Cenpf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cenpf
Entrez Gene ID	108000
Full Name	centromere protein F
Synonyms	6530404A22Rik,AI325968,CENF,Lek1,PRO1779,mitosin
General protein information	Preferred Names centromere protein F Names centromere protein F centromere autoantigen F leucine, glutamic acid, lysine family 1 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H6\|1 95.03 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320925.1	XP_017176414.1	centromere protein F isoform X1
XM_006497119.2	XP_006497182.1	centromere protein F isoform X1
NM_001081363.2	NP_001074832.2	centromere protein F
XM_006497119.3	XP_006497182.1	centromere protein F isoform X1

Pathways from BioSystems

Homologies

	CENPF	XP_001105956.2
Xenopus tropicalis (tropical clawed frog)	cenpf	XP_002934738.2
Homo sapiens (human)	CENPF	NP_057427.3
Pan troglodytes (chimpanzee)	CENPF	XP_001171564.1
Canis lupus familiaris (dog)	CENPF	XP_003639228.1
Mus musculus (house mouse)	Cenpf	NP_001074832.2
Bos taurus (cattle)	CENPF	NP_001243515.1
Gallus gallus (chicken)	CENPF	NP_989976.2
Rattus norvegicus (Norway rat)	Cenpf	NP_001094297.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities.
Pfaltzgraff ER, Roth GM, Miller PM, Gintzig AG, Ohi R, Bader DM
Molecular biology of the cell27(13)1990-9(2016 07)

Predicting Drug Response in Human Prostate Cancer from Preclinical Analysis of In Vivo Mouse Models.
Mitrofanova A, Aytes A, Zou M, Shen MM, Abate-Shen C, Califano A
Cell reports12(12)2060-71(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data show that mutation of the CENP-F gene leads to an unexpected hyperstabilization of the MT network, with a unique loss of dynamic instability. With disruption of MT dynamics, CENP-F-/- cells exhibit dramatic loss of directionally persistent migration, defects in focal adhesion disassembly and lamellipodial formation/retraction, change in cilia frequency, and loss of regulation of cell shape.
Title: Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities.

FOXM1 and CENPF are master regulators of prostate cancer malignancy, and can serve as drug response markers for antineoplastic drugs efficiency.
Title: Predicting Drug Response in Human Prostate Cancer from Preclinical Analysis of In Vivo Mouse Models.

Loss of CENP-F function in developing myocytes leads to decreased cell division, blunting of trabeculation and an initially smaller, thin-walled heart.
Title: Cardiac-specific deletion of the microtubule-binding protein CENP-F causes dilated cardiomyopathy.

Data show that the post-anaphase, KEN-box-dependent degradation of Cenp-F requires it to be farnesylated, a post-translational modification usually linked to membrane association.
Title: Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F.

Results identify Hook2, a linker protein that is essential for regulation of the microtubule network at the centrosome, as a binding partner of CENP-F.
Title: Murine CENP-F regulates centrosomal microtubule nucleation and interacts with Hook2 at the centrosome.

The following Cenpf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cenpf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02215	NM_001081363.2 Latest version!	Mus musculus centromere protein F (Cenpf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu28933	XM_017320925.1 Latest version!	Mus musculus centromere protein F (Cenpf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu28933	XM_006497119.3 Latest version!	Mus musculus centromere protein F (Cenpf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu28933	XM_006497119.2	Mus musculus centromere protein F (Cenpf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cenpf ( NM_001081363.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074832.2 Mus musculus centromere protein F (Cenpf), mRNA.

Price & Availability↑

CloneID	OMu02215
Clone ID Related Accession (Same CDS sequence)	NM_001081363.2
Accession Version	NM_001081363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-12
Organism	Mus musculus(house mouse)
Product	centromere protein F
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC140679.4. On Jun 6, 2008 this sequence version replaced NM_001081363.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: DQ642022.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGCTGGG CCCTGGAAGA ATGGAAGGAA GGTCTCCCCT CCAGAGCTCT TCAGAAGATC 
CAAGAGCTTG AAGGACAGCT GGAGAAGCTG AAGAAGGAGA AACAACAGAG GCAGTTCCAG 
CTGGACTCTC TCGAAGCTGC GCTGCAGAAG CAGAAGCAGA AGGTTGAAGA CGGAAAGACT 
GAGGGTGCAG ACCTGAAAAG GGAAAATCAA AGGTTGATGG AGATATGCGA ACATTTGGAG 
AAGTCAAGGC AGAAGCTGTC TCATGAACTT CAAGTTAAGG AGTCACAAGT GAATCTCCAA 
GAGAGCCAAC TGAGCTCATG CAAAAAGCAA ATAGAAAAAC TGGAACAGGA ACTTAAGCGG 
TGTAAATCTG AATTTGAAAG AAGCCAACAA GTTGCACAAT CGGCAGATGT TTCTCTGAAT 
CCATGCAGTA CACCACAGAA ACTCTTTGCA ACTCCACTCA CACCGAGTTC CACATACGAA 
GATCTGAAAG AAAAATATAA TAAAGAAGTT GAAGAACGGA AGAGGTTAGA GGAAGAGGTT 
AAAGCTTTGC ATGCAAAAAA AGTGAGCCTG CCTGTTTCCC AAGCCACCAT GAACCACCGG 
GACATTGCGA GACATCAGGC TTCCTCATCA GTGTTTCCTT GGCAACAGGA AAATACCCCA 
AGTCGCCTTT CATCGGATGC TCTGAAAACC CCACTGAGGA GAGACGGCTC TGCTGCTCAC 
TTTTTGGGGG AAGAAGTGAG TCCTAACAAA TCAAGTATGA AGACAGGGAG AGGAGACTGC 
AGCAGCCTCC CTGGTGAGCC TCACAGCGCT CAGCTTTTGC ACCAGGCCAA AGCCCAGAAT 
CAAGACCTAA AAAGCAAGAT GACTGAGTTA GAACTACGCC TGCAAGGGCA AGAAAAGGAA 
ATGAGAAGCC AAGTGAATAA ATGTCAAGAC TTACAGCTAC AGCTGGAGAA GACGAAAGTG 
GAATTGATTG AAAAGGAGAG AATTTTGAAT AAAACCAGAG ATGAAGTAGT GAGAAGCACA 
GCACAGTATG ACCAGGCCGC AGCCAAGTGT ACTACCTTGG AACAAAAGCT GAAAACTTTG 
ACTGAGGAGT TGAGTTGTCA CCGACAGAAC GCAGAGAGTG CTAAACGTTC TCTGGAACAG 
AGGATTAAGG AGAAAGAAAA GGAGCTTCAA GAGGAGCTGT CCCGACAGCA TCAATCTTTC 
CAAGCTCTGG ACAGTGAGTA CACTCAGATG AAAACCAGAC TTACCCAGGA GTTACAGCAA 
GTCAAGCATT TGCACAGCAC CCTCCAGCTG GAACTGGAGA AGGTCACATC AGTGAAGCAG 
CAGTTAGAAA GGAATTTGGA AGAAATTAGG CTTAAGTTGA GCAGAGCAGA ACAAGCTCTT 
CAGGCAAGTC AGGTCGCAGA AAACGAGCTG AGGAGAAGCA GTGAGGAAAT GAAGAAGGAG 
AACAGTCTCA TTAGGAGTCA GTCTGAGCAG AGGACCAGAG AAGTCTGCCA CCTGGAGGAA 
GAACTTGGTA AAGTCAAAGT GTCTTTGAGT AAGAGCCAGA ATTTTGCAGA AGAAATGAAG 
GCTAAGAATA CCTCTCAGGA AATCATGTTA CGAGATCTTC AGGAAAAACT AAATCAGCAA 
GAAAACTCAC TAACTTTAGA GAAGCTGAAA CTTGCCCTAG CTGATCTGGA AAGACAGCGA 
AACTGTTCTC AAGATCTCTT GAAGAAAAGG GAACATCACA TTGATCAACT GAATAATAAG 
TTAAATAAGA TAGAGAAAGA GTTTGAAACT TTGCTGAGTG CTTTGGAATT AAAAAAGAAA 
GAATGTGAAG AATTGAAAGA AGAGAAAAAT CAGATTTCTT TTTGGAAAAT TGATAGTGAA 
AAACTCATAA ATCAGATAGA ATCAGAAAAA GAAATCTTAT TGGGTAAAAT TAACCACTTA 
GAGACCAGCC TCAAGACACA ACAAGTAAGT CCTGACTCTA ATGAGAGAAT AAGAACACTG 
GAGATGGAAA GAGAAAACTT TACTGTGGAG ATTAAAAACC TTCAAAGTAT GTTAGACAGC 
AAGATGGTAG AGATCAAGAC ACAGAAACAA GCTTACTTGG AACTGCAGCA GAAATCCGAA 
TCCTCGGACC AAAAGCATCA GAAGGAGATA GAGAATATGT GCTTGAAAGC AAATAAGCTC 
ACTGGGCAAG TTGAAAGTTT GGAATGTAAG CTTCAGTTAT TGTCAAGTGA AGTAGTGACC 
AAAGACCAGC AGTACCAAGA CTTGCGTATG GAATATGAGA CACTGAGGGA TTTGCTCAAG 
TCCAGAGGAT CTTCTCTGGT GACAAATGAG GATAATCAGC GAAGTTCTGA GGATAATCAG 
AGAAGTTCTG AGGATAATCA GAGAGGCTCT TTGGCTTTTG AACAGCAGCC TGCAGTGAGT 
GATTCCTTTG CAAATGTAAT GGGGAGAAAA GGAAGCATAA ATTCAGAAAG GAGTGACTGC 
TCTGTAGATG GGGGCCGAAG TCCAGAACAT ATAGCCATCT TACAAAATAG AGTCACTTCA 
CTTGAAAGTT CCTTGGAGTC TCAAAACCAG ATGAATTCAG ATTTGCAAAT GCGGTGTGAA 
GAGTTGCTAC AAATCAAAGG GGAAGTAGAA GAAAACCTCA GTAAAGCAGA GCAGATTCAT 
CAGAATTTTG TGGCTGAAAC AAATCAATGT ATTAGTAAAT TGCAGGAAGA TGCTGCAGTT 
CATCAGAATA TTGTTGCTGA GACTTTAGCA ACCCTTGAGA GTAAGGAAAA AGAGTTACAG 
CTTTTGAAAG AAAAATTAGA AGCTCAGCAA ACAGAGGTTC AAAAGTTAAA TAAGAATAAC 
TGTCTTCTTG AAGGTACTCT GAAGGAGCTA CAGCTTTTAT CTGACACTCT GAGCTCAGAG 
AAGAAGGAAA TGAATTCTAT CATCTCATTA AGTAAAAAAA ACATTGAAGA GTTAACCCAA 
GCAAACGAGG CTCTCAAGGA AGTTAATGAG GCCTTAGAGC AGGAGAAAAT GAATTTACTC 
CAAAAGCATG AGAAGATTAC AAGCTGTATA GCAGAACAAG AGAGAAGCAT TGCAGAGCTG 
TCTGATCAGT ACAAGCAAGA AAGACTTCAA TTATTACAAC GGTGTGAAGA AACAGAAGCT 
GTGTTGGAAG ATCTCAGGGG AAACTACAAA ACAGCACAAG AAAACAATGC TAAGTTAGAA 
TGCATGCTCA GCGAGTGCAC TGCTCTTTGT GAAAATAGAA AAAATGAACT GGAGCAGTTA 
AAGGAAACAT TTGCAAAGGA ACAGCAAGAA TTCTTAACAA AATTAGCTTT CGCTGAAGAG 
CAAAACAGGA AACTAATGCT AGAGTTGGAG ATAGAGCAAC AAACTGTGAG ATCCGAGATT 
ACAAACACCA ACAAGCATTC CATGAGTGCG ACTGATGGCT TAAGGCAAGA ATGCTTGACT 
TTAAACGAAG AGCAAAATGA GCAGCAAAAC GAAGTTAGCA ACTTAACACA TGAGAATGAG 
CAACTGATGG AGTTAACACA GACCAAACAT GATTCTTATC TCGCAGTAGA GCCAGTTGAG 
AACTCTGTAA AAGCAACCGA AGATGAGATA GGTAAGAGTA GTTCCCAGTA CCAGATGGAT 
ATCGACACTA AAGACATTTC TCTAGATAGT TATAAGGCAC AGCTGGTACA TCTAGAAGCT 
TTGGTAAGAA TTCTGGAAGT ACAGCTTGAC CAAAGTGAGG AGGAGAACAA GAAGCTGCAT 
CTGGAATTAC AGACGATTAG AGAGGAGCTA GAAACCAAGA GTTCACAGGA CCCCCAGTCA 
CAGGCAAGGA CTGGGCTTAA AGACTGTGAC ACAGCAGAAG AAAAGTATGT GTCCATGCTA 
CAGGAGTTGT CAGCAAGTCA AAACGAGAAT GCACACTTAC AGTGCTCTCT ACAGACAGCA 
GTGAACAAAC TGAATGAGCT AGGGAAAATG TGTGACGTAT TGAGAGTTGA AAAGTTACAG 
CTAGAGTCTG AGCTGAATGA CTCACGGACA GAGTGTATCA CGGCAACTAG TCAGATGACG 
GCAGAGGTCG AGAAGCTAGT GAGTGAAATG AAAATGCTAA ACCACGAGAG TGCTCTGTCC 
CAGAATGAGC TGATGAAGGA CACCTCAGGT GGTGAATTTC ATGATAAAGC AAACCACAGT 
TCTGTGTTCT TGACTCCTTT GGACAGTAGC AATTTCTGTG AACAGATGAC CTTGTCAAGC 
AAAGAGGTCC GAGTGCACTT TGCTGAATTA CAGGAGAAAT TCTCCTGTTT ACAAAGTGAA 
CACAAAATTT TACATGATCA GCACTGTGAG GTGAGCTCTA AGATGTCAGC ACTGCGTTCC 
TACGTGGACA CATTAAAAGC TGAAAATTCT GCCTTGTCAA TGAGTCTGAG AACCTTGCAG 
GGTGACTTGG TAAAGGAGGG GGAGCCTGCA GCTGAGGGTG GGCATGGTCT GCCACTGTCG 
TTCTGTGGGG CAGACAGCCC GTCTCTGACA AATTTTGGAG AAACGTCCTT TTACAAAGAC 
GTTTTAGAAC AAACTGGAGA CACATGTCAT CTAAGTTTAG AAGGGAACGC TTCAGCAAAT 
TCTTGTGACT TGGATGAAGA GTTCTCCAGC AGTCTGGAGG AGGAGACTCT GACTGAGAAG 
GAAAGCCCAC CTGCCCCTGG GAGGACTGTT GAGGGACTTG AAGTCCTATG CCAGGTGTAC 
TTGCAGTCCC TCAAGAATCT AGAGGAGAAA ACTGAGAGCC AAAGGATTAT GAAAAATAAA 
GAAATTGAAA AGCTTGAGCA GTTACTGAGT TCTGAGAGGA AAGAGCTAAG CTGCCTTAGG 
AAGCAGTATT TGTCAGAAAA GGAGCAATGG CAGCAGAAGC TAACAAGTGT CACTTTGGAA 
ATGGAGTCCA AGTTAGCAGA GGAGAAGCAG CAGACCAAGA CTCTGTCCCT TGAACTTGAG 
GTAGCACGAC TTCAGTTACA GGAGCTGGAC CTGAGCTCCA GGTCTTTGCT TGGCACTGAC 
TTGGAAAGTG TTGTTCGGTG CCAAAACGAT AATTATGATA TAAAAGAATC AGAAGTATAT 
ATTTCAGAGA CTACAGAGAA AACACCAAAG CAGGACACTG ACCAAACTTG TGATAAAGAT 
ATTCAGCAGG ACCTTGGTCT GGAAACTTCA GTCACTGAGA GTGAGACTAC CAGGCTCACA 
GGAGAGGGGT GTGAAGAGCA GCCTCCGAAG ACCAATTGTG AGGCACCAGC GGAGGACAAA 
ACCCAGGACT GCTCAGAATG CATTTCTGAA TTGTGTTCTA GTTCCAATGT TTTGGTGCCC 
ATGGATGTTC TGGAAGATCA GGGGTCTATC CAGAATCTCC AGTTGCAGAA AGACACCTTA 
AATGAGAATT TGAGATTACT TCCTGAAGTA GAGGACTGGG ACAAAAAAGT TGAAAGTTTG 
CTAAATGAAA TTATGGAGGC AGATTCAAAA CTGAGTTTAC AGGAAGTACA GCTCAAGATG 
AAGATTGCAA CATGCATACA ATTGGAAAAA ATAGTCAAGG ACCTCAGAAA GGAAAAAGCT 
GACTTAAGTG AAAAGTTGGA ATCCCTTCCA TGTAACCAGG AGGTATGTCT GAGAGTAGAA 
AGGTCAGAAG AAGATCTTGG TTTTAATTTA GATATGGGAG CAAATGAGTT GTTAAGTAAA 
TCTACTAAAG ATAATGCAAC CAACACAGAA GACAATTATA AGGAGAAGTT TCTTGATATG 
GAAAGAGAAC TGACCAGAAT TAAGTCTGAG AAAGCTAATA TTGAGCATCA CATCCTATCT 
GTGGAAACTA ACTTAGAGGT GGTTCAAGCA GAGAAGCTCT GTTTGGAAAG AGACACTGAA 
AGTAAGCAAA AGGTTATTAT TGACCTTAAA GAAGAACTAT TTACAGTTAT AAGTGAGAGA 
AACAGACTTC GGGAAGAATT AGATAATGTG TCAAAAGAAA GCAAAGCACT GGATCAGATG 
TCTAAAAAGA TGAAAGAGAA AATAGAAGAG CTGGAGTCTC ACCAAAGGGA GAGCCTCCGT 
CACATTGGGG CAGTAGAGTC TGAGGTCAAG GACAAAGCAG ATCTTATTCA GACTCTGTCC 
TTTAATGTGG GTGAGCTAAC AAAAGACAAA GCTCATCTCC AGGAGCAGCT GCAGAATTTG 
CAGAATGACT CACAAGAATT ATCTTTGGCG ATTGGTGAGC TGGAAATACA AATTGGACAA 
CTGAATAAAG AGAAAGAATC ACTGGTCAAG GAGTCTCAGA ACTTCCAGAT CAAGCTGACT 
GAGTCAGAGT GTGAAAAGCA GACGATCTCT AAGGCCTTGG AGGTGGCACT CAAGGAGAAA 
GGTGAGTTTG CAGTGCAGCT GAGCTCAGCC CAGGAGGAGG TGCATCAGCT GAGACGAGGC 
ATTGAGAAAC TGAGCGTCCG CATTGAGGCC GATGAGAAGA AGCACCTCAG TGCTGTGGCG 
AAGCTGAAAG AAAGCCAGCG TGAAAGCGAC TCATTGAAGG ATACAGTGGA GACTCTGGAG 
CGGGAACTGG AGAGGTCAGA AGAAAACCAA GAGCTGGCAA TTCTTGATTC TGAGAATTTG 
AAAGCAGAGG TGGAGACCCT TAAGGCACAA AAGGATGAAA TGACCAAAAG CCTGAGAATT 
TTCGAATTAG ACCTTGTTAC AGTTAGGACT GAAAGAGAAA ATCTAGCAAA GCAGCTACAA 
GAGAAACAAA GTCGAGTGTC AGAATTAGAT GAACGGTGCT CTTCCTTGAG AAGACTGTTG 
GAAGAGAAGG AGCAAGCAAG AGTACAGATG GAAGAAGACT CTAAGTCTGC AATGCTGATG 
CTTCAGATGC AGTTAAAAGA ACTCAGGGAG GAAGTGGCAG CCTTGTGTAA TGACCAAGAA 
ACCTTGAAGG CCCAAGAACA GAGTCTAGAC CAACCAGGGG AGGAAGTGCA TCATTTGAAA 
AGTAGCATTC GAAAGCTCAA AGTTCACATA GATGCTGATG AAAAGAAGCA TCAAAACATC 
CTAGAACAAC TGAAGGAAAG TAAGCACCAT GCAGACTTGC TTAAGGACCG AGTTGAGAAC 
CTTGAACAAG AATTGATACT ATCAGAGAAA AACATGATTT TCCAAGCTGA AAAGTCCAAA 
GCAGAGATAC AGACTTTAAA ATCAGAAATT CAAAGAATGG CCCAAAACCT CCAAGACTTG 
CAGTTAGAAC TTATTAGTAC AAGGTCAGAA AATGAAAATC TCATGAAAGA ATTAAAAAAA 
GAGCAAGAGC GAGTATCTGA CTTAGAAACA ATAAATTCTT CTATTGAAAA CTTACTGAAA 
GATAAAGAGC AAGAAAAAGT ACAGATGAAA GAGGAAGCCA AAATAACAGT GGAGATGCTT 
CAAACTCAAT TAAAGGAGCT AAACGAGACA GTGGTTTCCT TGTGCAATGA CCAAGAGGTC 
TCTAAGACCA AAGAACAGAA TCTGGGTAGT CAAGTACAAA CTCTTGAACT TGAGAAGGCT 
CAGCTGCTAC AGGACCTTGG TGAGGCCAAG AATAAATATA TTATTTTTCA GTCATCTGTA 
AATGCCCTCA CTCAAGAAGT AGAAGCTGGC AAACAGAAAC TAGAGAAGGG GGAGAAAGAG 
ATCAGGACAC TGAAAGAGCA ACTTAAAAGT CAGGAGCAGC TTGTGTGTAA ACTTGCCCAA 
GTGGAAGGAG AGCAGCAACT CTGGCAGAAG CAGAAACTAG AGCTGAGAAA TGTGACTATG 
GCACTGGAGC AGAAGGTCCA AGTGCTGCAA TCTGAAAACA ACACGTTGCA GAGCACCTAT 
GAAGCACTGC AGAATTCCCA CAAGAGTTTA GAGAGTGAAC TTGGATTGAT AAAGTTGGAG 
AAAGTAGCGC TTGTTGAAAG AGTTAGCACA ATATCTGGGA AGGAAGCAGA GCTGCAGAGG 
GAGCTGCGAG ATATGCTACA GAAAACAACA CAGCTGAGCG AAGACTACAA TAAAGAGAAA 
AACAGGCTAA CAGAAGAAGT GGAAGTGCTG CGTGAAGAAC TGCAGAACAC CAAAGCAGCG 
CACCTGAAAT CTGTGAATCA ACTTGAGAAG GAACTTCAGC GTGCTCAGGG GAAAATAAAG 
TTGATGCTCA AATCCTGTAG ACAGCTGGAA GGAGAAAAGG AGATGCTGCA GAAGGAGCTC 
TCCCAGCTTG AAGCTGCACA GCAGCAGAGA GCAGGTTCTC TTGTAGACAG TAACGTAGAT 
GAAGTAATGA CTGAGAACAA AGCGCTGAAA GAGACTCTGG AAGAAAAAGT CAAGGAAGCA 
GATAAGTACT TGGATAAGTA CTGTTCCCTG CTGATAAGCC ACGAGGAGCT CGAGAAAGCC 
AAGGAGATAT TAGAAATAGA AGTTGCTCGG CTGAAGTCAC GGCAGTCCAG ACAGGATCTC 
CAGAGTTCTC CTTTGCTTAA TTCTTCCATT CCAGGACCGT CTCCAAATAC TTCTGTTAGT 
GAGATGAAGT CAGCATCTGG CCAAAATAAA GCTTCAGGCA AGAGGCAAAG GTCCAGTGGG 
ATTTGGGAGC ATGGTAAACG GGCAGCACCT TCTACAGCAG AGACATTTTC TAAGAAAAGC 
AGGAAGTCGG ACAGTAAGAG CACTCGCCCT GCTGAGCACG AGCAGGAAAC CGAGTTTGAG 
CCAGAAGGCC TCCCAGAAGT CGTTAAAAAA GGGTTTGCTG ACATCCCAAC TGGAAAGACA 
AGCCCATATA TCCTTCGGAG AACAACCATG GCAACCAGGA CCAGCCCCCG CTTTGCTACA 
CAGAAGTTAG TGGGATCTTC CCCATCTCTG GGCAAAGAAA ATGTTGTAGA GTCCTCCAAA 
CCAACAGCTG GTGGCAGCAG ATCACAAAAG GTCAAGGTTG TTCAGGAGAG CTCAGCGGAT 
TCACACACTG CCTTCCAAGA ACTCCCAGCA AAATCTCTCA CAGCCAGTAA TATTCCTGGG 
AGAAACTCTA CAGAGAGCCC CAGGGAGGGC CTGAGGGCCA AGCGGGCCTA CCCTGCCTCC 
AGCCCAGCTG CTGGGCCTGA TCCCACGAAC AACGAAAACT GCCGGGTCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074832.2
CDS	144..9137
Translation	MSWALEEWKEGLPSRALQKIQELEGQLEKLKKEKQQRQFQLDSLEAALQKQKQKVEDGKTEGADLKRENQRLMEICEHLEKSRQKLSHELQVKESQVNLQESQLSSCKKQIEKLEQELKRCKSEFERSQQVAQSADVSLNPCSTPQKLFATPLTPSSTYEDLKEKYNKEVEERKRLEEEVKALHAKKVSLPVSQATMNHRDIARHQASSSVFPWQQENTPSRLSSDALKTPLRRDGSAAHFLGEEVSPNKSSMKTGRGDCSSLPGEPHSAQLLHQAKAQNQDLKSKMTELELRLQGQEKEMRSQVNKCQDLQLQLEKTKVELIEKERILNKTRDEVVRSTAQYDQAAAKCTTLEQKLKTLTEELSCHRQNAESAKRSLEQRIKEKEKELQEELSRQHQSFQALDSEYTQMKTRLTQELQQVKHLHSTLQLELEKVTSVKQQLERNLEEIRLKLSRAEQALQASQVAENELRRSSEEMKKENSLIRSQSEQRTREVCHLEEELGKVKVSLSKSQNFAEEMKAKNTSQEIMLRDLQEKLNQQENSLTLEKLKLALADLERQRNCSQDLLKKREHHIDQLNNKLNKIEKEFETLLSALELKKKECEELKEEKNQISFWKIDSEKLINQIESEKEILLGKINHLETSLKTQQVSPDSNERIRTLEMERENFTVEIKNLQSMLDSKMVEIKTQKQAYLELQQKSESSDQKHQKEIENMCLKANKLTGQVESLECKLQLLSSEVVTKDQQYQDLRMEYETLRDLLKSRGSSLVTNEDNQRSSEDNQRSSEDNQRGSLAFEQQPAVSDSFANVMGRKGSINSERSDCSVDGGRSPEHIAILQNRVTSLESSLESQNQMNSDLQMRCEELLQIKGEVEENLSKAEQIHQNFVAETNQCISKLQEDAAVHQNIVAETLATLESKEKELQLLKEKLEAQQTEVQKLNKNNCLLEGTLKELQLLSDTLSSEKKEMNSIISLSKKNIEELTQANEALKEVNEALEQEKMNLLQKHEKITSCIAEQERSIAELSDQYKQERLQLLQRCEETEAVLEDLRGNYKTAQENNAKLECMLSECTALCENRKNELEQLKETFAKEQQEFLTKLAFAEEQNRKLMLELEIEQQTVRSEITNTNKHSMSATDGLRQECLTLNEEQNEQQNEVSNLTHENEQLMELTQTKHDSYLAVEPVENSVKATEDEIGKSSSQYQMDIDTKDISLDSYKAQLVHLEALVRILEVQLDQSEEENKKLHLELQTIREELETKSSQDPQSQARTGLKDCDTAEEKYVSMLQELSASQNENAHLQCSLQTAVNKLNELGKMCDVLRVEKLQLESELNDSRTECITATSQMTAEVEKLVSEMKMLNHESALSQNELMKDTSGGEFHDKANHSSVFLTPLDSSNFCEQMTLSSKEVRVHFAELQEKFSCLQSEHKILHDQHCEVSSKMSALRSYVDTLKAENSALSMSLRTLQGDLVKEGEPAAEGGHGLPLSFCGADSPSLTNFGETSFYKDVLEQTGDTCHLSLEGNASANSCDLDEEFSSSLEEETLTEKESPPAPGRTVEGLEVLCQVYLQSLKNLEEKTESQRIMKNKEIEKLEQLLSSERKELSCLRKQYLSEKEQWQQKLTSVTLEMESKLAEEKQQTKTLSLELEVARLQLQELDLSSRSLLGTDLESVVRCQNDNYDIKESEVYISETTEKTPKQDTDQTCDKDIQQDLGLETSVTESETTRLTGEGCEEQPPKTNCEAPAEDKTQDCSECISELCSSSNVLVPMDVLEDQGSIQNLQLQKDTLNENLRLLPEVEDWDKKVESLLNEIMEADSKLSLQEVQLKMKIATCIQLEKIVKDLRKEKADLSEKLESLPCNQEVCLRVERSEEDLGFNLDMGANELLSKSTKDNATNTEDNYKEKFLDMERELTRIKSEKANIEHHILSVETNLEVVQAEKLCLERDTESKQKVIIDLKEELFTVISERNRLREELDNVSKESKALDQMSKKMKEKIEELESHQRESLRHIGAVESEVKDKADLIQTLSFNVGELTKDKAHLQEQLQNLQNDSQELSLAIGELEIQIGQLNKEKESLVKESQNFQIKLTESECEKQTISKALEVALKEKGEFAVQLSSAQEEVHQLRRGIEKLSVRIEADEKKHLSAVAKLKESQRESDSLKDTVETLERELERSEENQELAILDSENLKAEVETLKAQKDEMTKSLRIFELDLVTVRTERENLAKQLQEKQSRVSELDERCSSLRRLLEEKEQARVQMEEDSKSAMLMLQMQLKELREEVAALCNDQETLKAQEQSLDQPGEEVHHLKSSIRKLKVHIDADEKKHQNILEQLKESKHHADLLKDRVENLEQELILSEKNMIFQAEKSKAEIQTLKSEIQRMAQNLQDLQLELISTRSENENLMKELKKEQERVSDLETINSSIENLLKDKEQEKVQMKEEAKITVEMLQTQLKELNETVVSLCNDQEVSKTKEQNLGSQVQTLELEKAQLLQDLGEAKNKYIIFQSSVNALTQEVEAGKQKLEKGEKEIRTLKEQLKSQEQLVCKLAQVEGEQQLWQKQKLELRNVTMALEQKVQVLQSENNTLQSTYEALQNSHKSLESELGLIKLEKVALVERVSTISGKEAELQRELRDMLQKTTQLSEDYNKEKNRLTEEVEVLREELQNTKAAHLKSVNQLEKELQRAQGKIKLMLKSCRQLEGEKEMLQKELSQLEAAQQQRAGSLVDSNVDEVMTENKALKETLEEKVKEADKYLDKYCSLLISHEELEKAKEILEIEVARLKSRQSRQDLQSSPLLNSSIPGPSPNTSVSEMKSASGQNKASGKRQRSSGIWEHGKRAAPSTAETFSKKSRKSDSKSTRPAEHEQETEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRFATQKLVGSSPSLGKENVVESSKPTAGGSRSQKVKVVQESSADSHTAFQELPAKSLTASNIPGRNSTESPREGLRAKRAYPASSPAAGPDPTNNENCRVQ

Target ORF information:

RefSeq Version	NM_001081363.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centromere protein F (Cenpf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081363.2

ORF Insert Sequence:

ATGAGCTGGG CCCTGGAAGA ATGGAAGGAA GGTCTCCCCT CCAGAGCTCT TCAGAAGATC 
CAAGAGCTTG AAGGACAGCT GGAGAAGCTG AAGAAGGAGA AACAACAGAG GCAGTTCCAG 
CTGGACTCTC TCGAAGCTGC GCTGCAGAAG CAGAAGCAGA AGGTTGAAGA CGGAAAGACT 
GAGGGTGCAG ACCTGAAAAG GGAAAATCAA AGGTTGATGG AGATATGCGA ACATTTGGAG 
AAGTCAAGGC AGAAGCTGTC TCATGAACTT CAAGTTAAGG AGTCACAAGT GAATCTCCAA 
GAGAGCCAAC TGAGCTCATG CAAAAAGCAA ATAGAAAAAC TGGAACAGGA ACTTAAGCGG 
TGTAAATCTG AATTTGAAAG AAGCCAACAA GTTGCACAAT CGGCAGATGT TTCTCTGAAT 
CCATGCAGTA CACCACAGAA ACTCTTTGCA ACTCCACTCA CACCGAGTTC CACATACGAA 
GATCTGAAAG AAAAATATAA TAAAGAAGTT GAAGAACGGA AGAGGTTAGA GGAAGAGGTT 
AAAGCTTTGC ATGCAAAAAA AGTGAGCCTG CCTGTTTCCC AAGCCACCAT GAACCACCGG 
GACATTGCGA GACATCAGGC TTCCTCATCA GTGTTTCCTT GGCAACAGGA AAATACCCCA 
AGTCGCCTTT CATCGGATGC TCTGAAAACC CCACTGAGGA GAGACGGCTC TGCTGCTCAC 
TTTTTGGGGG AAGAAGTGAG TCCTAACAAA TCAAGTATGA AGACAGGGAG AGGAGACTGC 
AGCAGCCTCC CTGGTGAGCC TCACAGCGCT CAGCTTTTGC ACCAGGCCAA AGCCCAGAAT 
CAAGACCTAA AAAGCAAGAT GACTGAGTTA GAACTACGCC TGCAAGGGCA AGAAAAGGAA 
ATGAGAAGCC AAGTGAATAA ATGTCAAGAC TTACAGCTAC AGCTGGAGAA GACGAAAGTG 
GAATTGATTG AAAAGGAGAG AATTTTGAAT AAAACCAGAG ATGAAGTAGT GAGAAGCACA 
GCACAGTATG ACCAGGCCGC AGCCAAGTGT ACTACCTTGG AACAAAAGCT GAAAACTTTG 
ACTGAGGAGT TGAGTTGTCA CCGACAGAAC GCAGAGAGTG CTAAACGTTC TCTGGAACAG 
AGGATTAAGG AGAAAGAAAA GGAGCTTCAA GAGGAGCTGT CCCGACAGCA TCAATCTTTC 
CAAGCTCTGG ACAGTGAGTA CACTCAGATG AAAACCAGAC TTACCCAGGA GTTACAGCAA 
GTCAAGCATT TGCACAGCAC CCTCCAGCTG GAACTGGAGA AGGTCACATC AGTGAAGCAG 
CAGTTAGAAA GGAATTTGGA AGAAATTAGG CTTAAGTTGA GCAGAGCAGA ACAAGCTCTT 
CAGGCAAGTC AGGTCGCAGA AAACGAGCTG AGGAGAAGCA GTGAGGAAAT GAAGAAGGAG 
AACAGTCTCA TTAGGAGTCA GTCTGAGCAG AGGACCAGAG AAGTCTGCCA CCTGGAGGAA 
GAACTTGGTA AAGTCAAAGT GTCTTTGAGT AAGAGCCAGA ATTTTGCAGA AGAAATGAAG 
GCTAAGAATA CCTCTCAGGA AATCATGTTA CGAGATCTTC AGGAAAAACT AAATCAGCAA 
GAAAACTCAC TAACTTTAGA GAAGCTGAAA CTTGCCCTAG CTGATCTGGA AAGACAGCGA 
AACTGTTCTC AAGATCTCTT GAAGAAAAGG GAACATCACA TTGATCAACT GAATAATAAG 
TTAAATAAGA TAGAGAAAGA GTTTGAAACT TTGCTGAGTG CTTTGGAATT AAAAAAGAAA 
GAATGTGAAG AATTGAAAGA AGAGAAAAAT CAGATTTCTT TTTGGAAAAT TGATAGTGAA 
AAACTCATAA ATCAGATAGA ATCAGAAAAA GAAATCTTAT TGGGTAAAAT TAACCACTTA 
GAGACCAGCC TCAAGACACA ACAAGTAAGT CCTGACTCTA ATGAGAGAAT AAGAACACTG 
GAGATGGAAA GAGAAAACTT TACTGTGGAG ATTAAAAACC TTCAAAGTAT GTTAGACAGC 
AAGATGGTAG AGATCAAGAC ACAGAAACAA GCTTACTTGG AACTGCAGCA GAAATCCGAA 
TCCTCGGACC AAAAGCATCA GAAGGAGATA GAGAATATGT GCTTGAAAGC AAATAAGCTC 
ACTGGGCAAG TTGAAAGTTT GGAATGTAAG CTTCAGTTAT TGTCAAGTGA AGTAGTGACC 
AAAGACCAGC AGTACCAAGA CTTGCGTATG GAATATGAGA CACTGAGGGA TTTGCTCAAG 
TCCAGAGGAT CTTCTCTGGT GACAAATGAG GATAATCAGC GAAGTTCTGA GGATAATCAG 
AGAAGTTCTG AGGATAATCA GAGAGGCTCT TTGGCTTTTG AACAGCAGCC TGCAGTGAGT 
GATTCCTTTG CAAATGTAAT GGGGAGAAAA GGAAGCATAA ATTCAGAAAG GAGTGACTGC 
TCTGTAGATG GGGGCCGAAG TCCAGAACAT ATAGCCATCT TACAAAATAG AGTCACTTCA 
CTTGAAAGTT CCTTGGAGTC TCAAAACCAG ATGAATTCAG ATTTGCAAAT GCGGTGTGAA 
GAGTTGCTAC AAATCAAAGG GGAAGTAGAA GAAAACCTCA GTAAAGCAGA GCAGATTCAT 
CAGAATTTTG TGGCTGAAAC AAATCAATGT ATTAGTAAAT TGCAGGAAGA TGCTGCAGTT 
CATCAGAATA TTGTTGCTGA GACTTTAGCA ACCCTTGAGA GTAAGGAAAA AGAGTTACAG 
CTTTTGAAAG AAAAATTAGA AGCTCAGCAA ACAGAGGTTC AAAAGTTAAA TAAGAATAAC 
TGTCTTCTTG AAGGTACTCT GAAGGAGCTA CAGCTTTTAT CTGACACTCT GAGCTCAGAG 
AAGAAGGAAA TGAATTCTAT CATCTCATTA AGTAAAAAAA ACATTGAAGA GTTAACCCAA 
GCAAACGAGG CTCTCAAGGA AGTTAATGAG GCCTTAGAGC AGGAGAAAAT GAATTTACTC 
CAAAAGCATG AGAAGATTAC AAGCTGTATA GCAGAACAAG AGAGAAGCAT TGCAGAGCTG 
TCTGATCAGT ACAAGCAAGA AAGACTTCAA TTATTACAAC GGTGTGAAGA AACAGAAGCT 
GTGTTGGAAG ATCTCAGGGG AAACTACAAA ACAGCACAAG AAAACAATGC TAAGTTAGAA 
TGCATGCTCA GCGAGTGCAC TGCTCTTTGT GAAAATAGAA AAAATGAACT GGAGCAGTTA 
AAGGAAACAT TTGCAAAGGA ACAGCAAGAA TTCTTAACAA AATTAGCTTT CGCTGAAGAG 
CAAAACAGGA AACTAATGCT AGAGTTGGAG ATAGAGCAAC AAACTGTGAG ATCCGAGATT 
ACAAACACCA ACAAGCATTC CATGAGTGCG ACTGATGGCT TAAGGCAAGA ATGCTTGACT 
TTAAACGAAG AGCAAAATGA GCAGCAAAAC GAAGTTAGCA ACTTAACACA TGAGAATGAG 
CAACTGATGG AGTTAACACA GACCAAACAT GATTCTTATC TCGCAGTAGA GCCAGTTGAG 
AACTCTGTAA AAGCAACCGA AGATGAGATA GGTAAGAGTA GTTCCCAGTA CCAGATGGAT 
ATCGACACTA AAGACATTTC TCTAGATAGT TATAAGGCAC AGCTGGTACA TCTAGAAGCT 
TTGGTAAGAA TTCTGGAAGT ACAGCTTGAC CAAAGTGAGG AGGAGAACAA GAAGCTGCAT 
CTGGAATTAC AGACGATTAG AGAGGAGCTA GAAACCAAGA GTTCACAGGA CCCCCAGTCA 
CAGGCAAGGA CTGGGCTTAA AGACTGTGAC ACAGCAGAAG AAAAGTATGT GTCCATGCTA 
CAGGAGTTGT CAGCAAGTCA AAACGAGAAT GCACACTTAC AGTGCTCTCT ACAGACAGCA 
GTGAACAAAC TGAATGAGCT AGGGAAAATG TGTGACGTAT TGAGAGTTGA AAAGTTACAG 
CTAGAGTCTG AGCTGAATGA CTCACGGACA GAGTGTATCA CGGCAACTAG TCAGATGACG 
GCAGAGGTCG AGAAGCTAGT GAGTGAAATG AAAATGCTAA ACCACGAGAG TGCTCTGTCC 
CAGAATGAGC TGATGAAGGA CACCTCAGGT GGTGAATTTC ATGATAAAGC AAACCACAGT 
TCTGTGTTCT TGACTCCTTT GGACAGTAGC AATTTCTGTG AACAGATGAC CTTGTCAAGC 
AAAGAGGTCC GAGTGCACTT TGCTGAATTA CAGGAGAAAT TCTCCTGTTT ACAAAGTGAA 
CACAAAATTT TACATGATCA GCACTGTGAG GTGAGCTCTA AGATGTCAGC ACTGCGTTCC 
TACGTGGACA CATTAAAAGC TGAAAATTCT GCCTTGTCAA TGAGTCTGAG AACCTTGCAG 
GGTGACTTGG TAAAGGAGGG GGAGCCTGCA GCTGAGGGTG GGCATGGTCT GCCACTGTCG 
TTCTGTGGGG CAGACAGCCC GTCTCTGACA AATTTTGGAG AAACGTCCTT TTACAAAGAC 
GTTTTAGAAC AAACTGGAGA CACATGTCAT CTAAGTTTAG AAGGGAACGC TTCAGCAAAT 
TCTTGTGACT TGGATGAAGA GTTCTCCAGC AGTCTGGAGG AGGAGACTCT GACTGAGAAG 
GAAAGCCCAC CTGCCCCTGG GAGGACTGTT GAGGGACTTG AAGTCCTATG CCAGGTGTAC 
TTGCAGTCCC TCAAGAATCT AGAGGAGAAA ACTGAGAGCC AAAGGATTAT GAAAAATAAA 
GAAATTGAAA AGCTTGAGCA GTTACTGAGT TCTGAGAGGA AAGAGCTAAG CTGCCTTAGG 
AAGCAGTATT TGTCAGAAAA GGAGCAATGG CAGCAGAAGC TAACAAGTGT CACTTTGGAA 
ATGGAGTCCA AGTTAGCAGA GGAGAAGCAG CAGACCAAGA CTCTGTCCCT TGAACTTGAG 
GTAGCACGAC TTCAGTTACA GGAGCTGGAC CTGAGCTCCA GGTCTTTGCT TGGCACTGAC 
TTGGAAAGTG TTGTTCGGTG CCAAAACGAT AATTATGATA TAAAAGAATC AGAAGTATAT 
ATTTCAGAGA CTACAGAGAA AACACCAAAG CAGGACACTG ACCAAACTTG TGATAAAGAT 
ATTCAGCAGG ACCTTGGTCT GGAAACTTCA GTCACTGAGA GTGAGACTAC CAGGCTCACA 
GGAGAGGGGT GTGAAGAGCA GCCTCCGAAG ACCAATTGTG AGGCACCAGC GGAGGACAAA 
ACCCAGGACT GCTCAGAATG CATTTCTGAA TTGTGTTCTA GTTCCAATGT TTTGGTGCCC 
ATGGATGTTC TGGAAGATCA GGGGTCTATC CAGAATCTCC AGTTGCAGAA AGACACCTTA 
AATGAGAATT TGAGATTACT TCCTGAAGTA GAGGACTGGG ACAAAAAAGT TGAAAGTTTG 
CTAAATGAAA TTATGGAGGC AGATTCAAAA CTGAGTTTAC AGGAAGTACA GCTCAAGATG 
AAGATTGCAA CATGCATACA ATTGGAAAAA ATAGTCAAGG ACCTCAGAAA GGAAAAAGCT 
GACTTAAGTG AAAAGTTGGA ATCCCTTCCA TGTAACCAGG AGGTATGTCT GAGAGTAGAA 
AGGTCAGAAG AAGATCTTGG TTTTAATTTA GATATGGGAG CAAATGAGTT GTTAAGTAAA 
TCTACTAAAG ATAATGCAAC CAACACAGAA GACAATTATA AGGAGAAGTT TCTTGATATG 
GAAAGAGAAC TGACCAGAAT TAAGTCTGAG AAAGCTAATA TTGAGCATCA CATCCTATCT 
GTGGAAACTA ACTTAGAGGT GGTTCAAGCA GAGAAGCTCT GTTTGGAAAG AGACACTGAA 
AGTAAGCAAA AGGTTATTAT TGACCTTAAA GAAGAACTAT TTACAGTTAT AAGTGAGAGA 
AACAGACTTC GGGAAGAATT AGATAATGTG TCAAAAGAAA GCAAAGCACT GGATCAGATG 
TCTAAAAAGA TGAAAGAGAA AATAGAAGAG CTGGAGTCTC ACCAAAGGGA GAGCCTCCGT 
CACATTGGGG CAGTAGAGTC TGAGGTCAAG GACAAAGCAG ATCTTATTCA GACTCTGTCC 
TTTAATGTGG GTGAGCTAAC AAAAGACAAA GCTCATCTCC AGGAGCAGCT GCAGAATTTG 
CAGAATGACT CACAAGAATT ATCTTTGGCG ATTGGTGAGC TGGAAATACA AATTGGACAA 
CTGAATAAAG AGAAAGAATC ACTGGTCAAG GAGTCTCAGA ACTTCCAGAT CAAGCTGACT 
GAGTCAGAGT GTGAAAAGCA GACGATCTCT AAGGCCTTGG AGGTGGCACT CAAGGAGAAA 
GGTGAGTTTG CAGTGCAGCT GAGCTCAGCC CAGGAGGAGG TGCATCAGCT GAGACGAGGC 
ATTGAGAAAC TGAGCGTCCG CATTGAGGCC GATGAGAAGA AGCACCTCAG TGCTGTGGCG 
AAGCTGAAAG AAAGCCAGCG TGAAAGCGAC TCATTGAAGG ATACAGTGGA GACTCTGGAG 
CGGGAACTGG AGAGGTCAGA AGAAAACCAA GAGCTGGCAA TTCTTGATTC TGAGAATTTG 
AAAGCAGAGG TGGAGACCCT TAAGGCACAA AAGGATGAAA TGACCAAAAG CCTGAGAATT 
TTCGAATTAG ACCTTGTTAC AGTTAGGACT GAAAGAGAAA ATCTAGCAAA GCAGCTACAA 
GAGAAACAAA GTCGAGTGTC AGAATTAGAT GAACGGTGCT CTTCCTTGAG AAGACTGTTG 
GAAGAGAAGG AGCAAGCAAG AGTACAGATG GAAGAAGACT CTAAGTCTGC AATGCTGATG 
CTTCAGATGC AGTTAAAAGA ACTCAGGGAG GAAGTGGCAG CCTTGTGTAA TGACCAAGAA 
ACCTTGAAGG CCCAAGAACA GAGTCTAGAC CAACCAGGGG AGGAAGTGCA TCATTTGAAA 
AGTAGCATTC GAAAGCTCAA AGTTCACATA GATGCTGATG AAAAGAAGCA TCAAAACATC 
CTAGAACAAC TGAAGGAAAG TAAGCACCAT GCAGACTTGC TTAAGGACCG AGTTGAGAAC 
CTTGAACAAG AATTGATACT ATCAGAGAAA AACATGATTT TCCAAGCTGA AAAGTCCAAA 
GCAGAGATAC AGACTTTAAA ATCAGAAATT CAAAGAATGG CCCAAAACCT CCAAGACTTG 
CAGTTAGAAC TTATTAGTAC AAGGTCAGAA AATGAAAATC TCATGAAAGA ATTAAAAAAA 
GAGCAAGAGC GAGTATCTGA CTTAGAAACA ATAAATTCTT CTATTGAAAA CTTACTGAAA 
GATAAAGAGC AAGAAAAAGT ACAGATGAAA GAGGAAGCCA AAATAACAGT GGAGATGCTT 
CAAACTCAAT TAAAGGAGCT AAACGAGACA GTGGTTTCCT TGTGCAATGA CCAAGAGGTC 
TCTAAGACCA AAGAACAGAA TCTGGGTAGT CAAGTACAAA CTCTTGAACT TGAGAAGGCT 
CAGCTGCTAC AGGACCTTGG TGAGGCCAAG AATAAATATA TTATTTTTCA GTCATCTGTA 
AATGCCCTCA CTCAAGAAGT AGAAGCTGGC AAACAGAAAC TAGAGAAGGG GGAGAAAGAG 
ATCAGGACAC TGAAAGAGCA ACTTAAAAGT CAGGAGCAGC TTGTGTGTAA ACTTGCCCAA 
GTGGAAGGAG AGCAGCAACT CTGGCAGAAG CAGAAACTAG AGCTGAGAAA TGTGACTATG 
GCACTGGAGC AGAAGGTCCA AGTGCTGCAA TCTGAAAACA ACACGTTGCA GAGCACCTAT 
GAAGCACTGC AGAATTCCCA CAAGAGTTTA GAGAGTGAAC TTGGATTGAT AAAGTTGGAG 
AAAGTAGCGC TTGTTGAAAG AGTTAGCACA ATATCTGGGA AGGAAGCAGA GCTGCAGAGG 
GAGCTGCGAG ATATGCTACA GAAAACAACA CAGCTGAGCG AAGACTACAA TAAAGAGAAA 
AACAGGCTAA CAGAAGAAGT GGAAGTGCTG CGTGAAGAAC TGCAGAACAC CAAAGCAGCG 
CACCTGAAAT CTGTGAATCA ACTTGAGAAG GAACTTCAGC GTGCTCAGGG GAAAATAAAG 
TTGATGCTCA AATCCTGTAG ACAGCTGGAA GGAGAAAAGG AGATGCTGCA GAAGGAGCTC 
TCCCAGCTTG AAGCTGCACA GCAGCAGAGA GCAGGTTCTC TTGTAGACAG TAACGTAGAT 
GAAGTAATGA CTGAGAACAA AGCGCTGAAA GAGACTCTGG AAGAAAAAGT CAAGGAAGCA 
GATAAGTACT TGGATAAGTA CTGTTCCCTG CTGATAAGCC ACGAGGAGCT CGAGAAAGCC 
AAGGAGATAT TAGAAATAGA AGTTGCTCGG CTGAAGTCAC GGCAGTCCAG ACAGGATCTC 
CAGAGTTCTC CTTTGCTTAA TTCTTCCATT CCAGGACCGT CTCCAAATAC TTCTGTTAGT 
GAGATGAAGT CAGCATCTGG CCAAAATAAA GCTTCAGGCA AGAGGCAAAG GTCCAGTGGG 
ATTTGGGAGC ATGGTAAACG GGCAGCACCT TCTACAGCAG AGACATTTTC TAAGAAAAGC 
AGGAAGTCGG ACAGTAAGAG CACTCGCCCT GCTGAGCACG AGCAGGAAAC CGAGTTTGAG 
CCAGAAGGCC TCCCAGAAGT CGTTAAAAAA GGGTTTGCTG ACATCCCAAC TGGAAAGACA 
AGCCCATATA TCCTTCGGAG AACAACCATG GCAACCAGGA CCAGCCCCCG CTTTGCTACA 
CAGAAGTTAG TGGGATCTTC CCCATCTCTG GGCAAAGAAA ATGTTGTAGA GTCCTCCAAA 
CCAACAGCTG GTGGCAGCAG ATCACAAAAG GTCAAGGTTG TTCAGGAGAG CTCAGCGGAT 
TCACACACTG CCTTCCAAGA ACTCCCAGCA AAATCTCTCA CAGCCAGTAA TATTCCTGGG 
AGAAACTCTA CAGAGAGCCC CAGGGAGGGC CTGAGGGCCA AGCGGGCCTA CCCTGCCTCC 
AGCCCAGCTG CTGGGCCTGA TCCCACGAAC AACGAAAACT GCCGGGTCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cenpf ( XM_017320925.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176414.1 Mus musculus centromere protein F (Cenpf), transcript variant X1, mRNA.

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CloneID	OMu28933
Clone ID Related Accession (Same CDS sequence)	XM_017320925.1 , XM_006497119.2 , XM_006497119.3
Accession Version	XM_017320925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centromere protein F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATAT GCGAACATTT GGAGAAGTCA AGGCAGAAGC TGTCTCATGA ACTTCAAGTT 
AAGGAGTCAC AAGTGAATCT CCAAGAGAGC CAACTGAGCT CATGCAAAAA GCAAATAGAA 
AAACTGGAAC AGGAACTTAA GCGGTGTAAA TCTGAATTTG AAAGAAGCCA ACAAGTTGCA 
CAATCGGCAG ATGTTTCTCT GAATCCATGC AGTACACCAC AGAAACTCTT TGCAACTCCA 
CTCACACCGA GTTCCACATA CGAAGATCTG AAAGAAAAAT ATAATAAAGA AGTTGAAGAA 
CGGAAGAGGT TAGAGGAAGA GGTTAAAGCT TTGCATGCAA AAAAAGTGAG CCTGCCTGTT 
TCCCAAGCCA CCATGAACCA CCGGGACATT GCGAGACATC AGGCTTCCTC ATCAGTGTTT 
CCTTGGCAAC AGGAAAATAC CCCAAGTCGC CTTTCATCGG ATGCTCTGAA AACCCCACTG 
AGGAGAGACG GCTCTGCTGC TCACTTTTTG GGGGAAGAAG TGAGTCCTAA CAAATCAAGT 
ATGAAGACAG GGAGAGGAGA CTGCAGCAGC CTCCCTGGTG AGCCTCACAG CGCTCAGCTT 
TTGCACCAGG CCAAAGCCCA GAATCAAGAC CTAAAAAGCA AGATGACTGA GTTAGAACTA 
CGCCTGCAAG GGCAAGAAAA GGAAATGAGA AGCCAAGTGA ATAAATGTCA AGACTTACAG 
CTACAGCTGG AGAAGACGAA AGTGGAATTG ATTGAAAAGG AGAGAATTTT GAATAAAACC 
AGAGATGAAG TAGTGAGAAG CACAGCACAG TATGACCAGG CCGCAGCCAA GTGTACTACC 
TTGGAACAAA AGCTGAAAAC TTTGACTGAG GAGTTGAGTT GTCACCGACA GAACGCAGAG 
AGTGCTAAAC GTTCTCTGGA ACAGAGGATT AAGGAGAAAG AAAAGGAGCT TCAAGAGGAG 
CTGTCCCGAC AGCATCAATC TTTCCAAGCT CTGGACAGTG AGTACACTCA GATGAAAACC 
AGACTTACCC AGGAGTTACA GCAAGTCAAG CATTTGCACA GCACCCTCCA GCTGGAACTG 
GAGAAGGTCA CATCAGTGAA GCAGCAGTTA GAAAGGAATT TGGAAGAAAT TAGGCTTAAG 
TTGAGCAGAG CAGAACAAGC TCTTCAGGCA AGTCAGGTCG CAGAAAACGA GCTGAGGAGA 
AGCAGTGAGG AAATGAAGAA GGAGAACAGT CTCATTAGGA GTCAGTCTGA GCAGAGGACC 
AGAGAAGTCT GCCACCTGGA GGAAGAACTT GGTAAAGTCA AAGTGTCTTT GAGTAAGAGC 
CAGAATTTTG CAGAAGAAAT GAAGGCTAAG AATACCTCTC AGGAAATCAT GTTACGAGAT 
CTTCAGGAAA AACTAAATCA GCAAGAAAAC TCACTAACTT TAGAGAAGCT GAAACTTGCC 
CTAGCTGATC TGGAAAGACA GCGAAACTGT TCTCAAGATC TCTTGAAGAA AAGGGAACAT 
CACATTGATC AACTGAATAA TAAGTTAAAT AAGATAGAGA AAGAGTTTGA AACTTTGCTG 
AGTGCTTTGG AATTAAAAAA GAAAGAATGT GAAGAATTGA AAGAAGAGAA AAATCAGATT 
TCTTTTTGGA AAATTGATAG TGAAAAACTC ATAAATCAGA TAGAATCAGA AAAAGAAATC 
TTATTGGGTA AAATTAACCA CTTAGAGACC AGCCTCAAGA CACAACAAGT AAGTCCTGAC 
TCTAATGAGA GAATAAGAAC ACTGGAGATG GAAAGAGAAA ACTTTACTGT GGAGATTAAA 
AACCTTCAAA GTATGTTAGA CAGCAAGATG GTAGAGATCA AGACACAGAA ACAAGCTTAC 
TTGGAACTGC AGCAGAAATC CGAATCCTCG GACCAAAAGC ATCAGAAGGA GATAGAGAAT 
ATGTGCTTGA AAGCAAATAA GCTCACTGGG CAAGTTGAAA GTTTGGAATG TAAGCTTCAG 
TTATTGTCAA GTGAAGTAGT GACCAAAGAC CAGCAGTACC AAGACTTGCG TATGGAATAT 
GAGACACTGA GGGATTTGCT CAAGTCCAGA GGATCTTCTC TGGTGACAAA TGAGGATAAT 
CAGCGAAGTT CTGAGGATAA TCAGAGAAGT TCTGAGGATA ATCAGAGAGG CTCTTTGGCT 
TTTGAACAGC AGCCTGCAGT GAGTGATTCC TTTGCAAATG TAATGGGGAG AAAAGGAAGC 
ATAAATTCAG AAAGGAGTGA CTGCTCTGTA GATGGGGGCC GAAGTCCAGA ACATATAGCC 
ATCTTACAAA ATAGAGTCAC TTCACTTGAA AGTTCCTTGG AGTCTCAAAA CCAGATGAAT 
TCAGATTTGC AAATGCGGTG TGAAGAGTTG CTACAAATCA AAGGGGAAGT AGAAGAAAAC 
CTCAGTAAAG CAGAGCAGAT TCATCAGAAT TTTGTGGCTG AAACAAATCA ATGTATTAGT 
AAATTGCAGG AAGATGCTGC AGTTCATCAG AATATTGTTG CTGAGACTTT AGCAACCCTT 
GAGAGTAAGG AAAAAGAGTT ACAGCTTTTG AAAGAAAAAT TAGAAGCTCA GCAAACAGAG 
GTTCAAAAGT TAAATAAGAA TAACTGTCTT CTTGAAGGTA CTCTGAAGGA GCTACAGCTT 
TTATCTGACA CTCTGAGCTC AGAGAAGAAG GAAATGAATT CTATCATCTC ATTAAGTAAA 
AAAAACATTG AAGAGTTAAC CCAAGCAAAC GAGGCTCTCA AGGAAGTTAA TGAGGCCTTA 
GAGCAGGAGA AAATGAATTT ACTCCAAAAG CATGAGAAGA TTACAAGCTG TATAGCAGAA 
CAAGAGAGAA GCATTGCAGA GCTGTCTGAT CAGTACAAGC AAGAAAGACT TCAATTATTA 
CAACGGTGTG AAGAAACAGA AGCTGTGTTG GAAGATCTCA GGGGAAACTA CAAAACAGCA 
CAAGAAAACA ATGCTAAGTT AGAATGCATG CTCAGCGAGT GCACTGCTCT TTGTGAAAAT 
AGAAAAAATG AACTGGAGCA GTTAAAGGAA ACATTTGCAA AGGAACAGCA AGAATTCTTA 
ACAAAATTAG CTTTCGCTGA AGAGCAAAAC AGGAAACTAA TGCTAGAGTT GGAGATAGAG 
CAACAAACTG TGAGATCCGA GATTACAAAC ACCAACAAGC ATTCCATGAG TGCGACTGAT 
GGCTTAAGGC AAGAATGCTT GACTTTAAAC GAAGAGCAAA ATGAGCAGCA AAACGAAGTT 
AGCAACTTAA CACATGAGAA TGAGCAACTG ATGGAGTTAA CACAGACCAA ACATGATTCT 
TATCTCGCAG TAGAGCCAGT TGAGAACTCT GTAAAAGCAA CCGAAGATGA GATAGGTAAG 
AGTAGTTCCC AGTACCAGAT GGATATCGAC ACTAAAGACA TTTCTCTAGA TAGTTATAAG 
GCACAGCTGG TACATCTAGA AGCTTTGGTA AGAATTCTGG AAGTACAGCT TGACCAAAGT 
GAGGAGGAGA ACAAGAAGCT GCATCTGGAA TTACAGACGA TTAGAGAGGA GCTAGAAACC 
AAGAGTTCAC AGGACCCCCA GTCACAGGCA AGGACTGGGC TTAAAGACTG TGACACAGCA 
GAAGAAAAGT ATGTGTCCAT GCTACAGGAG TTGTCAGCAA GTCAAAACGA GAATGCACAC 
TTACAGTGCT CTCTACAGAC AGCAGTGAAC AAACTGAATG AGCTAGGGAA AATGTGTGAC 
GTATTGAGAG TTGAAAAGTT ACAGCTAGAG TCTGAGCTGA ATGACTCACG GACAGAGTGT 
ATCACGGCAA CTAGTCAGAT GACGGCAGAG GTCGAGAAGC TAGTGAGTGA AATGAAAATG 
CTAAACCACG AGAGTGCTCT GTCCCAGAAT GAGCTGATGA AGGACACCTC AGGTGGTGAA 
TTTCATGATA AAGCAAACCA CAGTTCTGTG TTCTTGACTC CTTTGGACAG TAGCAATTTC 
TGTGAACAGA TGACCTTGTC AAGCAAAGAG GTCCGAGTGC ACTTTGCTGA ATTACAGGAG 
AAATTCTCCT GTTTACAAAG TGAACACAAA ATTTTACATG ATCAGCACTG TGAGGTGAGC 
TCTAAGATGT CAGCACTGCG TTCCTACGTG GACACATTAA AAGCTGAAAA TTCTGCCTTG 
TCAATGAGTC TGAGAACCTT GCAGGGTGAC TTGGTAAAGG AGGGGGAGCC TGCAGCTGAG 
GGTGGGCATG GTCTGCCACT GTCGTTCTGT GGGGCAGACA GCCCGTCTCT GACAAATTTT 
GGAGAAACGT CCTTTTACAA AGACGTTTTA GAACAAACTG GAGACACATG TCATCTAAGT 
TTAGAAGGGA ACGCTTCAGC AAATTCTTGT GACTTGGATG AAGAGTTCTC CAGCAGTCTG 
GAGGAGGAGA CTCTGACTGA GAAGGAAAGC CCACCTGCCC CTGGGAGGAC TGTTGAGGGA 
CTTGAAGTCC TATGCCAGGT GTACTTGCAG TCCCTCAAGA ATCTAGAGGA GAAAACTGAG 
AGCCAAAGGA TTATGAAAAA TAAAGAAATT GAAAAGCTTG AGCAGTTACT GAGTTCTGAG 
AGGAAAGAGC TAAGCTGCCT TAGGAAGCAG TATTTGTCAG AAAAGGAGCA ATGGCAGCAG 
AAGCTAACAA GTGTCACTTT GGAAATGGAG TCCAAGTTAG CAGAGGAGAA GCAGCAGACC 
AAGACTCTGT CCCTTGAACT TGAGGTAGCA CGACTTCAGT TACAGGAGCT GGACCTGAGC 
TCCAGGTCTT TGCTTGGCAC TGACTTGGAA AGTGTTGTTC GGTGCCAAAA CGATAATTAT 
GATATAAAAG AATCAGAAGT ATATATTTCA GAGACTACAG AGAAAACACC AAAGCAGGAC 
ACTGACCAAA CTTGTGATAA AGATATTCAG CAGGACCTTG GTCTGGAAAC TTCAGTCACT 
GAGAGTGAGA CTACCAGGCT CACAGGAGAG GGGTGTGAAG AGCAGCCTCC GAAGACCAAT 
TGTGAGGCAC CAGCGGAGGA CAAAACCCAG GACTGCTCAG AATGCATTTC TGAATTGTGT 
TCTAGTTCCA ATGTTTTGGT GCCCATGGAT GTTCTGGAAG ATCAGGGGTC TATCCAGAAT 
CTCCAGTTGC AGAAAGACAC CTTAAATGAG AATTTGAGAT TACTTCCTGA AGTAGAGGAC 
TGGGACAAAA AAGTTGAAAG TTTGCTAAAT GAAATTATGG AGGCAGATTC AAAACTGAGT 
TTACAGGAAG TACAGCTCAA GATGAAGATT GCAACATGCA TACAATTGGA AAAAATAGTC 
AAGGACCTCA GAAAGGAAAA AGCTGACTTA AGTGAAAAGT TGGAATCCCT TCCATGTAAC 
CAGGAGGTAT GTCTGAGAGT AGAAAGGTCA GAAGAAGATC TTGGTTTTAA TTTAGATATG 
GGAGCAAATG AGTTGTTAAG TAAATCTACT AAAGATAATG CAACCAACAC AGAAGACAAT 
TATAAGGAGA AGTTTCTTGA TATGGAAAGA GAACTGACCA GAATTAAGTC TGAGAAAGCT 
AATATTGAGC ATCACATCCT ATCTGTGGAA ACTAACTTAG AGGTGGTTCA AGCAGAGAAG 
CTCTGTTTGG AAAGAGACAC TGAAAGTAAG CAAAAGGTTA TTATTGACCT TAAAGAAGAA 
CTATTTACAG TTATAAGTGA GAGAAACAGA CTTCGGGAAG AATTAGATAA TGTGTCAAAA 
GAAAGCAAAG CACTGGATCA GATGTCTAAA AAGATGAAAG AGAAAATAGA AGAGCTGGAG 
TCTCACCAAA GGGAGAGCCT CCGTCACATT GGGGCAGTAG AGTCTGAGGT CAAGGACAAA 
GCAGATCTTA TTCAGACTCT GTCCTTTAAT GTGGGTGAGC TAACAAAAGA CAAAGCTCAT 
CTCCAGGAGC AGCTGCAGAA TTTGCAGAAT GACTCACAAG AATTATCTTT GGCGATTGGT 
GAGCTGGAAA TACAAATTGG ACAACTGAAT AAAGAGAAAG AATCACTGGT CAAGGAGTCT 
CAGAACTTCC AGATCAAGCT GACTGAGTCA GAGTGTGAAA AGCAGACGAT CTCTAAGGCC 
TTGGAGGTGG CACTCAAGGA GAAAGGTGAG TTTGCAGTGC AGCTGAGCTC AGCCCAGGAG 
GAGGTGCATC AGCTGAGACG AGGCATTGAG AAACTGAGCG TCCGCATTGA GGCCGATGAG 
AAGAAGCACC TCAGTGCTGT GGCGAAGCTG AAAGAAAGCC AGCGTGAAAG CGACTCATTG 
AAGGATACAG TGGAGACTCT GGAGCGGGAA CTGGAGAGGT CAGAAGAAAA CCAAGAGCTG 
GCAATTCTTG ATTCTGAGAA TTTGAAAGCA GAGGTGGAGA CCCTTAAGGC ACAAAAGGAT 
GAAATGACCA AAAGCCTGAG AATTTTCGAA TTAGACCTTG TTACAGTTAG GACTGAAAGA 
GAAAATCTAG CAAAGCAGCT ACAAGAGAAA CAAAGTCGAG TGTCAGAATT AGATGAACGG 
TGCTCTTCCT TGAGAAGACT GTTGGAAGAG AAGGAGCAAG CAAGAGTACA GATGGAAGAA 
GACTCTAAGT CTGCAATGCT GATGCTTCAG ATGCAGTTAA AAGAACTCAG GGAGGAAGTG 
GCAGCCTTGT GTAATGACCA AGAAACCTTG AAGGCCCAAG AACAGAGTCT AGACCAACCA 
GGGGAGGAAG TGCATCATTT GAAAAGTAGC ATTCGAAAGC TCAAAGTTCA CATAGATGCT 
GATGAAAAGA AGCATCAAAA CATCCTAGAA CAACTGAAGG AAAGTAAGCA CCATGCAGAC 
TTGCTTAAGG ACCGAGTTGA GAACCTTGAA CAAGAATTGA TACTATCAGA GAAAAACATG 
ATTTTCCAAG CTGAAAAGTC CAAAGCAGAG ATACAGACTT TAAAATCAGA AATTCAAAGA 
ATGGCCCAAA ACCTCCAAGA CTTGCAGTTA GAACTTATTA GTACAAGGTC AGAAAATGAA 
AATCTCATGA AAGAATTAAA AAAAGAGCAA GAGCGAGTAT CTGACTTAGA AACAATAAAT 
TCTTCTATTG AAAACTTACT GAAAGATAAA GAGCAAGAAA AAGTACAGAT GAAAGAGGAA 
GCCAAAATAA CAGTGGAGAT GCTTCAAACT CAATTAAAGG AGCTAAACGA GACAGTGGTT 
TCCTTGTGCA ATGACCAAGA GGTCTCTAAG ACCAAAGAAC AGAATCTGGG TAGTCAAGTA 
CAAACTCTTG AACTTGAGAA GGCTCAGCTG CTACAGGACC TTGGTGAGGC CAAGAATAAA 
TATATTATTT TTCAGTCATC TGTAAATGCC CTCACTCAAG AAGTAGAAGC TGGCAAACAG 
AAACTAGAGA AGGGGGAGAA AGAGATCAGG ACACTGAAAG AGCAACTTAA AAGTCAGGAG 
CAGCTTGTGT GTAAACTTGC CCAAGTGGAA GGAGAGCAGC AACTCTGGCA GAAGCAGAAA 
CTAGAGCTGA GAAATGTGAC TATGGCACTG GAGCAGAAGG TCCAAGTGCT GCAATCTGAA 
AACAACACGT TGCAGAGCAC CTATGAAGCA CTGCAGAATT CCCACAAGAG TTTAGAGAGT 
GAACTTGGAT TGATAAAGTT GGAGAAAGTA GCGCTTGTTG AAAGAGTTAG CACAATATCT 
GGGAAGGAAG CAGAGCTGCA GAGGGAGCTG CGAGATATGC TACAGAAAAC AACACAGCTG 
AGCGAAGACT ACAATAAAGA GAAAAACAGG CTAACAGAAG AAGTGGAAGT GCTGCGTGAA 
GAACTGCAGA ACACCAAAGC AGCGCACCTG AAATCTGTGA ATCAACTTGA GAAGGAACTT 
CAGCGTGCTC AGGGGAAAAT AAAGTTGATG CTCAAATCCT GTAGACAGCT GGAAGGAGAA 
AAGGAGATGC TGCAGAAGGA GCTCTCCCAG CTTGAAGCTG CACAGCAGCA GAGAGCAGGT 
TCTCTTGTAG ACAGTAACGT AGATGAAGTA ATGACTGAGA ACAAAGCGCT GAAAGAGACT 
CTGGAAGAAA AAGTCAAGGA AGCAGATAAG TACTTGGATA AGTACTGTTC CCTGCTGATA 
AGCCACGAGG AGCTCGAGAA AGCCAAGGAG ATATTAGAAA TAGAAGTTGC TCGGCTGAAG 
TCACGGCAGT CCAGACAGGA TCTCCAGAGT TCTCCTTTGC TTAATTCTTC CATTCCAGGA 
CCGTCTCCAA ATACTTCTGT TAGTGAGATG AAGTCAGCAT CTGGCCAAAA TAAAGCTTCA 
GGCAAGAGGC AAAGGTCCAG TGGGATTTGG GAGCATGGTA AACGGGCAGC ACCTTCTACA 
GCAGAGACAT TTTCTAAGAA AAGCAGGAAG TCGGACAGTA AGAGCACTCG CCCTGCTGAG 
CACGAGCAGG AAACCGAGTT TGAGCCAGAA GGCCTCCCAG AAGTCGTTAA AAAAGGGTTT 
GCTGACATCC CAACTGGAAA GACAAGCCCA TATATCCTTC GGAGAACAAC CATGGCAACC 
AGGACCAGCC CCCGCTTTGC TACACAGAAG TTAGTGGGAT CTTCCCCATC TCTGGGCAAA 
GAAAATGTTG TAGAGTCCTC CAAACCAACA GCTGGTGGCA GCAGATCACA AAAGGTCAAG 
GTTGTTCAGG AGAGCTCAGC GGATTCACAC ACTGCCTTCC AAGAACTCCC AGCAAAATCT 
CTCACAGCCA GTAATATTCC TGGGAGAAAC TCTACAGAGA GCCCCAGGGA GGGCCTGAGG 
GCCAAGCGGG CCTACCCTGC CTCCAGCCCA GCTGCTGGGC CTGATCCCAC GAACAACGAA 
AACTGCCGGG TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176414.1
CDS	444..9221
Translation	MEICEHLEKSRQKLSHELQVKESQVNLQESQLSSCKKQIEKLEQELKRCKSEFERSQQVAQSADVSLNPCSTPQKLFATPLTPSSTYEDLKEKYNKEVEERKRLEEEVKALHAKKVSLPVSQATMNHRDIARHQASSSVFPWQQENTPSRLSSDALKTPLRRDGSAAHFLGEEVSPNKSSMKTGRGDCSSLPGEPHSAQLLHQAKAQNQDLKSKMTELELRLQGQEKEMRSQVNKCQDLQLQLEKTKVELIEKERILNKTRDEVVRSTAQYDQAAAKCTTLEQKLKTLTEELSCHRQNAESAKRSLEQRIKEKEKELQEELSRQHQSFQALDSEYTQMKTRLTQELQQVKHLHSTLQLELEKVTSVKQQLERNLEEIRLKLSRAEQALQASQVAENELRRSSEEMKKENSLIRSQSEQRTREVCHLEEELGKVKVSLSKSQNFAEEMKAKNTSQEIMLRDLQEKLNQQENSLTLEKLKLALADLERQRNCSQDLLKKREHHIDQLNNKLNKIEKEFETLLSALELKKKECEELKEEKNQISFWKIDSEKLINQIESEKEILLGKINHLETSLKTQQVSPDSNERIRTLEMERENFTVEIKNLQSMLDSKMVEIKTQKQAYLELQQKSESSDQKHQKEIENMCLKANKLTGQVESLECKLQLLSSEVVTKDQQYQDLRMEYETLRDLLKSRGSSLVTNEDNQRSSEDNQRSSEDNQRGSLAFEQQPAVSDSFANVMGRKGSINSERSDCSVDGGRSPEHIAILQNRVTSLESSLESQNQMNSDLQMRCEELLQIKGEVEENLSKAEQIHQNFVAETNQCISKLQEDAAVHQNIVAETLATLESKEKELQLLKEKLEAQQTEVQKLNKNNCLLEGTLKELQLLSDTLSSEKKEMNSIISLSKKNIEELTQANEALKEVNEALEQEKMNLLQKHEKITSCIAEQERSIAELSDQYKQERLQLLQRCEETEAVLEDLRGNYKTAQENNAKLECMLSECTALCENRKNELEQLKETFAKEQQEFLTKLAFAEEQNRKLMLELEIEQQTVRSEITNTNKHSMSATDGLRQECLTLNEEQNEQQNEVSNLTHENEQLMELTQTKHDSYLAVEPVENSVKATEDEIGKSSSQYQMDIDTKDISLDSYKAQLVHLEALVRILEVQLDQSEEENKKLHLELQTIREELETKSSQDPQSQARTGLKDCDTAEEKYVSMLQELSASQNENAHLQCSLQTAVNKLNELGKMCDVLRVEKLQLESELNDSRTECITATSQMTAEVEKLVSEMKMLNHESALSQNELMKDTSGGEFHDKANHSSVFLTPLDSSNFCEQMTLSSKEVRVHFAELQEKFSCLQSEHKILHDQHCEVSSKMSALRSYVDTLKAENSALSMSLRTLQGDLVKEGEPAAEGGHGLPLSFCGADSPSLTNFGETSFYKDVLEQTGDTCHLSLEGNASANSCDLDEEFSSSLEEETLTEKESPPAPGRTVEGLEVLCQVYLQSLKNLEEKTESQRIMKNKEIEKLEQLLSSERKELSCLRKQYLSEKEQWQQKLTSVTLEMESKLAEEKQQTKTLSLELEVARLQLQELDLSSRSLLGTDLESVVRCQNDNYDIKESEVYISETTEKTPKQDTDQTCDKDIQQDLGLETSVTESETTRLTGEGCEEQPPKTNCEAPAEDKTQDCSECISELCSSSNVLVPMDVLEDQGSIQNLQLQKDTLNENLRLLPEVEDWDKKVESLLNEIMEADSKLSLQEVQLKMKIATCIQLEKIVKDLRKEKADLSEKLESLPCNQEVCLRVERSEEDLGFNLDMGANELLSKSTKDNATNTEDNYKEKFLDMERELTRIKSEKANIEHHILSVETNLEVVQAEKLCLERDTESKQKVIIDLKEELFTVISERNRLREELDNVSKESKALDQMSKKMKEKIEELESHQRESLRHIGAVESEVKDKADLIQTLSFNVGELTKDKAHLQEQLQNLQNDSQELSLAIGELEIQIGQLNKEKESLVKESQNFQIKLTESECEKQTISKALEVALKEKGEFAVQLSSAQEEVHQLRRGIEKLSVRIEADEKKHLSAVAKLKESQRESDSLKDTVETLERELERSEENQELAILDSENLKAEVETLKAQKDEMTKSLRIFELDLVTVRTERENLAKQLQEKQSRVSELDERCSSLRRLLEEKEQARVQMEEDSKSAMLMLQMQLKELREEVAALCNDQETLKAQEQSLDQPGEEVHHLKSSIRKLKVHIDADEKKHQNILEQLKESKHHADLLKDRVENLEQELILSEKNMIFQAEKSKAEIQTLKSEIQRMAQNLQDLQLELISTRSENENLMKELKKEQERVSDLETINSSIENLLKDKEQEKVQMKEEAKITVEMLQTQLKELNETVVSLCNDQEVSKTKEQNLGSQVQTLELEKAQLLQDLGEAKNKYIIFQSSVNALTQEVEAGKQKLEKGEKEIRTLKEQLKSQEQLVCKLAQVEGEQQLWQKQKLELRNVTMALEQKVQVLQSENNTLQSTYEALQNSHKSLESELGLIKLEKVALVERVSTISGKEAELQRELRDMLQKTTQLSEDYNKEKNRLTEEVEVLREELQNTKAAHLKSVNQLEKELQRAQGKIKLMLKSCRQLEGEKEMLQKELSQLEAAQQQRAGSLVDSNVDEVMTENKALKETLEEKVKEADKYLDKYCSLLISHEELEKAKEILEIEVARLKSRQSRQDLQSSPLLNSSIPGPSPNTSVSEMKSASGQNKASGKRQRSSGIWEHGKRAAPSTAETFSKKSRKSDSKSTRPAEHEQETEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRFATQKLVGSSPSLGKENVVESSKPTAGGSRSQKVKVVQESSADSHTAFQELPAKSLTASNIPGRNSTESPREGLRAKRAYPASSPAAGPDPTNNENCRVQ

Target ORF information:

RefSeq Version	XM_017320925.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centromere protein F (Cenpf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320925.1

ORF Insert Sequence:

ATGGAGATAT GCGAACATTT GGAGAAGTCA AGGCAGAAGC TGTCTCATGA ACTTCAAGTT 
AAGGAGTCAC AAGTGAATCT CCAAGAGAGC CAACTGAGCT CATGCAAAAA GCAAATAGAA 
AAACTGGAAC AGGAACTTAA GCGGTGTAAA TCTGAATTTG AAAGAAGCCA ACAAGTTGCA 
CAATCGGCAG ATGTTTCTCT GAATCCATGC AGTACACCAC AGAAACTCTT TGCAACTCCA 
CTCACACCGA GTTCCACATA CGAAGATCTG AAAGAAAAAT ATAATAAAGA AGTTGAAGAA 
CGGAAGAGGT TAGAGGAAGA GGTTAAAGCT TTGCATGCAA AAAAAGTGAG CCTGCCTGTT 
TCCCAAGCCA CCATGAACCA CCGGGACATT GCGAGACATC AGGCTTCCTC ATCAGTGTTT 
CCTTGGCAAC AGGAAAATAC CCCAAGTCGC CTTTCATCGG ATGCTCTGAA AACCCCACTG 
AGGAGAGACG GCTCTGCTGC TCACTTTTTG GGGGAAGAAG TGAGTCCTAA CAAATCAAGT 
ATGAAGACAG GGAGAGGAGA CTGCAGCAGC CTCCCTGGTG AGCCTCACAG CGCTCAGCTT 
TTGCACCAGG CCAAAGCCCA GAATCAAGAC CTAAAAAGCA AGATGACTGA GTTAGAACTA 
CGCCTGCAAG GGCAAGAAAA GGAAATGAGA AGCCAAGTGA ATAAATGTCA AGACTTACAG 
CTACAGCTGG AGAAGACGAA AGTGGAATTG ATTGAAAAGG AGAGAATTTT GAATAAAACC 
AGAGATGAAG TAGTGAGAAG CACAGCACAG TATGACCAGG CCGCAGCCAA GTGTACTACC 
TTGGAACAAA AGCTGAAAAC TTTGACTGAG GAGTTGAGTT GTCACCGACA GAACGCAGAG 
AGTGCTAAAC GTTCTCTGGA ACAGAGGATT AAGGAGAAAG AAAAGGAGCT TCAAGAGGAG 
CTGTCCCGAC AGCATCAATC TTTCCAAGCT CTGGACAGTG AGTACACTCA GATGAAAACC 
AGACTTACCC AGGAGTTACA GCAAGTCAAG CATTTGCACA GCACCCTCCA GCTGGAACTG 
GAGAAGGTCA CATCAGTGAA GCAGCAGTTA GAAAGGAATT TGGAAGAAAT TAGGCTTAAG 
TTGAGCAGAG CAGAACAAGC TCTTCAGGCA AGTCAGGTCG CAGAAAACGA GCTGAGGAGA 
AGCAGTGAGG AAATGAAGAA GGAGAACAGT CTCATTAGGA GTCAGTCTGA GCAGAGGACC 
AGAGAAGTCT GCCACCTGGA GGAAGAACTT GGTAAAGTCA AAGTGTCTTT GAGTAAGAGC 
CAGAATTTTG CAGAAGAAAT GAAGGCTAAG AATACCTCTC AGGAAATCAT GTTACGAGAT 
CTTCAGGAAA AACTAAATCA GCAAGAAAAC TCACTAACTT TAGAGAAGCT GAAACTTGCC 
CTAGCTGATC TGGAAAGACA GCGAAACTGT TCTCAAGATC TCTTGAAGAA AAGGGAACAT 
CACATTGATC AACTGAATAA TAAGTTAAAT AAGATAGAGA AAGAGTTTGA AACTTTGCTG 
AGTGCTTTGG AATTAAAAAA GAAAGAATGT GAAGAATTGA AAGAAGAGAA AAATCAGATT 
TCTTTTTGGA AAATTGATAG TGAAAAACTC ATAAATCAGA TAGAATCAGA AAAAGAAATC 
TTATTGGGTA AAATTAACCA CTTAGAGACC AGCCTCAAGA CACAACAAGT AAGTCCTGAC 
TCTAATGAGA GAATAAGAAC ACTGGAGATG GAAAGAGAAA ACTTTACTGT GGAGATTAAA 
AACCTTCAAA GTATGTTAGA CAGCAAGATG GTAGAGATCA AGACACAGAA ACAAGCTTAC 
TTGGAACTGC AGCAGAAATC CGAATCCTCG GACCAAAAGC ATCAGAAGGA GATAGAGAAT 
ATGTGCTTGA AAGCAAATAA GCTCACTGGG CAAGTTGAAA GTTTGGAATG TAAGCTTCAG 
TTATTGTCAA GTGAAGTAGT GACCAAAGAC CAGCAGTACC AAGACTTGCG TATGGAATAT 
GAGACACTGA GGGATTTGCT CAAGTCCAGA GGATCTTCTC TGGTGACAAA TGAGGATAAT 
CAGCGAAGTT CTGAGGATAA TCAGAGAAGT TCTGAGGATA ATCAGAGAGG CTCTTTGGCT 
TTTGAACAGC AGCCTGCAGT GAGTGATTCC TTTGCAAATG TAATGGGGAG AAAAGGAAGC 
ATAAATTCAG AAAGGAGTGA CTGCTCTGTA GATGGGGGCC GAAGTCCAGA ACATATAGCC 
ATCTTACAAA ATAGAGTCAC TTCACTTGAA AGTTCCTTGG AGTCTCAAAA CCAGATGAAT 
TCAGATTTGC AAATGCGGTG TGAAGAGTTG CTACAAATCA AAGGGGAAGT AGAAGAAAAC 
CTCAGTAAAG CAGAGCAGAT TCATCAGAAT TTTGTGGCTG AAACAAATCA ATGTATTAGT 
AAATTGCAGG AAGATGCTGC AGTTCATCAG AATATTGTTG CTGAGACTTT AGCAACCCTT 
GAGAGTAAGG AAAAAGAGTT ACAGCTTTTG AAAGAAAAAT TAGAAGCTCA GCAAACAGAG 
GTTCAAAAGT TAAATAAGAA TAACTGTCTT CTTGAAGGTA CTCTGAAGGA GCTACAGCTT 
TTATCTGACA CTCTGAGCTC AGAGAAGAAG GAAATGAATT CTATCATCTC ATTAAGTAAA 
AAAAACATTG AAGAGTTAAC CCAAGCAAAC GAGGCTCTCA AGGAAGTTAA TGAGGCCTTA 
GAGCAGGAGA AAATGAATTT ACTCCAAAAG CATGAGAAGA TTACAAGCTG TATAGCAGAA 
CAAGAGAGAA GCATTGCAGA GCTGTCTGAT CAGTACAAGC AAGAAAGACT TCAATTATTA 
CAACGGTGTG AAGAAACAGA AGCTGTGTTG GAAGATCTCA GGGGAAACTA CAAAACAGCA 
CAAGAAAACA ATGCTAAGTT AGAATGCATG CTCAGCGAGT GCACTGCTCT TTGTGAAAAT 
AGAAAAAATG AACTGGAGCA GTTAAAGGAA ACATTTGCAA AGGAACAGCA AGAATTCTTA 
ACAAAATTAG CTTTCGCTGA AGAGCAAAAC AGGAAACTAA TGCTAGAGTT GGAGATAGAG 
CAACAAACTG TGAGATCCGA GATTACAAAC ACCAACAAGC ATTCCATGAG TGCGACTGAT 
GGCTTAAGGC AAGAATGCTT GACTTTAAAC GAAGAGCAAA ATGAGCAGCA AAACGAAGTT 
AGCAACTTAA CACATGAGAA TGAGCAACTG ATGGAGTTAA CACAGACCAA ACATGATTCT 
TATCTCGCAG TAGAGCCAGT TGAGAACTCT GTAAAAGCAA CCGAAGATGA GATAGGTAAG 
AGTAGTTCCC AGTACCAGAT GGATATCGAC ACTAAAGACA TTTCTCTAGA TAGTTATAAG 
GCACAGCTGG TACATCTAGA AGCTTTGGTA AGAATTCTGG AAGTACAGCT TGACCAAAGT 
GAGGAGGAGA ACAAGAAGCT GCATCTGGAA TTACAGACGA TTAGAGAGGA GCTAGAAACC 
AAGAGTTCAC AGGACCCCCA GTCACAGGCA AGGACTGGGC TTAAAGACTG TGACACAGCA 
GAAGAAAAGT ATGTGTCCAT GCTACAGGAG TTGTCAGCAA GTCAAAACGA GAATGCACAC 
TTACAGTGCT CTCTACAGAC AGCAGTGAAC AAACTGAATG AGCTAGGGAA AATGTGTGAC 
GTATTGAGAG TTGAAAAGTT ACAGCTAGAG TCTGAGCTGA ATGACTCACG GACAGAGTGT 
ATCACGGCAA CTAGTCAGAT GACGGCAGAG GTCGAGAAGC TAGTGAGTGA AATGAAAATG 
CTAAACCACG AGAGTGCTCT GTCCCAGAAT GAGCTGATGA AGGACACCTC AGGTGGTGAA 
TTTCATGATA AAGCAAACCA CAGTTCTGTG TTCTTGACTC CTTTGGACAG TAGCAATTTC 
TGTGAACAGA TGACCTTGTC AAGCAAAGAG GTCCGAGTGC ACTTTGCTGA ATTACAGGAG 
AAATTCTCCT GTTTACAAAG TGAACACAAA ATTTTACATG ATCAGCACTG TGAGGTGAGC 
TCTAAGATGT CAGCACTGCG TTCCTACGTG GACACATTAA AAGCTGAAAA TTCTGCCTTG 
TCAATGAGTC TGAGAACCTT GCAGGGTGAC TTGGTAAAGG AGGGGGAGCC TGCAGCTGAG 
GGTGGGCATG GTCTGCCACT GTCGTTCTGT GGGGCAGACA GCCCGTCTCT GACAAATTTT 
GGAGAAACGT CCTTTTACAA AGACGTTTTA GAACAAACTG GAGACACATG TCATCTAAGT 
TTAGAAGGGA ACGCTTCAGC AAATTCTTGT GACTTGGATG AAGAGTTCTC CAGCAGTCTG 
GAGGAGGAGA CTCTGACTGA GAAGGAAAGC CCACCTGCCC CTGGGAGGAC TGTTGAGGGA 
CTTGAAGTCC TATGCCAGGT GTACTTGCAG TCCCTCAAGA ATCTAGAGGA GAAAACTGAG 
AGCCAAAGGA TTATGAAAAA TAAAGAAATT GAAAAGCTTG AGCAGTTACT GAGTTCTGAG 
AGGAAAGAGC TAAGCTGCCT TAGGAAGCAG TATTTGTCAG AAAAGGAGCA ATGGCAGCAG 
AAGCTAACAA GTGTCACTTT GGAAATGGAG TCCAAGTTAG CAGAGGAGAA GCAGCAGACC 
AAGACTCTGT CCCTTGAACT TGAGGTAGCA CGACTTCAGT TACAGGAGCT GGACCTGAGC 
TCCAGGTCTT TGCTTGGCAC TGACTTGGAA AGTGTTGTTC GGTGCCAAAA CGATAATTAT 
GATATAAAAG AATCAGAAGT ATATATTTCA GAGACTACAG AGAAAACACC AAAGCAGGAC 
ACTGACCAAA CTTGTGATAA AGATATTCAG CAGGACCTTG GTCTGGAAAC TTCAGTCACT 
GAGAGTGAGA CTACCAGGCT CACAGGAGAG GGGTGTGAAG AGCAGCCTCC GAAGACCAAT 
TGTGAGGCAC CAGCGGAGGA CAAAACCCAG GACTGCTCAG AATGCATTTC TGAATTGTGT 
TCTAGTTCCA ATGTTTTGGT GCCCATGGAT GTTCTGGAAG ATCAGGGGTC TATCCAGAAT 
CTCCAGTTGC AGAAAGACAC CTTAAATGAG AATTTGAGAT TACTTCCTGA AGTAGAGGAC 
TGGGACAAAA AAGTTGAAAG TTTGCTAAAT GAAATTATGG AGGCAGATTC AAAACTGAGT 
TTACAGGAAG TACAGCTCAA GATGAAGATT GCAACATGCA TACAATTGGA AAAAATAGTC 
AAGGACCTCA GAAAGGAAAA AGCTGACTTA AGTGAAAAGT TGGAATCCCT TCCATGTAAC 
CAGGAGGTAT GTCTGAGAGT AGAAAGGTCA GAAGAAGATC TTGGTTTTAA TTTAGATATG 
GGAGCAAATG AGTTGTTAAG TAAATCTACT AAAGATAATG CAACCAACAC AGAAGACAAT 
TATAAGGAGA AGTTTCTTGA TATGGAAAGA GAACTGACCA GAATTAAGTC TGAGAAAGCT 
AATATTGAGC ATCACATCCT ATCTGTGGAA ACTAACTTAG AGGTGGTTCA AGCAGAGAAG 
CTCTGTTTGG AAAGAGACAC TGAAAGTAAG CAAAAGGTTA TTATTGACCT TAAAGAAGAA 
CTATTTACAG TTATAAGTGA GAGAAACAGA CTTCGGGAAG AATTAGATAA TGTGTCAAAA 
GAAAGCAAAG CACTGGATCA GATGTCTAAA AAGATGAAAG AGAAAATAGA AGAGCTGGAG 
TCTCACCAAA GGGAGAGCCT CCGTCACATT GGGGCAGTAG AGTCTGAGGT CAAGGACAAA 
GCAGATCTTA TTCAGACTCT GTCCTTTAAT GTGGGTGAGC TAACAAAAGA CAAAGCTCAT 
CTCCAGGAGC AGCTGCAGAA TTTGCAGAAT GACTCACAAG AATTATCTTT GGCGATTGGT 
GAGCTGGAAA TACAAATTGG ACAACTGAAT AAAGAGAAAG AATCACTGGT CAAGGAGTCT 
CAGAACTTCC AGATCAAGCT GACTGAGTCA GAGTGTGAAA AGCAGACGAT CTCTAAGGCC 
TTGGAGGTGG CACTCAAGGA GAAAGGTGAG TTTGCAGTGC AGCTGAGCTC AGCCCAGGAG 
GAGGTGCATC AGCTGAGACG AGGCATTGAG AAACTGAGCG TCCGCATTGA GGCCGATGAG 
AAGAAGCACC TCAGTGCTGT GGCGAAGCTG AAAGAAAGCC AGCGTGAAAG CGACTCATTG 
AAGGATACAG TGGAGACTCT GGAGCGGGAA CTGGAGAGGT CAGAAGAAAA CCAAGAGCTG 
GCAATTCTTG ATTCTGAGAA TTTGAAAGCA GAGGTGGAGA CCCTTAAGGC ACAAAAGGAT 
GAAATGACCA AAAGCCTGAG AATTTTCGAA TTAGACCTTG TTACAGTTAG GACTGAAAGA 
GAAAATCTAG CAAAGCAGCT ACAAGAGAAA CAAAGTCGAG TGTCAGAATT AGATGAACGG 
TGCTCTTCCT TGAGAAGACT GTTGGAAGAG AAGGAGCAAG CAAGAGTACA GATGGAAGAA 
GACTCTAAGT CTGCAATGCT GATGCTTCAG ATGCAGTTAA AAGAACTCAG GGAGGAAGTG 
GCAGCCTTGT GTAATGACCA AGAAACCTTG AAGGCCCAAG AACAGAGTCT AGACCAACCA 
GGGGAGGAAG TGCATCATTT GAAAAGTAGC ATTCGAAAGC TCAAAGTTCA CATAGATGCT 
GATGAAAAGA AGCATCAAAA CATCCTAGAA CAACTGAAGG AAAGTAAGCA CCATGCAGAC 
TTGCTTAAGG ACCGAGTTGA GAACCTTGAA CAAGAATTGA TACTATCAGA GAAAAACATG 
ATTTTCCAAG CTGAAAAGTC CAAAGCAGAG ATACAGACTT TAAAATCAGA AATTCAAAGA 
ATGGCCCAAA ACCTCCAAGA CTTGCAGTTA GAACTTATTA GTACAAGGTC AGAAAATGAA 
AATCTCATGA AAGAATTAAA AAAAGAGCAA GAGCGAGTAT CTGACTTAGA AACAATAAAT 
TCTTCTATTG AAAACTTACT GAAAGATAAA GAGCAAGAAA AAGTACAGAT GAAAGAGGAA 
GCCAAAATAA CAGTGGAGAT GCTTCAAACT CAATTAAAGG AGCTAAACGA GACAGTGGTT 
TCCTTGTGCA ATGACCAAGA GGTCTCTAAG ACCAAAGAAC AGAATCTGGG TAGTCAAGTA 
CAAACTCTTG AACTTGAGAA GGCTCAGCTG CTACAGGACC TTGGTGAGGC CAAGAATAAA 
TATATTATTT TTCAGTCATC TGTAAATGCC CTCACTCAAG AAGTAGAAGC TGGCAAACAG 
AAACTAGAGA AGGGGGAGAA AGAGATCAGG ACACTGAAAG AGCAACTTAA AAGTCAGGAG 
CAGCTTGTGT GTAAACTTGC CCAAGTGGAA GGAGAGCAGC AACTCTGGCA GAAGCAGAAA 
CTAGAGCTGA GAAATGTGAC TATGGCACTG GAGCAGAAGG TCCAAGTGCT GCAATCTGAA 
AACAACACGT TGCAGAGCAC CTATGAAGCA CTGCAGAATT CCCACAAGAG TTTAGAGAGT 
GAACTTGGAT TGATAAAGTT GGAGAAAGTA GCGCTTGTTG AAAGAGTTAG CACAATATCT 
GGGAAGGAAG CAGAGCTGCA GAGGGAGCTG CGAGATATGC TACAGAAAAC AACACAGCTG 
AGCGAAGACT ACAATAAAGA GAAAAACAGG CTAACAGAAG AAGTGGAAGT GCTGCGTGAA 
GAACTGCAGA ACACCAAAGC AGCGCACCTG AAATCTGTGA ATCAACTTGA GAAGGAACTT 
CAGCGTGCTC AGGGGAAAAT AAAGTTGATG CTCAAATCCT GTAGACAGCT GGAAGGAGAA 
AAGGAGATGC TGCAGAAGGA GCTCTCCCAG CTTGAAGCTG CACAGCAGCA GAGAGCAGGT 
TCTCTTGTAG ACAGTAACGT AGATGAAGTA ATGACTGAGA ACAAAGCGCT GAAAGAGACT 
CTGGAAGAAA AAGTCAAGGA AGCAGATAAG TACTTGGATA AGTACTGTTC CCTGCTGATA 
AGCCACGAGG AGCTCGAGAA AGCCAAGGAG ATATTAGAAA TAGAAGTTGC TCGGCTGAAG 
TCACGGCAGT CCAGACAGGA TCTCCAGAGT TCTCCTTTGC TTAATTCTTC CATTCCAGGA 
CCGTCTCCAA ATACTTCTGT TAGTGAGATG AAGTCAGCAT CTGGCCAAAA TAAAGCTTCA 
GGCAAGAGGC AAAGGTCCAG TGGGATTTGG GAGCATGGTA AACGGGCAGC ACCTTCTACA 
GCAGAGACAT TTTCTAAGAA AAGCAGGAAG TCGGACAGTA AGAGCACTCG CCCTGCTGAG 
CACGAGCAGG AAACCGAGTT TGAGCCAGAA GGCCTCCCAG AAGTCGTTAA AAAAGGGTTT 
GCTGACATCC CAACTGGAAA GACAAGCCCA TATATCCTTC GGAGAACAAC CATGGCAACC 
AGGACCAGCC CCCGCTTTGC TACACAGAAG TTAGTGGGAT CTTCCCCATC TCTGGGCAAA 
GAAAATGTTG TAGAGTCCTC CAAACCAACA GCTGGTGGCA GCAGATCACA AAAGGTCAAG 
GTTGTTCAGG AGAGCTCAGC GGATTCACAC ACTGCCTTCC AAGAACTCCC AGCAAAATCT 
CTCACAGCCA GTAATATTCC TGGGAGAAAC TCTACAGAGA GCCCCAGGGA GGGCCTGAGG 
GCCAAGCGGG CCTACCCTGC CTCCAGCCCA GCTGCTGGGC CTGATCCCAC GAACAACGAA 
AACTGCCGGG TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cenpf ( XM_006497119.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497182.1 Mus musculus centromere protein F (Cenpf), transcript variant X2, mRNA.

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CloneID	OMu28933
Clone ID Related Accession (Same CDS sequence)	XM_017320925.1 , XM_006497119.2 , XM_006497119.3
Accession Version	XM_006497119.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centromere protein F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006497119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATAT GCGAACATTT GGAGAAGTCA AGGCAGAAGC TGTCTCATGA ACTTCAAGTT 
AAGGAGTCAC AAGTGAATCT CCAAGAGAGC CAACTGAGCT CATGCAAAAA GCAAATAGAA 
AAACTGGAAC AGGAACTTAA GCGGTGTAAA TCTGAATTTG AAAGAAGCCA ACAAGTTGCA 
CAATCGGCAG ATGTTTCTCT GAATCCATGC AGTACACCAC AGAAACTCTT TGCAACTCCA 
CTCACACCGA GTTCCACATA CGAAGATCTG AAAGAAAAAT ATAATAAAGA AGTTGAAGAA 
CGGAAGAGGT TAGAGGAAGA GGTTAAAGCT TTGCATGCAA AAAAAGTGAG CCTGCCTGTT 
TCCCAAGCCA CCATGAACCA CCGGGACATT GCGAGACATC AGGCTTCCTC ATCAGTGTTT 
CCTTGGCAAC AGGAAAATAC CCCAAGTCGC CTTTCATCGG ATGCTCTGAA AACCCCACTG 
AGGAGAGACG GCTCTGCTGC TCACTTTTTG GGGGAAGAAG TGAGTCCTAA CAAATCAAGT 
ATGAAGACAG GGAGAGGAGA CTGCAGCAGC CTCCCTGGTG AGCCTCACAG CGCTCAGCTT 
TTGCACCAGG CCAAAGCCCA GAATCAAGAC CTAAAAAGCA AGATGACTGA GTTAGAACTA 
CGCCTGCAAG GGCAAGAAAA GGAAATGAGA AGCCAAGTGA ATAAATGTCA AGACTTACAG 
CTACAGCTGG AGAAGACGAA AGTGGAATTG ATTGAAAAGG AGAGAATTTT GAATAAAACC 
AGAGATGAAG TAGTGAGAAG CACAGCACAG TATGACCAGG CCGCAGCCAA GTGTACTACC 
TTGGAACAAA AGCTGAAAAC TTTGACTGAG GAGTTGAGTT GTCACCGACA GAACGCAGAG 
AGTGCTAAAC GTTCTCTGGA ACAGAGGATT AAGGAGAAAG AAAAGGAGCT TCAAGAGGAG 
CTGTCCCGAC AGCATCAATC TTTCCAAGCT CTGGACAGTG AGTACACTCA GATGAAAACC 
AGACTTACCC AGGAGTTACA GCAAGTCAAG CATTTGCACA GCACCCTCCA GCTGGAACTG 
GAGAAGGTCA CATCAGTGAA GCAGCAGTTA GAAAGGAATT TGGAAGAAAT TAGGCTTAAG 
TTGAGCAGAG CAGAACAAGC TCTTCAGGCA AGTCAGGTCG CAGAAAACGA GCTGAGGAGA 
AGCAGTGAGG AAATGAAGAA GGAGAACAGT CTCATTAGGA GTCAGTCTGA GCAGAGGACC 
AGAGAAGTCT GCCACCTGGA GGAAGAACTT GGTAAAGTCA AAGTGTCTTT GAGTAAGAGC 
CAGAATTTTG CAGAAGAAAT GAAGGCTAAG AATACCTCTC AGGAAATCAT GTTACGAGAT 
CTTCAGGAAA AACTAAATCA GCAAGAAAAC TCACTAACTT TAGAGAAGCT GAAACTTGCC 
CTAGCTGATC TGGAAAGACA GCGAAACTGT TCTCAAGATC TCTTGAAGAA AAGGGAACAT 
CACATTGATC AACTGAATAA TAAGTTAAAT AAGATAGAGA AAGAGTTTGA AACTTTGCTG 
AGTGCTTTGG AATTAAAAAA GAAAGAATGT GAAGAATTGA AAGAAGAGAA AAATCAGATT 
TCTTTTTGGA AAATTGATAG TGAAAAACTC ATAAATCAGA TAGAATCAGA AAAAGAAATC 
TTATTGGGTA AAATTAACCA CTTAGAGACC AGCCTCAAGA CACAACAAGT AAGTCCTGAC 
TCTAATGAGA GAATAAGAAC ACTGGAGATG GAAAGAGAAA ACTTTACTGT GGAGATTAAA 
AACCTTCAAA GTATGTTAGA CAGCAAGATG GTAGAGATCA AGACACAGAA ACAAGCTTAC 
TTGGAACTGC AGCAGAAATC CGAATCCTCG GACCAAAAGC ATCAGAAGGA GATAGAGAAT 
ATGTGCTTGA AAGCAAATAA GCTCACTGGG CAAGTTGAAA GTTTGGAATG TAAGCTTCAG 
TTATTGTCAA GTGAAGTAGT GACCAAAGAC CAGCAGTACC AAGACTTGCG TATGGAATAT 
GAGACACTGA GGGATTTGCT CAAGTCCAGA GGATCTTCTC TGGTGACAAA TGAGGATAAT 
CAGCGAAGTT CTGAGGATAA TCAGAGAAGT TCTGAGGATA ATCAGAGAGG CTCTTTGGCT 
TTTGAACAGC AGCCTGCAGT GAGTGATTCC TTTGCAAATG TAATGGGGAG AAAAGGAAGC 
ATAAATTCAG AAAGGAGTGA CTGCTCTGTA GATGGGGGCC GAAGTCCAGA ACATATAGCC 
ATCTTACAAA ATAGAGTCAC TTCACTTGAA AGTTCCTTGG AGTCTCAAAA CCAGATGAAT 
TCAGATTTGC AAATGCGGTG TGAAGAGTTG CTACAAATCA AAGGGGAAGT AGAAGAAAAC 
CTCAGTAAAG CAGAGCAGAT TCATCAGAAT TTTGTGGCTG AAACAAATCA ATGTATTAGT 
AAATTGCAGG AAGATGCTGC AGTTCATCAG AATATTGTTG CTGAGACTTT AGCAACCCTT 
GAGAGTAAGG AAAAAGAGTT ACAGCTTTTG AAAGAAAAAT TAGAAGCTCA GCAAACAGAG 
GTTCAAAAGT TAAATAAGAA TAACTGTCTT CTTGAAGGTA CTCTGAAGGA GCTACAGCTT 
TTATCTGACA CTCTGAGCTC AGAGAAGAAG GAAATGAATT CTATCATCTC ATTAAGTAAA 
AAAAACATTG AAGAGTTAAC CCAAGCAAAC GAGGCTCTCA AGGAAGTTAA TGAGGCCTTA 
GAGCAGGAGA AAATGAATTT ACTCCAAAAG CATGAGAAGA TTACAAGCTG TATAGCAGAA 
CAAGAGAGAA GCATTGCAGA GCTGTCTGAT CAGTACAAGC AAGAAAGACT TCAATTATTA 
CAACGGTGTG AAGAAACAGA AGCTGTGTTG GAAGATCTCA GGGGAAACTA CAAAACAGCA 
CAAGAAAACA ATGCTAAGTT AGAATGCATG CTCAGCGAGT GCACTGCTCT TTGTGAAAAT 
AGAAAAAATG AACTGGAGCA GTTAAAGGAA ACATTTGCAA AGGAACAGCA AGAATTCTTA 
ACAAAATTAG CTTTCGCTGA AGAGCAAAAC AGGAAACTAA TGCTAGAGTT GGAGATAGAG 
CAACAAACTG TGAGATCCGA GATTACAAAC ACCAACAAGC ATTCCATGAG TGCGACTGAT 
GGCTTAAGGC AAGAATGCTT GACTTTAAAC GAAGAGCAAA ATGAGCAGCA AAACGAAGTT 
AGCAACTTAA CACATGAGAA TGAGCAACTG ATGGAGTTAA CACAGACCAA ACATGATTCT 
TATCTCGCAG TAGAGCCAGT TGAGAACTCT GTAAAAGCAA CCGAAGATGA GATAGGTAAG 
AGTAGTTCCC AGTACCAGAT GGATATCGAC ACTAAAGACA TTTCTCTAGA TAGTTATAAG 
GCACAGCTGG TACATCTAGA AGCTTTGGTA AGAATTCTGG AAGTACAGCT TGACCAAAGT 
GAGGAGGAGA ACAAGAAGCT GCATCTGGAA TTACAGACGA TTAGAGAGGA GCTAGAAACC 
AAGAGTTCAC AGGACCCCCA GTCACAGGCA AGGACTGGGC TTAAAGACTG TGACACAGCA 
GAAGAAAAGT ATGTGTCCAT GCTACAGGAG TTGTCAGCAA GTCAAAACGA GAATGCACAC 
TTACAGTGCT CTCTACAGAC AGCAGTGAAC AAACTGAATG AGCTAGGGAA AATGTGTGAC 
GTATTGAGAG TTGAAAAGTT ACAGCTAGAG TCTGAGCTGA ATGACTCACG GACAGAGTGT 
ATCACGGCAA CTAGTCAGAT GACGGCAGAG GTCGAGAAGC TAGTGAGTGA AATGAAAATG 
CTAAACCACG AGAGTGCTCT GTCCCAGAAT GAGCTGATGA AGGACACCTC AGGTGGTGAA 
TTTCATGATA AAGCAAACCA CAGTTCTGTG TTCTTGACTC CTTTGGACAG TAGCAATTTC 
TGTGAACAGA TGACCTTGTC AAGCAAAGAG GTCCGAGTGC ACTTTGCTGA ATTACAGGAG 
AAATTCTCCT GTTTACAAAG TGAACACAAA ATTTTACATG ATCAGCACTG TGAGGTGAGC 
TCTAAGATGT CAGCACTGCG TTCCTACGTG GACACATTAA AAGCTGAAAA TTCTGCCTTG 
TCAATGAGTC TGAGAACCTT GCAGGGTGAC TTGGTAAAGG AGGGGGAGCC TGCAGCTGAG 
GGTGGGCATG GTCTGCCACT GTCGTTCTGT GGGGCAGACA GCCCGTCTCT GACAAATTTT 
GGAGAAACGT CCTTTTACAA AGACGTTTTA GAACAAACTG GAGACACATG TCATCTAAGT 
TTAGAAGGGA ACGCTTCAGC AAATTCTTGT GACTTGGATG AAGAGTTCTC CAGCAGTCTG 
GAGGAGGAGA CTCTGACTGA GAAGGAAAGC CCACCTGCCC CTGGGAGGAC TGTTGAGGGA 
CTTGAAGTCC TATGCCAGGT GTACTTGCAG TCCCTCAAGA ATCTAGAGGA GAAAACTGAG 
AGCCAAAGGA TTATGAAAAA TAAAGAAATT GAAAAGCTTG AGCAGTTACT GAGTTCTGAG 
AGGAAAGAGC TAAGCTGCCT TAGGAAGCAG TATTTGTCAG AAAAGGAGCA ATGGCAGCAG 
AAGCTAACAA GTGTCACTTT GGAAATGGAG TCCAAGTTAG CAGAGGAGAA GCAGCAGACC 
AAGACTCTGT CCCTTGAACT TGAGGTAGCA CGACTTCAGT TACAGGAGCT GGACCTGAGC 
TCCAGGTCTT TGCTTGGCAC TGACTTGGAA AGTGTTGTTC GGTGCCAAAA CGATAATTAT 
GATATAAAAG AATCAGAAGT ATATATTTCA GAGACTACAG AGAAAACACC AAAGCAGGAC 
ACTGACCAAA CTTGTGATAA AGATATTCAG CAGGACCTTG GTCTGGAAAC TTCAGTCACT 
GAGAGTGAGA CTACCAGGCT CACAGGAGAG GGGTGTGAAG AGCAGCCTCC GAAGACCAAT 
TGTGAGGCAC CAGCGGAGGA CAAAACCCAG GACTGCTCAG AATGCATTTC TGAATTGTGT 
TCTAGTTCCA ATGTTTTGGT GCCCATGGAT GTTCTGGAAG ATCAGGGGTC TATCCAGAAT 
CTCCAGTTGC AGAAAGACAC CTTAAATGAG AATTTGAGAT TACTTCCTGA AGTAGAGGAC 
TGGGACAAAA AAGTTGAAAG TTTGCTAAAT GAAATTATGG AGGCAGATTC AAAACTGAGT 
TTACAGGAAG TACAGCTCAA GATGAAGATT GCAACATGCA TACAATTGGA AAAAATAGTC 
AAGGACCTCA GAAAGGAAAA AGCTGACTTA AGTGAAAAGT TGGAATCCCT TCCATGTAAC 
CAGGAGGTAT GTCTGAGAGT AGAAAGGTCA GAAGAAGATC TTGGTTTTAA TTTAGATATG 
GGAGCAAATG AGTTGTTAAG TAAATCTACT AAAGATAATG CAACCAACAC AGAAGACAAT 
TATAAGGAGA AGTTTCTTGA TATGGAAAGA GAACTGACCA GAATTAAGTC TGAGAAAGCT 
AATATTGAGC ATCACATCCT ATCTGTGGAA ACTAACTTAG AGGTGGTTCA AGCAGAGAAG 
CTCTGTTTGG AAAGAGACAC TGAAAGTAAG CAAAAGGTTA TTATTGACCT TAAAGAAGAA 
CTATTTACAG TTATAAGTGA GAGAAACAGA CTTCGGGAAG AATTAGATAA TGTGTCAAAA 
GAAAGCAAAG CACTGGATCA GATGTCTAAA AAGATGAAAG AGAAAATAGA AGAGCTGGAG 
TCTCACCAAA GGGAGAGCCT CCGTCACATT GGGGCAGTAG AGTCTGAGGT CAAGGACAAA 
GCAGATCTTA TTCAGACTCT GTCCTTTAAT GTGGGTGAGC TAACAAAAGA CAAAGCTCAT 
CTCCAGGAGC AGCTGCAGAA TTTGCAGAAT GACTCACAAG AATTATCTTT GGCGATTGGT 
GAGCTGGAAA TACAAATTGG ACAACTGAAT AAAGAGAAAG AATCACTGGT CAAGGAGTCT 
CAGAACTTCC AGATCAAGCT GACTGAGTCA GAGTGTGAAA AGCAGACGAT CTCTAAGGCC 
TTGGAGGTGG CACTCAAGGA GAAAGGTGAG TTTGCAGTGC AGCTGAGCTC AGCCCAGGAG 
GAGGTGCATC AGCTGAGACG AGGCATTGAG AAACTGAGCG TCCGCATTGA GGCCGATGAG 
AAGAAGCACC TCAGTGCTGT GGCGAAGCTG AAAGAAAGCC AGCGTGAAAG CGACTCATTG 
AAGGATACAG TGGAGACTCT GGAGCGGGAA CTGGAGAGGT CAGAAGAAAA CCAAGAGCTG 
GCAATTCTTG ATTCTGAGAA TTTGAAAGCA GAGGTGGAGA CCCTTAAGGC ACAAAAGGAT 
GAAATGACCA AAAGCCTGAG AATTTTCGAA TTAGACCTTG TTACAGTTAG GACTGAAAGA 
GAAAATCTAG CAAAGCAGCT ACAAGAGAAA CAAAGTCGAG TGTCAGAATT AGATGAACGG 
TGCTCTTCCT TGAGAAGACT GTTGGAAGAG AAGGAGCAAG CAAGAGTACA GATGGAAGAA 
GACTCTAAGT CTGCAATGCT GATGCTTCAG ATGCAGTTAA AAGAACTCAG GGAGGAAGTG 
GCAGCCTTGT GTAATGACCA AGAAACCTTG AAGGCCCAAG AACAGAGTCT AGACCAACCA 
GGGGAGGAAG TGCATCATTT GAAAAGTAGC ATTCGAAAGC TCAAAGTTCA CATAGATGCT 
GATGAAAAGA AGCATCAAAA CATCCTAGAA CAACTGAAGG AAAGTAAGCA CCATGCAGAC 
TTGCTTAAGG ACCGAGTTGA GAACCTTGAA CAAGAATTGA TACTATCAGA GAAAAACATG 
ATTTTCCAAG CTGAAAAGTC CAAAGCAGAG ATACAGACTT TAAAATCAGA AATTCAAAGA 
ATGGCCCAAA ACCTCCAAGA CTTGCAGTTA GAACTTATTA GTACAAGGTC AGAAAATGAA 
AATCTCATGA AAGAATTAAA AAAAGAGCAA GAGCGAGTAT CTGACTTAGA AACAATAAAT 
TCTTCTATTG AAAACTTACT GAAAGATAAA GAGCAAGAAA AAGTACAGAT GAAAGAGGAA 
GCCAAAATAA CAGTGGAGAT GCTTCAAACT CAATTAAAGG AGCTAAACGA GACAGTGGTT 
TCCTTGTGCA ATGACCAAGA GGTCTCTAAG ACCAAAGAAC AGAATCTGGG TAGTCAAGTA 
CAAACTCTTG AACTTGAGAA GGCTCAGCTG CTACAGGACC TTGGTGAGGC CAAGAATAAA 
TATATTATTT TTCAGTCATC TGTAAATGCC CTCACTCAAG AAGTAGAAGC TGGCAAACAG 
AAACTAGAGA AGGGGGAGAA AGAGATCAGG ACACTGAAAG AGCAACTTAA AAGTCAGGAG 
CAGCTTGTGT GTAAACTTGC CCAAGTGGAA GGAGAGCAGC AACTCTGGCA GAAGCAGAAA 
CTAGAGCTGA GAAATGTGAC TATGGCACTG GAGCAGAAGG TCCAAGTGCT GCAATCTGAA 
AACAACACGT TGCAGAGCAC CTATGAAGCA CTGCAGAATT CCCACAAGAG TTTAGAGAGT 
GAACTTGGAT TGATAAAGTT GGAGAAAGTA GCGCTTGTTG AAAGAGTTAG CACAATATCT 
GGGAAGGAAG CAGAGCTGCA GAGGGAGCTG CGAGATATGC TACAGAAAAC AACACAGCTG 
AGCGAAGACT ACAATAAAGA GAAAAACAGG CTAACAGAAG AAGTGGAAGT GCTGCGTGAA 
GAACTGCAGA ACACCAAAGC AGCGCACCTG AAATCTGTGA ATCAACTTGA GAAGGAACTT 
CAGCGTGCTC AGGGGAAAAT AAAGTTGATG CTCAAATCCT GTAGACAGCT GGAAGGAGAA 
AAGGAGATGC TGCAGAAGGA GCTCTCCCAG CTTGAAGCTG CACAGCAGCA GAGAGCAGGT 
TCTCTTGTAG ACAGTAACGT AGATGAAGTA ATGACTGAGA ACAAAGCGCT GAAAGAGACT 
CTGGAAGAAA AAGTCAAGGA AGCAGATAAG TACTTGGATA AGTACTGTTC CCTGCTGATA 
AGCCACGAGG AGCTCGAGAA AGCCAAGGAG ATATTAGAAA TAGAAGTTGC TCGGCTGAAG 
TCACGGCAGT CCAGACAGGA TCTCCAGAGT TCTCCTTTGC TTAATTCTTC CATTCCAGGA 
CCGTCTCCAA ATACTTCTGT TAGTGAGATG AAGTCAGCAT CTGGCCAAAA TAAAGCTTCA 
GGCAAGAGGC AAAGGTCCAG TGGGATTTGG GAGCATGGTA AACGGGCAGC ACCTTCTACA 
GCAGAGACAT TTTCTAAGAA AAGCAGGAAG TCGGACAGTA AGAGCACTCG CCCTGCTGAG 
CACGAGCAGG AAACCGAGTT TGAGCCAGAA GGCCTCCCAG AAGTCGTTAA AAAAGGGTTT 
GCTGACATCC CAACTGGAAA GACAAGCCCA TATATCCTTC GGAGAACAAC CATGGCAACC 
AGGACCAGCC CCCGCTTTGC TACACAGAAG TTAGTGGGAT CTTCCCCATC TCTGGGCAAA 
GAAAATGTTG TAGAGTCCTC CAAACCAACA GCTGGTGGCA GCAGATCACA AAAGGTCAAG 
GTTGTTCAGG AGAGCTCAGC GGATTCACAC ACTGCCTTCC AAGAACTCCC AGCAAAATCT 
CTCACAGCCA GTAATATTCC TGGGAGAAAC TCTACAGAGA GCCCCAGGGA GGGCCTGAGG 
GCCAAGCGGG CCTACCCTGC CTCCAGCCCA GCTGCTGGGC CTGATCCCAC GAACAACGAA 
AACTGCCGGG TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497182.1
CDS	595..9372
Translation	MEICEHLEKSRQKLSHELQVKESQVNLQESQLSSCKKQIEKLEQELKRCKSEFERSQQVAQSADVSLNPCSTPQKLFATPLTPSSTYEDLKEKYNKEVEERKRLEEEVKALHAKKVSLPVSQATMNHRDIARHQASSSVFPWQQENTPSRLSSDALKTPLRRDGSAAHFLGEEVSPNKSSMKTGRGDCSSLPGEPHSAQLLHQAKAQNQDLKSKMTELELRLQGQEKEMRSQVNKCQDLQLQLEKTKVELIEKERILNKTRDEVVRSTAQYDQAAAKCTTLEQKLKTLTEELSCHRQNAESAKRSLEQRIKEKEKELQEELSRQHQSFQALDSEYTQMKTRLTQELQQVKHLHSTLQLELEKVTSVKQQLERNLEEIRLKLSRAEQALQASQVAENELRRSSEEMKKENSLIRSQSEQRTREVCHLEEELGKVKVSLSKSQNFAEEMKAKNTSQEIMLRDLQEKLNQQENSLTLEKLKLALADLERQRNCSQDLLKKREHHIDQLNNKLNKIEKEFETLLSALELKKKECEELKEEKNQISFWKIDSEKLINQIESEKEILLGKINHLETSLKTQQVSPDSNERIRTLEMERENFTVEIKNLQSMLDSKMVEIKTQKQAYLELQQKSESSDQKHQKEIENMCLKANKLTGQVESLECKLQLLSSEVVTKDQQYQDLRMEYETLRDLLKSRGSSLVTNEDNQRSSEDNQRSSEDNQRGSLAFEQQPAVSDSFANVMGRKGSINSERSDCSVDGGRSPEHIAILQNRVTSLESSLESQNQMNSDLQMRCEELLQIKGEVEENLSKAEQIHQNFVAETNQCISKLQEDAAVHQNIVAETLATLESKEKELQLLKEKLEAQQTEVQKLNKNNCLLEGTLKELQLLSDTLSSEKKEMNSIISLSKKNIEELTQANEALKEVNEALEQEKMNLLQKHEKITSCIAEQERSIAELSDQYKQERLQLLQRCEETEAVLEDLRGNYKTAQENNAKLECMLSECTALCENRKNELEQLKETFAKEQQEFLTKLAFAEEQNRKLMLELEIEQQTVRSEITNTNKHSMSATDGLRQECLTLNEEQNEQQNEVSNLTHENEQLMELTQTKHDSYLAVEPVENSVKATEDEIGKSSSQYQMDIDTKDISLDSYKAQLVHLEALVRILEVQLDQSEEENKKLHLELQTIREELETKSSQDPQSQARTGLKDCDTAEEKYVSMLQELSASQNENAHLQCSLQTAVNKLNELGKMCDVLRVEKLQLESELNDSRTECITATSQMTAEVEKLVSEMKMLNHESALSQNELMKDTSGGEFHDKANHSSVFLTPLDSSNFCEQMTLSSKEVRVHFAELQEKFSCLQSEHKILHDQHCEVSSKMSALRSYVDTLKAENSALSMSLRTLQGDLVKEGEPAAEGGHGLPLSFCGADSPSLTNFGETSFYKDVLEQTGDTCHLSLEGNASANSCDLDEEFSSSLEEETLTEKESPPAPGRTVEGLEVLCQVYLQSLKNLEEKTESQRIMKNKEIEKLEQLLSSERKELSCLRKQYLSEKEQWQQKLTSVTLEMESKLAEEKQQTKTLSLELEVARLQLQELDLSSRSLLGTDLESVVRCQNDNYDIKESEVYISETTEKTPKQDTDQTCDKDIQQDLGLETSVTESETTRLTGEGCEEQPPKTNCEAPAEDKTQDCSECISELCSSSNVLVPMDVLEDQGSIQNLQLQKDTLNENLRLLPEVEDWDKKVESLLNEIMEADSKLSLQEVQLKMKIATCIQLEKIVKDLRKEKADLSEKLESLPCNQEVCLRVERSEEDLGFNLDMGANELLSKSTKDNATNTEDNYKEKFLDMERELTRIKSEKANIEHHILSVETNLEVVQAEKLCLERDTESKQKVIIDLKEELFTVISERNRLREELDNVSKESKALDQMSKKMKEKIEELESHQRESLRHIGAVESEVKDKADLIQTLSFNVGELTKDKAHLQEQLQNLQNDSQELSLAIGELEIQIGQLNKEKESLVKESQNFQIKLTESECEKQTISKALEVALKEKGEFAVQLSSAQEEVHQLRRGIEKLSVRIEADEKKHLSAVAKLKESQRESDSLKDTVETLERELERSEENQELAILDSENLKAEVETLKAQKDEMTKSLRIFELDLVTVRTERENLAKQLQEKQSRVSELDERCSSLRRLLEEKEQARVQMEEDSKSAMLMLQMQLKELREEVAALCNDQETLKAQEQSLDQPGEEVHHLKSSIRKLKVHIDADEKKHQNILEQLKESKHHADLLKDRVENLEQELILSEKNMIFQAEKSKAEIQTLKSEIQRMAQNLQDLQLELISTRSENENLMKELKKEQERVSDLETINSSIENLLKDKEQEKVQMKEEAKITVEMLQTQLKELNETVVSLCNDQEVSKTKEQNLGSQVQTLELEKAQLLQDLGEAKNKYIIFQSSVNALTQEVEAGKQKLEKGEKEIRTLKEQLKSQEQLVCKLAQVEGEQQLWQKQKLELRNVTMALEQKVQVLQSENNTLQSTYEALQNSHKSLESELGLIKLEKVALVERVSTISGKEAELQRELRDMLQKTTQLSEDYNKEKNRLTEEVEVLREELQNTKAAHLKSVNQLEKELQRAQGKIKLMLKSCRQLEGEKEMLQKELSQLEAAQQQRAGSLVDSNVDEVMTENKALKETLEEKVKEADKYLDKYCSLLISHEELEKAKEILEIEVARLKSRQSRQDLQSSPLLNSSIPGPSPNTSVSEMKSASGQNKASGKRQRSSGIWEHGKRAAPSTAETFSKKSRKSDSKSTRPAEHEQETEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRFATQKLVGSSPSLGKENVVESSKPTAGGSRSQKVKVVQESSADSHTAFQELPAKSLTASNIPGRNSTESPREGLRAKRAYPASSPAAGPDPTNNENCRVQ

Target ORF information:

RefSeq Version	XM_006497119.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus centromere protein F (Cenpf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497119.2

ORF Insert Sequence:

ATGGAGATAT GCGAACATTT GGAGAAGTCA AGGCAGAAGC TGTCTCATGA ACTTCAAGTT 
AAGGAGTCAC AAGTGAATCT CCAAGAGAGC CAACTGAGCT CATGCAAAAA GCAAATAGAA 
AAACTGGAAC AGGAACTTAA GCGGTGTAAA TCTGAATTTG AAAGAAGCCA ACAAGTTGCA 
CAATCGGCAG ATGTTTCTCT GAATCCATGC AGTACACCAC AGAAACTCTT TGCAACTCCA 
CTCACACCGA GTTCCACATA CGAAGATCTG AAAGAAAAAT ATAATAAAGA AGTTGAAGAA 
CGGAAGAGGT TAGAGGAAGA GGTTAAAGCT TTGCATGCAA AAAAAGTGAG CCTGCCTGTT 
TCCCAAGCCA CCATGAACCA CCGGGACATT GCGAGACATC AGGCTTCCTC ATCAGTGTTT 
CCTTGGCAAC AGGAAAATAC CCCAAGTCGC CTTTCATCGG ATGCTCTGAA AACCCCACTG 
AGGAGAGACG GCTCTGCTGC TCACTTTTTG GGGGAAGAAG TGAGTCCTAA CAAATCAAGT 
ATGAAGACAG GGAGAGGAGA CTGCAGCAGC CTCCCTGGTG AGCCTCACAG CGCTCAGCTT 
TTGCACCAGG CCAAAGCCCA GAATCAAGAC CTAAAAAGCA AGATGACTGA GTTAGAACTA 
CGCCTGCAAG GGCAAGAAAA GGAAATGAGA AGCCAAGTGA ATAAATGTCA AGACTTACAG 
CTACAGCTGG AGAAGACGAA AGTGGAATTG ATTGAAAAGG AGAGAATTTT GAATAAAACC 
AGAGATGAAG TAGTGAGAAG CACAGCACAG TATGACCAGG CCGCAGCCAA GTGTACTACC 
TTGGAACAAA AGCTGAAAAC TTTGACTGAG GAGTTGAGTT GTCACCGACA GAACGCAGAG 
AGTGCTAAAC GTTCTCTGGA ACAGAGGATT AAGGAGAAAG AAAAGGAGCT TCAAGAGGAG 
CTGTCCCGAC AGCATCAATC TTTCCAAGCT CTGGACAGTG AGTACACTCA GATGAAAACC 
AGACTTACCC AGGAGTTACA GCAAGTCAAG CATTTGCACA GCACCCTCCA GCTGGAACTG 
GAGAAGGTCA CATCAGTGAA GCAGCAGTTA GAAAGGAATT TGGAAGAAAT TAGGCTTAAG 
TTGAGCAGAG CAGAACAAGC TCTTCAGGCA AGTCAGGTCG CAGAAAACGA GCTGAGGAGA 
AGCAGTGAGG AAATGAAGAA GGAGAACAGT CTCATTAGGA GTCAGTCTGA GCAGAGGACC 
AGAGAAGTCT GCCACCTGGA GGAAGAACTT GGTAAAGTCA AAGTGTCTTT GAGTAAGAGC 
CAGAATTTTG CAGAAGAAAT GAAGGCTAAG AATACCTCTC AGGAAATCAT GTTACGAGAT 
CTTCAGGAAA AACTAAATCA GCAAGAAAAC TCACTAACTT TAGAGAAGCT GAAACTTGCC 
CTAGCTGATC TGGAAAGACA GCGAAACTGT TCTCAAGATC TCTTGAAGAA AAGGGAACAT 
CACATTGATC AACTGAATAA TAAGTTAAAT AAGATAGAGA AAGAGTTTGA AACTTTGCTG 
AGTGCTTTGG AATTAAAAAA GAAAGAATGT GAAGAATTGA AAGAAGAGAA AAATCAGATT 
TCTTTTTGGA AAATTGATAG TGAAAAACTC ATAAATCAGA TAGAATCAGA AAAAGAAATC 
TTATTGGGTA AAATTAACCA CTTAGAGACC AGCCTCAAGA CACAACAAGT AAGTCCTGAC 
TCTAATGAGA GAATAAGAAC ACTGGAGATG GAAAGAGAAA ACTTTACTGT GGAGATTAAA 
AACCTTCAAA GTATGTTAGA CAGCAAGATG GTAGAGATCA AGACACAGAA ACAAGCTTAC 
TTGGAACTGC AGCAGAAATC CGAATCCTCG GACCAAAAGC ATCAGAAGGA GATAGAGAAT 
ATGTGCTTGA AAGCAAATAA GCTCACTGGG CAAGTTGAAA GTTTGGAATG TAAGCTTCAG 
TTATTGTCAA GTGAAGTAGT GACCAAAGAC CAGCAGTACC AAGACTTGCG TATGGAATAT 
GAGACACTGA GGGATTTGCT CAAGTCCAGA GGATCTTCTC TGGTGACAAA TGAGGATAAT 
CAGCGAAGTT CTGAGGATAA TCAGAGAAGT TCTGAGGATA ATCAGAGAGG CTCTTTGGCT 
TTTGAACAGC AGCCTGCAGT GAGTGATTCC TTTGCAAATG TAATGGGGAG AAAAGGAAGC 
ATAAATTCAG AAAGGAGTGA CTGCTCTGTA GATGGGGGCC GAAGTCCAGA ACATATAGCC 
ATCTTACAAA ATAGAGTCAC TTCACTTGAA AGTTCCTTGG AGTCTCAAAA CCAGATGAAT 
TCAGATTTGC AAATGCGGTG TGAAGAGTTG CTACAAATCA AAGGGGAAGT AGAAGAAAAC 
CTCAGTAAAG CAGAGCAGAT TCATCAGAAT TTTGTGGCTG AAACAAATCA ATGTATTAGT 
AAATTGCAGG AAGATGCTGC AGTTCATCAG AATATTGTTG CTGAGACTTT AGCAACCCTT 
GAGAGTAAGG AAAAAGAGTT ACAGCTTTTG AAAGAAAAAT TAGAAGCTCA GCAAACAGAG 
GTTCAAAAGT TAAATAAGAA TAACTGTCTT CTTGAAGGTA CTCTGAAGGA GCTACAGCTT 
TTATCTGACA CTCTGAGCTC AGAGAAGAAG GAAATGAATT CTATCATCTC ATTAAGTAAA 
AAAAACATTG AAGAGTTAAC CCAAGCAAAC GAGGCTCTCA AGGAAGTTAA TGAGGCCTTA 
GAGCAGGAGA AAATGAATTT ACTCCAAAAG CATGAGAAGA TTACAAGCTG TATAGCAGAA 
CAAGAGAGAA GCATTGCAGA GCTGTCTGAT CAGTACAAGC AAGAAAGACT TCAATTATTA 
CAACGGTGTG AAGAAACAGA AGCTGTGTTG GAAGATCTCA GGGGAAACTA CAAAACAGCA 
CAAGAAAACA ATGCTAAGTT AGAATGCATG CTCAGCGAGT GCACTGCTCT TTGTGAAAAT 
AGAAAAAATG AACTGGAGCA GTTAAAGGAA ACATTTGCAA AGGAACAGCA AGAATTCTTA 
ACAAAATTAG CTTTCGCTGA AGAGCAAAAC AGGAAACTAA TGCTAGAGTT GGAGATAGAG 
CAACAAACTG TGAGATCCGA GATTACAAAC ACCAACAAGC ATTCCATGAG TGCGACTGAT 
GGCTTAAGGC AAGAATGCTT GACTTTAAAC GAAGAGCAAA ATGAGCAGCA AAACGAAGTT 
AGCAACTTAA CACATGAGAA TGAGCAACTG ATGGAGTTAA CACAGACCAA ACATGATTCT 
TATCTCGCAG TAGAGCCAGT TGAGAACTCT GTAAAAGCAA CCGAAGATGA GATAGGTAAG 
AGTAGTTCCC AGTACCAGAT GGATATCGAC ACTAAAGACA TTTCTCTAGA TAGTTATAAG 
GCACAGCTGG TACATCTAGA AGCTTTGGTA AGAATTCTGG AAGTACAGCT TGACCAAAGT 
GAGGAGGAGA ACAAGAAGCT GCATCTGGAA TTACAGACGA TTAGAGAGGA GCTAGAAACC 
AAGAGTTCAC AGGACCCCCA GTCACAGGCA AGGACTGGGC TTAAAGACTG TGACACAGCA 
GAAGAAAAGT ATGTGTCCAT GCTACAGGAG TTGTCAGCAA GTCAAAACGA GAATGCACAC 
TTACAGTGCT CTCTACAGAC AGCAGTGAAC AAACTGAATG AGCTAGGGAA AATGTGTGAC 
GTATTGAGAG TTGAAAAGTT ACAGCTAGAG TCTGAGCTGA ATGACTCACG GACAGAGTGT 
ATCACGGCAA CTAGTCAGAT GACGGCAGAG GTCGAGAAGC TAGTGAGTGA AATGAAAATG 
CTAAACCACG AGAGTGCTCT GTCCCAGAAT GAGCTGATGA AGGACACCTC AGGTGGTGAA 
TTTCATGATA AAGCAAACCA CAGTTCTGTG TTCTTGACTC CTTTGGACAG TAGCAATTTC 
TGTGAACAGA TGACCTTGTC AAGCAAAGAG GTCCGAGTGC ACTTTGCTGA ATTACAGGAG 
AAATTCTCCT GTTTACAAAG TGAACACAAA ATTTTACATG ATCAGCACTG TGAGGTGAGC 
TCTAAGATGT CAGCACTGCG TTCCTACGTG GACACATTAA AAGCTGAAAA TTCTGCCTTG 
TCAATGAGTC TGAGAACCTT GCAGGGTGAC TTGGTAAAGG AGGGGGAGCC TGCAGCTGAG 
GGTGGGCATG GTCTGCCACT GTCGTTCTGT GGGGCAGACA GCCCGTCTCT GACAAATTTT 
GGAGAAACGT CCTTTTACAA AGACGTTTTA GAACAAACTG GAGACACATG TCATCTAAGT 
TTAGAAGGGA ACGCTTCAGC AAATTCTTGT GACTTGGATG AAGAGTTCTC CAGCAGTCTG 
GAGGAGGAGA CTCTGACTGA GAAGGAAAGC CCACCTGCCC CTGGGAGGAC TGTTGAGGGA 
CTTGAAGTCC TATGCCAGGT GTACTTGCAG TCCCTCAAGA ATCTAGAGGA GAAAACTGAG 
AGCCAAAGGA TTATGAAAAA TAAAGAAATT GAAAAGCTTG AGCAGTTACT GAGTTCTGAG 
AGGAAAGAGC TAAGCTGCCT TAGGAAGCAG TATTTGTCAG AAAAGGAGCA ATGGCAGCAG 
AAGCTAACAA GTGTCACTTT GGAAATGGAG TCCAAGTTAG CAGAGGAGAA GCAGCAGACC 
AAGACTCTGT CCCTTGAACT TGAGGTAGCA CGACTTCAGT TACAGGAGCT GGACCTGAGC 
TCCAGGTCTT TGCTTGGCAC TGACTTGGAA AGTGTTGTTC GGTGCCAAAA CGATAATTAT 
GATATAAAAG AATCAGAAGT ATATATTTCA GAGACTACAG AGAAAACACC AAAGCAGGAC 
ACTGACCAAA CTTGTGATAA AGATATTCAG CAGGACCTTG GTCTGGAAAC TTCAGTCACT 
GAGAGTGAGA CTACCAGGCT CACAGGAGAG GGGTGTGAAG AGCAGCCTCC GAAGACCAAT 
TGTGAGGCAC CAGCGGAGGA CAAAACCCAG GACTGCTCAG AATGCATTTC TGAATTGTGT 
TCTAGTTCCA ATGTTTTGGT GCCCATGGAT GTTCTGGAAG ATCAGGGGTC TATCCAGAAT 
CTCCAGTTGC AGAAAGACAC CTTAAATGAG AATTTGAGAT TACTTCCTGA AGTAGAGGAC 
TGGGACAAAA AAGTTGAAAG TTTGCTAAAT GAAATTATGG AGGCAGATTC AAAACTGAGT 
TTACAGGAAG TACAGCTCAA GATGAAGATT GCAACATGCA TACAATTGGA AAAAATAGTC 
AAGGACCTCA GAAAGGAAAA AGCTGACTTA AGTGAAAAGT TGGAATCCCT TCCATGTAAC 
CAGGAGGTAT GTCTGAGAGT AGAAAGGTCA GAAGAAGATC TTGGTTTTAA TTTAGATATG 
GGAGCAAATG AGTTGTTAAG TAAATCTACT AAAGATAATG CAACCAACAC AGAAGACAAT 
TATAAGGAGA AGTTTCTTGA TATGGAAAGA GAACTGACCA GAATTAAGTC TGAGAAAGCT 
AATATTGAGC ATCACATCCT ATCTGTGGAA ACTAACTTAG AGGTGGTTCA AGCAGAGAAG 
CTCTGTTTGG AAAGAGACAC TGAAAGTAAG CAAAAGGTTA TTATTGACCT TAAAGAAGAA 
CTATTTACAG TTATAAGTGA GAGAAACAGA CTTCGGGAAG AATTAGATAA TGTGTCAAAA 
GAAAGCAAAG CACTGGATCA GATGTCTAAA AAGATGAAAG AGAAAATAGA AGAGCTGGAG 
TCTCACCAAA GGGAGAGCCT CCGTCACATT GGGGCAGTAG AGTCTGAGGT CAAGGACAAA 
GCAGATCTTA TTCAGACTCT GTCCTTTAAT GTGGGTGAGC TAACAAAAGA CAAAGCTCAT 
CTCCAGGAGC AGCTGCAGAA TTTGCAGAAT GACTCACAAG AATTATCTTT GGCGATTGGT 
GAGCTGGAAA TACAAATTGG ACAACTGAAT AAAGAGAAAG AATCACTGGT CAAGGAGTCT 
CAGAACTTCC AGATCAAGCT GACTGAGTCA GAGTGTGAAA AGCAGACGAT CTCTAAGGCC 
TTGGAGGTGG CACTCAAGGA GAAAGGTGAG TTTGCAGTGC AGCTGAGCTC AGCCCAGGAG 
GAGGTGCATC AGCTGAGACG AGGCATTGAG AAACTGAGCG TCCGCATTGA GGCCGATGAG 
AAGAAGCACC TCAGTGCTGT GGCGAAGCTG AAAGAAAGCC AGCGTGAAAG CGACTCATTG 
AAGGATACAG TGGAGACTCT GGAGCGGGAA CTGGAGAGGT CAGAAGAAAA CCAAGAGCTG 
GCAATTCTTG ATTCTGAGAA TTTGAAAGCA GAGGTGGAGA CCCTTAAGGC ACAAAAGGAT 
GAAATGACCA AAAGCCTGAG AATTTTCGAA TTAGACCTTG TTACAGTTAG GACTGAAAGA 
GAAAATCTAG CAAAGCAGCT ACAAGAGAAA CAAAGTCGAG TGTCAGAATT AGATGAACGG 
TGCTCTTCCT TGAGAAGACT GTTGGAAGAG AAGGAGCAAG CAAGAGTACA GATGGAAGAA 
GACTCTAAGT CTGCAATGCT GATGCTTCAG ATGCAGTTAA AAGAACTCAG GGAGGAAGTG 
GCAGCCTTGT GTAATGACCA AGAAACCTTG AAGGCCCAAG AACAGAGTCT AGACCAACCA 
GGGGAGGAAG TGCATCATTT GAAAAGTAGC ATTCGAAAGC TCAAAGTTCA CATAGATGCT 
GATGAAAAGA AGCATCAAAA CATCCTAGAA CAACTGAAGG AAAGTAAGCA CCATGCAGAC 
TTGCTTAAGG ACCGAGTTGA GAACCTTGAA CAAGAATTGA TACTATCAGA GAAAAACATG 
ATTTTCCAAG CTGAAAAGTC CAAAGCAGAG ATACAGACTT TAAAATCAGA AATTCAAAGA 
ATGGCCCAAA ACCTCCAAGA CTTGCAGTTA GAACTTATTA GTACAAGGTC AGAAAATGAA 
AATCTCATGA AAGAATTAAA AAAAGAGCAA GAGCGAGTAT CTGACTTAGA AACAATAAAT 
TCTTCTATTG AAAACTTACT GAAAGATAAA GAGCAAGAAA AAGTACAGAT GAAAGAGGAA 
GCCAAAATAA CAGTGGAGAT GCTTCAAACT CAATTAAAGG AGCTAAACGA GACAGTGGTT 
TCCTTGTGCA ATGACCAAGA GGTCTCTAAG ACCAAAGAAC AGAATCTGGG TAGTCAAGTA 
CAAACTCTTG AACTTGAGAA GGCTCAGCTG CTACAGGACC TTGGTGAGGC CAAGAATAAA 
TATATTATTT TTCAGTCATC TGTAAATGCC CTCACTCAAG AAGTAGAAGC TGGCAAACAG 
AAACTAGAGA AGGGGGAGAA AGAGATCAGG ACACTGAAAG AGCAACTTAA AAGTCAGGAG 
CAGCTTGTGT GTAAACTTGC CCAAGTGGAA GGAGAGCAGC AACTCTGGCA GAAGCAGAAA 
CTAGAGCTGA GAAATGTGAC TATGGCACTG GAGCAGAAGG TCCAAGTGCT GCAATCTGAA 
AACAACACGT TGCAGAGCAC CTATGAAGCA CTGCAGAATT CCCACAAGAG TTTAGAGAGT 
GAACTTGGAT TGATAAAGTT GGAGAAAGTA GCGCTTGTTG AAAGAGTTAG CACAATATCT 
GGGAAGGAAG CAGAGCTGCA GAGGGAGCTG CGAGATATGC TACAGAAAAC AACACAGCTG 
AGCGAAGACT ACAATAAAGA GAAAAACAGG CTAACAGAAG AAGTGGAAGT GCTGCGTGAA 
GAACTGCAGA ACACCAAAGC AGCGCACCTG AAATCTGTGA ATCAACTTGA GAAGGAACTT 
CAGCGTGCTC AGGGGAAAAT AAAGTTGATG CTCAAATCCT GTAGACAGCT GGAAGGAGAA 
AAGGAGATGC TGCAGAAGGA GCTCTCCCAG CTTGAAGCTG CACAGCAGCA GAGAGCAGGT 
TCTCTTGTAG ACAGTAACGT AGATGAAGTA ATGACTGAGA ACAAAGCGCT GAAAGAGACT 
CTGGAAGAAA AAGTCAAGGA AGCAGATAAG TACTTGGATA AGTACTGTTC CCTGCTGATA 
AGCCACGAGG AGCTCGAGAA AGCCAAGGAG ATATTAGAAA TAGAAGTTGC TCGGCTGAAG 
TCACGGCAGT CCAGACAGGA TCTCCAGAGT TCTCCTTTGC TTAATTCTTC CATTCCAGGA 
CCGTCTCCAA ATACTTCTGT TAGTGAGATG AAGTCAGCAT CTGGCCAAAA TAAAGCTTCA 
GGCAAGAGGC AAAGGTCCAG TGGGATTTGG GAGCATGGTA AACGGGCAGC ACCTTCTACA 
GCAGAGACAT TTTCTAAGAA AAGCAGGAAG TCGGACAGTA AGAGCACTCG CCCTGCTGAG 
CACGAGCAGG AAACCGAGTT TGAGCCAGAA GGCCTCCCAG AAGTCGTTAA AAAAGGGTTT 
GCTGACATCC CAACTGGAAA GACAAGCCCA TATATCCTTC GGAGAACAAC CATGGCAACC 
AGGACCAGCC CCCGCTTTGC TACACAGAAG TTAGTGGGAT CTTCCCCATC TCTGGGCAAA 
GAAAATGTTG TAGAGTCCTC CAAACCAACA GCTGGTGGCA GCAGATCACA AAAGGTCAAG 
GTTGTTCAGG AGAGCTCAGC GGATTCACAC ACTGCCTTCC AAGAACTCCC AGCAAAATCT 
CTCACAGCCA GTAATATTCC TGGGAGAAAC TCTACAGAGA GCCCCAGGGA GGGCCTGAGG 
GCCAAGCGGG CCTACCCTGC CTCCAGCCCA GCTGCTGGGC CTGATCCCAC GAACAACGAA 
AACTGCCGGG TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cenpf ( XM_006497119.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497182.1 Mus musculus centromere protein F (Cenpf), transcript variant X1, mRNA.

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CloneID	OMu28933
Clone ID Related Accession (Same CDS sequence)	XM_017320925.1 , XM_006497119.2 , XM_006497119.3
Accession Version	XM_006497119.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	centromere protein F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497119.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATAT GCGAACATTT GGAGAAGTCA AGGCAGAAGC TGTCTCATGA ACTTCAAGTT 
AAGGAGTCAC AAGTGAATCT CCAAGAGAGC CAACTGAGCT CATGCAAAAA GCAAATAGAA 
AAACTGGAAC AGGAACTTAA GCGGTGTAAA TCTGAATTTG AAAGAAGCCA ACAAGTTGCA 
CAATCGGCAG ATGTTTCTCT GAATCCATGC AGTACACCAC AGAAACTCTT TGCAACTCCA 
CTCACACCGA GTTCCACATA CGAAGATCTG AAAGAAAAAT ATAATAAAGA AGTTGAAGAA 
CGGAAGAGGT TAGAGGAAGA GGTTAAAGCT TTGCATGCAA AAAAAGTGAG CCTGCCTGTT 
TCCCAAGCCA CCATGAACCA CCGGGACATT GCGAGACATC AGGCTTCCTC ATCAGTGTTT 
CCTTGGCAAC AGGAAAATAC CCCAAGTCGC CTTTCATCGG ATGCTCTGAA AACCCCACTG 
AGGAGAGACG GCTCTGCTGC TCACTTTTTG GGGGAAGAAG TGAGTCCTAA CAAATCAAGT 
ATGAAGACAG GGAGAGGAGA CTGCAGCAGC CTCCCTGGTG AGCCTCACAG CGCTCAGCTT 
TTGCACCAGG CCAAAGCCCA GAATCAAGAC CTAAAAAGCA AGATGACTGA GTTAGAACTA 
CGCCTGCAAG GGCAAGAAAA GGAAATGAGA AGCCAAGTGA ATAAATGTCA AGACTTACAG 
CTACAGCTGG AGAAGACGAA AGTGGAATTG ATTGAAAAGG AGAGAATTTT GAATAAAACC 
AGAGATGAAG TAGTGAGAAG CACAGCACAG TATGACCAGG CCGCAGCCAA GTGTACTACC 
TTGGAACAAA AGCTGAAAAC TTTGACTGAG GAGTTGAGTT GTCACCGACA GAACGCAGAG 
AGTGCTAAAC GTTCTCTGGA ACAGAGGATT AAGGAGAAAG AAAAGGAGCT TCAAGAGGAG 
CTGTCCCGAC AGCATCAATC TTTCCAAGCT CTGGACAGTG AGTACACTCA GATGAAAACC 
AGACTTACCC AGGAGTTACA GCAAGTCAAG CATTTGCACA GCACCCTCCA GCTGGAACTG 
GAGAAGGTCA CATCAGTGAA GCAGCAGTTA GAAAGGAATT TGGAAGAAAT TAGGCTTAAG 
TTGAGCAGAG CAGAACAAGC TCTTCAGGCA AGTCAGGTCG CAGAAAACGA GCTGAGGAGA 
AGCAGTGAGG AAATGAAGAA GGAGAACAGT CTCATTAGGA GTCAGTCTGA GCAGAGGACC 
AGAGAAGTCT GCCACCTGGA GGAAGAACTT GGTAAAGTCA AAGTGTCTTT GAGTAAGAGC 
CAGAATTTTG CAGAAGAAAT GAAGGCTAAG AATACCTCTC AGGAAATCAT GTTACGAGAT 
CTTCAGGAAA AACTAAATCA GCAAGAAAAC TCACTAACTT TAGAGAAGCT GAAACTTGCC 
CTAGCTGATC TGGAAAGACA GCGAAACTGT TCTCAAGATC TCTTGAAGAA AAGGGAACAT 
CACATTGATC AACTGAATAA TAAGTTAAAT AAGATAGAGA AAGAGTTTGA AACTTTGCTG 
AGTGCTTTGG AATTAAAAAA GAAAGAATGT GAAGAATTGA AAGAAGAGAA AAATCAGATT 
TCTTTTTGGA AAATTGATAG TGAAAAACTC ATAAATCAGA TAGAATCAGA AAAAGAAATC 
TTATTGGGTA AAATTAACCA CTTAGAGACC AGCCTCAAGA CACAACAAGT AAGTCCTGAC 
TCTAATGAGA GAATAAGAAC ACTGGAGATG GAAAGAGAAA ACTTTACTGT GGAGATTAAA 
AACCTTCAAA GTATGTTAGA CAGCAAGATG GTAGAGATCA AGACACAGAA ACAAGCTTAC 
TTGGAACTGC AGCAGAAATC CGAATCCTCG GACCAAAAGC ATCAGAAGGA GATAGAGAAT 
ATGTGCTTGA AAGCAAATAA GCTCACTGGG CAAGTTGAAA GTTTGGAATG TAAGCTTCAG 
TTATTGTCAA GTGAAGTAGT GACCAAAGAC CAGCAGTACC AAGACTTGCG TATGGAATAT 
GAGACACTGA GGGATTTGCT CAAGTCCAGA GGATCTTCTC TGGTGACAAA TGAGGATAAT 
CAGCGAAGTT CTGAGGATAA TCAGAGAAGT TCTGAGGATA ATCAGAGAGG CTCTTTGGCT 
TTTGAACAGC AGCCTGCAGT GAGTGATTCC TTTGCAAATG TAATGGGGAG AAAAGGAAGC 
ATAAATTCAG AAAGGAGTGA CTGCTCTGTA GATGGGGGCC GAAGTCCAGA ACATATAGCC 
ATCTTACAAA ATAGAGTCAC TTCACTTGAA AGTTCCTTGG AGTCTCAAAA CCAGATGAAT 
TCAGATTTGC AAATGCGGTG TGAAGAGTTG CTACAAATCA AAGGGGAAGT AGAAGAAAAC 
CTCAGTAAAG CAGAGCAGAT TCATCAGAAT TTTGTGGCTG AAACAAATCA ATGTATTAGT 
AAATTGCAGG AAGATGCTGC AGTTCATCAG AATATTGTTG CTGAGACTTT AGCAACCCTT 
GAGAGTAAGG AAAAAGAGTT ACAGCTTTTG AAAGAAAAAT TAGAAGCTCA GCAAACAGAG 
GTTCAAAAGT TAAATAAGAA TAACTGTCTT CTTGAAGGTA CTCTGAAGGA GCTACAGCTT 
TTATCTGACA CTCTGAGCTC AGAGAAGAAG GAAATGAATT CTATCATCTC ATTAAGTAAA 
AAAAACATTG AAGAGTTAAC CCAAGCAAAC GAGGCTCTCA AGGAAGTTAA TGAGGCCTTA 
GAGCAGGAGA AAATGAATTT ACTCCAAAAG CATGAGAAGA TTACAAGCTG TATAGCAGAA 
CAAGAGAGAA GCATTGCAGA GCTGTCTGAT CAGTACAAGC AAGAAAGACT TCAATTATTA 
CAACGGTGTG AAGAAACAGA AGCTGTGTTG GAAGATCTCA GGGGAAACTA CAAAACAGCA 
CAAGAAAACA ATGCTAAGTT AGAATGCATG CTCAGCGAGT GCACTGCTCT TTGTGAAAAT 
AGAAAAAATG AACTGGAGCA GTTAAAGGAA ACATTTGCAA AGGAACAGCA AGAATTCTTA 
ACAAAATTAG CTTTCGCTGA AGAGCAAAAC AGGAAACTAA TGCTAGAGTT GGAGATAGAG 
CAACAAACTG TGAGATCCGA GATTACAAAC ACCAACAAGC ATTCCATGAG TGCGACTGAT 
GGCTTAAGGC AAGAATGCTT GACTTTAAAC GAAGAGCAAA ATGAGCAGCA AAACGAAGTT 
AGCAACTTAA CACATGAGAA TGAGCAACTG ATGGAGTTAA CACAGACCAA ACATGATTCT 
TATCTCGCAG TAGAGCCAGT TGAGAACTCT GTAAAAGCAA CCGAAGATGA GATAGGTAAG 
AGTAGTTCCC AGTACCAGAT GGATATCGAC ACTAAAGACA TTTCTCTAGA TAGTTATAAG 
GCACAGCTGG TACATCTAGA AGCTTTGGTA AGAATTCTGG AAGTACAGCT TGACCAAAGT 
GAGGAGGAGA ACAAGAAGCT GCATCTGGAA TTACAGACGA TTAGAGAGGA GCTAGAAACC 
AAGAGTTCAC AGGACCCCCA GTCACAGGCA AGGACTGGGC TTAAAGACTG TGACACAGCA 
GAAGAAAAGT ATGTGTCCAT GCTACAGGAG TTGTCAGCAA GTCAAAACGA GAATGCACAC 
TTACAGTGCT CTCTACAGAC AGCAGTGAAC AAACTGAATG AGCTAGGGAA AATGTGTGAC 
GTATTGAGAG TTGAAAAGTT ACAGCTAGAG TCTGAGCTGA ATGACTCACG GACAGAGTGT 
ATCACGGCAA CTAGTCAGAT GACGGCAGAG GTCGAGAAGC TAGTGAGTGA AATGAAAATG 
CTAAACCACG AGAGTGCTCT GTCCCAGAAT GAGCTGATGA AGGACACCTC AGGTGGTGAA 
TTTCATGATA AAGCAAACCA CAGTTCTGTG TTCTTGACTC CTTTGGACAG TAGCAATTTC 
TGTGAACAGA TGACCTTGTC AAGCAAAGAG GTCCGAGTGC ACTTTGCTGA ATTACAGGAG 
AAATTCTCCT GTTTACAAAG TGAACACAAA ATTTTACATG ATCAGCACTG TGAGGTGAGC 
TCTAAGATGT CAGCACTGCG TTCCTACGTG GACACATTAA AAGCTGAAAA TTCTGCCTTG 
TCAATGAGTC TGAGAACCTT GCAGGGTGAC TTGGTAAAGG AGGGGGAGCC TGCAGCTGAG 
GGTGGGCATG GTCTGCCACT GTCGTTCTGT GGGGCAGACA GCCCGTCTCT GACAAATTTT 
GGAGAAACGT CCTTTTACAA AGACGTTTTA GAACAAACTG GAGACACATG TCATCTAAGT 
TTAGAAGGGA ACGCTTCAGC AAATTCTTGT GACTTGGATG AAGAGTTCTC CAGCAGTCTG 
GAGGAGGAGA CTCTGACTGA GAAGGAAAGC CCACCTGCCC CTGGGAGGAC TGTTGAGGGA 
CTTGAAGTCC TATGCCAGGT GTACTTGCAG TCCCTCAAGA ATCTAGAGGA GAAAACTGAG 
AGCCAAAGGA TTATGAAAAA TAAAGAAATT GAAAAGCTTG AGCAGTTACT GAGTTCTGAG 
AGGAAAGAGC TAAGCTGCCT TAGGAAGCAG TATTTGTCAG AAAAGGAGCA ATGGCAGCAG 
AAGCTAACAA GTGTCACTTT GGAAATGGAG TCCAAGTTAG CAGAGGAGAA GCAGCAGACC 
AAGACTCTGT CCCTTGAACT TGAGGTAGCA CGACTTCAGT TACAGGAGCT GGACCTGAGC 
TCCAGGTCTT TGCTTGGCAC TGACTTGGAA AGTGTTGTTC GGTGCCAAAA CGATAATTAT 
GATATAAAAG AATCAGAAGT ATATATTTCA GAGACTACAG AGAAAACACC AAAGCAGGAC 
ACTGACCAAA CTTGTGATAA AGATATTCAG CAGGACCTTG GTCTGGAAAC TTCAGTCACT 
GAGAGTGAGA CTACCAGGCT CACAGGAGAG GGGTGTGAAG AGCAGCCTCC GAAGACCAAT 
TGTGAGGCAC CAGCGGAGGA CAAAACCCAG GACTGCTCAG AATGCATTTC TGAATTGTGT 
TCTAGTTCCA ATGTTTTGGT GCCCATGGAT GTTCTGGAAG ATCAGGGGTC TATCCAGAAT 
CTCCAGTTGC AGAAAGACAC CTTAAATGAG AATTTGAGAT TACTTCCTGA AGTAGAGGAC 
TGGGACAAAA AAGTTGAAAG TTTGCTAAAT GAAATTATGG AGGCAGATTC AAAACTGAGT 
TTACAGGAAG TACAGCTCAA GATGAAGATT GCAACATGCA TACAATTGGA AAAAATAGTC 
AAGGACCTCA GAAAGGAAAA AGCTGACTTA AGTGAAAAGT TGGAATCCCT TCCATGTAAC 
CAGGAGGTAT GTCTGAGAGT AGAAAGGTCA GAAGAAGATC TTGGTTTTAA TTTAGATATG 
GGAGCAAATG AGTTGTTAAG TAAATCTACT AAAGATAATG CAACCAACAC AGAAGACAAT 
TATAAGGAGA AGTTTCTTGA TATGGAAAGA GAACTGACCA GAATTAAGTC TGAGAAAGCT 
AATATTGAGC ATCACATCCT ATCTGTGGAA ACTAACTTAG AGGTGGTTCA AGCAGAGAAG 
CTCTGTTTGG AAAGAGACAC TGAAAGTAAG CAAAAGGTTA TTATTGACCT TAAAGAAGAA 
CTATTTACAG TTATAAGTGA GAGAAACAGA CTTCGGGAAG AATTAGATAA TGTGTCAAAA 
GAAAGCAAAG CACTGGATCA GATGTCTAAA AAGATGAAAG AGAAAATAGA AGAGCTGGAG 
TCTCACCAAA GGGAGAGCCT CCGTCACATT GGGGCAGTAG AGTCTGAGGT CAAGGACAAA 
GCAGATCTTA TTCAGACTCT GTCCTTTAAT GTGGGTGAGC TAACAAAAGA CAAAGCTCAT 
CTCCAGGAGC AGCTGCAGAA TTTGCAGAAT GACTCACAAG AATTATCTTT GGCGATTGGT 
GAGCTGGAAA TACAAATTGG ACAACTGAAT AAAGAGAAAG AATCACTGGT CAAGGAGTCT 
CAGAACTTCC AGATCAAGCT GACTGAGTCA GAGTGTGAAA AGCAGACGAT CTCTAAGGCC 
TTGGAGGTGG CACTCAAGGA GAAAGGTGAG TTTGCAGTGC AGCTGAGCTC AGCCCAGGAG 
GAGGTGCATC AGCTGAGACG AGGCATTGAG AAACTGAGCG TCCGCATTGA GGCCGATGAG 
AAGAAGCACC TCAGTGCTGT GGCGAAGCTG AAAGAAAGCC AGCGTGAAAG CGACTCATTG 
AAGGATACAG TGGAGACTCT GGAGCGGGAA CTGGAGAGGT CAGAAGAAAA CCAAGAGCTG 
GCAATTCTTG ATTCTGAGAA TTTGAAAGCA GAGGTGGAGA CCCTTAAGGC ACAAAAGGAT 
GAAATGACCA AAAGCCTGAG AATTTTCGAA TTAGACCTTG TTACAGTTAG GACTGAAAGA 
GAAAATCTAG CAAAGCAGCT ACAAGAGAAA CAAAGTCGAG TGTCAGAATT AGATGAACGG 
TGCTCTTCCT TGAGAAGACT GTTGGAAGAG AAGGAGCAAG CAAGAGTACA GATGGAAGAA 
GACTCTAAGT CTGCAATGCT GATGCTTCAG ATGCAGTTAA AAGAACTCAG GGAGGAAGTG 
GCAGCCTTGT GTAATGACCA AGAAACCTTG AAGGCCCAAG AACAGAGTCT AGACCAACCA 
GGGGAGGAAG TGCATCATTT GAAAAGTAGC ATTCGAAAGC TCAAAGTTCA CATAGATGCT 
GATGAAAAGA AGCATCAAAA CATCCTAGAA CAACTGAAGG AAAGTAAGCA CCATGCAGAC 
TTGCTTAAGG ACCGAGTTGA GAACCTTGAA CAAGAATTGA TACTATCAGA GAAAAACATG 
ATTTTCCAAG CTGAAAAGTC CAAAGCAGAG ATACAGACTT TAAAATCAGA AATTCAAAGA 
ATGGCCCAAA ACCTCCAAGA CTTGCAGTTA GAACTTATTA GTACAAGGTC AGAAAATGAA 
AATCTCATGA AAGAATTAAA AAAAGAGCAA GAGCGAGTAT CTGACTTAGA AACAATAAAT 
TCTTCTATTG AAAACTTACT GAAAGATAAA GAGCAAGAAA AAGTACAGAT GAAAGAGGAA 
GCCAAAATAA CAGTGGAGAT GCTTCAAACT CAATTAAAGG AGCTAAACGA GACAGTGGTT 
TCCTTGTGCA ATGACCAAGA GGTCTCTAAG ACCAAAGAAC AGAATCTGGG TAGTCAAGTA 
CAAACTCTTG AACTTGAGAA GGCTCAGCTG CTACAGGACC TTGGTGAGGC CAAGAATAAA 
TATATTATTT TTCAGTCATC TGTAAATGCC CTCACTCAAG AAGTAGAAGC TGGCAAACAG 
AAACTAGAGA AGGGGGAGAA AGAGATCAGG ACACTGAAAG AGCAACTTAA AAGTCAGGAG 
CAGCTTGTGT GTAAACTTGC CCAAGTGGAA GGAGAGCAGC AACTCTGGCA GAAGCAGAAA 
CTAGAGCTGA GAAATGTGAC TATGGCACTG GAGCAGAAGG TCCAAGTGCT GCAATCTGAA 
AACAACACGT TGCAGAGCAC CTATGAAGCA CTGCAGAATT CCCACAAGAG TTTAGAGAGT 
GAACTTGGAT TGATAAAGTT GGAGAAAGTA GCGCTTGTTG AAAGAGTTAG CACAATATCT 
GGGAAGGAAG CAGAGCTGCA GAGGGAGCTG CGAGATATGC TACAGAAAAC AACACAGCTG 
AGCGAAGACT ACAATAAAGA GAAAAACAGG CTAACAGAAG AAGTGGAAGT GCTGCGTGAA 
GAACTGCAGA ACACCAAAGC AGCGCACCTG AAATCTGTGA ATCAACTTGA GAAGGAACTT 
CAGCGTGCTC AGGGGAAAAT AAAGTTGATG CTCAAATCCT GTAGACAGCT GGAAGGAGAA 
AAGGAGATGC TGCAGAAGGA GCTCTCCCAG CTTGAAGCTG CACAGCAGCA GAGAGCAGGT 
TCTCTTGTAG ACAGTAACGT AGATGAAGTA ATGACTGAGA ACAAAGCGCT GAAAGAGACT 
CTGGAAGAAA AAGTCAAGGA AGCAGATAAG TACTTGGATA AGTACTGTTC CCTGCTGATA 
AGCCACGAGG AGCTCGAGAA AGCCAAGGAG ATATTAGAAA TAGAAGTTGC TCGGCTGAAG 
TCACGGCAGT CCAGACAGGA TCTCCAGAGT TCTCCTTTGC TTAATTCTTC CATTCCAGGA 
CCGTCTCCAA ATACTTCTGT TAGTGAGATG AAGTCAGCAT CTGGCCAAAA TAAAGCTTCA 
GGCAAGAGGC AAAGGTCCAG TGGGATTTGG GAGCATGGTA AACGGGCAGC ACCTTCTACA 
GCAGAGACAT TTTCTAAGAA AAGCAGGAAG TCGGACAGTA AGAGCACTCG CCCTGCTGAG 
CACGAGCAGG AAACCGAGTT TGAGCCAGAA GGCCTCCCAG AAGTCGTTAA AAAAGGGTTT 
GCTGACATCC CAACTGGAAA GACAAGCCCA TATATCCTTC GGAGAACAAC CATGGCAACC 
AGGACCAGCC CCCGCTTTGC TACACAGAAG TTAGTGGGAT CTTCCCCATC TCTGGGCAAA 
GAAAATGTTG TAGAGTCCTC CAAACCAACA GCTGGTGGCA GCAGATCACA AAAGGTCAAG 
GTTGTTCAGG AGAGCTCAGC GGATTCACAC ACTGCCTTCC AAGAACTCCC AGCAAAATCT 
CTCACAGCCA GTAATATTCC TGGGAGAAAC TCTACAGAGA GCCCCAGGGA GGGCCTGAGG 
GCCAAGCGGG CCTACCCTGC CTCCAGCCCA GCTGCTGGGC CTGATCCCAC GAACAACGAA 
AACTGCCGGG TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497182.1
CDS	597..9374
Translation	MEICEHLEKSRQKLSHELQVKESQVNLQESQLSSCKKQIEKLEQELKRCKSEFERSQQVAQSADVSLNPCSTPQKLFATPLTPSSTYEDLKEKYNKEVEERKRLEEEVKALHAKKVSLPVSQATMNHRDIARHQASSSVFPWQQENTPSRLSSDALKTPLRRDGSAAHFLGEEVSPNKSSMKTGRGDCSSLPGEPHSAQLLHQAKAQNQDLKSKMTELELRLQGQEKEMRSQVNKCQDLQLQLEKTKVELIEKERILNKTRDEVVRSTAQYDQAAAKCTTLEQKLKTLTEELSCHRQNAESAKRSLEQRIKEKEKELQEELSRQHQSFQALDSEYTQMKTRLTQELQQVKHLHSTLQLELEKVTSVKQQLERNLEEIRLKLSRAEQALQASQVAENELRRSSEEMKKENSLIRSQSEQRTREVCHLEEELGKVKVSLSKSQNFAEEMKAKNTSQEIMLRDLQEKLNQQENSLTLEKLKLALADLERQRNCSQDLLKKREHHIDQLNNKLNKIEKEFETLLSALELKKKECEELKEEKNQISFWKIDSEKLINQIESEKEILLGKINHLETSLKTQQVSPDSNERIRTLEMERENFTVEIKNLQSMLDSKMVEIKTQKQAYLELQQKSESSDQKHQKEIENMCLKANKLTGQVESLECKLQLLSSEVVTKDQQYQDLRMEYETLRDLLKSRGSSLVTNEDNQRSSEDNQRSSEDNQRGSLAFEQQPAVSDSFANVMGRKGSINSERSDCSVDGGRSPEHIAILQNRVTSLESSLESQNQMNSDLQMRCEELLQIKGEVEENLSKAEQIHQNFVAETNQCISKLQEDAAVHQNIVAETLATLESKEKELQLLKEKLEAQQTEVQKLNKNNCLLEGTLKELQLLSDTLSSEKKEMNSIISLSKKNIEELTQANEALKEVNEALEQEKMNLLQKHEKITSCIAEQERSIAELSDQYKQERLQLLQRCEETEAVLEDLRGNYKTAQENNAKLECMLSECTALCENRKNELEQLKETFAKEQQEFLTKLAFAEEQNRKLMLELEIEQQTVRSEITNTNKHSMSATDGLRQECLTLNEEQNEQQNEVSNLTHENEQLMELTQTKHDSYLAVEPVENSVKATEDEIGKSSSQYQMDIDTKDISLDSYKAQLVHLEALVRILEVQLDQSEEENKKLHLELQTIREELETKSSQDPQSQARTGLKDCDTAEEKYVSMLQELSASQNENAHLQCSLQTAVNKLNELGKMCDVLRVEKLQLESELNDSRTECITATSQMTAEVEKLVSEMKMLNHESALSQNELMKDTSGGEFHDKANHSSVFLTPLDSSNFCEQMTLSSKEVRVHFAELQEKFSCLQSEHKILHDQHCEVSSKMSALRSYVDTLKAENSALSMSLRTLQGDLVKEGEPAAEGGHGLPLSFCGADSPSLTNFGETSFYKDVLEQTGDTCHLSLEGNASANSCDLDEEFSSSLEEETLTEKESPPAPGRTVEGLEVLCQVYLQSLKNLEEKTESQRIMKNKEIEKLEQLLSSERKELSCLRKQYLSEKEQWQQKLTSVTLEMESKLAEEKQQTKTLSLELEVARLQLQELDLSSRSLLGTDLESVVRCQNDNYDIKESEVYISETTEKTPKQDTDQTCDKDIQQDLGLETSVTESETTRLTGEGCEEQPPKTNCEAPAEDKTQDCSECISELCSSSNVLVPMDVLEDQGSIQNLQLQKDTLNENLRLLPEVEDWDKKVESLLNEIMEADSKLSLQEVQLKMKIATCIQLEKIVKDLRKEKADLSEKLESLPCNQEVCLRVERSEEDLGFNLDMGANELLSKSTKDNATNTEDNYKEKFLDMERELTRIKSEKANIEHHILSVETNLEVVQAEKLCLERDTESKQKVIIDLKEELFTVISERNRLREELDNVSKESKALDQMSKKMKEKIEELESHQRESLRHIGAVESEVKDKADLIQTLSFNVGELTKDKAHLQEQLQNLQNDSQELSLAIGELEIQIGQLNKEKESLVKESQNFQIKLTESECEKQTISKALEVALKEKGEFAVQLSSAQEEVHQLRRGIEKLSVRIEADEKKHLSAVAKLKESQRESDSLKDTVETLERELERSEENQELAILDSENLKAEVETLKAQKDEMTKSLRIFELDLVTVRTERENLAKQLQEKQSRVSELDERCSSLRRLLEEKEQARVQMEEDSKSAMLMLQMQLKELREEVAALCNDQETLKAQEQSLDQPGEEVHHLKSSIRKLKVHIDADEKKHQNILEQLKESKHHADLLKDRVENLEQELILSEKNMIFQAEKSKAEIQTLKSEIQRMAQNLQDLQLELISTRSENENLMKELKKEQERVSDLETINSSIENLLKDKEQEKVQMKEEAKITVEMLQTQLKELNETVVSLCNDQEVSKTKEQNLGSQVQTLELEKAQLLQDLGEAKNKYIIFQSSVNALTQEVEAGKQKLEKGEKEIRTLKEQLKSQEQLVCKLAQVEGEQQLWQKQKLELRNVTMALEQKVQVLQSENNTLQSTYEALQNSHKSLESELGLIKLEKVALVERVSTISGKEAELQRELRDMLQKTTQLSEDYNKEKNRLTEEVEVLREELQNTKAAHLKSVNQLEKELQRAQGKIKLMLKSCRQLEGEKEMLQKELSQLEAAQQQRAGSLVDSNVDEVMTENKALKETLEEKVKEADKYLDKYCSLLISHEELEKAKEILEIEVARLKSRQSRQDLQSSPLLNSSIPGPSPNTSVSEMKSASGQNKASGKRQRSSGIWEHGKRAAPSTAETFSKKSRKSDSKSTRPAEHEQETEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRFATQKLVGSSPSLGKENVVESSKPTAGGSRSQKVKVVQESSADSHTAFQELPAKSLTASNIPGRNSTESPREGLRAKRAYPASSPAAGPDPTNNENCRVQ

Target ORF information:

RefSeq Version	XM_006497119.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus centromere protein F (Cenpf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497119.3

ORF Insert Sequence:

ATGGAGATAT GCGAACATTT GGAGAAGTCA AGGCAGAAGC TGTCTCATGA ACTTCAAGTT 
AAGGAGTCAC AAGTGAATCT CCAAGAGAGC CAACTGAGCT CATGCAAAAA GCAAATAGAA 
AAACTGGAAC AGGAACTTAA GCGGTGTAAA TCTGAATTTG AAAGAAGCCA ACAAGTTGCA 
CAATCGGCAG ATGTTTCTCT GAATCCATGC AGTACACCAC AGAAACTCTT TGCAACTCCA 
CTCACACCGA GTTCCACATA CGAAGATCTG AAAGAAAAAT ATAATAAAGA AGTTGAAGAA 
CGGAAGAGGT TAGAGGAAGA GGTTAAAGCT TTGCATGCAA AAAAAGTGAG CCTGCCTGTT 
TCCCAAGCCA CCATGAACCA CCGGGACATT GCGAGACATC AGGCTTCCTC ATCAGTGTTT 
CCTTGGCAAC AGGAAAATAC CCCAAGTCGC CTTTCATCGG ATGCTCTGAA AACCCCACTG 
AGGAGAGACG GCTCTGCTGC TCACTTTTTG GGGGAAGAAG TGAGTCCTAA CAAATCAAGT 
ATGAAGACAG GGAGAGGAGA CTGCAGCAGC CTCCCTGGTG AGCCTCACAG CGCTCAGCTT 
TTGCACCAGG CCAAAGCCCA GAATCAAGAC CTAAAAAGCA AGATGACTGA GTTAGAACTA 
CGCCTGCAAG GGCAAGAAAA GGAAATGAGA AGCCAAGTGA ATAAATGTCA AGACTTACAG 
CTACAGCTGG AGAAGACGAA AGTGGAATTG ATTGAAAAGG AGAGAATTTT GAATAAAACC 
AGAGATGAAG TAGTGAGAAG CACAGCACAG TATGACCAGG CCGCAGCCAA GTGTACTACC 
TTGGAACAAA AGCTGAAAAC TTTGACTGAG GAGTTGAGTT GTCACCGACA GAACGCAGAG 
AGTGCTAAAC GTTCTCTGGA ACAGAGGATT AAGGAGAAAG AAAAGGAGCT TCAAGAGGAG 
CTGTCCCGAC AGCATCAATC TTTCCAAGCT CTGGACAGTG AGTACACTCA GATGAAAACC 
AGACTTACCC AGGAGTTACA GCAAGTCAAG CATTTGCACA GCACCCTCCA GCTGGAACTG 
GAGAAGGTCA CATCAGTGAA GCAGCAGTTA GAAAGGAATT TGGAAGAAAT TAGGCTTAAG 
TTGAGCAGAG CAGAACAAGC TCTTCAGGCA AGTCAGGTCG CAGAAAACGA GCTGAGGAGA 
AGCAGTGAGG AAATGAAGAA GGAGAACAGT CTCATTAGGA GTCAGTCTGA GCAGAGGACC 
AGAGAAGTCT GCCACCTGGA GGAAGAACTT GGTAAAGTCA AAGTGTCTTT GAGTAAGAGC 
CAGAATTTTG CAGAAGAAAT GAAGGCTAAG AATACCTCTC AGGAAATCAT GTTACGAGAT 
CTTCAGGAAA AACTAAATCA GCAAGAAAAC TCACTAACTT TAGAGAAGCT GAAACTTGCC 
CTAGCTGATC TGGAAAGACA GCGAAACTGT TCTCAAGATC TCTTGAAGAA AAGGGAACAT 
CACATTGATC AACTGAATAA TAAGTTAAAT AAGATAGAGA AAGAGTTTGA AACTTTGCTG 
AGTGCTTTGG AATTAAAAAA GAAAGAATGT GAAGAATTGA AAGAAGAGAA AAATCAGATT 
TCTTTTTGGA AAATTGATAG TGAAAAACTC ATAAATCAGA TAGAATCAGA AAAAGAAATC 
TTATTGGGTA AAATTAACCA CTTAGAGACC AGCCTCAAGA CACAACAAGT AAGTCCTGAC 
TCTAATGAGA GAATAAGAAC ACTGGAGATG GAAAGAGAAA ACTTTACTGT GGAGATTAAA 
AACCTTCAAA GTATGTTAGA CAGCAAGATG GTAGAGATCA AGACACAGAA ACAAGCTTAC 
TTGGAACTGC AGCAGAAATC CGAATCCTCG GACCAAAAGC ATCAGAAGGA GATAGAGAAT 
ATGTGCTTGA AAGCAAATAA GCTCACTGGG CAAGTTGAAA GTTTGGAATG TAAGCTTCAG 
TTATTGTCAA GTGAAGTAGT GACCAAAGAC CAGCAGTACC AAGACTTGCG TATGGAATAT 
GAGACACTGA GGGATTTGCT CAAGTCCAGA GGATCTTCTC TGGTGACAAA TGAGGATAAT 
CAGCGAAGTT CTGAGGATAA TCAGAGAAGT TCTGAGGATA ATCAGAGAGG CTCTTTGGCT 
TTTGAACAGC AGCCTGCAGT GAGTGATTCC TTTGCAAATG TAATGGGGAG AAAAGGAAGC 
ATAAATTCAG AAAGGAGTGA CTGCTCTGTA GATGGGGGCC GAAGTCCAGA ACATATAGCC 
ATCTTACAAA ATAGAGTCAC TTCACTTGAA AGTTCCTTGG AGTCTCAAAA CCAGATGAAT 
TCAGATTTGC AAATGCGGTG TGAAGAGTTG CTACAAATCA AAGGGGAAGT AGAAGAAAAC 
CTCAGTAAAG CAGAGCAGAT TCATCAGAAT TTTGTGGCTG AAACAAATCA ATGTATTAGT 
AAATTGCAGG AAGATGCTGC AGTTCATCAG AATATTGTTG CTGAGACTTT AGCAACCCTT 
GAGAGTAAGG AAAAAGAGTT ACAGCTTTTG AAAGAAAAAT TAGAAGCTCA GCAAACAGAG 
GTTCAAAAGT TAAATAAGAA TAACTGTCTT CTTGAAGGTA CTCTGAAGGA GCTACAGCTT 
TTATCTGACA CTCTGAGCTC AGAGAAGAAG GAAATGAATT CTATCATCTC ATTAAGTAAA 
AAAAACATTG AAGAGTTAAC CCAAGCAAAC GAGGCTCTCA AGGAAGTTAA TGAGGCCTTA 
GAGCAGGAGA AAATGAATTT ACTCCAAAAG CATGAGAAGA TTACAAGCTG TATAGCAGAA 
CAAGAGAGAA GCATTGCAGA GCTGTCTGAT CAGTACAAGC AAGAAAGACT TCAATTATTA 
CAACGGTGTG AAGAAACAGA AGCTGTGTTG GAAGATCTCA GGGGAAACTA CAAAACAGCA 
CAAGAAAACA ATGCTAAGTT AGAATGCATG CTCAGCGAGT GCACTGCTCT TTGTGAAAAT 
AGAAAAAATG AACTGGAGCA GTTAAAGGAA ACATTTGCAA AGGAACAGCA AGAATTCTTA 
ACAAAATTAG CTTTCGCTGA AGAGCAAAAC AGGAAACTAA TGCTAGAGTT GGAGATAGAG 
CAACAAACTG TGAGATCCGA GATTACAAAC ACCAACAAGC ATTCCATGAG TGCGACTGAT 
GGCTTAAGGC AAGAATGCTT GACTTTAAAC GAAGAGCAAA ATGAGCAGCA AAACGAAGTT 
AGCAACTTAA CACATGAGAA TGAGCAACTG ATGGAGTTAA CACAGACCAA ACATGATTCT 
TATCTCGCAG TAGAGCCAGT TGAGAACTCT GTAAAAGCAA CCGAAGATGA GATAGGTAAG 
AGTAGTTCCC AGTACCAGAT GGATATCGAC ACTAAAGACA TTTCTCTAGA TAGTTATAAG 
GCACAGCTGG TACATCTAGA AGCTTTGGTA AGAATTCTGG AAGTACAGCT TGACCAAAGT 
GAGGAGGAGA ACAAGAAGCT GCATCTGGAA TTACAGACGA TTAGAGAGGA GCTAGAAACC 
AAGAGTTCAC AGGACCCCCA GTCACAGGCA AGGACTGGGC TTAAAGACTG TGACACAGCA 
GAAGAAAAGT ATGTGTCCAT GCTACAGGAG TTGTCAGCAA GTCAAAACGA GAATGCACAC 
TTACAGTGCT CTCTACAGAC AGCAGTGAAC AAACTGAATG AGCTAGGGAA AATGTGTGAC 
GTATTGAGAG TTGAAAAGTT ACAGCTAGAG TCTGAGCTGA ATGACTCACG GACAGAGTGT 
ATCACGGCAA CTAGTCAGAT GACGGCAGAG GTCGAGAAGC TAGTGAGTGA AATGAAAATG 
CTAAACCACG AGAGTGCTCT GTCCCAGAAT GAGCTGATGA AGGACACCTC AGGTGGTGAA 
TTTCATGATA AAGCAAACCA CAGTTCTGTG TTCTTGACTC CTTTGGACAG TAGCAATTTC 
TGTGAACAGA TGACCTTGTC AAGCAAAGAG GTCCGAGTGC ACTTTGCTGA ATTACAGGAG 
AAATTCTCCT GTTTACAAAG TGAACACAAA ATTTTACATG ATCAGCACTG TGAGGTGAGC 
TCTAAGATGT CAGCACTGCG TTCCTACGTG GACACATTAA AAGCTGAAAA TTCTGCCTTG 
TCAATGAGTC TGAGAACCTT GCAGGGTGAC TTGGTAAAGG AGGGGGAGCC TGCAGCTGAG 
GGTGGGCATG GTCTGCCACT GTCGTTCTGT GGGGCAGACA GCCCGTCTCT GACAAATTTT 
GGAGAAACGT CCTTTTACAA AGACGTTTTA GAACAAACTG GAGACACATG TCATCTAAGT 
TTAGAAGGGA ACGCTTCAGC AAATTCTTGT GACTTGGATG AAGAGTTCTC CAGCAGTCTG 
GAGGAGGAGA CTCTGACTGA GAAGGAAAGC CCACCTGCCC CTGGGAGGAC TGTTGAGGGA 
CTTGAAGTCC TATGCCAGGT GTACTTGCAG TCCCTCAAGA ATCTAGAGGA GAAAACTGAG 
AGCCAAAGGA TTATGAAAAA TAAAGAAATT GAAAAGCTTG AGCAGTTACT GAGTTCTGAG 
AGGAAAGAGC TAAGCTGCCT TAGGAAGCAG TATTTGTCAG AAAAGGAGCA ATGGCAGCAG 
AAGCTAACAA GTGTCACTTT GGAAATGGAG TCCAAGTTAG CAGAGGAGAA GCAGCAGACC 
AAGACTCTGT CCCTTGAACT TGAGGTAGCA CGACTTCAGT TACAGGAGCT GGACCTGAGC 
TCCAGGTCTT TGCTTGGCAC TGACTTGGAA AGTGTTGTTC GGTGCCAAAA CGATAATTAT 
GATATAAAAG AATCAGAAGT ATATATTTCA GAGACTACAG AGAAAACACC AAAGCAGGAC 
ACTGACCAAA CTTGTGATAA AGATATTCAG CAGGACCTTG GTCTGGAAAC TTCAGTCACT 
GAGAGTGAGA CTACCAGGCT CACAGGAGAG GGGTGTGAAG AGCAGCCTCC GAAGACCAAT 
TGTGAGGCAC CAGCGGAGGA CAAAACCCAG GACTGCTCAG AATGCATTTC TGAATTGTGT 
TCTAGTTCCA ATGTTTTGGT GCCCATGGAT GTTCTGGAAG ATCAGGGGTC TATCCAGAAT 
CTCCAGTTGC AGAAAGACAC CTTAAATGAG AATTTGAGAT TACTTCCTGA AGTAGAGGAC 
TGGGACAAAA AAGTTGAAAG TTTGCTAAAT GAAATTATGG AGGCAGATTC AAAACTGAGT 
TTACAGGAAG TACAGCTCAA GATGAAGATT GCAACATGCA TACAATTGGA AAAAATAGTC 
AAGGACCTCA GAAAGGAAAA AGCTGACTTA AGTGAAAAGT TGGAATCCCT TCCATGTAAC 
CAGGAGGTAT GTCTGAGAGT AGAAAGGTCA GAAGAAGATC TTGGTTTTAA TTTAGATATG 
GGAGCAAATG AGTTGTTAAG TAAATCTACT AAAGATAATG CAACCAACAC AGAAGACAAT 
TATAAGGAGA AGTTTCTTGA TATGGAAAGA GAACTGACCA GAATTAAGTC TGAGAAAGCT 
AATATTGAGC ATCACATCCT ATCTGTGGAA ACTAACTTAG AGGTGGTTCA AGCAGAGAAG 
CTCTGTTTGG AAAGAGACAC TGAAAGTAAG CAAAAGGTTA TTATTGACCT TAAAGAAGAA 
CTATTTACAG TTATAAGTGA GAGAAACAGA CTTCGGGAAG AATTAGATAA TGTGTCAAAA 
GAAAGCAAAG CACTGGATCA GATGTCTAAA AAGATGAAAG AGAAAATAGA AGAGCTGGAG 
TCTCACCAAA GGGAGAGCCT CCGTCACATT GGGGCAGTAG AGTCTGAGGT CAAGGACAAA 
GCAGATCTTA TTCAGACTCT GTCCTTTAAT GTGGGTGAGC TAACAAAAGA CAAAGCTCAT 
CTCCAGGAGC AGCTGCAGAA TTTGCAGAAT GACTCACAAG AATTATCTTT GGCGATTGGT 
GAGCTGGAAA TACAAATTGG ACAACTGAAT AAAGAGAAAG AATCACTGGT CAAGGAGTCT 
CAGAACTTCC AGATCAAGCT GACTGAGTCA GAGTGTGAAA AGCAGACGAT CTCTAAGGCC 
TTGGAGGTGG CACTCAAGGA GAAAGGTGAG TTTGCAGTGC AGCTGAGCTC AGCCCAGGAG 
GAGGTGCATC AGCTGAGACG AGGCATTGAG AAACTGAGCG TCCGCATTGA GGCCGATGAG 
AAGAAGCACC TCAGTGCTGT GGCGAAGCTG AAAGAAAGCC AGCGTGAAAG CGACTCATTG 
AAGGATACAG TGGAGACTCT GGAGCGGGAA CTGGAGAGGT CAGAAGAAAA CCAAGAGCTG 
GCAATTCTTG ATTCTGAGAA TTTGAAAGCA GAGGTGGAGA CCCTTAAGGC ACAAAAGGAT 
GAAATGACCA AAAGCCTGAG AATTTTCGAA TTAGACCTTG TTACAGTTAG GACTGAAAGA 
GAAAATCTAG CAAAGCAGCT ACAAGAGAAA CAAAGTCGAG TGTCAGAATT AGATGAACGG 
TGCTCTTCCT TGAGAAGACT GTTGGAAGAG AAGGAGCAAG CAAGAGTACA GATGGAAGAA 
GACTCTAAGT CTGCAATGCT GATGCTTCAG ATGCAGTTAA AAGAACTCAG GGAGGAAGTG 
GCAGCCTTGT GTAATGACCA AGAAACCTTG AAGGCCCAAG AACAGAGTCT AGACCAACCA 
GGGGAGGAAG TGCATCATTT GAAAAGTAGC ATTCGAAAGC TCAAAGTTCA CATAGATGCT 
GATGAAAAGA AGCATCAAAA CATCCTAGAA CAACTGAAGG AAAGTAAGCA CCATGCAGAC 
TTGCTTAAGG ACCGAGTTGA GAACCTTGAA CAAGAATTGA TACTATCAGA GAAAAACATG 
ATTTTCCAAG CTGAAAAGTC CAAAGCAGAG ATACAGACTT TAAAATCAGA AATTCAAAGA 
ATGGCCCAAA ACCTCCAAGA CTTGCAGTTA GAACTTATTA GTACAAGGTC AGAAAATGAA 
AATCTCATGA AAGAATTAAA AAAAGAGCAA GAGCGAGTAT CTGACTTAGA AACAATAAAT 
TCTTCTATTG AAAACTTACT GAAAGATAAA GAGCAAGAAA AAGTACAGAT GAAAGAGGAA 
GCCAAAATAA CAGTGGAGAT GCTTCAAACT CAATTAAAGG AGCTAAACGA GACAGTGGTT 
TCCTTGTGCA ATGACCAAGA GGTCTCTAAG ACCAAAGAAC AGAATCTGGG TAGTCAAGTA 
CAAACTCTTG AACTTGAGAA GGCTCAGCTG CTACAGGACC TTGGTGAGGC CAAGAATAAA 
TATATTATTT TTCAGTCATC TGTAAATGCC CTCACTCAAG AAGTAGAAGC TGGCAAACAG 
AAACTAGAGA AGGGGGAGAA AGAGATCAGG ACACTGAAAG AGCAACTTAA AAGTCAGGAG 
CAGCTTGTGT GTAAACTTGC CCAAGTGGAA GGAGAGCAGC AACTCTGGCA GAAGCAGAAA 
CTAGAGCTGA GAAATGTGAC TATGGCACTG GAGCAGAAGG TCCAAGTGCT GCAATCTGAA 
AACAACACGT TGCAGAGCAC CTATGAAGCA CTGCAGAATT CCCACAAGAG TTTAGAGAGT 
GAACTTGGAT TGATAAAGTT GGAGAAAGTA GCGCTTGTTG AAAGAGTTAG CACAATATCT 
GGGAAGGAAG CAGAGCTGCA GAGGGAGCTG CGAGATATGC TACAGAAAAC AACACAGCTG 
AGCGAAGACT ACAATAAAGA GAAAAACAGG CTAACAGAAG AAGTGGAAGT GCTGCGTGAA 
GAACTGCAGA ACACCAAAGC AGCGCACCTG AAATCTGTGA ATCAACTTGA GAAGGAACTT 
CAGCGTGCTC AGGGGAAAAT AAAGTTGATG CTCAAATCCT GTAGACAGCT GGAAGGAGAA 
AAGGAGATGC TGCAGAAGGA GCTCTCCCAG CTTGAAGCTG CACAGCAGCA GAGAGCAGGT 
TCTCTTGTAG ACAGTAACGT AGATGAAGTA ATGACTGAGA ACAAAGCGCT GAAAGAGACT 
CTGGAAGAAA AAGTCAAGGA AGCAGATAAG TACTTGGATA AGTACTGTTC CCTGCTGATA 
AGCCACGAGG AGCTCGAGAA AGCCAAGGAG ATATTAGAAA TAGAAGTTGC TCGGCTGAAG 
TCACGGCAGT CCAGACAGGA TCTCCAGAGT TCTCCTTTGC TTAATTCTTC CATTCCAGGA 
CCGTCTCCAA ATACTTCTGT TAGTGAGATG AAGTCAGCAT CTGGCCAAAA TAAAGCTTCA 
GGCAAGAGGC AAAGGTCCAG TGGGATTTGG GAGCATGGTA AACGGGCAGC ACCTTCTACA 
GCAGAGACAT TTTCTAAGAA AAGCAGGAAG TCGGACAGTA AGAGCACTCG CCCTGCTGAG 
CACGAGCAGG AAACCGAGTT TGAGCCAGAA GGCCTCCCAG AAGTCGTTAA AAAAGGGTTT 
GCTGACATCC CAACTGGAAA GACAAGCCCA TATATCCTTC GGAGAACAAC CATGGCAACC 
AGGACCAGCC CCCGCTTTGC TACACAGAAG TTAGTGGGAT CTTCCCCATC TCTGGGCAAA 
GAAAATGTTG TAGAGTCCTC CAAACCAACA GCTGGTGGCA GCAGATCACA AAAGGTCAAG 
GTTGTTCAGG AGAGCTCAGC GGATTCACAC ACTGCCTTCC AAGAACTCCC AGCAAAATCT 
CTCACAGCCA GTAATATTCC TGGGAGAAAC TCTACAGAGA GCCCCAGGGA GGGCCTGAGG 
GCCAAGCGGG CCTACCCTGC CTCCAGCCCA GCTGCTGGGC CTGATCCCAC GAACAACGAA 
AACTGCCGGG TCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities.
Molecular biology of the cell27(13)1990-9(2016 07)
Pfaltzgraff ER,Roth GM,Miller PM,Gintzig AG,Ohi R,Bader DM

Predicting Drug Response in Human Prostate Cancer from Preclinical Analysis of In Vivo Mouse Models.
Cell reports12(12)2060-71(2015 Sep)
Mitrofanova A,Aytes A,Zou M,Shen MM,Abate-Shen C,Califano A

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Cenpf cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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