U2af1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	U2af1
Entrez Gene ID	108121
Full Name	U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1
Synonyms	2010107D16Rik
General protein information	Preferred Names U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 Names splicing factor U2AF 35 kDa subunit U2 auxiliary factor 35 kDa subunit U2 snRNP auxiliary factor small subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 B1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006523472.1	XP_006523535.1	splicing factor U2AF 35 kDa subunit isoform X1
XM_030249444.1	XP_030105304.1	splicing factor U2AF 35 kDa subunit isoform X1
NM_001163769.1	NP_001157241.1	splicing factor U2AF 35 kDa subunit isoform 2
XM_006523472.2	XP_006523535.1	splicing factor U2AF 35 kDa subunit isoform X1
NM_001357907.1	NP_001344836.1	splicing factor U2AF 35 kDa subunit isoform 3
XM_006523473.2	XP_006523536.1	splicing factor U2AF 35 kDa subunit isoform X1
NM_024187.4	NP_077149.2	splicing factor U2AF 35 kDa subunit isoform 1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPAP8A3.06	NP_594945.1
Pan troglodytes (chimpanzee)	U2AF1	XP_003949235.1
Canis lupus familiaris (dog)	U2AF1	XP_003640135.1
Bos taurus (cattle)	U2AF1	NP_001073737.1
Rattus norvegicus (Norway rat)	U2af1	NP_001037756.1
Drosophila melanogaster (fruit fly)	U2af38	NP_477208.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002956	XP_311943.3
Magnaporthe oryzae (rice blast fungus)	MGG_09948	XP_003710052.1
Oryza sativa (rice)	Os01g0667800	NP_001043811.2
Mus musculus (house mouse)	U2af1	NP_077149.2
Caenorhabditis elegans (roundworm)	uaf-2	NP_503036.1
Xenopus tropicalis (tropical clawed frog)	u2af1	NP_001039113.1
	LOC722383	XP_001118538.2
Gallus gallus (chicken)	U2AF1	NP_989986.2
Neurospora crassa	NCU03261	XP_964414.1

Gene Ontology
Bibliography

Related articles in PubMed

U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3' End Formation.
Park SM, Ou J, Chamberlain L, Simone TM, Yang H, Virbasius CM, Ali AM, Zhu LJ, Mukherjee S, Raza A, Green MR
Molecular cell62(4)479-90(2016 05)

Mutant U2AF1 Expression Alters Hematopoiesis and Pre-mRNA Splicing In Vivo.
Shirai CL, Ley JN, White BS, Kim S, Tibbitts J, Shao J, Ndonwi M, Wadugu B, Duncavage EJ, Okeyo-Owuor T, Liu T, Griffith M, McGrath S, Magrini V, Fulton RS, Fronick C, O'Laughlin M, Graubert TA, Walter MJ
Cancer cell27(5)631-43(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The U2AF35(S34F) mutation alters interaction with CFIm59, leading to increased use of a distal cleavage and polyadenylation site in the ATG7 pre-mRNA, decreasing levels of ATG7 protein and defective autophagy, ultimately leading to transformation.
Title: U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3' End Formation.

findings support the hypothesis that mutant U2AF1 alters downstream gene isoform expression, thereby contributing to abnormal hematopoiesis in patients with MDS
Title: Mutant U2AF1 Expression Alters Hematopoiesis and Pre-mRNA Splicing In Vivo.

data support the impact of genes from the Abcg1-U2af1 region as modifiers of Tc1-dependent memory and locomotor phenotypes in Tc1 mouse model of Down syndrome.
Title: Dosage of the Abcg1-U2af1 region modifies locomotor and cognitive deficits observed in the Tc1 mouse model of Down syndrome.

this study establishes definitely the contribution of the Abcg1-U2af1 orthologous region to the DS etiology and suggests new modulatory pathways for learning and memory.
Title: A new mouse model for the trisomy of the Abcg1-U2af1 region reveals the complexity of the combinatorial genetic code of down syndrome.

The following U2af1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the U2af1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu52999	NM_001357907.1 Latest version!	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu52999	XM_006523473.2 Latest version!	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu09421	NM_001163769.1 Latest version!	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu01283	NM_024187.4 Latest version!	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu52999	XM_030249444.1 Latest version!	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu52999	XM_006523472.2 Latest version!	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu52999	XM_006523472.1	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

U2af1 ( NM_001357907.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344836.1 Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu52999
Clone ID Related Accession (Same CDS sequence)	XM_006523472.1 , XM_030249444.1 , XM_006523472.2 , NM_001357907.1 , XM_006523473.2
Accession Version	NM_001357907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Mus musculus(house mouse)
Product	splicing factor U2AF 35 kDa subunit isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC090881.9 and BC069888.1. On Nov 10, 2017 this sequence version replaced XM_006523473.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.372872.1, AV476937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGAGC ACTATGATGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAAGTCG AGGAGATGAA CGTCTGCGAC AACCTAGGGG ACCACCTGGT GGGGAACGTG 
TATGTCAAGT TTCGACGTGA GGAGGATGCA GAGAAAGCCG TGATCGACTT GAATAACCGT 
TGGTTTAATG GACAGCCGAT CCATGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCC 
TGCTGCCGCC AGTATGAAAT GGGAGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
TTGAAGCCCA TCTCAAGAGA GCTACGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAACGGCGT TCCCGATCCA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGTGG CGGAGGACGG GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GACGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344836.1
CDS	133..633
Translation	MQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSGRF

Target ORF information:

RefSeq Version	NM_001357907.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357907.1

ORF Insert Sequence:

ATGCAGGAGC ACTATGATGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAAGTCG AGGAGATGAA CGTCTGCGAC AACCTAGGGG ACCACCTGGT GGGGAACGTG 
TATGTCAAGT TTCGACGTGA GGAGGATGCA GAGAAAGCCG TGATCGACTT GAATAACCGT 
TGGTTTAATG GACAGCCGAT CCATGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCC 
TGCTGCCGCC AGTATGAAAT GGGAGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
TTGAAGCCCA TCTCAAGAGA GCTACGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAACGGCGT TCCCGATCCA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGTGG CGGAGGACGG GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GACGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2af1 ( XM_006523472.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523535.1 Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu52999
Clone ID Related Accession (Same CDS sequence)	XM_006523472.1 , XM_030249444.1 , XM_006523472.2 , NM_001357907.1 , XM_006523473.2
Accession Version	XM_006523472.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	splicing factor U2AF 35 kDa subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAGC ACTATGATGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAAGTCG AGGAGATGAA CGTCTGCGAC AACCTAGGGG ACCACCTGGT GGGGAACGTG 
TATGTCAAGT TTCGACGTGA GGAGGATGCA GAGAAAGCCG TGATCGACTT GAATAACCGT 
TGGTTTAATG GACAGCCGAT CCATGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCC 
TGCTGCCGCC AGTATGAAAT GGGAGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
TTGAAGCCCA TCTCAAGAGA GCTACGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAACGGCGT TCCCGATCCA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGTGG CGGAGGACGG GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GACGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523535.1
CDS	355..855
Translation	MQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSGRF

Target ORF information:

RefSeq Version	XM_006523472.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523472.1

ORF Insert Sequence:

ATGCAGGAGC ACTATGATGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAAGTCG AGGAGATGAA CGTCTGCGAC AACCTAGGGG ACCACCTGGT GGGGAACGTG 
TATGTCAAGT TTCGACGTGA GGAGGATGCA GAGAAAGCCG TGATCGACTT GAATAACCGT 
TGGTTTAATG GACAGCCGAT CCATGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCC 
TGCTGCCGCC AGTATGAAAT GGGAGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
TTGAAGCCCA TCTCAAGAGA GCTACGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAACGGCGT TCCCGATCCA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGTGG CGGAGGACGG GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GACGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2af1 ( XM_006523473.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523536.1 Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu52999
Clone ID Related Accession (Same CDS sequence)	XM_006523472.1 , XM_030249444.1 , XM_006523472.2 , NM_001357907.1 , XM_006523473.2
Accession Version	XM_006523473.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	splicing factor U2AF 35 kDa subunit isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523473.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAGC ACTATGATGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAAGTCG AGGAGATGAA CGTCTGCGAC AACCTAGGGG ACCACCTGGT GGGGAACGTG 
TATGTCAAGT TTCGACGTGA GGAGGATGCA GAGAAAGCCG TGATCGACTT GAATAACCGT 
TGGTTTAATG GACAGCCGAT CCATGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCC 
TGCTGCCGCC AGTATGAAAT GGGAGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
TTGAAGCCCA TCTCAAGAGA GCTACGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAACGGCGT TCCCGATCCA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGTGG CGGAGGACGG GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GACGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523536.1
CDS	111..611
Translation	MQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSGRF

Target ORF information:

RefSeq Version	XM_006523473.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523473.2

ORF Insert Sequence:

ATGCAGGAGC ACTATGATGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAAGTCG AGGAGATGAA CGTCTGCGAC AACCTAGGGG ACCACCTGGT GGGGAACGTG 
TATGTCAAGT TTCGACGTGA GGAGGATGCA GAGAAAGCCG TGATCGACTT GAATAACCGT 
TGGTTTAATG GACAGCCGAT CCATGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCC 
TGCTGCCGCC AGTATGAAAT GGGAGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
TTGAAGCCCA TCTCAAGAGA GCTACGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAACGGCGT TCCCGATCCA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGTGG CGGAGGACGG GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GACGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2af1 ( NM_001163769.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157241.1 Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09421
Clone ID Related Accession (Same CDS sequence)	NM_001163769.1
Accession Version	NM_001163769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Mus musculus(house mouse)
Product	splicing factor U2AF 35 kDa subunit isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK150872.1 and AV453783.1. Transcript Variant: This variant (2) lacks an alternate coding exon but contains another compared to variant 1. Variants 1 and 2 are the same length, as are isoforms 1 and 2; however, their sequences vary slightly. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CA977261.1, CB195159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAT ACTTGGCCTC CATCTTCGGC ACCGAAAAAG ACAAAGTCAA CTGTTCATTT 
TATTTCAAAA TCGGAGCATG TCGTCATGGA GACAGATGTT CTCGGTTGCA CAATAAACCA 
ACCTTTAGCC AGACCATCTT GATTCAAAAC ATCTATCGTA ATCCCCAAAA CAGTGCACAG 
ACGGCTGACG GCTCACACTG TGCTGTGAGC GACGTGGAGA TGCAGGAGCA CTATGATGAG 
TTCTTTGAGG AAGTCTTCAC TGAGATGGAA GAGAAGTACG GGGAAGTCGA GGAGATGAAC 
GTCTGCGACA ACCTAGGGGA CCACCTGGTG GGGAACGTGT ATGTCAAGTT TCGACGTGAG 
GAGGATGCAG AGAAAGCCGT GATCGACTTG AATAACCGTT GGTTTAATGG ACAGCCGATC 
CATGCAGAGC TGTCCCCAGT AACTGACTTC AGGGAAGCCT GCTGCCGCCA GTATGAAATG 
GGAGAGTGCA CAAGAGGGGG CTTCTGCAAC TTCATGCATT TGAAGCCCAT CTCAAGAGAG 
CTACGACGGG AGCTGTATGG GCGCCGGCGC AAGAAGCATA GATCCAGGTC CCGATCCAGG 
GAACGGCGTT CCCGATCCAG AGACCGTGGA CGCGGTGGTG GAGGTGGAGG TGGAGGTGGC 
GGAGGACGGG AGCGTGACAG GAGGCGGTCA AGAGACCGGG AGAGATCTGG ACGATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157241.1
CDS	59..778
Translation	MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTILIQNIYRNPQNSAQTADGSHCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSGRF

Target ORF information:

RefSeq Version	NM_001163769.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163769.1

ORF Insert Sequence:

ATGGCGGAAT ACTTGGCCTC CATCTTCGGC ACCGAAAAAG ACAAAGTCAA CTGTTCATTT 
TATTTCAAAA TCGGAGCATG TCGTCATGGA GACAGATGTT CTCGGTTGCA CAATAAACCA 
ACCTTTAGCC AGACCATCTT GATTCAAAAC ATCTATCGTA ATCCCCAAAA CAGTGCACAG 
ACGGCTGACG GCTCACACTG TGCTGTGAGC GACGTGGAGA TGCAGGAGCA CTATGATGAG 
TTCTTTGAGG AAGTCTTCAC TGAGATGGAA GAGAAGTACG GGGAAGTCGA GGAGATGAAC 
GTCTGCGACA ACCTAGGGGA CCACCTGGTG GGGAACGTGT ATGTCAAGTT TCGACGTGAG 
GAGGATGCAG AGAAAGCCGT GATCGACTTG AATAACCGTT GGTTTAATGG ACAGCCGATC 
CATGCAGAGC TGTCCCCAGT AACTGACTTC AGGGAAGCCT GCTGCCGCCA GTATGAAATG 
GGAGAGTGCA CAAGAGGGGG CTTCTGCAAC TTCATGCATT TGAAGCCCAT CTCAAGAGAG 
CTACGACGGG AGCTGTATGG GCGCCGGCGC AAGAAGCATA GATCCAGGTC CCGATCCAGG 
GAACGGCGTT CCCGATCCAG AGACCGTGGA CGCGGTGGTG GAGGTGGAGG TGGAGGTGGC 
GGAGGACGGG AGCGTGACAG GAGGCGGTCA AGAGACCGGG AGAGATCTGG ACGATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2af1 ( NM_024187.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077149.2 Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01283
Clone ID Related Accession (Same CDS sequence)	NM_024187.4
Accession Version	NM_024187.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Mus musculus(house mouse)
Product	splicing factor U2AF 35 kDa subunit isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK150872.1. On Jul 31, 2009 this sequence version replaced NM_024187.3. Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 2 are the same length, as are isoforms 1 and 2; however, their sequences vary slightly. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK150872.1, CA979558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAAT ACTTGGCCTC CATCTTCGGC ACCGAAAAAG ACAAAGTCAA CTGTTCATTT 
TATTTCAAAA TCGGAGCATG TCGTCATGGA GACAGATGTT CTCGGTTGCA CAATAAACCA 
ACCTTTAGCC AGACCATTGC CCTCTTGAAC ATTTACCGTA ACCCTCAAAA CTCTTCCCAG 
TCTGCTGACG GTTTGCGCTG TGCTGTGAGC GACGTGGAGA TGCAGGAGCA CTATGATGAG 
TTCTTTGAGG AAGTCTTCAC TGAGATGGAA GAGAAGTACG GGGAAGTCGA GGAGATGAAC 
GTCTGCGACA ACCTAGGGGA CCACCTGGTG GGGAACGTGT ATGTCAAGTT TCGACGTGAG 
GAGGATGCAG AGAAAGCCGT GATCGACTTG AATAACCGTT GGTTTAATGG ACAGCCGATC 
CATGCAGAGC TGTCCCCAGT AACTGACTTC AGGGAAGCCT GCTGCCGCCA GTATGAAATG 
GGAGAGTGCA CAAGAGGGGG CTTCTGCAAC TTCATGCATT TGAAGCCCAT CTCAAGAGAG 
CTACGACGGG AGCTGTATGG GCGCCGGCGC AAGAAGCATA GATCCAGGTC CCGATCCAGG 
GAACGGCGTT CCCGATCCAG AGACCGTGGA CGCGGTGGTG GAGGTGGAGG TGGAGGTGGC 
GGAGGACGGG AGCGTGACAG GAGGCGGTCA AGAGACCGGG AGAGATCTGG ACGATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077149.2
CDS	59..778
Translation	MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQSADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSGRF

Target ORF information:

RefSeq Version	NM_024187.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024187.4

ORF Insert Sequence:

ATGGCGGAAT ACTTGGCCTC CATCTTCGGC ACCGAAAAAG ACAAAGTCAA CTGTTCATTT 
TATTTCAAAA TCGGAGCATG TCGTCATGGA GACAGATGTT CTCGGTTGCA CAATAAACCA 
ACCTTTAGCC AGACCATTGC CCTCTTGAAC ATTTACCGTA ACCCTCAAAA CTCTTCCCAG 
TCTGCTGACG GTTTGCGCTG TGCTGTGAGC GACGTGGAGA TGCAGGAGCA CTATGATGAG 
TTCTTTGAGG AAGTCTTCAC TGAGATGGAA GAGAAGTACG GGGAAGTCGA GGAGATGAAC 
GTCTGCGACA ACCTAGGGGA CCACCTGGTG GGGAACGTGT ATGTCAAGTT TCGACGTGAG 
GAGGATGCAG AGAAAGCCGT GATCGACTTG AATAACCGTT GGTTTAATGG ACAGCCGATC 
CATGCAGAGC TGTCCCCAGT AACTGACTTC AGGGAAGCCT GCTGCCGCCA GTATGAAATG 
GGAGAGTGCA CAAGAGGGGG CTTCTGCAAC TTCATGCATT TGAAGCCCAT CTCAAGAGAG 
CTACGACGGG AGCTGTATGG GCGCCGGCGC AAGAAGCATA GATCCAGGTC CCGATCCAGG 
GAACGGCGTT CCCGATCCAG AGACCGTGGA CGCGGTGGTG GAGGTGGAGG TGGAGGTGGC 
GGAGGACGGG AGCGTGACAG GAGGCGGTCA AGAGACCGGG AGAGATCTGG ACGATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2af1 ( XM_030249444.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105304.1 Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X2, mRNA.

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CloneID	OMu52999
Clone ID Related Accession (Same CDS sequence)	XM_006523472.1 , XM_030249444.1 , XM_006523472.2 , NM_001357907.1 , XM_006523473.2
Accession Version	XM_030249444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	splicing factor U2AF 35 kDa subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAGC ACTATGATGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAAGTCG AGGAGATGAA CGTCTGCGAC AACCTAGGGG ACCACCTGGT GGGGAACGTG 
TATGTCAAGT TTCGACGTGA GGAGGATGCA GAGAAAGCCG TGATCGACTT GAATAACCGT 
TGGTTTAATG GACAGCCGAT CCATGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCC 
TGCTGCCGCC AGTATGAAAT GGGAGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
TTGAAGCCCA TCTCAAGAGA GCTACGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAACGGCGT TCCCGATCCA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGTGG CGGAGGACGG GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GACGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105304.1
CDS	203..703
Translation	MQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSGRF

Target ORF information:

RefSeq Version	XM_030249444.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249444.1

ORF Insert Sequence:

ATGCAGGAGC ACTATGATGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAAGTCG AGGAGATGAA CGTCTGCGAC AACCTAGGGG ACCACCTGGT GGGGAACGTG 
TATGTCAAGT TTCGACGTGA GGAGGATGCA GAGAAAGCCG TGATCGACTT GAATAACCGT 
TGGTTTAATG GACAGCCGAT CCATGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCC 
TGCTGCCGCC AGTATGAAAT GGGAGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
TTGAAGCCCA TCTCAAGAGA GCTACGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAACGGCGT TCCCGATCCA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGTGG CGGAGGACGG GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GACGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2af1 ( XM_006523472.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523535.1 Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X1, mRNA.

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CloneID	OMu52999
Clone ID Related Accession (Same CDS sequence)	XM_006523472.1 , XM_030249444.1 , XM_006523472.2 , NM_001357907.1 , XM_006523473.2
Accession Version	XM_006523472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	splicing factor U2AF 35 kDa subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAGC ACTATGATGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAAGTCG AGGAGATGAA CGTCTGCGAC AACCTAGGGG ACCACCTGGT GGGGAACGTG 
TATGTCAAGT TTCGACGTGA GGAGGATGCA GAGAAAGCCG TGATCGACTT GAATAACCGT 
TGGTTTAATG GACAGCCGAT CCATGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCC 
TGCTGCCGCC AGTATGAAAT GGGAGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
TTGAAGCCCA TCTCAAGAGA GCTACGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAACGGCGT TCCCGATCCA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGTGG CGGAGGACGG GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GACGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523535.1
CDS	358..858
Translation	MQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSGRF

Target ORF information:

RefSeq Version	XM_006523472.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1 (U2af1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523472.2

ORF Insert Sequence:

ATGCAGGAGC ACTATGATGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAAGTCG AGGAGATGAA CGTCTGCGAC AACCTAGGGG ACCACCTGGT GGGGAACGTG 
TATGTCAAGT TTCGACGTGA GGAGGATGCA GAGAAAGCCG TGATCGACTT GAATAACCGT 
TGGTTTAATG GACAGCCGAT CCATGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCC 
TGCTGCCGCC AGTATGAAAT GGGAGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
TTGAAGCCCA TCTCAAGAGA GCTACGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAACGGCGT TCCCGATCCA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGTGG CGGAGGACGG GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GACGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3' End Formation.
Molecular cell62(4)479-90(2016 05)
Park SM,Ou J,Chamberlain L,Simone TM,Yang H,Virbasius CM,Ali AM,Zhu LJ,Mukherjee S,Raza A,Green MR

Mutant U2AF1 Expression Alters Hematopoiesis and Pre-mRNA Splicing In Vivo.
Cancer cell27(5)631-43(2015 May)
Shirai CL,Ley JN,White BS,Kim S,Tibbitts J,Shao J,Ndonwi M,Wadugu B,Duncavage EJ,Okeyo-Owuor T,Liu T,Griffith M,McGrath S,Magrini V,Fulton RS,Fronick C,O'Laughlin M,Graubert TA,Walter MJ

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U2af1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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