Pelo cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pelo
Entrez Gene ID	105083
Full Name	pelota mRNA surveillance and ribosome rescue factor
Synonyms	AA409897,AI852218
General protein information	Preferred Names pelota mRNA surveillance and ribosome rescue factor Names protein pelota homolog pelota homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 D2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_134058.3	NP_598819.2	protein pelota homolog

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pelo	NP_957430.1
Bos taurus (cattle)	PELO	NP_001029738.1
Homo sapiens (human)	PELO	NP_057030.3
Gallus gallus (chicken)	PELO	NP_001026763.1
Drosophila melanogaster (fruit fly)	pelo	NP_476982.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008269	XP_317201.4
Schizosaccharomyces pombe (fission yeast)	SPCC18B5.06	NP_587936.1
Canis lupus familiaris (dog)	PELO	XP_536477.3
Mus musculus (house mouse)	Pelo	NP_598819.2
Rattus norvegicus (Norway rat)	Pelo	NP_001007635.1
Caenorhabditis elegans (roundworm)	R74.6	NP_497842.1

Gene Ontology
Bibliography

Related articles in PubMed

Pelota Regulates Epidermal Differentiation by Modulating BMP and PI3K/AKT Signaling?Pathways.
Elkenani M, Nyamsuren G, Raju P, Liakath-Ali K, Hamdaoui A, Kata A, Dressel R, Klonisch T, Watt FM, Engel W, Thliveris JA, Krishna Pantakani DV, Adham IM
The Journal of investigative dermatology136(8)1664-71(2016 Aug)

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

Pelota mediates gonocyte maturation and maintenance of spermatogonial stem cells in mouse testes.
Raju P, Nyamsuren G, Elkenani M, Kata A, Tsagaan E, Engel W, Adham IM
Reproduction (Cambridge, England)149(3)213-21(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

increased activity of the PI3K/AKT signaling pathway in Pelo-deficient skin might conflict with the dephosphorylation of profilaggrin and thereby affect its proper processing into filaggrin monomers and ultimately the epidermal differentiation
Title: Pelota Regulates Epidermal Differentiation by Modulating BMP and PI3K/AKT Signaling?Pathways.

These results suggest that PELO negatively regulates the PI3K/AKT pathway and that the enhanced activity of PI3K/AKT and subsequent FOXO1 inhibition are responsible for the impaired development of spermatogonial stem cells in mutant testes.
Title: Pelota mediates gonocyte maturation and maintenance of spermatogonial stem cells in mouse testes.

these results not only define GTPBP2 as a ribosome rescue factor but also unmask the disease potential of mutations in nuclear-encoded tRNA genes.
Title: RNA function. Ribosome stalling induced by mutation of a CNS-specific tRNA causes neurodegeneration.

Results indicate that PELO plays an important role in the establishment of pluripotency and differentiation of ESCs into ExEn lineage through activation of BMP signaling.
Title: Pelota regulates the development of extraembryonic endoderm through activation of bone morphogenetic protein (BMP) signaling.

Pelo is required for the maintenance of genomic stability
Title: Disruption of the pelota gene causes early embryonic lethality and defects in cell cycle progression.

The following Pelo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pelo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10487	NM_134058.3 Latest version!	Mus musculus pelota mRNA surveillance and ribosome rescue factor (Pelo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pelo ( NM_134058.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598819.2 Mus musculus pelota mRNA surveillance and ribosome rescue factor (Pelo), mRNA.

Price & Availability↑

CloneID	OMu10487
Clone ID Related Accession (Same CDS sequence)	NM_134058.3
Accession Version	NM_134058.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	protein pelota homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC175538.1, BC050209.1, BB767039.1 and AI854576.1. On Jan 15, 2008 this sequence version replaced NM_134058.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050209.1, AK170635.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCTCG TGAGGAAGGA TATCGAGAAG GACAATGCGG GCCAGGTCAC CCTCGTCCCC 
GAGGAGCCCG AGGACATGTG GCACACCTTC AATTTAGTGC AGGTGGGCGA CAGCCTGCGC 
GCCTCCACCA TCCGCAAGGT CCAGACCGAG TCCTCCACAG GCAGCGTGGG GAGCAACCGT 
GTCCGCACCA CCCTCACCCT TTGCGTGGAG GCCATCGACT TCGACTCCCA GGCCTGCCAG 
CTGCGGGTGA AGGGGACCAA TATCCAAGAG AATGAGTATG TCAAGATGGG GGCTTACCAC 
ACCATCGAAC TGGAGCCCAA CCGCCAGTTC ACCCTGGCCA AGAAACAGTG GGACAGTGTG 
GTTCTGGAAC GCATCGAGCA GGCTTGCGAC CCGGCGTGGA GCGCTGATGT GGCAGCTGTG 
GTCATGCAGG AGGGCCTGGC CCACGTCTGC TTAGTCACGC CCAGCATGAC CCTTACCCGG 
GCCAAGGTGG AGGTGAACAT CCCCAGGAAA CGGAAAGGCA ACTGCTCCCA GCATGACCGG 
GCCCTGGAGC GGTTCTATGA ACAGGTGGTC CAGGCCATCC AGCGCCACAT AAACTTCGAG 
GTTGTAAAGT GCGTTCTGGT GGCCAGCCCT GGATTCGTGC GAGAGCAGTT CTGCGATTAC 
ATGTTTCAGC AAGCTGTGAA GACGGACAAT AAAGTGCTCC TGGAAAACCG CTCCAAATTC 
CTTCAGGTGC ACGCCTCCTC TGGACACAAG TACTCCCTGA AAGAGGCCCT CTGCGACCCT 
ACTGTAGCGA GCCGCCTTTC AGACACAAAG GCAGCGGGCG AAGTAAAAGC CTTGGATGAC 
TTCTACAAAA TGTTACAGCA CGAGCCCGAC CGAGCGTTCT ATGGACTCAA GCAGGTGGAG 
AGGGCCAACG AAGCCTTGGC CATAGACACT TTGCTCATCA GTGACGAGCT CTTCCGGCAT 
CAGGATGTGG CCACCCGGAG CCGGTATGTC AGGCTAGTGG ACAGTGTGAA GGAGAATGCG 
GGCACCGTAA GGATATTCTC TAGTCTCCAT GTGTCGGGGG AACAGCTGGG CCAGCTGACG 
GGAGTAGCCG CCATTCTCCG CTTCCCAGTG CCAGAACTTT CTGACCAAGA GGATGATTCA 
AGTTCTGAAG AGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598819.2
CDS	240..1397
Translation	MKLVRKDIEKDNAGQVTLVPEEPEDMWHTFNLVQVGDSLRASTIRKVQTESSTGSVGSNRVRTTLTLCVEAIDFDSQACQLRVKGTNIQENEYVKMGAYHTIELEPNRQFTLAKKQWDSVVLERIEQACDPAWSADVAAVVMQEGLAHVCLVTPSMTLTRAKVEVNIPRKRKGNCSQHDRALERFYEQVVQAIQRHINFEVVKCVLVASPGFVREQFCDYMFQQAVKTDNKVLLENRSKFLQVHASSGHKYSLKEALCDPTVASRLSDTKAAGEVKALDDFYKMLQHEPDRAFYGLKQVERANEALAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLHVSGEQLGQLTGVAAILRFPVPELSDQEDDSSSEED

Target ORF information:

RefSeq Version	NM_134058.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pelota mRNA surveillance and ribosome rescue factor (Pelo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134058.3

ORF Insert Sequence:

ATGAAGCTCG TGAGGAAGGA TATCGAGAAG GACAATGCGG GCCAGGTCAC CCTCGTCCCC 
GAGGAGCCCG AGGACATGTG GCACACCTTC AATTTAGTGC AGGTGGGCGA CAGCCTGCGC 
GCCTCCACCA TCCGCAAGGT CCAGACCGAG TCCTCCACAG GCAGCGTGGG GAGCAACCGT 
GTCCGCACCA CCCTCACCCT TTGCGTGGAG GCCATCGACT TCGACTCCCA GGCCTGCCAG 
CTGCGGGTGA AGGGGACCAA TATCCAAGAG AATGAGTATG TCAAGATGGG GGCTTACCAC 
ACCATCGAAC TGGAGCCCAA CCGCCAGTTC ACCCTGGCCA AGAAACAGTG GGACAGTGTG 
GTTCTGGAAC GCATCGAGCA GGCTTGCGAC CCGGCGTGGA GCGCTGATGT GGCAGCTGTG 
GTCATGCAGG AGGGCCTGGC CCACGTCTGC TTAGTCACGC CCAGCATGAC CCTTACCCGG 
GCCAAGGTGG AGGTGAACAT CCCCAGGAAA CGGAAAGGCA ACTGCTCCCA GCATGACCGG 
GCCCTGGAGC GGTTCTATGA ACAGGTGGTC CAGGCCATCC AGCGCCACAT AAACTTCGAG 
GTTGTAAAGT GCGTTCTGGT GGCCAGCCCT GGATTCGTGC GAGAGCAGTT CTGCGATTAC 
ATGTTTCAGC AAGCTGTGAA GACGGACAAT AAAGTGCTCC TGGAAAACCG CTCCAAATTC 
CTTCAGGTGC ACGCCTCCTC TGGACACAAG TACTCCCTGA AAGAGGCCCT CTGCGACCCT 
ACTGTAGCGA GCCGCCTTTC AGACACAAAG GCAGCGGGCG AAGTAAAAGC CTTGGATGAC 
TTCTACAAAA TGTTACAGCA CGAGCCCGAC CGAGCGTTCT ATGGACTCAA GCAGGTGGAG 
AGGGCCAACG AAGCCTTGGC CATAGACACT TTGCTCATCA GTGACGAGCT CTTCCGGCAT 
CAGGATGTGG CCACCCGGAG CCGGTATGTC AGGCTAGTGG ACAGTGTGAA GGAGAATGCG 
GGCACCGTAA GGATATTCTC TAGTCTCCAT GTGTCGGGGG AACAGCTGGG CCAGCTGACG 
GGAGTAGCCG CCATTCTCCG CTTCCCAGTG CCAGAACTTT CTGACCAAGA GGATGATTCA 
AGTTCTGAAG AGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pelota Regulates Epidermal Differentiation by Modulating BMP and PI3K/AKT Signaling?Pathways.
The Journal of investigative dermatology136(8)1664-71(2016 Aug)
Elkenani M,Nyamsuren G,Raju P,Liakath-Ali K,Hamdaoui A,Kata A,Dressel R,Klonisch T,Watt FM,Engel W,Thliveris JA,Krishna Pantakani DV,Adham IM

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J

Pelota mediates gonocyte maturation and maintenance of spermatogonial stem cells in mouse testes.
Reproduction (Cambridge, England)149(3)213-21(2015 Mar)
Raju P,Nyamsuren G,Elkenani M,Kata A,Tsagaan E,Engel W,Adham IM

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Pelo cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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