Rtn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rtn1
Entrez Gene ID	104001
Full Name	reticulon 1
Synonyms	Nsp
General protein information	Preferred Names reticulon 1 Names reticulon-1 neuroendocrine-specific protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001286448.1	NP_001273377.1	reticulon-1 isoform A2
XM_006515323.2	XP_006515386.1	reticulon-1 isoform X1
XM_006515324.1	XP_006515387.1	reticulon-1 isoform X1
XM_030246489.1	XP_030102349.1	reticulon-1 isoform X1
XM_006515323.1	XP_006515386.1	reticulon-1 isoform X1
NM_153457.7	NP_703187.2	reticulon-1 isoform A
NM_001007596.2	NP_001007597.1	reticulon-1 isoform C
XM_006515324.2	XP_006515387.1	reticulon-1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RTN1	NP_001029293.1
	RTN1	XP_002805126.1
Danio rerio (zebrafish)	rtn1a	NP_001025138.2
Xenopus tropicalis (tropical clawed frog)	rtn1	NP_001078819.2
Homo sapiens (human)	RTN1	NP_066959.1
Bos taurus (cattle)	RTN1	XP_005212118.1
Gallus gallus (chicken)	RTN1	NP_001001466.1
Canis lupus familiaris (dog)	RTN1	XP_005623514.1
Mus musculus (house mouse)	Rtn1	NP_703187.2
Rattus norvegicus (Norway rat)	Rtn1	NP_446317.1

Gene Ontology
Bibliography

Related articles in PubMed

Reticulon-1 and Reduced Migration toward Chemoattractants by Macrophages Differentiated from the Bone Marrow of Ultraviolet-Irradiated and Ultraviolet-Chimeric Mice.
McGonigle TA, Dwyer AR, Greenland EL, Scott NM, Carter KW, Keane KN, Newsholme P, Goodridge HS, Pixley FJ, Hart PH
Journal of immunology (Baltimore, Md. : 1950)200(1)260-270(2018 Jan)

RTN1 and RTN3 protein are differentially associated with senile plaques in Alzheimer's brains.
Shi Q, Ge Y, He W, Hu X, Yan R
Scientific reports7(1)6145(2017 Jul)

Rtn1a-Mediated Endoplasmic Reticulum Stress in Podocyte Injury and Diabetic Nephropathy.
Fan Y, Zhang J, Xiao W, Lee K, Li Z, Wen J, He L, Gui D, Xue R, Jian G, Sheng X, He JC, Wang N
Scientific reports7(1)323(2017 Mar)

Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose Tissue.
Duteil D, Tosic M, Lausecker F, Nenseth HZ, M?ller JM, Urban S, Willmann D, Petroll K, Messaddeq N, Arrigoni L, Manke T, Kornfeld JW, Br?ning JC, Zagoriy V, Meret M, Dengjel J, Kanouni T, Sch?le R
Cell reports17(4)1008-1021(2016 10)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

reticulon-1 is responsible for reduced chemotaxis of macrophages differentiated from the one bone marrow of ultraviolet-irradiated and ultraviolet-chimeric mice
Title: Reticulon-1 and Reduced Migration toward Chemoattractants by Macrophages Differentiated from the Bone Marrow of Ultraviolet-Irradiated and Ultraviolet-Chimeric Mice.

RTN1A is a key mediator for proteinuria-induced tubular cell toxicity and renal fibrosis.
Title: Knockdown of RTN1A attenuates ER stress and kidney injury in albumin overload-induced nephropathy.

RTN1A contributes to progression of kidney disease by inducing ER stress.
Title: RTN1 mediates progression of kidney disease by inducing ER stress.

RTN-1C is able to specifically regulate gene expression, modulating transcript clusters which have been implicated in the onset of neurodegenerative disorders
Title: Characterization of gene expression induced by RTN-1C in human neuroblastoma cells and in mouse brain.

The following Rtn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rtn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09310	NM_001286448.1 Latest version!	Mus musculus reticulon 1 (Rtn1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu10814	NM_001007596.2 Latest version!	Mus musculus reticulon 1 (Rtn1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu02764	NM_153457.7 Latest version!	Mus musculus reticulon 1 (Rtn1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$461.30 $659.00
OMu47481	XM_006515323.2 Latest version!	Mus musculus reticulon 1 (Rtn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu47481	XM_030246489.1 Latest version!	Mus musculus reticulon 1 (Rtn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu47481	XM_006515324.2 Latest version!	Mus musculus reticulon 1 (Rtn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu47481	XM_006515323.1	Mus musculus reticulon 1 (Rtn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu47481	XM_006515324.1	Mus musculus reticulon 1 (Rtn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rtn1 ( XM_006515324.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515387.1 Mus musculus reticulon 1 (Rtn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu47481
Clone ID Related Accession (Same CDS sequence)	XM_006515323.2 , XM_006515324.1 , XM_030246489.1 , XM_006515323.1 , XM_006515324.2
Accession Version	XM_006515324.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	reticulon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG TTCCCGATGC CCTCGACCAC TCTCCCTCTT CAACACTAAA AGATGGGGAA 
GGGGCATGTT ACACATCCCT GATTTCTGAC GTCTGCTATC CACCTCGGGA GGACTCTGCA 
TACTTTACTG GAATTCTTCA GAAGGAAAAT GGTCACATCA CCACTTCCGA GAGCCCTGAG 
GAGCCGGAGA CCCCCGGACC CTCGTTACCA GAAGTGCCCG GGATGGAGCC ACAGGGCTTA 
TTGAGTTCTG ATTCTGGAAT AGAGATGACT CCTGCTGAGT CCACTGAGGT GAACAAGATC 
CTAGCTGACC CCCTAGACCA GATGAAGGCA GAAGCCTACA AATACATTGA CATAACCAGG 
CCACAGGAGG CAAAAGGCCA GGAGGAGCAA CACCCTGGGC TAGAAGATAA AGACTTGGAC 
TTTAAAGACA AGGACACTGA AGTCTCCACA AAAGCAGAAG GGGTTCGAGC ACCAAACCAG 
CCAGCTCCTG TGGAGGGGAA ACTCATCAAG GACCACTTAT TCGAAGAATC TACCTTTGCT 
CCTTACATAG ATGAGCTCTC TGATGAGCAG CACAGGGTGT CTCTGGTCAC TGCCCCTGTC 
AAGATCACAC TGACGGAGAT CGAACCTCCT CTTATGACTG CCACACAAGA GACAATCCCC 
GAGAAGCAAG ACCTGTGTCT GAAGCCAAGT CCTGACACAG TGCCCACTGT CACTGTCTCA 
GAGCCTGAAG ATGACAGCCC AGGATCTGTC ACGCCTCCAT CTTCTGGAAC AGAACCATCT 
GCTGCAGAGT CCCAGGGGAA AGGCAGCGTC TCCGAGGATG AGTTGATTGC TGCCATTAAA 
GAAGCAAAGG GGTTATCATA TGAAACCACC GAGAGTCCAA GGCCAGTGGG CCAGGTGGCC 
GACAAACCCA AAACCAAGAC CAGGTCTGGG CTGCCCACCA TCCCCAGTCC CCTCGACCAG 
GAGGCCAGCA GTGCCGAGTC CGGAGACTCA GAAATTGAGC TGGTGTCAGA GGACCCCATG 
GCCTCTGAGG ATGCCCTGCC CTCTGGCTAT GTGAGCTTCG GTCATGTGTC GGGCCCGCCC 
CCCTCTCCTG CCTCTCCATC CATCCAGTAC AGCATCCTGC GGGAGGAGCG TGAGGCCGAG 
CTGGACAGCG AGCTCATCAT CGAGTCCTGT GATGCCTCCT CAGCCTCGGA GGAGAGCCCC 
AAGCGCGAGC AGGACTCGCC CCCGATGAAA CCGGGTGCCC TGGACGCCAT CCGCGAGGAG 
ACAGGCTCTC GGGCCACCGA AGAACGAGCG CCCAGCCACC AAGGCCCTGT GGAGCCCGAC 
CCGATGCTCA GCTTTGCCCC CGCTGCCGCC CTCCAATCCC GCCCAGAGCC ATCGTCTGGA 
GATGGAGCCT CGGTCCCGGA GCCGCCCAGA TCACAGCAGC AGAAGCCTGA AGAGGAAGCT 
GTGAGTTCCA GTCAAAGTCC CACGGCCACC GAAATTCCTG GACCACTTGG TTCTGGCCTC 
ATGCCCCCTC TGCCTTTCTT CAACAAGCAA AAAGCTATTG ACCTTCTGTA CTGGCGGGAC 
ATCAAGCAGA CTGGGATTGT GTTCGGGAGC TTCCTGCTGC TGCTCTTCTC CCTGACCCAG 
TTCAGCGTTG TGAGCGTCGT TGCCTACCTG GCCCTGGCCG CCCTCTCTGC CACCATCAGC 
TTCCGCATCT ACAAGTCCGT TCTACAAGCT GTGCAGAAAA CAGATGAGGG TCACCCTTTC 
AAGGCCTACC TGGAGCTGGA GATCACCCTG TCCCAGGAGC AGATCCAGAA GTACACAGAC 
TGCCTGCAGC TGTATGTGAA CAGCACTCTG AAGGAGCTAC GGAGGCTTTT CCTGGTCCAG 
GACCTGGTGG ATTCCTTAAA ATTTGCAGTC CTCATGTGGC TCCTGACCTA CGTTGGCGCG 
CTCTTCAATG GCCTGACCCT GCTGCTTATG GCTGTGGTTT CGATGTTTAC TCTACCTGTT 
GTGTACGTTA AGCACCAGGC ACAAGTTGAC CAATATCTGG GACTTGTGAG GACTCACATA 
AACACCGTCG TGGCAAAGAT CCAGGCTAAA ATCCCCGGCG CCAAGAGGCA CGCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515387.1
CDS	141..2240
Translation	MAAVPDALDHSPSSTLKDGEGACYTSLISDVCYPPREDSAYFTGILQKENGHITTSESPEEPETPGPSLPEVPGMEPQGLLSSDSGIEMTPAESTEVNKILADPLDQMKAEAYKYIDITRPQEAKGQEEQHPGLEDKDLDFKDKDTEVSTKAEGVRAPNQPAPVEGKLIKDHLFEESTFAPYIDELSDEQHRVSLVTAPVKITLTEIEPPLMTATQETIPEKQDLCLKPSPDTVPTVTVSEPEDDSPGSVTPPSSGTEPSAAESQGKGSVSEDELIAAIKEAKGLSYETTESPRPVGQVADKPKTKTRSGLPTIPSPLDQEASSAESGDSEIELVSEDPMASEDALPSGYVSFGHVSGPPPSPASPSIQYSILREEREAELDSELIIESCDASSASEESPKREQDSPPMKPGALDAIREETGSRATEERAPSHQGPVEPDPMLSFAPAAALQSRPEPSSGDGASVPEPPRSQQQKPEEEAVSSSQSPTATEIPGPLGSGLMPPLPFFNKQKAIDLLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAYLELEITLSQEQIQKYTDCLQLYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAKIPGAKRHAE

Target ORF information:

RefSeq Version	XM_006515324.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus reticulon 1 (Rtn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515324.1

ORF Insert Sequence:

ATGGCAGCTG TTCCCGATGC CCTCGACCAC TCTCCCTCTT CAACACTAAA AGATGGGGAA 
GGGGCATGTT ACACATCCCT GATTTCTGAC GTCTGCTATC CACCTCGGGA GGACTCTGCA 
TACTTTACTG GAATTCTTCA GAAGGAAAAT GGTCACATCA CCACTTCCGA GAGCCCTGAG 
GAGCCGGAGA CCCCCGGACC CTCGTTACCA GAAGTGCCCG GGATGGAGCC ACAGGGCTTA 
TTGAGTTCTG ATTCTGGAAT AGAGATGACT CCTGCTGAGT CCACTGAGGT GAACAAGATC 
CTAGCTGACC CCCTAGACCA GATGAAGGCA GAAGCCTACA AATACATTGA CATAACCAGG 
CCACAGGAGG CAAAAGGCCA GGAGGAGCAA CACCCTGGGC TAGAAGATAA AGACTTGGAC 
TTTAAAGACA AGGACACTGA AGTCTCCACA AAAGCAGAAG GGGTTCGAGC ACCAAACCAG 
CCAGCTCCTG TGGAGGGGAA ACTCATCAAG GACCACTTAT TCGAAGAATC TACCTTTGCT 
CCTTACATAG ATGAGCTCTC TGATGAGCAG CACAGGGTGT CTCTGGTCAC TGCCCCTGTC 
AAGATCACAC TGACGGAGAT CGAACCTCCT CTTATGACTG CCACACAAGA GACAATCCCC 
GAGAAGCAAG ACCTGTGTCT GAAGCCAAGT CCTGACACAG TGCCCACTGT CACTGTCTCA 
GAGCCTGAAG ATGACAGCCC AGGATCTGTC ACGCCTCCAT CTTCTGGAAC AGAACCATCT 
GCTGCAGAGT CCCAGGGGAA AGGCAGCGTC TCCGAGGATG AGTTGATTGC TGCCATTAAA 
GAAGCAAAGG GGTTATCATA TGAAACCACC GAGAGTCCAA GGCCAGTGGG CCAGGTGGCC 
GACAAACCCA AAACCAAGAC CAGGTCTGGG CTGCCCACCA TCCCCAGTCC CCTCGACCAG 
GAGGCCAGCA GTGCCGAGTC CGGAGACTCA GAAATTGAGC TGGTGTCAGA GGACCCCATG 
GCCTCTGAGG ATGCCCTGCC CTCTGGCTAT GTGAGCTTCG GTCATGTGTC GGGCCCGCCC 
CCCTCTCCTG CCTCTCCATC CATCCAGTAC AGCATCCTGC GGGAGGAGCG TGAGGCCGAG 
CTGGACAGCG AGCTCATCAT CGAGTCCTGT GATGCCTCCT CAGCCTCGGA GGAGAGCCCC 
AAGCGCGAGC AGGACTCGCC CCCGATGAAA CCGGGTGCCC TGGACGCCAT CCGCGAGGAG 
ACAGGCTCTC GGGCCACCGA AGAACGAGCG CCCAGCCACC AAGGCCCTGT GGAGCCCGAC 
CCGATGCTCA GCTTTGCCCC CGCTGCCGCC CTCCAATCCC GCCCAGAGCC ATCGTCTGGA 
GATGGAGCCT CGGTCCCGGA GCCGCCCAGA TCACAGCAGC AGAAGCCTGA AGAGGAAGCT 
GTGAGTTCCA GTCAAAGTCC CACGGCCACC GAAATTCCTG GACCACTTGG TTCTGGCCTC 
ATGCCCCCTC TGCCTTTCTT CAACAAGCAA AAAGCTATTG ACCTTCTGTA CTGGCGGGAC 
ATCAAGCAGA CTGGGATTGT GTTCGGGAGC TTCCTGCTGC TGCTCTTCTC CCTGACCCAG 
TTCAGCGTTG TGAGCGTCGT TGCCTACCTG GCCCTGGCCG CCCTCTCTGC CACCATCAGC 
TTCCGCATCT ACAAGTCCGT TCTACAAGCT GTGCAGAAAA CAGATGAGGG TCACCCTTTC 
AAGGCCTACC TGGAGCTGGA GATCACCCTG TCCCAGGAGC AGATCCAGAA GTACACAGAC 
TGCCTGCAGC TGTATGTGAA CAGCACTCTG AAGGAGCTAC GGAGGCTTTT CCTGGTCCAG 
GACCTGGTGG ATTCCTTAAA ATTTGCAGTC CTCATGTGGC TCCTGACCTA CGTTGGCGCG 
CTCTTCAATG GCCTGACCCT GCTGCTTATG GCTGTGGTTT CGATGTTTAC TCTACCTGTT 
GTGTACGTTA AGCACCAGGC ACAAGTTGAC CAATATCTGG GACTTGTGAG GACTCACATA 
AACACCGTCG TGGCAAAGAT CCAGGCTAAA ATCCCCGGCG CCAAGAGGCA CGCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rtn1 ( XM_006515323.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515386.1 Mus musculus reticulon 1 (Rtn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47481
Clone ID Related Accession (Same CDS sequence)	XM_006515323.2 , XM_006515324.1 , XM_030246489.1 , XM_006515323.1 , XM_006515324.2
Accession Version	XM_006515323.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	reticulon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG TTCCCGATGC CCTCGACCAC TCTCCCTCTT CAACACTAAA AGATGGGGAA 
GGGGCATGTT ACACATCCCT GATTTCTGAC GTCTGCTATC CACCTCGGGA GGACTCTGCA 
TACTTTACTG GAATTCTTCA GAAGGAAAAT GGTCACATCA CCACTTCCGA GAGCCCTGAG 
GAGCCGGAGA CCCCCGGACC CTCGTTACCA GAAGTGCCCG GGATGGAGCC ACAGGGCTTA 
TTGAGTTCTG ATTCTGGAAT AGAGATGACT CCTGCTGAGT CCACTGAGGT GAACAAGATC 
CTAGCTGACC CCCTAGACCA GATGAAGGCA GAAGCCTACA AATACATTGA CATAACCAGG 
CCACAGGAGG CAAAAGGCCA GGAGGAGCAA CACCCTGGGC TAGAAGATAA AGACTTGGAC 
TTTAAAGACA AGGACACTGA AGTCTCCACA AAAGCAGAAG GGGTTCGAGC ACCAAACCAG 
CCAGCTCCTG TGGAGGGGAA ACTCATCAAG GACCACTTAT TCGAAGAATC TACCTTTGCT 
CCTTACATAG ATGAGCTCTC TGATGAGCAG CACAGGGTGT CTCTGGTCAC TGCCCCTGTC 
AAGATCACAC TGACGGAGAT CGAACCTCCT CTTATGACTG CCACACAAGA GACAATCCCC 
GAGAAGCAAG ACCTGTGTCT GAAGCCAAGT CCTGACACAG TGCCCACTGT CACTGTCTCA 
GAGCCTGAAG ATGACAGCCC AGGATCTGTC ACGCCTCCAT CTTCTGGAAC AGAACCATCT 
GCTGCAGAGT CCCAGGGGAA AGGCAGCGTC TCCGAGGATG AGTTGATTGC TGCCATTAAA 
GAAGCAAAGG GGTTATCATA TGAAACCACC GAGAGTCCAA GGCCAGTGGG CCAGGTGGCC 
GACAAACCCA AAACCAAGAC CAGGTCTGGG CTGCCCACCA TCCCCAGTCC CCTCGACCAG 
GAGGCCAGCA GTGCCGAGTC CGGAGACTCA GAAATTGAGC TGGTGTCAGA GGACCCCATG 
GCCTCTGAGG ATGCCCTGCC CTCTGGCTAT GTGAGCTTCG GTCATGTGTC GGGCCCGCCC 
CCCTCTCCTG CCTCTCCATC CATCCAGTAC AGCATCCTGC GGGAGGAGCG TGAGGCCGAG 
CTGGACAGCG AGCTCATCAT CGAGTCCTGT GATGCCTCCT CAGCCTCGGA GGAGAGCCCC 
AAGCGCGAGC AGGACTCGCC CCCGATGAAA CCGGGTGCCC TGGACGCCAT CCGCGAGGAG 
ACAGGCTCTC GGGCCACCGA AGAACGAGCG CCCAGCCACC AAGGCCCTGT GGAGCCCGAC 
CCGATGCTCA GCTTTGCCCC CGCTGCCGCC CTCCAATCCC GCCCAGAGCC ATCGTCTGGA 
GATGGAGCCT CGGTCCCGGA GCCGCCCAGA TCACAGCAGC AGAAGCCTGA AGAGGAAGCT 
GTGAGTTCCA GTCAAAGTCC CACGGCCACC GAAATTCCTG GACCACTTGG TTCTGGCCTC 
ATGCCCCCTC TGCCTTTCTT CAACAAGCAA AAAGCTATTG ACCTTCTGTA CTGGCGGGAC 
ATCAAGCAGA CTGGGATTGT GTTCGGGAGC TTCCTGCTGC TGCTCTTCTC CCTGACCCAG 
TTCAGCGTTG TGAGCGTCGT TGCCTACCTG GCCCTGGCCG CCCTCTCTGC CACCATCAGC 
TTCCGCATCT ACAAGTCCGT TCTACAAGCT GTGCAGAAAA CAGATGAGGG TCACCCTTTC 
AAGGCCTACC TGGAGCTGGA GATCACCCTG TCCCAGGAGC AGATCCAGAA GTACACAGAC 
TGCCTGCAGC TGTATGTGAA CAGCACTCTG AAGGAGCTAC GGAGGCTTTT CCTGGTCCAG 
GACCTGGTGG ATTCCTTAAA ATTTGCAGTC CTCATGTGGC TCCTGACCTA CGTTGGCGCG 
CTCTTCAATG GCCTGACCCT GCTGCTTATG GCTGTGGTTT CGATGTTTAC TCTACCTGTT 
GTGTACGTTA AGCACCAGGC ACAAGTTGAC CAATATCTGG GACTTGTGAG GACTCACATA 
AACACCGTCG TGGCAAAGAT CCAGGCTAAA ATCCCCGGCG CCAAGAGGCA CGCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515386.1
CDS	176..2275
Translation	MAAVPDALDHSPSSTLKDGEGACYTSLISDVCYPPREDSAYFTGILQKENGHITTSESPEEPETPGPSLPEVPGMEPQGLLSSDSGIEMTPAESTEVNKILADPLDQMKAEAYKYIDITRPQEAKGQEEQHPGLEDKDLDFKDKDTEVSTKAEGVRAPNQPAPVEGKLIKDHLFEESTFAPYIDELSDEQHRVSLVTAPVKITLTEIEPPLMTATQETIPEKQDLCLKPSPDTVPTVTVSEPEDDSPGSVTPPSSGTEPSAAESQGKGSVSEDELIAAIKEAKGLSYETTESPRPVGQVADKPKTKTRSGLPTIPSPLDQEASSAESGDSEIELVSEDPMASEDALPSGYVSFGHVSGPPPSPASPSIQYSILREEREAELDSELIIESCDASSASEESPKREQDSPPMKPGALDAIREETGSRATEERAPSHQGPVEPDPMLSFAPAAALQSRPEPSSGDGASVPEPPRSQQQKPEEEAVSSSQSPTATEIPGPLGSGLMPPLPFFNKQKAIDLLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAYLELEITLSQEQIQKYTDCLQLYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAKIPGAKRHAE

Target ORF information:

RefSeq Version	XM_006515323.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus reticulon 1 (Rtn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515323.1

ORF Insert Sequence:

ATGGCAGCTG TTCCCGATGC CCTCGACCAC TCTCCCTCTT CAACACTAAA AGATGGGGAA 
GGGGCATGTT ACACATCCCT GATTTCTGAC GTCTGCTATC CACCTCGGGA GGACTCTGCA 
TACTTTACTG GAATTCTTCA GAAGGAAAAT GGTCACATCA CCACTTCCGA GAGCCCTGAG 
GAGCCGGAGA CCCCCGGACC CTCGTTACCA GAAGTGCCCG GGATGGAGCC ACAGGGCTTA 
TTGAGTTCTG ATTCTGGAAT AGAGATGACT CCTGCTGAGT CCACTGAGGT GAACAAGATC 
CTAGCTGACC CCCTAGACCA GATGAAGGCA GAAGCCTACA AATACATTGA CATAACCAGG 
CCACAGGAGG CAAAAGGCCA GGAGGAGCAA CACCCTGGGC TAGAAGATAA AGACTTGGAC 
TTTAAAGACA AGGACACTGA AGTCTCCACA AAAGCAGAAG GGGTTCGAGC ACCAAACCAG 
CCAGCTCCTG TGGAGGGGAA ACTCATCAAG GACCACTTAT TCGAAGAATC TACCTTTGCT 
CCTTACATAG ATGAGCTCTC TGATGAGCAG CACAGGGTGT CTCTGGTCAC TGCCCCTGTC 
AAGATCACAC TGACGGAGAT CGAACCTCCT CTTATGACTG CCACACAAGA GACAATCCCC 
GAGAAGCAAG ACCTGTGTCT GAAGCCAAGT CCTGACACAG TGCCCACTGT CACTGTCTCA 
GAGCCTGAAG ATGACAGCCC AGGATCTGTC ACGCCTCCAT CTTCTGGAAC AGAACCATCT 
GCTGCAGAGT CCCAGGGGAA AGGCAGCGTC TCCGAGGATG AGTTGATTGC TGCCATTAAA 
GAAGCAAAGG GGTTATCATA TGAAACCACC GAGAGTCCAA GGCCAGTGGG CCAGGTGGCC 
GACAAACCCA AAACCAAGAC CAGGTCTGGG CTGCCCACCA TCCCCAGTCC CCTCGACCAG 
GAGGCCAGCA GTGCCGAGTC CGGAGACTCA GAAATTGAGC TGGTGTCAGA GGACCCCATG 
GCCTCTGAGG ATGCCCTGCC CTCTGGCTAT GTGAGCTTCG GTCATGTGTC GGGCCCGCCC 
CCCTCTCCTG CCTCTCCATC CATCCAGTAC AGCATCCTGC GGGAGGAGCG TGAGGCCGAG 
CTGGACAGCG AGCTCATCAT CGAGTCCTGT GATGCCTCCT CAGCCTCGGA GGAGAGCCCC 
AAGCGCGAGC AGGACTCGCC CCCGATGAAA CCGGGTGCCC TGGACGCCAT CCGCGAGGAG 
ACAGGCTCTC GGGCCACCGA AGAACGAGCG CCCAGCCACC AAGGCCCTGT GGAGCCCGAC 
CCGATGCTCA GCTTTGCCCC CGCTGCCGCC CTCCAATCCC GCCCAGAGCC ATCGTCTGGA 
GATGGAGCCT CGGTCCCGGA GCCGCCCAGA TCACAGCAGC AGAAGCCTGA AGAGGAAGCT 
GTGAGTTCCA GTCAAAGTCC CACGGCCACC GAAATTCCTG GACCACTTGG TTCTGGCCTC 
ATGCCCCCTC TGCCTTTCTT CAACAAGCAA AAAGCTATTG ACCTTCTGTA CTGGCGGGAC 
ATCAAGCAGA CTGGGATTGT GTTCGGGAGC TTCCTGCTGC TGCTCTTCTC CCTGACCCAG 
TTCAGCGTTG TGAGCGTCGT TGCCTACCTG GCCCTGGCCG CCCTCTCTGC CACCATCAGC 
TTCCGCATCT ACAAGTCCGT TCTACAAGCT GTGCAGAAAA CAGATGAGGG TCACCCTTTC 
AAGGCCTACC TGGAGCTGGA GATCACCCTG TCCCAGGAGC AGATCCAGAA GTACACAGAC 
TGCCTGCAGC TGTATGTGAA CAGCACTCTG AAGGAGCTAC GGAGGCTTTT CCTGGTCCAG 
GACCTGGTGG ATTCCTTAAA ATTTGCAGTC CTCATGTGGC TCCTGACCTA CGTTGGCGCG 
CTCTTCAATG GCCTGACCCT GCTGCTTATG GCTGTGGTTT CGATGTTTAC TCTACCTGTT 
GTGTACGTTA AGCACCAGGC ACAAGTTGAC CAATATCTGG GACTTGTGAG GACTCACATA 
AACACCGTCG TGGCAAAGAT CCAGGCTAAA ATCCCCGGCG CCAAGAGGCA CGCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rtn1 ( NM_001286448.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273377.1 Mus musculus reticulon 1 (Rtn1), transcript variant 3, mRNA.

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CloneID	OMu09310
Clone ID Related Accession (Same CDS sequence)	NM_001286448.1
Accession Version	NM_001286448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	reticulon-1 isoform A2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132364.1, BC058579.1, BC027091.1 and BY615773.1. Transcript Variant: This variant (3) lacks two in-frame exons in the central coding region, compared to variant 1. The encoded isoform (A2) is shorter, compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK001698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCAC CGCCGGATCT GCAAGATGAG CCGCTGTCTC TGGGTAGCCC CGGGTCCCAG 
TGGTTCGGGG GCCGGGGGGA TGGGGAAGAC GAAGCGACGG CGGTGATGGG GGCCAGGCCA 
GCGCAGCAGG ATGGGGAGCC CGCCTGGGGG TCGGGCGCTG GAGCTGGGGT AACGTCGTCG 
CGGGAACTGT GCTCCGGCCC CGCCCGGTCG CCTCCCGTCG CCATGGAAAC TGCATCCACA 
GCTATTGACC TTCTGTACTG GCGGGACATC AAGCAGACTG GGATTGTGTT CGGGAGCTTC 
CTGCTGCTGC TCTTCTCCCT GACCCAGTTC AGCGTTGTGA GCGTCGTTGC CTACCTGGCC 
CTGGCCGCCC TCTCTGCCAC CATCAGCTTC CGCATCTACA AGTCCGTTCT ACAAGCTGTG 
CAGAAAACAG ATGAGGGTCA CCCTTTCAAG GCCTACCTGG AGCTGGAGAT CACCCTGTCC 
CAGGAGCAGA TCCAGAAGTA CACAGACTGC CTGCAGCTGT ATGTGAACAG CACTCTGAAG 
GAGCTACGGA GGCTTTTCCT GGTCCAGGAC CTGGTGGATT CCTTAAAATT TGCAGTCCTC 
ATGTGGCTCC TGACCTACGT TGGCGCGCTC TTCAATGGCC TGACCCTGCT GCTTATGGCT 
GTGGTTTCGA TGTTTACTCT ACCTGTTGTG TACGTTAAGC ACCAGGCACA AGTTGACCAA 
TATCTGGGAC TTGTGAGGAC TCACATAAAC ACCGTCGTGG CAAAGATCCA GGCTAAAATC 
CCCGGCGCCA AGAGGCACGC TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273377.1
CDS	504..1310
Translation	MAAPPDLQDEPLSLGSPGSQWFGGRGDGEDEATAVMGARPAQQDGEPAWGSGAGAGVTSSRELCSGPARSPPVAMETASTAIDLLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAYLELEITLSQEQIQKYTDCLQLYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAKIPGAKRHAE

Target ORF information:

RefSeq Version	NM_001286448.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus reticulon 1 (Rtn1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286448.1

ORF Insert Sequence:

ATGGCCGCAC CGCCGGATCT GCAAGATGAG CCGCTGTCTC TGGGTAGCCC CGGGTCCCAG 
TGGTTCGGGG GCCGGGGGGA TGGGGAAGAC GAAGCGACGG CGGTGATGGG GGCCAGGCCA 
GCGCAGCAGG ATGGGGAGCC CGCCTGGGGG TCGGGCGCTG GAGCTGGGGT AACGTCGTCG 
CGGGAACTGT GCTCCGGCCC CGCCCGGTCG CCTCCCGTCG CCATGGAAAC TGCATCCACA 
GCTATTGACC TTCTGTACTG GCGGGACATC AAGCAGACTG GGATTGTGTT CGGGAGCTTC 
CTGCTGCTGC TCTTCTCCCT GACCCAGTTC AGCGTTGTGA GCGTCGTTGC CTACCTGGCC 
CTGGCCGCCC TCTCTGCCAC CATCAGCTTC CGCATCTACA AGTCCGTTCT ACAAGCTGTG 
CAGAAAACAG ATGAGGGTCA CCCTTTCAAG GCCTACCTGG AGCTGGAGAT CACCCTGTCC 
CAGGAGCAGA TCCAGAAGTA CACAGACTGC CTGCAGCTGT ATGTGAACAG CACTCTGAAG 
GAGCTACGGA GGCTTTTCCT GGTCCAGGAC CTGGTGGATT CCTTAAAATT TGCAGTCCTC 
ATGTGGCTCC TGACCTACGT TGGCGCGCTC TTCAATGGCC TGACCCTGCT GCTTATGGCT 
GTGGTTTCGA TGTTTACTCT ACCTGTTGTG TACGTTAAGC ACCAGGCACA AGTTGACCAA 
TATCTGGGAC TTGTGAGGAC TCACATAAAC ACCGTCGTGG CAAAGATCCA GGCTAAAATC 
CCCGGCGCCA AGAGGCACGC TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rtn1 ( NM_001007596.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001007597.1 Mus musculus reticulon 1 (Rtn1), transcript variant 2, mRNA.

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CloneID	OMu10814
Clone ID Related Accession (Same CDS sequence)	NM_001007596.2
Accession Version	NM_001007596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	reticulon-1 isoform C
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX241467.1, AK014358.1, BC027091.1 and BY615773.1. On Nov 6, 2013 this sequence version replaced NM_001007596.1. Transcript Variant: This variant (2) lacks part of the 5' coding region, uses an alternate 5'-terminal exon, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (C) has a shorter and distinct N-terminus, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK001693.1, SRR1660819.118529.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGCCA CTGCCGATTC CACCAAGATG GACTGTGTGT GGAGCAACTG GAAAAGTCAG 
GCTATTGACC TTCTGTACTG GCGGGACATC AAGCAGACTG GGATTGTGTT CGGGAGCTTC 
CTGCTGCTGC TCTTCTCCCT GACCCAGTTC AGCGTTGTGA GCGTCGTTGC CTACCTGGCC 
CTGGCCGCCC TCTCTGCCAC CATCAGCTTC CGCATCTACA AGTCCGTTCT ACAAGCTGTG 
CAGAAAACAG ATGAGGGTCA CCCTTTCAAG GCCTACCTGG AGCTGGAGAT CACCCTGTCC 
CAGGAGCAGA TCCAGAAGTA CACAGACTGC CTGCAGCTGT ATGTGAACAG CACTCTGAAG 
GAGCTACGGA GGCTTTTCCT GGTCCAGGAC CTGGTGGATT CCTTAAAATT TGCAGTCCTC 
ATGTGGCTCC TGACCTACGT TGGCGCGCTC TTCAATGGCC TGACCCTGCT GCTTATGGCT 
GTGGTTTCGA TGTTTACTCT ACCTGTTGTG TACGTTAAGC ACCAGGCACA AGTTGACCAA 
TATCTGGGAC TTGTGAGGAC TCACATAAAC ACCGTCGTGG CAAAGATCCA GGCTAAAATC 
CCCGGCGCCA AGAGGCACGC TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007597.1
CDS	139..765
Translation	MQATADSTKMDCVWSNWKSQAIDLLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAYLELEITLSQEQIQKYTDCLQLYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAKIPGAKRHAE

Target ORF information:

RefSeq Version	NM_001007596.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus reticulon 1 (Rtn1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007596.2

ORF Insert Sequence:

ATGCAGGCCA CTGCCGATTC CACCAAGATG GACTGTGTGT GGAGCAACTG GAAAAGTCAG 
GCTATTGACC TTCTGTACTG GCGGGACATC AAGCAGACTG GGATTGTGTT CGGGAGCTTC 
CTGCTGCTGC TCTTCTCCCT GACCCAGTTC AGCGTTGTGA GCGTCGTTGC CTACCTGGCC 
CTGGCCGCCC TCTCTGCCAC CATCAGCTTC CGCATCTACA AGTCCGTTCT ACAAGCTGTG 
CAGAAAACAG ATGAGGGTCA CCCTTTCAAG GCCTACCTGG AGCTGGAGAT CACCCTGTCC 
CAGGAGCAGA TCCAGAAGTA CACAGACTGC CTGCAGCTGT ATGTGAACAG CACTCTGAAG 
GAGCTACGGA GGCTTTTCCT GGTCCAGGAC CTGGTGGATT CCTTAAAATT TGCAGTCCTC 
ATGTGGCTCC TGACCTACGT TGGCGCGCTC TTCAATGGCC TGACCCTGCT GCTTATGGCT 
GTGGTTTCGA TGTTTACTCT ACCTGTTGTG TACGTTAAGC ACCAGGCACA AGTTGACCAA 
TATCTGGGAC TTGTGAGGAC TCACATAAAC ACCGTCGTGG CAAAGATCCA GGCTAAAATC 
CCCGGCGCCA AGAGGCACGC TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rtn1 ( NM_153457.7 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_703187.2 Mus musculus reticulon 1 (Rtn1), transcript variant 1, mRNA.

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CloneID	OMu02764
Clone ID Related Accession (Same CDS sequence)	NM_153457.7
Accession Version	NM_153457.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	reticulon-1 isoform A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132364.1, BC058579.1, BC027091.1 and BY615773.1. On Nov 6, 2013 this sequence version replaced NM_153457.6. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132364.1, BK001694.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCAC CGCCGGATCT GCAAGATGAG CCGCTGTCTC TGGGTAGCCC CGGGTCCCAG 
TGGTTCGGGG GCCGGGGGGA TGGGGAAGAC GAAGCGACGG CGGTGATGGG GGCCAGGCCA 
GCGCAGCAGG ATGGGGAGCC CGCCTGGGGG TCGGGCGCTG GAGCTGGGGT AACGTCGTCG 
CGGGAACTGT GCTCCGGCCC CGCCCGGTCG CCTCCCGTCG CCATGGAAAC TGCATCCACA 
GGTATGGCAG CTGTTCCCGA TGCCCTCGAC CACTCTCCCT CTTCAACACT AAAAGATGGG 
GAAGGGGCAT GTTACACATC CCTGATTTCT GACGTCTGCT ATCCACCTCG GGAGGACTCT 
GCATACTTTA CTGGAATTCT TCAGAAGGAA AATGGTCACA TCACCACTTC CGAGAGCCCT 
GAGGAGCCGG AGACCCCCGG ACCCTCGTTA CCAGAAGTGC CCGGGATGGA GCCACAGGGC 
TTATTGAGTT CTGATTCTGG AATAGAGATG ACTCCTGCTG AGTCCACTGA GGTGAACAAG 
ATCCTAGCTG ACCCCCTAGA CCAGATGAAG GCAGAAGCCT ACAAATACAT TGACATAACC 
AGGCCACAGG AGGCAAAAGG CCAGGAGGAG CAACACCCTG GGCTAGAAGA TAAAGACTTG 
GACTTTAAAG ACAAGGACAC TGAAGTCTCC ACAAAAGCAG AAGGGGTTCG AGCACCAAAC 
CAGCCAGCTC CTGTGGAGGG GAAACTCATC AAGGACCACT TATTCGAAGA ATCTACCTTT 
GCTCCTTACA TAGATGAGCT CTCTGATGAG CAGCACAGGG TGTCTCTGGT CACTGCCCCT 
GTCAAGATCA CACTGACGGA GATCGAACCT CCTCTTATGA CTGCCACACA AGAGACAATC 
CCCGAGAAGC AAGACCTGTG TCTGAAGCCA AGTCCTGACA CAGTGCCCAC TGTCACTGTC 
TCAGAGCCTG AAGATGACAG CCCAGGATCT GTCACGCCTC CATCTTCTGG AACAGAACCA 
TCTGCTGCAG AGTCCCAGGG GAAAGGCAGC GTCTCCGAGG ATGAGTTGAT TGCTGCCATT 
AAAGAAGCAA AGGGGTTATC ATATGAAACC ACCGAGAGTC CAAGGCCAGT GGGCCAGGTG 
GCCGACAAAC CCAAAACCAA GACCAGGTCT GGGCTGCCCA CCATCCCCAG TCCCCTCGAC 
CAGGAGGCCA GCAGTGCCGA GTCCGGAGAC TCAGAAATTG AGCTGGTGTC AGAGGACCCC 
ATGGCCTCTG AGGATGCCCT GCCCTCTGGC TATGTGAGCT TCGGTCATGT GTCGGGCCCG 
CCCCCCTCTC CTGCCTCTCC ATCCATCCAG TACAGCATCC TGCGGGAGGA GCGTGAGGCC 
GAGCTGGACA GCGAGCTCAT CATCGAGTCC TGTGATGCCT CCTCAGCCTC GGAGGAGAGC 
CCCAAGCGCG AGCAGGACTC GCCCCCGATG AAACCGGGTG CCCTGGACGC CATCCGCGAG 
GAGACAGGCT CTCGGGCCAC CGAAGAACGA GCGCCCAGCC ACCAAGGCCC TGTGGAGCCC 
GACCCGATGC TCAGCTTTGC CCCCGCTGCC GCCCTCCAAT CCCGCCCAGA GCCATCGTCT 
GGAGATGGAG CCTCGGTCCC GGAGCCGCCC AGATCACAGC AGCAGAAGCC TGAAGAGGAA 
GCTGTGAGTT CCAGTCAAAG TCCCACGGCC ACCGAAATTC CTGGACCACT TGGTTCTGGC 
CTCATGCCCC CTCTGCCTTT CTTCAACAAG CAAAAAGCTA TTGACCTTCT GTACTGGCGG 
GACATCAAGC AGACTGGGAT TGTGTTCGGG AGCTTCCTGC TGCTGCTCTT CTCCCTGACC 
CAGTTCAGCG TTGTGAGCGT CGTTGCCTAC CTGGCCCTGG CCGCCCTCTC TGCCACCATC 
AGCTTCCGCA TCTACAAGTC CGTTCTACAA GCTGTGCAGA AAACAGATGA GGGTCACCCT 
TTCAAGGCCT ACCTGGAGCT GGAGATCACC CTGTCCCAGG AGCAGATCCA GAAGTACACA 
GACTGCCTGC AGCTGTATGT GAACAGCACT CTGAAGGAGC TACGGAGGCT TTTCCTGGTC 
CAGGACCTGG TGGATTCCTT AAAATTTGCA GTCCTCATGT GGCTCCTGAC CTACGTTGGC 
GCGCTCTTCA ATGGCCTGAC CCTGCTGCTT ATGGCTGTGG TTTCGATGTT TACTCTACCT 
GTTGTGTACG TTAAGCACCA GGCACAAGTT GACCAATATC TGGGACTTGT GAGGACTCAC 
ATAAACACCG TCGTGGCAAA GATCCAGGCT AAAATCCCCG GCGCCAAGAG GCACGCTGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_703187.2
CDS	504..2846
Translation	MAAPPDLQDEPLSLGSPGSQWFGGRGDGEDEATAVMGARPAQQDGEPAWGSGAGAGVTSSRELCSGPARSPPVAMETASTGMAAVPDALDHSPSSTLKDGEGACYTSLISDVCYPPREDSAYFTGILQKENGHITTSESPEEPETPGPSLPEVPGMEPQGLLSSDSGIEMTPAESTEVNKILADPLDQMKAEAYKYIDITRPQEAKGQEEQHPGLEDKDLDFKDKDTEVSTKAEGVRAPNQPAPVEGKLIKDHLFEESTFAPYIDELSDEQHRVSLVTAPVKITLTEIEPPLMTATQETIPEKQDLCLKPSPDTVPTVTVSEPEDDSPGSVTPPSSGTEPSAAESQGKGSVSEDELIAAIKEAKGLSYETTESPRPVGQVADKPKTKTRSGLPTIPSPLDQEASSAESGDSEIELVSEDPMASEDALPSGYVSFGHVSGPPPSPASPSIQYSILREEREAELDSELIIESCDASSASEESPKREQDSPPMKPGALDAIREETGSRATEERAPSHQGPVEPDPMLSFAPAAALQSRPEPSSGDGASVPEPPRSQQQKPEEEAVSSSQSPTATEIPGPLGSGLMPPLPFFNKQKAIDLLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAYLELEITLSQEQIQKYTDCLQLYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAKIPGAKRHAE

Target ORF information:

RefSeq Version	NM_153457.7
Organism	Mus musculus(house mouse)
Definition	Mus musculus reticulon 1 (Rtn1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153457.7

ORF Insert Sequence:

ATGGCCGCAC CGCCGGATCT GCAAGATGAG CCGCTGTCTC TGGGTAGCCC CGGGTCCCAG 
TGGTTCGGGG GCCGGGGGGA TGGGGAAGAC GAAGCGACGG CGGTGATGGG GGCCAGGCCA 
GCGCAGCAGG ATGGGGAGCC CGCCTGGGGG TCGGGCGCTG GAGCTGGGGT AACGTCGTCG 
CGGGAACTGT GCTCCGGCCC CGCCCGGTCG CCTCCCGTCG CCATGGAAAC TGCATCCACA 
GGTATGGCAG CTGTTCCCGA TGCCCTCGAC CACTCTCCCT CTTCAACACT AAAAGATGGG 
GAAGGGGCAT GTTACACATC CCTGATTTCT GACGTCTGCT ATCCACCTCG GGAGGACTCT 
GCATACTTTA CTGGAATTCT TCAGAAGGAA AATGGTCACA TCACCACTTC CGAGAGCCCT 
GAGGAGCCGG AGACCCCCGG ACCCTCGTTA CCAGAAGTGC CCGGGATGGA GCCACAGGGC 
TTATTGAGTT CTGATTCTGG AATAGAGATG ACTCCTGCTG AGTCCACTGA GGTGAACAAG 
ATCCTAGCTG ACCCCCTAGA CCAGATGAAG GCAGAAGCCT ACAAATACAT TGACATAACC 
AGGCCACAGG AGGCAAAAGG CCAGGAGGAG CAACACCCTG GGCTAGAAGA TAAAGACTTG 
GACTTTAAAG ACAAGGACAC TGAAGTCTCC ACAAAAGCAG AAGGGGTTCG AGCACCAAAC 
CAGCCAGCTC CTGTGGAGGG GAAACTCATC AAGGACCACT TATTCGAAGA ATCTACCTTT 
GCTCCTTACA TAGATGAGCT CTCTGATGAG CAGCACAGGG TGTCTCTGGT CACTGCCCCT 
GTCAAGATCA CACTGACGGA GATCGAACCT CCTCTTATGA CTGCCACACA AGAGACAATC 
CCCGAGAAGC AAGACCTGTG TCTGAAGCCA AGTCCTGACA CAGTGCCCAC TGTCACTGTC 
TCAGAGCCTG AAGATGACAG CCCAGGATCT GTCACGCCTC CATCTTCTGG AACAGAACCA 
TCTGCTGCAG AGTCCCAGGG GAAAGGCAGC GTCTCCGAGG ATGAGTTGAT TGCTGCCATT 
AAAGAAGCAA AGGGGTTATC ATATGAAACC ACCGAGAGTC CAAGGCCAGT GGGCCAGGTG 
GCCGACAAAC CCAAAACCAA GACCAGGTCT GGGCTGCCCA CCATCCCCAG TCCCCTCGAC 
CAGGAGGCCA GCAGTGCCGA GTCCGGAGAC TCAGAAATTG AGCTGGTGTC AGAGGACCCC 
ATGGCCTCTG AGGATGCCCT GCCCTCTGGC TATGTGAGCT TCGGTCATGT GTCGGGCCCG 
CCCCCCTCTC CTGCCTCTCC ATCCATCCAG TACAGCATCC TGCGGGAGGA GCGTGAGGCC 
GAGCTGGACA GCGAGCTCAT CATCGAGTCC TGTGATGCCT CCTCAGCCTC GGAGGAGAGC 
CCCAAGCGCG AGCAGGACTC GCCCCCGATG AAACCGGGTG CCCTGGACGC CATCCGCGAG 
GAGACAGGCT CTCGGGCCAC CGAAGAACGA GCGCCCAGCC ACCAAGGCCC TGTGGAGCCC 
GACCCGATGC TCAGCTTTGC CCCCGCTGCC GCCCTCCAAT CCCGCCCAGA GCCATCGTCT 
GGAGATGGAG CCTCGGTCCC GGAGCCGCCC AGATCACAGC AGCAGAAGCC TGAAGAGGAA 
GCTGTGAGTT CCAGTCAAAG TCCCACGGCC ACCGAAATTC CTGGACCACT TGGTTCTGGC 
CTCATGCCCC CTCTGCCTTT CTTCAACAAG CAAAAAGCTA TTGACCTTCT GTACTGGCGG 
GACATCAAGC AGACTGGGAT TGTGTTCGGG AGCTTCCTGC TGCTGCTCTT CTCCCTGACC 
CAGTTCAGCG TTGTGAGCGT CGTTGCCTAC CTGGCCCTGG CCGCCCTCTC TGCCACCATC 
AGCTTCCGCA TCTACAAGTC CGTTCTACAA GCTGTGCAGA AAACAGATGA GGGTCACCCT 
TTCAAGGCCT ACCTGGAGCT GGAGATCACC CTGTCCCAGG AGCAGATCCA GAAGTACACA 
GACTGCCTGC AGCTGTATGT GAACAGCACT CTGAAGGAGC TACGGAGGCT TTTCCTGGTC 
CAGGACCTGG TGGATTCCTT AAAATTTGCA GTCCTCATGT GGCTCCTGAC CTACGTTGGC 
GCGCTCTTCA ATGGCCTGAC CCTGCTGCTT ATGGCTGTGG TTTCGATGTT TACTCTACCT 
GTTGTGTACG TTAAGCACCA GGCACAAGTT GACCAATATC TGGGACTTGT GAGGACTCAC 
ATAAACACCG TCGTGGCAAA GATCCAGGCT AAAATCCCCG GCGCCAAGAG GCACGCTGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rtn1 ( XM_006515323.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515386.1 Mus musculus reticulon 1 (Rtn1), transcript variant X2, mRNA.

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CloneID	OMu47481
Clone ID Related Accession (Same CDS sequence)	XM_006515323.2 , XM_006515324.1 , XM_030246489.1 , XM_006515323.1 , XM_006515324.2
Accession Version	XM_006515323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	reticulon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG TTCCCGATGC CCTCGACCAC TCTCCCTCTT CAACACTAAA AGATGGGGAA 
GGGGCATGTT ACACATCCCT GATTTCTGAC GTCTGCTATC CACCTCGGGA GGACTCTGCA 
TACTTTACTG GAATTCTTCA GAAGGAAAAT GGTCACATCA CCACTTCCGA GAGCCCTGAG 
GAGCCGGAGA CCCCCGGACC CTCGTTACCA GAAGTGCCCG GGATGGAGCC ACAGGGCTTA 
TTGAGTTCTG ATTCTGGAAT AGAGATGACT CCTGCTGAGT CCACTGAGGT GAACAAGATC 
CTAGCTGACC CCCTAGACCA GATGAAGGCA GAAGCCTACA AATACATTGA CATAACCAGG 
CCACAGGAGG CAAAAGGCCA GGAGGAGCAA CACCCTGGGC TAGAAGATAA AGACTTGGAC 
TTTAAAGACA AGGACACTGA AGTCTCCACA AAAGCAGAAG GGGTTCGAGC ACCAAACCAG 
CCAGCTCCTG TGGAGGGGAA ACTCATCAAG GACCACTTAT TCGAAGAATC TACCTTTGCT 
CCTTACATAG ATGAGCTCTC TGATGAGCAG CACAGGGTGT CTCTGGTCAC TGCCCCTGTC 
AAGATCACAC TGACGGAGAT CGAACCTCCT CTTATGACTG CCACACAAGA GACAATCCCC 
GAGAAGCAAG ACCTGTGTCT GAAGCCAAGT CCTGACACAG TGCCCACTGT CACTGTCTCA 
GAGCCTGAAG ATGACAGCCC AGGATCTGTC ACGCCTCCAT CTTCTGGAAC AGAACCATCT 
GCTGCAGAGT CCCAGGGGAA AGGCAGCGTC TCCGAGGATG AGTTGATTGC TGCCATTAAA 
GAAGCAAAGG GGTTATCATA TGAAACCACC GAGAGTCCAA GGCCAGTGGG CCAGGTGGCC 
GACAAACCCA AAACCAAGAC CAGGTCTGGG CTGCCCACCA TCCCCAGTCC CCTCGACCAG 
GAGGCCAGCA GTGCCGAGTC CGGAGACTCA GAAATTGAGC TGGTGTCAGA GGACCCCATG 
GCCTCTGAGG ATGCCCTGCC CTCTGGCTAT GTGAGCTTCG GTCATGTGTC GGGCCCGCCC 
CCCTCTCCTG CCTCTCCATC CATCCAGTAC AGCATCCTGC GGGAGGAGCG TGAGGCCGAG 
CTGGACAGCG AGCTCATCAT CGAGTCCTGT GATGCCTCCT CAGCCTCGGA GGAGAGCCCC 
AAGCGCGAGC AGGACTCGCC CCCGATGAAA CCGGGTGCCC TGGACGCCAT CCGCGAGGAG 
ACAGGCTCTC GGGCCACCGA AGAACGAGCG CCCAGCCACC AAGGCCCTGT GGAGCCCGAC 
CCGATGCTCA GCTTTGCCCC CGCTGCCGCC CTCCAATCCC GCCCAGAGCC ATCGTCTGGA 
GATGGAGCCT CGGTCCCGGA GCCGCCCAGA TCACAGCAGC AGAAGCCTGA AGAGGAAGCT 
GTGAGTTCCA GTCAAAGTCC CACGGCCACC GAAATTCCTG GACCACTTGG TTCTGGCCTC 
ATGCCCCCTC TGCCTTTCTT CAACAAGCAA AAAGCTATTG ACCTTCTGTA CTGGCGGGAC 
ATCAAGCAGA CTGGGATTGT GTTCGGGAGC TTCCTGCTGC TGCTCTTCTC CCTGACCCAG 
TTCAGCGTTG TGAGCGTCGT TGCCTACCTG GCCCTGGCCG CCCTCTCTGC CACCATCAGC 
TTCCGCATCT ACAAGTCCGT TCTACAAGCT GTGCAGAAAA CAGATGAGGG TCACCCTTTC 
AAGGCCTACC TGGAGCTGGA GATCACCCTG TCCCAGGAGC AGATCCAGAA GTACACAGAC 
TGCCTGCAGC TGTATGTGAA CAGCACTCTG AAGGAGCTAC GGAGGCTTTT CCTGGTCCAG 
GACCTGGTGG ATTCCTTAAA ATTTGCAGTC CTCATGTGGC TCCTGACCTA CGTTGGCGCG 
CTCTTCAATG GCCTGACCCT GCTGCTTATG GCTGTGGTTT CGATGTTTAC TCTACCTGTT 
GTGTACGTTA AGCACCAGGC ACAAGTTGAC CAATATCTGG GACTTGTGAG GACTCACATA 
AACACCGTCG TGGCAAAGAT CCAGGCTAAA ATCCCCGGCG CCAAGAGGCA CGCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515386.1
CDS	215..2314
Translation	MAAVPDALDHSPSSTLKDGEGACYTSLISDVCYPPREDSAYFTGILQKENGHITTSESPEEPETPGPSLPEVPGMEPQGLLSSDSGIEMTPAESTEVNKILADPLDQMKAEAYKYIDITRPQEAKGQEEQHPGLEDKDLDFKDKDTEVSTKAEGVRAPNQPAPVEGKLIKDHLFEESTFAPYIDELSDEQHRVSLVTAPVKITLTEIEPPLMTATQETIPEKQDLCLKPSPDTVPTVTVSEPEDDSPGSVTPPSSGTEPSAAESQGKGSVSEDELIAAIKEAKGLSYETTESPRPVGQVADKPKTKTRSGLPTIPSPLDQEASSAESGDSEIELVSEDPMASEDALPSGYVSFGHVSGPPPSPASPSIQYSILREEREAELDSELIIESCDASSASEESPKREQDSPPMKPGALDAIREETGSRATEERAPSHQGPVEPDPMLSFAPAAALQSRPEPSSGDGASVPEPPRSQQQKPEEEAVSSSQSPTATEIPGPLGSGLMPPLPFFNKQKAIDLLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAYLELEITLSQEQIQKYTDCLQLYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAKIPGAKRHAE

Target ORF information:

RefSeq Version	XM_006515323.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus reticulon 1 (Rtn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515323.2

ORF Insert Sequence:

ATGGCAGCTG TTCCCGATGC CCTCGACCAC TCTCCCTCTT CAACACTAAA AGATGGGGAA 
GGGGCATGTT ACACATCCCT GATTTCTGAC GTCTGCTATC CACCTCGGGA GGACTCTGCA 
TACTTTACTG GAATTCTTCA GAAGGAAAAT GGTCACATCA CCACTTCCGA GAGCCCTGAG 
GAGCCGGAGA CCCCCGGACC CTCGTTACCA GAAGTGCCCG GGATGGAGCC ACAGGGCTTA 
TTGAGTTCTG ATTCTGGAAT AGAGATGACT CCTGCTGAGT CCACTGAGGT GAACAAGATC 
CTAGCTGACC CCCTAGACCA GATGAAGGCA GAAGCCTACA AATACATTGA CATAACCAGG 
CCACAGGAGG CAAAAGGCCA GGAGGAGCAA CACCCTGGGC TAGAAGATAA AGACTTGGAC 
TTTAAAGACA AGGACACTGA AGTCTCCACA AAAGCAGAAG GGGTTCGAGC ACCAAACCAG 
CCAGCTCCTG TGGAGGGGAA ACTCATCAAG GACCACTTAT TCGAAGAATC TACCTTTGCT 
CCTTACATAG ATGAGCTCTC TGATGAGCAG CACAGGGTGT CTCTGGTCAC TGCCCCTGTC 
AAGATCACAC TGACGGAGAT CGAACCTCCT CTTATGACTG CCACACAAGA GACAATCCCC 
GAGAAGCAAG ACCTGTGTCT GAAGCCAAGT CCTGACACAG TGCCCACTGT CACTGTCTCA 
GAGCCTGAAG ATGACAGCCC AGGATCTGTC ACGCCTCCAT CTTCTGGAAC AGAACCATCT 
GCTGCAGAGT CCCAGGGGAA AGGCAGCGTC TCCGAGGATG AGTTGATTGC TGCCATTAAA 
GAAGCAAAGG GGTTATCATA TGAAACCACC GAGAGTCCAA GGCCAGTGGG CCAGGTGGCC 
GACAAACCCA AAACCAAGAC CAGGTCTGGG CTGCCCACCA TCCCCAGTCC CCTCGACCAG 
GAGGCCAGCA GTGCCGAGTC CGGAGACTCA GAAATTGAGC TGGTGTCAGA GGACCCCATG 
GCCTCTGAGG ATGCCCTGCC CTCTGGCTAT GTGAGCTTCG GTCATGTGTC GGGCCCGCCC 
CCCTCTCCTG CCTCTCCATC CATCCAGTAC AGCATCCTGC GGGAGGAGCG TGAGGCCGAG 
CTGGACAGCG AGCTCATCAT CGAGTCCTGT GATGCCTCCT CAGCCTCGGA GGAGAGCCCC 
AAGCGCGAGC AGGACTCGCC CCCGATGAAA CCGGGTGCCC TGGACGCCAT CCGCGAGGAG 
ACAGGCTCTC GGGCCACCGA AGAACGAGCG CCCAGCCACC AAGGCCCTGT GGAGCCCGAC 
CCGATGCTCA GCTTTGCCCC CGCTGCCGCC CTCCAATCCC GCCCAGAGCC ATCGTCTGGA 
GATGGAGCCT CGGTCCCGGA GCCGCCCAGA TCACAGCAGC AGAAGCCTGA AGAGGAAGCT 
GTGAGTTCCA GTCAAAGTCC CACGGCCACC GAAATTCCTG GACCACTTGG TTCTGGCCTC 
ATGCCCCCTC TGCCTTTCTT CAACAAGCAA AAAGCTATTG ACCTTCTGTA CTGGCGGGAC 
ATCAAGCAGA CTGGGATTGT GTTCGGGAGC TTCCTGCTGC TGCTCTTCTC CCTGACCCAG 
TTCAGCGTTG TGAGCGTCGT TGCCTACCTG GCCCTGGCCG CCCTCTCTGC CACCATCAGC 
TTCCGCATCT ACAAGTCCGT TCTACAAGCT GTGCAGAAAA CAGATGAGGG TCACCCTTTC 
AAGGCCTACC TGGAGCTGGA GATCACCCTG TCCCAGGAGC AGATCCAGAA GTACACAGAC 
TGCCTGCAGC TGTATGTGAA CAGCACTCTG AAGGAGCTAC GGAGGCTTTT CCTGGTCCAG 
GACCTGGTGG ATTCCTTAAA ATTTGCAGTC CTCATGTGGC TCCTGACCTA CGTTGGCGCG 
CTCTTCAATG GCCTGACCCT GCTGCTTATG GCTGTGGTTT CGATGTTTAC TCTACCTGTT 
GTGTACGTTA AGCACCAGGC ACAAGTTGAC CAATATCTGG GACTTGTGAG GACTCACATA 
AACACCGTCG TGGCAAAGAT CCAGGCTAAA ATCCCCGGCG CCAAGAGGCA CGCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rtn1 ( XM_030246489.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102349.1 Mus musculus reticulon 1 (Rtn1), transcript variant X1, mRNA.

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CloneID	OMu47481
Clone ID Related Accession (Same CDS sequence)	XM_006515323.2 , XM_006515324.1 , XM_030246489.1 , XM_006515323.1 , XM_006515324.2
Accession Version	XM_030246489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	reticulon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG TTCCCGATGC CCTCGACCAC TCTCCCTCTT CAACACTAAA AGATGGGGAA 
GGGGCATGTT ACACATCCCT GATTTCTGAC GTCTGCTATC CACCTCGGGA GGACTCTGCA 
TACTTTACTG GAATTCTTCA GAAGGAAAAT GGTCACATCA CCACTTCCGA GAGCCCTGAG 
GAGCCGGAGA CCCCCGGACC CTCGTTACCA GAAGTGCCCG GGATGGAGCC ACAGGGCTTA 
TTGAGTTCTG ATTCTGGAAT AGAGATGACT CCTGCTGAGT CCACTGAGGT GAACAAGATC 
CTAGCTGACC CCCTAGACCA GATGAAGGCA GAAGCCTACA AATACATTGA CATAACCAGG 
CCACAGGAGG CAAAAGGCCA GGAGGAGCAA CACCCTGGGC TAGAAGATAA AGACTTGGAC 
TTTAAAGACA AGGACACTGA AGTCTCCACA AAAGCAGAAG GGGTTCGAGC ACCAAACCAG 
CCAGCTCCTG TGGAGGGGAA ACTCATCAAG GACCACTTAT TCGAAGAATC TACCTTTGCT 
CCTTACATAG ATGAGCTCTC TGATGAGCAG CACAGGGTGT CTCTGGTCAC TGCCCCTGTC 
AAGATCACAC TGACGGAGAT CGAACCTCCT CTTATGACTG CCACACAAGA GACAATCCCC 
GAGAAGCAAG ACCTGTGTCT GAAGCCAAGT CCTGACACAG TGCCCACTGT CACTGTCTCA 
GAGCCTGAAG ATGACAGCCC AGGATCTGTC ACGCCTCCAT CTTCTGGAAC AGAACCATCT 
GCTGCAGAGT CCCAGGGGAA AGGCAGCGTC TCCGAGGATG AGTTGATTGC TGCCATTAAA 
GAAGCAAAGG GGTTATCATA TGAAACCACC GAGAGTCCAA GGCCAGTGGG CCAGGTGGCC 
GACAAACCCA AAACCAAGAC CAGGTCTGGG CTGCCCACCA TCCCCAGTCC CCTCGACCAG 
GAGGCCAGCA GTGCCGAGTC CGGAGACTCA GAAATTGAGC TGGTGTCAGA GGACCCCATG 
GCCTCTGAGG ATGCCCTGCC CTCTGGCTAT GTGAGCTTCG GTCATGTGTC GGGCCCGCCC 
CCCTCTCCTG CCTCTCCATC CATCCAGTAC AGCATCCTGC GGGAGGAGCG TGAGGCCGAG 
CTGGACAGCG AGCTCATCAT CGAGTCCTGT GATGCCTCCT CAGCCTCGGA GGAGAGCCCC 
AAGCGCGAGC AGGACTCGCC CCCGATGAAA CCGGGTGCCC TGGACGCCAT CCGCGAGGAG 
ACAGGCTCTC GGGCCACCGA AGAACGAGCG CCCAGCCACC AAGGCCCTGT GGAGCCCGAC 
CCGATGCTCA GCTTTGCCCC CGCTGCCGCC CTCCAATCCC GCCCAGAGCC ATCGTCTGGA 
GATGGAGCCT CGGTCCCGGA GCCGCCCAGA TCACAGCAGC AGAAGCCTGA AGAGGAAGCT 
GTGAGTTCCA GTCAAAGTCC CACGGCCACC GAAATTCCTG GACCACTTGG TTCTGGCCTC 
ATGCCCCCTC TGCCTTTCTT CAACAAGCAA AAAGCTATTG ACCTTCTGTA CTGGCGGGAC 
ATCAAGCAGA CTGGGATTGT GTTCGGGAGC TTCCTGCTGC TGCTCTTCTC CCTGACCCAG 
TTCAGCGTTG TGAGCGTCGT TGCCTACCTG GCCCTGGCCG CCCTCTCTGC CACCATCAGC 
TTCCGCATCT ACAAGTCCGT TCTACAAGCT GTGCAGAAAA CAGATGAGGG TCACCCTTTC 
AAGGCCTACC TGGAGCTGGA GATCACCCTG TCCCAGGAGC AGATCCAGAA GTACACAGAC 
TGCCTGCAGC TGTATGTGAA CAGCACTCTG AAGGAGCTAC GGAGGCTTTT CCTGGTCCAG 
GACCTGGTGG ATTCCTTAAA ATTTGCAGTC CTCATGTGGC TCCTGACCTA CGTTGGCGCG 
CTCTTCAATG GCCTGACCCT GCTGCTTATG GCTGTGGTTT CGATGTTTAC TCTACCTGTT 
GTGTACGTTA AGCACCAGGC ACAAGTTGAC CAATATCTGG GACTTGTGAG GACTCACATA 
AACACCGTCG TGGCAAAGAT CCAGGCTAAA ATCCCCGGCG CCAAGAGGCA CGCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102349.1
CDS	134..2233
Translation	MAAVPDALDHSPSSTLKDGEGACYTSLISDVCYPPREDSAYFTGILQKENGHITTSESPEEPETPGPSLPEVPGMEPQGLLSSDSGIEMTPAESTEVNKILADPLDQMKAEAYKYIDITRPQEAKGQEEQHPGLEDKDLDFKDKDTEVSTKAEGVRAPNQPAPVEGKLIKDHLFEESTFAPYIDELSDEQHRVSLVTAPVKITLTEIEPPLMTATQETIPEKQDLCLKPSPDTVPTVTVSEPEDDSPGSVTPPSSGTEPSAAESQGKGSVSEDELIAAIKEAKGLSYETTESPRPVGQVADKPKTKTRSGLPTIPSPLDQEASSAESGDSEIELVSEDPMASEDALPSGYVSFGHVSGPPPSPASPSIQYSILREEREAELDSELIIESCDASSASEESPKREQDSPPMKPGALDAIREETGSRATEERAPSHQGPVEPDPMLSFAPAAALQSRPEPSSGDGASVPEPPRSQQQKPEEEAVSSSQSPTATEIPGPLGSGLMPPLPFFNKQKAIDLLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAYLELEITLSQEQIQKYTDCLQLYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAKIPGAKRHAE

Target ORF information:

RefSeq Version	XM_030246489.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus reticulon 1 (Rtn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246489.1

ORF Insert Sequence:

ATGGCAGCTG TTCCCGATGC CCTCGACCAC TCTCCCTCTT CAACACTAAA AGATGGGGAA 
GGGGCATGTT ACACATCCCT GATTTCTGAC GTCTGCTATC CACCTCGGGA GGACTCTGCA 
TACTTTACTG GAATTCTTCA GAAGGAAAAT GGTCACATCA CCACTTCCGA GAGCCCTGAG 
GAGCCGGAGA CCCCCGGACC CTCGTTACCA GAAGTGCCCG GGATGGAGCC ACAGGGCTTA 
TTGAGTTCTG ATTCTGGAAT AGAGATGACT CCTGCTGAGT CCACTGAGGT GAACAAGATC 
CTAGCTGACC CCCTAGACCA GATGAAGGCA GAAGCCTACA AATACATTGA CATAACCAGG 
CCACAGGAGG CAAAAGGCCA GGAGGAGCAA CACCCTGGGC TAGAAGATAA AGACTTGGAC 
TTTAAAGACA AGGACACTGA AGTCTCCACA AAAGCAGAAG GGGTTCGAGC ACCAAACCAG 
CCAGCTCCTG TGGAGGGGAA ACTCATCAAG GACCACTTAT TCGAAGAATC TACCTTTGCT 
CCTTACATAG ATGAGCTCTC TGATGAGCAG CACAGGGTGT CTCTGGTCAC TGCCCCTGTC 
AAGATCACAC TGACGGAGAT CGAACCTCCT CTTATGACTG CCACACAAGA GACAATCCCC 
GAGAAGCAAG ACCTGTGTCT GAAGCCAAGT CCTGACACAG TGCCCACTGT CACTGTCTCA 
GAGCCTGAAG ATGACAGCCC AGGATCTGTC ACGCCTCCAT CTTCTGGAAC AGAACCATCT 
GCTGCAGAGT CCCAGGGGAA AGGCAGCGTC TCCGAGGATG AGTTGATTGC TGCCATTAAA 
GAAGCAAAGG GGTTATCATA TGAAACCACC GAGAGTCCAA GGCCAGTGGG CCAGGTGGCC 
GACAAACCCA AAACCAAGAC CAGGTCTGGG CTGCCCACCA TCCCCAGTCC CCTCGACCAG 
GAGGCCAGCA GTGCCGAGTC CGGAGACTCA GAAATTGAGC TGGTGTCAGA GGACCCCATG 
GCCTCTGAGG ATGCCCTGCC CTCTGGCTAT GTGAGCTTCG GTCATGTGTC GGGCCCGCCC 
CCCTCTCCTG CCTCTCCATC CATCCAGTAC AGCATCCTGC GGGAGGAGCG TGAGGCCGAG 
CTGGACAGCG AGCTCATCAT CGAGTCCTGT GATGCCTCCT CAGCCTCGGA GGAGAGCCCC 
AAGCGCGAGC AGGACTCGCC CCCGATGAAA CCGGGTGCCC TGGACGCCAT CCGCGAGGAG 
ACAGGCTCTC GGGCCACCGA AGAACGAGCG CCCAGCCACC AAGGCCCTGT GGAGCCCGAC 
CCGATGCTCA GCTTTGCCCC CGCTGCCGCC CTCCAATCCC GCCCAGAGCC ATCGTCTGGA 
GATGGAGCCT CGGTCCCGGA GCCGCCCAGA TCACAGCAGC AGAAGCCTGA AGAGGAAGCT 
GTGAGTTCCA GTCAAAGTCC CACGGCCACC GAAATTCCTG GACCACTTGG TTCTGGCCTC 
ATGCCCCCTC TGCCTTTCTT CAACAAGCAA AAAGCTATTG ACCTTCTGTA CTGGCGGGAC 
ATCAAGCAGA CTGGGATTGT GTTCGGGAGC TTCCTGCTGC TGCTCTTCTC CCTGACCCAG 
TTCAGCGTTG TGAGCGTCGT TGCCTACCTG GCCCTGGCCG CCCTCTCTGC CACCATCAGC 
TTCCGCATCT ACAAGTCCGT TCTACAAGCT GTGCAGAAAA CAGATGAGGG TCACCCTTTC 
AAGGCCTACC TGGAGCTGGA GATCACCCTG TCCCAGGAGC AGATCCAGAA GTACACAGAC 
TGCCTGCAGC TGTATGTGAA CAGCACTCTG AAGGAGCTAC GGAGGCTTTT CCTGGTCCAG 
GACCTGGTGG ATTCCTTAAA ATTTGCAGTC CTCATGTGGC TCCTGACCTA CGTTGGCGCG 
CTCTTCAATG GCCTGACCCT GCTGCTTATG GCTGTGGTTT CGATGTTTAC TCTACCTGTT 
GTGTACGTTA AGCACCAGGC ACAAGTTGAC CAATATCTGG GACTTGTGAG GACTCACATA 
AACACCGTCG TGGCAAAGAT CCAGGCTAAA ATCCCCGGCG CCAAGAGGCA CGCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rtn1 ( XM_006515324.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515387.1 Mus musculus reticulon 1 (Rtn1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu47481
Clone ID Related Accession (Same CDS sequence)	XM_006515323.2 , XM_006515324.1 , XM_030246489.1 , XM_006515323.1 , XM_006515324.2
Accession Version	XM_006515324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	reticulon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG TTCCCGATGC CCTCGACCAC TCTCCCTCTT CAACACTAAA AGATGGGGAA 
GGGGCATGTT ACACATCCCT GATTTCTGAC GTCTGCTATC CACCTCGGGA GGACTCTGCA 
TACTTTACTG GAATTCTTCA GAAGGAAAAT GGTCACATCA CCACTTCCGA GAGCCCTGAG 
GAGCCGGAGA CCCCCGGACC CTCGTTACCA GAAGTGCCCG GGATGGAGCC ACAGGGCTTA 
TTGAGTTCTG ATTCTGGAAT AGAGATGACT CCTGCTGAGT CCACTGAGGT GAACAAGATC 
CTAGCTGACC CCCTAGACCA GATGAAGGCA GAAGCCTACA AATACATTGA CATAACCAGG 
CCACAGGAGG CAAAAGGCCA GGAGGAGCAA CACCCTGGGC TAGAAGATAA AGACTTGGAC 
TTTAAAGACA AGGACACTGA AGTCTCCACA AAAGCAGAAG GGGTTCGAGC ACCAAACCAG 
CCAGCTCCTG TGGAGGGGAA ACTCATCAAG GACCACTTAT TCGAAGAATC TACCTTTGCT 
CCTTACATAG ATGAGCTCTC TGATGAGCAG CACAGGGTGT CTCTGGTCAC TGCCCCTGTC 
AAGATCACAC TGACGGAGAT CGAACCTCCT CTTATGACTG CCACACAAGA GACAATCCCC 
GAGAAGCAAG ACCTGTGTCT GAAGCCAAGT CCTGACACAG TGCCCACTGT CACTGTCTCA 
GAGCCTGAAG ATGACAGCCC AGGATCTGTC ACGCCTCCAT CTTCTGGAAC AGAACCATCT 
GCTGCAGAGT CCCAGGGGAA AGGCAGCGTC TCCGAGGATG AGTTGATTGC TGCCATTAAA 
GAAGCAAAGG GGTTATCATA TGAAACCACC GAGAGTCCAA GGCCAGTGGG CCAGGTGGCC 
GACAAACCCA AAACCAAGAC CAGGTCTGGG CTGCCCACCA TCCCCAGTCC CCTCGACCAG 
GAGGCCAGCA GTGCCGAGTC CGGAGACTCA GAAATTGAGC TGGTGTCAGA GGACCCCATG 
GCCTCTGAGG ATGCCCTGCC CTCTGGCTAT GTGAGCTTCG GTCATGTGTC GGGCCCGCCC 
CCCTCTCCTG CCTCTCCATC CATCCAGTAC AGCATCCTGC GGGAGGAGCG TGAGGCCGAG 
CTGGACAGCG AGCTCATCAT CGAGTCCTGT GATGCCTCCT CAGCCTCGGA GGAGAGCCCC 
AAGCGCGAGC AGGACTCGCC CCCGATGAAA CCGGGTGCCC TGGACGCCAT CCGCGAGGAG 
ACAGGCTCTC GGGCCACCGA AGAACGAGCG CCCAGCCACC AAGGCCCTGT GGAGCCCGAC 
CCGATGCTCA GCTTTGCCCC CGCTGCCGCC CTCCAATCCC GCCCAGAGCC ATCGTCTGGA 
GATGGAGCCT CGGTCCCGGA GCCGCCCAGA TCACAGCAGC AGAAGCCTGA AGAGGAAGCT 
GTGAGTTCCA GTCAAAGTCC CACGGCCACC GAAATTCCTG GACCACTTGG TTCTGGCCTC 
ATGCCCCCTC TGCCTTTCTT CAACAAGCAA AAAGCTATTG ACCTTCTGTA CTGGCGGGAC 
ATCAAGCAGA CTGGGATTGT GTTCGGGAGC TTCCTGCTGC TGCTCTTCTC CCTGACCCAG 
TTCAGCGTTG TGAGCGTCGT TGCCTACCTG GCCCTGGCCG CCCTCTCTGC CACCATCAGC 
TTCCGCATCT ACAAGTCCGT TCTACAAGCT GTGCAGAAAA CAGATGAGGG TCACCCTTTC 
AAGGCCTACC TGGAGCTGGA GATCACCCTG TCCCAGGAGC AGATCCAGAA GTACACAGAC 
TGCCTGCAGC TGTATGTGAA CAGCACTCTG AAGGAGCTAC GGAGGCTTTT CCTGGTCCAG 
GACCTGGTGG ATTCCTTAAA ATTTGCAGTC CTCATGTGGC TCCTGACCTA CGTTGGCGCG 
CTCTTCAATG GCCTGACCCT GCTGCTTATG GCTGTGGTTT CGATGTTTAC TCTACCTGTT 
GTGTACGTTA AGCACCAGGC ACAAGTTGAC CAATATCTGG GACTTGTGAG GACTCACATA 
AACACCGTCG TGGCAAAGAT CCAGGCTAAA ATCCCCGGCG CCAAGAGGCA CGCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515387.1
CDS	147..2246
Translation	MAAVPDALDHSPSSTLKDGEGACYTSLISDVCYPPREDSAYFTGILQKENGHITTSESPEEPETPGPSLPEVPGMEPQGLLSSDSGIEMTPAESTEVNKILADPLDQMKAEAYKYIDITRPQEAKGQEEQHPGLEDKDLDFKDKDTEVSTKAEGVRAPNQPAPVEGKLIKDHLFEESTFAPYIDELSDEQHRVSLVTAPVKITLTEIEPPLMTATQETIPEKQDLCLKPSPDTVPTVTVSEPEDDSPGSVTPPSSGTEPSAAESQGKGSVSEDELIAAIKEAKGLSYETTESPRPVGQVADKPKTKTRSGLPTIPSPLDQEASSAESGDSEIELVSEDPMASEDALPSGYVSFGHVSGPPPSPASPSIQYSILREEREAELDSELIIESCDASSASEESPKREQDSPPMKPGALDAIREETGSRATEERAPSHQGPVEPDPMLSFAPAAALQSRPEPSSGDGASVPEPPRSQQQKPEEEAVSSSQSPTATEIPGPLGSGLMPPLPFFNKQKAIDLLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAYLELEITLSQEQIQKYTDCLQLYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQVDQYLGLVRTHINTVVAKIQAKIPGAKRHAE

Target ORF information:

RefSeq Version	XM_006515324.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus reticulon 1 (Rtn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515324.2

ORF Insert Sequence:

ATGGCAGCTG TTCCCGATGC CCTCGACCAC TCTCCCTCTT CAACACTAAA AGATGGGGAA 
GGGGCATGTT ACACATCCCT GATTTCTGAC GTCTGCTATC CACCTCGGGA GGACTCTGCA 
TACTTTACTG GAATTCTTCA GAAGGAAAAT GGTCACATCA CCACTTCCGA GAGCCCTGAG 
GAGCCGGAGA CCCCCGGACC CTCGTTACCA GAAGTGCCCG GGATGGAGCC ACAGGGCTTA 
TTGAGTTCTG ATTCTGGAAT AGAGATGACT CCTGCTGAGT CCACTGAGGT GAACAAGATC 
CTAGCTGACC CCCTAGACCA GATGAAGGCA GAAGCCTACA AATACATTGA CATAACCAGG 
CCACAGGAGG CAAAAGGCCA GGAGGAGCAA CACCCTGGGC TAGAAGATAA AGACTTGGAC 
TTTAAAGACA AGGACACTGA AGTCTCCACA AAAGCAGAAG GGGTTCGAGC ACCAAACCAG 
CCAGCTCCTG TGGAGGGGAA ACTCATCAAG GACCACTTAT TCGAAGAATC TACCTTTGCT 
CCTTACATAG ATGAGCTCTC TGATGAGCAG CACAGGGTGT CTCTGGTCAC TGCCCCTGTC 
AAGATCACAC TGACGGAGAT CGAACCTCCT CTTATGACTG CCACACAAGA GACAATCCCC 
GAGAAGCAAG ACCTGTGTCT GAAGCCAAGT CCTGACACAG TGCCCACTGT CACTGTCTCA 
GAGCCTGAAG ATGACAGCCC AGGATCTGTC ACGCCTCCAT CTTCTGGAAC AGAACCATCT 
GCTGCAGAGT CCCAGGGGAA AGGCAGCGTC TCCGAGGATG AGTTGATTGC TGCCATTAAA 
GAAGCAAAGG GGTTATCATA TGAAACCACC GAGAGTCCAA GGCCAGTGGG CCAGGTGGCC 
GACAAACCCA AAACCAAGAC CAGGTCTGGG CTGCCCACCA TCCCCAGTCC CCTCGACCAG 
GAGGCCAGCA GTGCCGAGTC CGGAGACTCA GAAATTGAGC TGGTGTCAGA GGACCCCATG 
GCCTCTGAGG ATGCCCTGCC CTCTGGCTAT GTGAGCTTCG GTCATGTGTC GGGCCCGCCC 
CCCTCTCCTG CCTCTCCATC CATCCAGTAC AGCATCCTGC GGGAGGAGCG TGAGGCCGAG 
CTGGACAGCG AGCTCATCAT CGAGTCCTGT GATGCCTCCT CAGCCTCGGA GGAGAGCCCC 
AAGCGCGAGC AGGACTCGCC CCCGATGAAA CCGGGTGCCC TGGACGCCAT CCGCGAGGAG 
ACAGGCTCTC GGGCCACCGA AGAACGAGCG CCCAGCCACC AAGGCCCTGT GGAGCCCGAC 
CCGATGCTCA GCTTTGCCCC CGCTGCCGCC CTCCAATCCC GCCCAGAGCC ATCGTCTGGA 
GATGGAGCCT CGGTCCCGGA GCCGCCCAGA TCACAGCAGC AGAAGCCTGA AGAGGAAGCT 
GTGAGTTCCA GTCAAAGTCC CACGGCCACC GAAATTCCTG GACCACTTGG TTCTGGCCTC 
ATGCCCCCTC TGCCTTTCTT CAACAAGCAA AAAGCTATTG ACCTTCTGTA CTGGCGGGAC 
ATCAAGCAGA CTGGGATTGT GTTCGGGAGC TTCCTGCTGC TGCTCTTCTC CCTGACCCAG 
TTCAGCGTTG TGAGCGTCGT TGCCTACCTG GCCCTGGCCG CCCTCTCTGC CACCATCAGC 
TTCCGCATCT ACAAGTCCGT TCTACAAGCT GTGCAGAAAA CAGATGAGGG TCACCCTTTC 
AAGGCCTACC TGGAGCTGGA GATCACCCTG TCCCAGGAGC AGATCCAGAA GTACACAGAC 
TGCCTGCAGC TGTATGTGAA CAGCACTCTG AAGGAGCTAC GGAGGCTTTT CCTGGTCCAG 
GACCTGGTGG ATTCCTTAAA ATTTGCAGTC CTCATGTGGC TCCTGACCTA CGTTGGCGCG 
CTCTTCAATG GCCTGACCCT GCTGCTTATG GCTGTGGTTT CGATGTTTAC TCTACCTGTT 
GTGTACGTTA AGCACCAGGC ACAAGTTGAC CAATATCTGG GACTTGTGAG GACTCACATA 
AACACCGTCG TGGCAAAGAT CCAGGCTAAA ATCCCCGGCG CCAAGAGGCA CGCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reticulon-1 and Reduced Migration toward Chemoattractants by Macrophages Differentiated from the Bone Marrow of Ultraviolet-Irradiated and Ultraviolet-Chimeric Mice.
Journal of immunology (Baltimore, Md. : 1950)200(1)260-270(2018 Jan)
McGonigle TA,Dwyer AR,Greenland EL,Scott NM,Carter KW,Keane KN,Newsholme P,Goodridge HS,Pixley FJ,Hart PH

RTN1 and RTN3 protein are differentially associated with senile plaques in Alzheimer's brains.
Scientific reports7(1)6145(2017 Jul)
Shi Q,Ge Y,He W,Hu X,Yan R

Rtn1a-Mediated Endoplasmic Reticulum Stress in Podocyte Injury and Diabetic Nephropathy.
Scientific reports7(1)323(2017 Mar)
Fan Y,Zhang J,Xiao W,Lee K,Li Z,Wen J,He L,Gui D,Xue R,Jian G,Sheng X,He JC,Wang N

Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose Tissue.
Cell reports17(4)1008-1021(2016 10)
Duteil D,Tosic M,Lausecker F,Nenseth HZ,M?ller JM,Urban S,Willmann D,Petroll K,Messaddeq N,Arrigoni L,Manke T,Kornfeld JW,Br?ning JC,Zagoriy V,Meret M,Dengjel J,Kanouni T,Sch?le R

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

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Rtn1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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