGCT 
ATGGAGAAAG GAAAATACAT TGATGAGCTG AGAAAGGCAC TGGTGCCAGG GTCAGGAGCA 
GCTGGTACAT ACAAGTTCCT CTTTTCTAAG GAGTCTCGGC ATTTCTCCCT TGAGAAAGAA 
CTGAAAGATG TTTCATTCAG ACTTGGTTCC TTCAACCTAG ACAAAGTTTC AAACTCAGCT 
GAAGTCATAA GAGATCTCAT TTGTTACTGC CTGGACACCA TTACAGAAAA GCAAGCCAAA 
AACGAGCACC TGCAGAAAGA AAATGAAAGG CTCCTGAGAG ACTGGAATGA TGTTCAAGGC 
CGATTTGAGA AATGTGTGAG TGCCAAAGAA GCCTTGGAGG CTGATTTGTA TCAAAGATTT 
ATCTTGGTGC TGAATGAGAA GAAAACAAAG ATCCGGAGCT TGCATAAATT GCTAAATGAA 
GTCCAGCAGC TGGAGGAGAG TACCAAACCT GAAAGGGAAA ATCCGTGTTC TGACAAAACA 
CCTGAGGAGC ATGGGCTTTA TGATGGAAGC ACCGATGAAG AAAGTGGAGC CCCTGTGCAG 
GCTGCAGAAA CTCTTCATAA GGATGATTCC ATATTTTCAA GTCCGGATGT CACTGACATT 
GCACCAAGTA GGAAGAGGAG ACACCGAATG CAGAAGAATC TTGGGACAGA ACCTAAAATG 
GCTCCACAGG AGTTGCCGCT ACAGGAGAAG GAAAGGCTTG CCTCTTCACT ACCCCAGACC 
TTGAAAGAGG AGAGCACCTC TGCTGAAAAC ATGTCTTTAG AAACCCTGAG AAACAGCAGC 
CCAGAAGATC TCTTTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PAXX and Xlf interplay revealed by impaired CNS development and immunodeficiency of double KO mice.
Cell death and differentiation25(2)444-452(2018 Feb)
Abramowski V,Etienne O,Elsaid R,Yang J,Berland A,Kermasson L,Roch B,Musilli S,Moussu JP,Lipson-Ruffert K,Revy P,Cumano A,Boussin FD,de Villartay JP

Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase.
Experimental cell research362(2)450-460(2018 Jan)
Sunatani Y,Kamdar RP,Sharma MK,Matsui T,Sakasai R,Hashimoto M,Ishigaki Y,Matsumoto Y,Iwabuchi K

Synthetic lethality between murine DNA repair factors XLF and DNA-PKcs is rescued by inactivation of Ku70.
DNA repair57133-138(2017 Sep)
Xing M,Bj?r?s M,Daniel JA,Alt FW,Oksenych V

Germline Chd8 haploinsufficiency alters brain development in mouse.
Nature neuroscience20(8)1062-1073(2017 Aug)
Gompers AL,Su-Feher L,Ellegood J,Copping NA,Riyadh MA,Stradleigh TW,Pride MC,Schaffler MD,Wade AA,Catta-Preta R,Zdilar I,Louis S,Kaushik G,Mannion BJ,Plajzer-Frick I,Afzal V,Visel A,Pennacchio LA,Dickel DE,Lerch JP,Crawley JN,Zarbalis KS,Silverman JL,Nord AS

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Xrcc4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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