Tdp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tdp1
Entrez Gene ID	104884
Full Name	tyrosyl-DNA phosphodiesterase 1
Synonyms	2810481F14Rik,4921509N21Rik,AI838772,AW493413,E430034L06Rik,MLZ-501,SCAN1
General protein information	Preferred Names tyrosyl-DNA phosphodiesterase 1 Names tyrosyl-DNA phosphodiesterase 1 protein expressed in male leptotene and zygotene spermatocytes 501 tyr-DNA phosphodiesterase 1 tyrosyl-DNA phodphodiesterase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 E

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515363.3	XP_006515426.1	tyrosyl-DNA phosphodiesterase 1 isoform X1
XM_006515364.3	XP_006515427.1	tyrosyl-DNA phosphodiesterase 1 isoform X2
XM_030246506.1	XP_030102366.1	tyrosyl-DNA phosphodiesterase 1 isoform X1
XM_006515364.4	XP_006515427.1	tyrosyl-DNA phosphodiesterase 1 isoform X2
NM_028354.4	NP_082630.2	tyrosyl-DNA phosphodiesterase 1
XM_030246507.1	XP_030102367.1	tyrosyl-DNA phosphodiesterase 1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	TDP1	NP_001008744.1
Danio rerio (zebrafish)	tdp1	XP_700174.2
Xenopus tropicalis (tropical clawed frog)	tdp1	NP_001039242.1
	TDP1	XP_001089545.1
Canis lupus familiaris (dog)	TDP1	XP_547950.2
Bos taurus (cattle)	TDP1	NP_001180084.1
Gallus gallus (chicken)	TDP1	XP_004941916.1
Caenorhabditis elegans (roundworm)	F52C12.1	NP_500149.1
Oryza sativa (rice)	Os07g0530100	NP_001059844.1
Pan troglodytes (chimpanzee)	TDP1	NP_001233557.1
Mus musculus (house mouse)	Tdp1	NP_082630.2
Rattus norvegicus (Norway rat)	Tdp1	NP_001026827.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010577	XP_558110.3
Arabidopsis thaliana (thale cress)	TDP1	NP_197021.2
Drosophila melanogaster (fruit fly)	gkt	NP_523465.2

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial protein-linked DNA breaks perturb mitochondrial gene transcription and trigger free radical-induced DNA damage.
Chiang SC, Meagher M, Kassouf N, Hafezparast M, McKinnon PJ, Haywood R, El-Khamisy SF
Science advances3(4)e1602506(2017 Apr)

Aberrant topoisomerase-1 DNA lesions are pathogenic in neurodegenerative genome instability syndromes.
Katyal S, Lee Y, Nitiss KC, Downing SM, Li Y, Shimada M, Zhao J, Russell HR, Petrini JH, Nitiss JL, McKinnon PJ
Nature neuroscience17(6)813-21(2014 Jun)

R-loops in proliferating cells but not in the brain: implications for AOA2 and other autosomal recessive ataxias.
Yeo AJ, Becherel OJ, Luff JE, Cullen JK, Wongsurawat T, Jenjaroenpun P, Jenjaroenpoon P, Kuznetsov VA, McKinnon PJ, Lavin MF
PloS one9(3)e90219(2014)

Pol ? associated complex and base excision repair factors in mouse fibroblasts.
Prasad R, Williams JG, Hou EW, Wilson SH
Nucleic acids research40(22)11571-82(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings provide evidence for TDP1 as a novel mitochondrial enzyme.
Title: Role of tyrosyl-DNA phosphodiesterase (TDP1) in mitochondria.

The interaction with Lig3alpha is promoted by serine 81 that is located within a putative S/TQ site in the N-terminus domain of TDP1.
Title: TDP1 serine 81 promotes interaction with DNA ligase IIIalpha and facilitates cell survival following DNA damage.

Tdp1(-/-) fibroblasts exhibited deficiencies in processing 3'-phosphotyrosyl single-strand breaks and 3'-phosphoglycolate double-strand breaks, but not 3'-phosphoglycolate single-strand breaks.
Title: In vitro complementation of Tdp1 deficiency indicates a stabilized enzyme-DNA adduct from tyrosyl but not glycolate lesions as a consequence of the SCAN1 mutation.

Data indicate that TDP1 is required for neural homeostasis and reveal a widespread requisite for TDP1 function in response to acutely elevated levels of DNA topoisomerase I (Top1)-associated DNA strand breaks.
Title: TDP1 facilitates chromosomal single-strand break repair in neurons and is neuroprotective in vivo.

This study provides a direct demonstration that Tdp1 repairs Topo I covalent lesions in vivo and suggests that spinocerebellar ataxia with axonal neuropathy (SCAN1) arises from the recessive neomorphic mutation H493R.
Title: Spinocerebellar ataxia with axonal neuropathy: consequence of a Tdp1 recessive neomorphic mutation?

The following Tdp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tdp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15409	NM_028354.4 Latest version!	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu47508	XM_006515363.3 Latest version!	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu47508	XM_030246506.1 Latest version!	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu47509	XM_006515364.4 Latest version!	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu85979	XM_030246507.1 Latest version!	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu47509	XM_006515364.3	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tdp1 ( NM_028354.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082630.2 Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), mRNA.

Price & Availability↑

CloneID	OMu15409
Clone ID Related Accession (Same CDS sequence)	NM_028354.4
Accession Version	NM_028354.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	tyrosyl-DNA phosphodiesterase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ151851.1 and BC065162.1. On or before Mar 1, 2008 this sequence version replaced XM_001473901.1, NM_028354.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.172474.1, AK014855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCAAG AAAGCAGCTA CGGGAAGTGG ACAATATCCA GTAGTGACGA AAGCGAGGAT 
GAGAAGCCCA AACCTGATAA GCCGTCAGCA TCCTCTCACC CCCAGGCTGG GCAGGGAGTG 
TCGAAGGAGC TCATATACAC CTGCTCAGAG GCCCGCAAGG TTGCACACAA GAGACAGATC 
TCACCTGTGA AGTTCAACGA CGCAGACTCG GTGCTACCCC ACAAGAAGCA GAAGAGTGAC 
TCCCCGGAAG GTCTCGGCTG GTGTCTCTCC AGCAGCGATG ATGACCAGCA GCCAGATGTG 
ACACAGCAGG AACAGCCTAA GAGAGTGCTG CCTCAAGAGA AAAAACATGT CTCCTCACCC 
GATGTGACTA CTGCCCAGAA AGTTGTGGAT AGAAGCCCTC CTGCCAGCCT TAGGCCCCAA 
AGGGCAGACG ATGAGTACGA GACCTCAGGG GAAGGCCAGG ACATCTGGGA CATGCTGGAT 
AAGGGGAACC CCTTCCAGTT TTATCTCACT AGAGTGTCGG GGATTAAGGC AAAGTACAAC 
TCCAAAGCCC TCCACATCAA GGATATTTTA TCCCCTCTGT TTGGGACACT TGTCTCTTCA 
GCTCAGTTTA ACTACTGCTT TGACGTGGAC TGGCTCATAA AGCAATATCC ACCGGAATTC 
AGGAAGAACC CGATCCTGCT TGTGCACGGT GATAAGAGGG AAGCCAAGGC AGACTTACAT 
GCCCAGGCGA AGCCCTATGC GAACATTTCC CTCTGCCAGG CAAAGTTGGA TATTGCATTT 
GGAACACACC ACACGAAAAT GATGCTCTTG CTCTATGAAG AAGGCCTCCG AGTTGTCATC 
CACACCTCCA ACCTCATCCG GGAAGACTGG CACCAGAAAA CCCAAGGAAT ATGGTTGAGC 
CCTTTGTACC CAAGAATTGA CCAGGGAAGC CACACAGCTG GAGAGTCAAG CACCCGTTTC 
AAAGCTGACC TCACCAGTTA TCTAACGGCG TATAACGCTC CCCCTCTGCA GGAGTGGATA 
GACATCATCC AAGAGCATGA CCTCTCTGAG ACCAATGTTT ACCTCATTGG TTCAACCCCT 
GGACGCTTTC AAGGAAGTCA CAGAGATAAC TGGGGACATT TTAGGCTTAG AAAGCTTCTG 
CAAGCGCATG CCCCGTCCAC ACCCAAGGGC GAGTGCTGGC CTATCGTGGG TCAGTTCTCA 
AGCATCGGCT CCCTGGGGCC CGATGAATCC AAGTGGCTGT GCTCGGAGTT TAAAGACAGC 
TTGCTGGCAC TGAGAGAGGA AGGCCGACCT CCGGGCAAGA GTGCAGTTCC TCTTCATCTG 
ATCTATCCCT CTGTGGAAAA TGTGCGGACC AGCTTAGAAG GATACCCTGC TGGGGGCTCT 
CTTCCCTATA GCATCCAGAC GGCTGAAAAG CAGAGGTGGC TGCACTCCTA CTTCCACAAG 
TGGTCAGCAG AGACATCCGG CCGAAGTAAT GCCATGCCAC ACATTAAGAC GTACATGAGG 
CCCTCGCCAG ACTTCAGTAA ACTGGCTTGG TTCCTGGTCA CAAGTGCGAA TCTGTCCAAG 
GCTGCCTGGG GGGCACTGGA GAAGAACGGC ACCCAGCTGA TGATACGCTC CTACGAGCTC 
GGGGTCCTCT TCCTCCCGTC TGCGTTTGGC CTGGACACCT TCAAAGTGAA ACAGAAATTC 
TTCTCCAGCA GCTGTGAGCC GACGGCCTCC TTTCCTGTGC CCTATGACCT GCCCCCAGAG 
CTGTACGGGA GTAAAGACCG GCCTTGGATT TGGAACATTC CTTATGTCAA AGCACCTGAC 
ACGCACGGCA ACATGTGGGT GCCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082630.2
CDS	46..1875
Translation	MSQESSYGKWTISSSDESEDEKPKPDKPSASSHPQAGQGVSKELIYTCSEARKVAHKRQISPVKFNDADSVLPHKKQKSDSPEGLGWCLSSSDDDQQPDVTQQEQPKRVLPQEKKHVSSPDVTTAQKVVDRSPPASLRPQRADDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGIKAKYNSKALHIKDILSPLFGTLVSSAQFNYCFDVDWLIKQYPPEFRKNPILLVHGDKREAKADLHAQAKPYANISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIREDWHQKTQGIWLSPLYPRIDQGSHTAGESSTRFKADLTSYLTAYNAPPLQEWIDIIQEHDLSETNVYLIGSTPGRFQGSHRDNWGHFRLRKLLQAHAPSTPKGECWPIVGQFSSIGSLGPDESKWLCSEFKDSLLALREEGRPPGKSAVPLHLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQRWLHSYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFGLDTFKVKQKFFSSSCEPTASFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPS

Target ORF information:

RefSeq Version	NM_028354.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028354.4

ORF Insert Sequence:

ATGTCCCAAG AAAGCAGCTA CGGGAAGTGG ACAATATCCA GTAGTGACGA AAGCGAGGAT 
GAGAAGCCCA AACCTGATAA GCCGTCAGCA TCCTCTCACC CCCAGGCTGG GCAGGGAGTG 
TCGAAGGAGC TCATATACAC CTGCTCAGAG GCCCGCAAGG TTGCACACAA GAGACAGATC 
TCACCTGTGA AGTTCAACGA CGCAGACTCG GTGCTACCCC ACAAGAAGCA GAAGAGTGAC 
TCCCCGGAAG GTCTCGGCTG GTGTCTCTCC AGCAGCGATG ATGACCAGCA GCCAGATGTG 
ACACAGCAGG AACAGCCTAA GAGAGTGCTG CCTCAAGAGA AAAAACATGT CTCCTCACCC 
GATGTGACTA CTGCCCAGAA AGTTGTGGAT AGAAGCCCTC CTGCCAGCCT TAGGCCCCAA 
AGGGCAGACG ATGAGTACGA GACCTCAGGG GAAGGCCAGG ACATCTGGGA CATGCTGGAT 
AAGGGGAACC CCTTCCAGTT TTATCTCACT AGAGTGTCGG GGATTAAGGC AAAGTACAAC 
TCCAAAGCCC TCCACATCAA GGATATTTTA TCCCCTCTGT TTGGGACACT TGTCTCTTCA 
GCTCAGTTTA ACTACTGCTT TGACGTGGAC TGGCTCATAA AGCAATATCC ACCGGAATTC 
AGGAAGAACC CGATCCTGCT TGTGCACGGT GATAAGAGGG AAGCCAAGGC AGACTTACAT 
GCCCAGGCGA AGCCCTATGC GAACATTTCC CTCTGCCAGG CAAAGTTGGA TATTGCATTT 
GGAACACACC ACACGAAAAT GATGCTCTTG CTCTATGAAG AAGGCCTCCG AGTTGTCATC 
CACACCTCCA ACCTCATCCG GGAAGACTGG CACCAGAAAA CCCAAGGAAT ATGGTTGAGC 
CCTTTGTACC CAAGAATTGA CCAGGGAAGC CACACAGCTG GAGAGTCAAG CACCCGTTTC 
AAAGCTGACC TCACCAGTTA TCTAACGGCG TATAACGCTC CCCCTCTGCA GGAGTGGATA 
GACATCATCC AAGAGCATGA CCTCTCTGAG ACCAATGTTT ACCTCATTGG TTCAACCCCT 
GGACGCTTTC AAGGAAGTCA CAGAGATAAC TGGGGACATT TTAGGCTTAG AAAGCTTCTG 
CAAGCGCATG CCCCGTCCAC ACCCAAGGGC GAGTGCTGGC CTATCGTGGG TCAGTTCTCA 
AGCATCGGCT CCCTGGGGCC CGATGAATCC AAGTGGCTGT GCTCGGAGTT TAAAGACAGC 
TTGCTGGCAC TGAGAGAGGA AGGCCGACCT CCGGGCAAGA GTGCAGTTCC TCTTCATCTG 
ATCTATCCCT CTGTGGAAAA TGTGCGGACC AGCTTAGAAG GATACCCTGC TGGGGGCTCT 
CTTCCCTATA GCATCCAGAC GGCTGAAAAG CAGAGGTGGC TGCACTCCTA CTTCCACAAG 
TGGTCAGCAG AGACATCCGG CCGAAGTAAT GCCATGCCAC ACATTAAGAC GTACATGAGG 
CCCTCGCCAG ACTTCAGTAA ACTGGCTTGG TTCCTGGTCA CAAGTGCGAA TCTGTCCAAG 
GCTGCCTGGG GGGCACTGGA GAAGAACGGC ACCCAGCTGA TGATACGCTC CTACGAGCTC 
GGGGTCCTCT TCCTCCCGTC TGCGTTTGGC CTGGACACCT TCAAAGTGAA ACAGAAATTC 
TTCTCCAGCA GCTGTGAGCC GACGGCCTCC TTTCCTGTGC CCTATGACCT GCCCCCAGAG 
CTGTACGGGA GTAAAGACCG GCCTTGGATT TGGAACATTC CTTATGTCAA AGCACCTGAC 
ACGCACGGCA ACATGTGGGT GCCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tdp1 ( XM_006515363.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515426.1 Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47508
Clone ID Related Accession (Same CDS sequence)	XM_006515363.3 , XM_030246506.1
Accession Version	XM_006515363.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tyrosyl-DNA phosphodiesterase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTA TAATGTCCCA AGAAAGCAGC TACGGGAAGT GGACAATATC CAGTAGTGAC 
GAAAGCGAGG ATGAGAAGCC CAAACCTGAT AAGCCGTCAG CATCCTCTCA CCCCCAGGCT 
GGGCAGGGAG TGTCGAAGGA GCTCATATAC ACCTGCTCAG AGGCCCGCAA GGTTGCACAC 
AAGAGACAGA TCTCACCTGT GAAGTTCAAC GACGCAGACT CGGTGCTACC CCACAAGAAG 
CAGAAGAGTG ACTCCCCGGA AGGTCTCGGC TGGTGTCTCT CCAGCAGCGA TGATGACCAG 
CAGCCAGATG TGACACAGCA GGAACAGCCT AAGAGAGTGC TGCCTCAAGA GAAAAAACAT 
GTCTCCTCAC CCGATGTGAC TACTGCCCAG AAAGTTGTGG ATAGAAGCCC TCCTGCCAGC 
CTTAGGCCCC AAAGGGCAGA CGATGAGTAC GAGACCTCAG GGGAAGGCCA GGACATCTGG 
GACATGCTGG ATAAGGGGAA CCCCTTCCAG TTTTATCTCA CTAGAGTGTC GGGGATTAAG 
GCAAAGTACA ACTCCAAAGC CCTCCACATC AAGGATATTT TATCCCCTCT GTTTGGGACA 
CTTGTCTCTT CAGCTCAGTT TAACTACTGC TTTGACGTGG ACTGGCTCAT AAAGCAATAT 
CCACCGGAAT TCAGGAAGAA CCCGATCCTG CTTGTGCACG GTGATAAGAG GGAAGCCAAG 
GCAGACTTAC ATGCCCAGGC GAAGCCCTAT GCGAACATTT CCCTCTGCCA GGCAAAGTTG 
GATATTGCAT TTGGAACACA CCACACGAAA ATGATGCTCT TGCTCTATGA AGAAGGCCTC 
CGAGTTGTCA TCCACACCTC CAACCTCATC CGGGAAGACT GGCACCAGAA AACCCAAGGA 
ATATGGTTGA GCCCTTTGTA CCCAAGAATT GACCAGGGAA GCCACACAGC TGGAGAGTCA 
AGCACCCGTT TCAAAGCTGA CCTCACCAGT TATCTAACGG CGTATAACGC TCCCCCTCTG 
CAGGAGTGGA TAGACATCAT CCAAGAGCAT GACCTCTCTG AGACCAATGT TTACCTCATT 
GGTTCAACCC CTGGACGCTT TCAAGGAAGT CACAGAGATA ACTGGGGACA TTTTAGGCTT 
AGAAAGCTTC TGCAAGCGCA TGCCCCGTCC ACACCCAAGG GCGAGTGCTG GCCTATCGTG 
GGTCAGTTCT CAAGCATCGG CTCCCTGGGG CCCGATGAAT CCAAGTGGCT GTGCTCGGAG 
TTTAAAGACA GCTTGCTGGC ACTGAGAGAG GAAGGCCGAC CTCCGGGCAA GAGTGCAGTT 
CCTCTTCATC TGATCTATCC CTCTGTGGAA AATGTGCGGA CCAGCTTAGA AGGATACCCT 
GCTGGGGGCT CTCTTCCCTA TAGCATCCAG ACGGCTGAAA AGCAGAGGTG GCTGCACTCC 
TACTTCCACA AGTGGTCAGC AGAGACATCC GGCCGAAGTA ATGCCATGCC ACACATTAAG 
ACGTACATGA GGCCCTCGCC AGACTTCAGT AAACTGGCTT GGTTCCTGGT CACAAGTGCG 
AATCTGTCCA AGGCTGCCTG GGGGGCACTG GAGAAGAACG GCACCCAGCT GATGATACGC 
TCCTACGAGC TCGGGGTCCT CTTCCTCCCG TCTGCGTTTT CCTCACCTGT CCTCCTCCAC 
CCCAGAGGGT TTCAGGATGG AACTGCTGGC AGATATCTTC AGCTCCTTCT CTTTCCTACT 
GGCCACAGTG GCCGGAGCAT GCCAGCTGTT CTGCATCAGC CTCTGCTTCC TTCCCTGGGT 
CTCCTTTCTT CAGGCTTCGC TCCCAGAAGC CGTTTGCAAG CCTCCCTTCT TCCTGCTGCC 
AGATGGTCTC TGTTGATCAC AGTTCAGCCT CCTGGTGTGC CTCCCTTCTC CCTCCAGCCA 
GTGACTCCTG ACCACCAAGC CACACACACC CCCTCGCTGC CAACACCTCT TCAGTTTTTT 
TATATTTATT TTATTATTTT ATATGTTATG TTTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515426.1
CDS	239..2257
Translation	MRSIMSQESSYGKWTISSSDESEDEKPKPDKPSASSHPQAGQGVSKELIYTCSEARKVAHKRQISPVKFNDADSVLPHKKQKSDSPEGLGWCLSSSDDDQQPDVTQQEQPKRVLPQEKKHVSSPDVTTAQKVVDRSPPASLRPQRADDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGIKAKYNSKALHIKDILSPLFGTLVSSAQFNYCFDVDWLIKQYPPEFRKNPILLVHGDKREAKADLHAQAKPYANISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIREDWHQKTQGIWLSPLYPRIDQGSHTAGESSTRFKADLTSYLTAYNAPPLQEWIDIIQEHDLSETNVYLIGSTPGRFQGSHRDNWGHFRLRKLLQAHAPSTPKGECWPIVGQFSSIGSLGPDESKWLCSEFKDSLLALREEGRPPGKSAVPLHLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQRWLHSYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFSSPVLLHPRGFQDGTAGRYLQLLLFPTGHSGRSMPAVLHQPLLPSLGLLSSGFAPRSRLQASLLPAARWSLLITVQPPGVPPFSLQPVTPDHQATHTPSLPTPLQFFYIYFIILYVMFP

Target ORF information:

RefSeq Version	XM_006515363.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515363.3

ORF Insert Sequence:

ATGAGAAGTA TAATGTCCCA AGAAAGCAGC TACGGGAAGT GGACAATATC CAGTAGTGAC 
GAAAGCGAGG ATGAGAAGCC CAAACCTGAT AAGCCGTCAG CATCCTCTCA CCCCCAGGCT 
GGGCAGGGAG TGTCGAAGGA GCTCATATAC ACCTGCTCAG AGGCCCGCAA GGTTGCACAC 
AAGAGACAGA TCTCACCTGT GAAGTTCAAC GACGCAGACT CGGTGCTACC CCACAAGAAG 
CAGAAGAGTG ACTCCCCGGA AGGTCTCGGC TGGTGTCTCT CCAGCAGCGA TGATGACCAG 
CAGCCAGATG TGACACAGCA GGAACAGCCT AAGAGAGTGC TGCCTCAAGA GAAAAAACAT 
GTCTCCTCAC CCGATGTGAC TACTGCCCAG AAAGTTGTGG ATAGAAGCCC TCCTGCCAGC 
CTTAGGCCCC AAAGGGCAGA CGATGAGTAC GAGACCTCAG GGGAAGGCCA GGACATCTGG 
GACATGCTGG ATAAGGGGAA CCCCTTCCAG TTTTATCTCA CTAGAGTGTC GGGGATTAAG 
GCAAAGTACA ACTCCAAAGC CCTCCACATC AAGGATATTT TATCCCCTCT GTTTGGGACA 
CTTGTCTCTT CAGCTCAGTT TAACTACTGC TTTGACGTGG ACTGGCTCAT AAAGCAATAT 
CCACCGGAAT TCAGGAAGAA CCCGATCCTG CTTGTGCACG GTGATAAGAG GGAAGCCAAG 
GCAGACTTAC ATGCCCAGGC GAAGCCCTAT GCGAACATTT CCCTCTGCCA GGCAAAGTTG 
GATATTGCAT TTGGAACACA CCACACGAAA ATGATGCTCT TGCTCTATGA AGAAGGCCTC 
CGAGTTGTCA TCCACACCTC CAACCTCATC CGGGAAGACT GGCACCAGAA AACCCAAGGA 
ATATGGTTGA GCCCTTTGTA CCCAAGAATT GACCAGGGAA GCCACACAGC TGGAGAGTCA 
AGCACCCGTT TCAAAGCTGA CCTCACCAGT TATCTAACGG CGTATAACGC TCCCCCTCTG 
CAGGAGTGGA TAGACATCAT CCAAGAGCAT GACCTCTCTG AGACCAATGT TTACCTCATT 
GGTTCAACCC CTGGACGCTT TCAAGGAAGT CACAGAGATA ACTGGGGACA TTTTAGGCTT 
AGAAAGCTTC TGCAAGCGCA TGCCCCGTCC ACACCCAAGG GCGAGTGCTG GCCTATCGTG 
GGTCAGTTCT CAAGCATCGG CTCCCTGGGG CCCGATGAAT CCAAGTGGCT GTGCTCGGAG 
TTTAAAGACA GCTTGCTGGC ACTGAGAGAG GAAGGCCGAC CTCCGGGCAA GAGTGCAGTT 
CCTCTTCATC TGATCTATCC CTCTGTGGAA AATGTGCGGA CCAGCTTAGA AGGATACCCT 
GCTGGGGGCT CTCTTCCCTA TAGCATCCAG ACGGCTGAAA AGCAGAGGTG GCTGCACTCC 
TACTTCCACA AGTGGTCAGC AGAGACATCC GGCCGAAGTA ATGCCATGCC ACACATTAAG 
ACGTACATGA GGCCCTCGCC AGACTTCAGT AAACTGGCTT GGTTCCTGGT CACAAGTGCG 
AATCTGTCCA AGGCTGCCTG GGGGGCACTG GAGAAGAACG GCACCCAGCT GATGATACGC 
TCCTACGAGC TCGGGGTCCT CTTCCTCCCG TCTGCGTTTT CCTCACCTGT CCTCCTCCAC 
CCCAGAGGGT TTCAGGATGG AACTGCTGGC AGATATCTTC AGCTCCTTCT CTTTCCTACT 
GGCCACAGTG GCCGGAGCAT GCCAGCTGTT CTGCATCAGC CTCTGCTTCC TTCCCTGGGT 
CTCCTTTCTT CAGGCTTCGC TCCCAGAAGC CGTTTGCAAG CCTCCCTTCT TCCTGCTGCC 
AGATGGTCTC TGTTGATCAC AGTTCAGCCT CCTGGTGTGC CTCCCTTCTC CCTCCAGCCA 
GTGACTCCTG ACCACCAAGC CACACACACC CCCTCGCTGC CAACACCTCT TCAGTTTTTT 
TATATTTATT TTATTATTTT ATATGTTATG TTTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tdp1 ( XM_006515364.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515427.1 Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu47509
Clone ID Related Accession (Same CDS sequence)	XM_006515364.3 , XM_006515364.4
Accession Version	XM_006515364.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tyrosyl-DNA phosphodiesterase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515364.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAAG AAAGCAGCTA CGGGAAGTGG ACAATATCCA GTAGTGACGA AAGCGAGGAT 
GAGAAGCCCA AACCTGATAA GCCGTCAGCA TCCTCTCACC CCCAGGCTGG GCAGGGAGTG 
TCGAAGGAGC TCATATACAC CTGCTCAGAG GCCCGCAAGG TTGCACACAA GAGACAGATC 
TCACCTGTGA AGTTCAACGA CGCAGACTCG GTGCTACCCC ACAAGAAGCA GAAGAGTGAC 
TCCCCGGAAG GTCTCGGCTG GTGTCTCTCC AGCAGCGATG ATGACCAGCA GCCAGATGTG 
ACACAGCAGG AACAGCCTAA GAGAGTGCTG CCTCAAGAGA AAAAACATGT CTCCTCACCC 
GATGTGACTA CTGCCCAGAA AGTTGTGGAT AGAAGCCCTC CTGCCAGCCT TAGGCCCCAA 
AGGGCAGACG ATGAGTACGA GACCTCAGGG GAAGGCCAGG ACATCTGGGA CATGCTGGAT 
AAGGGGAACC CCTTCCAGTT TTATCTCACT AGAGTGTCGG GGATTAAGGC AAAGTACAAC 
TCCAAAGCCC TCCACATCAA GGATATTTTA TCCCCTCTGT TTGGGACACT TGTCTCTTCA 
GCTCAGTTTA ACTACTGCTT TGACGTGGAC TGGCTCATAA AGCAATATCC ACCGGAATTC 
AGGAAGAACC CGATCCTGCT TGTGCACGGT GATAAGAGGG AAGCCAAGGC AGACTTACAT 
GCCCAGGCGA AGCCCTATGC GAACATTTCC CTCTGCCAGG CAAAGTTGGA TATTGCATTT 
GGAACACACC ACACGAAAAT GATGCTCTTG CTCTATGAAG AAGGCCTCCG AGTTGTCATC 
CACACCTCCA ACCTCATCCG GGAAGACTGG CACCAGAAAA CCCAAGGAAT ATGGTTGAGC 
CCTTTGTACC CAAGAATTGA CCAGGGAAGC CACACAGCTG GAGAGTCAAG CACCCGTTTC 
AAAGCTGACC TCACCAGTTA TCTAACGGCG TATAACGCTC CCCCTCTGCA GGAGTGGATA 
GACATCATCC AAGAGCATGA CCTCTCTGAG ACCAATGTTT ACCTCATTGG TTCAACCCCT 
GGACGCTTTC AAGGAAGTCA CAGAGATAAC TGGGGACATT TTAGGCTTAG AAAGCTTCTG 
CAAGCGCATG CCCCGTCCAC ACCCAAGGGC GAGTGCTGGC CTATCGTGGG TCAGTTCTCA 
AGCATCGGCT CCCTGGGGCC CGATGAATCC AAGTGGCTGT GCTCGGAGTT TAAAGACAGC 
TTGCTGGCAC TGAGAGAGGA AGGCCGACCT CCGGGCAAGA GTGCAGTTCC TCTTCATCTG 
ATCTATCCCT CTGTGGAAAA TGTGCGGACC AGCTTAGAAG GATACCCTGC TGGGGGCTCT 
CTTCCCTATA GCATCCAGAC GGCTGAAAAG CAGAGGTGGC TGCACTCCTA CTTCCACAAG 
TGGTCAGCAG AGACATCCGG CCGAAGTAAT GCCATGCCAC ACATTAAGAC GTACATGAGG 
CCCTCGCCAG ACTTCAGTAA ACTGGCTTGG TTCCTGGTCA CAAGTGCGAA TCTGTCCAAG 
GCTGCCTGGG GGGCACTGGA GAAGAACGGC ACCCAGCTGA TGATACGCTC CTACGAGCTC 
GGGGTCCTCT TCCTCCCGTC TGCGTTTTCC TCACCTGTCC TCCTCCACCC CAGAGGGTTT 
CAGGATGGAA CTGCTGGCAG ATATCTTCAG CTCCTTCTCT TTCCTACTGG CCACAGTGGC 
CGGAGCATGC CAGCTGTTCT GCATCAGCCT CTGCTTCCTT CCCTGGGTCT CCTTTCTTCA 
GGCTTCGCTC CCAGAAGCCG TTTGCAAGCC TCCCTTCTTC CTGCTGCCAG ATGGTCTCTG 
TTGATCACAG TTCAGCCTCC TGGTGTGCCT CCCTTCTCCC TCCAGCCAGT GACTCCTGAC 
CACCAAGCCA CACACACCCC CTCGCTGCCA ACACCTCTTC AGTTTTTTTA TATTTATTTT 
ATTATTTTAT ATGTTATGTT TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515427.1
CDS	45..2051
Translation	MSQESSYGKWTISSSDESEDEKPKPDKPSASSHPQAGQGVSKELIYTCSEARKVAHKRQISPVKFNDADSVLPHKKQKSDSPEGLGWCLSSSDDDQQPDVTQQEQPKRVLPQEKKHVSSPDVTTAQKVVDRSPPASLRPQRADDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGIKAKYNSKALHIKDILSPLFGTLVSSAQFNYCFDVDWLIKQYPPEFRKNPILLVHGDKREAKADLHAQAKPYANISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIREDWHQKTQGIWLSPLYPRIDQGSHTAGESSTRFKADLTSYLTAYNAPPLQEWIDIIQEHDLSETNVYLIGSTPGRFQGSHRDNWGHFRLRKLLQAHAPSTPKGECWPIVGQFSSIGSLGPDESKWLCSEFKDSLLALREEGRPPGKSAVPLHLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQRWLHSYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFSSPVLLHPRGFQDGTAGRYLQLLLFPTGHSGRSMPAVLHQPLLPSLGLLSSGFAPRSRLQASLLPAARWSLLITVQPPGVPPFSLQPVTPDHQATHTPSLPTPLQFFYIYFIILYVMFP

Target ORF information:

RefSeq Version	XM_006515364.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515364.3

ORF Insert Sequence:

ATGTCCCAAG AAAGCAGCTA CGGGAAGTGG ACAATATCCA GTAGTGACGA AAGCGAGGAT 
GAGAAGCCCA AACCTGATAA GCCGTCAGCA TCCTCTCACC CCCAGGCTGG GCAGGGAGTG 
TCGAAGGAGC TCATATACAC CTGCTCAGAG GCCCGCAAGG TTGCACACAA GAGACAGATC 
TCACCTGTGA AGTTCAACGA CGCAGACTCG GTGCTACCCC ACAAGAAGCA GAAGAGTGAC 
TCCCCGGAAG GTCTCGGCTG GTGTCTCTCC AGCAGCGATG ATGACCAGCA GCCAGATGTG 
ACACAGCAGG AACAGCCTAA GAGAGTGCTG CCTCAAGAGA AAAAACATGT CTCCTCACCC 
GATGTGACTA CTGCCCAGAA AGTTGTGGAT AGAAGCCCTC CTGCCAGCCT TAGGCCCCAA 
AGGGCAGACG ATGAGTACGA GACCTCAGGG GAAGGCCAGG ACATCTGGGA CATGCTGGAT 
AAGGGGAACC CCTTCCAGTT TTATCTCACT AGAGTGTCGG GGATTAAGGC AAAGTACAAC 
TCCAAAGCCC TCCACATCAA GGATATTTTA TCCCCTCTGT TTGGGACACT TGTCTCTTCA 
GCTCAGTTTA ACTACTGCTT TGACGTGGAC TGGCTCATAA AGCAATATCC ACCGGAATTC 
AGGAAGAACC CGATCCTGCT TGTGCACGGT GATAAGAGGG AAGCCAAGGC AGACTTACAT 
GCCCAGGCGA AGCCCTATGC GAACATTTCC CTCTGCCAGG CAAAGTTGGA TATTGCATTT 
GGAACACACC ACACGAAAAT GATGCTCTTG CTCTATGAAG AAGGCCTCCG AGTTGTCATC 
CACACCTCCA ACCTCATCCG GGAAGACTGG CACCAGAAAA CCCAAGGAAT ATGGTTGAGC 
CCTTTGTACC CAAGAATTGA CCAGGGAAGC CACACAGCTG GAGAGTCAAG CACCCGTTTC 
AAAGCTGACC TCACCAGTTA TCTAACGGCG TATAACGCTC CCCCTCTGCA GGAGTGGATA 
GACATCATCC AAGAGCATGA CCTCTCTGAG ACCAATGTTT ACCTCATTGG TTCAACCCCT 
GGACGCTTTC AAGGAAGTCA CAGAGATAAC TGGGGACATT TTAGGCTTAG AAAGCTTCTG 
CAAGCGCATG CCCCGTCCAC ACCCAAGGGC GAGTGCTGGC CTATCGTGGG TCAGTTCTCA 
AGCATCGGCT CCCTGGGGCC CGATGAATCC AAGTGGCTGT GCTCGGAGTT TAAAGACAGC 
TTGCTGGCAC TGAGAGAGGA AGGCCGACCT CCGGGCAAGA GTGCAGTTCC TCTTCATCTG 
ATCTATCCCT CTGTGGAAAA TGTGCGGACC AGCTTAGAAG GATACCCTGC TGGGGGCTCT 
CTTCCCTATA GCATCCAGAC GGCTGAAAAG CAGAGGTGGC TGCACTCCTA CTTCCACAAG 
TGGTCAGCAG AGACATCCGG CCGAAGTAAT GCCATGCCAC ACATTAAGAC GTACATGAGG 
CCCTCGCCAG ACTTCAGTAA ACTGGCTTGG TTCCTGGTCA CAAGTGCGAA TCTGTCCAAG 
GCTGCCTGGG GGGCACTGGA GAAGAACGGC ACCCAGCTGA TGATACGCTC CTACGAGCTC 
GGGGTCCTCT TCCTCCCGTC TGCGTTTTCC TCACCTGTCC TCCTCCACCC CAGAGGGTTT 
CAGGATGGAA CTGCTGGCAG ATATCTTCAG CTCCTTCTCT TTCCTACTGG CCACAGTGGC 
CGGAGCATGC CAGCTGTTCT GCATCAGCCT CTGCTTCCTT CCCTGGGTCT CCTTTCTTCA 
GGCTTCGCTC CCAGAAGCCG TTTGCAAGCC TCCCTTCTTC CTGCTGCCAG ATGGTCTCTG 
TTGATCACAG TTCAGCCTCC TGGTGTGCCT CCCTTCTCCC TCCAGCCAGT GACTCCTGAC 
CACCAAGCCA CACACACCCC CTCGCTGCCA ACACCTCTTC AGTTTTTTTA TATTTATTTT 
ATTATTTTAT ATGTTATGTT TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tdp1 ( XM_030246506.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102366.1 Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47508
Clone ID Related Accession (Same CDS sequence)	XM_006515363.3 , XM_030246506.1
Accession Version	XM_030246506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tyrosyl-DNA phosphodiesterase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTA TAATGTCCCA AGAAAGCAGC TACGGGAAGT GGACAATATC CAGTAGTGAC 
GAAAGCGAGG ATGAGAAGCC CAAACCTGAT AAGCCGTCAG CATCCTCTCA CCCCCAGGCT 
GGGCAGGGAG TGTCGAAGGA GCTCATATAC ACCTGCTCAG AGGCCCGCAA GGTTGCACAC 
AAGAGACAGA TCTCACCTGT GAAGTTCAAC GACGCAGACT CGGTGCTACC CCACAAGAAG 
CAGAAGAGTG ACTCCCCGGA AGGTCTCGGC TGGTGTCTCT CCAGCAGCGA TGATGACCAG 
CAGCCAGATG TGACACAGCA GGAACAGCCT AAGAGAGTGC TGCCTCAAGA GAAAAAACAT 
GTCTCCTCAC CCGATGTGAC TACTGCCCAG AAAGTTGTGG ATAGAAGCCC TCCTGCCAGC 
CTTAGGCCCC AAAGGGCAGA CGATGAGTAC GAGACCTCAG GGGAAGGCCA GGACATCTGG 
GACATGCTGG ATAAGGGGAA CCCCTTCCAG TTTTATCTCA CTAGAGTGTC GGGGATTAAG 
GCAAAGTACA ACTCCAAAGC CCTCCACATC AAGGATATTT TATCCCCTCT GTTTGGGACA 
CTTGTCTCTT CAGCTCAGTT TAACTACTGC TTTGACGTGG ACTGGCTCAT AAAGCAATAT 
CCACCGGAAT TCAGGAAGAA CCCGATCCTG CTTGTGCACG GTGATAAGAG GGAAGCCAAG 
GCAGACTTAC ATGCCCAGGC GAAGCCCTAT GCGAACATTT CCCTCTGCCA GGCAAAGTTG 
GATATTGCAT TTGGAACACA CCACACGAAA ATGATGCTCT TGCTCTATGA AGAAGGCCTC 
CGAGTTGTCA TCCACACCTC CAACCTCATC CGGGAAGACT GGCACCAGAA AACCCAAGGA 
ATATGGTTGA GCCCTTTGTA CCCAAGAATT GACCAGGGAA GCCACACAGC TGGAGAGTCA 
AGCACCCGTT TCAAAGCTGA CCTCACCAGT TATCTAACGG CGTATAACGC TCCCCCTCTG 
CAGGAGTGGA TAGACATCAT CCAAGAGCAT GACCTCTCTG AGACCAATGT TTACCTCATT 
GGTTCAACCC CTGGACGCTT TCAAGGAAGT CACAGAGATA ACTGGGGACA TTTTAGGCTT 
AGAAAGCTTC TGCAAGCGCA TGCCCCGTCC ACACCCAAGG GCGAGTGCTG GCCTATCGTG 
GGTCAGTTCT CAAGCATCGG CTCCCTGGGG CCCGATGAAT CCAAGTGGCT GTGCTCGGAG 
TTTAAAGACA GCTTGCTGGC ACTGAGAGAG GAAGGCCGAC CTCCGGGCAA GAGTGCAGTT 
CCTCTTCATC TGATCTATCC CTCTGTGGAA AATGTGCGGA CCAGCTTAGA AGGATACCCT 
GCTGGGGGCT CTCTTCCCTA TAGCATCCAG ACGGCTGAAA AGCAGAGGTG GCTGCACTCC 
TACTTCCACA AGTGGTCAGC AGAGACATCC GGCCGAAGTA ATGCCATGCC ACACATTAAG 
ACGTACATGA GGCCCTCGCC AGACTTCAGT AAACTGGCTT GGTTCCTGGT CACAAGTGCG 
AATCTGTCCA AGGCTGCCTG GGGGGCACTG GAGAAGAACG GCACCCAGCT GATGATACGC 
TCCTACGAGC TCGGGGTCCT CTTCCTCCCG TCTGCGTTTT CCTCACCTGT CCTCCTCCAC 
CCCAGAGGGT TTCAGGATGG AACTGCTGGC AGATATCTTC AGCTCCTTCT CTTTCCTACT 
GGCCACAGTG GCCGGAGCAT GCCAGCTGTT CTGCATCAGC CTCTGCTTCC TTCCCTGGGT 
CTCCTTTCTT CAGGCTTCGC TCCCAGAAGC CGTTTGCAAG CCTCCCTTCT TCCTGCTGCC 
AGATGGTCTC TGTTGATCAC AGTTCAGCCT CCTGGTGTGC CTCCCTTCTC CCTCCAGCCA 
GTGACTCCTG ACCACCAAGC CACACACACC CCCTCGCTGC CAACACCTCT TCAGTTTTTT 
TATATTTATT TTATTATTTT ATATGTTATG TTTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102366.1
CDS	277..2295
Translation	MRSIMSQESSYGKWTISSSDESEDEKPKPDKPSASSHPQAGQGVSKELIYTCSEARKVAHKRQISPVKFNDADSVLPHKKQKSDSPEGLGWCLSSSDDDQQPDVTQQEQPKRVLPQEKKHVSSPDVTTAQKVVDRSPPASLRPQRADDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGIKAKYNSKALHIKDILSPLFGTLVSSAQFNYCFDVDWLIKQYPPEFRKNPILLVHGDKREAKADLHAQAKPYANISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIREDWHQKTQGIWLSPLYPRIDQGSHTAGESSTRFKADLTSYLTAYNAPPLQEWIDIIQEHDLSETNVYLIGSTPGRFQGSHRDNWGHFRLRKLLQAHAPSTPKGECWPIVGQFSSIGSLGPDESKWLCSEFKDSLLALREEGRPPGKSAVPLHLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQRWLHSYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFSSPVLLHPRGFQDGTAGRYLQLLLFPTGHSGRSMPAVLHQPLLPSLGLLSSGFAPRSRLQASLLPAARWSLLITVQPPGVPPFSLQPVTPDHQATHTPSLPTPLQFFYIYFIILYVMFP

Target ORF information:

RefSeq Version	XM_030246506.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246506.1

ORF Insert Sequence:

ATGAGAAGTA TAATGTCCCA AGAAAGCAGC TACGGGAAGT GGACAATATC CAGTAGTGAC 
GAAAGCGAGG ATGAGAAGCC CAAACCTGAT AAGCCGTCAG CATCCTCTCA CCCCCAGGCT 
GGGCAGGGAG TGTCGAAGGA GCTCATATAC ACCTGCTCAG AGGCCCGCAA GGTTGCACAC 
AAGAGACAGA TCTCACCTGT GAAGTTCAAC GACGCAGACT CGGTGCTACC CCACAAGAAG 
CAGAAGAGTG ACTCCCCGGA AGGTCTCGGC TGGTGTCTCT CCAGCAGCGA TGATGACCAG 
CAGCCAGATG TGACACAGCA GGAACAGCCT AAGAGAGTGC TGCCTCAAGA GAAAAAACAT 
GTCTCCTCAC CCGATGTGAC TACTGCCCAG AAAGTTGTGG ATAGAAGCCC TCCTGCCAGC 
CTTAGGCCCC AAAGGGCAGA CGATGAGTAC GAGACCTCAG GGGAAGGCCA GGACATCTGG 
GACATGCTGG ATAAGGGGAA CCCCTTCCAG TTTTATCTCA CTAGAGTGTC GGGGATTAAG 
GCAAAGTACA ACTCCAAAGC CCTCCACATC AAGGATATTT TATCCCCTCT GTTTGGGACA 
CTTGTCTCTT CAGCTCAGTT TAACTACTGC TTTGACGTGG ACTGGCTCAT AAAGCAATAT 
CCACCGGAAT TCAGGAAGAA CCCGATCCTG CTTGTGCACG GTGATAAGAG GGAAGCCAAG 
GCAGACTTAC ATGCCCAGGC GAAGCCCTAT GCGAACATTT CCCTCTGCCA GGCAAAGTTG 
GATATTGCAT TTGGAACACA CCACACGAAA ATGATGCTCT TGCTCTATGA AGAAGGCCTC 
CGAGTTGTCA TCCACACCTC CAACCTCATC CGGGAAGACT GGCACCAGAA AACCCAAGGA 
ATATGGTTGA GCCCTTTGTA CCCAAGAATT GACCAGGGAA GCCACACAGC TGGAGAGTCA 
AGCACCCGTT TCAAAGCTGA CCTCACCAGT TATCTAACGG CGTATAACGC TCCCCCTCTG 
CAGGAGTGGA TAGACATCAT CCAAGAGCAT GACCTCTCTG AGACCAATGT TTACCTCATT 
GGTTCAACCC CTGGACGCTT TCAAGGAAGT CACAGAGATA ACTGGGGACA TTTTAGGCTT 
AGAAAGCTTC TGCAAGCGCA TGCCCCGTCC ACACCCAAGG GCGAGTGCTG GCCTATCGTG 
GGTCAGTTCT CAAGCATCGG CTCCCTGGGG CCCGATGAAT CCAAGTGGCT GTGCTCGGAG 
TTTAAAGACA GCTTGCTGGC ACTGAGAGAG GAAGGCCGAC CTCCGGGCAA GAGTGCAGTT 
CCTCTTCATC TGATCTATCC CTCTGTGGAA AATGTGCGGA CCAGCTTAGA AGGATACCCT 
GCTGGGGGCT CTCTTCCCTA TAGCATCCAG ACGGCTGAAA AGCAGAGGTG GCTGCACTCC 
TACTTCCACA AGTGGTCAGC AGAGACATCC GGCCGAAGTA ATGCCATGCC ACACATTAAG 
ACGTACATGA GGCCCTCGCC AGACTTCAGT AAACTGGCTT GGTTCCTGGT CACAAGTGCG 
AATCTGTCCA AGGCTGCCTG GGGGGCACTG GAGAAGAACG GCACCCAGCT GATGATACGC 
TCCTACGAGC TCGGGGTCCT CTTCCTCCCG TCTGCGTTTT CCTCACCTGT CCTCCTCCAC 
CCCAGAGGGT TTCAGGATGG AACTGCTGGC AGATATCTTC AGCTCCTTCT CTTTCCTACT 
GGCCACAGTG GCCGGAGCAT GCCAGCTGTT CTGCATCAGC CTCTGCTTCC TTCCCTGGGT 
CTCCTTTCTT CAGGCTTCGC TCCCAGAAGC CGTTTGCAAG CCTCCCTTCT TCCTGCTGCC 
AGATGGTCTC TGTTGATCAC AGTTCAGCCT CCTGGTGTGC CTCCCTTCTC CCTCCAGCCA 
GTGACTCCTG ACCACCAAGC CACACACACC CCCTCGCTGC CAACACCTCT TCAGTTTTTT 
TATATTTATT TTATTATTTT ATATGTTATG TTTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tdp1 ( XM_006515364.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515427.1 Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu47509
Clone ID Related Accession (Same CDS sequence)	XM_006515364.3 , XM_006515364.4
Accession Version	XM_006515364.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tyrosyl-DNA phosphodiesterase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515364.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAAG AAAGCAGCTA CGGGAAGTGG ACAATATCCA GTAGTGACGA AAGCGAGGAT 
GAGAAGCCCA AACCTGATAA GCCGTCAGCA TCCTCTCACC CCCAGGCTGG GCAGGGAGTG 
TCGAAGGAGC TCATATACAC CTGCTCAGAG GCCCGCAAGG TTGCACACAA GAGACAGATC 
TCACCTGTGA AGTTCAACGA CGCAGACTCG GTGCTACCCC ACAAGAAGCA GAAGAGTGAC 
TCCCCGGAAG GTCTCGGCTG GTGTCTCTCC AGCAGCGATG ATGACCAGCA GCCAGATGTG 
ACACAGCAGG AACAGCCTAA GAGAGTGCTG CCTCAAGAGA AAAAACATGT CTCCTCACCC 
GATGTGACTA CTGCCCAGAA AGTTGTGGAT AGAAGCCCTC CTGCCAGCCT TAGGCCCCAA 
AGGGCAGACG ATGAGTACGA GACCTCAGGG GAAGGCCAGG ACATCTGGGA CATGCTGGAT 
AAGGGGAACC CCTTCCAGTT TTATCTCACT AGAGTGTCGG GGATTAAGGC AAAGTACAAC 
TCCAAAGCCC TCCACATCAA GGATATTTTA TCCCCTCTGT TTGGGACACT TGTCTCTTCA 
GCTCAGTTTA ACTACTGCTT TGACGTGGAC TGGCTCATAA AGCAATATCC ACCGGAATTC 
AGGAAGAACC CGATCCTGCT TGTGCACGGT GATAAGAGGG AAGCCAAGGC AGACTTACAT 
GCCCAGGCGA AGCCCTATGC GAACATTTCC CTCTGCCAGG CAAAGTTGGA TATTGCATTT 
GGAACACACC ACACGAAAAT GATGCTCTTG CTCTATGAAG AAGGCCTCCG AGTTGTCATC 
CACACCTCCA ACCTCATCCG GGAAGACTGG CACCAGAAAA CCCAAGGAAT ATGGTTGAGC 
CCTTTGTACC CAAGAATTGA CCAGGGAAGC CACACAGCTG GAGAGTCAAG CACCCGTTTC 
AAAGCTGACC TCACCAGTTA TCTAACGGCG TATAACGCTC CCCCTCTGCA GGAGTGGATA 
GACATCATCC AAGAGCATGA CCTCTCTGAG ACCAATGTTT ACCTCATTGG TTCAACCCCT 
GGACGCTTTC AAGGAAGTCA CAGAGATAAC TGGGGACATT TTAGGCTTAG AAAGCTTCTG 
CAAGCGCATG CCCCGTCCAC ACCCAAGGGC GAGTGCTGGC CTATCGTGGG TCAGTTCTCA 
AGCATCGGCT CCCTGGGGCC CGATGAATCC AAGTGGCTGT GCTCGGAGTT TAAAGACAGC 
TTGCTGGCAC TGAGAGAGGA AGGCCGACCT CCGGGCAAGA GTGCAGTTCC TCTTCATCTG 
ATCTATCCCT CTGTGGAAAA TGTGCGGACC AGCTTAGAAG GATACCCTGC TGGGGGCTCT 
CTTCCCTATA GCATCCAGAC GGCTGAAAAG CAGAGGTGGC TGCACTCCTA CTTCCACAAG 
TGGTCAGCAG AGACATCCGG CCGAAGTAAT GCCATGCCAC ACATTAAGAC GTACATGAGG 
CCCTCGCCAG ACTTCAGTAA ACTGGCTTGG TTCCTGGTCA CAAGTGCGAA TCTGTCCAAG 
GCTGCCTGGG GGGCACTGGA GAAGAACGGC ACCCAGCTGA TGATACGCTC CTACGAGCTC 
GGGGTCCTCT TCCTCCCGTC TGCGTTTTCC TCACCTGTCC TCCTCCACCC CAGAGGGTTT 
CAGGATGGAA CTGCTGGCAG ATATCTTCAG CTCCTTCTCT TTCCTACTGG CCACAGTGGC 
CGGAGCATGC CAGCTGTTCT GCATCAGCCT CTGCTTCCTT CCCTGGGTCT CCTTTCTTCA 
GGCTTCGCTC CCAGAAGCCG TTTGCAAGCC TCCCTTCTTC CTGCTGCCAG ATGGTCTCTG 
TTGATCACAG TTCAGCCTCC TGGTGTGCCT CCCTTCTCCC TCCAGCCAGT GACTCCTGAC 
CACCAAGCCA CACACACCCC CTCGCTGCCA ACACCTCTTC AGTTTTTTTA TATTTATTTT 
ATTATTTTAT ATGTTATGTT TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515427.1
CDS	98..2104
Translation	MSQESSYGKWTISSSDESEDEKPKPDKPSASSHPQAGQGVSKELIYTCSEARKVAHKRQISPVKFNDADSVLPHKKQKSDSPEGLGWCLSSSDDDQQPDVTQQEQPKRVLPQEKKHVSSPDVTTAQKVVDRSPPASLRPQRADDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGIKAKYNSKALHIKDILSPLFGTLVSSAQFNYCFDVDWLIKQYPPEFRKNPILLVHGDKREAKADLHAQAKPYANISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIREDWHQKTQGIWLSPLYPRIDQGSHTAGESSTRFKADLTSYLTAYNAPPLQEWIDIIQEHDLSETNVYLIGSTPGRFQGSHRDNWGHFRLRKLLQAHAPSTPKGECWPIVGQFSSIGSLGPDESKWLCSEFKDSLLALREEGRPPGKSAVPLHLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQRWLHSYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFSSPVLLHPRGFQDGTAGRYLQLLLFPTGHSGRSMPAVLHQPLLPSLGLLSSGFAPRSRLQASLLPAARWSLLITVQPPGVPPFSLQPVTPDHQATHTPSLPTPLQFFYIYFIILYVMFP

Target ORF information:

RefSeq Version	XM_006515364.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515364.4

ORF Insert Sequence:

ATGTCCCAAG AAAGCAGCTA CGGGAAGTGG ACAATATCCA GTAGTGACGA AAGCGAGGAT 
GAGAAGCCCA AACCTGATAA GCCGTCAGCA TCCTCTCACC CCCAGGCTGG GCAGGGAGTG 
TCGAAGGAGC TCATATACAC CTGCTCAGAG GCCCGCAAGG TTGCACACAA GAGACAGATC 
TCACCTGTGA AGTTCAACGA CGCAGACTCG GTGCTACCCC ACAAGAAGCA GAAGAGTGAC 
TCCCCGGAAG GTCTCGGCTG GTGTCTCTCC AGCAGCGATG ATGACCAGCA GCCAGATGTG 
ACACAGCAGG AACAGCCTAA GAGAGTGCTG CCTCAAGAGA AAAAACATGT CTCCTCACCC 
GATGTGACTA CTGCCCAGAA AGTTGTGGAT AGAAGCCCTC CTGCCAGCCT TAGGCCCCAA 
AGGGCAGACG ATGAGTACGA GACCTCAGGG GAAGGCCAGG ACATCTGGGA CATGCTGGAT 
AAGGGGAACC CCTTCCAGTT TTATCTCACT AGAGTGTCGG GGATTAAGGC AAAGTACAAC 
TCCAAAGCCC TCCACATCAA GGATATTTTA TCCCCTCTGT TTGGGACACT TGTCTCTTCA 
GCTCAGTTTA ACTACTGCTT TGACGTGGAC TGGCTCATAA AGCAATATCC ACCGGAATTC 
AGGAAGAACC CGATCCTGCT TGTGCACGGT GATAAGAGGG AAGCCAAGGC AGACTTACAT 
GCCCAGGCGA AGCCCTATGC GAACATTTCC CTCTGCCAGG CAAAGTTGGA TATTGCATTT 
GGAACACACC ACACGAAAAT GATGCTCTTG CTCTATGAAG AAGGCCTCCG AGTTGTCATC 
CACACCTCCA ACCTCATCCG GGAAGACTGG CACCAGAAAA CCCAAGGAAT ATGGTTGAGC 
CCTTTGTACC CAAGAATTGA CCAGGGAAGC CACACAGCTG GAGAGTCAAG CACCCGTTTC 
AAAGCTGACC TCACCAGTTA TCTAACGGCG TATAACGCTC CCCCTCTGCA GGAGTGGATA 
GACATCATCC AAGAGCATGA CCTCTCTGAG ACCAATGTTT ACCTCATTGG TTCAACCCCT 
GGACGCTTTC AAGGAAGTCA CAGAGATAAC TGGGGACATT TTAGGCTTAG AAAGCTTCTG 
CAAGCGCATG CCCCGTCCAC ACCCAAGGGC GAGTGCTGGC CTATCGTGGG TCAGTTCTCA 
AGCATCGGCT CCCTGGGGCC CGATGAATCC AAGTGGCTGT GCTCGGAGTT TAAAGACAGC 
TTGCTGGCAC TGAGAGAGGA AGGCCGACCT CCGGGCAAGA GTGCAGTTCC TCTTCATCTG 
ATCTATCCCT CTGTGGAAAA TGTGCGGACC AGCTTAGAAG GATACCCTGC TGGGGGCTCT 
CTTCCCTATA GCATCCAGAC GGCTGAAAAG CAGAGGTGGC TGCACTCCTA CTTCCACAAG 
TGGTCAGCAG AGACATCCGG CCGAAGTAAT GCCATGCCAC ACATTAAGAC GTACATGAGG 
CCCTCGCCAG ACTTCAGTAA ACTGGCTTGG TTCCTGGTCA CAAGTGCGAA TCTGTCCAAG 
GCTGCCTGGG GGGCACTGGA GAAGAACGGC ACCCAGCTGA TGATACGCTC CTACGAGCTC 
GGGGTCCTCT TCCTCCCGTC TGCGTTTTCC TCACCTGTCC TCCTCCACCC CAGAGGGTTT 
CAGGATGGAA CTGCTGGCAG ATATCTTCAG CTCCTTCTCT TTCCTACTGG CCACAGTGGC 
CGGAGCATGC CAGCTGTTCT GCATCAGCCT CTGCTTCCTT CCCTGGGTCT CCTTTCTTCA 
GGCTTCGCTC CCAGAAGCCG TTTGCAAGCC TCCCTTCTTC CTGCTGCCAG ATGGTCTCTG 
TTGATCACAG TTCAGCCTCC TGGTGTGCCT CCCTTCTCCC TCCAGCCAGT GACTCCTGAC 
CACCAAGCCA CACACACCCC CTCGCTGCCA ACACCTCTTC AGTTTTTTTA TATTTATTTT 
ATTATTTTAT ATGTTATGTT TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tdp1 ( XM_030246507.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102367.1 Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X3, mRNA.

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CloneID	OMu85979
Clone ID Related Accession (Same CDS sequence)	XM_030246507.1
Accession Version	XM_030246507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tyrosyl-DNA phosphodiesterase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTGCA GTTTTCCTCG GAGCTTTGTA TTCATCTGTC ACAGGCAGCA CGTTAGGAGC 
GGCTGGAAAA CCGATTCAGC CCTTCCCAGA AGTATAATGT CCCAAGAAAG CAGCTACGGG 
AAGTGGACAA TATCCAGTAG TGACGAAAGC GAGGATGAGA AGCCCAAACC TGATAAGCCG 
TCAGCATCCT CTCACCCCCA GGCTGGGCAG GGAGTGTCGA AGGAGCTCAT ATACACCTGC 
TCAGAGGCCC GCAAGGTTGC ACACAAGAGA CAGATCTCAC CTGTGAAGTT CAACGACGCA 
GACTCGGTGC TACCCCACAA GAAGCAGAAG AGTGACTCCC CGGAAGGTCT CGGCTGGTGT 
CTCTCCAGCA GCGATGATGA CCAGCAGCCA GATGTGACAC AGCAGGAACA GCCTAAGAGA 
GTGCTGCCTC AAGAGAAAAA ACATGTCTCC TCACCCGATG TGACTACTGC CCAGAAAGTT 
GTGGATAGAA GCCCTCCTGC CAGCCTTAGG CCCCAAAGGG CAGACGATGA GTACGAGACC 
TCAGGGGAAG GCCAGGACAT CTGGGACATG CTGGATAAGG GGAACCCCTT CCAGTTTTAT 
CTCACTAGAG TGTCGGGGAT TAAGGCAAAG TACAACTCCA AAGCCCTCCA CATCAAGGAT 
ATTTTATCCC CTCTGTTTGG GACACTTGTC TCTTCAGCTC AGTTTAACTA CTGCTTTGAC 
GTGGACTGGC TCATAAAGCA ATATCCACCG GAATTCAGGA AGAACCCGAT CCTGCTTGTG 
CACGGTGATA AGAGGGAAGC CAAGGCAGAC TTACATGCCC AGGCGAAGCC CTATGCGAAC 
ATTTCCCTCT GCCAGGCAAA GTTGGATATT GCATTTGGAA CACACCACAC GAAAATGATG 
CTCTTGCTCT ATGAAGAAGG CCTCCGAGTT GTCATCCACA CCTCCAACCT CATCCGGGAA 
GACTGGCACC AGAAAACCCA AGGAATATGG TTGAGCCCTT TGTACCCAAG AATTGACCAG 
GGAAGCCACA CAGCTGGAGA GTCAAGCACC CGTTTCAAAG CTGACCTCAC CAGTTATCTA 
ACGGCGTATA ACGCTCCCCC TCTGCAGGAG TGGATAGACA TCATCCAAGA GCATGACCTC 
TCTGAGACCA ATGTTTACCT CATTGGTTCA ACCCCTGGAC GCTTTCAAGG AAGTCACAGA 
GATAACTGGG GACATTTTAG GCTTAGAAAG CTTCTGCAAG CGCATGCCCC GTCCACACCC 
AAGGGCGAGT GCTGGCCTAT CGTGGGTCAG TTCTCAAGCA TCGGCTCCCT GGGGCCCGAT 
GAATCCAAGT GGCTGTGCTC GGAGTTTAAA GACAGCTTGC TGGCACTGAG AGAGGAAGGC 
CGACCTCCGG GCAAGAGTGC AGTTCCTCTT CATCTGATCT ATCCCTCTGT GGAAAATGTG 
CGGACCAGCT TAGAAGGATA CCCTGCTGGG GGCTCTCTTC CCTATAGCAT CCAGACGGCT 
GAAAAGCAGA GGTGGCTGCA CTCCTACTTC CACAAGTGGT CAGCAGAGAC ATCCGGCCGA 
AGTAATGCCA TGCCACACAT TAAGACGTAC ATGAGGCCCT CGCCAGACTT CAGTAAACTG 
GCTTGGTTCC TGGTCACAAG TGCGAATCTG TCCAAGGCTG CCTGGGGGGC ACTGGAGAAG 
AACGGCACCC AGCTGATGAT ACGCTCCTAC GAGCTCGGGG TCCTCTTCCT CCCGTCTGCG 
TTTGGCCTGG ACACCTTCAA AGTGAAACAG AAATTCTTCT CCAGCAGCTG TGAGCCGACG 
GCCTCCTTTC CTGTGCCCTA TGACCTGCCC CCAGAGCTGT ACGGGAGTAA AGACCGGCCT 
TGGATTTGGA ACATTCCTTA TGTCAAAGCA CCTGACACGC ACGGCAACAT GTGGGTGCCC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102367.1
CDS	50..1975
Translation	MYCSFPRSFVFICHRQHVRSGWKTDSALPRSIMSQESSYGKWTISSSDESEDEKPKPDKPSASSHPQAGQGVSKELIYTCSEARKVAHKRQISPVKFNDADSVLPHKKQKSDSPEGLGWCLSSSDDDQQPDVTQQEQPKRVLPQEKKHVSSPDVTTAQKVVDRSPPASLRPQRADDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGIKAKYNSKALHIKDILSPLFGTLVSSAQFNYCFDVDWLIKQYPPEFRKNPILLVHGDKREAKADLHAQAKPYANISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIREDWHQKTQGIWLSPLYPRIDQGSHTAGESSTRFKADLTSYLTAYNAPPLQEWIDIIQEHDLSETNVYLIGSTPGRFQGSHRDNWGHFRLRKLLQAHAPSTPKGECWPIVGQFSSIGSLGPDESKWLCSEFKDSLLALREEGRPPGKSAVPLHLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQRWLHSYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFGLDTFKVKQKFFSSSCEPTASFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPS

Target ORF information:

RefSeq Version	XM_030246507.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tyrosyl-DNA phosphodiesterase 1 (Tdp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246507.1

ORF Insert Sequence:

ATGTATTGCA GTTTTCCTCG GAGCTTTGTA TTCATCTGTC ACAGGCAGCA CGTTAGGAGC 
GGCTGGAAAA CCGATTCAGC CCTTCCCAGA AGTATAATGT CCCAAGAAAG CAGCTACGGG 
AAGTGGACAA TATCCAGTAG TGACGAAAGC GAGGATGAGA AGCCCAAACC TGATAAGCCG 
TCAGCATCCT CTCACCCCCA GGCTGGGCAG GGAGTGTCGA AGGAGCTCAT ATACACCTGC 
TCAGAGGCCC GCAAGGTTGC ACACAAGAGA CAGATCTCAC CTGTGAAGTT CAACGACGCA 
GACTCGGTGC TACCCCACAA GAAGCAGAAG AGTGACTCCC CGGAAGGTCT CGGCTGGTGT 
CTCTCCAGCA GCGATGATGA CCAGCAGCCA GATGTGACAC AGCAGGAACA GCCTAAGAGA 
GTGCTGCCTC AAGAGAAAAA ACATGTCTCC TCACCCGATG TGACTACTGC CCAGAAAGTT 
GTGGATAGAA GCCCTCCTGC CAGCCTTAGG CCCCAAAGGG CAGACGATGA GTACGAGACC 
TCAGGGGAAG GCCAGGACAT CTGGGACATG CTGGATAAGG GGAACCCCTT CCAGTTTTAT 
CTCACTAGAG TGTCGGGGAT TAAGGCAAAG TACAACTCCA AAGCCCTCCA CATCAAGGAT 
ATTTTATCCC CTCTGTTTGG GACACTTGTC TCTTCAGCTC AGTTTAACTA CTGCTTTGAC 
GTGGACTGGC TCATAAAGCA ATATCCACCG GAATTCAGGA AGAACCCGAT CCTGCTTGTG 
CACGGTGATA AGAGGGAAGC CAAGGCAGAC TTACATGCCC AGGCGAAGCC CTATGCGAAC 
ATTTCCCTCT GCCAGGCAAA GTTGGATATT GCATTTGGAA CACACCACAC GAAAATGATG 
CTCTTGCTCT ATGAAGAAGG CCTCCGAGTT GTCATCCACA CCTCCAACCT CATCCGGGAA 
GACTGGCACC AGAAAACCCA AGGAATATGG TTGAGCCCTT TGTACCCAAG AATTGACCAG 
GGAAGCCACA CAGCTGGAGA GTCAAGCACC CGTTTCAAAG CTGACCTCAC CAGTTATCTA 
ACGGCGTATA ACGCTCCCCC TCTGCAGGAG TGGATAGACA TCATCCAAGA GCATGACCTC 
TCTGAGACCA ATGTTTACCT CATTGGTTCA ACCCCTGGAC GCTTTCAAGG AAGTCACAGA 
GATAACTGGG GACATTTTAG GCTTAGAAAG CTTCTGCAAG CGCATGCCCC GTCCACACCC 
AAGGGCGAGT GCTGGCCTAT CGTGGGTCAG TTCTCAAGCA TCGGCTCCCT GGGGCCCGAT 
GAATCCAAGT GGCTGTGCTC GGAGTTTAAA GACAGCTTGC TGGCACTGAG AGAGGAAGGC 
CGACCTCCGG GCAAGAGTGC AGTTCCTCTT CATCTGATCT ATCCCTCTGT GGAAAATGTG 
CGGACCAGCT TAGAAGGATA CCCTGCTGGG GGCTCTCTTC CCTATAGCAT CCAGACGGCT 
GAAAAGCAGA GGTGGCTGCA CTCCTACTTC CACAAGTGGT CAGCAGAGAC ATCCGGCCGA 
AGTAATGCCA TGCCACACAT TAAGACGTAC ATGAGGCCCT CGCCAGACTT CAGTAAACTG 
GCTTGGTTCC TGGTCACAAG TGCGAATCTG TCCAAGGCTG CCTGGGGGGC ACTGGAGAAG 
AACGGCACCC AGCTGATGAT ACGCTCCTAC GAGCTCGGGG TCCTCTTCCT CCCGTCTGCG 
TTTGGCCTGG ACACCTTCAA AGTGAAACAG AAATTCTTCT CCAGCAGCTG TGAGCCGACG 
GCCTCCTTTC CTGTGCCCTA TGACCTGCCC CCAGAGCTGT ACGGGAGTAA AGACCGGCCT 
TGGATTTGGA ACATTCCTTA TGTCAAAGCA CCTGACACGC ACGGCAACAT GTGGGTGCCC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial protein-linked DNA breaks perturb mitochondrial gene transcription and trigger free radical-induced DNA damage.
Science advances3(4)e1602506(2017 Apr)
Chiang SC,Meagher M,Kassouf N,Hafezparast M,McKinnon PJ,Haywood R,El-Khamisy SF

Aberrant topoisomerase-1 DNA lesions are pathogenic in neurodegenerative genome instability syndromes.
Nature neuroscience17(6)813-21(2014 Jun)
Katyal S,Lee Y,Nitiss KC,Downing SM,Li Y,Shimada M,Zhao J,Russell HR,Petrini JH,Nitiss JL,McKinnon PJ

R-loops in proliferating cells but not in the brain: implications for AOA2 and other autosomal recessive ataxias.
PloS one9(3)e90219(2014)
Yeo AJ,Becherel OJ,Luff JE,Cullen JK,Wongsurawat T,Jenjaroenpun P,Jenjaroenpoon P,Kuznetsov VA,McKinnon PJ,Lavin MF

Pol ? associated complex and base excision repair factors in mouse fibroblasts.
Nucleic acids research40(22)11571-82(2012 Dec)
Prasad R,Williams JG,Hou EW,Wilson SH

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Tdp1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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