Sf3b4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sf3b4
Entrez Gene ID	107701
Full Name	splicing factor 3b, subunit 4
Synonyms	SF3b49,SF3b50,Sap49
General protein information	Preferred Names splicing factor 3b, subunit 4 Names splicing factor 3B subunit 4 spliceosome associated protein 49 splicing factor 3b, subunit 4, 49kD
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.1
Summary	This gene encodes one of the subunits of splicing factor 3B. A similar gene in human encodes a protein that cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes, and also belongs with the minor U12-dependent spliceosome. [provided by RefSeq, May 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_153053.4	NP_694693.1	splicing factor 3B subunit 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	SF3B4	NP_005841.1
Pan troglodytes (chimpanzee)	SF3B4	XP_513768.2
Mus musculus (house mouse)	Sf3b4	NP_694693.1
	SF3B4	XP_001097793.1
Bos taurus (cattle)	SF3B4	NP_001192513.1
Canis lupus familiaris (dog)	SF3B4	XP_540295.3
Rattus norvegicus (Norway rat)	Sf3b4	NP_001011951.1
Gallus gallus (chicken)	SF3B4	XP_423721.2
Danio rerio (zebrafish)	sf3b4	NP_705947.3

Gene Ontology
Bibliography

Related articles in PubMed

A broadly applicable high-throughput screening strategy identifies new regulators of Dlg4 (Psd-95) alternative splicing.
Zheng S, Damoiseaux R, Chen L, Black DL
Genome research23(6)998-1007(2013 Jun)

Mammalian polycomb-mediated repression of Hox genes requires the essential spliceosomal protein Sf3b1.
Isono K, Mizutani-Koseki Y, Komori T, Schmidt-Zachmann MS, Koseki H
Genes & development19(5)536-41(2005 Mar)

Developmental expression of the murine spliceosome-associated protein mSAP49.
Ruiz-Lozano P, Doevendans P, Brown A, Gruber PJ, Chien KR
Developmental dynamics : an official publication of the American Association of Anatomists208(4)482-90(1997 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sf3b4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sf3b4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02068	NM_153053.4 Latest version!	Mus musculus splicing factor 3b, subunit 4 (Sf3b4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sf3b4 ( NM_153053.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_694693.1 Mus musculus splicing factor 3b, subunit 4 (Sf3b4), mRNA.

Price & Availability↑

CloneID	OMu02068
Clone ID Related Accession (Same CDS sequence)	NM_153053.4
Accession Version	NM_153053.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	splicing factor 3B subunit 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ060196.1 and AK047751.1. On May 7, 2015 this sequence version replaced NM_153053.3. ##Evidence-Data-START## Transcript exon combination :: AK047751.1, BC026567.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCG GACCGATCTC CGAACGGAAT CAGGATGCCA CGGTGTACGT GGGCGGTCTG 
GACGAGAAAG TGAGTGAGCC GCTGCTGTGG GAGCTCTTTC TCCAGGCAGG GCCAGTGGTC 
AACACCCACA TGCCCAAGGA CAGAGTCACT GGCCAGCACC AGGGCTATGG CTTTGTTGAA 
TTCCTGAGCG AGGAAGATGC CGACTATGCC ATTAAGATTA TGAACATGAT CAAACTCTAT 
GGAAAGCCAA TACGGGTGAA CAAGGCCTCA GCTCACAACA AAAACCTGGA TGTGGGAGCC 
AACATTTTCA TTGGAAATCT GGACCCAGAA ATTGATGAAA AGCTGCTTTA CGATACTTTC 
AGCGCCTTTG GAGTCATCCT ACAGACCCCC AAGATCATGC GGGACCCTGA CACAGGCAAC 
TCCAAGGGTT ACGCCTTCAT TAATTTTGCC TCCTTCGATG CTTCGGATGC AGCAATTGAG 
GCCATGAACG GGCAGTACCT GTGTAACCGT CCTATCACTG TGTCTTATGC CTTCAAGAAG 
GACTCTAAGG GTGAACGCCA TGGCTCAGCA GCTGAAAGAC TCCTGGCAGC CCAGAACCCG 
CTGTCCCAGG CTGACCGCCC TCACCAGCTG TTTGCCGATG CACCCCCTCC GCCCTCTGCC 
CCCAATCCCG TGGTTTCATC CCTGGGTTCT GGGCTTCCTC CACCAGGCAT GCCGCCTCCT 
GGCTCTTTTC CACCTCCAGT GCCACCTCCT GGGGCCCTCC CTCCTGGGAT TCCCCCAGCC 
ATGCCCCCAC CACCTATGCC ACCTGGGGCT GGAGGACATG GCCCCCCAGC AGCAGGGACT 
CCAGGGGCTG GACATCCCGG TCACGGACAC TCACATCCTC ACCCGTTCCC ACCAGGTGGG 
ATGCCCCATC CAGGGATGTC CCAGATGCAG CTGGCCCACC ATGGCCCCCA TGGCCTAGGA 
CACCCCCATG CTGGGCCTCC GGGCTCTGGG GGGCAGCCAC CACCTCGGCC ACCTCCTGGA 
ATGCCTCATC CTGGACCTCC TCCAATGGGC ATGCCCCCCC GAGGGCCTCC TTTTGGATCT 
CCCATGGGTC ACCCAGGTCC CATGCCTCCA CACGGCATGC GTGGGCCTCC TCCATTGATG 
CCCCCTCATG GATACACGGG TCCTCCAAGA CCCCCTCCCT ATGGCTACCA GCGGGGGCCC 
CTCCCGCCAC CCAGACCCAC TCCACGGCCC CCAGTTCCAC CTCGTGGTCC TCTTCGGGGC 
CCACTTCCTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694693.1
CDS	69..1343
Translation	MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQLFADAPPPPSAPNPVVSSLGSGLPPPGMPPPGSFPPPVPPPGALPPGIPPAMPPPPMPPGAGGHGPPAAGTPGAGHPGHGHSHPHPFPPGGMPHPGMSQMQLAHHGPHGLGHPHAGPPGSGGQPPPRPPPGMPHPGPPPMGMPPRGPPFGSPMGHPGPMPPHGMRGPPPLMPPHGYTGPPRPPPYGYQRGPLPPPRPTPRPPVPPRGPLRGPLPQ

Target ORF information:

RefSeq Version	NM_153053.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus splicing factor 3b, subunit 4 (Sf3b4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153053.4

ORF Insert Sequence:

ATGGCTGCCG GACCGATCTC CGAACGGAAT CAGGATGCCA CGGTGTACGT GGGCGGTCTG 
GACGAGAAAG TGAGTGAGCC GCTGCTGTGG GAGCTCTTTC TCCAGGCAGG GCCAGTGGTC 
AACACCCACA TGCCCAAGGA CAGAGTCACT GGCCAGCACC AGGGCTATGG CTTTGTTGAA 
TTCCTGAGCG AGGAAGATGC CGACTATGCC ATTAAGATTA TGAACATGAT CAAACTCTAT 
GGAAAGCCAA TACGGGTGAA CAAGGCCTCA GCTCACAACA AAAACCTGGA TGTGGGAGCC 
AACATTTTCA TTGGAAATCT GGACCCAGAA ATTGATGAAA AGCTGCTTTA CGATACTTTC 
AGCGCCTTTG GAGTCATCCT ACAGACCCCC AAGATCATGC GGGACCCTGA CACAGGCAAC 
TCCAAGGGTT ACGCCTTCAT TAATTTTGCC TCCTTCGATG CTTCGGATGC AGCAATTGAG 
GCCATGAACG GGCAGTACCT GTGTAACCGT CCTATCACTG TGTCTTATGC CTTCAAGAAG 
GACTCTAAGG GTGAACGCCA TGGCTCAGCA GCTGAAAGAC TCCTGGCAGC CCAGAACCCG 
CTGTCCCAGG CTGACCGCCC TCACCAGCTG TTTGCCGATG CACCCCCTCC GCCCTCTGCC 
CCCAATCCCG TGGTTTCATC CCTGGGTTCT GGGCTTCCTC CACCAGGCAT GCCGCCTCCT 
GGCTCTTTTC CACCTCCAGT GCCACCTCCT GGGGCCCTCC CTCCTGGGAT TCCCCCAGCC 
ATGCCCCCAC CACCTATGCC ACCTGGGGCT GGAGGACATG GCCCCCCAGC AGCAGGGACT 
CCAGGGGCTG GACATCCCGG TCACGGACAC TCACATCCTC ACCCGTTCCC ACCAGGTGGG 
ATGCCCCATC CAGGGATGTC CCAGATGCAG CTGGCCCACC ATGGCCCCCA TGGCCTAGGA 
CACCCCCATG CTGGGCCTCC GGGCTCTGGG GGGCAGCCAC CACCTCGGCC ACCTCCTGGA 
ATGCCTCATC CTGGACCTCC TCCAATGGGC ATGCCCCCCC GAGGGCCTCC TTTTGGATCT 
CCCATGGGTC ACCCAGGTCC CATGCCTCCA CACGGCATGC GTGGGCCTCC TCCATTGATG 
CCCCCTCATG GATACACGGG TCCTCCAAGA CCCCCTCCCT ATGGCTACCA GCGGGGGCCC 
CTCCCGCCAC CCAGACCCAC TCCACGGCCC CCAGTTCCAC CTCGTGGTCC TCTTCGGGGC 
CCACTTCCTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A broadly applicable high-throughput screening strategy identifies new regulators of Dlg4 (Psd-95) alternative splicing.
Genome research23(6)998-1007(2013 Jun)
Zheng S,Damoiseaux R,Chen L,Black DL

Mammalian polycomb-mediated repression of Hox genes requires the essential spliceosomal protein Sf3b1.
Genes & development19(5)536-41(2005 Mar)
Isono K,Mizutani-Koseki Y,Komori T,Schmidt-Zachmann MS,Koseki H

Developmental expression of the murine spliceosome-associated protein mSAP49.
Developmental dynamics : an official publication of the American Association of Anatomists208(4)482-90(1997 Apr)
Ruiz-Lozano P,Doevendans P,Brown A,Gruber PJ,Chien KR

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Sf3b4 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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