Chrnb4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrnb4
Entrez Gene ID	108015
Full Name	cholinergic receptor, nicotinic, beta polypeptide 4
Synonyms	Acrb-4,Acrb4
General protein information	Preferred Names cholinergic receptor, nicotinic, beta polypeptide 4 Names neuronal acetylcholine receptor subunit beta-4 nicotinic acetylcholine receptor beta4 subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 B

mRNA and Protein(s)

mRNA	Protein	Name
XM_011242655.2	XP_011240957.1	neuronal acetylcholine receptor subunit beta-4 isoform X1
XM_006510757.3	XP_006510820.1	neuronal acetylcholine receptor subunit beta-4 isoform X1
XM_011242655.3	XP_011240957.1	neuronal acetylcholine receptor subunit beta-4 isoform X1
XM_006510757.4	XP_006510820.1	neuronal acetylcholine receptor subunit beta-4 isoform X1
NM_148944.4	NP_683746.1	neuronal acetylcholine receptor subunit beta-4 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CHRNB4	NP_990150.1
Homo sapiens (human)	CHRNB4	NP_000741.1
Bos taurus (cattle)	CHRNB4	NP_776942.1
Xenopus tropicalis (tropical clawed frog)	chrnb4	XP_002934622.2
Pan troglodytes (chimpanzee)	CHRNB4	XP_510529.3
Canis lupus familiaris (dog)	CHRNB4	XP_852548.2
Rattus norvegicus (Norway rat)	Chrnb4	NP_434693.1
Danio rerio (zebrafish)	LOC568566	XP_696993.2
	LOC716649	XP_001107489.2
Mus musculus (house mouse)	Chrnb4	NP_683746.1

Gene Ontology
Bibliography

Related articles in PubMed

No evidence of a role of the ?4 subunit of the nicotinic acetylcholine receptor in alcohol-related behaviors.
Kamens HM, Silva C, McCarthy R, Cox RJ, Ehringer MA
BMC research notes10(1)151(2017 Apr)

Role of ?4* Nicotinic Acetylcholine Receptors in the Habenulo-Interpeduncular Pathway in Nicotine Reinforcement in Mice.
Harrington L, Vi?als X, Herrera-Sol?s A, Flores A, Morel C, Tolu S, Faure P, Maldonado R, Maskos U, Robledo P
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology41(7)1790-802(2016 06)

MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes.
Ganic E, Singh T, Luan C, Fadista J, Johansson JK, Cyphert HA, Bennet H, Storm P, Prost G, Ahlenius H, Renstr?m E, Stein R, Groop L, Fex M, Artner I
Cell reports14(8)1991-2002(2016 Mar)

Changes in Nicotinic Neurotransmission during Enteric Nervous System Development.
Foong JP, Hirst CS, Hao MM, McKeown SJ, Boesmans W, Young HM, Bornstein JC, Vanden Berghe P
The Journal of neuroscience : the official journal of the Society for Neuroscience35(18)7106-15(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that the nicotinic acetylcholine receptor beta4 subunit (beta4 subunit) is not involved in ethanol responses.
Title: No evidence of a role of the ?4 subunit of the nicotinic acetylcholine receptor in alcohol-related behaviors.

These findings suggest that beta4* nAChRs in the interpeduncular nucleus have a role in maintaining nicotine intravenous self-administration.
Title: Role of ?4* Nicotinic Acetylcholine Receptors in the Habenulo-Interpeduncular Pathway in Nicotine Reinforcement in Mice.

MAFA controls autonomic nervous system-mediated insulin secretion by activating the transcription of nicotinic (ChrnB2 and ChrnB4) receptor genes, which is impaired in type 2 diabetes.
Title: MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes.

The alpha3beta4* nicotinic ACh receptor subtype mediates physical dependence to morphine
Title: The ?3?4* nicotinic ACh receptor subtype mediates physical dependence to morphine: mouse and human studies.

Downregulation of nAChR subunit and PSD-93 expression after cavernous nerve injury, or even manipulation, could interrupt synaptic transmission within the MPG and thus contribute to the loss of neural control of urogenital organs after pelvic surgeries.
Title: Decrease in neuronal nicotinic acetylcholine receptor subunit and PSD-93 transcript levels in the male mouse MPG after cavernous nerve injury or explant culture.

The following Chrnb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrnb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02173	NM_148944.4 Latest version!	Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu42502	XM_011242655.3 Latest version!	Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu42502	XM_006510757.4 Latest version!	Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu42502	XM_011242655.2	Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu42502	XM_006510757.3	Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrnb4 ( NM_148944.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_683746.1 Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), mRNA.

Price & Availability↑

CloneID	OMu02173
Clone ID Related Accession (Same CDS sequence)	NM_148944.4
Accession Version	NM_148944.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	neuronal acetylcholine receptor subunit beta-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC156832.2. On May 1, 2007 this sequence version replaced NM_148944.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK140071.1, BC138851.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGAGGGGTA CGCCCCTGCT CCTCGTCTCT CTGTTCGCTC TGCTTCAGCC TGGGGACTGC 
CGCCTGGCCA ACGCAGAGGA GAAGCTGATG GATGATCTCC TGAACAAAAC CCGCTACAAC 
AACCTGATCC GCCCAGCCAC CAGCTCCTCC CAGCTCATCT CCATCCGCCT GGAGCTATCA 
CTGTCCCAGC TCATCAGTGT GAATGAGCGA GAACAGATCA TGACCACCAG CATCTGGCTG 
AAACAGGAAT GGACTGACTA CCGCCTGGCC TGGAACAGCT CCTGCTATGA AGGGGTGAAC 
ATTCTGAGGA TTCCTGCAAA GCGAGTCTGG TTGCCTGACA TCGTGTTGTA CAACAATGCC 
GATGGGACCT ATGAGGTGTC TGTCTACACC AACGTGATCG TGCGTTCCAA CGGCAGCATC 
CAGTGGCTGC CCCCTGCCAT CTACAAGAGT GCCTGCAAGA TAGAGGTGAA GCACTTTCCC 
TTCGACCAGC AGAACTGCAC CCTCAAATTT CGCTCCTGGA CCTATGACCA CACAGAGATT 
GACATGGTTC TTAAATCACC CACAGCCATC ATGGATGACT TTACCCCCAG CGGTGAATGG 
GACATTGTGG CCCTCCCGGG TCGAAGGACA GTGAACCCAC AGGACCCCAG CTATGTGGAC 
GTGACCTATG ACTTCATCAT CAAGCGCAAG CCACTCTTCT ACACCATCAA TCTGATCATC 
CCTTGTGTGC TCATCACGTC GCTGGCTATC CTGGTCTTCT ACCTGCCCTC CGACTGTGGG 
GAGAAGATGA CGCTCTGCAT CTCAGTGCTG CTGGCACTCA CATTCTTCCT GCTGCTCATC 
TCCAAGATCG TGCCTCCCAC CTCCCTTGAC ATTCCCCTCA TTGGCAAGTA CCTCTTGTTC 
ACCATGGTGC TGGTCACCTT TTCCATCGTC ACCACTGTGT GTGTCCTCAA TGTGCACCAC 
CGTTCACCCA GCACTCACAC CATGGCATCC TGGGTCAAGG AGTGCTTCCT GCACAAGCTG 
CCCACCTTCC TCTTCATGAA GCGCCCTGGT CTGGAAGTCA GCCCCGCCAG GGTCCCTCAT 
TCCAGCCAGC TGCACTTGAC CACAGCTGAA GCTACGTCCA CCTCTGCCTT AGGCCCCAGC 
AGCCCATCCA ACCTCTATGG GAATTCCATG TACTTTGTGA ACCCTGTCCC TGCCACTCCT 
AAGTCTGCAG TCAGCTCCCA CACGGCAGGC CTCCCCAGGG ATGCCCGGCT GAGGTCCTCT 
GGGAGGTTCC GGCAAGATCT ACAGGAAGCA TTAGAGGGCG TCAGCTTCAT CGCACAGCAT 
TTGGAGAGTG ATGATCGAGA TCAGAGTGTC ATCGAGGACT GGAAATTCGT CGCAATGGTC 
GTCGACCGCC TGTTCCTGTG GGTGTTCGTG ATTGTGTGTA TTCTGGGCAC CATGGGGCTC 
TTCCTGCCAC CCCTCTTCCA GATCCACGCA CCCTCCAAGG GCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683746.1
CDS	50..1537
Translation	MRGTPLLLVSLFALLQPGDCRLANAEEKLMDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAWNSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKECFLHKLPTFLFMKRPGLEVSPARVPHSSQLHLTTAEATSTSALGPSSPSNLYGNSMYFVNPVPATPKSAVSSHTAGLPRDARLRSSGRFRQDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAMVVDRLFLWVFVIVCILGTMGLFLPPLFQIHAPSKGL

Target ORF information:

RefSeq Version	NM_148944.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148944.4

ORF Insert Sequence:

ATGAGGGGTA CGCCCCTGCT CCTCGTCTCT CTGTTCGCTC TGCTTCAGCC TGGGGACTGC 
CGCCTGGCCA ACGCAGAGGA GAAGCTGATG GATGATCTCC TGAACAAAAC CCGCTACAAC 
AACCTGATCC GCCCAGCCAC CAGCTCCTCC CAGCTCATCT CCATCCGCCT GGAGCTATCA 
CTGTCCCAGC TCATCAGTGT GAATGAGCGA GAACAGATCA TGACCACCAG CATCTGGCTG 
AAACAGGAAT GGACTGACTA CCGCCTGGCC TGGAACAGCT CCTGCTATGA AGGGGTGAAC 
ATTCTGAGGA TTCCTGCAAA GCGAGTCTGG TTGCCTGACA TCGTGTTGTA CAACAATGCC 
GATGGGACCT ATGAGGTGTC TGTCTACACC AACGTGATCG TGCGTTCCAA CGGCAGCATC 
CAGTGGCTGC CCCCTGCCAT CTACAAGAGT GCCTGCAAGA TAGAGGTGAA GCACTTTCCC 
TTCGACCAGC AGAACTGCAC CCTCAAATTT CGCTCCTGGA CCTATGACCA CACAGAGATT 
GACATGGTTC TTAAATCACC CACAGCCATC ATGGATGACT TTACCCCCAG CGGTGAATGG 
GACATTGTGG CCCTCCCGGG TCGAAGGACA GTGAACCCAC AGGACCCCAG CTATGTGGAC 
GTGACCTATG ACTTCATCAT CAAGCGCAAG CCACTCTTCT ACACCATCAA TCTGATCATC 
CCTTGTGTGC TCATCACGTC GCTGGCTATC CTGGTCTTCT ACCTGCCCTC CGACTGTGGG 
GAGAAGATGA CGCTCTGCAT CTCAGTGCTG CTGGCACTCA CATTCTTCCT GCTGCTCATC 
TCCAAGATCG TGCCTCCCAC CTCCCTTGAC ATTCCCCTCA TTGGCAAGTA CCTCTTGTTC 
ACCATGGTGC TGGTCACCTT TTCCATCGTC ACCACTGTGT GTGTCCTCAA TGTGCACCAC 
CGTTCACCCA GCACTCACAC CATGGCATCC TGGGTCAAGG AGTGCTTCCT GCACAAGCTG 
CCCACCTTCC TCTTCATGAA GCGCCCTGGT CTGGAAGTCA GCCCCGCCAG GGTCCCTCAT 
TCCAGCCAGC TGCACTTGAC CACAGCTGAA GCTACGTCCA CCTCTGCCTT AGGCCCCAGC 
AGCCCATCCA ACCTCTATGG GAATTCCATG TACTTTGTGA ACCCTGTCCC TGCCACTCCT 
AAGTCTGCAG TCAGCTCCCA CACGGCAGGC CTCCCCAGGG ATGCCCGGCT GAGGTCCTCT 
GGGAGGTTCC GGCAAGATCT ACAGGAAGCA TTAGAGGGCG TCAGCTTCAT CGCACAGCAT 
TTGGAGAGTG ATGATCGAGA TCAGAGTGTC ATCGAGGACT GGAAATTCGT CGCAATGGTC 
GTCGACCGCC TGTTCCTGTG GGTGTTCGTG ATTGTGTGTA TTCTGGGCAC CATGGGGCTC 
TTCCTGCCAC CCCTCTTCCA GATCCACGCA CCCTCCAAGG GCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrnb4 ( XM_011242655.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240957.1 Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42502
Clone ID Related Accession (Same CDS sequence)	XM_011242655.2 , XM_006510757.3 , XM_011242655.3 , XM_006510757.4
Accession Version	XM_011242655.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuronal acetylcholine receptor subunit beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATC TCCTGAACAA AACCCGCTAC AACAACCTGA TCCGCCCAGC CACCAGCTCC 
TCCCAGCTCA TCTCCATCCG CCTGGAGCTA TCACTGTCCC AGCTCATCAG TGTGAATGAG 
CGAGAACAGA TCATGACCAC CAGCATCTGG CTGAAACAGG AATGGACTGA CTACCGCCTG 
GCCTGGAACA GCTCCTGCTA TGAAGGGGTG AACATTCTGA GGATTCCTGC AAAGCGAGTC 
TGGTTGCCTG ACATCGTGTT GTACAACAAT GCCGATGGGA CCTATGAGGT GTCTGTCTAC 
ACCAACGTGA TCGTGCGTTC CAACGGCAGC ATCCAGTGGC TGCCCCCTGC CATCTACAAG 
AGTGCCTGCA AGATAGAGGT GAAGCACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAA 
TTTCGCTCCT GGACCTATGA CCACACAGAG ATTGACATGG TTCTTAAATC ACCCACAGCC 
ATCATGGATG ACTTTACCCC CAGCGGTGAA TGGGACATTG TGGCCCTCCC GGGTCGAAGG 
ACAGTGAACC CACAGGACCC CAGCTATGTG GACGTGACCT ATGACTTCAT CATCAAGCGC 
AAGCCACTCT TCTACACCAT CAATCTGATC ATCCCTTGTG TGCTCATCAC GTCGCTGGCT 
ATCCTGGTCT TCTACCTGCC CTCCGACTGT GGGGAGAAGA TGACGCTCTG CATCTCAGTG 
CTGCTGGCAC TCACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCTCC CACCTCCCTT 
GACATTCCCC TCATTGGCAA GTACCTCTTG TTCACCATGG TGCTGGTCAC CTTTTCCATC 
GTCACCACTG TGTGTGTCCT CAATGTGCAC CACCGTTCAC CCAGCACTCA CACCATGGCA 
TCCTGGGTCA AGGAGTGCTT CCTGCACAAG CTGCCCACCT TCCTCTTCAT GAAGCGCCCT 
GGTCTGGAAG TCAGCCCCGC CAGGGTCCCT CATTCCAGCC AGCTGCACTT GACCACAGCT 
GAAGCTACGT CCACCTCTGC CTTAGGCCCC AGCAGCCCAT CCAACCTCTA TGGGAATTCC 
ATGTACTTTG TGAACCCTGT CCCTGCCACT CCTAAGTCTG CAGTCAGCTC CCACACGGCA 
GGCCTCCCCA GGGATGCCCG GCTGAGGTCC TCTGGGAGGT TCCGGCAAGA TCTACAGGAA 
GCATTAGAGG GCGTCAGCTT CATCGCACAG CATTTGGAGA GTGATGATCG AGATCAGAGT 
GTCATCGAGG ACTGGAAATT CGTCGCAATG GTCGTCGACC GCCTGTTCCT GTGGGTGTTC 
GTGATTGTGT GTATTCTGGG CACCATGGGG CTCTTCCTGC CACCCCTCTT CCAGATCCAC 
GCACCCTCCA AGGGCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240957.1
CDS	603..2003
Translation	MDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAWNSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKECFLHKLPTFLFMKRPGLEVSPARVPHSSQLHLTTAEATSTSALGPSSPSNLYGNSMYFVNPVPATPKSAVSSHTAGLPRDARLRSSGRFRQDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAMVVDRLFLWVFVIVCILGTMGLFLPPLFQIHAPSKGL

Target ORF information:

RefSeq Version	XM_011242655.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242655.2

ORF Insert Sequence:

ATGGATGATC TCCTGAACAA AACCCGCTAC AACAACCTGA TCCGCCCAGC CACCAGCTCC 
TCCCAGCTCA TCTCCATCCG CCTGGAGCTA TCACTGTCCC AGCTCATCAG TGTGAATGAG 
CGAGAACAGA TCATGACCAC CAGCATCTGG CTGAAACAGG AATGGACTGA CTACCGCCTG 
GCCTGGAACA GCTCCTGCTA TGAAGGGGTG AACATTCTGA GGATTCCTGC AAAGCGAGTC 
TGGTTGCCTG ACATCGTGTT GTACAACAAT GCCGATGGGA CCTATGAGGT GTCTGTCTAC 
ACCAACGTGA TCGTGCGTTC CAACGGCAGC ATCCAGTGGC TGCCCCCTGC CATCTACAAG 
AGTGCCTGCA AGATAGAGGT GAAGCACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAA 
TTTCGCTCCT GGACCTATGA CCACACAGAG ATTGACATGG TTCTTAAATC ACCCACAGCC 
ATCATGGATG ACTTTACCCC CAGCGGTGAA TGGGACATTG TGGCCCTCCC GGGTCGAAGG 
ACAGTGAACC CACAGGACCC CAGCTATGTG GACGTGACCT ATGACTTCAT CATCAAGCGC 
AAGCCACTCT TCTACACCAT CAATCTGATC ATCCCTTGTG TGCTCATCAC GTCGCTGGCT 
ATCCTGGTCT TCTACCTGCC CTCCGACTGT GGGGAGAAGA TGACGCTCTG CATCTCAGTG 
CTGCTGGCAC TCACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCTCC CACCTCCCTT 
GACATTCCCC TCATTGGCAA GTACCTCTTG TTCACCATGG TGCTGGTCAC CTTTTCCATC 
GTCACCACTG TGTGTGTCCT CAATGTGCAC CACCGTTCAC CCAGCACTCA CACCATGGCA 
TCCTGGGTCA AGGAGTGCTT CCTGCACAAG CTGCCCACCT TCCTCTTCAT GAAGCGCCCT 
GGTCTGGAAG TCAGCCCCGC CAGGGTCCCT CATTCCAGCC AGCTGCACTT GACCACAGCT 
GAAGCTACGT CCACCTCTGC CTTAGGCCCC AGCAGCCCAT CCAACCTCTA TGGGAATTCC 
ATGTACTTTG TGAACCCTGT CCCTGCCACT CCTAAGTCTG CAGTCAGCTC CCACACGGCA 
GGCCTCCCCA GGGATGCCCG GCTGAGGTCC TCTGGGAGGT TCCGGCAAGA TCTACAGGAA 
GCATTAGAGG GCGTCAGCTT CATCGCACAG CATTTGGAGA GTGATGATCG AGATCAGAGT 
GTCATCGAGG ACTGGAAATT CGTCGCAATG GTCGTCGACC GCCTGTTCCT GTGGGTGTTC 
GTGATTGTGT GTATTCTGGG CACCATGGGG CTCTTCCTGC CACCCCTCTT CCAGATCCAC 
GCACCCTCCA AGGGCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrnb4 ( XM_006510757.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510820.1 Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu42502
Clone ID Related Accession (Same CDS sequence)	XM_011242655.2 , XM_006510757.3 , XM_011242655.3 , XM_006510757.4
Accession Version	XM_006510757.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neuronal acetylcholine receptor subunit beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510757.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATC TCCTGAACAA AACCCGCTAC AACAACCTGA TCCGCCCAGC CACCAGCTCC 
TCCCAGCTCA TCTCCATCCG CCTGGAGCTA TCACTGTCCC AGCTCATCAG TGTGAATGAG 
CGAGAACAGA TCATGACCAC CAGCATCTGG CTGAAACAGG AATGGACTGA CTACCGCCTG 
GCCTGGAACA GCTCCTGCTA TGAAGGGGTG AACATTCTGA GGATTCCTGC AAAGCGAGTC 
TGGTTGCCTG ACATCGTGTT GTACAACAAT GCCGATGGGA CCTATGAGGT GTCTGTCTAC 
ACCAACGTGA TCGTGCGTTC CAACGGCAGC ATCCAGTGGC TGCCCCCTGC CATCTACAAG 
AGTGCCTGCA AGATAGAGGT GAAGCACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAA 
TTTCGCTCCT GGACCTATGA CCACACAGAG ATTGACATGG TTCTTAAATC ACCCACAGCC 
ATCATGGATG ACTTTACCCC CAGCGGTGAA TGGGACATTG TGGCCCTCCC GGGTCGAAGG 
ACAGTGAACC CACAGGACCC CAGCTATGTG GACGTGACCT ATGACTTCAT CATCAAGCGC 
AAGCCACTCT TCTACACCAT CAATCTGATC ATCCCTTGTG TGCTCATCAC GTCGCTGGCT 
ATCCTGGTCT TCTACCTGCC CTCCGACTGT GGGGAGAAGA TGACGCTCTG CATCTCAGTG 
CTGCTGGCAC TCACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCTCC CACCTCCCTT 
GACATTCCCC TCATTGGCAA GTACCTCTTG TTCACCATGG TGCTGGTCAC CTTTTCCATC 
GTCACCACTG TGTGTGTCCT CAATGTGCAC CACCGTTCAC CCAGCACTCA CACCATGGCA 
TCCTGGGTCA AGGAGTGCTT CCTGCACAAG CTGCCCACCT TCCTCTTCAT GAAGCGCCCT 
GGTCTGGAAG TCAGCCCCGC CAGGGTCCCT CATTCCAGCC AGCTGCACTT GACCACAGCT 
GAAGCTACGT CCACCTCTGC CTTAGGCCCC AGCAGCCCAT CCAACCTCTA TGGGAATTCC 
ATGTACTTTG TGAACCCTGT CCCTGCCACT CCTAAGTCTG CAGTCAGCTC CCACACGGCA 
GGCCTCCCCA GGGATGCCCG GCTGAGGTCC TCTGGGAGGT TCCGGCAAGA TCTACAGGAA 
GCATTAGAGG GCGTCAGCTT CATCGCACAG CATTTGGAGA GTGATGATCG AGATCAGAGT 
GTCATCGAGG ACTGGAAATT CGTCGCAATG GTCGTCGACC GCCTGTTCCT GTGGGTGTTC 
GTGATTGTGT GTATTCTGGG CACCATGGGG CTCTTCCTGC CACCCCTCTT CCAGATCCAC 
GCACCCTCCA AGGGCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510820.1
CDS	194..1594
Translation	MDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAWNSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKECFLHKLPTFLFMKRPGLEVSPARVPHSSQLHLTTAEATSTSALGPSSPSNLYGNSMYFVNPVPATPKSAVSSHTAGLPRDARLRSSGRFRQDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAMVVDRLFLWVFVIVCILGTMGLFLPPLFQIHAPSKGL

Target ORF information:

RefSeq Version	XM_006510757.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510757.3

ORF Insert Sequence:

ATGGATGATC TCCTGAACAA AACCCGCTAC AACAACCTGA TCCGCCCAGC CACCAGCTCC 
TCCCAGCTCA TCTCCATCCG CCTGGAGCTA TCACTGTCCC AGCTCATCAG TGTGAATGAG 
CGAGAACAGA TCATGACCAC CAGCATCTGG CTGAAACAGG AATGGACTGA CTACCGCCTG 
GCCTGGAACA GCTCCTGCTA TGAAGGGGTG AACATTCTGA GGATTCCTGC AAAGCGAGTC 
TGGTTGCCTG ACATCGTGTT GTACAACAAT GCCGATGGGA CCTATGAGGT GTCTGTCTAC 
ACCAACGTGA TCGTGCGTTC CAACGGCAGC ATCCAGTGGC TGCCCCCTGC CATCTACAAG 
AGTGCCTGCA AGATAGAGGT GAAGCACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAA 
TTTCGCTCCT GGACCTATGA CCACACAGAG ATTGACATGG TTCTTAAATC ACCCACAGCC 
ATCATGGATG ACTTTACCCC CAGCGGTGAA TGGGACATTG TGGCCCTCCC GGGTCGAAGG 
ACAGTGAACC CACAGGACCC CAGCTATGTG GACGTGACCT ATGACTTCAT CATCAAGCGC 
AAGCCACTCT TCTACACCAT CAATCTGATC ATCCCTTGTG TGCTCATCAC GTCGCTGGCT 
ATCCTGGTCT TCTACCTGCC CTCCGACTGT GGGGAGAAGA TGACGCTCTG CATCTCAGTG 
CTGCTGGCAC TCACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCTCC CACCTCCCTT 
GACATTCCCC TCATTGGCAA GTACCTCTTG TTCACCATGG TGCTGGTCAC CTTTTCCATC 
GTCACCACTG TGTGTGTCCT CAATGTGCAC CACCGTTCAC CCAGCACTCA CACCATGGCA 
TCCTGGGTCA AGGAGTGCTT CCTGCACAAG CTGCCCACCT TCCTCTTCAT GAAGCGCCCT 
GGTCTGGAAG TCAGCCCCGC CAGGGTCCCT CATTCCAGCC AGCTGCACTT GACCACAGCT 
GAAGCTACGT CCACCTCTGC CTTAGGCCCC AGCAGCCCAT CCAACCTCTA TGGGAATTCC 
ATGTACTTTG TGAACCCTGT CCCTGCCACT CCTAAGTCTG CAGTCAGCTC CCACACGGCA 
GGCCTCCCCA GGGATGCCCG GCTGAGGTCC TCTGGGAGGT TCCGGCAAGA TCTACAGGAA 
GCATTAGAGG GCGTCAGCTT CATCGCACAG CATTTGGAGA GTGATGATCG AGATCAGAGT 
GTCATCGAGG ACTGGAAATT CGTCGCAATG GTCGTCGACC GCCTGTTCCT GTGGGTGTTC 
GTGATTGTGT GTATTCTGGG CACCATGGGG CTCTTCCTGC CACCCCTCTT CCAGATCCAC 
GCACCCTCCA AGGGCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrnb4 ( XM_011242655.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240957.1 Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X1, mRNA.

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CloneID	OMu42502
Clone ID Related Accession (Same CDS sequence)	XM_011242655.2 , XM_006510757.3 , XM_011242655.3 , XM_006510757.4
Accession Version	XM_011242655.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuronal acetylcholine receptor subunit beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242655.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATC TCCTGAACAA AACCCGCTAC AACAACCTGA TCCGCCCAGC CACCAGCTCC 
TCCCAGCTCA TCTCCATCCG CCTGGAGCTA TCACTGTCCC AGCTCATCAG TGTGAATGAG 
CGAGAACAGA TCATGACCAC CAGCATCTGG CTGAAACAGG AATGGACTGA CTACCGCCTG 
GCCTGGAACA GCTCCTGCTA TGAAGGGGTG AACATTCTGA GGATTCCTGC AAAGCGAGTC 
TGGTTGCCTG ACATCGTGTT GTACAACAAT GCCGATGGGA CCTATGAGGT GTCTGTCTAC 
ACCAACGTGA TCGTGCGTTC CAACGGCAGC ATCCAGTGGC TGCCCCCTGC CATCTACAAG 
AGTGCCTGCA AGATAGAGGT GAAGCACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAA 
TTTCGCTCCT GGACCTATGA CCACACAGAG ATTGACATGG TTCTTAAATC ACCCACAGCC 
ATCATGGATG ACTTTACCCC CAGCGGTGAA TGGGACATTG TGGCCCTCCC GGGTCGAAGG 
ACAGTGAACC CACAGGACCC CAGCTATGTG GACGTGACCT ATGACTTCAT CATCAAGCGC 
AAGCCACTCT TCTACACCAT CAATCTGATC ATCCCTTGTG TGCTCATCAC GTCGCTGGCT 
ATCCTGGTCT TCTACCTGCC CTCCGACTGT GGGGAGAAGA TGACGCTCTG CATCTCAGTG 
CTGCTGGCAC TCACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCTCC CACCTCCCTT 
GACATTCCCC TCATTGGCAA GTACCTCTTG TTCACCATGG TGCTGGTCAC CTTTTCCATC 
GTCACCACTG TGTGTGTCCT CAATGTGCAC CACCGTTCAC CCAGCACTCA CACCATGGCA 
TCCTGGGTCA AGGAGTGCTT CCTGCACAAG CTGCCCACCT TCCTCTTCAT GAAGCGCCCT 
GGTCTGGAAG TCAGCCCCGC CAGGGTCCCT CATTCCAGCC AGCTGCACTT GACCACAGCT 
GAAGCTACGT CCACCTCTGC CTTAGGCCCC AGCAGCCCAT CCAACCTCTA TGGGAATTCC 
ATGTACTTTG TGAACCCTGT CCCTGCCACT CCTAAGTCTG CAGTCAGCTC CCACACGGCA 
GGCCTCCCCA GGGATGCCCG GCTGAGGTCC TCTGGGAGGT TCCGGCAAGA TCTACAGGAA 
GCATTAGAGG GCGTCAGCTT CATCGCACAG CATTTGGAGA GTGATGATCG AGATCAGAGT 
GTCATCGAGG ACTGGAAATT CGTCGCAATG GTCGTCGACC GCCTGTTCCT GTGGGTGTTC 
GTGATTGTGT GTATTCTGGG CACCATGGGG CTCTTCCTGC CACCCCTCTT CCAGATCCAC 
GCACCCTCCA AGGGCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240957.1
CDS	603..2003
Translation	MDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAWNSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKECFLHKLPTFLFMKRPGLEVSPARVPHSSQLHLTTAEATSTSALGPSSPSNLYGNSMYFVNPVPATPKSAVSSHTAGLPRDARLRSSGRFRQDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAMVVDRLFLWVFVIVCILGTMGLFLPPLFQIHAPSKGL

Target ORF information:

RefSeq Version	XM_011242655.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242655.3

ORF Insert Sequence:

ATGGATGATC TCCTGAACAA AACCCGCTAC AACAACCTGA TCCGCCCAGC CACCAGCTCC 
TCCCAGCTCA TCTCCATCCG CCTGGAGCTA TCACTGTCCC AGCTCATCAG TGTGAATGAG 
CGAGAACAGA TCATGACCAC CAGCATCTGG CTGAAACAGG AATGGACTGA CTACCGCCTG 
GCCTGGAACA GCTCCTGCTA TGAAGGGGTG AACATTCTGA GGATTCCTGC AAAGCGAGTC 
TGGTTGCCTG ACATCGTGTT GTACAACAAT GCCGATGGGA CCTATGAGGT GTCTGTCTAC 
ACCAACGTGA TCGTGCGTTC CAACGGCAGC ATCCAGTGGC TGCCCCCTGC CATCTACAAG 
AGTGCCTGCA AGATAGAGGT GAAGCACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAA 
TTTCGCTCCT GGACCTATGA CCACACAGAG ATTGACATGG TTCTTAAATC ACCCACAGCC 
ATCATGGATG ACTTTACCCC CAGCGGTGAA TGGGACATTG TGGCCCTCCC GGGTCGAAGG 
ACAGTGAACC CACAGGACCC CAGCTATGTG GACGTGACCT ATGACTTCAT CATCAAGCGC 
AAGCCACTCT TCTACACCAT CAATCTGATC ATCCCTTGTG TGCTCATCAC GTCGCTGGCT 
ATCCTGGTCT TCTACCTGCC CTCCGACTGT GGGGAGAAGA TGACGCTCTG CATCTCAGTG 
CTGCTGGCAC TCACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCTCC CACCTCCCTT 
GACATTCCCC TCATTGGCAA GTACCTCTTG TTCACCATGG TGCTGGTCAC CTTTTCCATC 
GTCACCACTG TGTGTGTCCT CAATGTGCAC CACCGTTCAC CCAGCACTCA CACCATGGCA 
TCCTGGGTCA AGGAGTGCTT CCTGCACAAG CTGCCCACCT TCCTCTTCAT GAAGCGCCCT 
GGTCTGGAAG TCAGCCCCGC CAGGGTCCCT CATTCCAGCC AGCTGCACTT GACCACAGCT 
GAAGCTACGT CCACCTCTGC CTTAGGCCCC AGCAGCCCAT CCAACCTCTA TGGGAATTCC 
ATGTACTTTG TGAACCCTGT CCCTGCCACT CCTAAGTCTG CAGTCAGCTC CCACACGGCA 
GGCCTCCCCA GGGATGCCCG GCTGAGGTCC TCTGGGAGGT TCCGGCAAGA TCTACAGGAA 
GCATTAGAGG GCGTCAGCTT CATCGCACAG CATTTGGAGA GTGATGATCG AGATCAGAGT 
GTCATCGAGG ACTGGAAATT CGTCGCAATG GTCGTCGACC GCCTGTTCCT GTGGGTGTTC 
GTGATTGTGT GTATTCTGGG CACCATGGGG CTCTTCCTGC CACCCCTCTT CCAGATCCAC 
GCACCCTCCA AGGGCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrnb4 ( XM_006510757.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510820.1 Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X2, mRNA.

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CloneID	OMu42502
Clone ID Related Accession (Same CDS sequence)	XM_011242655.2 , XM_006510757.3 , XM_011242655.3 , XM_006510757.4
Accession Version	XM_006510757.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neuronal acetylcholine receptor subunit beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510757.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATC TCCTGAACAA AACCCGCTAC AACAACCTGA TCCGCCCAGC CACCAGCTCC 
TCCCAGCTCA TCTCCATCCG CCTGGAGCTA TCACTGTCCC AGCTCATCAG TGTGAATGAG 
CGAGAACAGA TCATGACCAC CAGCATCTGG CTGAAACAGG AATGGACTGA CTACCGCCTG 
GCCTGGAACA GCTCCTGCTA TGAAGGGGTG AACATTCTGA GGATTCCTGC AAAGCGAGTC 
TGGTTGCCTG ACATCGTGTT GTACAACAAT GCCGATGGGA CCTATGAGGT GTCTGTCTAC 
ACCAACGTGA TCGTGCGTTC CAACGGCAGC ATCCAGTGGC TGCCCCCTGC CATCTACAAG 
AGTGCCTGCA AGATAGAGGT GAAGCACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAA 
TTTCGCTCCT GGACCTATGA CCACACAGAG ATTGACATGG TTCTTAAATC ACCCACAGCC 
ATCATGGATG ACTTTACCCC CAGCGGTGAA TGGGACATTG TGGCCCTCCC GGGTCGAAGG 
ACAGTGAACC CACAGGACCC CAGCTATGTG GACGTGACCT ATGACTTCAT CATCAAGCGC 
AAGCCACTCT TCTACACCAT CAATCTGATC ATCCCTTGTG TGCTCATCAC GTCGCTGGCT 
ATCCTGGTCT TCTACCTGCC CTCCGACTGT GGGGAGAAGA TGACGCTCTG CATCTCAGTG 
CTGCTGGCAC TCACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCTCC CACCTCCCTT 
GACATTCCCC TCATTGGCAA GTACCTCTTG TTCACCATGG TGCTGGTCAC CTTTTCCATC 
GTCACCACTG TGTGTGTCCT CAATGTGCAC CACCGTTCAC CCAGCACTCA CACCATGGCA 
TCCTGGGTCA AGGAGTGCTT CCTGCACAAG CTGCCCACCT TCCTCTTCAT GAAGCGCCCT 
GGTCTGGAAG TCAGCCCCGC CAGGGTCCCT CATTCCAGCC AGCTGCACTT GACCACAGCT 
GAAGCTACGT CCACCTCTGC CTTAGGCCCC AGCAGCCCAT CCAACCTCTA TGGGAATTCC 
ATGTACTTTG TGAACCCTGT CCCTGCCACT CCTAAGTCTG CAGTCAGCTC CCACACGGCA 
GGCCTCCCCA GGGATGCCCG GCTGAGGTCC TCTGGGAGGT TCCGGCAAGA TCTACAGGAA 
GCATTAGAGG GCGTCAGCTT CATCGCACAG CATTTGGAGA GTGATGATCG AGATCAGAGT 
GTCATCGAGG ACTGGAAATT CGTCGCAATG GTCGTCGACC GCCTGTTCCT GTGGGTGTTC 
GTGATTGTGT GTATTCTGGG CACCATGGGG CTCTTCCTGC CACCCCTCTT CCAGATCCAC 
GCACCCTCCA AGGGCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510820.1
CDS	177..1577
Translation	MDDLLNKTRYNNLIRPATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAWNSSCYEGVNILRIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKECFLHKLPTFLFMKRPGLEVSPARVPHSSQLHLTTAEATSTSALGPSSPSNLYGNSMYFVNPVPATPKSAVSSHTAGLPRDARLRSSGRFRQDLQEALEGVSFIAQHLESDDRDQSVIEDWKFVAMVVDRLFLWVFVIVCILGTMGLFLPPLFQIHAPSKGL

Target ORF information:

RefSeq Version	XM_006510757.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4 (Chrnb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510757.4

ORF Insert Sequence:

ATGGATGATC TCCTGAACAA AACCCGCTAC AACAACCTGA TCCGCCCAGC CACCAGCTCC 
TCCCAGCTCA TCTCCATCCG CCTGGAGCTA TCACTGTCCC AGCTCATCAG TGTGAATGAG 
CGAGAACAGA TCATGACCAC CAGCATCTGG CTGAAACAGG AATGGACTGA CTACCGCCTG 
GCCTGGAACA GCTCCTGCTA TGAAGGGGTG AACATTCTGA GGATTCCTGC AAAGCGAGTC 
TGGTTGCCTG ACATCGTGTT GTACAACAAT GCCGATGGGA CCTATGAGGT GTCTGTCTAC 
ACCAACGTGA TCGTGCGTTC CAACGGCAGC ATCCAGTGGC TGCCCCCTGC CATCTACAAG 
AGTGCCTGCA AGATAGAGGT GAAGCACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAA 
TTTCGCTCCT GGACCTATGA CCACACAGAG ATTGACATGG TTCTTAAATC ACCCACAGCC 
ATCATGGATG ACTTTACCCC CAGCGGTGAA TGGGACATTG TGGCCCTCCC GGGTCGAAGG 
ACAGTGAACC CACAGGACCC CAGCTATGTG GACGTGACCT ATGACTTCAT CATCAAGCGC 
AAGCCACTCT TCTACACCAT CAATCTGATC ATCCCTTGTG TGCTCATCAC GTCGCTGGCT 
ATCCTGGTCT TCTACCTGCC CTCCGACTGT GGGGAGAAGA TGACGCTCTG CATCTCAGTG 
CTGCTGGCAC TCACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCTCC CACCTCCCTT 
GACATTCCCC TCATTGGCAA GTACCTCTTG TTCACCATGG TGCTGGTCAC CTTTTCCATC 
GTCACCACTG TGTGTGTCCT CAATGTGCAC CACCGTTCAC CCAGCACTCA CACCATGGCA 
TCCTGGGTCA AGGAGTGCTT CCTGCACAAG CTGCCCACCT TCCTCTTCAT GAAGCGCCCT 
GGTCTGGAAG TCAGCCCCGC CAGGGTCCCT CATTCCAGCC AGCTGCACTT GACCACAGCT 
GAAGCTACGT CCACCTCTGC CTTAGGCCCC AGCAGCCCAT CCAACCTCTA TGGGAATTCC 
ATGTACTTTG TGAACCCTGT CCCTGCCACT CCTAAGTCTG CAGTCAGCTC CCACACGGCA 
GGCCTCCCCA GGGATGCCCG GCTGAGGTCC TCTGGGAGGT TCCGGCAAGA TCTACAGGAA 
GCATTAGAGG GCGTCAGCTT CATCGCACAG CATTTGGAGA GTGATGATCG AGATCAGAGT 
GTCATCGAGG ACTGGAAATT CGTCGCAATG GTCGTCGACC GCCTGTTCCT GTGGGTGTTC 
GTGATTGTGT GTATTCTGGG CACCATGGGG CTCTTCCTGC CACCCCTCTT CCAGATCCAC 
GCACCCTCCA AGGGCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

No evidence of a role of the ?4 subunit of the nicotinic acetylcholine receptor in alcohol-related behaviors.
BMC research notes10(1)151(2017 Apr)
Kamens HM,Silva C,McCarthy R,Cox RJ,Ehringer MA

Role of ?4* Nicotinic Acetylcholine Receptors in the Habenulo-Interpeduncular Pathway in Nicotine Reinforcement in Mice.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology41(7)1790-802(2016 06)
Harrington L,Vi?als X,Herrera-Sol?s A,Flores A,Morel C,Tolu S,Faure P,Maldonado R,Maskos U,Robledo P

MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes.
Cell reports14(8)1991-2002(2016 Mar)
Ganic E,Singh T,Luan C,Fadista J,Johansson JK,Cyphert HA,Bennet H,Storm P,Prost G,Ahlenius H,Renstr?m E,Stein R,Groop L,Fex M,Artner I

Changes in Nicotinic Neurotransmission during Enteric Nervous System Development.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(18)7106-15(2015 May)
Foong JP,Hirst CS,Hao MM,McKeown SJ,Boesmans W,Young HM,Bornstein JC,Vanden Berghe P

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Chrnb4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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