Shmt2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shmt2
Entrez Gene ID	108037
Full Name	serine hydroxymethyltransferase 2 (mitochondrial)
Synonyms	2700043D08Rik,AA408223,AA986903
General protein information	Preferred Names serine hydroxymethyltransferase 2 (mitochondrial) Names serine hydroxymethyltransferase, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001252316.1	NP_001239245.1	serine hydroxymethyltransferase, mitochondrial isoform 2
NM_028230.4	NP_082506.1	serine hydroxymethyltransferase, mitochondrial isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Shmt2	NP_082506.1
Rattus norvegicus (Norway rat)	Shmt2	NP_001008323.1
Saccharomyces cerevisiae (baker's yeast)	SHM1	NP_009822.4
Neurospora crassa	NCU05805	XP_960065.1
Pan troglodytes (chimpanzee)	SHMT2	XP_509157.3
Canis lupus familiaris (dog)	SHMT2	XP_849244.1
Kluyveromyces lactis	KLLA0F01210g	XP_455134.1
	SHMT2	XP_001115892.2
Danio rerio (zebrafish)	shmt2	NP_001116846.1
Eremothecium gossypii	AGOS_AEL188W	NP_984673.1
Schizosaccharomyces pombe (fission yeast)	shm2	NP_593668.2
Arabidopsis thaliana (thale cress)	SHM1	NP_195506.1
Homo sapiens (human)	SHMT2	NP_005403.2
Bos taurus (cattle)	SHMT2	NP_001029454.1
Magnaporthe oryzae (rice blast fungus)	MGG_13781	XP_003710851.1
Oryza sativa (rice)	Os03g0738400	NP_001051211.1
Xenopus tropicalis (tropical clawed frog)	shmt2	NP_001007880.1

Gene Ontology
Bibliography

Related articles in PubMed

Mice deficient in the Shmt2 gene have mitochondrial respiration defects and are embryonic lethal.
Tani H, Ohnishi S, Shitara H, Mito T, Yamaguchi M, Yonekawa H, Hashizume O, Ishikawa K, Nakada K, Hayashi JI
Scientific reports8(1)425(2018 Jan)

Karyopherin Alpha 1 Regulates Satellite Cell Proliferation and Survival by Modulating Nuclear Import.
Choo HJ, Cutler A, Rother F, Bader M, Pavlath GK
Stem cells (Dayton, Ohio)34(11)2784-2797(2016 Nov)

SHMT1 and SHMT2 are functionally redundant in nuclear de novo thymidylate biosynthesis.
Anderson DD, Stover PJ
PloS one4(6)e5839(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SHMT1 and SHMT2 are functionally redundant in nuclear de novo thymidylate biosynthesis
Title: SHMT1 and SHMT2 are functionally redundant in nuclear de novo thymidylate biosynthesis.

The following Shmt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shmt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04964	NM_001252316.1 Latest version!	Mus musculus serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu05369	NM_028230.4 Latest version!	Mus musculus serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shmt2 ( NM_001252316.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239245.1 Mus musculus serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu04964
Clone ID Related Accession (Same CDS sequence)	NM_001252316.1
Accession Version	NM_001252316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	serine hydroxymethyltransferase, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK162421.1 and AK169192.1. Transcript Variant: This variant (2) uses a different splice site in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK169192.1, SRR1660815.214277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGTATCCT TCTCTTTGCT TCGGACCACT CGGAGATGTG GTCAGCTGGT CTGCATGGCT 
GCCCGGGCCC AGCACAGCAA GGTGGCCCAG ACGCAGGCTG GGGAAGCAGC TGGAGGTTGG 
ACGGGCCAGG AGAGTTTATC AGACAGTGAC CCTGAGATGT GGGAGCTTCT GCAGAGGGAG 
AAGGACAGAC AGTGTCGCGG CCTGGAGCTC ATCGCCTCAG AGAACTTCTG CAGCCGAGCT 
GCGCTGGAGG CCCTGGGGTC CTGTCTCAAC AACAAGTACT CGGAGGGTTA CCCTGGCAAG 
AGATACTACG GAGGAGCGGA AGTGGTGGAC GAGATCGAGC TGCTCTGCCA GCGCCGGGCC 
TTGGAAGCCT TTGACCTGGA TCCGGCACAG TGGGGAGTCA ATGTGCAGCC ATACTCAGGG 
TCCCCAGCCA ATCTGGCTGC CTATACGGCC CTTCTGCAGC CTCATGATCG AATCATGGGG 
TTGGACCTGC CCGATGGGGG CCATCTCACC CATGGCTACA TGTCTGATGT CAAGCGGATC 
TCCGCCACAT CCATTTTCTT CGAGTCTATG CCCTATAAGC TCAATCCCCA AACTGGCCTC 
ATCGACTACG ACCAGCTGGC GCTGACCGCT CGGCTTTTCC GACCGCGGCT CATCATAGCT 
GGCACGAGTG CCTATGCCCG CCTCATTGAC TATGCACGCA TGAGAGAGGT CTGTGATGAG 
GTCAGGGCAC ACCTGCTGGC AGACATGGCC CACATCAGTG GCCTGGTGGC TGCCAAGGTG 
ATCCCCTCCC CTTTCAAGTA CGCGGATGTT GTTACCACCA CCACTCACAA GACACTGCGA 
GGGGCCAGGT CAGGGCTCAT CTTCTACCGG AAGGGAGTAC GAACCGTAGA CCCCAAGACT 
GGCAAAGAGA TCCCTTATAC CTTTGAGGAC CGAATCAACT TCGCTGTGTT CCCATCCCTA 
CAGGGTGGCC CCCACAACCA CGCCATTGCT GCAGTAGCCG TGGCTCTCAA GCAGGCCTGC 
ACCCCTATGT TCCGCGAGTA CTCCTTACAA GTGCTGAGGA ACGCCCAGGC CATGGCTGAT 
GCCCTGCTCA AGCGAGGATA CTCGCTGGTG TCTGGTGGCA CTGACACCCA CCTGGTGCTG 
GTGGACCTGC GGCCCAAGGG CCTGGATGGA GCCCGAGCCG AACGCGTGTT GGAACTCGTC 
TCCATCACAG CCAACAAGAA CACCTGTCCT GGAGACCGGA GCGCCATTAC TCCGGGGGGC 
TTGAGGCTTG GGGCCCCCGC GTTGACCTCT CGCCAGTTCC GTGAGGACGA CTTCCGTAGA 
GTCGTCGATT TTATCGATGA GGGAGTCAAC ATTGGCTTGG AGGTGAAGCG CAAGACTGCC 
AAGCTCCAGG ATTTCAAATC CTTCCTGCTC AAAGACCCAG AGACAAGCCA GCGTTTGGCC 
AACCTCCGGC AACAGGTGGA ACAGTTTGCC AGGGGCTTCC CCATGCCTGG ATTTGATGAA 
CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239245.1
CDS	125..1630
Translation	MVSFSLLRTTRRCGQLVCMAARAQHSKVAQTQAGEAAGGWTGQESLSDSDPEMWELLQREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQPYSGSPANLAAYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPQTGLIDYDQLALTARLFRPRLIIAGTSAYARLIDYARMREVCDEVRAHLLADMAHISGLVAAKVIPSPFKYADVVTTTTHKTLRGARSGLIFYRKGVRTVDPKTGKEIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLRNAQAMADALLKRGYSLVSGGTDTHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKRKTAKLQDFKSFLLKDPETSQRLANLRQQVEQFARGFPMPGFDER

Target ORF information:

RefSeq Version	NM_001252316.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252316.1

ORF Insert Sequence:

ATGGTATCCT TCTCTTTGCT TCGGACCACT CGGAGATGTG GTCAGCTGGT CTGCATGGCT 
GCCCGGGCCC AGCACAGCAA GGTGGCCCAG ACGCAGGCTG GGGAAGCAGC TGGAGGTTGG 
ACGGGCCAGG AGAGTTTATC AGACAGTGAC CCTGAGATGT GGGAGCTTCT GCAGAGGGAG 
AAGGACAGAC AGTGTCGCGG CCTGGAGCTC ATCGCCTCAG AGAACTTCTG CAGCCGAGCT 
GCGCTGGAGG CCCTGGGGTC CTGTCTCAAC AACAAGTACT CGGAGGGTTA CCCTGGCAAG 
AGATACTACG GAGGAGCGGA AGTGGTGGAC GAGATCGAGC TGCTCTGCCA GCGCCGGGCC 
TTGGAAGCCT TTGACCTGGA TCCGGCACAG TGGGGAGTCA ATGTGCAGCC ATACTCAGGG 
TCCCCAGCCA ATCTGGCTGC CTATACGGCC CTTCTGCAGC CTCATGATCG AATCATGGGG 
TTGGACCTGC CCGATGGGGG CCATCTCACC CATGGCTACA TGTCTGATGT CAAGCGGATC 
TCCGCCACAT CCATTTTCTT CGAGTCTATG CCCTATAAGC TCAATCCCCA AACTGGCCTC 
ATCGACTACG ACCAGCTGGC GCTGACCGCT CGGCTTTTCC GACCGCGGCT CATCATAGCT 
GGCACGAGTG CCTATGCCCG CCTCATTGAC TATGCACGCA TGAGAGAGGT CTGTGATGAG 
GTCAGGGCAC ACCTGCTGGC AGACATGGCC CACATCAGTG GCCTGGTGGC TGCCAAGGTG 
ATCCCCTCCC CTTTCAAGTA CGCGGATGTT GTTACCACCA CCACTCACAA GACACTGCGA 
GGGGCCAGGT CAGGGCTCAT CTTCTACCGG AAGGGAGTAC GAACCGTAGA CCCCAAGACT 
GGCAAAGAGA TCCCTTATAC CTTTGAGGAC CGAATCAACT TCGCTGTGTT CCCATCCCTA 
CAGGGTGGCC CCCACAACCA CGCCATTGCT GCAGTAGCCG TGGCTCTCAA GCAGGCCTGC 
ACCCCTATGT TCCGCGAGTA CTCCTTACAA GTGCTGAGGA ACGCCCAGGC CATGGCTGAT 
GCCCTGCTCA AGCGAGGATA CTCGCTGGTG TCTGGTGGCA CTGACACCCA CCTGGTGCTG 
GTGGACCTGC GGCCCAAGGG CCTGGATGGA GCCCGAGCCG AACGCGTGTT GGAACTCGTC 
TCCATCACAG CCAACAAGAA CACCTGTCCT GGAGACCGGA GCGCCATTAC TCCGGGGGGC 
TTGAGGCTTG GGGCCCCCGC GTTGACCTCT CGCCAGTTCC GTGAGGACGA CTTCCGTAGA 
GTCGTCGATT TTATCGATGA GGGAGTCAAC ATTGGCTTGG AGGTGAAGCG CAAGACTGCC 
AAGCTCCAGG ATTTCAAATC CTTCCTGCTC AAAGACCCAG AGACAAGCCA GCGTTTGGCC 
AACCTCCGGC AACAGGTGGA ACAGTTTGCC AGGGGCTTCC CCATGCCTGG ATTTGATGAA 
CGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shmt2 ( NM_028230.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082506.1 Mus musculus serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05369
Clone ID Related Accession (Same CDS sequence)	NM_028230.4
Accession Version	NM_028230.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	serine hydroxymethyltransferase, mitochondrial isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK162421.1 and BY760884.1. On Nov 10, 2011 this sequence version replaced NM_028230.3. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AK051207.1, AK162421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGTATCCT TCTCTTTGCT TCGGACCACT CGGCCTCTGC AGAGATGTGG TCAGCTGGTC 
TGCATGGCTG CCCGGGCCCA GCACAGCAAG GTGGCCCAGA CGCAGGCTGG GGAAGCAGCT 
GGAGGTTGGA CGGGCCAGGA GAGTTTATCA GACAGTGACC CTGAGATGTG GGAGCTTCTG 
CAGAGGGAGA AGGACAGACA GTGTCGCGGC CTGGAGCTCA TCGCCTCAGA GAACTTCTGC 
AGCCGAGCTG CGCTGGAGGC CCTGGGGTCC TGTCTCAACA ACAAGTACTC GGAGGGTTAC 
CCTGGCAAGA GATACTACGG AGGAGCGGAA GTGGTGGACG AGATCGAGCT GCTCTGCCAG 
CGCCGGGCCT TGGAAGCCTT TGACCTGGAT CCGGCACAGT GGGGAGTCAA TGTGCAGCCA 
TACTCAGGGT CCCCAGCCAA TCTGGCTGCC TATACGGCCC TTCTGCAGCC TCATGATCGA 
ATCATGGGGT TGGACCTGCC CGATGGGGGC CATCTCACCC ATGGCTACAT GTCTGATGTC 
AAGCGGATCT CCGCCACATC CATTTTCTTC GAGTCTATGC CCTATAAGCT CAATCCCCAA 
ACTGGCCTCA TCGACTACGA CCAGCTGGCG CTGACCGCTC GGCTTTTCCG ACCGCGGCTC 
ATCATAGCTG GCACGAGTGC CTATGCCCGC CTCATTGACT ATGCACGCAT GAGAGAGGTC 
TGTGATGAGG TCAGGGCACA CCTGCTGGCA GACATGGCCC ACATCAGTGG CCTGGTGGCT 
GCCAAGGTGA TCCCCTCCCC TTTCAAGTAC GCGGATGTTG TTACCACCAC CACTCACAAG 
ACACTGCGAG GGGCCAGGTC AGGGCTCATC TTCTACCGGA AGGGAGTACG AACCGTAGAC 
CCCAAGACTG GCAAAGAGAT CCCTTATACC TTTGAGGACC GAATCAACTT CGCTGTGTTC 
CCATCCCTAC AGGGTGGCCC CCACAACCAC GCCATTGCTG CAGTAGCCGT GGCTCTCAAG 
CAGGCCTGCA CCCCTATGTT CCGCGAGTAC TCCTTACAAG TGCTGAGGAA CGCCCAGGCC 
ATGGCTGATG CCCTGCTCAA GCGAGGATAC TCGCTGGTGT CTGGTGGCAC TGACACCCAC 
CTGGTGCTGG TGGACCTGCG GCCCAAGGGC CTGGATGGAG CCCGAGCCGA ACGCGTGTTG 
GAACTCGTCT CCATCACAGC CAACAAGAAC ACCTGTCCTG GAGACCGGAG CGCCATTACT 
CCGGGGGGCT TGAGGCTTGG GGCCCCCGCG TTGACCTCTC GCCAGTTCCG TGAGGACGAC 
TTCCGTAGAG TCGTCGATTT TATCGATGAG GGAGTCAACA TTGGCTTGGA GGTGAAGCGC 
AAGACTGCCA AGCTCCAGGA TTTCAAATCC TTCCTGCTCA AAGACCCAGA GACAAGCCAG 
CGTTTGGCCA ACCTCCGGCA ACAGGTGGAA CAGTTTGCCA GGGGCTTCCC CATGCCTGGA 
TTTGATGAAC GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082506.1
CDS	125..1639
Translation	MVSFSLLRTTRPLQRCGQLVCMAARAQHSKVAQTQAGEAAGGWTGQESLSDSDPEMWELLQREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQPYSGSPANLAAYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPQTGLIDYDQLALTARLFRPRLIIAGTSAYARLIDYARMREVCDEVRAHLLADMAHISGLVAAKVIPSPFKYADVVTTTTHKTLRGARSGLIFYRKGVRTVDPKTGKEIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLRNAQAMADALLKRGYSLVSGGTDTHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKRKTAKLQDFKSFLLKDPETSQRLANLRQQVEQFARGFPMPGFDER

Target ORF information:

RefSeq Version	NM_028230.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine hydroxymethyltransferase 2 (mitochondrial) (Shmt2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028230.4

ORF Insert Sequence:

ATGGTATCCT TCTCTTTGCT TCGGACCACT CGGCCTCTGC AGAGATGTGG TCAGCTGGTC 
TGCATGGCTG CCCGGGCCCA GCACAGCAAG GTGGCCCAGA CGCAGGCTGG GGAAGCAGCT 
GGAGGTTGGA CGGGCCAGGA GAGTTTATCA GACAGTGACC CTGAGATGTG GGAGCTTCTG 
CAGAGGGAGA AGGACAGACA GTGTCGCGGC CTGGAGCTCA TCGCCTCAGA GAACTTCTGC 
AGCCGAGCTG CGCTGGAGGC CCTGGGGTCC TGTCTCAACA ACAAGTACTC GGAGGGTTAC 
CCTGGCAAGA GATACTACGG AGGAGCGGAA GTGGTGGACG AGATCGAGCT GCTCTGCCAG 
CGCCGGGCCT TGGAAGCCTT TGACCTGGAT CCGGCACAGT GGGGAGTCAA TGTGCAGCCA 
TACTCAGGGT CCCCAGCCAA TCTGGCTGCC TATACGGCCC TTCTGCAGCC TCATGATCGA 
ATCATGGGGT TGGACCTGCC CGATGGGGGC CATCTCACCC ATGGCTACAT GTCTGATGTC 
AAGCGGATCT CCGCCACATC CATTTTCTTC GAGTCTATGC CCTATAAGCT CAATCCCCAA 
ACTGGCCTCA TCGACTACGA CCAGCTGGCG CTGACCGCTC GGCTTTTCCG ACCGCGGCTC 
ATCATAGCTG GCACGAGTGC CTATGCCCGC CTCATTGACT ATGCACGCAT GAGAGAGGTC 
TGTGATGAGG TCAGGGCACA CCTGCTGGCA GACATGGCCC ACATCAGTGG CCTGGTGGCT 
GCCAAGGTGA TCCCCTCCCC TTTCAAGTAC GCGGATGTTG TTACCACCAC CACTCACAAG 
ACACTGCGAG GGGCCAGGTC AGGGCTCATC TTCTACCGGA AGGGAGTACG AACCGTAGAC 
CCCAAGACTG GCAAAGAGAT CCCTTATACC TTTGAGGACC GAATCAACTT CGCTGTGTTC 
CCATCCCTAC AGGGTGGCCC CCACAACCAC GCCATTGCTG CAGTAGCCGT GGCTCTCAAG 
CAGGCCTGCA CCCCTATGTT CCGCGAGTAC TCCTTACAAG TGCTGAGGAA CGCCCAGGCC 
ATGGCTGATG CCCTGCTCAA GCGAGGATAC TCGCTGGTGT CTGGTGGCAC TGACACCCAC 
CTGGTGCTGG TGGACCTGCG GCCCAAGGGC CTGGATGGAG CCCGAGCCGA ACGCGTGTTG 
GAACTCGTCT CCATCACAGC CAACAAGAAC ACCTGTCCTG GAGACCGGAG CGCCATTACT 
CCGGGGGGCT TGAGGCTTGG GGCCCCCGCG TTGACCTCTC GCCAGTTCCG TGAGGACGAC 
TTCCGTAGAG TCGTCGATTT TATCGATGAG GGAGTCAACA TTGGCTTGGA GGTGAAGCGC 
AAGACTGCCA AGCTCCAGGA TTTCAAATCC TTCCTGCTCA AAGACCCAGA GACAAGCCAG 
CGTTTGGCCA ACCTCCGGCA ACAGGTGGAA CAGTTTGCCA GGGGCTTCCC CATGCCTGGA 
TTTGATGAAC GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mice deficient in the Shmt2 gene have mitochondrial respiration defects and are embryonic lethal.
Scientific reports8(1)425(2018 Jan)
Tani H,Ohnishi S,Shitara H,Mito T,Yamaguchi M,Yonekawa H,Hashizume O,Ishikawa K,Nakada K,Hayashi JI

Karyopherin Alpha 1 Regulates Satellite Cell Proliferation and Survival by Modulating Nuclear Import.
Stem cells (Dayton, Ohio)34(11)2784-2797(2016 Nov)
Choo HJ,Cutler A,Rother F,Bader M,Pavlath GK

SHMT1 and SHMT2 are functionally redundant in nuclear de novo thymidylate biosynthesis.
PloS one4(6)e5839(2009 Jun)
Anderson DD,Stover PJ

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Shmt2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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