Nckap1l cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nckap1l
Entrez Gene ID	105855
Full Name	NCK associated protein 1 like
Synonyms	4930568P13Rik,AI463083,Hem1,Hemp1
General protein information	Preferred Names NCK associated protein 1 like Names nck-associated protein 1-like hematopoietic protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_153505.4	NP_705725.1	nck-associated protein 1-like
XM_006520255.3	XP_006520318.2	nck-associated protein 1-like isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NCKAP1L	NP_005328.2
Pan troglodytes (chimpanzee)	NCKAP1L	XP_509114.3
	NCKAP1L	XP_002798677.1
Bos taurus (cattle)	NCKAP1L	NP_001137348.1
Mus musculus (house mouse)	Nckap1l	NP_705725.1
Rattus norvegicus (Norway rat)	Nckap1l	NP_001101589.1
Danio rerio (zebrafish)	zgc:172352	NP_001107884.1
Gallus gallus (chicken)	NCKAP1L	NP_001026510.1
Xenopus tropicalis (tropical clawed frog)	nckap1l	XP_002935704.2
Canis lupus familiaris (dog)	NCKAP1L	XP_005636795.1

Gene Ontology
Bibliography

Related articles in PubMed

A functional genomics predictive network model identifies regulators of inflammatory bowel disease.
Peters LA, Perrigoue J, Mortha A, Iuga A, Song WM, Neiman EM, Llewellyn SR, Di Narzo A, Kidd BA, Telesco SE, Zhao Y, Stojmirovic A, Sendecki J, Shameer K, Miotto R, Losic B, Shah H, Lee E, Wang M, Faith JJ, Kasarskis A, Brodmerkel C, Curran M, Das A, Friedman JR, Fukui Y, Humphrey MB, Iritani BM, Sibinga N, Tarrant TK, Argmann C, Hao K, Roussos P, Zhu J, Zhang B, Dobrin R, Mayer LF, Schadt EE
Nature genetics49(10)1437-1449(2017 Oct)

Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of?leukocytes.
Leithner A, Eichner A, M?ller J, Reversat A, Brown M, Schwarz J, Merrin J, de Gorter DJ, Schur F, Bayerl J, de Vries I, Wieser S, Hauschild R, Lai FP, Moser M, Kerjaschki D, Rottner K, Small JV, Stradal TE, Sixt M
Nature cell biology18(11)1253-1259(2016 Nov)

Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments.
Thiam HR, Vargas P, Carpi N, Crespo CL, Raab M, Terriac E, King MC, Jacobelli J, Alberts AS, Stradal T, Lennon-Dumenil AM, Piel M
Nature communications710997(2016 Mar)

Hematopoietic protein-1 regulates the actin membrane skeleton and membrane stability in murine erythrocytes.
Chan MM, Wooden JM, Tsang M, Gilligan DM, Hirenallur-S DK, Finney GL, Rynes E, Maccoss M, Ramirez JA, Park H, Iritani BM
PloS one8(2)e54902(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a novel, critical role for Hem-1 in the homeostasis of structural proteins required for formation and stability of the actin membrane skeleton in erythrocytes
Title: Hematopoietic protein-1 regulates the actin membrane skeleton and membrane stability in murine erythrocytes.

results demonstrate that Hem1 is essential for hematopoietic cell development, function, and homeostasis by controlling a distinct pathway leading to cytoskeletal reorganization
Title: A point mutation in the murine Hem1 gene reveals an essential role for Hematopoietic protein 1 in lymphopoiesis and innate immunity.

The following Nckap1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nckap1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02470	NM_153505.4 Latest version!	Mus musculus NCK associated protein 1 like (Nckap1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu02470	XM_006520255.3 Latest version!	Mus musculus NCK associated protein 1 like (Nckap1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nckap1l ( NM_153505.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705725.1 Mus musculus NCK associated protein 1 like (Nckap1l), mRNA.

Price & Availability↑

CloneID	OMu02470
Clone ID Related Accession (Same CDS sequence)	NM_153505.4 , XM_006520255.3
Accession Version	NM_153505.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	nck-associated protein 1-like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC131721.4. On May 1, 2007 this sequence version replaced NM_153505.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK083390.1, SRR1660813.78233.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGTCTTTGA CCTCTGCCTA TCAGCTTAAA TTAGCAGAGA AGCTCACGAT CCTGAATGAC 
CGAGGACAGG GAGTTCTCAT CCGCATGTAC AACATCAAGA AGACCTGTTC AGATTCAAAA 
TCCAAGCCAC CTTTTTTACT GGAAAAGTCC ATGGAATCAT GTCTCAAGTA CATCAACAAG 
AAGTTTCCCA ACATAGATGT CCGAAATAGC ACGCAACATT TAGGACCAGT GCATCGTGAA 
AAAGCTGAGA TAATTAGATT CCTCACTAAC TATTACCAGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTATATGA ACTTCTCAAC ACCATTGATG CCTGCCAGTG TCATTTTGAT 
ATTAATCTCA ACTTTGACTT CACTCGGAGC TACCTGGACT TAATTGTGAC GTACACCTCA 
GTCATTTTAC TCCTGTCACG GATTGAGGAC CGCAGGATAC TCATTGGCAT GTACAACTGT 
GCCCATGAGA TGCTGCATGG GCACAGTGAC CCCAGTTTTG CTCGCCTGGG CCAGATGGTA 
TTAGAGTATG ACCATCCTCT GAAGAAGCTG ACAGAAGAAT TTGGACCTCA CACAAAGGCT 
GTGAGTGGAG CCCTGCTCTC TCTGCACTTC CTCTTTGTTC GGAGGAACCA GGGAGCTGAG 
CAGTGGCGCA GCGCCCAGCT TCTAAGCCTC ATCAGCAGCC CCCCGGCCAT GATTAACCCT 
GCTAATTCAG ACACAATGGC CTGTGAGTAT CTGTCCGTGG AAGTGATGGA GCGATGGATC 
ATAATTGGGT TTCTTCTTTG CCATGGGTGC CTCAACTCCA ACAGCCAGTG CCAGAAGCTG 
TGGAAGCTGT GTCTGGAGGG TTCCCTGTAC ATCACGCTCA TCCGGGAAGA TGTGCTGCAA 
GTGCACAAGG TCACCGAGGA CCTGTTCAGC AGTTTGAAAG GGTACAGTAA GCGAGTGGCA 
GACATCAAGG AGAGCAAGGA ACACGCCATT ACAAACAGTG GCCAGTTTCA CTGTCAACGC 
CGGCAGTTTC TGCGGACAGC TGTGAAGGAG TTGGAGACTG TGTTAAATGA TGAACCAGGC 
CTGCTTGGTC CTAAGGCCCT TTTTGCTTTC ATGGCCCTGT CTTTCATTCG TGATGAGGTC 
ACTTGGCTGG TTCGCCATAC AGAAAATGTC ACCAAGACTA AGACACCCGA AGACTATGCT 
GACTCGAGCA TTGCAGAGCT TCTGTTCCTG TTGGAGGAGA TCAGGGCTCT GGTCAGAAGA 
CACATCAAAG TTATTCAGCA GTACCACCTC CAGTACCTGG CCAGATTTGA TGTCCTTGTG 
CTTAGTGACA TCATTCAGAA TCTGTCCGTG TGTCCAGAGG AGGAGTCTGT CATTATGTCC 
TCATTTGTTA GTATACTGTC CTCTCTGAAT CTCAAGCAAG TTGATAATGA AGAGAAATTT 
GACTTTTCTG GATTAAGGCT GGATTGGTTT CGCCTACAGG CATATACCAG CGTGTCGAAG 
GCACCTTTGC ACCTGCATGA GAACCCTGAC CTAGCCAAGG TCATGAACCT CATCATCTTT 
CACTCCCAGA TGTTGGACTC AGTAGAGAAA ATGCTGGTGG AAACCTCTGA CCTTTCTACT 
TTCTGCTTTC ATCTCCGTAC CTTTGAGAAG ATGTTTGCTA CGACCCTGGA GGAACCAACC 
ATGCTTCGCT ACACCATTGC CTTCCCCCTG ATTTGTGCTC ATTTTGTCCA CTGCGTTCAT 
GAAATGTGCC CAGAGGAGTA CCCTCACCTG AAGAACCATG GTCTTCACCA CTGCAACTCC 
TTCCTGGAAG ATCTGGCCAA GCAGACCAGC AACTGTGTGC TTGAGATATG TGCGGAACAG 
CGGAACCTGA ATGAACAGCT TCTACCTAAG CACTGTGCCA CCACCATCAG CAAAGCCAAG 
AATAAGAAGT CCATGAAGCA GAGGCAGGCT CCCAGGAAAG GAGAGCCTGA GAGGGACAAG 
CCAGGAGCAG AGAGTCATCG GAAGAACCGC AGCCTTGTCA CTAACATGGA CAAACTACAT 
CTAAACTTGA CTGAATTGGC ACTGGCAATG AATCATGTGC ACAGTTTCTC TGTGTTTGAA 
CACACCATCT TCCCATCTGA GTATCTCAGC AGTCACCTGG AGGCCAGGCT TAACAGAGCC 
ATCGTGACTT TGGCTGGCTA CAATGCCACA ACCCAGGAGA TCCTTAGGCC TTCTGAGCTG 
CTGGCAGGAG TCAAAGCATA CATCAGTTTC ATCCAGTCAT TGGCACAGTT TTTGGGTGCA 
GATGCTTCCA GAATCGTTCG CAATGCCCTC CTGCAGCAGA CACAGCCACT GGACTCATCT 
GGCGAGCAGA CTGTCACCAC TCTCTACACA AACTGGTACC TAGAAAGTCT CCTTAGACAG 
GCGAGCAGTG GAGCCATTGT CCTCTCCCCA GCCATGCAGG CCTTCATCAG CCTTCCCCGA 
GATGGAGAGC AGAACTTCAG TGCTGAGGAG TTCTCGGACA TCTCTGAGAT GCGAGCCTTG 
GCTGAAATTC TTGGTCCCTA TGGCATGAAG TTCCTGAGTG AAAACCTGAT GTGGCATGTG 
ACCTCTCAGA TTGTGGAGCT GAAGAAGCTT GTGGTGGAAA ACATGGATAT TCTGGTCCAG 
ATCAGATCCA ACTTCAGCAA GCCAGAATTA ATGGCATCTC TGCTGCCCCA GCTGACAGGA 
GCTGAAAATG TGCTGAAACG AATGACCATC ATTGGGGTGA TTCTCAGTTT TAGGGCCATG 
GCCCAAGAGG GACTTCAGGA GGTTTTTTCT GCCCACTGCC CTTTTCTCAT GGGCCCCATT 
GAGTGCCTGA AGGAGTTTGT CACCCCTGAC ACAGACATTA AGGTCACCCT GAGTGTCTTT 
GAGCTGGCAT GTGCTGCAGG GGTGAGCTGT GACATTGACC CAGCCTTGGT AGCTGCCATT 
GCCAATCTGA AAGCTGATAA CTCATCCCCT GAAGAAGAGT ATAAGGTGGC ATGCCTACTC 
TTGATCTTTC TTGCTGTTTC CCTCCCACTC CTTGCCACTG ACCCGTCTTC CTTCTTCAGC 
ATTGAGAAAG ATGGCTACAA CAACAACATC CACTGTTTGA CCAAAGCCAT CATTCAGGTG 
TCTGCTGCCC TCTTCACTCT GTACAACAAG AACATTGAAA CGCACCTCAA AGAGTTTCTG 
GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG GGCCAGGAGA CTGACAAGCT CAAAACCAGA 
AATCGTGAAT CCATTTCTCT GCTCATGCGC TTGGTGGTGG AGGAGTCACC CTTCTTGACC 
CTGGACATGC TGGAGTCCTG TTTCCCTTAC GTTCTGCTAC GCAATGCCTA TCGGGAGGTT 
TCCCGGGCCT TCTACTTGAA CCGTCTGCCA GCCAGTTCCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705725.1
CDS	56..3460
Translation	MSLTSAYQLKLAEKLTILNDRGQGVLIRMYNIKKTCSDSKSKPPFLLEKSMESCLKYINKKFPNIDVRNSTQHLGPVHREKAEIIRFLTNYYQSFVDVMEFRDHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTSVILLLSRIEDRRILIGMYNCAHEMLHGHSDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHFLFVRRNQGAEQWRSAQLLSLISSPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQCQKLWKLCLEGSLYITLIREDVLQVHKVTEDLFSSLKGYSKRVADIKESKEHAITNSGQFHCQRRQFLRTAVKELETVLNDEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTKTKTPEDYADSSIAELLFLLEEIRALVRRHIKVIQQYHLQYLARFDVLVLSDIIQNLSVCPEEESVIMSSFVSILSSLNLKQVDNEEKFDFSGLRLDWFRLQAYTSVSKAPLHLHENPDLAKVMNLIIFHSQMLDSVEKMLVETSDLSTFCFHLRTFEKMFATTLEEPTMLRYTIAFPLICAHFVHCVHEMCPEEYPHLKNHGLHHCNSFLEDLAKQTSNCVLEICAEQRNLNEQLLPKHCATTISKAKNKKSMKQRQAPRKGEPERDKPGAESHRKNRSLVTNMDKLHLNLTELALAMNHVHSFSVFEHTIFPSEYLSSHLEARLNRAIVTLAGYNATTQEILRPSELLAGVKAYISFIQSLAQFLGADASRIVRNALLQQTQPLDSSGEQTVTTLYTNWYLESLLRQASSGAIVLSPAMQAFISLPRDGEQNFSAEEFSDISEMRALAEILGPYGMKFLSENLMWHVTSQIVELKKLVVENMDILVQIRSNFSKPELMASLLPQLTGAENVLKRMTIIGVILSFRAMAQEGLQEVFSAHCPFLMGPIECLKEFVTPDTDIKVTLSVFELACAAGVSCDIDPALVAAIANLKADNSSPEEEYKVACLLLIFLAVSLPLLATDPSSFFSIEKDGYNNNIHCLTKAIIQVSAALFTLYNKNIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESPFLTLDMLESCFPYVLLRNAYREVSRAFYLNRLPASSH

Target ORF information:

RefSeq Version	NM_153505.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK associated protein 1 like (Nckap1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153505.4

ORF Insert Sequence:

ATGTCTTTGA CCTCTGCCTA TCAGCTTAAA TTAGCAGAGA AGCTCACGAT CCTGAATGAC 
CGAGGACAGG GAGTTCTCAT CCGCATGTAC AACATCAAGA AGACCTGTTC AGATTCAAAA 
TCCAAGCCAC CTTTTTTACT GGAAAAGTCC ATGGAATCAT GTCTCAAGTA CATCAACAAG 
AAGTTTCCCA ACATAGATGT CCGAAATAGC ACGCAACATT TAGGACCAGT GCATCGTGAA 
AAAGCTGAGA TAATTAGATT CCTCACTAAC TATTACCAGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTATATGA ACTTCTCAAC ACCATTGATG CCTGCCAGTG TCATTTTGAT 
ATTAATCTCA ACTTTGACTT CACTCGGAGC TACCTGGACT TAATTGTGAC GTACACCTCA 
GTCATTTTAC TCCTGTCACG GATTGAGGAC CGCAGGATAC TCATTGGCAT GTACAACTGT 
GCCCATGAGA TGCTGCATGG GCACAGTGAC CCCAGTTTTG CTCGCCTGGG CCAGATGGTA 
TTAGAGTATG ACCATCCTCT GAAGAAGCTG ACAGAAGAAT TTGGACCTCA CACAAAGGCT 
GTGAGTGGAG CCCTGCTCTC TCTGCACTTC CTCTTTGTTC GGAGGAACCA GGGAGCTGAG 
CAGTGGCGCA GCGCCCAGCT TCTAAGCCTC ATCAGCAGCC CCCCGGCCAT GATTAACCCT 
GCTAATTCAG ACACAATGGC CTGTGAGTAT CTGTCCGTGG AAGTGATGGA GCGATGGATC 
ATAATTGGGT TTCTTCTTTG CCATGGGTGC CTCAACTCCA ACAGCCAGTG CCAGAAGCTG 
TGGAAGCTGT GTCTGGAGGG TTCCCTGTAC ATCACGCTCA TCCGGGAAGA TGTGCTGCAA 
GTGCACAAGG TCACCGAGGA CCTGTTCAGC AGTTTGAAAG GGTACAGTAA GCGAGTGGCA 
GACATCAAGG AGAGCAAGGA ACACGCCATT ACAAACAGTG GCCAGTTTCA CTGTCAACGC 
CGGCAGTTTC TGCGGACAGC TGTGAAGGAG TTGGAGACTG TGTTAAATGA TGAACCAGGC 
CTGCTTGGTC CTAAGGCCCT TTTTGCTTTC ATGGCCCTGT CTTTCATTCG TGATGAGGTC 
ACTTGGCTGG TTCGCCATAC AGAAAATGTC ACCAAGACTA AGACACCCGA AGACTATGCT 
GACTCGAGCA TTGCAGAGCT TCTGTTCCTG TTGGAGGAGA TCAGGGCTCT GGTCAGAAGA 
CACATCAAAG TTATTCAGCA GTACCACCTC CAGTACCTGG CCAGATTTGA TGTCCTTGTG 
CTTAGTGACA TCATTCAGAA TCTGTCCGTG TGTCCAGAGG AGGAGTCTGT CATTATGTCC 
TCATTTGTTA GTATACTGTC CTCTCTGAAT CTCAAGCAAG TTGATAATGA AGAGAAATTT 
GACTTTTCTG GATTAAGGCT GGATTGGTTT CGCCTACAGG CATATACCAG CGTGTCGAAG 
GCACCTTTGC ACCTGCATGA GAACCCTGAC CTAGCCAAGG TCATGAACCT CATCATCTTT 
CACTCCCAGA TGTTGGACTC AGTAGAGAAA ATGCTGGTGG AAACCTCTGA CCTTTCTACT 
TTCTGCTTTC ATCTCCGTAC CTTTGAGAAG ATGTTTGCTA CGACCCTGGA GGAACCAACC 
ATGCTTCGCT ACACCATTGC CTTCCCCCTG ATTTGTGCTC ATTTTGTCCA CTGCGTTCAT 
GAAATGTGCC CAGAGGAGTA CCCTCACCTG AAGAACCATG GTCTTCACCA CTGCAACTCC 
TTCCTGGAAG ATCTGGCCAA GCAGACCAGC AACTGTGTGC TTGAGATATG TGCGGAACAG 
CGGAACCTGA ATGAACAGCT TCTACCTAAG CACTGTGCCA CCACCATCAG CAAAGCCAAG 
AATAAGAAGT CCATGAAGCA GAGGCAGGCT CCCAGGAAAG GAGAGCCTGA GAGGGACAAG 
CCAGGAGCAG AGAGTCATCG GAAGAACCGC AGCCTTGTCA CTAACATGGA CAAACTACAT 
CTAAACTTGA CTGAATTGGC ACTGGCAATG AATCATGTGC ACAGTTTCTC TGTGTTTGAA 
CACACCATCT TCCCATCTGA GTATCTCAGC AGTCACCTGG AGGCCAGGCT TAACAGAGCC 
ATCGTGACTT TGGCTGGCTA CAATGCCACA ACCCAGGAGA TCCTTAGGCC TTCTGAGCTG 
CTGGCAGGAG TCAAAGCATA CATCAGTTTC ATCCAGTCAT TGGCACAGTT TTTGGGTGCA 
GATGCTTCCA GAATCGTTCG CAATGCCCTC CTGCAGCAGA CACAGCCACT GGACTCATCT 
GGCGAGCAGA CTGTCACCAC TCTCTACACA AACTGGTACC TAGAAAGTCT CCTTAGACAG 
GCGAGCAGTG GAGCCATTGT CCTCTCCCCA GCCATGCAGG CCTTCATCAG CCTTCCCCGA 
GATGGAGAGC AGAACTTCAG TGCTGAGGAG TTCTCGGACA TCTCTGAGAT GCGAGCCTTG 
GCTGAAATTC TTGGTCCCTA TGGCATGAAG TTCCTGAGTG AAAACCTGAT GTGGCATGTG 
ACCTCTCAGA TTGTGGAGCT GAAGAAGCTT GTGGTGGAAA ACATGGATAT TCTGGTCCAG 
ATCAGATCCA ACTTCAGCAA GCCAGAATTA ATGGCATCTC TGCTGCCCCA GCTGACAGGA 
GCTGAAAATG TGCTGAAACG AATGACCATC ATTGGGGTGA TTCTCAGTTT TAGGGCCATG 
GCCCAAGAGG GACTTCAGGA GGTTTTTTCT GCCCACTGCC CTTTTCTCAT GGGCCCCATT 
GAGTGCCTGA AGGAGTTTGT CACCCCTGAC ACAGACATTA AGGTCACCCT GAGTGTCTTT 
GAGCTGGCAT GTGCTGCAGG GGTGAGCTGT GACATTGACC CAGCCTTGGT AGCTGCCATT 
GCCAATCTGA AAGCTGATAA CTCATCCCCT GAAGAAGAGT ATAAGGTGGC ATGCCTACTC 
TTGATCTTTC TTGCTGTTTC CCTCCCACTC CTTGCCACTG ACCCGTCTTC CTTCTTCAGC 
ATTGAGAAAG ATGGCTACAA CAACAACATC CACTGTTTGA CCAAAGCCAT CATTCAGGTG 
TCTGCTGCCC TCTTCACTCT GTACAACAAG AACATTGAAA CGCACCTCAA AGAGTTTCTG 
GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG GGCCAGGAGA CTGACAAGCT CAAAACCAGA 
AATCGTGAAT CCATTTCTCT GCTCATGCGC TTGGTGGTGG AGGAGTCACC CTTCTTGACC 
CTGGACATGC TGGAGTCCTG TTTCCCTTAC GTTCTGCTAC GCAATGCCTA TCGGGAGGTT 
TCCCGGGCCT TCTACTTGAA CCGTCTGCCA GCCAGTTCCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nckap1l ( XM_006520255.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520318.2 Mus musculus NCK associated protein 1 like (Nckap1l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02470
Clone ID Related Accession (Same CDS sequence)	NM_153505.4 , XM_006520255.3
Accession Version	XM_006520255.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	nck-associated protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520255.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTGA CCTCTGCCTA TCAGCTTAAA TTAGCAGAGA AGCTCACGAT CCTGAATGAC 
CGAGGACAGG GAGTTCTCAT CCGCATGTAC AACATCAAGA AGACCTGTTC AGATTCAAAA 
TCCAAGCCAC CTTTTTTACT GGAAAAGTCC ATGGAATCAT GTCTCAAGTA CATCAACAAG 
AAGTTTCCCA ACATAGATGT CCGAAATAGC ACGCAACATT TAGGACCAGT GCATCGTGAA 
AAAGCTGAGA TAATTAGATT CCTCACTAAC TATTACCAGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTATATGA ACTTCTCAAC ACCATTGATG CCTGCCAGTG TCATTTTGAT 
ATTAATCTCA ACTTTGACTT CACTCGGAGC TACCTGGACT TAATTGTGAC GTACACCTCA 
GTCATTTTAC TCCTGTCACG GATTGAGGAC CGCAGGATAC TCATTGGCAT GTACAACTGT 
GCCCATGAGA TGCTGCATGG GCACAGTGAC CCCAGTTTTG CTCGCCTGGG CCAGATGGTA 
TTAGAGTATG ACCATCCTCT GAAGAAGCTG ACAGAAGAAT TTGGACCTCA CACAAAGGCT 
GTGAGTGGAG CCCTGCTCTC TCTGCACTTC CTCTTTGTTC GGAGGAACCA GGGAGCTGAG 
CAGTGGCGCA GCGCCCAGCT TCTAAGCCTC ATCAGCAGCC CCCCGGCCAT GATTAACCCT 
GCTAATTCAG ACACAATGGC CTGTGAGTAT CTGTCCGTGG AAGTGATGGA GCGATGGATC 
ATAATTGGGT TTCTTCTTTG CCATGGGTGC CTCAACTCCA ACAGCCAGTG CCAGAAGCTG 
TGGAAGCTGT GTCTGGAGGG TTCCCTGTAC ATCACGCTCA TCCGGGAAGA TGTGCTGCAA 
GTGCACAAGG TCACCGAGGA CCTGTTCAGC AGTTTGAAAG GGTACAGTAA GCGAGTGGCA 
GACATCAAGG AGAGCAAGGA ACACGCCATT ACAAACAGTG GCCAGTTTCA CTGTCAACGC 
CGGCAGTTTC TGCGGACAGC TGTGAAGGAG TTGGAGACTG TGTTAAATGA TGAACCAGGC 
CTGCTTGGTC CTAAGGCCCT TTTTGCTTTC ATGGCCCTGT CTTTCATTCG TGATGAGGTC 
ACTTGGCTGG TTCGCCATAC AGAAAATGTC ACCAAGACTA AGACACCCGA AGACTATGCT 
GACTCGAGCA TTGCAGAGCT TCTGTTCCTG TTGGAGGAGA TCAGGGCTCT GGTCAGAAGA 
CACATCAAAG TTATTCAGCA GTACCACCTC CAGTACCTGG CCAGATTTGA TGTCCTTGTG 
CTTAGTGACA TCATTCAGAA TCTGTCCGTG TGTCCAGAGG AGGAGTCTGT CATTATGTCC 
TCATTTGTTA GTATACTGTC CTCTCTGAAT CTCAAGCAAG TTGATAATGA AGAGAAATTT 
GACTTTTCTG GATTAAGGCT GGATTGGTTT CGCCTACAGG CATATACCAG CGTGTCGAAG 
GCACCTTTGC ACCTGCATGA GAACCCTGAC CTAGCCAAGG TCATGAACCT CATCATCTTT 
CACTCCCAGA TGTTGGACTC AGTAGAGAAA ATGCTGGTGG AAACCTCTGA CCTTTCTACT 
TTCTGCTTTC ATCTCCGTAC CTTTGAGAAG ATGTTTGCTA CGACCCTGGA GGAACCAACC 
ATGCTTCGCT ACACCATTGC CTTCCCCCTG ATTTGTGCTC ATTTTGTCCA CTGCGTTCAT 
GAAATGTGCC CAGAGGAGTA CCCTCACCTG AAGAACCATG GTCTTCACCA CTGCAACTCC 
TTCCTGGAAG ATCTGGCCAA GCAGACCAGC AACTGTGTGC TTGAGATATG TGCGGAACAG 
CGGAACCTGA ATGAACAGCT TCTACCTAAG CACTGTGCCA CCACCATCAG CAAAGCCAAG 
AATAAGAAGT CCATGAAGCA GAGGCAGGCT CCCAGGAAAG GAGAGCCTGA GAGGGACAAG 
CCAGGAGCAG AGAGTCATCG GAAGAACCGC AGCCTTGTCA CTAACATGGA CAAACTACAT 
CTAAACTTGA CTGAATTGGC ACTGGCAATG AATCATGTGC ACAGTTTCTC TGTGTTTGAA 
CACACCATCT TCCCATCTGA GTATCTCAGC AGTCACCTGG AGGCCAGGCT TAACAGAGCC 
ATCGTGACTT TGGCTGGCTA CAATGCCACA ACCCAGGAGA TCCTTAGGCC TTCTGAGCTG 
CTGGCAGGAG TCAAAGCATA CATCAGTTTC ATCCAGTCAT TGGCACAGTT TTTGGGTGCA 
GATGCTTCCA GAATCGTTCG CAATGCCCTC CTGCAGCAGA CACAGCCACT GGACTCATCT 
GGCGAGCAGA CTGTCACCAC TCTCTACACA AACTGGTACC TAGAAAGTCT CCTTAGACAG 
GCGAGCAGTG GAGCCATTGT CCTCTCCCCA GCCATGCAGG CCTTCATCAG CCTTCCCCGA 
GATGGAGAGC AGAACTTCAG TGCTGAGGAG TTCTCGGACA TCTCTGAGAT GCGAGCCTTG 
GCTGAAATTC TTGGTCCCTA TGGCATGAAG TTCCTGAGTG AAAACCTGAT GTGGCATGTG 
ACCTCTCAGA TTGTGGAGCT GAAGAAGCTT GTGGTGGAAA ACATGGATAT TCTGGTCCAG 
ATCAGATCCA ACTTCAGCAA GCCAGAATTA ATGGCATCTC TGCTGCCCCA GCTGACAGGA 
GCTGAAAATG TGCTGAAACG AATGACCATC ATTGGGGTGA TTCTCAGTTT TAGGGCCATG 
GCCCAAGAGG GACTTCAGGA GGTTTTTTCT GCCCACTGCC CTTTTCTCAT GGGCCCCATT 
GAGTGCCTGA AGGAGTTTGT CACCCCTGAC ACAGACATTA AGGTCACCCT GAGTGTCTTT 
GAGCTGGCAT GTGCTGCAGG GGTGAGCTGT GACATTGACC CAGCCTTGGT AGCTGCCATT 
GCCAATCTGA AAGCTGATAA CTCATCCCCT GAAGAAGAGT ATAAGGTGGC ATGCCTACTC 
TTGATCTTTC TTGCTGTTTC CCTCCCACTC CTTGCCACTG ACCCGTCTTC CTTCTTCAGC 
ATTGAGAAAG ATGGCTACAA CAACAACATC CACTGTTTGA CCAAAGCCAT CATTCAGGTG 
TCTGCTGCCC TCTTCACTCT GTACAACAAG AACATTGAAA CGCACCTCAA AGAGTTTCTG 
GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG GGCCAGGAGA CTGACAAGCT CAAAACCAGA 
AATCGTGAAT CCATTTCTCT GCTCATGCGC TTGGTGGTGG AGGAGTCACC CTTCTTGACC 
CTGGACATGC TGGAGTCCTG TTTCCCTTAC GTTCTGCTAC GCAATGCCTA TCGGGAGGTT 
TCCCGGGCCT TCTACTTGAA CCGTCTGCCA GCCAGTTCCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520318.2
CDS	98..3502
Translation	MSLTSAYQLKLAEKLTILNDRGQGVLIRMYNIKKTCSDSKSKPPFLLEKSMESCLKYINKKFPNIDVRNSTQHLGPVHREKAEIIRFLTNYYQSFVDVMEFRDHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTSVILLLSRIEDRRILIGMYNCAHEMLHGHSDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHFLFVRRNQGAEQWRSAQLLSLISSPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQCQKLWKLCLEGSLYITLIREDVLQVHKVTEDLFSSLKGYSKRVADIKESKEHAITNSGQFHCQRRQFLRTAVKELETVLNDEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTKTKTPEDYADSSIAELLFLLEEIRALVRRHIKVIQQYHLQYLARFDVLVLSDIIQNLSVCPEEESVIMSSFVSILSSLNLKQVDNEEKFDFSGLRLDWFRLQAYTSVSKAPLHLHENPDLAKVMNLIIFHSQMLDSVEKMLVETSDLSTFCFHLRTFEKMFATTLEEPTMLRYTIAFPLICAHFVHCVHEMCPEEYPHLKNHGLHHCNSFLEDLAKQTSNCVLEICAEQRNLNEQLLPKHCATTISKAKNKKSMKQRQAPRKGEPERDKPGAESHRKNRSLVTNMDKLHLNLTELALAMNHVHSFSVFEHTIFPSEYLSSHLEARLNRAIVTLAGYNATTQEILRPSELLAGVKAYISFIQSLAQFLGADASRIVRNALLQQTQPLDSSGEQTVTTLYTNWYLESLLRQASSGAIVLSPAMQAFISLPRDGEQNFSAEEFSDISEMRALAEILGPYGMKFLSENLMWHVTSQIVELKKLVVENMDILVQIRSNFSKPELMASLLPQLTGAENVLKRMTIIGVILSFRAMAQEGLQEVFSAHCPFLMGPIECLKEFVTPDTDIKVTLSVFELACAAGVSCDIDPALVAAIANLKADNSSPEEEYKVACLLLIFLAVSLPLLATDPSSFFSIEKDGYNNNIHCLTKAIIQVSAALFTLYNKNIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESPFLTLDMLESCFPYVLLRNAYREVSRAFYLNRLPASSH

Target ORF information:

RefSeq Version	XM_006520255.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NCK associated protein 1 like (Nckap1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520255.3

ORF Insert Sequence:

ATGTCTTTGA CCTCTGCCTA TCAGCTTAAA TTAGCAGAGA AGCTCACGAT CCTGAATGAC 
CGAGGACAGG GAGTTCTCAT CCGCATGTAC AACATCAAGA AGACCTGTTC AGATTCAAAA 
TCCAAGCCAC CTTTTTTACT GGAAAAGTCC ATGGAATCAT GTCTCAAGTA CATCAACAAG 
AAGTTTCCCA ACATAGATGT CCGAAATAGC ACGCAACATT TAGGACCAGT GCATCGTGAA 
AAAGCTGAGA TAATTAGATT CCTCACTAAC TATTACCAGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTATATGA ACTTCTCAAC ACCATTGATG CCTGCCAGTG TCATTTTGAT 
ATTAATCTCA ACTTTGACTT CACTCGGAGC TACCTGGACT TAATTGTGAC GTACACCTCA 
GTCATTTTAC TCCTGTCACG GATTGAGGAC CGCAGGATAC TCATTGGCAT GTACAACTGT 
GCCCATGAGA TGCTGCATGG GCACAGTGAC CCCAGTTTTG CTCGCCTGGG CCAGATGGTA 
TTAGAGTATG ACCATCCTCT GAAGAAGCTG ACAGAAGAAT TTGGACCTCA CACAAAGGCT 
GTGAGTGGAG CCCTGCTCTC TCTGCACTTC CTCTTTGTTC GGAGGAACCA GGGAGCTGAG 
CAGTGGCGCA GCGCCCAGCT TCTAAGCCTC ATCAGCAGCC CCCCGGCCAT GATTAACCCT 
GCTAATTCAG ACACAATGGC CTGTGAGTAT CTGTCCGTGG AAGTGATGGA GCGATGGATC 
ATAATTGGGT TTCTTCTTTG CCATGGGTGC CTCAACTCCA ACAGCCAGTG CCAGAAGCTG 
TGGAAGCTGT GTCTGGAGGG TTCCCTGTAC ATCACGCTCA TCCGGGAAGA TGTGCTGCAA 
GTGCACAAGG TCACCGAGGA CCTGTTCAGC AGTTTGAAAG GGTACAGTAA GCGAGTGGCA 
GACATCAAGG AGAGCAAGGA ACACGCCATT ACAAACAGTG GCCAGTTTCA CTGTCAACGC 
CGGCAGTTTC TGCGGACAGC TGTGAAGGAG TTGGAGACTG TGTTAAATGA TGAACCAGGC 
CTGCTTGGTC CTAAGGCCCT TTTTGCTTTC ATGGCCCTGT CTTTCATTCG TGATGAGGTC 
ACTTGGCTGG TTCGCCATAC AGAAAATGTC ACCAAGACTA AGACACCCGA AGACTATGCT 
GACTCGAGCA TTGCAGAGCT TCTGTTCCTG TTGGAGGAGA TCAGGGCTCT GGTCAGAAGA 
CACATCAAAG TTATTCAGCA GTACCACCTC CAGTACCTGG CCAGATTTGA TGTCCTTGTG 
CTTAGTGACA TCATTCAGAA TCTGTCCGTG TGTCCAGAGG AGGAGTCTGT CATTATGTCC 
TCATTTGTTA GTATACTGTC CTCTCTGAAT CTCAAGCAAG TTGATAATGA AGAGAAATTT 
GACTTTTCTG GATTAAGGCT GGATTGGTTT CGCCTACAGG CATATACCAG CGTGTCGAAG 
GCACCTTTGC ACCTGCATGA GAACCCTGAC CTAGCCAAGG TCATGAACCT CATCATCTTT 
CACTCCCAGA TGTTGGACTC AGTAGAGAAA ATGCTGGTGG AAACCTCTGA CCTTTCTACT 
TTCTGCTTTC ATCTCCGTAC CTTTGAGAAG ATGTTTGCTA CGACCCTGGA GGAACCAACC 
ATGCTTCGCT ACACCATTGC CTTCCCCCTG ATTTGTGCTC ATTTTGTCCA CTGCGTTCAT 
GAAATGTGCC CAGAGGAGTA CCCTCACCTG AAGAACCATG GTCTTCACCA CTGCAACTCC 
TTCCTGGAAG ATCTGGCCAA GCAGACCAGC AACTGTGTGC TTGAGATATG TGCGGAACAG 
CGGAACCTGA ATGAACAGCT TCTACCTAAG CACTGTGCCA CCACCATCAG CAAAGCCAAG 
AATAAGAAGT CCATGAAGCA GAGGCAGGCT CCCAGGAAAG GAGAGCCTGA GAGGGACAAG 
CCAGGAGCAG AGAGTCATCG GAAGAACCGC AGCCTTGTCA CTAACATGGA CAAACTACAT 
CTAAACTTGA CTGAATTGGC ACTGGCAATG AATCATGTGC ACAGTTTCTC TGTGTTTGAA 
CACACCATCT TCCCATCTGA GTATCTCAGC AGTCACCTGG AGGCCAGGCT TAACAGAGCC 
ATCGTGACTT TGGCTGGCTA CAATGCCACA ACCCAGGAGA TCCTTAGGCC TTCTGAGCTG 
CTGGCAGGAG TCAAAGCATA CATCAGTTTC ATCCAGTCAT TGGCACAGTT TTTGGGTGCA 
GATGCTTCCA GAATCGTTCG CAATGCCCTC CTGCAGCAGA CACAGCCACT GGACTCATCT 
GGCGAGCAGA CTGTCACCAC TCTCTACACA AACTGGTACC TAGAAAGTCT CCTTAGACAG 
GCGAGCAGTG GAGCCATTGT CCTCTCCCCA GCCATGCAGG CCTTCATCAG CCTTCCCCGA 
GATGGAGAGC AGAACTTCAG TGCTGAGGAG TTCTCGGACA TCTCTGAGAT GCGAGCCTTG 
GCTGAAATTC TTGGTCCCTA TGGCATGAAG TTCCTGAGTG AAAACCTGAT GTGGCATGTG 
ACCTCTCAGA TTGTGGAGCT GAAGAAGCTT GTGGTGGAAA ACATGGATAT TCTGGTCCAG 
ATCAGATCCA ACTTCAGCAA GCCAGAATTA ATGGCATCTC TGCTGCCCCA GCTGACAGGA 
GCTGAAAATG TGCTGAAACG AATGACCATC ATTGGGGTGA TTCTCAGTTT TAGGGCCATG 
GCCCAAGAGG GACTTCAGGA GGTTTTTTCT GCCCACTGCC CTTTTCTCAT GGGCCCCATT 
GAGTGCCTGA AGGAGTTTGT CACCCCTGAC ACAGACATTA AGGTCACCCT GAGTGTCTTT 
GAGCTGGCAT GTGCTGCAGG GGTGAGCTGT GACATTGACC CAGCCTTGGT AGCTGCCATT 
GCCAATCTGA AAGCTGATAA CTCATCCCCT GAAGAAGAGT ATAAGGTGGC ATGCCTACTC 
TTGATCTTTC TTGCTGTTTC CCTCCCACTC CTTGCCACTG ACCCGTCTTC CTTCTTCAGC 
ATTGAGAAAG ATGGCTACAA CAACAACATC CACTGTTTGA CCAAAGCCAT CATTCAGGTG 
TCTGCTGCCC TCTTCACTCT GTACAACAAG AACATTGAAA CGCACCTCAA AGAGTTTCTG 
GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG GGCCAGGAGA CTGACAAGCT CAAAACCAGA 
AATCGTGAAT CCATTTCTCT GCTCATGCGC TTGGTGGTGG AGGAGTCACC CTTCTTGACC 
CTGGACATGC TGGAGTCCTG TTTCCCTTAC GTTCTGCTAC GCAATGCCTA TCGGGAGGTT 
TCCCGGGCCT TCTACTTGAA CCGTCTGCCA GCCAGTTCCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A functional genomics predictive network model identifies regulators of inflammatory bowel disease.
Nature genetics49(10)1437-1449(2017 Oct)
Peters LA,Perrigoue J,Mortha A,Iuga A,Song WM,Neiman EM,Llewellyn SR,Di Narzo A,Kidd BA,Telesco SE,Zhao Y,Stojmirovic A,Sendecki J,Shameer K,Miotto R,Losic B,Shah H,Lee E,Wang M,Faith JJ,Kasarskis A,Brodmerkel C,Curran M,Das A,Friedman JR,Fukui Y,Humphrey MB,Iritani BM,Sibinga N,Tarrant TK,Argmann C,Hao K,Roussos P,Zhu J,Zhang B,Dobrin R,Mayer LF,Schadt EE

Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of?leukocytes.
Nature cell biology18(11)1253-1259(2016 Nov)
Leithner A,Eichner A,M?ller J,Reversat A,Brown M,Schwarz J,Merrin J,de Gorter DJ,Schur F,Bayerl J,de Vries I,Wieser S,Hauschild R,Lai FP,Moser M,Kerjaschki D,Rottner K,Small JV,Stradal TE,Sixt M

Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments.
Nature communications710997(2016 Mar)
Thiam HR,Vargas P,Carpi N,Crespo CL,Raab M,Terriac E,King MC,Jacobelli J,Alberts AS,Stradal T,Lennon-Dumenil AM,Piel M

Hematopoietic protein-1 regulates the actin membrane skeleton and membrane stability in murine erythrocytes.
PloS one8(2)e54902(2013)
Chan MM,Wooden JM,Tsang M,Gilligan DM,Hirenallur-S DK,Finney GL,Rynes E,Maccoss M,Ramirez JA,Park H,Iritani BM

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Nckap1l cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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