Psme4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Psme4
Entrez Gene ID	103554
Full Name	proteasome (prosome, macropain) activator subunit 4
Synonyms	AA409398,AU041366,TEMO,mKIAA0077
General protein information	Preferred Names proteasome (prosome, macropain) activator subunit 4 Names proteasome activator complex subunit 4 proteasome activator PA200
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 A4

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314207.2	XP_017169696.1	proteasome activator complex subunit 4 isoform X1
XM_017314207.1	XP_017169696.1	proteasome activator complex subunit 4 isoform X1
XM_011243649.2	XP_011241951.1	proteasome activator complex subunit 4 isoform X2
NM_134013.3	NP_598774.2	proteasome activator complex subunit 4
XM_011243649.3	XP_011241951.1	proteasome activator complex subunit 4 isoform X2
XM_030245449.1	XP_030101309.1	proteasome activator complex subunit 4 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	PSME4	NP_055429.2
	LOC716580	XP_002808086.1
Rattus norvegicus (Norway rat)	Psme4	NP_001020311.2
Danio rerio (zebrafish)	LOC100535277	XP_005157069.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002597	XP_312339.5
Pan troglodytes (chimpanzee)	PSME4	XP_003309060.1
Bos taurus (cattle)	PSME4	NP_001192881.1
Caenorhabditis elegans (roundworm)	C14C10.5	NP_506153.1
Xenopus tropicalis (tropical clawed frog)	psme4	XP_002936960.1
Canis lupus familiaris (dog)	PSME4	XP_005626163.1
Mus musculus (house mouse)	Psme4	NP_598774.2
Gallus gallus (chicken)	PSME4	NP_001006393.2
Danio rerio (zebrafish)	psme4b	NP_001177949.1
Danio rerio (zebrafish)	psme4a	XP_005157072.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteasome activators, PA28? and PA200, play indispensable roles in male fertility.
Huang L, Haratake K, Miyahara H, Chiba T
Scientific reports623171(2016 Mar)

JMJD1C Exhibits Multiple Functions in Epigenetic Regulation during Spermatogenesis.
Nakajima R, Okano H, Noce T
PloS one11(9)e0163466(2016)

Acetylation-mediated proteasomal degradation of core histones during DNA repair and spermatogenesis.
Qian MX, Pang Y, Liu CH, Haratake K, Du BY, Ji DY, Wang GF, Zhu QQ, Song W, Yu Y, Zhang XX, Huang HT, Miao S, Chen LB, Zhang ZH, Liang YN, Liu S, Cha H, Yang D, Zhai Y, Komatsu T, Tsuruta F, Li H, Cao C, Li W, Li GH, Cheng Y, Chiba T, Wang L, Goldberg AL, Shen Y, Qiu XB
Cell153(5)1012-24(2013 May)

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Gineste C, De Winter JM, Kohl C, Witt CC, Giannesini B, Brohm K, Le Fur Y, Gretz N, Vilmen C, Pecchi E, Jubeau M, Cozzone PJ, Stienen GJ, Granzier H, Labeit S, Ottenheijm CA, Bendahan D, Gondin J
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)

Differential roles of proteasome and immunoproteasome regulators Pa28??, Pa28? and Pa200 in the degradation of oxidized proteins.
Pickering AM, Davies KJ
Archives of biochemistry and biophysics523(2)181-90(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrated that Psme3/Psme4 double KO (dKO) male mice are completely infertile and showed the up-regulation of several proteins involved in oxidative stress response in dKO sperms. These results suggest the important role of PA28gamma and PA200 in male fertility.
Title: Proteasome activators, PA28? and PA200, play indispensable roles in male fertility.

PA200/Blm10 specifically targets the core histones for acetylation-mediated degradation by proteasomes, providing mechanisms by which acetylation regulates histone degradation, DNA repair, and spermatogenesis.
Title: Acetylation-mediated proteasomal degradation of core histones during DNA repair and spermatogenesis.

The following Psme4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Psme4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu45012	XM_030245449.1 Latest version!	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu72061	XM_011243649.3 Latest version!	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu77695	XM_017314207.2 Latest version!	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu22366	NM_134013.3 Latest version!	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu77695	XM_017314207.1	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu72061	XM_011243649.2	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Psme4 ( XM_030245449.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101309.1 Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu45012
Clone ID Related Accession (Same CDS sequence)	XM_030245449.1
Accession Version	XM_030245449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	proteasome activator complex subunit 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTCCT TAGAGGAAAC AAAATTGTAT ACCGGACTGG AACATGACCT ATCCAGAGAA 
AACTACCGAG AAGTAATTGC TAGTGTCATA AGGAAACTTC TTAGCCACAT ACTTGATAAT 
TCAGAAGATG ATACCAAATC ACTGTTTCTC ATCATAAAGA TCATTGGAGA CCTTCTGCAC 
TTCCAAGGAT CTCATAAACA TGAATTTGAC TCTCGATGGA AAAGCTTTAA CTTAGTAAAG 
AAATCAATGG AAAATCGGCT CCATGGAAAA AAGCAACATA TCAGAGCATT ACTAATTGAC 
AGAGTGATGC TGCAGCATGA GTTGCGAACA CTAACTGTTG AGGGTTGTGA GTACAAAAAA 
ATACATCAAG ATATGATCAG AGATCTTCTT CGATTATCAA CAAGCTCATA CAGTCAGGTC 
AGAAACAAAG CTCAGCAAAC ATTTTTTGCT GCTTTGGGGG CATATAACTT CTGTTGCAGA 
GATATTATCC CCTTGGTTTT GGAATTCCTA CGGCCTGATA GAAAGGATGT CACACAGCAG 
CAGTTCAAGG GCGCCTTATA TTGTCTACTT GGAAATCACA GTGGTGTGTG CCTGGCAAAC 
CTTCATGATT GGGACTGTAT TGTGCAGACC TGGCCAGCAC TTGTTTCTTC AGGGCTTAGC 
CAAGCAATGT CACTGGAAAA GCCATCAATA GTGAGACTAT TTGATGACCT TGCTGAAAAG 
ATTCATAGAC AATATGAAAC AATTGGCTTG GATTTTACAA TTCCAAAGTC ATGTGCTGCA 
ATAGCAGAAT TACTTCAACA ATCAAAGAAT CCTTCCATTA GCCAGACACT ACTTAGCCCA 
GAAAAGATTA AAGAAGGACA GAAGCGTCAA CAAGATAAGA ATGCTGATGC CCTAAGGAAC 
TATGAATGTT TGGTAAATAC TTTACTAGAT GGTGTGGAGC AAAGAAATCT GCCTTGGAAA 
TTTGAGCATA TAGGAATCGG GCTTCTGTCT CTACTCCTAA GGGATGACAG AGTGTTGCCT 
CTTCGTGCCA TACGTTTTTT TGTTGAAAAT CTCAACCATG ATGCAATTGT TGTTCGAAAG 
ATGGCTATCT CAGCTGTTGC TGGTATTCTC AAGCAGCTAA AGAGAACTCA CAAAAAGCTG 
ACTATCAACC CTTATGAAAT CAGTGGATGT CCTAAACCTA CCAAAATTCT TGCTGGTGAT 
AGACCTGATA ACCATTGGTT GCATTATGAT AGTAAAAATA TACCAAGAAC AAAAAAAGAA 
TGGGAGTCAA GTTGCTTTGT TGAAAAAACT CACTGGGGAT ACTACAACTG GCCAAAGAAT 
ATGGTTGTTT ATGCTGGTGT GGAAGAACAG CCTAAACTTG GCAGAAGCAG GGAGGATATG 
ATAGAGGCAG AACAGATTAT ATATGATCGT TTTTCTGATC CTAAGTTTGT TGAGCAGTTA 
ATCACATTTC TATCTTTAGA AGACAGAAAA GGAAGAGATA AGTTTAGTCC CCGGCGTTTC 
TGCCTCTTTA AGGGTATATT TAGAAATTTT GATGATGCTT TTCTGCCTGT TCTGAAGCCC 
CATTTAGAAC GTTTGGTTGC AGATTCTCAT GAAAGTACCC AGAGATGTGT TGCAGAAATT 
ATAGCTGGTT TAATCAGAGG TTCTAAGCAT TGGACGTTTG AGAAGGTGGA GAAGCTTTGG 
GAGCTCCTGT GCCCGCTGCT GAGAACGGCA CTGTCCAACA TGACAGTGGA GACATACAAT 
GACTGGGGGA CCTGTATAGC CACTTCCTGT GAAAGCAGAG ATCCCCGGAA GCTTCACTGG 
CTTTTTGAGC TGCTCTTGGA GTCACCATTG AGTGGTGAAG GGGGGTCCTT TGTAGATGCA 
TGCCGACTCT ATGTGCTACA AGGTGGCCTT GCCCAGCAAG AGTGGAGAGT CCCTGAACTA 
TTGCACAGAC TTCTGAAGTA CTTGGAACCA AAACTCACCC AGGTTTACAA AAATGTCAGA 
GAAAGAATAG GAAGTGTGCT GACCTACATA TTTATGATAG ATGTTTCTTT GCCAAATACT 
GCTCCAACAA CATCTCCTTG TATCCCTGAG TTTACTGCTC GCGTCCTAGA AAAACTGAAA 
CCACTCACAG ATGTGGATGA AGAAATTCAG AACCATGTTA TGGAAGAAAA TGGCATTGGT 
GAAGAAGATG AACGAACTCA GGGCATTAAA CTCTTAAAAA CTATATTGAA ATGGCTTATG 
GCAAGTGCAG GAAGATCTTT TTCTACAGCA GTCAAAGAGC AACTTCAGCT TCTGCCTTTG 
TTCTTTAAGA TCGCTCCAGT GGAAAATGAC AATAGTTATG ATGAACTGAA AAGAGATGCA 
AAGCTCTGTT TGTCACTAAT GTCTCAGGGG TTGCTGTACC CTCAACAAGT GCCTTTGATA 
CTTCAGGTGC TAAGCCAGAC AGCAAGAAGC AGTTCTTGGC ATGCAAGATA TACAGTGCTG 
ACCTACCTCC AGACCATGGT ATTTTATAAC CTCTTTATTT TCCTAAACAA TGAAGATGCA 
GTTAAAGACA TCAGATGGCT GATTATATGC CTTTTGGAGG ATGAACAGTT AGAGGTTCGA 
GAAATGGCTG CTACCACCTT AAGTGGTCTG TTACAGTGTA ACTTCCTTAC CATGGACAGT 
GCTATGCAGA TTCATTTTGA GCAACTTTGC AAAACAAAAC TACCCAAGAA AAGAAAACGA 
GACCCTGGTT CTGTGGGAGA TACCATTCCT TCTGCAGAGT TGGTCAAACG TCATGCTGGT 
GTGCTTGGAC TTGGTGCATG TGTTCTTTCT AGCCCTTATG ATGTTCCTAC CTGGATGCCC 
CAGCTCCTTA TGAACCTCAG TGCACATTTG AATGATCCTC AGCCTATTGA GATGACTGTA 
AAGAAGACCT TATCCAATTT CCGAAGGACA CATCATGACA ACTGGCAAGA ACACAAGCAG 
CAGTTCACTG ATGACCAGCT GCTTGTCCTC ACTGACCTCC TTGTGTCACC ATGCTATTAT 
GCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101309.1
CDS	1614..4619
Translation	MVSLEETKLYTGLEHDLSRENYREVIASVIRKLLSHILDNSEDDTKSLFLIIKIIGDLLHFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRKDVTQQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPALVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCAAIAELLQQSKNPSISQTLLSPEKIKEGQKRQQDKNADALRNYECLVNTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPYEISGCPKPTKILAGDRPDNHWLHYDSKNIPRTKKEWESSCFVEKTHWGYYNWPKNMVVYAGVEEQPKLGRSREDMIEAEQIIYDRFSDPKFVEQLITFLSLEDRKGRDKFSPRRFCLFKGIFRNFDDAFLPVLKPHLERLVADSHESTQRCVAEIIAGLIRGSKHWTFEKVEKLWELLCPLLRTALSNMTVETYNDWGTCIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQGGLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTAPTTSPCIPEFTARVLEKLKPLTDVDEEIQNHVMEENGIGEEDERTQGIKLLKTILKWLMASAGRSFSTAVKEQLQLLPLFFKIAPVENDNSYDELKRDAKLCLSLMSQGLLYPQQVPLILQVLSQTARSSSWHARYTVLTYLQTMVFYNLFIFLNNEDAVKDIRWLIICLLEDEQLEVREMAATTLSGLLQCNFLTMDSAMQIHFEQLCKTKLPKKRKRDPGSVGDTIPSAELVKRHAGVLGLGACVLSSPYDVPTWMPQLLMNLSAHLNDPQPIEMTVKKTLSNFRRTHHDNWQEHKQQFTDDQLLVLTDLLVSPCYYA

Target ORF information:

RefSeq Version	XM_030245449.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245449.1

ORF Insert Sequence:

ATGGTCTCCT TAGAGGAAAC AAAATTGTAT ACCGGACTGG AACATGACCT ATCCAGAGAA 
AACTACCGAG AAGTAATTGC TAGTGTCATA AGGAAACTTC TTAGCCACAT ACTTGATAAT 
TCAGAAGATG ATACCAAATC ACTGTTTCTC ATCATAAAGA TCATTGGAGA CCTTCTGCAC 
TTCCAAGGAT CTCATAAACA TGAATTTGAC TCTCGATGGA AAAGCTTTAA CTTAGTAAAG 
AAATCAATGG AAAATCGGCT CCATGGAAAA AAGCAACATA TCAGAGCATT ACTAATTGAC 
AGAGTGATGC TGCAGCATGA GTTGCGAACA CTAACTGTTG AGGGTTGTGA GTACAAAAAA 
ATACATCAAG ATATGATCAG AGATCTTCTT CGATTATCAA CAAGCTCATA CAGTCAGGTC 
AGAAACAAAG CTCAGCAAAC ATTTTTTGCT GCTTTGGGGG CATATAACTT CTGTTGCAGA 
GATATTATCC CCTTGGTTTT GGAATTCCTA CGGCCTGATA GAAAGGATGT CACACAGCAG 
CAGTTCAAGG GCGCCTTATA TTGTCTACTT GGAAATCACA GTGGTGTGTG CCTGGCAAAC 
CTTCATGATT GGGACTGTAT TGTGCAGACC TGGCCAGCAC TTGTTTCTTC AGGGCTTAGC 
CAAGCAATGT CACTGGAAAA GCCATCAATA GTGAGACTAT TTGATGACCT TGCTGAAAAG 
ATTCATAGAC AATATGAAAC AATTGGCTTG GATTTTACAA TTCCAAAGTC ATGTGCTGCA 
ATAGCAGAAT TACTTCAACA ATCAAAGAAT CCTTCCATTA GCCAGACACT ACTTAGCCCA 
GAAAAGATTA AAGAAGGACA GAAGCGTCAA CAAGATAAGA ATGCTGATGC CCTAAGGAAC 
TATGAATGTT TGGTAAATAC TTTACTAGAT GGTGTGGAGC AAAGAAATCT GCCTTGGAAA 
TTTGAGCATA TAGGAATCGG GCTTCTGTCT CTACTCCTAA GGGATGACAG AGTGTTGCCT 
CTTCGTGCCA TACGTTTTTT TGTTGAAAAT CTCAACCATG ATGCAATTGT TGTTCGAAAG 
ATGGCTATCT CAGCTGTTGC TGGTATTCTC AAGCAGCTAA AGAGAACTCA CAAAAAGCTG 
ACTATCAACC CTTATGAAAT CAGTGGATGT CCTAAACCTA CCAAAATTCT TGCTGGTGAT 
AGACCTGATA ACCATTGGTT GCATTATGAT AGTAAAAATA TACCAAGAAC AAAAAAAGAA 
TGGGAGTCAA GTTGCTTTGT TGAAAAAACT CACTGGGGAT ACTACAACTG GCCAAAGAAT 
ATGGTTGTTT ATGCTGGTGT GGAAGAACAG CCTAAACTTG GCAGAAGCAG GGAGGATATG 
ATAGAGGCAG AACAGATTAT ATATGATCGT TTTTCTGATC CTAAGTTTGT TGAGCAGTTA 
ATCACATTTC TATCTTTAGA AGACAGAAAA GGAAGAGATA AGTTTAGTCC CCGGCGTTTC 
TGCCTCTTTA AGGGTATATT TAGAAATTTT GATGATGCTT TTCTGCCTGT TCTGAAGCCC 
CATTTAGAAC GTTTGGTTGC AGATTCTCAT GAAAGTACCC AGAGATGTGT TGCAGAAATT 
ATAGCTGGTT TAATCAGAGG TTCTAAGCAT TGGACGTTTG AGAAGGTGGA GAAGCTTTGG 
GAGCTCCTGT GCCCGCTGCT GAGAACGGCA CTGTCCAACA TGACAGTGGA GACATACAAT 
GACTGGGGGA CCTGTATAGC CACTTCCTGT GAAAGCAGAG ATCCCCGGAA GCTTCACTGG 
CTTTTTGAGC TGCTCTTGGA GTCACCATTG AGTGGTGAAG GGGGGTCCTT TGTAGATGCA 
TGCCGACTCT ATGTGCTACA AGGTGGCCTT GCCCAGCAAG AGTGGAGAGT CCCTGAACTA 
TTGCACAGAC TTCTGAAGTA CTTGGAACCA AAACTCACCC AGGTTTACAA AAATGTCAGA 
GAAAGAATAG GAAGTGTGCT GACCTACATA TTTATGATAG ATGTTTCTTT GCCAAATACT 
GCTCCAACAA CATCTCCTTG TATCCCTGAG TTTACTGCTC GCGTCCTAGA AAAACTGAAA 
CCACTCACAG ATGTGGATGA AGAAATTCAG AACCATGTTA TGGAAGAAAA TGGCATTGGT 
GAAGAAGATG AACGAACTCA GGGCATTAAA CTCTTAAAAA CTATATTGAA ATGGCTTATG 
GCAAGTGCAG GAAGATCTTT TTCTACAGCA GTCAAAGAGC AACTTCAGCT TCTGCCTTTG 
TTCTTTAAGA TCGCTCCAGT GGAAAATGAC AATAGTTATG ATGAACTGAA AAGAGATGCA 
AAGCTCTGTT TGTCACTAAT GTCTCAGGGG TTGCTGTACC CTCAACAAGT GCCTTTGATA 
CTTCAGGTGC TAAGCCAGAC AGCAAGAAGC AGTTCTTGGC ATGCAAGATA TACAGTGCTG 
ACCTACCTCC AGACCATGGT ATTTTATAAC CTCTTTATTT TCCTAAACAA TGAAGATGCA 
GTTAAAGACA TCAGATGGCT GATTATATGC CTTTTGGAGG ATGAACAGTT AGAGGTTCGA 
GAAATGGCTG CTACCACCTT AAGTGGTCTG TTACAGTGTA ACTTCCTTAC CATGGACAGT 
GCTATGCAGA TTCATTTTGA GCAACTTTGC AAAACAAAAC TACCCAAGAA AAGAAAACGA 
GACCCTGGTT CTGTGGGAGA TACCATTCCT TCTGCAGAGT TGGTCAAACG TCATGCTGGT 
GTGCTTGGAC TTGGTGCATG TGTTCTTTCT AGCCCTTATG ATGTTCCTAC CTGGATGCCC 
CAGCTCCTTA TGAACCTCAG TGCACATTTG AATGATCCTC AGCCTATTGA GATGACTGTA 
AAGAAGACCT TATCCAATTT CCGAAGGACA CATCATGACA ACTGGCAAGA ACACAAGCAG 
CAGTTCACTG ATGACCAGCT GCTTGTCCTC ACTGACCTCC TTGTGTCACC ATGCTATTAT 
GCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psme4 ( XM_011243649.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241951.1 Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu72061
Clone ID Related Accession (Same CDS sequence)	XM_011243649.2 , XM_011243649.3
Accession Version	XM_011243649.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	proteasome activator complex subunit 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011243649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTC CGGAGCGGGC GGGCGGCGGG GAGCCCCCCG AGCCTGGCGG GCGGCCGGTA 
CTGGGCCCGC GGGCCTTCGT CCCGCAGAAG GAGATCGTGT ACAACAAGCT TCTGCCCTAT 
GCCGAGCGGC TGGACGCCGA GTCCGACTTG CAGCTGGCAC AGATCAAGAG CAACCTGGGC 
CGGGCCGTGC AGCTCCAAGA GCTGTGGCCC GGGGGCCTCT TCTGGACGCG GAAACTCTCC 
ACTTATATCC GACTTTATGG GAGAAAATTT AGCAAAGAAG ATCATGTTCT TTTTATTAAG 
TTACTGTATG AGCTGGTATC AATTCCAAAA CTAGAAATCA GCATGATGCA GGGATTTGCC 
CGTCTCTTGA TCAACTTGTT AAAAAAAAAG GAACTTCTTT CAAGAGATGA TTTAGAGTTA 
CCTTGGAGAC CACTTTATGA CTTGGTAGAA AGAATATTAT ATTCCAAGAC AGAGCACCTG 
AGATTAAATT CATTTCCTAA TTCTATAGAA AATGTTCTCA AAACACTGGT GAAAAGCTGC 
CGACCATACT TTCCAGCAGA TTCAACAGCA GAAATGTTAG AAGAATGGCG GCCTTTAATG 
TGTCCTTTTG ATGTAACAAT GCAAAAGGCC ATCAGTTATT TTGAAATATT TCTTCCTACC 
TCCCTTCCTC CAGAACTGCA TCATAAAGGT TTTAAACTTT GGTTTGATGA ATTAATTGGC 
CTTTGGGTTT CAGTGCAAAA TCTCCCACAG TGGGAGGGGC AATTAGTAAA TCTCTTTGCT 
CGATTGGCTA CAGATAATAT AGGATACATA GATTGGGATC CATATGTACC GAAGATATTT 
ACAAGAATTC TGAGAAGCCT GAACCTCCCA GTGGGAAGCA GTCAAGTTTT AGTCCCAAGA 
TTTTTAACAA ATGCTTATGA TATAGGACAT GCTGTAATAT GGATCACCGC AATGATGGGT 
GGACCAAGTA AATTAGTACA AAAACATTTA GCTGGTTTGT TTAACAGCAT CACATCATTT 
TATCATCCTT CAAATAATGG GCGTTGGCTG AACAAGTTAA TGAAACTCCT TCAGCGGTTG 
CCAAACAGTG TTGTTAGGAG ATTACATCGT GAAAGATTCA AGAAGCCCTC CTGGTTAACT 
CCTGTGCCTG AAAGCCATAA GCTTACTGAT GAAGATGTTA CAGACTTTGT ACAATGCATT 
ATTCAGCCTG TCCTCTTGGC TATGTTTAGC AAAACTGGTA GTCTAGAAGC AGCCCAGGCT 
CTGCAGAACC TTGCACTTAT GAGACCTGAG TTAGTAATAC CCCCTGTGCT TGAAAGAACA 
TACCCTGCTT TAGAGACATT AACAGAACCT CACCAGCTCA CAGCTACTTT AAATTGTGTA 
ATTGGAGTAG CCCGAAGTTT GGTATCAGGC AGCAAATGGT TTCCCGAAGG GCCAACACAC 
ATGCTACCTC TGTTGATGAG AGCATTGCCT GGGGTAGATC CAAACGACTT TAGTAAGTGC 
ATGATCACAT TCCAATTTAT AGGAACATTT TCTACTCTGG TGCCTTTAGT AGACTGTTCA 
TCTGTACTGC AAGAAAGAAA TGACCTCACA GAAATTGAAA AAGAACTTTG TTCGGCAACA 
GCTGAATTTG AGGATTTTGT GTTACAATTT ATGGACAGAT GCTTTGGACT AATAGAAAGT 
AGTACATTGG AGCAAACAAG GGAAGAGACA GAAACAGAGA AAATGACTCA TTTGGAGAGT 
TTGGTTGAAT TAGGCTTATC TTCTACGTTT AGTACAATCC TCACACAATG TTCCAAAGAC 
ATATTTATGG TGGCCCTTCA GAAGGTCTTT AACTTTTCTG TTTCACATAT ATTTGAAACA 
AGAGTTGCAG GTCGCATGGT GGCAGACATG TGCCGAGCTG CTGTGAAGTG TTGCCCAGAA 
GAATCCCTGA AGCTGTTTGT TCCCCACTGC TATGGTGTTA TAACTCAGCT TACAATGAAT 
GATGATGTAT TGAATGAAGA AGAGCTAGAC AAGGAGTTGC TGTGGAACCT TCAGCTTCTG 
TCTGAGATTA CTCGTGTAGA CGGGAAGAAG TTACTTCTGT ATAGGGAGCA GCTTGTAAAG 
ATTCTCCAGC GAACCTTACA CCTGACCTGT AAGCAGGGCT ACACGCTGTC TTGCAACCTT 
CTGCATCATC TTCTTCGTTC TACCACACTA ATCTACCCTA CAGAGTACTG CAGTGTTCCG 
GGGGGCTTTA ACAAGCCGCC TTCTGAATAC TTCCCTGTCA AGGACTGGGG TAAGCCTGGA 
GATTTGTGGA ATCTGGGGAT CCAGTGGCAT GTTCCTTCTT CAGAAGAAGT CTCTTTTGCC 
TTCTATTTGT TAGACTCCTT TCTTCAGCCT GAGCTTATCA AACTCCAGTG TTGTGGAGAT 
GGAGAACTTG AAATGTCTAG AGATGATATT CTACAGAGTC TGACTATAGT ACACAGCTGC 
TTGATTGGTT CAGGGAACCT TCTACCTCCA TTGAAAGGAG AGGCAGTTAC TAACTTGGTA 
CCAAGTATGG TCTCCTTAGA GGAAACAAAA TTGTATACCG GACTGGAACA TGACCTATCC 
AGAGAAAACT ACCGAGAAGT AATTGCTAGT GTCATAAGGA AACTTCTTAG CCACATACTT 
GATAATTCAG AAGATGATAC CAAATCACTG TTTCTCATCA TAAAGATCAT TGGAGACCTT 
CTGCACTTCC AAGGATCTCA TAAACATGAA TTTGACTCTC GATGGAAAAG CTTTAACTTA 
GTAAAGAAAT CAATGGAAAA TCGGCTCCAT GGAAAAAAGC AACATATCAG AGCATTACTA 
ATTGACAGAG TGATGCTGCA GCATGAGTTG CGAACACTAA CTGTTGAGGG TTGTGAGTAC 
AAAAAAATAC ATCAAGATAT GATCAGAGAT CTTCTTCGAT TATCAACAAG CTCATACAGT 
CAGGTCAGAA ACAAAGCTCA GCAAACATTT TTTGCTGCTT TGGGGGCATA TAACTTCTGT 
TGCAGAGATA TTATCCCCTT GGTTTTGGAA TTCCTACGGC CTGATAGAAA GGATGTCACA 
CAGCAGCAGT TCAAGGGCGC CTTATATTGT CTACTTGGAA ATCACAGTGG TGTGTGCCTG 
GCAAACCTTC ATGATTGGGA CTGTATTGTG CAGACCTGGC CAGCACTTGT TTCTTCAGGG 
CTTAGCCAAG CAATGTCACT GGAAAAGCCA TCAATAGTGA GACTATTTGA TGACCTTGCT 
GAAAAGATTC ATAGACAATA TGAAACAATT GGCTTGGATT TTACAATTCC AAAGTCATGT 
GCTGCAATAG CAGAATTACT TCAACAATCA AAGAATCCTT CCATTAGCCA GACACTACTT 
AGCCCAGAAA AGATTAAAGA AGGACAGAAG CGTCAACAAG ATAAGAATGC TGATGCCCTA 
AGGAACTATG AATGTTTGGT AAATACTTTA CTAGATGGTG TGGAGCAAAG AAATCTGCCT 
TGGAAATTTG AGCATATAGG AATCGGGCTT CTGTCTCTAC TCCTAAGGGA TGACAGAGTG 
TTGCCTCTTC GTGCCATACG TTTTTTTGTT GAAAATCTCA ACCATGATGC AATTGTTGTT 
CGAAAGATGG CTATCTCAGC TGTTGCTGGT ATTCTCAAGC AGCTAAAGAG AACTCACAAA 
AAGCTGACTA TCAACCCTTA TGAAATCAGT GGATGTCCTA AACCTACCAA AATTCTTGCT 
GGTGATAGAC CTGATAACCA TTGGTTGCAT TATGATAGTA AAAATATACC AAGAACAAAA 
AAAGAATGGG AGTCAAGTTG CTTTGTTGAA AAAACTCACT GGGGATACTA CAACTGGCCA 
AAGAATATGG TTGTTTATGC TGGTGTGGAA GAACAGCCTA AACTTGGCAG AAGCAGGGAG 
GATATGATAG AGGCAGAACA GATTATATAT GATCGTTTTT CTGATCCTAA GTTTGTTGAG 
CAGTTAATCA CATTTCTATC TTTAGAAGAC AGAAAAGGAA GAGATAAGTT TAGTCCCCGG 
CGTTTCTGCC TCTTTAAGGG TATATTTAGA AATTTTGATG ATGCTTTTCT GCCTGTTCTG 
AAGCCCCATT TAGAACGTTT GGTTGCAGAT TCTCATGAAA GTACCCAGAG ATGTGTTGCA 
GAAATTATAG CTGGTTTAAT CAGAGGTTCT AAGCATTGGA CGTTTGAGAA GGTGGAGAAG 
CTTTGGGAGC TCCTGTGCCC GCTGCTGAGA ACGGCACTGT CCAACATGAC AGTGGAGACA 
TACAATGACT GGGGGACCTG TATAGCCACT TCCTGTGAAA GCAGAGATCC CCGGAAGCTT 
CACTGGCTTT TTGAGCTGCT CTTGGAGTCA CCATTGAGTG GTGAAGGGGG GTCCTTTGTA 
GATGCATGCC GACTCTATGT GCTACAAGGT GGCCTTGCCC AGCAAGAGTG GAGAGTCCCT 
GAACTATTGC ACAGACTTCT GAAGTACTTG GAACCAAAAC TCACCCAGGT TTACAAAAAT 
GTCAGAGAAA GAATAGGAAG TGTGCTGACC TACATATTTA TGATAGATGT TTCTTTGCCA 
AATACTGCTC CAACAACATC TCCTTGTATC CCTGAGTTTA CTGCTCGCGT CCTAGAAAAA 
CTGAAACCAC TCACAGATGT GGATGAAGAA ATTCAGAACC ATGTTATGGA AGAAAATGGC 
ATTGGTGAAG AAGATGAACG AACTCAGGGC ATTAAACTCT TAAAAACTAT ATTGAAATGG 
CTTATGGCAA GTGCAGGAAG ATCTTTTTCT ACAGCAGTCA AAGAGCAACT TCAGCTTCTG 
CCTTTGTTCT TTAAGGATCC TATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241951.1
CDS	206..5032
Translation	MEAPERAGGGEPPEPGGRPVLGPRAFVPQKEIVYNKLLPYAERLDAESDLQLAQIKSNLGRAVQLQELWPGGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFARLLINLLKKKELLSRDDLELPWRPLYDLVERILYSKTEHLRLNSFPNSIENVLKTLVKSCRPYFPADSTAEMLEEWRPLMCPFDVTMQKAISYFEIFLPTSLPPELHHKGFKLWFDELIGLWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIFTRILRSLNLPVGSSQVLVPRFLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWLNKLMKLLQRLPNSVVRRLHRERFKKPSWLTPVPESHKLTDEDVTDFVQCIIQPVLLAMFSKTGSLEAAQALQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLNCVIGVARSLVSGSKWFPEGPTHMLPLLMRALPGVDPNDFSKCMITFQFIGTFSTLVPLVDCSSVLQERNDLTEIEKELCSATAEFEDFVLQFMDRCFGLIESSTLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKDIFMVALQKVFNFSVSHIFETRVAGRMVADMCRAAVKCCPEESLKLFVPHCYGVITQLTMNDDVLNEEELDKELLWNLQLLSEITRVDGKKLLLYREQLVKILQRTLHLTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPSEYFPVKDWGKPGDLWNLGIQWHVPSSEEVSFAFYLLDSFLQPELIKLQCCGDGELEMSRDDILQSLTIVHSCLIGSGNLLPPLKGEAVTNLVPSMVSLEETKLYTGLEHDLSRENYREVIASVIRKLLSHILDNSEDDTKSLFLIIKIIGDLLHFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRKDVTQQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPALVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCAAIAELLQQSKNPSISQTLLSPEKIKEGQKRQQDKNADALRNYECLVNTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPYEISGCPKPTKILAGDRPDNHWLHYDSKNIPRTKKEWESSCFVEKTHWGYYNWPKNMVVYAGVEEQPKLGRSREDMIEAEQIIYDRFSDPKFVEQLITFLSLEDRKGRDKFSPRRFCLFKGIFRNFDDAFLPVLKPHLERLVADSHESTQRCVAEIIAGLIRGSKHWTFEKVEKLWELLCPLLRTALSNMTVETYNDWGTCIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQGGLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTAPTTSPCIPEFTARVLEKLKPLTDVDEEIQNHVMEENGIGEEDERTQGIKLLKTILKWLMASAGRSFSTAVKEQLQLLPLFFKDPM

Target ORF information:

RefSeq Version	XM_011243649.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243649.3

ORF Insert Sequence:

ATGGAGGCTC CGGAGCGGGC GGGCGGCGGG GAGCCCCCCG AGCCTGGCGG GCGGCCGGTA 
CTGGGCCCGC GGGCCTTCGT CCCGCAGAAG GAGATCGTGT ACAACAAGCT TCTGCCCTAT 
GCCGAGCGGC TGGACGCCGA GTCCGACTTG CAGCTGGCAC AGATCAAGAG CAACCTGGGC 
CGGGCCGTGC AGCTCCAAGA GCTGTGGCCC GGGGGCCTCT TCTGGACGCG GAAACTCTCC 
ACTTATATCC GACTTTATGG GAGAAAATTT AGCAAAGAAG ATCATGTTCT TTTTATTAAG 
TTACTGTATG AGCTGGTATC AATTCCAAAA CTAGAAATCA GCATGATGCA GGGATTTGCC 
CGTCTCTTGA TCAACTTGTT AAAAAAAAAG GAACTTCTTT CAAGAGATGA TTTAGAGTTA 
CCTTGGAGAC CACTTTATGA CTTGGTAGAA AGAATATTAT ATTCCAAGAC AGAGCACCTG 
AGATTAAATT CATTTCCTAA TTCTATAGAA AATGTTCTCA AAACACTGGT GAAAAGCTGC 
CGACCATACT TTCCAGCAGA TTCAACAGCA GAAATGTTAG AAGAATGGCG GCCTTTAATG 
TGTCCTTTTG ATGTAACAAT GCAAAAGGCC ATCAGTTATT TTGAAATATT TCTTCCTACC 
TCCCTTCCTC CAGAACTGCA TCATAAAGGT TTTAAACTTT GGTTTGATGA ATTAATTGGC 
CTTTGGGTTT CAGTGCAAAA TCTCCCACAG TGGGAGGGGC AATTAGTAAA TCTCTTTGCT 
CGATTGGCTA CAGATAATAT AGGATACATA GATTGGGATC CATATGTACC GAAGATATTT 
ACAAGAATTC TGAGAAGCCT GAACCTCCCA GTGGGAAGCA GTCAAGTTTT AGTCCCAAGA 
TTTTTAACAA ATGCTTATGA TATAGGACAT GCTGTAATAT GGATCACCGC AATGATGGGT 
GGACCAAGTA AATTAGTACA AAAACATTTA GCTGGTTTGT TTAACAGCAT CACATCATTT 
TATCATCCTT CAAATAATGG GCGTTGGCTG AACAAGTTAA TGAAACTCCT TCAGCGGTTG 
CCAAACAGTG TTGTTAGGAG ATTACATCGT GAAAGATTCA AGAAGCCCTC CTGGTTAACT 
CCTGTGCCTG AAAGCCATAA GCTTACTGAT GAAGATGTTA CAGACTTTGT ACAATGCATT 
ATTCAGCCTG TCCTCTTGGC TATGTTTAGC AAAACTGGTA GTCTAGAAGC AGCCCAGGCT 
CTGCAGAACC TTGCACTTAT GAGACCTGAG TTAGTAATAC CCCCTGTGCT TGAAAGAACA 
TACCCTGCTT TAGAGACATT AACAGAACCT CACCAGCTCA CAGCTACTTT AAATTGTGTA 
ATTGGAGTAG CCCGAAGTTT GGTATCAGGC AGCAAATGGT TTCCCGAAGG GCCAACACAC 
ATGCTACCTC TGTTGATGAG AGCATTGCCT GGGGTAGATC CAAACGACTT TAGTAAGTGC 
ATGATCACAT TCCAATTTAT AGGAACATTT TCTACTCTGG TGCCTTTAGT AGACTGTTCA 
TCTGTACTGC AAGAAAGAAA TGACCTCACA GAAATTGAAA AAGAACTTTG TTCGGCAACA 
GCTGAATTTG AGGATTTTGT GTTACAATTT ATGGACAGAT GCTTTGGACT AATAGAAAGT 
AGTACATTGG AGCAAACAAG GGAAGAGACA GAAACAGAGA AAATGACTCA TTTGGAGAGT 
TTGGTTGAAT TAGGCTTATC TTCTACGTTT AGTACAATCC TCACACAATG TTCCAAAGAC 
ATATTTATGG TGGCCCTTCA GAAGGTCTTT AACTTTTCTG TTTCACATAT ATTTGAAACA 
AGAGTTGCAG GTCGCATGGT GGCAGACATG TGCCGAGCTG CTGTGAAGTG TTGCCCAGAA 
GAATCCCTGA AGCTGTTTGT TCCCCACTGC TATGGTGTTA TAACTCAGCT TACAATGAAT 
GATGATGTAT TGAATGAAGA AGAGCTAGAC AAGGAGTTGC TGTGGAACCT TCAGCTTCTG 
TCTGAGATTA CTCGTGTAGA CGGGAAGAAG TTACTTCTGT ATAGGGAGCA GCTTGTAAAG 
ATTCTCCAGC GAACCTTACA CCTGACCTGT AAGCAGGGCT ACACGCTGTC TTGCAACCTT 
CTGCATCATC TTCTTCGTTC TACCACACTA ATCTACCCTA CAGAGTACTG CAGTGTTCCG 
GGGGGCTTTA ACAAGCCGCC TTCTGAATAC TTCCCTGTCA AGGACTGGGG TAAGCCTGGA 
GATTTGTGGA ATCTGGGGAT CCAGTGGCAT GTTCCTTCTT CAGAAGAAGT CTCTTTTGCC 
TTCTATTTGT TAGACTCCTT TCTTCAGCCT GAGCTTATCA AACTCCAGTG TTGTGGAGAT 
GGAGAACTTG AAATGTCTAG AGATGATATT CTACAGAGTC TGACTATAGT ACACAGCTGC 
TTGATTGGTT CAGGGAACCT TCTACCTCCA TTGAAAGGAG AGGCAGTTAC TAACTTGGTA 
CCAAGTATGG TCTCCTTAGA GGAAACAAAA TTGTATACCG GACTGGAACA TGACCTATCC 
AGAGAAAACT ACCGAGAAGT AATTGCTAGT GTCATAAGGA AACTTCTTAG CCACATACTT 
GATAATTCAG AAGATGATAC CAAATCACTG TTTCTCATCA TAAAGATCAT TGGAGACCTT 
CTGCACTTCC AAGGATCTCA TAAACATGAA TTTGACTCTC GATGGAAAAG CTTTAACTTA 
GTAAAGAAAT CAATGGAAAA TCGGCTCCAT GGAAAAAAGC AACATATCAG AGCATTACTA 
ATTGACAGAG TGATGCTGCA GCATGAGTTG CGAACACTAA CTGTTGAGGG TTGTGAGTAC 
AAAAAAATAC ATCAAGATAT GATCAGAGAT CTTCTTCGAT TATCAACAAG CTCATACAGT 
CAGGTCAGAA ACAAAGCTCA GCAAACATTT TTTGCTGCTT TGGGGGCATA TAACTTCTGT 
TGCAGAGATA TTATCCCCTT GGTTTTGGAA TTCCTACGGC CTGATAGAAA GGATGTCACA 
CAGCAGCAGT TCAAGGGCGC CTTATATTGT CTACTTGGAA ATCACAGTGG TGTGTGCCTG 
GCAAACCTTC ATGATTGGGA CTGTATTGTG CAGACCTGGC CAGCACTTGT TTCTTCAGGG 
CTTAGCCAAG CAATGTCACT GGAAAAGCCA TCAATAGTGA GACTATTTGA TGACCTTGCT 
GAAAAGATTC ATAGACAATA TGAAACAATT GGCTTGGATT TTACAATTCC AAAGTCATGT 
GCTGCAATAG CAGAATTACT TCAACAATCA AAGAATCCTT CCATTAGCCA GACACTACTT 
AGCCCAGAAA AGATTAAAGA AGGACAGAAG CGTCAACAAG ATAAGAATGC TGATGCCCTA 
AGGAACTATG AATGTTTGGT AAATACTTTA CTAGATGGTG TGGAGCAAAG AAATCTGCCT 
TGGAAATTTG AGCATATAGG AATCGGGCTT CTGTCTCTAC TCCTAAGGGA TGACAGAGTG 
TTGCCTCTTC GTGCCATACG TTTTTTTGTT GAAAATCTCA ACCATGATGC AATTGTTGTT 
CGAAAGATGG CTATCTCAGC TGTTGCTGGT ATTCTCAAGC AGCTAAAGAG AACTCACAAA 
AAGCTGACTA TCAACCCTTA TGAAATCAGT GGATGTCCTA AACCTACCAA AATTCTTGCT 
GGTGATAGAC CTGATAACCA TTGGTTGCAT TATGATAGTA AAAATATACC AAGAACAAAA 
AAAGAATGGG AGTCAAGTTG CTTTGTTGAA AAAACTCACT GGGGATACTA CAACTGGCCA 
AAGAATATGG TTGTTTATGC TGGTGTGGAA GAACAGCCTA AACTTGGCAG AAGCAGGGAG 
GATATGATAG AGGCAGAACA GATTATATAT GATCGTTTTT CTGATCCTAA GTTTGTTGAG 
CAGTTAATCA CATTTCTATC TTTAGAAGAC AGAAAAGGAA GAGATAAGTT TAGTCCCCGG 
CGTTTCTGCC TCTTTAAGGG TATATTTAGA AATTTTGATG ATGCTTTTCT GCCTGTTCTG 
AAGCCCCATT TAGAACGTTT GGTTGCAGAT TCTCATGAAA GTACCCAGAG ATGTGTTGCA 
GAAATTATAG CTGGTTTAAT CAGAGGTTCT AAGCATTGGA CGTTTGAGAA GGTGGAGAAG 
CTTTGGGAGC TCCTGTGCCC GCTGCTGAGA ACGGCACTGT CCAACATGAC AGTGGAGACA 
TACAATGACT GGGGGACCTG TATAGCCACT TCCTGTGAAA GCAGAGATCC CCGGAAGCTT 
CACTGGCTTT TTGAGCTGCT CTTGGAGTCA CCATTGAGTG GTGAAGGGGG GTCCTTTGTA 
GATGCATGCC GACTCTATGT GCTACAAGGT GGCCTTGCCC AGCAAGAGTG GAGAGTCCCT 
GAACTATTGC ACAGACTTCT GAAGTACTTG GAACCAAAAC TCACCCAGGT TTACAAAAAT 
GTCAGAGAAA GAATAGGAAG TGTGCTGACC TACATATTTA TGATAGATGT TTCTTTGCCA 
AATACTGCTC CAACAACATC TCCTTGTATC CCTGAGTTTA CTGCTCGCGT CCTAGAAAAA 
CTGAAACCAC TCACAGATGT GGATGAAGAA ATTCAGAACC ATGTTATGGA AGAAAATGGC 
ATTGGTGAAG AAGATGAACG AACTCAGGGC ATTAAACTCT TAAAAACTAT ATTGAAATGG 
CTTATGGCAA GTGCAGGAAG ATCTTTTTCT ACAGCAGTCA AAGAGCAACT TCAGCTTCTG 
CCTTTGTTCT TTAAGGATCC TATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psme4 ( XM_017314207.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169696.1 Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X1, mRNA.

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CloneID	OMu77695
Clone ID Related Accession (Same CDS sequence)	XM_017314207.2 , XM_017314207.1
Accession Version	XM_017314207.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	proteasome activator complex subunit 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTC CGGAGCGGGC GGGCGGCGGG GAGCCCCCCG AGCCTGGCGG GCGGCCGGTA 
CTGGGCCCGC GGGCCTTCGT CCCGCAGAAG GAGATCGTGT ACAACAAGCT TCTGCCCTAT 
GCCGAGCGGC TGGACGCCGA GTCCGACTTG CAGCTGGCAC AGATCAAGAG CAACCTGGGC 
CGGGCCGTGC AGCTCCAAGA GCTGTGGCCC GGGGGCCTCT TCTGGACGCG GAAACTCTCC 
ACTTATATCC GACTTTATGG GAGAAAATTT AGCAAAGAAG ATCATGTTCT TTTTATTAAG 
TTACTGTATG AGCTGGTATC AATTCCAAAA CTAGAAATCA GCATGATGCA GGGATTTGCC 
CGTCTCTTGA TCAACTTGTT AAAAAAAAAG GAACTTCTTT CAAGAGATGA TTTAGAGTTA 
CCTTGGAGAC CACTTTATGA CTTGGTAGAA AGAATATTAT ATTCCAAGAC AGAGCACCTG 
AGATTAAATT CATTTCCTAA TTCTATAGAA AATGTTCTCA AAACACTGGT GAAAAGCTGC 
CGACCATACT TTCCAGCAGA TTCAACAGCA GAAATGTTAG AAGAATGGCG GCCTTTAATG 
TGTCCTTTTG ATGTAACAAT GCAAAAGGCC ATCAGTTATT TTGAAATATT TCTTCCTACC 
TCCCTTCCTC CAGAACTGCA TCATAAAGGT TTTAAACTTT GGTTTGATGA ATTAATTGGC 
CTTTGGGTTT CAGTGCAAAA TCTCCCACAG TGGGAGGGGC AATTAGTAAA TCTCTTTGCT 
CGATTGGCTA CAGATAATAT AGGATACATA GATTGGGATC CATATGTACC GAAGATATTT 
ACAAGAATTC TGAGAAGCCT GAACCTCCCA GTGGGAAGCA GTCAAGTTTT AGTCCCAAGA 
TTTTTAACAA ATGCTTATGA TATAGGACAT GCTGTAATAT GGATCACCGC AATGATGGGT 
GGACCAAGTA AATTAGTACA AAAACATTTA GCTGGTTTGT TTAACAGCAT CACATCATTT 
TATCATCCTT CAAATAATGG GCGTTGGCTG AACAAGTTAA TGAAACTCCT TCAGCGGTTG 
CCAAACAGTG TTGTTAGGAG ATTACATCGT GAAAGATTCA AGAAGCCCTC CTGGTTAACT 
CCTGTGCCTG AAAGCCATAA GCTTACTGAT GAAGATGTTA CAGACTTTGT ACAATGCATT 
ATTCAGCCTG TCCTCTTGGC TATGTTTAGC AAAACTGGTA GTCTAGAAGC AGCCCAGGCT 
CTGCAGAACC TTGCACTTAT GAGACCTGAG TTAGTAATAC CCCCTGTGCT TGAAAGAACA 
TACCCTGCTT TAGAGACATT AACAGAACCT CACCAGCTCA CAGCTACTTT AAATTGTGTA 
ATTGGAGTAG CCCGAAGTTT GGTATCAGGC AGCAAATGGT TTCCCGAAGG GCCAACACAC 
ATGCTACCTC TGTTGATGAG AGCATTGCCT GGGGTAGATC CAAACGACTT TAGTAAGTGC 
ATGATCACAT TCCAATTTAT AGGAACATTT TCTACTCTGG TGCCTTTAGT AGACTGTTCA 
TCTGTACTGC AAGAAAGAAA TGACCTCACA GAAATTGAAA AAGAACTTTG TTCGGCAACA 
GCTGAATTTG AGGATTTTGT GTTACAATTT ATGGACAGAT GCTTTGGACT AATAGAAAGT 
AGTACATTGG AGCAAACAAG GGAAGAGACA GAAACAGAGA AAATGACTCA TTTGGAGAGT 
TTGGTTGAAT TAGGCTTATC TTCTACGTTT AGTACAATCC TCACACAATG TTCCAAAGAC 
ATATTTATGG TGGCCCTTCA GAAGGTCTTT AACTTTTCTG TTTCACATAT ATTTGAAACA 
AGAGTTGCAG GTCGCATGGT GGCAGACATG TGCCGAGCTG CTGTGAAGTG TTGCCCAGAA 
GAATCCCTGA AGCTGTTTGT TCCCCACTGC TATGGTGTTA TAACTCAGCT TACAATGAAT 
GATGATGTAT TGAATGAAGA AGAGCTAGAC AAGGAGTTGC TGTGGAACCT TCAGCTTCTG 
TCTGAGATTA CTCGTGTAGA CGGGAAGAAG TTACTTCTGT ATAGGGAGCA GCTTGTAAAG 
ATTCTCCAGC GAACCTTACA CCTGACCTGT AAGCAGGGCT ACACGCTGTC TTGCAACCTT 
CTGCATCATC TTCTTCGTTC TACCACACTA ATCTACCCTA CAGAGTACTG CAGTGTTCCG 
GGGGGCTTTA ACAAGCCGCC TTCTGAATAC TTCCCTGTCA AGGACTGGGG TAAGCCTGGA 
GATTTGTGGA ATCTGGGGAT CCAGTGGCAT GTTCCTTCTT CAGAAGAAGT CTCTTTTGCC 
TTCTATTTGT TAGACTCCTT TCTTCAGCCT GAGCTTATCA AACTCCAGTG TTGTGGAGAT 
GGAGAACTTG AAATGTCTAG AGATGATATT CTACAGAGTC TGACTATAGT ACACAGCTGC 
TTGATTGGTT CAGGGAACCT TCTACCTCCA TTGAAAGGAG AGGCAGTTAC TAACTTGGTA 
CCAAGTATGG TCTCCTTAGA GGAAACAAAA TTGTATACCG GACTGGAACA TGACCTATCC 
AGAGAAAACT ACCGAGAAGT AATTGCTAGT GTCATAAGGA AACTTCTTAG CCACATACTT 
GATAATTCAG AAGATGATAC CAAATCACTG TTTCTCATCA TAAAGATCAT TGGAGACCTT 
CTGCACTTCC AAGGATCTCA TAAACATGAA TTTGACTCTC GATGGAAAAG CTTTAACTTA 
GTAAAGAAAT CAATGGAAAA TCGGCTCCAT GGAAAAAAGC AACATATCAG AGCATTACTA 
ATTGACAGAG TGATGCTGCA GCATGAGTTG CGAACACTAA CTGTTGAGGG TTGTGAGTAC 
AAAAAAATAC ATCAAGATAT GATCAGAGAT CTTCTTCGAT TATCAACAAG CTCATACAGT 
CAGGTCAGAA ACAAAGCTCA GCAAACATTT TTTGCTGCTT TGGGGGCATA TAACTTCTGT 
TGCAGAGATA TTATCCCCTT GGTTTTGGAA TTCCTACGGC CTGATAGAAA GGATGTCACA 
CAGCAGCAGT TCAAGGGCGC CTTATATTGT CTACTTGGAA ATCACAGTGG TGTGTGCCTG 
GCAAACCTTC ATGATTGGGA CTGTATTGTG CAGACCTGGC CAGCACTTGT TTCTTCAGGG 
CTTAGCCAAG CAATGTCACT GGAAAAGCCA TCAATAGTGA GACTATTTGA TGACCTTGCT 
GAAAAGATTC ATAGACAATA TGAAACAATT GGCTTGGATT TTACAATTCC AAAGTCATGT 
GCTGCAATAG CAGAATTACT TCAACAATCA AAGAATCCTT CCATTAGCCA GACACTACTT 
AGCCCAGAAA AGATTAAAGA AGGACAGAAG CGTCAACAAG ATAAGAATGC TGATGCCCTA 
AGGAACTATG AATGTTTGGT AAATACTTTA CTAGATGGTG TGGAGCAAAG AAATCTGCCT 
TGGAAATTTG AGCATATAGG AATCGGGCTT CTGTCTCTAC TCCTAAGGGA TGACAGAGTG 
TTGCCTCTTC GTGCCATACG TTTTTTTGTT GAAAATCTCA ACCATGATGC AATTGTTGTT 
CGAAAGATGG CTATCTCAGC TGTTGCTGGT ATTCTCAAGC AGCTAAAGAG AACTCACAAA 
AAGCTGACTA TCAACCCTTA TGAAATCAGT GGATGTCCTA AACCTACCAA AATTCTTGCT 
GGTGATAGAC CTGATAACCA TTGGTTGCAT TATGATAGTA AAAATATACC AAGAACAAAA 
AAAGAATGGG AGTCAAGTTG CTTTGTTGAA AAAACTCACT GGGGATACTA CAACTGGCCA 
AAGAATATGG TTGTTTATGC TGGTGTGGAA GAACAGCCTA AACTTGGCAG AAGCAGGGAG 
GATATGATAG AGGCAGAACA GATTATATAT GATCGTTTTT CTGATCCTAA GTTTGTTGAG 
CAGTTAATCA CATTTCTATC TTTAGAAGAC AGAAAAGGAA GAGATAAGTT TAGTCCCCGG 
CGTTTCTGCC TCTTTAAGGG TATATTTAGA AATTTTGATG ATGCTTTTCT GCCTGTTCTG 
AAGCCCCATT TAGAACGTTT GGTTGCAGAT TCTCATGAAA GTACCCAGAG ATGTGTTGCA 
GAAATTATAG CTGGTTTAAT CAGAGGTTCT AAGCATTGGA CGTTTGAGAA GGTGGAGAAG 
CTTTGGGAGC TCCTGTGCCC GCTGCTGAGA ACGGCACTGT CCAACATGAC AGTGGAGACA 
TACAATGACT GGGGGACCTG TATAGCCACT TCCTGTGAAA GCAGAGATCC CCGGAAGCTT 
CACTGGCTTT TTGAGCTGCT CTTGGAGTCA CCATTGAGTG GTGAAGGGGG GTCCTTTGTA 
GATGCATGCC GACTCTATGT GCTACAAGGT GGCCTTGCCC AGCAAGAGTG GAGAGTCCCT 
GAACTATTGC ACAGACTTCT GAAGTACTTG GAACCAAAAC TCACCCAGGT TTACAAAAAT 
GTCAGAGAAA GAATAGGAAG TGTGCTGACC TACATATTTA TGATAGATGT TTCTTTGCCA 
AATACTGCTC CAACAACATC TCCTTGTATC CCTGAGTTTA CTGCTCGCGT CCTAGAAAAA 
CTGAAACCAC TCACAGATGT GGATGAAGAA ATTCAGAACC ATGTTATGGA AGAAAATGGC 
ATTGGTGAAG AAGATGAACG AACTCAGGGC ATTAAACTCT TAAAAACTAT ATTGAAATGG 
CTTATGGCAA GTGCAGGAAG ATCTTTTTCT ACAGCAGTCA AAGAGCAACT TCAGCTTCTG 
CCTTTGTTCT TTAAGCTGTC TTCAGACATA CCAGAAGAAG GCATCGGATT CTATTATAGA 
TCACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169696.1
CDS	206..5074
Translation	MEAPERAGGGEPPEPGGRPVLGPRAFVPQKEIVYNKLLPYAERLDAESDLQLAQIKSNLGRAVQLQELWPGGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFARLLINLLKKKELLSRDDLELPWRPLYDLVERILYSKTEHLRLNSFPNSIENVLKTLVKSCRPYFPADSTAEMLEEWRPLMCPFDVTMQKAISYFEIFLPTSLPPELHHKGFKLWFDELIGLWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIFTRILRSLNLPVGSSQVLVPRFLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWLNKLMKLLQRLPNSVVRRLHRERFKKPSWLTPVPESHKLTDEDVTDFVQCIIQPVLLAMFSKTGSLEAAQALQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLNCVIGVARSLVSGSKWFPEGPTHMLPLLMRALPGVDPNDFSKCMITFQFIGTFSTLVPLVDCSSVLQERNDLTEIEKELCSATAEFEDFVLQFMDRCFGLIESSTLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKDIFMVALQKVFNFSVSHIFETRVAGRMVADMCRAAVKCCPEESLKLFVPHCYGVITQLTMNDDVLNEEELDKELLWNLQLLSEITRVDGKKLLLYREQLVKILQRTLHLTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPSEYFPVKDWGKPGDLWNLGIQWHVPSSEEVSFAFYLLDSFLQPELIKLQCCGDGELEMSRDDILQSLTIVHSCLIGSGNLLPPLKGEAVTNLVPSMVSLEETKLYTGLEHDLSRENYREVIASVIRKLLSHILDNSEDDTKSLFLIIKIIGDLLHFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRKDVTQQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPALVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCAAIAELLQQSKNPSISQTLLSPEKIKEGQKRQQDKNADALRNYECLVNTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPYEISGCPKPTKILAGDRPDNHWLHYDSKNIPRTKKEWESSCFVEKTHWGYYNWPKNMVVYAGVEEQPKLGRSREDMIEAEQIIYDRFSDPKFVEQLITFLSLEDRKGRDKFSPRRFCLFKGIFRNFDDAFLPVLKPHLERLVADSHESTQRCVAEIIAGLIRGSKHWTFEKVEKLWELLCPLLRTALSNMTVETYNDWGTCIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQGGLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTAPTTSPCIPEFTARVLEKLKPLTDVDEEIQNHVMEENGIGEEDERTQGIKLLKTILKWLMASAGRSFSTAVKEQLQLLPLFFKLSSDIPEEGIGFYYRSL

Target ORF information:

RefSeq Version	XM_017314207.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314207.2

ORF Insert Sequence:

ATGGAGGCTC CGGAGCGGGC GGGCGGCGGG GAGCCCCCCG AGCCTGGCGG GCGGCCGGTA 
CTGGGCCCGC GGGCCTTCGT CCCGCAGAAG GAGATCGTGT ACAACAAGCT TCTGCCCTAT 
GCCGAGCGGC TGGACGCCGA GTCCGACTTG CAGCTGGCAC AGATCAAGAG CAACCTGGGC 
CGGGCCGTGC AGCTCCAAGA GCTGTGGCCC GGGGGCCTCT TCTGGACGCG GAAACTCTCC 
ACTTATATCC GACTTTATGG GAGAAAATTT AGCAAAGAAG ATCATGTTCT TTTTATTAAG 
TTACTGTATG AGCTGGTATC AATTCCAAAA CTAGAAATCA GCATGATGCA GGGATTTGCC 
CGTCTCTTGA TCAACTTGTT AAAAAAAAAG GAACTTCTTT CAAGAGATGA TTTAGAGTTA 
CCTTGGAGAC CACTTTATGA CTTGGTAGAA AGAATATTAT ATTCCAAGAC AGAGCACCTG 
AGATTAAATT CATTTCCTAA TTCTATAGAA AATGTTCTCA AAACACTGGT GAAAAGCTGC 
CGACCATACT TTCCAGCAGA TTCAACAGCA GAAATGTTAG AAGAATGGCG GCCTTTAATG 
TGTCCTTTTG ATGTAACAAT GCAAAAGGCC ATCAGTTATT TTGAAATATT TCTTCCTACC 
TCCCTTCCTC CAGAACTGCA TCATAAAGGT TTTAAACTTT GGTTTGATGA ATTAATTGGC 
CTTTGGGTTT CAGTGCAAAA TCTCCCACAG TGGGAGGGGC AATTAGTAAA TCTCTTTGCT 
CGATTGGCTA CAGATAATAT AGGATACATA GATTGGGATC CATATGTACC GAAGATATTT 
ACAAGAATTC TGAGAAGCCT GAACCTCCCA GTGGGAAGCA GTCAAGTTTT AGTCCCAAGA 
TTTTTAACAA ATGCTTATGA TATAGGACAT GCTGTAATAT GGATCACCGC AATGATGGGT 
GGACCAAGTA AATTAGTACA AAAACATTTA GCTGGTTTGT TTAACAGCAT CACATCATTT 
TATCATCCTT CAAATAATGG GCGTTGGCTG AACAAGTTAA TGAAACTCCT TCAGCGGTTG 
CCAAACAGTG TTGTTAGGAG ATTACATCGT GAAAGATTCA AGAAGCCCTC CTGGTTAACT 
CCTGTGCCTG AAAGCCATAA GCTTACTGAT GAAGATGTTA CAGACTTTGT ACAATGCATT 
ATTCAGCCTG TCCTCTTGGC TATGTTTAGC AAAACTGGTA GTCTAGAAGC AGCCCAGGCT 
CTGCAGAACC TTGCACTTAT GAGACCTGAG TTAGTAATAC CCCCTGTGCT TGAAAGAACA 
TACCCTGCTT TAGAGACATT AACAGAACCT CACCAGCTCA CAGCTACTTT AAATTGTGTA 
ATTGGAGTAG CCCGAAGTTT GGTATCAGGC AGCAAATGGT TTCCCGAAGG GCCAACACAC 
ATGCTACCTC TGTTGATGAG AGCATTGCCT GGGGTAGATC CAAACGACTT TAGTAAGTGC 
ATGATCACAT TCCAATTTAT AGGAACATTT TCTACTCTGG TGCCTTTAGT AGACTGTTCA 
TCTGTACTGC AAGAAAGAAA TGACCTCACA GAAATTGAAA AAGAACTTTG TTCGGCAACA 
GCTGAATTTG AGGATTTTGT GTTACAATTT ATGGACAGAT GCTTTGGACT AATAGAAAGT 
AGTACATTGG AGCAAACAAG GGAAGAGACA GAAACAGAGA AAATGACTCA TTTGGAGAGT 
TTGGTTGAAT TAGGCTTATC TTCTACGTTT AGTACAATCC TCACACAATG TTCCAAAGAC 
ATATTTATGG TGGCCCTTCA GAAGGTCTTT AACTTTTCTG TTTCACATAT ATTTGAAACA 
AGAGTTGCAG GTCGCATGGT GGCAGACATG TGCCGAGCTG CTGTGAAGTG TTGCCCAGAA 
GAATCCCTGA AGCTGTTTGT TCCCCACTGC TATGGTGTTA TAACTCAGCT TACAATGAAT 
GATGATGTAT TGAATGAAGA AGAGCTAGAC AAGGAGTTGC TGTGGAACCT TCAGCTTCTG 
TCTGAGATTA CTCGTGTAGA CGGGAAGAAG TTACTTCTGT ATAGGGAGCA GCTTGTAAAG 
ATTCTCCAGC GAACCTTACA CCTGACCTGT AAGCAGGGCT ACACGCTGTC TTGCAACCTT 
CTGCATCATC TTCTTCGTTC TACCACACTA ATCTACCCTA CAGAGTACTG CAGTGTTCCG 
GGGGGCTTTA ACAAGCCGCC TTCTGAATAC TTCCCTGTCA AGGACTGGGG TAAGCCTGGA 
GATTTGTGGA ATCTGGGGAT CCAGTGGCAT GTTCCTTCTT CAGAAGAAGT CTCTTTTGCC 
TTCTATTTGT TAGACTCCTT TCTTCAGCCT GAGCTTATCA AACTCCAGTG TTGTGGAGAT 
GGAGAACTTG AAATGTCTAG AGATGATATT CTACAGAGTC TGACTATAGT ACACAGCTGC 
TTGATTGGTT CAGGGAACCT TCTACCTCCA TTGAAAGGAG AGGCAGTTAC TAACTTGGTA 
CCAAGTATGG TCTCCTTAGA GGAAACAAAA TTGTATACCG GACTGGAACA TGACCTATCC 
AGAGAAAACT ACCGAGAAGT AATTGCTAGT GTCATAAGGA AACTTCTTAG CCACATACTT 
GATAATTCAG AAGATGATAC CAAATCACTG TTTCTCATCA TAAAGATCAT TGGAGACCTT 
CTGCACTTCC AAGGATCTCA TAAACATGAA TTTGACTCTC GATGGAAAAG CTTTAACTTA 
GTAAAGAAAT CAATGGAAAA TCGGCTCCAT GGAAAAAAGC AACATATCAG AGCATTACTA 
ATTGACAGAG TGATGCTGCA GCATGAGTTG CGAACACTAA CTGTTGAGGG TTGTGAGTAC 
AAAAAAATAC ATCAAGATAT GATCAGAGAT CTTCTTCGAT TATCAACAAG CTCATACAGT 
CAGGTCAGAA ACAAAGCTCA GCAAACATTT TTTGCTGCTT TGGGGGCATA TAACTTCTGT 
TGCAGAGATA TTATCCCCTT GGTTTTGGAA TTCCTACGGC CTGATAGAAA GGATGTCACA 
CAGCAGCAGT TCAAGGGCGC CTTATATTGT CTACTTGGAA ATCACAGTGG TGTGTGCCTG 
GCAAACCTTC ATGATTGGGA CTGTATTGTG CAGACCTGGC CAGCACTTGT TTCTTCAGGG 
CTTAGCCAAG CAATGTCACT GGAAAAGCCA TCAATAGTGA GACTATTTGA TGACCTTGCT 
GAAAAGATTC ATAGACAATA TGAAACAATT GGCTTGGATT TTACAATTCC AAAGTCATGT 
GCTGCAATAG CAGAATTACT TCAACAATCA AAGAATCCTT CCATTAGCCA GACACTACTT 
AGCCCAGAAA AGATTAAAGA AGGACAGAAG CGTCAACAAG ATAAGAATGC TGATGCCCTA 
AGGAACTATG AATGTTTGGT AAATACTTTA CTAGATGGTG TGGAGCAAAG AAATCTGCCT 
TGGAAATTTG AGCATATAGG AATCGGGCTT CTGTCTCTAC TCCTAAGGGA TGACAGAGTG 
TTGCCTCTTC GTGCCATACG TTTTTTTGTT GAAAATCTCA ACCATGATGC AATTGTTGTT 
CGAAAGATGG CTATCTCAGC TGTTGCTGGT ATTCTCAAGC AGCTAAAGAG AACTCACAAA 
AAGCTGACTA TCAACCCTTA TGAAATCAGT GGATGTCCTA AACCTACCAA AATTCTTGCT 
GGTGATAGAC CTGATAACCA TTGGTTGCAT TATGATAGTA AAAATATACC AAGAACAAAA 
AAAGAATGGG AGTCAAGTTG CTTTGTTGAA AAAACTCACT GGGGATACTA CAACTGGCCA 
AAGAATATGG TTGTTTATGC TGGTGTGGAA GAACAGCCTA AACTTGGCAG AAGCAGGGAG 
GATATGATAG AGGCAGAACA GATTATATAT GATCGTTTTT CTGATCCTAA GTTTGTTGAG 
CAGTTAATCA CATTTCTATC TTTAGAAGAC AGAAAAGGAA GAGATAAGTT TAGTCCCCGG 
CGTTTCTGCC TCTTTAAGGG TATATTTAGA AATTTTGATG ATGCTTTTCT GCCTGTTCTG 
AAGCCCCATT TAGAACGTTT GGTTGCAGAT TCTCATGAAA GTACCCAGAG ATGTGTTGCA 
GAAATTATAG CTGGTTTAAT CAGAGGTTCT AAGCATTGGA CGTTTGAGAA GGTGGAGAAG 
CTTTGGGAGC TCCTGTGCCC GCTGCTGAGA ACGGCACTGT CCAACATGAC AGTGGAGACA 
TACAATGACT GGGGGACCTG TATAGCCACT TCCTGTGAAA GCAGAGATCC CCGGAAGCTT 
CACTGGCTTT TTGAGCTGCT CTTGGAGTCA CCATTGAGTG GTGAAGGGGG GTCCTTTGTA 
GATGCATGCC GACTCTATGT GCTACAAGGT GGCCTTGCCC AGCAAGAGTG GAGAGTCCCT 
GAACTATTGC ACAGACTTCT GAAGTACTTG GAACCAAAAC TCACCCAGGT TTACAAAAAT 
GTCAGAGAAA GAATAGGAAG TGTGCTGACC TACATATTTA TGATAGATGT TTCTTTGCCA 
AATACTGCTC CAACAACATC TCCTTGTATC CCTGAGTTTA CTGCTCGCGT CCTAGAAAAA 
CTGAAACCAC TCACAGATGT GGATGAAGAA ATTCAGAACC ATGTTATGGA AGAAAATGGC 
ATTGGTGAAG AAGATGAACG AACTCAGGGC ATTAAACTCT TAAAAACTAT ATTGAAATGG 
CTTATGGCAA GTGCAGGAAG ATCTTTTTCT ACAGCAGTCA AAGAGCAACT TCAGCTTCTG 
CCTTTGTTCT TTAAGCTGTC TTCAGACATA CCAGAAGAAG GCATCGGATT CTATTATAGA 
TCACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psme4 ( NM_134013.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598774.2 Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), mRNA.

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CloneID	OMu22366
Clone ID Related Accession (Same CDS sequence)	NM_134013.3
Accession Version	NM_134013.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	proteasome activator complex subunit 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL662891.9, AF296169.1, CV557303.1, CV557020.1, CX566261.1, AK131932.1, AK169447.1 and BE944080.1. On Nov 15, 2006 this sequence version replaced NM_134013.2. ##Evidence-Data-START## Transcript exon combination :: AF296169.1, BC141381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCTC CGGAGCGGGC GGGCGGCGGG GAGCCCCCCG AGCCTGGCGG GCGGCCGGTA 
CTGGGCCCGC GGGCCTTCGT CCCGCAGAAG GAGATCGTGT ACAACAAGCT TCTGCCCTAT 
GCCGAGCGGC TGGACGCCGA GTCCGACTTG CAGCTGGCAC AGATCAAGAG CAACCTGGGC 
CGGGCCGTGC AGCTCCAAGA GCTGTGGCCC GGGGGCCTCT TCTGGACGCG GAAACTCTCC 
ACTTATATCC GACTTTATGG GAGAAAATTT AGCAAAGAAG ATCATGTTCT TTTTATTAAG 
TTACTGTATG AGCTGGTATC AATTCCAAAA CTAGAAATCA GCATGATGCA GGGATTTGCC 
CGTCTCTTGA TCAACTTGTT AAAAAAAAAG GAACTTCTTT CAAGAGATGA TTTAGAGTTA 
CCTTGGAGAC CACTTTATGA CTTGGTAGAA AGAATATTAT ATTCCAAGAC AGAGCACCTG 
AGATTAAATT CATTTCCTAA TTCTATAGAA AATGTTCTCA AAACACTGGT GAAAAGCTGC 
CGACCATACT TTCCAGCAGA TTCAACAGCA GAAATGTTAG AAGAATGGCG GCCTTTAATG 
TGTCCTTTTG ATGTAACAAT GCAAAAGGCC ATCAGTTATT TTGAAATATT TCTTCCTACC 
TCCCTTCCTC CAGAACTGCA TCATAAAGGT TTTAAACTTT GGTTTGATGA ATTAATTGGC 
CTTTGGGTTT CAGTGCAAAA TCTCCCACAG TGGGAGGGGC AATTAGTAAA TCTCTTTGCT 
CGATTGGCTA CAGATAATAT AGGATACATA GATTGGGATC CATATGTACC GAAGATATTT 
ACAAGAATTC TGAGAAGCCT GAACCTCCCA GTGGGAAGCA GTCAAGTTTT AGTCCCAAGA 
TTTTTAACAA ATGCTTATGA TATAGGACAT GCTGTAATAT GGATCACCGC AATGATGGGT 
GGACCAAGTA AATTAGTACA AAAACATTTA GCTGGTTTGT TTAACAGCAT CACATCATTT 
TATCATCCTT CAAATAATGG GCGTTGGCTG AACAAGTTAA TGAAACTCCT TCAGCGGTTG 
CCAAACAGTG TTGTTAGGAG ATTACATCGT GAAAGATTCA AGAAGCCCTC CTGGTTAACT 
CCTGTGCCTG AAAGCCATAA GCTTACTGAT GAAGATGTTA CAGACTTTGT ACAATGCATT 
ATTCAGCCTG TCCTCTTGGC TATGTTTAGC AAAACTGGTA GTCTAGAAGC AGCCCAGGCT 
CTGCAGAACC TTGCACTTAT GAGACCTGAG TTAGTAATAC CCCCTGTGCT TGAAAGAACA 
TACCCTGCTT TAGAGACATT AACAGAACCT CACCAGCTCA CAGCTACTTT AAATTGTGTA 
ATTGGAGTAG CCCGAAGTTT GGTATCAGGC AGCAAATGGT TTCCCGAAGG GCCAACACAC 
ATGCTACCTC TGTTGATGAG AGCATTGCCT GGGGTAGATC CAAACGACTT TAGTAAGTGC 
ATGATCACAT TCCAATTTAT AGGAACATTT TCTACTCTGG TGCCTTTAGT AGACTGTTCA 
TCTGTACTGC AAGAAAGAAA TGACCTCACA GAAATTGAAA AAGAACTTTG TTCGGCAACA 
GCTGAATTTG AGGATTTTGT GTTACAATTT ATGGACAGAT GCTTTGGACT AATAGAAAGT 
AGTACATTGG AGCAAACAAG GGAAGAGACA GAAACAGAGA AAATGACTCA TTTGGAGAGT 
TTGGTTGAAT TAGGCTTATC TTCTACGTTT AGTACAATCC TCACACAATG TTCCAAAGAC 
ATATTTATGG TGGCCCTTCA GAAGGTCTTT AACTTTTCTG TTTCACATAT ATTTGAAACA 
AGAGTTGCAG GTCGCATGGT GGCAGACATG TGCCGAGCTG CTGTGAAGTG TTGCCCAGAA 
GAATCCCTGA AGCTGTTTGT TCCCCACTGC TATGGTGTTA TAACTCAGCT TACAATGAAT 
GATGATGTAT TGAATGAAGA AGAGCTAGAC AAGGAGTTGC TGTGGAACCT TCAGCTTCTG 
TCTGAGATTA CTCGTGTAGA CGGGAAGAAG TTACTTCTGT ATAGGGAGCA GCTTGTAAAG 
ATTCTCCAGC GAACCTTACA CCTGACCTGT AAGCAGGGCT ACACGCTGTC TTGCAACCTT 
CTGCATCATC TTCTTCGTTC TACCACACTA ATCTACCCTA CAGAGTACTG CAGTGTTCCG 
GGGGGCTTTA ACAAGCCGCC TTCTGAATAC TTCCCTGTCA AGGACTGGGG TAAGCCTGGA 
GATTTGTGGA ATCTGGGGAT CCAGTGGCAT GTTCCTTCTT CAGAAGAAGT CTCTTTTGCC 
TTCTATTTGT TAGACTCCTT TCTTCAGCCT GAGCTTATCA AACTCCAGTG TTGTGGAGAT 
GGAGAACTTG AAATGTCTAG AGATGATATT CTACAGAGTC TGACTATAGT ACACAGCTGC 
TTGATTGGTT CAGGGAACCT TCTACCTCCA TTGAAAGGAG AGGCAGTTAC TAACTTGGTA 
CCAAGTATGG TCTCCTTAGA GGAAACAAAA TTGTATACCG GACTGGAACA TGACCTATCC 
AGAGAAAACT ACCGAGAAGT AATTGCTAGT GTCATAAGGA AACTTCTTAG CCACATACTT 
GATAATTCAG AAGATGATAC CAAATCACTG TTTCTCATCA TAAAGATCAT TGGAGACCTT 
CTGCACTTCC AAGGATCTCA TAAACATGAA TTTGACTCTC GATGGAAAAG CTTTAACTTA 
GTAAAGAAAT CAATGGAAAA TCGGCTCCAT GGAAAAAAGC AACATATCAG AGCATTACTA 
ATTGACAGAG TGATGCTGCA GCATGAGTTG CGAACACTAA CTGTTGAGGG TTGTGAGTAC 
AAAAAAATAC ATCAAGATAT GATCAGAGAT CTTCTTCGAT TATCAACAAG CTCATACAGT 
CAGGTCAGAA ACAAAGCTCA GCAAACATTT TTTGCTGCTT TGGGGGCATA TAACTTCTGT 
TGCAGAGATA TTATCCCCTT GGTTTTGGAA TTCCTACGGC CTGATAGAAA GGATGTCACA 
CAGCAGCAGT TCAAGGGCGC CTTATATTGT CTACTTGGAA ATCACAGTGG TGTGTGCCTG 
GCAAACCTTC ATGATTGGGA CTGTATTGTG CAGACCTGGC CAGCACTTGT TTCTTCAGGG 
CTTAGCCAAG CAATGTCACT GGAAAAGCCA TCAATAGTGA GACTATTTGA TGACCTTGCT 
GAAAAGATTC ATAGACAATA TGAAACAATT GGCTTGGATT TTACAATTCC AAAGTCATGT 
GCTGCAATAG CAGAATTACT TCAACAATCA AAGAATCCTT CCATTAGCCA GACACTACTT 
AGCCCAGAAA AGATTAAAGA AGGACAGAAG CGTCAACAAG ATAAGAATGC TGATGCCCTA 
AGGAACTATG AATGTTTGGT AAATACTTTA CTAGATGGTG TGGAGCAAAG AAATCTGCCT 
TGGAAATTTG AGCATATAGG AATCGGGCTT CTGTCTCTAC TCCTAAGGGA TGACAGAGTG 
TTGCCTCTTC GTGCCATACG TTTTTTTGTT GAAAATCTCA ACCATGATGC AATTGTTGTT 
CGAAAGATGG CTATCTCAGC TGTTGCTGGT ATTCTCAAGC AGCTAAAGAG AACTCACAAA 
AAGCTGACTA TCAACCCTTA TGAAATCAGT GGATGTCCTA AACCTACCAA AATTCTTGCT 
GGTGATAGAC CTGATAACCA TTGGTTGCAT TATGATAGTA AAAATATACC AAGAACAAAA 
AAAGAATGGG AGTCAAGTTG CTTTGTTGAA AAAACTCACT GGGGATACTA CAACTGGCCA 
AAGAATATGG TTGTTTATGC TGGTGTGGAA GAACAGCCTA AACTTGGCAG AAGCAGGGAG 
GATATGATAG AGGCAGAACA GATTATATAT GATCGTTTTT CTGATCCTAA GTTTGTTGAG 
CAGTTAATCA CATTTCTATC TTTAGAAGAC AGAAAAGGAA GAGATAAGTT TAGTCCCCGG 
CGTTTCTGCC TCTTTAAGGG TATATTTAGA AATTTTGATG ATGCTTTTCT GCCTGTTCTG 
AAGCCCCATT TAGAACGTTT GGTTGCAGAT TCTCATGAAA GTACCCAGAG ATGTGTTGCA 
GAAATTATAG CTGGTTTAAT CAGAGGTTCT AAGCATTGGA CGTTTGAGAA GGTGGAGAAG 
CTTTGGGAGC TCCTGTGCCC GCTGCTGAGA ACGGCACTGT CCAACATGAC AGTGGAGACA 
TACAATGACT GGGGGACCTG TATAGCCACT TCCTGTGAAA GCAGAGATCC CCGGAAGCTT 
CACTGGCTTT TTGAGCTGCT CTTGGAGTCA CCATTGAGTG GTGAAGGGGG GTCCTTTGTA 
GATGCATGCC GACTCTATGT GCTACAAGGT GGCCTTGCCC AGCAAGAGTG GAGAGTCCCT 
GAACTATTGC ACAGACTTCT GAAGTACTTG GAACCAAAAC TCACCCAGGT TTACAAAAAT 
GTCAGAGAAA GAATAGGAAG TGTGCTGACC TACATATTTA TGATAGATGT TTCTTTGCCA 
AATACTGCTC CAACAACATC TCCTTGTATC CCTGAGTTTA CTGCTCGCGT CCTAGAAAAA 
CTGAAACCAC TCACAGATGT GGATGAAGAA ATTCAGAACC ATGTTATGGA AGAAAATGGC 
ATTGGTGAAG AAGATGAACG AACTCAGGGC ATTAAACTCT TAAAAACTAT ATTGAAATGG 
CTTATGGCAA GTGCAGGAAG ATCTTTTTCT ACAGCAGTCA AAGAGCAACT TCAGCTTCTG 
CCTTTGTTCT TTAAGATCGC TCCAGTGGAA AATGACAATA GTTATGATGA ACTGAAAAGA 
GATGCAAAGC TCTGTTTGTC ACTAATGTCT CAGGGGTTGC TGTACCCTCA ACAAGTGCCT 
TTGATACTTC AGGTGCTAAG CCAGACAGCA AGAAGCAGTT CTTGGCATGC AAGATATACA 
GTGCTGACCT ACCTCCAGAC CATGGTATTT TATAACCTCT TTATTTTCCT AAACAATGAA 
GATGCAGTTA AAGACATCAG ATGGCTGATT ATATGCCTTT TGGAGGATGA ACAGTTAGAG 
GTTCGAGAAA TGGCTGCTAC CACCTTAAGT GGTCTGTTAC AGTGTAACTT CCTTACCATG 
GACAGTGCTA TGCAGATTCA TTTTGAGCAA CTTTGCAAAA CAAAACTACC CAAGAAAAGA 
AAACGAGACC CTGGTTCTGT GGGAGATACC ATTCCTTCTG CAGAGTTGGT CAAACGTCAT 
GCTGGTGTGC TTGGACTTGG TGCATGTGTT CTTTCTAGCC CTTATGATGT TCCTACCTGG 
ATGCCCCAGC TCCTTATGAA CCTCAGTGCA CATTTGAATG ATCCTCAGCC TATTGAGATG 
ACTGTAAAGA AGACCTTATC CAATTTCCGA AGGACACATC ATGACAACTG GCAAGAACAC 
AAGCAGCAGT TCACTGATGA CCAGCTGCTT GTCCTCACTG ACCTCCTTGT GTCACCATGC 
TATTATGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598774.2
CDS	209..5740
Translation	MEAPERAGGGEPPEPGGRPVLGPRAFVPQKEIVYNKLLPYAERLDAESDLQLAQIKSNLGRAVQLQELWPGGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFARLLINLLKKKELLSRDDLELPWRPLYDLVERILYSKTEHLRLNSFPNSIENVLKTLVKSCRPYFPADSTAEMLEEWRPLMCPFDVTMQKAISYFEIFLPTSLPPELHHKGFKLWFDELIGLWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIFTRILRSLNLPVGSSQVLVPRFLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWLNKLMKLLQRLPNSVVRRLHRERFKKPSWLTPVPESHKLTDEDVTDFVQCIIQPVLLAMFSKTGSLEAAQALQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLNCVIGVARSLVSGSKWFPEGPTHMLPLLMRALPGVDPNDFSKCMITFQFIGTFSTLVPLVDCSSVLQERNDLTEIEKELCSATAEFEDFVLQFMDRCFGLIESSTLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKDIFMVALQKVFNFSVSHIFETRVAGRMVADMCRAAVKCCPEESLKLFVPHCYGVITQLTMNDDVLNEEELDKELLWNLQLLSEITRVDGKKLLLYREQLVKILQRTLHLTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPSEYFPVKDWGKPGDLWNLGIQWHVPSSEEVSFAFYLLDSFLQPELIKLQCCGDGELEMSRDDILQSLTIVHSCLIGSGNLLPPLKGEAVTNLVPSMVSLEETKLYTGLEHDLSRENYREVIASVIRKLLSHILDNSEDDTKSLFLIIKIIGDLLHFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRKDVTQQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPALVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCAAIAELLQQSKNPSISQTLLSPEKIKEGQKRQQDKNADALRNYECLVNTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPYEISGCPKPTKILAGDRPDNHWLHYDSKNIPRTKKEWESSCFVEKTHWGYYNWPKNMVVYAGVEEQPKLGRSREDMIEAEQIIYDRFSDPKFVEQLITFLSLEDRKGRDKFSPRRFCLFKGIFRNFDDAFLPVLKPHLERLVADSHESTQRCVAEIIAGLIRGSKHWTFEKVEKLWELLCPLLRTALSNMTVETYNDWGTCIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQGGLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTAPTTSPCIPEFTARVLEKLKPLTDVDEEIQNHVMEENGIGEEDERTQGIKLLKTILKWLMASAGRSFSTAVKEQLQLLPLFFKIAPVENDNSYDELKRDAKLCLSLMSQGLLYPQQVPLILQVLSQTARSSSWHARYTVLTYLQTMVFYNLFIFLNNEDAVKDIRWLIICLLEDEQLEVREMAATTLSGLLQCNFLTMDSAMQIHFEQLCKTKLPKKRKRDPGSVGDTIPSAELVKRHAGVLGLGACVLSSPYDVPTWMPQLLMNLSAHLNDPQPIEMTVKKTLSNFRRTHHDNWQEHKQQFTDDQLLVLTDLLVSPCYYA

Target ORF information:

RefSeq Version	NM_134013.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134013.3

ORF Insert Sequence:

ATGGAGGCTC CGGAGCGGGC GGGCGGCGGG GAGCCCCCCG AGCCTGGCGG GCGGCCGGTA 
CTGGGCCCGC GGGCCTTCGT CCCGCAGAAG GAGATCGTGT ACAACAAGCT TCTGCCCTAT 
GCCGAGCGGC TGGACGCCGA GTCCGACTTG CAGCTGGCAC AGATCAAGAG CAACCTGGGC 
CGGGCCGTGC AGCTCCAAGA GCTGTGGCCC GGGGGCCTCT TCTGGACGCG GAAACTCTCC 
ACTTATATCC GACTTTATGG GAGAAAATTT AGCAAAGAAG ATCATGTTCT TTTTATTAAG 
TTACTGTATG AGCTGGTATC AATTCCAAAA CTAGAAATCA GCATGATGCA GGGATTTGCC 
CGTCTCTTGA TCAACTTGTT AAAAAAAAAG GAACTTCTTT CAAGAGATGA TTTAGAGTTA 
CCTTGGAGAC CACTTTATGA CTTGGTAGAA AGAATATTAT ATTCCAAGAC AGAGCACCTG 
AGATTAAATT CATTTCCTAA TTCTATAGAA AATGTTCTCA AAACACTGGT GAAAAGCTGC 
CGACCATACT TTCCAGCAGA TTCAACAGCA GAAATGTTAG AAGAATGGCG GCCTTTAATG 
TGTCCTTTTG ATGTAACAAT GCAAAAGGCC ATCAGTTATT TTGAAATATT TCTTCCTACC 
TCCCTTCCTC CAGAACTGCA TCATAAAGGT TTTAAACTTT GGTTTGATGA ATTAATTGGC 
CTTTGGGTTT CAGTGCAAAA TCTCCCACAG TGGGAGGGGC AATTAGTAAA TCTCTTTGCT 
CGATTGGCTA CAGATAATAT AGGATACATA GATTGGGATC CATATGTACC GAAGATATTT 
ACAAGAATTC TGAGAAGCCT GAACCTCCCA GTGGGAAGCA GTCAAGTTTT AGTCCCAAGA 
TTTTTAACAA ATGCTTATGA TATAGGACAT GCTGTAATAT GGATCACCGC AATGATGGGT 
GGACCAAGTA AATTAGTACA AAAACATTTA GCTGGTTTGT TTAACAGCAT CACATCATTT 
TATCATCCTT CAAATAATGG GCGTTGGCTG AACAAGTTAA TGAAACTCCT TCAGCGGTTG 
CCAAACAGTG TTGTTAGGAG ATTACATCGT GAAAGATTCA AGAAGCCCTC CTGGTTAACT 
CCTGTGCCTG AAAGCCATAA GCTTACTGAT GAAGATGTTA CAGACTTTGT ACAATGCATT 
ATTCAGCCTG TCCTCTTGGC TATGTTTAGC AAAACTGGTA GTCTAGAAGC AGCCCAGGCT 
CTGCAGAACC TTGCACTTAT GAGACCTGAG TTAGTAATAC CCCCTGTGCT TGAAAGAACA 
TACCCTGCTT TAGAGACATT AACAGAACCT CACCAGCTCA CAGCTACTTT AAATTGTGTA 
ATTGGAGTAG CCCGAAGTTT GGTATCAGGC AGCAAATGGT TTCCCGAAGG GCCAACACAC 
ATGCTACCTC TGTTGATGAG AGCATTGCCT GGGGTAGATC CAAACGACTT TAGTAAGTGC 
ATGATCACAT TCCAATTTAT AGGAACATTT TCTACTCTGG TGCCTTTAGT AGACTGTTCA 
TCTGTACTGC AAGAAAGAAA TGACCTCACA GAAATTGAAA AAGAACTTTG TTCGGCAACA 
GCTGAATTTG AGGATTTTGT GTTACAATTT ATGGACAGAT GCTTTGGACT AATAGAAAGT 
AGTACATTGG AGCAAACAAG GGAAGAGACA GAAACAGAGA AAATGACTCA TTTGGAGAGT 
TTGGTTGAAT TAGGCTTATC TTCTACGTTT AGTACAATCC TCACACAATG TTCCAAAGAC 
ATATTTATGG TGGCCCTTCA GAAGGTCTTT AACTTTTCTG TTTCACATAT ATTTGAAACA 
AGAGTTGCAG GTCGCATGGT GGCAGACATG TGCCGAGCTG CTGTGAAGTG TTGCCCAGAA 
GAATCCCTGA AGCTGTTTGT TCCCCACTGC TATGGTGTTA TAACTCAGCT TACAATGAAT 
GATGATGTAT TGAATGAAGA AGAGCTAGAC AAGGAGTTGC TGTGGAACCT TCAGCTTCTG 
TCTGAGATTA CTCGTGTAGA CGGGAAGAAG TTACTTCTGT ATAGGGAGCA GCTTGTAAAG 
ATTCTCCAGC GAACCTTACA CCTGACCTGT AAGCAGGGCT ACACGCTGTC TTGCAACCTT 
CTGCATCATC TTCTTCGTTC TACCACACTA ATCTACCCTA CAGAGTACTG CAGTGTTCCG 
GGGGGCTTTA ACAAGCCGCC TTCTGAATAC TTCCCTGTCA AGGACTGGGG TAAGCCTGGA 
GATTTGTGGA ATCTGGGGAT CCAGTGGCAT GTTCCTTCTT CAGAAGAAGT CTCTTTTGCC 
TTCTATTTGT TAGACTCCTT TCTTCAGCCT GAGCTTATCA AACTCCAGTG TTGTGGAGAT 
GGAGAACTTG AAATGTCTAG AGATGATATT CTACAGAGTC TGACTATAGT ACACAGCTGC 
TTGATTGGTT CAGGGAACCT TCTACCTCCA TTGAAAGGAG AGGCAGTTAC TAACTTGGTA 
CCAAGTATGG TCTCCTTAGA GGAAACAAAA TTGTATACCG GACTGGAACA TGACCTATCC 
AGAGAAAACT ACCGAGAAGT AATTGCTAGT GTCATAAGGA AACTTCTTAG CCACATACTT 
GATAATTCAG AAGATGATAC CAAATCACTG TTTCTCATCA TAAAGATCAT TGGAGACCTT 
CTGCACTTCC AAGGATCTCA TAAACATGAA TTTGACTCTC GATGGAAAAG CTTTAACTTA 
GTAAAGAAAT CAATGGAAAA TCGGCTCCAT GGAAAAAAGC AACATATCAG AGCATTACTA 
ATTGACAGAG TGATGCTGCA GCATGAGTTG CGAACACTAA CTGTTGAGGG TTGTGAGTAC 
AAAAAAATAC ATCAAGATAT GATCAGAGAT CTTCTTCGAT TATCAACAAG CTCATACAGT 
CAGGTCAGAA ACAAAGCTCA GCAAACATTT TTTGCTGCTT TGGGGGCATA TAACTTCTGT 
TGCAGAGATA TTATCCCCTT GGTTTTGGAA TTCCTACGGC CTGATAGAAA GGATGTCACA 
CAGCAGCAGT TCAAGGGCGC CTTATATTGT CTACTTGGAA ATCACAGTGG TGTGTGCCTG 
GCAAACCTTC ATGATTGGGA CTGTATTGTG CAGACCTGGC CAGCACTTGT TTCTTCAGGG 
CTTAGCCAAG CAATGTCACT GGAAAAGCCA TCAATAGTGA GACTATTTGA TGACCTTGCT 
GAAAAGATTC ATAGACAATA TGAAACAATT GGCTTGGATT TTACAATTCC AAAGTCATGT 
GCTGCAATAG CAGAATTACT TCAACAATCA AAGAATCCTT CCATTAGCCA GACACTACTT 
AGCCCAGAAA AGATTAAAGA AGGACAGAAG CGTCAACAAG ATAAGAATGC TGATGCCCTA 
AGGAACTATG AATGTTTGGT AAATACTTTA CTAGATGGTG TGGAGCAAAG AAATCTGCCT 
TGGAAATTTG AGCATATAGG AATCGGGCTT CTGTCTCTAC TCCTAAGGGA TGACAGAGTG 
TTGCCTCTTC GTGCCATACG TTTTTTTGTT GAAAATCTCA ACCATGATGC AATTGTTGTT 
CGAAAGATGG CTATCTCAGC TGTTGCTGGT ATTCTCAAGC AGCTAAAGAG AACTCACAAA 
AAGCTGACTA TCAACCCTTA TGAAATCAGT GGATGTCCTA AACCTACCAA AATTCTTGCT 
GGTGATAGAC CTGATAACCA TTGGTTGCAT TATGATAGTA AAAATATACC AAGAACAAAA 
AAAGAATGGG AGTCAAGTTG CTTTGTTGAA AAAACTCACT GGGGATACTA CAACTGGCCA 
AAGAATATGG TTGTTTATGC TGGTGTGGAA GAACAGCCTA AACTTGGCAG AAGCAGGGAG 
GATATGATAG AGGCAGAACA GATTATATAT GATCGTTTTT CTGATCCTAA GTTTGTTGAG 
CAGTTAATCA CATTTCTATC TTTAGAAGAC AGAAAAGGAA GAGATAAGTT TAGTCCCCGG 
CGTTTCTGCC TCTTTAAGGG TATATTTAGA AATTTTGATG ATGCTTTTCT GCCTGTTCTG 
AAGCCCCATT TAGAACGTTT GGTTGCAGAT TCTCATGAAA GTACCCAGAG ATGTGTTGCA 
GAAATTATAG CTGGTTTAAT CAGAGGTTCT AAGCATTGGA CGTTTGAGAA GGTGGAGAAG 
CTTTGGGAGC TCCTGTGCCC GCTGCTGAGA ACGGCACTGT CCAACATGAC AGTGGAGACA 
TACAATGACT GGGGGACCTG TATAGCCACT TCCTGTGAAA GCAGAGATCC CCGGAAGCTT 
CACTGGCTTT TTGAGCTGCT CTTGGAGTCA CCATTGAGTG GTGAAGGGGG GTCCTTTGTA 
GATGCATGCC GACTCTATGT GCTACAAGGT GGCCTTGCCC AGCAAGAGTG GAGAGTCCCT 
GAACTATTGC ACAGACTTCT GAAGTACTTG GAACCAAAAC TCACCCAGGT TTACAAAAAT 
GTCAGAGAAA GAATAGGAAG TGTGCTGACC TACATATTTA TGATAGATGT TTCTTTGCCA 
AATACTGCTC CAACAACATC TCCTTGTATC CCTGAGTTTA CTGCTCGCGT CCTAGAAAAA 
CTGAAACCAC TCACAGATGT GGATGAAGAA ATTCAGAACC ATGTTATGGA AGAAAATGGC 
ATTGGTGAAG AAGATGAACG AACTCAGGGC ATTAAACTCT TAAAAACTAT ATTGAAATGG 
CTTATGGCAA GTGCAGGAAG ATCTTTTTCT ACAGCAGTCA AAGAGCAACT TCAGCTTCTG 
CCTTTGTTCT TTAAGATCGC TCCAGTGGAA AATGACAATA GTTATGATGA ACTGAAAAGA 
GATGCAAAGC TCTGTTTGTC ACTAATGTCT CAGGGGTTGC TGTACCCTCA ACAAGTGCCT 
TTGATACTTC AGGTGCTAAG CCAGACAGCA AGAAGCAGTT CTTGGCATGC AAGATATACA 
GTGCTGACCT ACCTCCAGAC CATGGTATTT TATAACCTCT TTATTTTCCT AAACAATGAA 
GATGCAGTTA AAGACATCAG ATGGCTGATT ATATGCCTTT TGGAGGATGA ACAGTTAGAG 
GTTCGAGAAA TGGCTGCTAC CACCTTAAGT GGTCTGTTAC AGTGTAACTT CCTTACCATG 
GACAGTGCTA TGCAGATTCA TTTTGAGCAA CTTTGCAAAA CAAAACTACC CAAGAAAAGA 
AAACGAGACC CTGGTTCTGT GGGAGATACC ATTCCTTCTG CAGAGTTGGT CAAACGTCAT 
GCTGGTGTGC TTGGACTTGG TGCATGTGTT CTTTCTAGCC CTTATGATGT TCCTACCTGG 
ATGCCCCAGC TCCTTATGAA CCTCAGTGCA CATTTGAATG ATCCTCAGCC TATTGAGATG 
ACTGTAAAGA AGACCTTATC CAATTTCCGA AGGACACATC ATGACAACTG GCAAGAACAC 
AAGCAGCAGT TCACTGATGA CCAGCTGCTT GTCCTCACTG ACCTCCTTGT GTCACCATGC 
TATTATGCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psme4 ( XM_011243649.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241951.1 Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X2, mRNA.

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CloneID	OMu72061
Clone ID Related Accession (Same CDS sequence)	XM_011243649.2 , XM_011243649.3
Accession Version	XM_011243649.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	proteasome activator complex subunit 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTC CGGAGCGGGC GGGCGGCGGG GAGCCCCCCG AGCCTGGCGG GCGGCCGGTA 
CTGGGCCCGC GGGCCTTCGT CCCGCAGAAG GAGATCGTGT ACAACAAGCT TCTGCCCTAT 
GCCGAGCGGC TGGACGCCGA GTCCGACTTG CAGCTGGCAC AGATCAAGAG CAACCTGGGC 
CGGGCCGTGC AGCTCCAAGA GCTGTGGCCC GGGGGCCTCT TCTGGACGCG GAAACTCTCC 
ACTTATATCC GACTTTATGG GAGAAAATTT AGCAAAGAAG ATCATGTTCT TTTTATTAAG 
TTACTGTATG AGCTGGTATC AATTCCAAAA CTAGAAATCA GCATGATGCA GGGATTTGCC 
CGTCTCTTGA TCAACTTGTT AAAAAAAAAG GAACTTCTTT CAAGAGATGA TTTAGAGTTA 
CCTTGGAGAC CACTTTATGA CTTGGTAGAA AGAATATTAT ATTCCAAGAC AGAGCACCTG 
AGATTAAATT CATTTCCTAA TTCTATAGAA AATGTTCTCA AAACACTGGT GAAAAGCTGC 
CGACCATACT TTCCAGCAGA TTCAACAGCA GAAATGTTAG AAGAATGGCG GCCTTTAATG 
TGTCCTTTTG ATGTAACAAT GCAAAAGGCC ATCAGTTATT TTGAAATATT TCTTCCTACC 
TCCCTTCCTC CAGAACTGCA TCATAAAGGT TTTAAACTTT GGTTTGATGA ATTAATTGGC 
CTTTGGGTTT CAGTGCAAAA TCTCCCACAG TGGGAGGGGC AATTAGTAAA TCTCTTTGCT 
CGATTGGCTA CAGATAATAT AGGATACATA GATTGGGATC CATATGTACC GAAGATATTT 
ACAAGAATTC TGAGAAGCCT GAACCTCCCA GTGGGAAGCA GTCAAGTTTT AGTCCCAAGA 
TTTTTAACAA ATGCTTATGA TATAGGACAT GCTGTAATAT GGATCACCGC AATGATGGGT 
GGACCAAGTA AATTAGTACA AAAACATTTA GCTGGTTTGT TTAACAGCAT CACATCATTT 
TATCATCCTT CAAATAATGG GCGTTGGCTG AACAAGTTAA TGAAACTCCT TCAGCGGTTG 
CCAAACAGTG TTGTTAGGAG ATTACATCGT GAAAGATTCA AGAAGCCCTC CTGGTTAACT 
CCTGTGCCTG AAAGCCATAA GCTTACTGAT GAAGATGTTA CAGACTTTGT ACAATGCATT 
ATTCAGCCTG TCCTCTTGGC TATGTTTAGC AAAACTGGTA GTCTAGAAGC AGCCCAGGCT 
CTGCAGAACC TTGCACTTAT GAGACCTGAG TTAGTAATAC CCCCTGTGCT TGAAAGAACA 
TACCCTGCTT TAGAGACATT AACAGAACCT CACCAGCTCA CAGCTACTTT AAATTGTGTA 
ATTGGAGTAG CCCGAAGTTT GGTATCAGGC AGCAAATGGT TTCCCGAAGG GCCAACACAC 
ATGCTACCTC TGTTGATGAG AGCATTGCCT GGGGTAGATC CAAACGACTT TAGTAAGTGC 
ATGATCACAT TCCAATTTAT AGGAACATTT TCTACTCTGG TGCCTTTAGT AGACTGTTCA 
TCTGTACTGC AAGAAAGAAA TGACCTCACA GAAATTGAAA AAGAACTTTG TTCGGCAACA 
GCTGAATTTG AGGATTTTGT GTTACAATTT ATGGACAGAT GCTTTGGACT AATAGAAAGT 
AGTACATTGG AGCAAACAAG GGAAGAGACA GAAACAGAGA AAATGACTCA TTTGGAGAGT 
TTGGTTGAAT TAGGCTTATC TTCTACGTTT AGTACAATCC TCACACAATG TTCCAAAGAC 
ATATTTATGG TGGCCCTTCA GAAGGTCTTT AACTTTTCTG TTTCACATAT ATTTGAAACA 
AGAGTTGCAG GTCGCATGGT GGCAGACATG TGCCGAGCTG CTGTGAAGTG TTGCCCAGAA 
GAATCCCTGA AGCTGTTTGT TCCCCACTGC TATGGTGTTA TAACTCAGCT TACAATGAAT 
GATGATGTAT TGAATGAAGA AGAGCTAGAC AAGGAGTTGC TGTGGAACCT TCAGCTTCTG 
TCTGAGATTA CTCGTGTAGA CGGGAAGAAG TTACTTCTGT ATAGGGAGCA GCTTGTAAAG 
ATTCTCCAGC GAACCTTACA CCTGACCTGT AAGCAGGGCT ACACGCTGTC TTGCAACCTT 
CTGCATCATC TTCTTCGTTC TACCACACTA ATCTACCCTA CAGAGTACTG CAGTGTTCCG 
GGGGGCTTTA ACAAGCCGCC TTCTGAATAC TTCCCTGTCA AGGACTGGGG TAAGCCTGGA 
GATTTGTGGA ATCTGGGGAT CCAGTGGCAT GTTCCTTCTT CAGAAGAAGT CTCTTTTGCC 
TTCTATTTGT TAGACTCCTT TCTTCAGCCT GAGCTTATCA AACTCCAGTG TTGTGGAGAT 
GGAGAACTTG AAATGTCTAG AGATGATATT CTACAGAGTC TGACTATAGT ACACAGCTGC 
TTGATTGGTT CAGGGAACCT TCTACCTCCA TTGAAAGGAG AGGCAGTTAC TAACTTGGTA 
CCAAGTATGG TCTCCTTAGA GGAAACAAAA TTGTATACCG GACTGGAACA TGACCTATCC 
AGAGAAAACT ACCGAGAAGT AATTGCTAGT GTCATAAGGA AACTTCTTAG CCACATACTT 
GATAATTCAG AAGATGATAC CAAATCACTG TTTCTCATCA TAAAGATCAT TGGAGACCTT 
CTGCACTTCC AAGGATCTCA TAAACATGAA TTTGACTCTC GATGGAAAAG CTTTAACTTA 
GTAAAGAAAT CAATGGAAAA TCGGCTCCAT GGAAAAAAGC AACATATCAG AGCATTACTA 
ATTGACAGAG TGATGCTGCA GCATGAGTTG CGAACACTAA CTGTTGAGGG TTGTGAGTAC 
AAAAAAATAC ATCAAGATAT GATCAGAGAT CTTCTTCGAT TATCAACAAG CTCATACAGT 
CAGGTCAGAA ACAAAGCTCA GCAAACATTT TTTGCTGCTT TGGGGGCATA TAACTTCTGT 
TGCAGAGATA TTATCCCCTT GGTTTTGGAA TTCCTACGGC CTGATAGAAA GGATGTCACA 
CAGCAGCAGT TCAAGGGCGC CTTATATTGT CTACTTGGAA ATCACAGTGG TGTGTGCCTG 
GCAAACCTTC ATGATTGGGA CTGTATTGTG CAGACCTGGC CAGCACTTGT TTCTTCAGGG 
CTTAGCCAAG CAATGTCACT GGAAAAGCCA TCAATAGTGA GACTATTTGA TGACCTTGCT 
GAAAAGATTC ATAGACAATA TGAAACAATT GGCTTGGATT TTACAATTCC AAAGTCATGT 
GCTGCAATAG CAGAATTACT TCAACAATCA AAGAATCCTT CCATTAGCCA GACACTACTT 
AGCCCAGAAA AGATTAAAGA AGGACAGAAG CGTCAACAAG ATAAGAATGC TGATGCCCTA 
AGGAACTATG AATGTTTGGT AAATACTTTA CTAGATGGTG TGGAGCAAAG AAATCTGCCT 
TGGAAATTTG AGCATATAGG AATCGGGCTT CTGTCTCTAC TCCTAAGGGA TGACAGAGTG 
TTGCCTCTTC GTGCCATACG TTTTTTTGTT GAAAATCTCA ACCATGATGC AATTGTTGTT 
CGAAAGATGG CTATCTCAGC TGTTGCTGGT ATTCTCAAGC AGCTAAAGAG AACTCACAAA 
AAGCTGACTA TCAACCCTTA TGAAATCAGT GGATGTCCTA AACCTACCAA AATTCTTGCT 
GGTGATAGAC CTGATAACCA TTGGTTGCAT TATGATAGTA AAAATATACC AAGAACAAAA 
AAAGAATGGG AGTCAAGTTG CTTTGTTGAA AAAACTCACT GGGGATACTA CAACTGGCCA 
AAGAATATGG TTGTTTATGC TGGTGTGGAA GAACAGCCTA AACTTGGCAG AAGCAGGGAG 
GATATGATAG AGGCAGAACA GATTATATAT GATCGTTTTT CTGATCCTAA GTTTGTTGAG 
CAGTTAATCA CATTTCTATC TTTAGAAGAC AGAAAAGGAA GAGATAAGTT TAGTCCCCGG 
CGTTTCTGCC TCTTTAAGGG TATATTTAGA AATTTTGATG ATGCTTTTCT GCCTGTTCTG 
AAGCCCCATT TAGAACGTTT GGTTGCAGAT TCTCATGAAA GTACCCAGAG ATGTGTTGCA 
GAAATTATAG CTGGTTTAAT CAGAGGTTCT AAGCATTGGA CGTTTGAGAA GGTGGAGAAG 
CTTTGGGAGC TCCTGTGCCC GCTGCTGAGA ACGGCACTGT CCAACATGAC AGTGGAGACA 
TACAATGACT GGGGGACCTG TATAGCCACT TCCTGTGAAA GCAGAGATCC CCGGAAGCTT 
CACTGGCTTT TTGAGCTGCT CTTGGAGTCA CCATTGAGTG GTGAAGGGGG GTCCTTTGTA 
GATGCATGCC GACTCTATGT GCTACAAGGT GGCCTTGCCC AGCAAGAGTG GAGAGTCCCT 
GAACTATTGC ACAGACTTCT GAAGTACTTG GAACCAAAAC TCACCCAGGT TTACAAAAAT 
GTCAGAGAAA GAATAGGAAG TGTGCTGACC TACATATTTA TGATAGATGT TTCTTTGCCA 
AATACTGCTC CAACAACATC TCCTTGTATC CCTGAGTTTA CTGCTCGCGT CCTAGAAAAA 
CTGAAACCAC TCACAGATGT GGATGAAGAA ATTCAGAACC ATGTTATGGA AGAAAATGGC 
ATTGGTGAAG AAGATGAACG AACTCAGGGC ATTAAACTCT TAAAAACTAT ATTGAAATGG 
CTTATGGCAA GTGCAGGAAG ATCTTTTTCT ACAGCAGTCA AAGAGCAACT TCAGCTTCTG 
CCTTTGTTCT TTAAGGATCC TATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241951.1
CDS	255..5081
Translation	MEAPERAGGGEPPEPGGRPVLGPRAFVPQKEIVYNKLLPYAERLDAESDLQLAQIKSNLGRAVQLQELWPGGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFARLLINLLKKKELLSRDDLELPWRPLYDLVERILYSKTEHLRLNSFPNSIENVLKTLVKSCRPYFPADSTAEMLEEWRPLMCPFDVTMQKAISYFEIFLPTSLPPELHHKGFKLWFDELIGLWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIFTRILRSLNLPVGSSQVLVPRFLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWLNKLMKLLQRLPNSVVRRLHRERFKKPSWLTPVPESHKLTDEDVTDFVQCIIQPVLLAMFSKTGSLEAAQALQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLNCVIGVARSLVSGSKWFPEGPTHMLPLLMRALPGVDPNDFSKCMITFQFIGTFSTLVPLVDCSSVLQERNDLTEIEKELCSATAEFEDFVLQFMDRCFGLIESSTLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKDIFMVALQKVFNFSVSHIFETRVAGRMVADMCRAAVKCCPEESLKLFVPHCYGVITQLTMNDDVLNEEELDKELLWNLQLLSEITRVDGKKLLLYREQLVKILQRTLHLTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPSEYFPVKDWGKPGDLWNLGIQWHVPSSEEVSFAFYLLDSFLQPELIKLQCCGDGELEMSRDDILQSLTIVHSCLIGSGNLLPPLKGEAVTNLVPSMVSLEETKLYTGLEHDLSRENYREVIASVIRKLLSHILDNSEDDTKSLFLIIKIIGDLLHFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRKDVTQQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPALVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCAAIAELLQQSKNPSISQTLLSPEKIKEGQKRQQDKNADALRNYECLVNTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPYEISGCPKPTKILAGDRPDNHWLHYDSKNIPRTKKEWESSCFVEKTHWGYYNWPKNMVVYAGVEEQPKLGRSREDMIEAEQIIYDRFSDPKFVEQLITFLSLEDRKGRDKFSPRRFCLFKGIFRNFDDAFLPVLKPHLERLVADSHESTQRCVAEIIAGLIRGSKHWTFEKVEKLWELLCPLLRTALSNMTVETYNDWGTCIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQGGLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTAPTTSPCIPEFTARVLEKLKPLTDVDEEIQNHVMEENGIGEEDERTQGIKLLKTILKWLMASAGRSFSTAVKEQLQLLPLFFKDPM

Target ORF information:

RefSeq Version	XM_011243649.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243649.2

ORF Insert Sequence:

ATGGAGGCTC CGGAGCGGGC GGGCGGCGGG GAGCCCCCCG AGCCTGGCGG GCGGCCGGTA 
CTGGGCCCGC GGGCCTTCGT CCCGCAGAAG GAGATCGTGT ACAACAAGCT TCTGCCCTAT 
GCCGAGCGGC TGGACGCCGA GTCCGACTTG CAGCTGGCAC AGATCAAGAG CAACCTGGGC 
CGGGCCGTGC AGCTCCAAGA GCTGTGGCCC GGGGGCCTCT TCTGGACGCG GAAACTCTCC 
ACTTATATCC GACTTTATGG GAGAAAATTT AGCAAAGAAG ATCATGTTCT TTTTATTAAG 
TTACTGTATG AGCTGGTATC AATTCCAAAA CTAGAAATCA GCATGATGCA GGGATTTGCC 
CGTCTCTTGA TCAACTTGTT AAAAAAAAAG GAACTTCTTT CAAGAGATGA TTTAGAGTTA 
CCTTGGAGAC CACTTTATGA CTTGGTAGAA AGAATATTAT ATTCCAAGAC AGAGCACCTG 
AGATTAAATT CATTTCCTAA TTCTATAGAA AATGTTCTCA AAACACTGGT GAAAAGCTGC 
CGACCATACT TTCCAGCAGA TTCAACAGCA GAAATGTTAG AAGAATGGCG GCCTTTAATG 
TGTCCTTTTG ATGTAACAAT GCAAAAGGCC ATCAGTTATT TTGAAATATT TCTTCCTACC 
TCCCTTCCTC CAGAACTGCA TCATAAAGGT TTTAAACTTT GGTTTGATGA ATTAATTGGC 
CTTTGGGTTT CAGTGCAAAA TCTCCCACAG TGGGAGGGGC AATTAGTAAA TCTCTTTGCT 
CGATTGGCTA CAGATAATAT AGGATACATA GATTGGGATC CATATGTACC GAAGATATTT 
ACAAGAATTC TGAGAAGCCT GAACCTCCCA GTGGGAAGCA GTCAAGTTTT AGTCCCAAGA 
TTTTTAACAA ATGCTTATGA TATAGGACAT GCTGTAATAT GGATCACCGC AATGATGGGT 
GGACCAAGTA AATTAGTACA AAAACATTTA GCTGGTTTGT TTAACAGCAT CACATCATTT 
TATCATCCTT CAAATAATGG GCGTTGGCTG AACAAGTTAA TGAAACTCCT TCAGCGGTTG 
CCAAACAGTG TTGTTAGGAG ATTACATCGT GAAAGATTCA AGAAGCCCTC CTGGTTAACT 
CCTGTGCCTG AAAGCCATAA GCTTACTGAT GAAGATGTTA CAGACTTTGT ACAATGCATT 
ATTCAGCCTG TCCTCTTGGC TATGTTTAGC AAAACTGGTA GTCTAGAAGC AGCCCAGGCT 
CTGCAGAACC TTGCACTTAT GAGACCTGAG TTAGTAATAC CCCCTGTGCT TGAAAGAACA 
TACCCTGCTT TAGAGACATT AACAGAACCT CACCAGCTCA CAGCTACTTT AAATTGTGTA 
ATTGGAGTAG CCCGAAGTTT GGTATCAGGC AGCAAATGGT TTCCCGAAGG GCCAACACAC 
ATGCTACCTC TGTTGATGAG AGCATTGCCT GGGGTAGATC CAAACGACTT TAGTAAGTGC 
ATGATCACAT TCCAATTTAT AGGAACATTT TCTACTCTGG TGCCTTTAGT AGACTGTTCA 
TCTGTACTGC AAGAAAGAAA TGACCTCACA GAAATTGAAA AAGAACTTTG TTCGGCAACA 
GCTGAATTTG AGGATTTTGT GTTACAATTT ATGGACAGAT GCTTTGGACT AATAGAAAGT 
AGTACATTGG AGCAAACAAG GGAAGAGACA GAAACAGAGA AAATGACTCA TTTGGAGAGT 
TTGGTTGAAT TAGGCTTATC TTCTACGTTT AGTACAATCC TCACACAATG TTCCAAAGAC 
ATATTTATGG TGGCCCTTCA GAAGGTCTTT AACTTTTCTG TTTCACATAT ATTTGAAACA 
AGAGTTGCAG GTCGCATGGT GGCAGACATG TGCCGAGCTG CTGTGAAGTG TTGCCCAGAA 
GAATCCCTGA AGCTGTTTGT TCCCCACTGC TATGGTGTTA TAACTCAGCT TACAATGAAT 
GATGATGTAT TGAATGAAGA AGAGCTAGAC AAGGAGTTGC TGTGGAACCT TCAGCTTCTG 
TCTGAGATTA CTCGTGTAGA CGGGAAGAAG TTACTTCTGT ATAGGGAGCA GCTTGTAAAG 
ATTCTCCAGC GAACCTTACA CCTGACCTGT AAGCAGGGCT ACACGCTGTC TTGCAACCTT 
CTGCATCATC TTCTTCGTTC TACCACACTA ATCTACCCTA CAGAGTACTG CAGTGTTCCG 
GGGGGCTTTA ACAAGCCGCC TTCTGAATAC TTCCCTGTCA AGGACTGGGG TAAGCCTGGA 
GATTTGTGGA ATCTGGGGAT CCAGTGGCAT GTTCCTTCTT CAGAAGAAGT CTCTTTTGCC 
TTCTATTTGT TAGACTCCTT TCTTCAGCCT GAGCTTATCA AACTCCAGTG TTGTGGAGAT 
GGAGAACTTG AAATGTCTAG AGATGATATT CTACAGAGTC TGACTATAGT ACACAGCTGC 
TTGATTGGTT CAGGGAACCT TCTACCTCCA TTGAAAGGAG AGGCAGTTAC TAACTTGGTA 
CCAAGTATGG TCTCCTTAGA GGAAACAAAA TTGTATACCG GACTGGAACA TGACCTATCC 
AGAGAAAACT ACCGAGAAGT AATTGCTAGT GTCATAAGGA AACTTCTTAG CCACATACTT 
GATAATTCAG AAGATGATAC CAAATCACTG TTTCTCATCA TAAAGATCAT TGGAGACCTT 
CTGCACTTCC AAGGATCTCA TAAACATGAA TTTGACTCTC GATGGAAAAG CTTTAACTTA 
GTAAAGAAAT CAATGGAAAA TCGGCTCCAT GGAAAAAAGC AACATATCAG AGCATTACTA 
ATTGACAGAG TGATGCTGCA GCATGAGTTG CGAACACTAA CTGTTGAGGG TTGTGAGTAC 
AAAAAAATAC ATCAAGATAT GATCAGAGAT CTTCTTCGAT TATCAACAAG CTCATACAGT 
CAGGTCAGAA ACAAAGCTCA GCAAACATTT TTTGCTGCTT TGGGGGCATA TAACTTCTGT 
TGCAGAGATA TTATCCCCTT GGTTTTGGAA TTCCTACGGC CTGATAGAAA GGATGTCACA 
CAGCAGCAGT TCAAGGGCGC CTTATATTGT CTACTTGGAA ATCACAGTGG TGTGTGCCTG 
GCAAACCTTC ATGATTGGGA CTGTATTGTG CAGACCTGGC CAGCACTTGT TTCTTCAGGG 
CTTAGCCAAG CAATGTCACT GGAAAAGCCA TCAATAGTGA GACTATTTGA TGACCTTGCT 
GAAAAGATTC ATAGACAATA TGAAACAATT GGCTTGGATT TTACAATTCC AAAGTCATGT 
GCTGCAATAG CAGAATTACT TCAACAATCA AAGAATCCTT CCATTAGCCA GACACTACTT 
AGCCCAGAAA AGATTAAAGA AGGACAGAAG CGTCAACAAG ATAAGAATGC TGATGCCCTA 
AGGAACTATG AATGTTTGGT AAATACTTTA CTAGATGGTG TGGAGCAAAG AAATCTGCCT 
TGGAAATTTG AGCATATAGG AATCGGGCTT CTGTCTCTAC TCCTAAGGGA TGACAGAGTG 
TTGCCTCTTC GTGCCATACG TTTTTTTGTT GAAAATCTCA ACCATGATGC AATTGTTGTT 
CGAAAGATGG CTATCTCAGC TGTTGCTGGT ATTCTCAAGC AGCTAAAGAG AACTCACAAA 
AAGCTGACTA TCAACCCTTA TGAAATCAGT GGATGTCCTA AACCTACCAA AATTCTTGCT 
GGTGATAGAC CTGATAACCA TTGGTTGCAT TATGATAGTA AAAATATACC AAGAACAAAA 
AAAGAATGGG AGTCAAGTTG CTTTGTTGAA AAAACTCACT GGGGATACTA CAACTGGCCA 
AAGAATATGG TTGTTTATGC TGGTGTGGAA GAACAGCCTA AACTTGGCAG AAGCAGGGAG 
GATATGATAG AGGCAGAACA GATTATATAT GATCGTTTTT CTGATCCTAA GTTTGTTGAG 
CAGTTAATCA CATTTCTATC TTTAGAAGAC AGAAAAGGAA GAGATAAGTT TAGTCCCCGG 
CGTTTCTGCC TCTTTAAGGG TATATTTAGA AATTTTGATG ATGCTTTTCT GCCTGTTCTG 
AAGCCCCATT TAGAACGTTT GGTTGCAGAT TCTCATGAAA GTACCCAGAG ATGTGTTGCA 
GAAATTATAG CTGGTTTAAT CAGAGGTTCT AAGCATTGGA CGTTTGAGAA GGTGGAGAAG 
CTTTGGGAGC TCCTGTGCCC GCTGCTGAGA ACGGCACTGT CCAACATGAC AGTGGAGACA 
TACAATGACT GGGGGACCTG TATAGCCACT TCCTGTGAAA GCAGAGATCC CCGGAAGCTT 
CACTGGCTTT TTGAGCTGCT CTTGGAGTCA CCATTGAGTG GTGAAGGGGG GTCCTTTGTA 
GATGCATGCC GACTCTATGT GCTACAAGGT GGCCTTGCCC AGCAAGAGTG GAGAGTCCCT 
GAACTATTGC ACAGACTTCT GAAGTACTTG GAACCAAAAC TCACCCAGGT TTACAAAAAT 
GTCAGAGAAA GAATAGGAAG TGTGCTGACC TACATATTTA TGATAGATGT TTCTTTGCCA 
AATACTGCTC CAACAACATC TCCTTGTATC CCTGAGTTTA CTGCTCGCGT CCTAGAAAAA 
CTGAAACCAC TCACAGATGT GGATGAAGAA ATTCAGAACC ATGTTATGGA AGAAAATGGC 
ATTGGTGAAG AAGATGAACG AACTCAGGGC ATTAAACTCT TAAAAACTAT ATTGAAATGG 
CTTATGGCAA GTGCAGGAAG ATCTTTTTCT ACAGCAGTCA AAGAGCAACT TCAGCTTCTG 
CCTTTGTTCT TTAAGGATCC TATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Psme4 ( XM_017314207.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169696.1 Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X1, mRNA.

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CloneID	OMu77695
Clone ID Related Accession (Same CDS sequence)	XM_017314207.2 , XM_017314207.1
Accession Version	XM_017314207.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	proteasome activator complex subunit 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTC CGGAGCGGGC GGGCGGCGGG GAGCCCCCCG AGCCTGGCGG GCGGCCGGTA 
CTGGGCCCGC GGGCCTTCGT CCCGCAGAAG GAGATCGTGT ACAACAAGCT TCTGCCCTAT 
GCCGAGCGGC TGGACGCCGA GTCCGACTTG CAGCTGGCAC AGATCAAGAG CAACCTGGGC 
CGGGCCGTGC AGCTCCAAGA GCTGTGGCCC GGGGGCCTCT TCTGGACGCG GAAACTCTCC 
ACTTATATCC GACTTTATGG GAGAAAATTT AGCAAAGAAG ATCATGTTCT TTTTATTAAG 
TTACTGTATG AGCTGGTATC AATTCCAAAA CTAGAAATCA GCATGATGCA GGGATTTGCC 
CGTCTCTTGA TCAACTTGTT AAAAAAAAAG GAACTTCTTT CAAGAGATGA TTTAGAGTTA 
CCTTGGAGAC CACTTTATGA CTTGGTAGAA AGAATATTAT ATTCCAAGAC AGAGCACCTG 
AGATTAAATT CATTTCCTAA TTCTATAGAA AATGTTCTCA AAACACTGGT GAAAAGCTGC 
CGACCATACT TTCCAGCAGA TTCAACAGCA GAAATGTTAG AAGAATGGCG GCCTTTAATG 
TGTCCTTTTG ATGTAACAAT GCAAAAGGCC ATCAGTTATT TTGAAATATT TCTTCCTACC 
TCCCTTCCTC CAGAACTGCA TCATAAAGGT TTTAAACTTT GGTTTGATGA ATTAATTGGC 
CTTTGGGTTT CAGTGCAAAA TCTCCCACAG TGGGAGGGGC AATTAGTAAA TCTCTTTGCT 
CGATTGGCTA CAGATAATAT AGGATACATA GATTGGGATC CATATGTACC GAAGATATTT 
ACAAGAATTC TGAGAAGCCT GAACCTCCCA GTGGGAAGCA GTCAAGTTTT AGTCCCAAGA 
TTTTTAACAA ATGCTTATGA TATAGGACAT GCTGTAATAT GGATCACCGC AATGATGGGT 
GGACCAAGTA AATTAGTACA AAAACATTTA GCTGGTTTGT TTAACAGCAT CACATCATTT 
TATCATCCTT CAAATAATGG GCGTTGGCTG AACAAGTTAA TGAAACTCCT TCAGCGGTTG 
CCAAACAGTG TTGTTAGGAG ATTACATCGT GAAAGATTCA AGAAGCCCTC CTGGTTAACT 
CCTGTGCCTG AAAGCCATAA GCTTACTGAT GAAGATGTTA CAGACTTTGT ACAATGCATT 
ATTCAGCCTG TCCTCTTGGC TATGTTTAGC AAAACTGGTA GTCTAGAAGC AGCCCAGGCT 
CTGCAGAACC TTGCACTTAT GAGACCTGAG TTAGTAATAC CCCCTGTGCT TGAAAGAACA 
TACCCTGCTT TAGAGACATT AACAGAACCT CACCAGCTCA CAGCTACTTT AAATTGTGTA 
ATTGGAGTAG CCCGAAGTTT GGTATCAGGC AGCAAATGGT TTCCCGAAGG GCCAACACAC 
ATGCTACCTC TGTTGATGAG AGCATTGCCT GGGGTAGATC CAAACGACTT TAGTAAGTGC 
ATGATCACAT TCCAATTTAT AGGAACATTT TCTACTCTGG TGCCTTTAGT AGACTGTTCA 
TCTGTACTGC AAGAAAGAAA TGACCTCACA GAAATTGAAA AAGAACTTTG TTCGGCAACA 
GCTGAATTTG AGGATTTTGT GTTACAATTT ATGGACAGAT GCTTTGGACT AATAGAAAGT 
AGTACATTGG AGCAAACAAG GGAAGAGACA GAAACAGAGA AAATGACTCA TTTGGAGAGT 
TTGGTTGAAT TAGGCTTATC TTCTACGTTT AGTACAATCC TCACACAATG TTCCAAAGAC 
ATATTTATGG TGGCCCTTCA GAAGGTCTTT AACTTTTCTG TTTCACATAT ATTTGAAACA 
AGAGTTGCAG GTCGCATGGT GGCAGACATG TGCCGAGCTG CTGTGAAGTG TTGCCCAGAA 
GAATCCCTGA AGCTGTTTGT TCCCCACTGC TATGGTGTTA TAACTCAGCT TACAATGAAT 
GATGATGTAT TGAATGAAGA AGAGCTAGAC AAGGAGTTGC TGTGGAACCT TCAGCTTCTG 
TCTGAGATTA CTCGTGTAGA CGGGAAGAAG TTACTTCTGT ATAGGGAGCA GCTTGTAAAG 
ATTCTCCAGC GAACCTTACA CCTGACCTGT AAGCAGGGCT ACACGCTGTC TTGCAACCTT 
CTGCATCATC TTCTTCGTTC TACCACACTA ATCTACCCTA CAGAGTACTG CAGTGTTCCG 
GGGGGCTTTA ACAAGCCGCC TTCTGAATAC TTCCCTGTCA AGGACTGGGG TAAGCCTGGA 
GATTTGTGGA ATCTGGGGAT CCAGTGGCAT GTTCCTTCTT CAGAAGAAGT CTCTTTTGCC 
TTCTATTTGT TAGACTCCTT TCTTCAGCCT GAGCTTATCA AACTCCAGTG TTGTGGAGAT 
GGAGAACTTG AAATGTCTAG AGATGATATT CTACAGAGTC TGACTATAGT ACACAGCTGC 
TTGATTGGTT CAGGGAACCT TCTACCTCCA TTGAAAGGAG AGGCAGTTAC TAACTTGGTA 
CCAAGTATGG TCTCCTTAGA GGAAACAAAA TTGTATACCG GACTGGAACA TGACCTATCC 
AGAGAAAACT ACCGAGAAGT AATTGCTAGT GTCATAAGGA AACTTCTTAG CCACATACTT 
GATAATTCAG AAGATGATAC CAAATCACTG TTTCTCATCA TAAAGATCAT TGGAGACCTT 
CTGCACTTCC AAGGATCTCA TAAACATGAA TTTGACTCTC GATGGAAAAG CTTTAACTTA 
GTAAAGAAAT CAATGGAAAA TCGGCTCCAT GGAAAAAAGC AACATATCAG AGCATTACTA 
ATTGACAGAG TGATGCTGCA GCATGAGTTG CGAACACTAA CTGTTGAGGG TTGTGAGTAC 
AAAAAAATAC ATCAAGATAT GATCAGAGAT CTTCTTCGAT TATCAACAAG CTCATACAGT 
CAGGTCAGAA ACAAAGCTCA GCAAACATTT TTTGCTGCTT TGGGGGCATA TAACTTCTGT 
TGCAGAGATA TTATCCCCTT GGTTTTGGAA TTCCTACGGC CTGATAGAAA GGATGTCACA 
CAGCAGCAGT TCAAGGGCGC CTTATATTGT CTACTTGGAA ATCACAGTGG TGTGTGCCTG 
GCAAACCTTC ATGATTGGGA CTGTATTGTG CAGACCTGGC CAGCACTTGT TTCTTCAGGG 
CTTAGCCAAG CAATGTCACT GGAAAAGCCA TCAATAGTGA GACTATTTGA TGACCTTGCT 
GAAAAGATTC ATAGACAATA TGAAACAATT GGCTTGGATT TTACAATTCC AAAGTCATGT 
GCTGCAATAG CAGAATTACT TCAACAATCA AAGAATCCTT CCATTAGCCA GACACTACTT 
AGCCCAGAAA AGATTAAAGA AGGACAGAAG CGTCAACAAG ATAAGAATGC TGATGCCCTA 
AGGAACTATG AATGTTTGGT AAATACTTTA CTAGATGGTG TGGAGCAAAG AAATCTGCCT 
TGGAAATTTG AGCATATAGG AATCGGGCTT CTGTCTCTAC TCCTAAGGGA TGACAGAGTG 
TTGCCTCTTC GTGCCATACG TTTTTTTGTT GAAAATCTCA ACCATGATGC AATTGTTGTT 
CGAAAGATGG CTATCTCAGC TGTTGCTGGT ATTCTCAAGC AGCTAAAGAG AACTCACAAA 
AAGCTGACTA TCAACCCTTA TGAAATCAGT GGATGTCCTA AACCTACCAA AATTCTTGCT 
GGTGATAGAC CTGATAACCA TTGGTTGCAT TATGATAGTA AAAATATACC AAGAACAAAA 
AAAGAATGGG AGTCAAGTTG CTTTGTTGAA AAAACTCACT GGGGATACTA CAACTGGCCA 
AAGAATATGG TTGTTTATGC TGGTGTGGAA GAACAGCCTA AACTTGGCAG AAGCAGGGAG 
GATATGATAG AGGCAGAACA GATTATATAT GATCGTTTTT CTGATCCTAA GTTTGTTGAG 
CAGTTAATCA CATTTCTATC TTTAGAAGAC AGAAAAGGAA GAGATAAGTT TAGTCCCCGG 
CGTTTCTGCC TCTTTAAGGG TATATTTAGA AATTTTGATG ATGCTTTTCT GCCTGTTCTG 
AAGCCCCATT TAGAACGTTT GGTTGCAGAT TCTCATGAAA GTACCCAGAG ATGTGTTGCA 
GAAATTATAG CTGGTTTAAT CAGAGGTTCT AAGCATTGGA CGTTTGAGAA GGTGGAGAAG 
CTTTGGGAGC TCCTGTGCCC GCTGCTGAGA ACGGCACTGT CCAACATGAC AGTGGAGACA 
TACAATGACT GGGGGACCTG TATAGCCACT TCCTGTGAAA GCAGAGATCC CCGGAAGCTT 
CACTGGCTTT TTGAGCTGCT CTTGGAGTCA CCATTGAGTG GTGAAGGGGG GTCCTTTGTA 
GATGCATGCC GACTCTATGT GCTACAAGGT GGCCTTGCCC AGCAAGAGTG GAGAGTCCCT 
GAACTATTGC ACAGACTTCT GAAGTACTTG GAACCAAAAC TCACCCAGGT TTACAAAAAT 
GTCAGAGAAA GAATAGGAAG TGTGCTGACC TACATATTTA TGATAGATGT TTCTTTGCCA 
AATACTGCTC CAACAACATC TCCTTGTATC CCTGAGTTTA CTGCTCGCGT CCTAGAAAAA 
CTGAAACCAC TCACAGATGT GGATGAAGAA ATTCAGAACC ATGTTATGGA AGAAAATGGC 
ATTGGTGAAG AAGATGAACG AACTCAGGGC ATTAAACTCT TAAAAACTAT ATTGAAATGG 
CTTATGGCAA GTGCAGGAAG ATCTTTTTCT ACAGCAGTCA AAGAGCAACT TCAGCTTCTG 
CCTTTGTTCT TTAAGCTGTC TTCAGACATA CCAGAAGAAG GCATCGGATT CTATTATAGA 
TCACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169696.1
CDS	256..5124
Translation	MEAPERAGGGEPPEPGGRPVLGPRAFVPQKEIVYNKLLPYAERLDAESDLQLAQIKSNLGRAVQLQELWPGGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFARLLINLLKKKELLSRDDLELPWRPLYDLVERILYSKTEHLRLNSFPNSIENVLKTLVKSCRPYFPADSTAEMLEEWRPLMCPFDVTMQKAISYFEIFLPTSLPPELHHKGFKLWFDELIGLWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIFTRILRSLNLPVGSSQVLVPRFLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWLNKLMKLLQRLPNSVVRRLHRERFKKPSWLTPVPESHKLTDEDVTDFVQCIIQPVLLAMFSKTGSLEAAQALQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLNCVIGVARSLVSGSKWFPEGPTHMLPLLMRALPGVDPNDFSKCMITFQFIGTFSTLVPLVDCSSVLQERNDLTEIEKELCSATAEFEDFVLQFMDRCFGLIESSTLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKDIFMVALQKVFNFSVSHIFETRVAGRMVADMCRAAVKCCPEESLKLFVPHCYGVITQLTMNDDVLNEEELDKELLWNLQLLSEITRVDGKKLLLYREQLVKILQRTLHLTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPSEYFPVKDWGKPGDLWNLGIQWHVPSSEEVSFAFYLLDSFLQPELIKLQCCGDGELEMSRDDILQSLTIVHSCLIGSGNLLPPLKGEAVTNLVPSMVSLEETKLYTGLEHDLSRENYREVIASVIRKLLSHILDNSEDDTKSLFLIIKIIGDLLHFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRKDVTQQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPALVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCAAIAELLQQSKNPSISQTLLSPEKIKEGQKRQQDKNADALRNYECLVNTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPYEISGCPKPTKILAGDRPDNHWLHYDSKNIPRTKKEWESSCFVEKTHWGYYNWPKNMVVYAGVEEQPKLGRSREDMIEAEQIIYDRFSDPKFVEQLITFLSLEDRKGRDKFSPRRFCLFKGIFRNFDDAFLPVLKPHLERLVADSHESTQRCVAEIIAGLIRGSKHWTFEKVEKLWELLCPLLRTALSNMTVETYNDWGTCIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQGGLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTAPTTSPCIPEFTARVLEKLKPLTDVDEEIQNHVMEENGIGEEDERTQGIKLLKTILKWLMASAGRSFSTAVKEQLQLLPLFFKLSSDIPEEGIGFYYRSL

Target ORF information:

RefSeq Version	XM_017314207.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus proteasome (prosome, macropain) activator subunit 4 (Psme4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314207.1

ORF Insert Sequence:

ATGGAGGCTC CGGAGCGGGC GGGCGGCGGG GAGCCCCCCG AGCCTGGCGG GCGGCCGGTA 
CTGGGCCCGC GGGCCTTCGT CCCGCAGAAG GAGATCGTGT ACAACAAGCT TCTGCCCTAT 
GCCGAGCGGC TGGACGCCGA GTCCGACTTG CAGCTGGCAC AGATCAAGAG CAACCTGGGC 
CGGGCCGTGC AGCTCCAAGA GCTGTGGCCC GGGGGCCTCT TCTGGACGCG GAAACTCTCC 
ACTTATATCC GACTTTATGG GAGAAAATTT AGCAAAGAAG ATCATGTTCT TTTTATTAAG 
TTACTGTATG AGCTGGTATC AATTCCAAAA CTAGAAATCA GCATGATGCA GGGATTTGCC 
CGTCTCTTGA TCAACTTGTT AAAAAAAAAG GAACTTCTTT CAAGAGATGA TTTAGAGTTA 
CCTTGGAGAC CACTTTATGA CTTGGTAGAA AGAATATTAT ATTCCAAGAC AGAGCACCTG 
AGATTAAATT CATTTCCTAA TTCTATAGAA AATGTTCTCA AAACACTGGT GAAAAGCTGC 
CGACCATACT TTCCAGCAGA TTCAACAGCA GAAATGTTAG AAGAATGGCG GCCTTTAATG 
TGTCCTTTTG ATGTAACAAT GCAAAAGGCC ATCAGTTATT TTGAAATATT TCTTCCTACC 
TCCCTTCCTC CAGAACTGCA TCATAAAGGT TTTAAACTTT GGTTTGATGA ATTAATTGGC 
CTTTGGGTTT CAGTGCAAAA TCTCCCACAG TGGGAGGGGC AATTAGTAAA TCTCTTTGCT 
CGATTGGCTA CAGATAATAT AGGATACATA GATTGGGATC CATATGTACC GAAGATATTT 
ACAAGAATTC TGAGAAGCCT GAACCTCCCA GTGGGAAGCA GTCAAGTTTT AGTCCCAAGA 
TTTTTAACAA ATGCTTATGA TATAGGACAT GCTGTAATAT GGATCACCGC AATGATGGGT 
GGACCAAGTA AATTAGTACA AAAACATTTA GCTGGTTTGT TTAACAGCAT CACATCATTT 
TATCATCCTT CAAATAATGG GCGTTGGCTG AACAAGTTAA TGAAACTCCT TCAGCGGTTG 
CCAAACAGTG TTGTTAGGAG ATTACATCGT GAAAGATTCA AGAAGCCCTC CTGGTTAACT 
CCTGTGCCTG AAAGCCATAA GCTTACTGAT GAAGATGTTA CAGACTTTGT ACAATGCATT 
ATTCAGCCTG TCCTCTTGGC TATGTTTAGC AAAACTGGTA GTCTAGAAGC AGCCCAGGCT 
CTGCAGAACC TTGCACTTAT GAGACCTGAG TTAGTAATAC CCCCTGTGCT TGAAAGAACA 
TACCCTGCTT TAGAGACATT AACAGAACCT CACCAGCTCA CAGCTACTTT AAATTGTGTA 
ATTGGAGTAG CCCGAAGTTT GGTATCAGGC AGCAAATGGT TTCCCGAAGG GCCAACACAC 
ATGCTACCTC TGTTGATGAG AGCATTGCCT GGGGTAGATC CAAACGACTT TAGTAAGTGC 
ATGATCACAT TCCAATTTAT AGGAACATTT TCTACTCTGG TGCCTTTAGT AGACTGTTCA 
TCTGTACTGC AAGAAAGAAA TGACCTCACA GAAATTGAAA AAGAACTTTG TTCGGCAACA 
GCTGAATTTG AGGATTTTGT GTTACAATTT ATGGACAGAT GCTTTGGACT AATAGAAAGT 
AGTACATTGG AGCAAACAAG GGAAGAGACA GAAACAGAGA AAATGACTCA TTTGGAGAGT 
TTGGTTGAAT TAGGCTTATC TTCTACGTTT AGTACAATCC TCACACAATG TTCCAAAGAC 
ATATTTATGG TGGCCCTTCA GAAGGTCTTT AACTTTTCTG TTTCACATAT ATTTGAAACA 
AGAGTTGCAG GTCGCATGGT GGCAGACATG TGCCGAGCTG CTGTGAAGTG TTGCCCAGAA 
GAATCCCTGA AGCTGTTTGT TCCCCACTGC TATGGTGTTA TAACTCAGCT TACAATGAAT 
GATGATGTAT TGAATGAAGA AGAGCTAGAC AAGGAGTTGC TGTGGAACCT TCAGCTTCTG 
TCTGAGATTA CTCGTGTAGA CGGGAAGAAG TTACTTCTGT ATAGGGAGCA GCTTGTAAAG 
ATTCTCCAGC GAACCTTACA CCTGACCTGT AAGCAGGGCT ACACGCTGTC TTGCAACCTT 
CTGCATCATC TTCTTCGTTC TACCACACTA ATCTACCCTA CAGAGTACTG CAGTGTTCCG 
GGGGGCTTTA ACAAGCCGCC TTCTGAATAC TTCCCTGTCA AGGACTGGGG TAAGCCTGGA 
GATTTGTGGA ATCTGGGGAT CCAGTGGCAT GTTCCTTCTT CAGAAGAAGT CTCTTTTGCC 
TTCTATTTGT TAGACTCCTT TCTTCAGCCT GAGCTTATCA AACTCCAGTG TTGTGGAGAT 
GGAGAACTTG AAATGTCTAG AGATGATATT CTACAGAGTC TGACTATAGT ACACAGCTGC 
TTGATTGGTT CAGGGAACCT TCTACCTCCA TTGAAAGGAG AGGCAGTTAC TAACTTGGTA 
CCAAGTATGG TCTCCTTAGA GGAAACAAAA TTGTATACCG GACTGGAACA TGACCTATCC 
AGAGAAAACT ACCGAGAAGT AATTGCTAGT GTCATAAGGA AACTTCTTAG CCACATACTT 
GATAATTCAG AAGATGATAC CAAATCACTG TTTCTCATCA TAAAGATCAT TGGAGACCTT 
CTGCACTTCC AAGGATCTCA TAAACATGAA TTTGACTCTC GATGGAAAAG CTTTAACTTA 
GTAAAGAAAT CAATGGAAAA TCGGCTCCAT GGAAAAAAGC AACATATCAG AGCATTACTA 
ATTGACAGAG TGATGCTGCA GCATGAGTTG CGAACACTAA CTGTTGAGGG TTGTGAGTAC 
AAAAAAATAC ATCAAGATAT GATCAGAGAT CTTCTTCGAT TATCAACAAG CTCATACAGT 
CAGGTCAGAA ACAAAGCTCA GCAAACATTT TTTGCTGCTT TGGGGGCATA TAACTTCTGT 
TGCAGAGATA TTATCCCCTT GGTTTTGGAA TTCCTACGGC CTGATAGAAA GGATGTCACA 
CAGCAGCAGT TCAAGGGCGC CTTATATTGT CTACTTGGAA ATCACAGTGG TGTGTGCCTG 
GCAAACCTTC ATGATTGGGA CTGTATTGTG CAGACCTGGC CAGCACTTGT TTCTTCAGGG 
CTTAGCCAAG CAATGTCACT GGAAAAGCCA TCAATAGTGA GACTATTTGA TGACCTTGCT 
GAAAAGATTC ATAGACAATA TGAAACAATT GGCTTGGATT TTACAATTCC AAAGTCATGT 
GCTGCAATAG CAGAATTACT TCAACAATCA AAGAATCCTT CCATTAGCCA GACACTACTT 
AGCCCAGAAA AGATTAAAGA AGGACAGAAG CGTCAACAAG ATAAGAATGC TGATGCCCTA 
AGGAACTATG AATGTTTGGT AAATACTTTA CTAGATGGTG TGGAGCAAAG AAATCTGCCT 
TGGAAATTTG AGCATATAGG AATCGGGCTT CTGTCTCTAC TCCTAAGGGA TGACAGAGTG 
TTGCCTCTTC GTGCCATACG TTTTTTTGTT GAAAATCTCA ACCATGATGC AATTGTTGTT 
CGAAAGATGG CTATCTCAGC TGTTGCTGGT ATTCTCAAGC AGCTAAAGAG AACTCACAAA 
AAGCTGACTA TCAACCCTTA TGAAATCAGT GGATGTCCTA AACCTACCAA AATTCTTGCT 
GGTGATAGAC CTGATAACCA TTGGTTGCAT TATGATAGTA AAAATATACC AAGAACAAAA 
AAAGAATGGG AGTCAAGTTG CTTTGTTGAA AAAACTCACT GGGGATACTA CAACTGGCCA 
AAGAATATGG TTGTTTATGC TGGTGTGGAA GAACAGCCTA AACTTGGCAG AAGCAGGGAG 
GATATGATAG AGGCAGAACA GATTATATAT GATCGTTTTT CTGATCCTAA GTTTGTTGAG 
CAGTTAATCA CATTTCTATC TTTAGAAGAC AGAAAAGGAA GAGATAAGTT TAGTCCCCGG 
CGTTTCTGCC TCTTTAAGGG TATATTTAGA AATTTTGATG ATGCTTTTCT GCCTGTTCTG 
AAGCCCCATT TAGAACGTTT GGTTGCAGAT TCTCATGAAA GTACCCAGAG ATGTGTTGCA 
GAAATTATAG CTGGTTTAAT CAGAGGTTCT AAGCATTGGA CGTTTGAGAA GGTGGAGAAG 
CTTTGGGAGC TCCTGTGCCC GCTGCTGAGA ACGGCACTGT CCAACATGAC AGTGGAGACA 
TACAATGACT GGGGGACCTG TATAGCCACT TCCTGTGAAA GCAGAGATCC CCGGAAGCTT 
CACTGGCTTT TTGAGCTGCT CTTGGAGTCA CCATTGAGTG GTGAAGGGGG GTCCTTTGTA 
GATGCATGCC GACTCTATGT GCTACAAGGT GGCCTTGCCC AGCAAGAGTG GAGAGTCCCT 
GAACTATTGC ACAGACTTCT GAAGTACTTG GAACCAAAAC TCACCCAGGT TTACAAAAAT 
GTCAGAGAAA GAATAGGAAG TGTGCTGACC TACATATTTA TGATAGATGT TTCTTTGCCA 
AATACTGCTC CAACAACATC TCCTTGTATC CCTGAGTTTA CTGCTCGCGT CCTAGAAAAA 
CTGAAACCAC TCACAGATGT GGATGAAGAA ATTCAGAACC ATGTTATGGA AGAAAATGGC 
ATTGGTGAAG AAGATGAACG AACTCAGGGC ATTAAACTCT TAAAAACTAT ATTGAAATGG 
CTTATGGCAA GTGCAGGAAG ATCTTTTTCT ACAGCAGTCA AAGAGCAACT TCAGCTTCTG 
CCTTTGTTCT TTAAGCTGTC TTCAGACATA CCAGAAGAAG GCATCGGATT CTATTATAGA 
TCACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteasome activators, PA28? and PA200, play indispensable roles in male fertility.
Scientific reports623171(2016 Mar)
Huang L,Haratake K,Miyahara H,Chiba T

JMJD1C Exhibits Multiple Functions in Epigenetic Regulation during Spermatogenesis.
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Acetylation-mediated proteasomal degradation of core histones during DNA repair and spermatogenesis.
Cell153(5)1012-24(2013 May)
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In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
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Differential roles of proteasome and immunoproteasome regulators Pa28??, Pa28? and Pa200 in the degradation of oxidized proteins.
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Pickering AM,Davies KJ

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Psme4 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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