Wwp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wwp1
Entrez Gene ID	107568
Full Name	WW domain containing E3 ubiquitin protein ligase 1
Synonyms	8030445B08Rik,AIP5,SDRP1,Tiul1
General protein information	Preferred Names WW domain containing E3 ubiquitin protein ligase 1 Names NEDD4-like E3 ubiquitin-protein ligase WWP1 HECT-type E3 ubiquitin transferase WWP1 WW domain-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001355222.1	NP_001342151.1	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform 3
XM_006537546.3	XP_006537609.1	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X2
NM_177327.5	NP_796301.2	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform 1
XM_006537546.4	XP_006537609.1	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
XM_006537545.3	XP_006537608.1	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
NM_001276292.1	NP_001263221.1	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform 2

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009074	XP_319824.3
Homo sapiens (human)	WWP1	NP_008944.1
Pan troglodytes (chimpanzee)	WWP1	XP_519843.2
Drosophila melanogaster (fruit fly)	Su(dx)	NP_722754.1
	WWP1	XP_001083173.1
Bos taurus (cattle)	WWP1	NP_001032540.1
Mus musculus (house mouse)	Wwp1	NP_796301.2
Rattus norvegicus (Norway rat)	Wwp1	NP_001019928.1
Caenorhabditis elegans (roundworm)	wwp-1	NP_740775.1
Xenopus tropicalis (tropical clawed frog)	wwp1	NP_001090844.1
Canis lupus familiaris (dog)	WWP1	XP_003640065.1
Gallus gallus (chicken)	WWP1	NP_001012572.1

Gene Ontology
Bibliography

Related articles in PubMed

Atypical ubiquitination by E3 ligase WWP1 inhibits the proteasome-mediated degradation of mutant huntingtin.
Lin L, Jin Z, Tan H, Xu Q, Peng T, Li H
Brain research1643103-12(2016 07)

Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis.
Basheer WA, Harris BS, Mentrup HL, Abreha M, Thames EL, Lea JB, Swing DA, Copeland NG, Jenkins NA, Price RL, Matesic LE
Journal of molecular and cellular cardiology881-13(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Atypical ubiquitination by E3 ligase WWP1 inhibits the proteasome-mediated degradation of mutant huntingtin. This suggests that the E3 ligase WWP1 is involved in the pathogenesis of Huntington's disease; therefore, it may be a novel target for therapeutic intervention.
Title: Atypical ubiquitination by E3 ligase WWP1 inhibits the proteasome-mediated degradation of mutant huntingtin.

Cardiomyocyte-specific overexpression of Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis.
Title: Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis.

Wwp1 negatively regulates osteoblast functions.
Title: Ubiquitin E3 ligase Wwp1 negatively regulates osteoblast function by inhibiting osteoblast differentiation and migration.

WWP1 down-regulates AMPKalpha2 under high glucose culture conditions via the ubiquitin-proteasome pathway
Title: E3 ubiquitin ligase, WWP1, interacts with AMPK?2 and down-regulates its expression in skeletal muscle C2C12 cells.

WWP1 functions as an E3 ligase when cells are stimulated with LPS by binding to TRAF6 and promoting K48-linked polyubiquitination. This results in the proteasomal degradation of TRAF6
Title: WW domain containing E3 ubiquitin protein ligase 1 (WWP1) negatively regulates TLR4-mediated TNF-? and IL-6 production by proteasomal degradation of TNF receptor associated factor 6 (TRAF6).

The following Wwp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wwp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13693	NM_001276292.1 Latest version!	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu14063	NM_177327.5 Latest version!	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu32681	NM_001355222.1 Latest version!	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu32681	XM_006537545.3 Latest version!	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu32682	XM_006537546.4 Latest version!	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu32682	XM_006537546.3	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wwp1 ( XM_006537546.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537609.1 Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu32682
Clone ID Related Accession (Same CDS sequence)	XM_006537546.3 , XM_006537546.4
Accession Version	XM_006537546.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537546.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTACTT TACTACCATC TGAAGATACC ACCTCGACTT CCAATTGCAC TAGCACCACC 
ACCCAAGAGC CTCCTGTACA AGAACCCCCA GCATCCTCAG AACACAGTGA ATGTATTCCC 
TCTGCCAGTG CGGAAGTGGG ACCAGAAGCT CGGAGCCTCA TAGACCCTGA CTCTGATTCT 
AGAAATAATT CTGTGTTTGA CAAAGTAAGG CAGCCAGAAG GGTGTGTAGA ACCTCTGCGG 
CCACAGTCTG GAAACACCAA CACAGAAGCT TTGCCATCAG GGTGGGAACA GAGAAAAGAT 
CCTCATGGTA GAACCTATTA TGTGGATCAT AATACTCGAA CTACTACATG GGAGAGACCA 
CAGCCTTTGC CTCCAGGTTG GGAAAGAAGA GTTGATGATC GTGGAAGAGT TTATTATGTG 
GATCATAACA CCAGAACAAC AACCTGGCAG CGGCCTACTA TGGAATCTGT TCGAAATTTT 
GAACAGTGGC AGTCTCAGCG GAATCAATTG CAGGGAGCTA TGCAACAATT CAACCAACGA 
TACCTCTATT CGGCTTCCAT GTTAGCTGCA GAAAATGACC CCTATGGACC CTTACCACCG 
GGCTGGGAAA AAAGAGTGGA TTCAACAGAC AGAGTTTACT TTGTGAATCA CAATACAAAA 
ACAACCCAGT GGGAAGATCC AAGAACTCAA GGCTTACCAA ATGAAGAACC CCTGCCCGAA 
GGCTGGGAAA TCAGGTATAC TCGGGAAGGT GTTAGGTACT TTGTTGATCA TAACACGAGA 
ACAACAACAT TCAAAGACCC TCGCAATGGG AAGTCATCAG TAACTAAAGG TGGTCCACAG 
ATTGCTTATG AACGCAGCTT TAGGTGGAAG CTTGCTCACT TCCGTTACTT GTGCCAGTCT 
AATGCACTAC CGAGTCATGT AAAGATCAAT GTGTCCCGAC AGACATTATT TGAAGATTCC 
TTCCAACAGA TTATGGCATT AAAACCATAT GACTTGAGGA GGCGATTATA TGTAATATTT 
AGAGGAGAAG AAGGTCTTGA TTATGGTGGC CTTGCAAGAG AATGGTTTTT CTTGCTTTCC 
CATGAAGTTT TGAACCCAAT GTATTGCTTA TTTGAGTATG CAGGCAAGAA TAACTATTGT 
TTACAGATAA ATCCAGCATC AACTATAAAT CCTGACCATC TTTCATACTT CTGTTTTATT 
GGTCGCTTTA TTGCAATGGC ACTGTTCCAT GGAAAGTTTA TTGATACTGG TTTCTCTTTA 
CCATTCTACA AGCGTATGCT AAGTAAAAAA CTTACTATTA AGGATTTGGA ATCTATTGAT 
ACTGAATTTT ACAACTCTCT AATCTGGATA AGAGATAACA ATATTGAAGA ATGTGGCTTA 
GAAATGTACT TTTCTGTAGA CATGGAGATT CTGGGAAAAG TTACTTCACA TGATTTAAAG 
TTGGGAGGTT CCAATATCCT GGTGACGGAA GAAAACAAAG ATGAATATAT TGGTTTAATG 
ACAGAATGGC GTTTTTCTCG AGGAGTACAA GAACAGACCA AAGCTTTCCT TGATGGGTTT 
AATGAAGTTG TTCCCCTTCA GTGGCTACAG TACTTTGATG AAAAAGAATT GGAGGTTATG 
CTGTGTGGCA TGCAAGAGGT TGACTTGGCA GACTGGCAGA GAAATACTGT TTATCGACAT 
TATACAAGAA ACAGCAAGCA AATTATTTGG TTTTGGCAGT TTGTGAAAGA GACTGATAAT 
GAAGTACGAA TGCGACTCTT GCAGTTTGTG ACTGGAACCT GTCGCTTGCC TCTAGGAGGA 
TTTGCTGAGC TCATGGGAAG TAATGGGCCT CAAAAGTTCT GTATTGAAAA AGTTGGCAAA 
GACACCTGGT TACCAAGAAG CCATACATGT TTTAATCGCT TGGATCTACC ACCATATAAG 
AGTTATGAAC AACTAAAGGA AAAACTTCTT TTTGCAATAG AAGAGACAGA GGGATTTGGA 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537609.1
CDS	808..2796
Translation	MGTLLPSEDTTSTSNCTSTTTQEPPVQEPPASSEHSECIPSASAEVGPEARSLIDPDSDSRNNSVFDKVRQPEGCVEPLRPQSGNTNTEALPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_006537546.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537546.3

ORF Insert Sequence:

ATGGGTACTT TACTACCATC TGAAGATACC ACCTCGACTT CCAATTGCAC TAGCACCACC 
ACCCAAGAGC CTCCTGTACA AGAACCCCCA GCATCCTCAG AACACAGTGA ATGTATTCCC 
TCTGCCAGTG CGGAAGTGGG ACCAGAAGCT CGGAGCCTCA TAGACCCTGA CTCTGATTCT 
AGAAATAATT CTGTGTTTGA CAAAGTAAGG CAGCCAGAAG GGTGTGTAGA ACCTCTGCGG 
CCACAGTCTG GAAACACCAA CACAGAAGCT TTGCCATCAG GGTGGGAACA GAGAAAAGAT 
CCTCATGGTA GAACCTATTA TGTGGATCAT AATACTCGAA CTACTACATG GGAGAGACCA 
CAGCCTTTGC CTCCAGGTTG GGAAAGAAGA GTTGATGATC GTGGAAGAGT TTATTATGTG 
GATCATAACA CCAGAACAAC AACCTGGCAG CGGCCTACTA TGGAATCTGT TCGAAATTTT 
GAACAGTGGC AGTCTCAGCG GAATCAATTG CAGGGAGCTA TGCAACAATT CAACCAACGA 
TACCTCTATT CGGCTTCCAT GTTAGCTGCA GAAAATGACC CCTATGGACC CTTACCACCG 
GGCTGGGAAA AAAGAGTGGA TTCAACAGAC AGAGTTTACT TTGTGAATCA CAATACAAAA 
ACAACCCAGT GGGAAGATCC AAGAACTCAA GGCTTACCAA ATGAAGAACC CCTGCCCGAA 
GGCTGGGAAA TCAGGTATAC TCGGGAAGGT GTTAGGTACT TTGTTGATCA TAACACGAGA 
ACAACAACAT TCAAAGACCC TCGCAATGGG AAGTCATCAG TAACTAAAGG TGGTCCACAG 
ATTGCTTATG AACGCAGCTT TAGGTGGAAG CTTGCTCACT TCCGTTACTT GTGCCAGTCT 
AATGCACTAC CGAGTCATGT AAAGATCAAT GTGTCCCGAC AGACATTATT TGAAGATTCC 
TTCCAACAGA TTATGGCATT AAAACCATAT GACTTGAGGA GGCGATTATA TGTAATATTT 
AGAGGAGAAG AAGGTCTTGA TTATGGTGGC CTTGCAAGAG AATGGTTTTT CTTGCTTTCC 
CATGAAGTTT TGAACCCAAT GTATTGCTTA TTTGAGTATG CAGGCAAGAA TAACTATTGT 
TTACAGATAA ATCCAGCATC AACTATAAAT CCTGACCATC TTTCATACTT CTGTTTTATT 
GGTCGCTTTA TTGCAATGGC ACTGTTCCAT GGAAAGTTTA TTGATACTGG TTTCTCTTTA 
CCATTCTACA AGCGTATGCT AAGTAAAAAA CTTACTATTA AGGATTTGGA ATCTATTGAT 
ACTGAATTTT ACAACTCTCT AATCTGGATA AGAGATAACA ATATTGAAGA ATGTGGCTTA 
GAAATGTACT TTTCTGTAGA CATGGAGATT CTGGGAAAAG TTACTTCACA TGATTTAAAG 
TTGGGAGGTT CCAATATCCT GGTGACGGAA GAAAACAAAG ATGAATATAT TGGTTTAATG 
ACAGAATGGC GTTTTTCTCG AGGAGTACAA GAACAGACCA AAGCTTTCCT TGATGGGTTT 
AATGAAGTTG TTCCCCTTCA GTGGCTACAG TACTTTGATG AAAAAGAATT GGAGGTTATG 
CTGTGTGGCA TGCAAGAGGT TGACTTGGCA GACTGGCAGA GAAATACTGT TTATCGACAT 
TATACAAGAA ACAGCAAGCA AATTATTTGG TTTTGGCAGT TTGTGAAAGA GACTGATAAT 
GAAGTACGAA TGCGACTCTT GCAGTTTGTG ACTGGAACCT GTCGCTTGCC TCTAGGAGGA 
TTTGCTGAGC TCATGGGAAG TAATGGGCCT CAAAAGTTCT GTATTGAAAA AGTTGGCAAA 
GACACCTGGT TACCAAGAAG CCATACATGT TTTAATCGCT TGGATCTACC ACCATATAAG 
AGTTATGAAC AACTAAAGGA AAAACTTCTT TTTGCAATAG AAGAGACAGA GGGATTTGGA 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wwp1 ( NM_001276292.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001263221.1 Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13693
Clone ID Related Accession (Same CDS sequence)	NM_001276292.1
Accession Version	NM_001276292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ101217.1, AK168567.1, AL683894.6, AK031762.1 and AK033138.1. Transcript Variant: This variant (2) has multiple coding region differences and initiates translation at a downstream start codon, compared to variant 1. The use of the start codon used in variant 1 would render the transcript a candidate for nonsense-mediated mRNA decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which is associated with a strong Kozak sequence. The resulting isoform (2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026829.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGACGAG CAGCTGATTG TAAATGTGAC CCCACAGACC ACCTTGGAGT TCCGAGTTTG 
GAGTCACCAC ACTTTAAAGG CAGATGCTCT ATTAGGAAAA GCAACAGTGG ACTTGAAACA 
AGTGCTGTTA ACACACAACA GAAAATGTTG CCTGTTGAAG GTACTATTGG AATTGATAAT 
CATGTATCAA CCAATACTGT TGTTCCCAAC TCCTGCTGTT CACATGTAGT TAATGGAGAA 
AACACACCTT CATCCCCGTC TCAGGTTGCT GCCAGACCCA AAAATGCTCC AGCTCCAAAG 
CCAGTCACAT CTGCGCCCAC CAGTGACACG GTTAATGGAG AATCATCATC TGTCCTAGCT 
GATAATACTT CAACCATGGG TACTTTACTA CCATCTGAAG ATACCACCTC GACTTCCAAT 
TGCACTAGCA CCACCACCCA AGAGCCTCCT GTACAAGAAC CCCCAGCATC CTCAGAACAC 
AGTGAATGTA TTCCCTCTGC CAGTGCGGAA GTGGGACCAG AAGCTCGGAG CCTCATAGAC 
CCTGACTCTG ATTCTAGAAA TAATTCTGTG TTTGACAAAG TAAGGCAGCC AGAAGGGTGT 
GTAGAACCTC TGCGGCCACA GTCTGGAAAC ACCAACACAG AAGCTTTGCC ATCAGGGTGG 
GAACAGAGAA AAGATCCTCA TGGTAGAACC TATTATGTGG ATCATAATAC TCGAACTACT 
ACATGGGAGA GACCACAGCC TTTGCCTCCA GGTTGGGAAA GAAGAGTTGA TGATCGTGGA 
AGAGTTTATT ATGTGGATCA TAACACCAGA ACAACAACCT GGCAGCGGCC TACTATGGAA 
TCTGTTCGAA ATTTTGAACA GTGGCAGTCT CAGCGGAATC AATTGCAGGG AGCTATGCAA 
CAATTCAACC AACGATACCT CTATTCGGCT TCCATGTTAG CTGCAGAAAA TGACCCCTAT 
GGACCCTTAC CACCGGGCTG GGAAAAAAGA GTGGATTCAA CAGACAGAGT TTACTTTGTG 
AATCACAATA CAAAAACAAC CCAGTGGGAA GATCCAAGAA CTCAAGGCTT ACCAAATGAA 
GAACCCCTGC CCGAAGGCTG GGAAATCAGG TATACTCGGG AAGGTGTTAG GTACTTTGTT 
GATCATAACA CGAGAACAAC AACATTCAAA GACCCTCGCA ATGGGAAGTC ATCAGTAACT 
AAAGGTGGTC CACAGATTGC TTATGAACGC AGCTTTAGGT GGAAGCTTGC TCACTTCCGT 
TACTTGTGCC AGTCTAATGC ACTACCGAGT CATGTAAAGA TCAATGTGTC CCGACAGACA 
TTATTTGAAG ATTCCTTCCA ACAGATTATG GCATTAAAAC CATATGACTT GAGGAGGCGA 
TTATATGTAA TATTTAGAGG AGAAGAAGGT CTTGATTATG GTGGCCTTGC AAGAGAATGG 
TTTTTCTTGC TTTCCCATGA AGTTTTGAAC CCAATGTATT GCTTATTTGA GTATGCAGGC 
AAGAATAACT ATTGTTTACA GATAAATCCA GCATCAACTA TAAATCCTGA CCATCTTTCA 
TACTTCTGTT TTATTGGTCG CTTTATTGCA ATGGCACTGT TCCATGGAAA GTTTATTGAT 
ACTGGTTTCT CTTTACCATT CTACAAGCGT ATGCTAAGTA AAAAACTTAC TATTAAGGAT 
TTGGAATCTA TTGATACTGA ATTTTACAAC TCTCTAATCT GGATAAGAGA TAACAATATT 
GAAGAATGTG GCTTAGAAAT GTACTTTTCT GTAGACATGG AGATTCTGGG AAAAGTTACT 
TCACATGATT TAAAGTTGGG AGGTTCCAAT ATCCTGGTGA CGGAAGAAAA CAAAGATGAA 
TATATTGGTT TAATGACAGA ATGGCGTTTT TCTCGAGGAG TACAAGAACA GACCAAAGCT 
TTCCTTGATG GGTTTAATGA AGTTGTTCCC CTTCAGTGGC TACAGTACTT TGATGAAAAA 
GAATTGGAGG TTATGCTGTG TGGCATGCAA GAGGTTGACT TGGCAGACTG GCAGAGAAAT 
ACTGTTTATC GACATTATAC AAGAAACAGC AAGCAAATTA TTTGGTTTTG GCAGTTTGTG 
AAAGAGACTG ATAATGAAGT ACGAATGCGA CTCTTGCAGT TTGTGACTGG AACCTGTCGC 
TTGCCTCTAG GAGGATTTGC TGAGCTCATG GGAAGTAATG GGCCTCAAAA GTTCTGTATT 
GAAAAAGTTG GCAAAGACAC CTGGTTACCA AGAAGCCATA CATGTTTTAA TCGCTTGGAT 
CTACCACCAT ATAAGAGTTA TGAACAACTA AAGGAAAAAC TTCTTTTTGC AATAGAAGAG 
ACAGAGGGAT TTGGACAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263221.1
CDS	515..2878
Translation	MGRAADCKCDPTDHLGVPSLESPHFKGRCSIRKSNSGLETSAVNTQQKMLPVEGTIGIDNHVSTNTVVPNSCCSHVVNGENTPSSPSQVAARPKNAPAPKPVTSAPTSDTVNGESSSVLADNTSTMGTLLPSEDTTSTSNCTSTTTQEPPVQEPPASSEHSECIPSASAEVGPEARSLIDPDSDSRNNSVFDKVRQPEGCVEPLRPQSGNTNTEALPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001276292.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276292.1

ORF Insert Sequence:

ATGGGACGAG CAGCTGATTG TAAATGTGAC CCCACAGACC ACCTTGGAGT TCCGAGTTTG 
GAGTCACCAC ACTTTAAAGG CAGATGCTCT ATTAGGAAAA GCAACAGTGG ACTTGAAACA 
AGTGCTGTTA ACACACAACA GAAAATGTTG CCTGTTGAAG GTACTATTGG AATTGATAAT 
CATGTATCAA CCAATACTGT TGTTCCCAAC TCCTGCTGTT CACATGTAGT TAATGGAGAA 
AACACACCTT CATCCCCGTC TCAGGTTGCT GCCAGACCCA AAAATGCTCC AGCTCCAAAG 
CCAGTCACAT CTGCGCCCAC CAGTGACACG GTTAATGGAG AATCATCATC TGTCCTAGCT 
GATAATACTT CAACCATGGG TACTTTACTA CCATCTGAAG ATACCACCTC GACTTCCAAT 
TGCACTAGCA CCACCACCCA AGAGCCTCCT GTACAAGAAC CCCCAGCATC CTCAGAACAC 
AGTGAATGTA TTCCCTCTGC CAGTGCGGAA GTGGGACCAG AAGCTCGGAG CCTCATAGAC 
CCTGACTCTG ATTCTAGAAA TAATTCTGTG TTTGACAAAG TAAGGCAGCC AGAAGGGTGT 
GTAGAACCTC TGCGGCCACA GTCTGGAAAC ACCAACACAG AAGCTTTGCC ATCAGGGTGG 
GAACAGAGAA AAGATCCTCA TGGTAGAACC TATTATGTGG ATCATAATAC TCGAACTACT 
ACATGGGAGA GACCACAGCC TTTGCCTCCA GGTTGGGAAA GAAGAGTTGA TGATCGTGGA 
AGAGTTTATT ATGTGGATCA TAACACCAGA ACAACAACCT GGCAGCGGCC TACTATGGAA 
TCTGTTCGAA ATTTTGAACA GTGGCAGTCT CAGCGGAATC AATTGCAGGG AGCTATGCAA 
CAATTCAACC AACGATACCT CTATTCGGCT TCCATGTTAG CTGCAGAAAA TGACCCCTAT 
GGACCCTTAC CACCGGGCTG GGAAAAAAGA GTGGATTCAA CAGACAGAGT TTACTTTGTG 
AATCACAATA CAAAAACAAC CCAGTGGGAA GATCCAAGAA CTCAAGGCTT ACCAAATGAA 
GAACCCCTGC CCGAAGGCTG GGAAATCAGG TATACTCGGG AAGGTGTTAG GTACTTTGTT 
GATCATAACA CGAGAACAAC AACATTCAAA GACCCTCGCA ATGGGAAGTC ATCAGTAACT 
AAAGGTGGTC CACAGATTGC TTATGAACGC AGCTTTAGGT GGAAGCTTGC TCACTTCCGT 
TACTTGTGCC AGTCTAATGC ACTACCGAGT CATGTAAAGA TCAATGTGTC CCGACAGACA 
TTATTTGAAG ATTCCTTCCA ACAGATTATG GCATTAAAAC CATATGACTT GAGGAGGCGA 
TTATATGTAA TATTTAGAGG AGAAGAAGGT CTTGATTATG GTGGCCTTGC AAGAGAATGG 
TTTTTCTTGC TTTCCCATGA AGTTTTGAAC CCAATGTATT GCTTATTTGA GTATGCAGGC 
AAGAATAACT ATTGTTTACA GATAAATCCA GCATCAACTA TAAATCCTGA CCATCTTTCA 
TACTTCTGTT TTATTGGTCG CTTTATTGCA ATGGCACTGT TCCATGGAAA GTTTATTGAT 
ACTGGTTTCT CTTTACCATT CTACAAGCGT ATGCTAAGTA AAAAACTTAC TATTAAGGAT 
TTGGAATCTA TTGATACTGA ATTTTACAAC TCTCTAATCT GGATAAGAGA TAACAATATT 
GAAGAATGTG GCTTAGAAAT GTACTTTTCT GTAGACATGG AGATTCTGGG AAAAGTTACT 
TCACATGATT TAAAGTTGGG AGGTTCCAAT ATCCTGGTGA CGGAAGAAAA CAAAGATGAA 
TATATTGGTT TAATGACAGA ATGGCGTTTT TCTCGAGGAG TACAAGAACA GACCAAAGCT 
TTCCTTGATG GGTTTAATGA AGTTGTTCCC CTTCAGTGGC TACAGTACTT TGATGAAAAA 
GAATTGGAGG TTATGCTGTG TGGCATGCAA GAGGTTGACT TGGCAGACTG GCAGAGAAAT 
ACTGTTTATC GACATTATAC AAGAAACAGC AAGCAAATTA TTTGGTTTTG GCAGTTTGTG 
AAAGAGACTG ATAATGAAGT ACGAATGCGA CTCTTGCAGT TTGTGACTGG AACCTGTCGC 
TTGCCTCTAG GAGGATTTGC TGAGCTCATG GGAAGTAATG GGCCTCAAAA GTTCTGTATT 
GAAAAAGTTG GCAAAGACAC CTGGTTACCA AGAAGCCATA CATGTTTTAA TCGCTTGGAT 
CTACCACCAT ATAAGAGTTA TGAACAACTA AAGGAAAAAC TTCTTTTTGC AATAGAAGAG 
ACAGAGGGAT TTGGACAAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wwp1 ( NM_177327.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796301.2 Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14063
Clone ID Related Accession (Same CDS sequence)	NM_177327.5
Accession Version	NM_177327.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ101217.1, AK137588.1, AL683894.6, AK031762.1 and AK033138.1. On Jan 26, 2013 this sequence version replaced NM_177327.4. Transcript Variant: This variant (1) represents the longr transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.237463.1, SRR1660817.169196.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTG CTTCACCAAG ATCTGACACT AGTGATATCC ACAGTGGAAG GTTACAGTTA 
AAGGTAACGG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA 
TATACTGAAG TCATTGTAGA TGGAGAAGTT AAGAAAACAG CAAAATCCAG TAGTTCCTCT 
AATCCAAAAT GGGACGAGCA GCTGATTGTA AATGTGACCC CACAGACCAC CTTGGAGTTC 
CGAGTTTGGA GTCACCACAC TTTAAAGGCA GATGCTCTAT TAGGAAAAGC AACAGTGGAC 
TTGAAACAAG TGCTGTTAAC ACACAACAGA AAATTAGAAA AAGTGAAAGA ACAATTAAAA 
CTTTCCTTGG AAAACAAGAA TGGAATAGTG CAAACTGGGG AACTGACAGT TGTCCTTGAT 
GGATTAGTGA TTGAGCAAGA GCCTGTAACA AACCGCAGCT CCTCGCCACC CATAGAAATT 
CAGCAAAATG GAGATGCCTT ACATGAGAAT GGAGACCCTG CAACAAGGAC AACTCCCAGG 
TTGCCTGTTG AAGGTACTAT TGGAATTGAT AATCATGTAT CAACCAATAC TGTTGTTCCC 
AACTCCTGCT GTTCACATGT AGTTAATGGA GAAAACACAC CTTCATCCCC GTCTCAGGTT 
GCTGCCAGAC CCAAAAATGC TCCAGCTCCA AAGCCAGTCA CATCTGCGCC CACCAGTGAC 
ACGGTTAATG GAGAATCATC ATCTGTCCTA GCTGATAATA CTTCAACCAT GGGTACTTTA 
CTACCATCTG AAGATACCAC CTCGACTTCC AATTGCACTA GCACCACCAC CCAAGAGCCT 
CCTGTACAAG AACCCCCAGC ATCCTCAGAA CACAGTGAAT GTATTCCCTC TGCCAGTGCG 
GAAGTGGGAC CAGAAGCTCG GAGCCTCATA GACCCTGACT CTGATTCTAG AAATAATTCT 
GTGTTTGACA AAGTAAGGCA GCCAGAAGGG TGTGTAGAAC CTCTGCGGCC ACAGTCTGGA 
AACACCAACA CAGAAGCTTT GCCATCAGGG TGGGAACAGA GAAAAGATCC TCATGGTAGA 
ACCTATTATG TGGATCATAA TACTCGAACT ACTACATGGG AGAGACCACA GCCTTTGCCT 
CCAGGTTGGG AAAGAAGAGT TGATGATCGT GGAAGAGTTT ATTATGTGGA TCATAACACC 
AGAACAACAA CCTGGCAGCG GCCTACTATG GAATCTGTTC GAAATTTTGA ACAGTGGCAG 
TCTCAGCGGA ATCAATTGCA GGGAGCTATG CAACAATTCA ACCAACGATA CCTCTATTCG 
GCTTCCATGT TAGCTGCAGA AAATGACCCC TATGGACCCT TACCACCGGG CTGGGAAAAA 
AGAGTGGATT CAACAGACAG AGTTTACTTT GTGAATCACA ATACAAAAAC AACCCAGTGG 
GAAGATCCAA GAACTCAAGG CTTACCAAAT GAAGAACCCC TGCCCGAAGG CTGGGAAATC 
AGGTATACTC GGGAAGGTGT TAGGTACTTT GTTGATCATA ACACGAGAAC AACAACATTC 
AAAGACCCTC GCAATGGGAA GTCATCAGTA ACTAAAGGTG GTCCACAGAT TGCTTATGAA 
CGCAGCTTTA GGTGGAAGCT TGCTCACTTC CGTTACTTGT GCCAGTCTAA TGCACTACCG 
AGTCATGTAA AGATCAATGT GTCCCGACAG ACATTATTTG AAGATTCCTT CCAACAGATT 
ATGGCATTAA AACCATATGA CTTGAGGAGG CGATTATATG TAATATTTAG AGGAGAAGAA 
GGTCTTGATT ATGGTGGCCT TGCAAGAGAA TGGTTTTTCT TGCTTTCCCA TGAAGTTTTG 
AACCCAATGT ATTGCTTATT TGAGTATGCA GGCAAGAATA ACTATTGTTT ACAGATAAAT 
CCAGCATCAA CTATAAATCC TGACCATCTT TCATACTTCT GTTTTATTGG TCGCTTTATT 
GCAATGGCAC TGTTCCATGG AAAGTTTATT GATACTGGTT TCTCTTTACC ATTCTACAAG 
CGTATGCTAA GTAAAAAACT TACTATTAAG GATTTGGAAT CTATTGATAC TGAATTTTAC 
AACTCTCTAA TCTGGATAAG AGATAACAAT ATTGAAGAAT GTGGCTTAGA AATGTACTTT 
TCTGTAGACA TGGAGATTCT GGGAAAAGTT ACTTCACATG ATTTAAAGTT GGGAGGTTCC 
AATATCCTGG TGACGGAAGA AAACAAAGAT GAATATATTG GTTTAATGAC AGAATGGCGT 
TTTTCTCGAG GAGTACAAGA ACAGACCAAA GCTTTCCTTG ATGGGTTTAA TGAAGTTGTT 
CCCCTTCAGT GGCTACAGTA CTTTGATGAA AAAGAATTGG AGGTTATGCT GTGTGGCATG 
CAAGAGGTTG ACTTGGCAGA CTGGCAGAGA AATACTGTTT ATCGACATTA TACAAGAAAC 
AGCAAGCAAA TTATTTGGTT TTGGCAGTTT GTGAAAGAGA CTGATAATGA AGTACGAATG 
CGACTCTTGC AGTTTGTGAC TGGAACCTGT CGCTTGCCTC TAGGAGGATT TGCTGAGCTC 
ATGGGAAGTA ATGGGCCTCA AAAGTTCTGT ATTGAAAAAG TTGGCAAAGA CACCTGGTTA 
CCAAGAAGCC ATACATGTTT TAATCGCTTG GATCTACCAC CATATAAGAG TTATGAACAA 
CTAAAGGAAA AACTTCTTTT TGCAATAGAA GAGACAGAGG GATTTGGACA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796301.2
CDS	284..3040
Translation	MATASPRSDTSDIHSGRLQLKVTVSSAKLKRKKNWFGTAIYTEVIVDGEVKKTAKSSSSSNPKWDEQLIVNVTPQTTLEFRVWSHHTLKADALLGKATVDLKQVLLTHNRKLEKVKEQLKLSLENKNGIVQTGELTVVLDGLVIEQEPVTNRSSSPPIEIQQNGDALHENGDPATRTTPRLPVEGTIGIDNHVSTNTVVPNSCCSHVVNGENTPSSPSQVAARPKNAPAPKPVTSAPTSDTVNGESSSVLADNTSTMGTLLPSEDTTSTSNCTSTTTQEPPVQEPPASSEHSECIPSASAEVGPEARSLIDPDSDSRNNSVFDKVRQPEGCVEPLRPQSGNTNTEALPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_177327.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177327.5

ORF Insert Sequence:

ATGGCCACTG CTTCACCAAG ATCTGACACT AGTGATATCC ACAGTGGAAG GTTACAGTTA 
AAGGTAACGG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA 
TATACTGAAG TCATTGTAGA TGGAGAAGTT AAGAAAACAG CAAAATCCAG TAGTTCCTCT 
AATCCAAAAT GGGACGAGCA GCTGATTGTA AATGTGACCC CACAGACCAC CTTGGAGTTC 
CGAGTTTGGA GTCACCACAC TTTAAAGGCA GATGCTCTAT TAGGAAAAGC AACAGTGGAC 
TTGAAACAAG TGCTGTTAAC ACACAACAGA AAATTAGAAA AAGTGAAAGA ACAATTAAAA 
CTTTCCTTGG AAAACAAGAA TGGAATAGTG CAAACTGGGG AACTGACAGT TGTCCTTGAT 
GGATTAGTGA TTGAGCAAGA GCCTGTAACA AACCGCAGCT CCTCGCCACC CATAGAAATT 
CAGCAAAATG GAGATGCCTT ACATGAGAAT GGAGACCCTG CAACAAGGAC AACTCCCAGG 
TTGCCTGTTG AAGGTACTAT TGGAATTGAT AATCATGTAT CAACCAATAC TGTTGTTCCC 
AACTCCTGCT GTTCACATGT AGTTAATGGA GAAAACACAC CTTCATCCCC GTCTCAGGTT 
GCTGCCAGAC CCAAAAATGC TCCAGCTCCA AAGCCAGTCA CATCTGCGCC CACCAGTGAC 
ACGGTTAATG GAGAATCATC ATCTGTCCTA GCTGATAATA CTTCAACCAT GGGTACTTTA 
CTACCATCTG AAGATACCAC CTCGACTTCC AATTGCACTA GCACCACCAC CCAAGAGCCT 
CCTGTACAAG AACCCCCAGC ATCCTCAGAA CACAGTGAAT GTATTCCCTC TGCCAGTGCG 
GAAGTGGGAC CAGAAGCTCG GAGCCTCATA GACCCTGACT CTGATTCTAG AAATAATTCT 
GTGTTTGACA AAGTAAGGCA GCCAGAAGGG TGTGTAGAAC CTCTGCGGCC ACAGTCTGGA 
AACACCAACA CAGAAGCTTT GCCATCAGGG TGGGAACAGA GAAAAGATCC TCATGGTAGA 
ACCTATTATG TGGATCATAA TACTCGAACT ACTACATGGG AGAGACCACA GCCTTTGCCT 
CCAGGTTGGG AAAGAAGAGT TGATGATCGT GGAAGAGTTT ATTATGTGGA TCATAACACC 
AGAACAACAA CCTGGCAGCG GCCTACTATG GAATCTGTTC GAAATTTTGA ACAGTGGCAG 
TCTCAGCGGA ATCAATTGCA GGGAGCTATG CAACAATTCA ACCAACGATA CCTCTATTCG 
GCTTCCATGT TAGCTGCAGA AAATGACCCC TATGGACCCT TACCACCGGG CTGGGAAAAA 
AGAGTGGATT CAACAGACAG AGTTTACTTT GTGAATCACA ATACAAAAAC AACCCAGTGG 
GAAGATCCAA GAACTCAAGG CTTACCAAAT GAAGAACCCC TGCCCGAAGG CTGGGAAATC 
AGGTATACTC GGGAAGGTGT TAGGTACTTT GTTGATCATA ACACGAGAAC AACAACATTC 
AAAGACCCTC GCAATGGGAA GTCATCAGTA ACTAAAGGTG GTCCACAGAT TGCTTATGAA 
CGCAGCTTTA GGTGGAAGCT TGCTCACTTC CGTTACTTGT GCCAGTCTAA TGCACTACCG 
AGTCATGTAA AGATCAATGT GTCCCGACAG ACATTATTTG AAGATTCCTT CCAACAGATT 
ATGGCATTAA AACCATATGA CTTGAGGAGG CGATTATATG TAATATTTAG AGGAGAAGAA 
GGTCTTGATT ATGGTGGCCT TGCAAGAGAA TGGTTTTTCT TGCTTTCCCA TGAAGTTTTG 
AACCCAATGT ATTGCTTATT TGAGTATGCA GGCAAGAATA ACTATTGTTT ACAGATAAAT 
CCAGCATCAA CTATAAATCC TGACCATCTT TCATACTTCT GTTTTATTGG TCGCTTTATT 
GCAATGGCAC TGTTCCATGG AAAGTTTATT GATACTGGTT TCTCTTTACC ATTCTACAAG 
CGTATGCTAA GTAAAAAACT TACTATTAAG GATTTGGAAT CTATTGATAC TGAATTTTAC 
AACTCTCTAA TCTGGATAAG AGATAACAAT ATTGAAGAAT GTGGCTTAGA AATGTACTTT 
TCTGTAGACA TGGAGATTCT GGGAAAAGTT ACTTCACATG ATTTAAAGTT GGGAGGTTCC 
AATATCCTGG TGACGGAAGA AAACAAAGAT GAATATATTG GTTTAATGAC AGAATGGCGT 
TTTTCTCGAG GAGTACAAGA ACAGACCAAA GCTTTCCTTG ATGGGTTTAA TGAAGTTGTT 
CCCCTTCAGT GGCTACAGTA CTTTGATGAA AAAGAATTGG AGGTTATGCT GTGTGGCATG 
CAAGAGGTTG ACTTGGCAGA CTGGCAGAGA AATACTGTTT ATCGACATTA TACAAGAAAC 
AGCAAGCAAA TTATTTGGTT TTGGCAGTTT GTGAAAGAGA CTGATAATGA AGTACGAATG 
CGACTCTTGC AGTTTGTGAC TGGAACCTGT CGCTTGCCTC TAGGAGGATT TGCTGAGCTC 
ATGGGAAGTA ATGGGCCTCA AAAGTTCTGT ATTGAAAAAG TTGGCAAAGA CACCTGGTTA 
CCAAGAAGCC ATACATGTTT TAATCGCTTG GATCTACCAC CATATAAGAG TTATGAACAA 
CTAAAGGAAA AACTTCTTTT TGCAATAGAA GAGACAGAGG GATTTGGACA AGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wwp1 ( NM_001355222.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342151.1 Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu32681
Clone ID Related Accession (Same CDS sequence)	NM_001355222.1 , XM_006537545.3
Accession Version	NM_001355222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ101217.1, BY739197.1, AK168567.1, AK032232.1, AL683894.6, AK031762.1 and AK033138.1. On Sep 20, 2017 this sequence version replaced XM_006537545.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.26543.1, SRR1660821.100882.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGACGAG CAGCTGATTG TACCACCTTG GAGTTCCGAG TTTGGAGTCA CCACACTTTA 
AAGGCAGATG CTCTATTAGG AAAAGCAACA GTGGACTTGA AACAAGTGCT GTTAACACAC 
AACAGAAAAT TAGAAAAAGT GAAAGAACAA TTAAAACTTT CCTTGGAAAA CAAGAATGGA 
ATAGTGCAAA CTGGGGAACT GACAGTTGTC CTTGATGGAT TAGTGATTGA GCAAGAGCCT 
GTAACAAACC GCAGCTCCTC GCCACCCATA GAAATTCAGC AAAATGGAGA TGCCTTACAT 
GAGAATGGAG ACCCTGCAAC AAGGACAACT CCCAGGTTGC CTGTTGAAGG TACTATTGGA 
ATTGATAATC ATGTATCAAC CAATACTGTT GTTCCCAACT CCTGCTGTTC ACATGTAGTT 
AATGGAGAAA ACACACCTTC ATCCCCGTCT CAGGTTGCTG CCAGACCCAA AAATGCTCCA 
GCTCCAAAGC CAGTCACATC TGCGCCCACC AGTGACACGG TTAATGGAGA ATCATCATCT 
GTCCTAGCTG ATAATACTTC AACCATGGGT ACTTTACTAC CATCTGAAGA TACCACCTCG 
ACTTCCAATT GCACTAGCAC CACCACCCAA GAGCCTCCTG TACAAGAACC CCCAGCATCC 
TCAGAACACA GTGAATGTAT TCCCTCTGCC AGTGCGGAAG TGGGACCAGA AGCTCGGAGC 
CTCATAGACC CTGACTCTGA TTCTAGAAAT AATTCTGTGT TTGACAAAGT AAGGCAGCCA 
GAAGGGTGTG TAGAACCTCT GCGGCCACAG TCTGGAAACA CCAACACAGA AGCTTTGCCA 
TCAGGGTGGG AACAGAGAAA AGATCCTCAT GGTAGAACCT ATTATGTGGA TCATAATACT 
CGAACTACTA CATGGGAGAG ACCACAGCCT TTGCCTCCAG GTTGGGAAAG AAGAGTTGAT 
GATCGTGGAA GAGTTTATTA TGTGGATCAT AACACCAGAA CAACAACCTG GCAGCGGCCT 
ACTATGGAAT CTGTTCGAAA TTTTGAACAG TGGCAGTCTC AGCGGAATCA ATTGCAGGGA 
GCTATGCAAC AATTCAACCA ACGATACCTC TATTCGGCTT CCATGTTAGC TGCAGAAAAT 
GACCCCTATG GACCCTTACC ACCGGGCTGG GAAAAAAGAG TGGATTCAAC AGACAGAGTT 
TACTTTGTGA ATCACAATAC AAAAACAACC CAGTGGGAAG ATCCAAGAAC TCAAGGCTTA 
CCAAATGAAG AACCCCTGCC CGAAGGCTGG GAAATCAGGT ATACTCGGGA AGGTGTTAGG 
TACTTTGTTG ATCATAACAC GAGAACAACA ACATTCAAAG ACCCTCGCAA TGGGAAGTCA 
TCAGTAACTA AAGGTGGTCC ACAGATTGCT TATGAACGCA GCTTTAGGTG GAAGCTTGCT 
CACTTCCGTT ACTTGTGCCA GTCTAATGCA CTACCGAGTC ATGTAAAGAT CAATGTGTCC 
CGACAGACAT TATTTGAAGA TTCCTTCCAA CAGATTATGG CATTAAAACC ATATGACTTG 
AGGAGGCGAT TATATGTAAT ATTTAGAGGA GAAGAAGGTC TTGATTATGG TGGCCTTGCA 
AGAGAATGGT TTTTCTTGCT TTCCCATGAA GTTTTGAACC CAATGTATTG CTTATTTGAG 
TATGCAGGCA AGAATAACTA TTGTTTACAG ATAAATCCAG CATCAACTAT AAATCCTGAC 
CATCTTTCAT ACTTCTGTTT TATTGGTCGC TTTATTGCAA TGGCACTGTT CCATGGAAAG 
TTTATTGATA CTGGTTTCTC TTTACCATTC TACAAGCGTA TGCTAAGTAA AAAACTTACT 
ATTAAGGATT TGGAATCTAT TGATACTGAA TTTTACAACT CTCTAATCTG GATAAGAGAT 
AACAATATTG AAGAATGTGG CTTAGAAATG TACTTTTCTG TAGACATGGA GATTCTGGGA 
AAAGTTACTT CACATGATTT AAAGTTGGGA GGTTCCAATA TCCTGGTGAC GGAAGAAAAC 
AAAGATGAAT ATATTGGTTT AATGACAGAA TGGCGTTTTT CTCGAGGAGT ACAAGAACAG 
ACCAAAGCTT TCCTTGATGG GTTTAATGAA GTTGTTCCCC TTCAGTGGCT ACAGTACTTT 
GATGAAAAAG AATTGGAGGT TATGCTGTGT GGCATGCAAG AGGTTGACTT GGCAGACTGG 
CAGAGAAATA CTGTTTATCG ACATTATACA AGAAACAGCA AGCAAATTAT TTGGTTTTGG 
CAGTTTGTGA AAGAGACTGA TAATGAAGTA CGAATGCGAC TCTTGCAGTT TGTGACTGGA 
ACCTGTCGCT TGCCTCTAGG AGGATTTGCT GAGCTCATGG GAAGTAATGG GCCTCAAAAG 
TTCTGTATTG AAAAAGTTGG CAAAGACACC TGGTTACCAA GAAGCCATAC ATGTTTTAAT 
CGCTTGGATC TACCACCATA TAAGAGTTAT GAACAACTAA AGGAAAAACT TCTTTTTGCA 
ATAGAAGAGA CAGAGGGATT TGGACAAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342151.1
CDS	472..3024
Translation	MGRAADCTTLEFRVWSHHTLKADALLGKATVDLKQVLLTHNRKLEKVKEQLKLSLENKNGIVQTGELTVVLDGLVIEQEPVTNRSSSPPIEIQQNGDALHENGDPATRTTPRLPVEGTIGIDNHVSTNTVVPNSCCSHVVNGENTPSSPSQVAARPKNAPAPKPVTSAPTSDTVNGESSSVLADNTSTMGTLLPSEDTTSTSNCTSTTTQEPPVQEPPASSEHSECIPSASAEVGPEARSLIDPDSDSRNNSVFDKVRQPEGCVEPLRPQSGNTNTEALPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001355222.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355222.1

ORF Insert Sequence:

ATGGGACGAG CAGCTGATTG TACCACCTTG GAGTTCCGAG TTTGGAGTCA CCACACTTTA 
AAGGCAGATG CTCTATTAGG AAAAGCAACA GTGGACTTGA AACAAGTGCT GTTAACACAC 
AACAGAAAAT TAGAAAAAGT GAAAGAACAA TTAAAACTTT CCTTGGAAAA CAAGAATGGA 
ATAGTGCAAA CTGGGGAACT GACAGTTGTC CTTGATGGAT TAGTGATTGA GCAAGAGCCT 
GTAACAAACC GCAGCTCCTC GCCACCCATA GAAATTCAGC AAAATGGAGA TGCCTTACAT 
GAGAATGGAG ACCCTGCAAC AAGGACAACT CCCAGGTTGC CTGTTGAAGG TACTATTGGA 
ATTGATAATC ATGTATCAAC CAATACTGTT GTTCCCAACT CCTGCTGTTC ACATGTAGTT 
AATGGAGAAA ACACACCTTC ATCCCCGTCT CAGGTTGCTG CCAGACCCAA AAATGCTCCA 
GCTCCAAAGC CAGTCACATC TGCGCCCACC AGTGACACGG TTAATGGAGA ATCATCATCT 
GTCCTAGCTG ATAATACTTC AACCATGGGT ACTTTACTAC CATCTGAAGA TACCACCTCG 
ACTTCCAATT GCACTAGCAC CACCACCCAA GAGCCTCCTG TACAAGAACC CCCAGCATCC 
TCAGAACACA GTGAATGTAT TCCCTCTGCC AGTGCGGAAG TGGGACCAGA AGCTCGGAGC 
CTCATAGACC CTGACTCTGA TTCTAGAAAT AATTCTGTGT TTGACAAAGT AAGGCAGCCA 
GAAGGGTGTG TAGAACCTCT GCGGCCACAG TCTGGAAACA CCAACACAGA AGCTTTGCCA 
TCAGGGTGGG AACAGAGAAA AGATCCTCAT GGTAGAACCT ATTATGTGGA TCATAATACT 
CGAACTACTA CATGGGAGAG ACCACAGCCT TTGCCTCCAG GTTGGGAAAG AAGAGTTGAT 
GATCGTGGAA GAGTTTATTA TGTGGATCAT AACACCAGAA CAACAACCTG GCAGCGGCCT 
ACTATGGAAT CTGTTCGAAA TTTTGAACAG TGGCAGTCTC AGCGGAATCA ATTGCAGGGA 
GCTATGCAAC AATTCAACCA ACGATACCTC TATTCGGCTT CCATGTTAGC TGCAGAAAAT 
GACCCCTATG GACCCTTACC ACCGGGCTGG GAAAAAAGAG TGGATTCAAC AGACAGAGTT 
TACTTTGTGA ATCACAATAC AAAAACAACC CAGTGGGAAG ATCCAAGAAC TCAAGGCTTA 
CCAAATGAAG AACCCCTGCC CGAAGGCTGG GAAATCAGGT ATACTCGGGA AGGTGTTAGG 
TACTTTGTTG ATCATAACAC GAGAACAACA ACATTCAAAG ACCCTCGCAA TGGGAAGTCA 
TCAGTAACTA AAGGTGGTCC ACAGATTGCT TATGAACGCA GCTTTAGGTG GAAGCTTGCT 
CACTTCCGTT ACTTGTGCCA GTCTAATGCA CTACCGAGTC ATGTAAAGAT CAATGTGTCC 
CGACAGACAT TATTTGAAGA TTCCTTCCAA CAGATTATGG CATTAAAACC ATATGACTTG 
AGGAGGCGAT TATATGTAAT ATTTAGAGGA GAAGAAGGTC TTGATTATGG TGGCCTTGCA 
AGAGAATGGT TTTTCTTGCT TTCCCATGAA GTTTTGAACC CAATGTATTG CTTATTTGAG 
TATGCAGGCA AGAATAACTA TTGTTTACAG ATAAATCCAG CATCAACTAT AAATCCTGAC 
CATCTTTCAT ACTTCTGTTT TATTGGTCGC TTTATTGCAA TGGCACTGTT CCATGGAAAG 
TTTATTGATA CTGGTTTCTC TTTACCATTC TACAAGCGTA TGCTAAGTAA AAAACTTACT 
ATTAAGGATT TGGAATCTAT TGATACTGAA TTTTACAACT CTCTAATCTG GATAAGAGAT 
AACAATATTG AAGAATGTGG CTTAGAAATG TACTTTTCTG TAGACATGGA GATTCTGGGA 
AAAGTTACTT CACATGATTT AAAGTTGGGA GGTTCCAATA TCCTGGTGAC GGAAGAAAAC 
AAAGATGAAT ATATTGGTTT AATGACAGAA TGGCGTTTTT CTCGAGGAGT ACAAGAACAG 
ACCAAAGCTT TCCTTGATGG GTTTAATGAA GTTGTTCCCC TTCAGTGGCT ACAGTACTTT 
GATGAAAAAG AATTGGAGGT TATGCTGTGT GGCATGCAAG AGGTTGACTT GGCAGACTGG 
CAGAGAAATA CTGTTTATCG ACATTATACA AGAAACAGCA AGCAAATTAT TTGGTTTTGG 
CAGTTTGTGA AAGAGACTGA TAATGAAGTA CGAATGCGAC TCTTGCAGTT TGTGACTGGA 
ACCTGTCGCT TGCCTCTAGG AGGATTTGCT GAGCTCATGG GAAGTAATGG GCCTCAAAAG 
TTCTGTATTG AAAAAGTTGG CAAAGACACC TGGTTACCAA GAAGCCATAC ATGTTTTAAT 
CGCTTGGATC TACCACCATA TAAGAGTTAT GAACAACTAA AGGAAAAACT TCTTTTTGCA 
ATAGAAGAGA CAGAGGGATT TGGACAAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wwp1 ( XM_006537545.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537608.1 Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu32681
Clone ID Related Accession (Same CDS sequence)	NM_001355222.1 , XM_006537545.3
Accession Version	XM_006537545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537545.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACGAG CAGCTGATTG TACCACCTTG GAGTTCCGAG TTTGGAGTCA CCACACTTTA 
AAGGCAGATG CTCTATTAGG AAAAGCAACA GTGGACTTGA AACAAGTGCT GTTAACACAC 
AACAGAAAAT TAGAAAAAGT GAAAGAACAA TTAAAACTTT CCTTGGAAAA CAAGAATGGA 
ATAGTGCAAA CTGGGGAACT GACAGTTGTC CTTGATGGAT TAGTGATTGA GCAAGAGCCT 
GTAACAAACC GCAGCTCCTC GCCACCCATA GAAATTCAGC AAAATGGAGA TGCCTTACAT 
GAGAATGGAG ACCCTGCAAC AAGGACAACT CCCAGGTTGC CTGTTGAAGG TACTATTGGA 
ATTGATAATC ATGTATCAAC CAATACTGTT GTTCCCAACT CCTGCTGTTC ACATGTAGTT 
AATGGAGAAA ACACACCTTC ATCCCCGTCT CAGGTTGCTG CCAGACCCAA AAATGCTCCA 
GCTCCAAAGC CAGTCACATC TGCGCCCACC AGTGACACGG TTAATGGAGA ATCATCATCT 
GTCCTAGCTG ATAATACTTC AACCATGGGT ACTTTACTAC CATCTGAAGA TACCACCTCG 
ACTTCCAATT GCACTAGCAC CACCACCCAA GAGCCTCCTG TACAAGAACC CCCAGCATCC 
TCAGAACACA GTGAATGTAT TCCCTCTGCC AGTGCGGAAG TGGGACCAGA AGCTCGGAGC 
CTCATAGACC CTGACTCTGA TTCTAGAAAT AATTCTGTGT TTGACAAAGT AAGGCAGCCA 
GAAGGGTGTG TAGAACCTCT GCGGCCACAG TCTGGAAACA CCAACACAGA AGCTTTGCCA 
TCAGGGTGGG AACAGAGAAA AGATCCTCAT GGTAGAACCT ATTATGTGGA TCATAATACT 
CGAACTACTA CATGGGAGAG ACCACAGCCT TTGCCTCCAG GTTGGGAAAG AAGAGTTGAT 
GATCGTGGAA GAGTTTATTA TGTGGATCAT AACACCAGAA CAACAACCTG GCAGCGGCCT 
ACTATGGAAT CTGTTCGAAA TTTTGAACAG TGGCAGTCTC AGCGGAATCA ATTGCAGGGA 
GCTATGCAAC AATTCAACCA ACGATACCTC TATTCGGCTT CCATGTTAGC TGCAGAAAAT 
GACCCCTATG GACCCTTACC ACCGGGCTGG GAAAAAAGAG TGGATTCAAC AGACAGAGTT 
TACTTTGTGA ATCACAATAC AAAAACAACC CAGTGGGAAG ATCCAAGAAC TCAAGGCTTA 
CCAAATGAAG AACCCCTGCC CGAAGGCTGG GAAATCAGGT ATACTCGGGA AGGTGTTAGG 
TACTTTGTTG ATCATAACAC GAGAACAACA ACATTCAAAG ACCCTCGCAA TGGGAAGTCA 
TCAGTAACTA AAGGTGGTCC ACAGATTGCT TATGAACGCA GCTTTAGGTG GAAGCTTGCT 
CACTTCCGTT ACTTGTGCCA GTCTAATGCA CTACCGAGTC ATGTAAAGAT CAATGTGTCC 
CGACAGACAT TATTTGAAGA TTCCTTCCAA CAGATTATGG CATTAAAACC ATATGACTTG 
AGGAGGCGAT TATATGTAAT ATTTAGAGGA GAAGAAGGTC TTGATTATGG TGGCCTTGCA 
AGAGAATGGT TTTTCTTGCT TTCCCATGAA GTTTTGAACC CAATGTATTG CTTATTTGAG 
TATGCAGGCA AGAATAACTA TTGTTTACAG ATAAATCCAG CATCAACTAT AAATCCTGAC 
CATCTTTCAT ACTTCTGTTT TATTGGTCGC TTTATTGCAA TGGCACTGTT CCATGGAAAG 
TTTATTGATA CTGGTTTCTC TTTACCATTC TACAAGCGTA TGCTAAGTAA AAAACTTACT 
ATTAAGGATT TGGAATCTAT TGATACTGAA TTTTACAACT CTCTAATCTG GATAAGAGAT 
AACAATATTG AAGAATGTGG CTTAGAAATG TACTTTTCTG TAGACATGGA GATTCTGGGA 
AAAGTTACTT CACATGATTT AAAGTTGGGA GGTTCCAATA TCCTGGTGAC GGAAGAAAAC 
AAAGATGAAT ATATTGGTTT AATGACAGAA TGGCGTTTTT CTCGAGGAGT ACAAGAACAG 
ACCAAAGCTT TCCTTGATGG GTTTAATGAA GTTGTTCCCC TTCAGTGGCT ACAGTACTTT 
GATGAAAAAG AATTGGAGGT TATGCTGTGT GGCATGCAAG AGGTTGACTT GGCAGACTGG 
CAGAGAAATA CTGTTTATCG ACATTATACA AGAAACAGCA AGCAAATTAT TTGGTTTTGG 
CAGTTTGTGA AAGAGACTGA TAATGAAGTA CGAATGCGAC TCTTGCAGTT TGTGACTGGA 
ACCTGTCGCT TGCCTCTAGG AGGATTTGCT GAGCTCATGG GAAGTAATGG GCCTCAAAAG 
TTCTGTATTG AAAAAGTTGG CAAAGACACC TGGTTACCAA GAAGCCATAC ATGTTTTAAT 
CGCTTGGATC TACCACCATA TAAGAGTTAT GAACAACTAA AGGAAAAACT TCTTTTTGCA 
ATAGAAGAGA CAGAGGGATT TGGACAAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537608.1
CDS	474..3026
Translation	MGRAADCTTLEFRVWSHHTLKADALLGKATVDLKQVLLTHNRKLEKVKEQLKLSLENKNGIVQTGELTVVLDGLVIEQEPVTNRSSSPPIEIQQNGDALHENGDPATRTTPRLPVEGTIGIDNHVSTNTVVPNSCCSHVVNGENTPSSPSQVAARPKNAPAPKPVTSAPTSDTVNGESSSVLADNTSTMGTLLPSEDTTSTSNCTSTTTQEPPVQEPPASSEHSECIPSASAEVGPEARSLIDPDSDSRNNSVFDKVRQPEGCVEPLRPQSGNTNTEALPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_006537545.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537545.3

ORF Insert Sequence:

ATGGGACGAG CAGCTGATTG TACCACCTTG GAGTTCCGAG TTTGGAGTCA CCACACTTTA 
AAGGCAGATG CTCTATTAGG AAAAGCAACA GTGGACTTGA AACAAGTGCT GTTAACACAC 
AACAGAAAAT TAGAAAAAGT GAAAGAACAA TTAAAACTTT CCTTGGAAAA CAAGAATGGA 
ATAGTGCAAA CTGGGGAACT GACAGTTGTC CTTGATGGAT TAGTGATTGA GCAAGAGCCT 
GTAACAAACC GCAGCTCCTC GCCACCCATA GAAATTCAGC AAAATGGAGA TGCCTTACAT 
GAGAATGGAG ACCCTGCAAC AAGGACAACT CCCAGGTTGC CTGTTGAAGG TACTATTGGA 
ATTGATAATC ATGTATCAAC CAATACTGTT GTTCCCAACT CCTGCTGTTC ACATGTAGTT 
AATGGAGAAA ACACACCTTC ATCCCCGTCT CAGGTTGCTG CCAGACCCAA AAATGCTCCA 
GCTCCAAAGC CAGTCACATC TGCGCCCACC AGTGACACGG TTAATGGAGA ATCATCATCT 
GTCCTAGCTG ATAATACTTC AACCATGGGT ACTTTACTAC CATCTGAAGA TACCACCTCG 
ACTTCCAATT GCACTAGCAC CACCACCCAA GAGCCTCCTG TACAAGAACC CCCAGCATCC 
TCAGAACACA GTGAATGTAT TCCCTCTGCC AGTGCGGAAG TGGGACCAGA AGCTCGGAGC 
CTCATAGACC CTGACTCTGA TTCTAGAAAT AATTCTGTGT TTGACAAAGT AAGGCAGCCA 
GAAGGGTGTG TAGAACCTCT GCGGCCACAG TCTGGAAACA CCAACACAGA AGCTTTGCCA 
TCAGGGTGGG AACAGAGAAA AGATCCTCAT GGTAGAACCT ATTATGTGGA TCATAATACT 
CGAACTACTA CATGGGAGAG ACCACAGCCT TTGCCTCCAG GTTGGGAAAG AAGAGTTGAT 
GATCGTGGAA GAGTTTATTA TGTGGATCAT AACACCAGAA CAACAACCTG GCAGCGGCCT 
ACTATGGAAT CTGTTCGAAA TTTTGAACAG TGGCAGTCTC AGCGGAATCA ATTGCAGGGA 
GCTATGCAAC AATTCAACCA ACGATACCTC TATTCGGCTT CCATGTTAGC TGCAGAAAAT 
GACCCCTATG GACCCTTACC ACCGGGCTGG GAAAAAAGAG TGGATTCAAC AGACAGAGTT 
TACTTTGTGA ATCACAATAC AAAAACAACC CAGTGGGAAG ATCCAAGAAC TCAAGGCTTA 
CCAAATGAAG AACCCCTGCC CGAAGGCTGG GAAATCAGGT ATACTCGGGA AGGTGTTAGG 
TACTTTGTTG ATCATAACAC GAGAACAACA ACATTCAAAG ACCCTCGCAA TGGGAAGTCA 
TCAGTAACTA AAGGTGGTCC ACAGATTGCT TATGAACGCA GCTTTAGGTG GAAGCTTGCT 
CACTTCCGTT ACTTGTGCCA GTCTAATGCA CTACCGAGTC ATGTAAAGAT CAATGTGTCC 
CGACAGACAT TATTTGAAGA TTCCTTCCAA CAGATTATGG CATTAAAACC ATATGACTTG 
AGGAGGCGAT TATATGTAAT ATTTAGAGGA GAAGAAGGTC TTGATTATGG TGGCCTTGCA 
AGAGAATGGT TTTTCTTGCT TTCCCATGAA GTTTTGAACC CAATGTATTG CTTATTTGAG 
TATGCAGGCA AGAATAACTA TTGTTTACAG ATAAATCCAG CATCAACTAT AAATCCTGAC 
CATCTTTCAT ACTTCTGTTT TATTGGTCGC TTTATTGCAA TGGCACTGTT CCATGGAAAG 
TTTATTGATA CTGGTTTCTC TTTACCATTC TACAAGCGTA TGCTAAGTAA AAAACTTACT 
ATTAAGGATT TGGAATCTAT TGATACTGAA TTTTACAACT CTCTAATCTG GATAAGAGAT 
AACAATATTG AAGAATGTGG CTTAGAAATG TACTTTTCTG TAGACATGGA GATTCTGGGA 
AAAGTTACTT CACATGATTT AAAGTTGGGA GGTTCCAATA TCCTGGTGAC GGAAGAAAAC 
AAAGATGAAT ATATTGGTTT AATGACAGAA TGGCGTTTTT CTCGAGGAGT ACAAGAACAG 
ACCAAAGCTT TCCTTGATGG GTTTAATGAA GTTGTTCCCC TTCAGTGGCT ACAGTACTTT 
GATGAAAAAG AATTGGAGGT TATGCTGTGT GGCATGCAAG AGGTTGACTT GGCAGACTGG 
CAGAGAAATA CTGTTTATCG ACATTATACA AGAAACAGCA AGCAAATTAT TTGGTTTTGG 
CAGTTTGTGA AAGAGACTGA TAATGAAGTA CGAATGCGAC TCTTGCAGTT TGTGACTGGA 
ACCTGTCGCT TGCCTCTAGG AGGATTTGCT GAGCTCATGG GAAGTAATGG GCCTCAAAAG 
TTCTGTATTG AAAAAGTTGG CAAAGACACC TGGTTACCAA GAAGCCATAC ATGTTTTAAT 
CGCTTGGATC TACCACCATA TAAGAGTTAT GAACAACTAA AGGAAAAACT TCTTTTTGCA 
ATAGAAGAGA CAGAGGGATT TGGACAAGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wwp1 ( XM_006537546.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537609.1 Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu32682
Clone ID Related Accession (Same CDS sequence)	XM_006537546.3 , XM_006537546.4
Accession Version	XM_006537546.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537546.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTACTT TACTACCATC TGAAGATACC ACCTCGACTT CCAATTGCAC TAGCACCACC 
ACCCAAGAGC CTCCTGTACA AGAACCCCCA GCATCCTCAG AACACAGTGA ATGTATTCCC 
TCTGCCAGTG CGGAAGTGGG ACCAGAAGCT CGGAGCCTCA TAGACCCTGA CTCTGATTCT 
AGAAATAATT CTGTGTTTGA CAAAGTAAGG CAGCCAGAAG GGTGTGTAGA ACCTCTGCGG 
CCACAGTCTG GAAACACCAA CACAGAAGCT TTGCCATCAG GGTGGGAACA GAGAAAAGAT 
CCTCATGGTA GAACCTATTA TGTGGATCAT AATACTCGAA CTACTACATG GGAGAGACCA 
CAGCCTTTGC CTCCAGGTTG GGAAAGAAGA GTTGATGATC GTGGAAGAGT TTATTATGTG 
GATCATAACA CCAGAACAAC AACCTGGCAG CGGCCTACTA TGGAATCTGT TCGAAATTTT 
GAACAGTGGC AGTCTCAGCG GAATCAATTG CAGGGAGCTA TGCAACAATT CAACCAACGA 
TACCTCTATT CGGCTTCCAT GTTAGCTGCA GAAAATGACC CCTATGGACC CTTACCACCG 
GGCTGGGAAA AAAGAGTGGA TTCAACAGAC AGAGTTTACT TTGTGAATCA CAATACAAAA 
ACAACCCAGT GGGAAGATCC AAGAACTCAA GGCTTACCAA ATGAAGAACC CCTGCCCGAA 
GGCTGGGAAA TCAGGTATAC TCGGGAAGGT GTTAGGTACT TTGTTGATCA TAACACGAGA 
ACAACAACAT TCAAAGACCC TCGCAATGGG AAGTCATCAG TAACTAAAGG TGGTCCACAG 
ATTGCTTATG AACGCAGCTT TAGGTGGAAG CTTGCTCACT TCCGTTACTT GTGCCAGTCT 
AATGCACTAC CGAGTCATGT AAAGATCAAT GTGTCCCGAC AGACATTATT TGAAGATTCC 
TTCCAACAGA TTATGGCATT AAAACCATAT GACTTGAGGA GGCGATTATA TGTAATATTT 
AGAGGAGAAG AAGGTCTTGA TTATGGTGGC CTTGCAAGAG AATGGTTTTT CTTGCTTTCC 
CATGAAGTTT TGAACCCAAT GTATTGCTTA TTTGAGTATG CAGGCAAGAA TAACTATTGT 
TTACAGATAA ATCCAGCATC AACTATAAAT CCTGACCATC TTTCATACTT CTGTTTTATT 
GGTCGCTTTA TTGCAATGGC ACTGTTCCAT GGAAAGTTTA TTGATACTGG TTTCTCTTTA 
CCATTCTACA AGCGTATGCT AAGTAAAAAA CTTACTATTA AGGATTTGGA ATCTATTGAT 
ACTGAATTTT ACAACTCTCT AATCTGGATA AGAGATAACA ATATTGAAGA ATGTGGCTTA 
GAAATGTACT TTTCTGTAGA CATGGAGATT CTGGGAAAAG TTACTTCACA TGATTTAAAG 
TTGGGAGGTT CCAATATCCT GGTGACGGAA GAAAACAAAG ATGAATATAT TGGTTTAATG 
ACAGAATGGC GTTTTTCTCG AGGAGTACAA GAACAGACCA AAGCTTTCCT TGATGGGTTT 
AATGAAGTTG TTCCCCTTCA GTGGCTACAG TACTTTGATG AAAAAGAATT GGAGGTTATG 
CTGTGTGGCA TGCAAGAGGT TGACTTGGCA GACTGGCAGA GAAATACTGT TTATCGACAT 
TATACAAGAA ACAGCAAGCA AATTATTTGG TTTTGGCAGT TTGTGAAAGA GACTGATAAT 
GAAGTACGAA TGCGACTCTT GCAGTTTGTG ACTGGAACCT GTCGCTTGCC TCTAGGAGGA 
TTTGCTGAGC TCATGGGAAG TAATGGGCCT CAAAAGTTCT GTATTGAAAA AGTTGGCAAA 
GACACCTGGT TACCAAGAAG CCATACATGT TTTAATCGCT TGGATCTACC ACCATATAAG 
AGTTATGAAC AACTAAAGGA AAAACTTCTT TTTGCAATAG AAGAGACAGA GGGATTTGGA 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537609.1
CDS	793..2781
Translation	MGTLLPSEDTTSTSNCTSTTTQEPPVQEPPASSEHSECIPSASAEVGPEARSLIDPDSDSRNNSVFDKVRQPEGCVEPLRPQSGNTNTEALPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_006537546.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537546.4

ORF Insert Sequence:

ATGGGTACTT TACTACCATC TGAAGATACC ACCTCGACTT CCAATTGCAC TAGCACCACC 
ACCCAAGAGC CTCCTGTACA AGAACCCCCA GCATCCTCAG AACACAGTGA ATGTATTCCC 
TCTGCCAGTG CGGAAGTGGG ACCAGAAGCT CGGAGCCTCA TAGACCCTGA CTCTGATTCT 
AGAAATAATT CTGTGTTTGA CAAAGTAAGG CAGCCAGAAG GGTGTGTAGA ACCTCTGCGG 
CCACAGTCTG GAAACACCAA CACAGAAGCT TTGCCATCAG GGTGGGAACA GAGAAAAGAT 
CCTCATGGTA GAACCTATTA TGTGGATCAT AATACTCGAA CTACTACATG GGAGAGACCA 
CAGCCTTTGC CTCCAGGTTG GGAAAGAAGA GTTGATGATC GTGGAAGAGT TTATTATGTG 
GATCATAACA CCAGAACAAC AACCTGGCAG CGGCCTACTA TGGAATCTGT TCGAAATTTT 
GAACAGTGGC AGTCTCAGCG GAATCAATTG CAGGGAGCTA TGCAACAATT CAACCAACGA 
TACCTCTATT CGGCTTCCAT GTTAGCTGCA GAAAATGACC CCTATGGACC CTTACCACCG 
GGCTGGGAAA AAAGAGTGGA TTCAACAGAC AGAGTTTACT TTGTGAATCA CAATACAAAA 
ACAACCCAGT GGGAAGATCC AAGAACTCAA GGCTTACCAA ATGAAGAACC CCTGCCCGAA 
GGCTGGGAAA TCAGGTATAC TCGGGAAGGT GTTAGGTACT TTGTTGATCA TAACACGAGA 
ACAACAACAT TCAAAGACCC TCGCAATGGG AAGTCATCAG TAACTAAAGG TGGTCCACAG 
ATTGCTTATG AACGCAGCTT TAGGTGGAAG CTTGCTCACT TCCGTTACTT GTGCCAGTCT 
AATGCACTAC CGAGTCATGT AAAGATCAAT GTGTCCCGAC AGACATTATT TGAAGATTCC 
TTCCAACAGA TTATGGCATT AAAACCATAT GACTTGAGGA GGCGATTATA TGTAATATTT 
AGAGGAGAAG AAGGTCTTGA TTATGGTGGC CTTGCAAGAG AATGGTTTTT CTTGCTTTCC 
CATGAAGTTT TGAACCCAAT GTATTGCTTA TTTGAGTATG CAGGCAAGAA TAACTATTGT 
TTACAGATAA ATCCAGCATC AACTATAAAT CCTGACCATC TTTCATACTT CTGTTTTATT 
GGTCGCTTTA TTGCAATGGC ACTGTTCCAT GGAAAGTTTA TTGATACTGG TTTCTCTTTA 
CCATTCTACA AGCGTATGCT AAGTAAAAAA CTTACTATTA AGGATTTGGA ATCTATTGAT 
ACTGAATTTT ACAACTCTCT AATCTGGATA AGAGATAACA ATATTGAAGA ATGTGGCTTA 
GAAATGTACT TTTCTGTAGA CATGGAGATT CTGGGAAAAG TTACTTCACA TGATTTAAAG 
TTGGGAGGTT CCAATATCCT GGTGACGGAA GAAAACAAAG ATGAATATAT TGGTTTAATG 
ACAGAATGGC GTTTTTCTCG AGGAGTACAA GAACAGACCA AAGCTTTCCT TGATGGGTTT 
AATGAAGTTG TTCCCCTTCA GTGGCTACAG TACTTTGATG AAAAAGAATT GGAGGTTATG 
CTGTGTGGCA TGCAAGAGGT TGACTTGGCA GACTGGCAGA GAAATACTGT TTATCGACAT 
TATACAAGAA ACAGCAAGCA AATTATTTGG TTTTGGCAGT TTGTGAAAGA GACTGATAAT 
GAAGTACGAA TGCGACTCTT GCAGTTTGTG ACTGGAACCT GTCGCTTGCC TCTAGGAGGA 
TTTGCTGAGC TCATGGGAAG TAATGGGCCT CAAAAGTTCT GTATTGAAAA AGTTGGCAAA 
GACACCTGGT TACCAAGAAG CCATACATGT TTTAATCGCT TGGATCTACC ACCATATAAG 
AGTTATGAAC AACTAAAGGA AAAACTTCTT TTTGCAATAG AAGAGACAGA GGGATTTGGA 
CAAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Atypical ubiquitination by E3 ligase WWP1 inhibits the proteasome-mediated degradation of mutant huntingtin.
Brain research1643103-12(2016 07)
Lin L,Jin Z,Tan H,Xu Q,Peng T,Li H

Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis.
Journal of molecular and cellular cardiology881-13(2015 Nov)
Basheer WA,Harris BS,Mentrup HL,Abreha M,Thames EL,Lea JB,Swing DA,Copeland NG,Jenkins NA,Price RL,Matesic LE

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Wwp1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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