Espl1 cDNA ORF clone, Mus musculus(house mouse)

The following Espl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Espl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu16569 XM_006520259.4
Latest version!
Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu16569 XM_011245396.3
Latest version!
Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu16569 XM_006520258.4
Latest version!
Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu16569 NM_001356312.1
Latest version!
Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu16569 NM_001014976.2
Latest version!
Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu16569 XM_006520257.3
Latest version!
Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OMu16569 XM_006520259.3 Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu16569 XM_006520258.3 Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu16569 XM_011245396.2 Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu16569
    Clone ID Related Accession (Same CDS sequence) XM_006520259.3 , XM_011245396.3 , XM_006520258.3 , XM_006520259.4 , XM_006520257.3 , XM_006520258.4 , NM_001356312.1 , XM_011245396.2 , NM_001014976.2
    Accession Version XM_011245396.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245396.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011243698.1
    CDS516..6872
    Translation

    Target ORF information:

    RefSeq Version XM_011245396.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011245396.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16569
    Clone ID Related Accession (Same CDS sequence) XM_006520259.3 , XM_011245396.3 , XM_006520258.3 , XM_006520259.4 , XM_006520257.3 , XM_006520258.4 , NM_001356312.1 , XM_011245396.2 , NM_001014976.2
    Accession Version XM_006520259.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520259.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006520322.1
    CDS201..6557
    Translation

    Target ORF information:

    RefSeq Version XM_006520259.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006520259.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16569
    Clone ID Related Accession (Same CDS sequence) XM_006520259.3 , XM_011245396.3 , XM_006520258.3 , XM_006520259.4 , XM_006520257.3 , XM_006520258.4 , NM_001356312.1 , XM_011245396.2 , NM_001014976.2
    Accession Version XM_006520258.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520258.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006520321.1
    CDS425..6781
    Translation

    Target ORF information:

    RefSeq Version XM_006520258.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006520258.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16569
    Clone ID Related Accession (Same CDS sequence) XM_006520259.3 , XM_011245396.3 , XM_006520258.3 , XM_006520259.4 , XM_006520257.3 , XM_006520258.4 , NM_001356312.1 , XM_011245396.2 , NM_001014976.2
    Accession Version NM_001356312.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Mus musculus(house mouse)
    Product separin
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163291.3. On Sep 29, 2017 this sequence version replaced XM_006520257.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145844.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001343241.1
    CDS187..6543
    Translation

    Target ORF information:

    RefSeq Version NM_001356312.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001356312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16569
    Clone ID Related Accession (Same CDS sequence) XM_006520259.3 , XM_011245396.3 , XM_006520258.3 , XM_006520259.4 , XM_006520257.3 , XM_006520258.4 , NM_001356312.1 , XM_011245396.2 , NM_001014976.2
    Accession Version NM_001014976.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Mus musculus(house mouse)
    Product separin
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163291.3. On May 22, 2009 this sequence version replaced NM_001014976.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC082603.1, AY588413.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001014976.1
    CDS133..6489
    Translation

    Target ORF information:

    RefSeq Version NM_001014976.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001014976.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16569
    Clone ID Related Accession (Same CDS sequence) XM_006520259.3 , XM_011245396.3 , XM_006520258.3 , XM_006520259.4 , XM_006520257.3 , XM_006520258.4 , NM_001356312.1 , XM_011245396.2 , NM_001014976.2
    Accession Version XM_006520259.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520259.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006520322.1
    CDS203..6559
    Translation

    Target ORF information:

    RefSeq Version XM_006520259.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006520259.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16569
    Clone ID Related Accession (Same CDS sequence) XM_006520259.3 , XM_011245396.3 , XM_006520258.3 , XM_006520259.4 , XM_006520257.3 , XM_006520258.4 , NM_001356312.1 , XM_011245396.2 , NM_001014976.2
    Accession Version XM_006520258.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006520321.1
    CDS427..6783
    Translation

    Target ORF information:

    RefSeq Version XM_006520258.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006520258.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16569
    Clone ID Related Accession (Same CDS sequence) XM_006520259.3 , XM_011245396.3 , XM_006520258.3 , XM_006520259.4 , XM_006520257.3 , XM_006520258.4 , NM_001356312.1 , XM_011245396.2 , NM_001014976.2
    Accession Version XM_006520257.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product separin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006520320.1
    CDS228..6584
    Translation

    Target ORF information:

    RefSeq Version XM_006520257.3
    Organism Mus musculus(House mouse)
    Definition Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006520257.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16569
    Clone ID Related Accession (Same CDS sequence) XM_006520259.3 , XM_011245396.3 , XM_006520258.3 , XM_006520259.4 , XM_006520257.3 , XM_006520258.4 , NM_001356312.1 , XM_011245396.2 , NM_001014976.2
    Accession Version XM_011245396.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product separin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011243698.1
    CDS518..6874
    Translation

    Target ORF information:

    RefSeq Version XM_011245396.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus extra spindle pole bodies 1, separase (Espl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011245396.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGAGGAACT TCAAAGGAGT CAACTTCGCC ACTCTGCTCT GCAGCAAGGA GGAGACCCAG 
    CAGCTGCTGC CTGACTTGAA GGAGTTCCTG TCCAGATCCC GGACTGATTT CCCCAGCAGC
    CGAACAGATG CCGAAAGAAG ACAAATTTGC GATACCATCC TGAGGGCTTG CACCCAGCAG
    TTGACTGCCA AGCTCGATTG CCCTGGGCAC CTGAGGAGTA TTTTGGACCT GGCAGAGCTG
    GCCTGTGATG GCTATTTGTT GTCTACTCCA CAGCGCCCTC CCCTCTACCT GGAACGCATT
    CTCTTCATCC TACTTCGCAA TGGTTCCACT CAGGGAAGCC CAGACACTGT GCTGCGCCTT
    GCCCAGCCTC TCCATGCCTG CTTGGTTCAG AACTCTGGAG AGGCTGCCCC TCAGGACTAT
    GAGGCCGTGA CCCGGGGCAG CTTCTCTCTG TTTTGGAAGG GAGCAGAGGC ACTGTTGGAG
    CGGCGAGCTG CGTTCTCAAC TCGGCTGAAT GCCTTGAGCT TCTTGGTGCT CTTGGAGGAT
    GGCAGTGTCC CCTGTGAGGT CCCTCACTTT GCTTCTCCAA CCGCCTGTCG TTTAGTTGCC
    GCCTATCAGC TGTACGATGC TACAGGACAA GGTCTAGATG AAGCTGATGC CGACTTCCTG
    TATGAAGTGC TTTCTAGGCA CCTGATCAGA GTCTTAGTAG GTGAGGGTGG TAGCTCTCCC
    GGTCCTCTCT CGCCTCAGAG GGCCCTCTGC CTCTTGGAAA TCACCCTGGA ACACTGCCGT
    CGGCTCTGCT GGAACCACCA CCACCGCCAG GCTGCCAGAG CAGTGGAGAG GGCTCGTAAT
    CACCTAGAGA AGACCAGTGT AGCTCCTAGC CTCCAGTTAT GCCAGATGGG GGTTGAGCTG
    TTGGAAGCTG TAGAAGAAAG ACCAGGGGCA GTGGCCCAGC TTCTGAGGAA GGCAGCAGCC
    GTTCTTATCA ACAGTATCGA GGCGCCGTCG CCCCCGCTCC GGGCATTGTA CGACAGCTGC
    CAGTTCTTCC TTTCAGGCCT GGAGAGAGGC ATCAGGAGGC ACTGTGGACT TGATGCCATA
    TTGAGTCTCT TTGCTTTTCT TGGGGGATAC AGCTCCCTTG TCCGGCACCT GAGAGAAGTC
    TCTGAAGCAT CTTCCAAGCA GCAGCAGTGC TTGCTTCAGA TGCACTTCCA GGGTTTCCAC
    CTCTTCACCG GGATTGTGTA TGACTTTGCT CAAGGCTGTC AGGCAACTGA GCTTGCTCAG
    CTAGTGGACG GTTGCAGATC TGCTGCTGTC TGGATGTTGG AGGCCTTAGA GGGCCTGTCG
    GGTGGAGAGC TGGCTGACTA CCTGAGCATG ACTGCCTCTT ACACCAGCAA CTTGGCCTAC
    AGCTTCTTTA GTCAGAAGCT CTATGAGGAG GCCTGCGTCA TCTCAGAGCC AGTCTGTCAG
    CACTTGGGCT CGGCAACGTC GGGTGCTTGT CCTGAAGTGC CTCCTGAGAA GCTACATAGG
    TGCTTCAGGC TGCATGTGGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG
    ATGGTGACCT TATGGCTGGC AGCTCTGAAA CCCTACAGTC TGGAACACAT GGTCGAGCCA
    GTCACTTTCT GGGTTCGAGT CAAAATGGAT GCATCCAGGG CAGGAGACAA GGAGCTACAA
    CTTCAGACTT TGCGAGACAG CCTAAGCTGC TGGGACCCAG AGACACAGTC TCTGCTTCTC
    AGGGAGGAGC TGCGGGCCTA CAAGTCAGTA CGGGCTGACA CTGGGCAGGA GCGCTTCAAC
    ATCATCTGTG ATCTGCTCGA GCTGAGCCCT GAAGAGACAG CAGCTGGAGC CTGGGCACGA
    GCTACCTACC TGGTGGAACT GGCCCAAGTG CTGTGCTACC ACAACTTTAC CCAGCAGACC
    AACTGCTCTG CCTTGGATGC TGTCCAGGAA GCCCTGCAGC TTCTGGAGTC CGTGAGTCCT
    GAGGCCCAGG AGCAGGATCG ACTTCTGGAT GATAAAGCAC AGGCCTTGTT ATGGCTTTAC
    ATCTGTACCT TAGAGGCCAA GATGCAGGAA GGTATTGAGC GGGATCGGAG AGCCCAGGCC
    CCTAGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTGCAGGAA
    GATCGTTTTC TGTATAGTAG CATTGCCTTC AATCTGGCTG CTGATGCAGC TCAGTCCAAA
    TGCCTGGACC AGGCCCTGAC TCTGTGGAAA GAAGTGCTCA CCAAGGGGCG AGCCCCAGCT
    GTGCGGTGTC TCCAGCAGAC TGCAGCCTCC CTGCAAATCC TAGCGGCCGT CTATCAGTTG
    GTGGCAAAGC CCTTGCAGGC TCTGGAGACC CTCCTGCTCC TGCAGATTGT ATCTAAGAGA
    CTGCAGGACC ACGCAAAGGC AGCCAGCTCT TCCTGCCAGC TTACCCAGCT ACTCTTGAAC
    CTTGGCTGTC CCAGCTATGC GCAGTTGTAT CTGGAAGAGG CGGAGTCAAG CCTGAGGAGC
    CTGGACCAGA CAAGTGACGC GTGCCAGCTG CTGTCCCTGA CCTGTGCTCT GCTTGGAAGT
    CAGCTCTGCT GGGCTTGCCA GAAGGTAACT GCAGGCGTTT CTTTGTTGCT TTCTGTGCTT
    CGGGATCCTG CCCTCCAGAA GTCATCTAAG GCTTGGTACT TGCTACGTGT CCAGGCCCTG
    CAAGTTCTGG CTTTTTACCT CAGTCTCTCG TCCAACCTCC TCTCAAGTGC CCTGCGTGAG
    CAGCTCTGGG ATCAAGGCTG GCAGACCCCG GAGACGGCGC TGATTGATGC CCACAAGCTC
    CTCAGAAGCA TCATCATTCT GCTCATGGGA AGTGATGTGC TCTCCATTCA GAAAGCAGCC
    ACCGAGTCGC CCTTTCTGGA CTACGGTGAG AATCTGGTGC AAAAATGGCA GGTTCTCACA
    GAGGTGCTGA CCTGCTCGGA GAGGCTGGTC GGCCGCCTGG GTCGCCTGGG GAACGTGAGT
    GAGGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACGA CTAAGCTGCA GATACCTCGA
    CAGTGTGCCC TGTTCCTTGT ACTGAAGGGT GAGCTGGAGC TGGCCCGGGG TGACATTGAC
    CTCTGTCAGT CAGACTTGCA GCAGGTTCTC TTCTTGCTCG AGTCTTCCAC AGAGTTTGGT
    GTTGTGACCC AGCATCCAGA CTCAGTGAAG AAGGTGCACA CGCAGAAAGG GAAGCATAAG
    GCTCAAGGCC CATGTTTCCC ACCCCTGTCA GAGGAGGAGC CCTTCCTGAA AGGTCCTGCC
    CTTGAGCTGG TGGACACTGT GCTCAACGAA CCTGGTCCCA TCCAGTCCTC TGTAAACTCC
    TCCCCAGTCC TGAAAACCAA GCCACCACCC AACCCTGGCT TCCTGTCTCA CTTGCCCAGC
    TGTGACTGCT TACTGTGTGC CAGCCCTGCC CTCTCAGCAG TCTGTCTGCG CTGGGTGCTG
    GTCACTGCCG GAGTGAGGCT GGCCACCGGT CACAAAGCTC AGGGTCTGGA TCTGCTGCAA
    GCTGTGCTGA CGCGTTGCCC TGCAGCCACC AAGCGCTTCA CACAGAGTCT CCAAGCTTCT
    CTGAATCACA GAACAACCCC CTCTTGTGTT CCCAGCCTCT TTGATGAGAT CATGGCTCAA
    GTGTACACGC ACTTGGCATT GGAGTTCCTG AATCAGACAT CTGAAAAGAG CCTGGGGAAG
    GTCCTGGCGT CGGGGCTGAA GTTTGTGGCA ACACGGATAC AGAGCTTGGA GATCTGGAGA
    GCCCACCTGC TCTTGGTTCA GGCCCTTGCA AAGCTGGCCC ACTTCAGCTG CTGTACTTCC
    GAACTCTTTG CAAGCTCCTG GGGCTGGCAC CCACCATTAG TAAAAAGTCT GCCAGTCTTA
    GAGCCTGCTA AAATTCGGAG ACAAAAATGC TCTGGACGGG GCCGCCGGCG GATAGCCTCT
    GTTCCCCCAC CCCTCCATAA CAGCTCTCAG AAAGGTCTGG AAGAGGAAGG GCCACCTTGT
    ACCCCTAAGC CTCCAGGTCG GGCTAGGCAA GCTGGCCCTC GTGTCCCTTT CACCATATTT
    GAAGAAGTCC ACCCCACCAA GAGCAAGCTC CAGGTTCCCT TGGCTCCCAG GGTCCACAGG
    CGGGCCCAGA CTCGCCTCAA GGTGATCTTC AGTGATGACA GTGACCTGGA AGACCTTGTC
    TCAGCTGACA CACAACTTGT AGAGGAGCCT AAGAGGCGAG GGACTGCTTC CCGTACCCGG
    GGCCAAACTA GGAAGGGACG TAGCTTAAAG ACAGATGCGG TGGTTGCTAT AGAAAGTACC
    CCTGGACATT CCAGTGTCAG TGGGAGGACT CGGAGGGCCA GGAAGGTGGC TTCAAGAAAT
    TGTGAAGAAG AGAGTCCCAA AGCACCCCTC TGTGTCTGGG CCAGCCAGGG ACCCGAGATC
    ATGAGAAGTA TCCCTGAAGA GGAGCCAGTG GACAACCATC TGGAAAAAAG CTTCGAGATT
    CTCAGGGGTT CTGATGGGGA AGACTCGGCC TCAGGTGAGA AGGCGGCAGC TGCAGACACT
    GGTCTACCCG TAGGAGAGTG TGAGGTGTTG AGACGGGACT CCAGCAAGGC AGAGAGACCT
    GTCTTGTACT CAGACACGGA GGCCAATAGT GACCCTAGTC CTTGGCTCCC ACCCTTCTCA
    GTTCCTGCAC CCATTGATCT TTCTACCCTG GATTCCATTT CTGACTCATT GAGCATTGCT
    TTCCGTGGTG TTAGTCACTG CCCTCCAAGT GGGCTCTATG CTCACCTCTG CCGCTTCTTG
    GCCCTGTGTC TGGGCCACCG AGATCCTTAT GCCACTGCGT TCCTTGTTGC TGAGTCTATC
    TCCATCACCT GCCGTCACCA GCTGCTCACC CACTTGCACC GACAGCTCAG CAAGGCCCAG
    AAGCAGCAAG AATCACCTGA ACTGGCAGAG CATCTGCAGA GGCTGGACCT GAAGGAGAGG
    CCCGGAGGTG TCCCACTGGC CCGTATCCAG CGCCTCTTTT CCTTTAAGGC TTTGGGATCT
    GGCTGCTTCC CCCAGGCAGA GAAGGAGAGT TTCCAGGAGC GCCTGGCTCT GATCCCTAGT
    GGGGTGACTG TCTGTGTGTT GGCTCTGGCC ACCCTCCAGC CTGGAACCTT GAGCAACACT
    CTTTTACTGA CCCGCCTGGA AAAGGACAAC CCCCCAATTA CTGTGAAGAT CCCCACTGCC
    CAAAATAAGC TCCCTCTAAG CGCAGTGCTG AAAGAGTTTG ATGCCATCCA GAAGGACCAA
    AAAGAAAACA GCAGCTGTAC TGAGAAACGA GTATGGTGGA CGGGCCGGCT GGCACTGGAC
    CAAAGGATGG AGGCACTCAT CACCGCCCTG GAGGAACAGG TGCTGGGCTG CTGGAGGGGG
    CTGCTGCTGC CATGCAGTGC TGACCCCAGC CTTGCCCAGG AGGCCTCCAA ACTACAAGAG
    CTGCTGCGGG AATGTGGCTG GGAATATCCT GACTCCACTC TCCTGAAAGT CATCCTTAGT
    GGGGCCAGAA TCCTCACCAG CCAGGATGTT CAGGCCTTGG CGTGTGGGCT GTGCCCAGCC
    CAGCCTGACC GAGCCCAAGT GCTCCTGAGC GAGGCAGTAG GACAGGTTCA GAGCCAGGAG
    GCCCCTCGAA GTCAACATCT TGTATTGGTG TTAGACAAGG ACCTGCAAAA GCTGCCTTGG
    GAAAGCACGC CCATCCTCCA AGCACAGCCG GTCACCCGGC TGCCCTCCTT CCGCTTCCTG
    CTCAGCTACA CAGTCACTAA AGAGGCTGGA GCCTCATCAG TGCTGAGCCA AGGTGTTGAT
    CCACAGAATA CCTTCTATGT GCTAAACCCT CACAGTAACC TGTCGAGCAC AGAGGAGAGA
    TTTCGAGCCA GTTTTAGCAG TGAAACTGGC TGGAAAGGAG TGATTGGGGA AGTGCCAAGC
    CTCGATCAGG TGCAGGCTGC CCTGACAGAG CGGGATTTAT ATATCTACGC AGGGCACGGA
    GCCGGTGCCC GCTTCCTTGA TGGGCAGGCT GTCCTGCGGC TGAGCTGCCG GGCGGTGGCC
    CTGCTGTTCG GCTGCAGCAG TGCAGCCCTT GCCGTGCATG GAAACCTGGA GGGAGCTGGT
    ATCGTGCTCA AGTACATCAT GGCTGGCTGC CCCTTGTTTC TAGGTAATCT CTGGGATGTG
    ACTGACCGTG ACATTGACCG TTACACAGAG GCTTTGCTGC AGGGCTGGCT GGGAGCAGGC
    CCAGGGGCTC CCTTTCTCTA CTATGCCAGC CAAGCCCGCC AGGCCCCTCG ACTCAAGTAC
    CTCATTGGTG CTGCACCTGT AGCCTACGGC CTACCTATCT CTCTGCAGAC ACCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.