Fig4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fig4
Entrez Gene ID	103199
Full Name	FIG4 phosphoinositide 5-phosphatase
Synonyms	A530089I17Rik,AI326867,Sac3
General protein information	Preferred Names FIG4 phosphoinositide 5-phosphatase Names polyphosphoinositide phosphatase FIG4 homolog SAC domain-containing protein 3 Sac domain-containing inositol phosphatase 3 phosphatidylinositol 3,5-bisphosphate 5-phosphatase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_133999.1	NP_598760.1	polyphosphoinositide phosphatase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FIG4	NP_001108096.1
Canis lupus familiaris (dog)	FIG4	NP_001108097.1
Bos taurus (cattle)	FIG4	NP_001069482.1
Saccharomyces cerevisiae (baker's yeast)	FIG4	NP_014074.1
Schizosaccharomyces pombe (fission yeast)	SPAC1093.03	NP_001018284.3
Magnaporthe oryzae (rice blast fungus)	MGG_07091	XP_003715282.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004477	XP_313776.4
Mus musculus (house mouse)	Fig4	NP_598760.1
Rattus norvegicus (Norway rat)	Fig4	NP_001040561.1
Gallus gallus (chicken)	FIG4	NP_001108095.1
Drosophila melanogaster (fruit fly)	CG17840	NP_608841.1
Eremothecium gossypii	AGOS_ADL195C	NP_983901.2
Xenopus tropicalis (tropical clawed frog)	fig4	XP_004914917.1
Homo sapiens (human)	FIG4	NP_055660.1
Danio rerio (zebrafish)	fig4	XP_002667265.3
Kluyveromyces lactis	KLLA0E02113g	XP_454043.1
Neurospora crassa	NCU08689	XP_961768.2
	FIG4	NP_001165904.1

Gene Ontology
Bibliography

Related articles in PubMed

PI(3,5)P2 biosynthesis regulates oligodendrocyte differentiation by intrinsic and extrinsic mechanisms.
Mironova YA, Lenk GM, Lin JP, Lee SJ, Twiss JL, Vaccari I, Bolino A, Havton LA, Min SH, Abrams CS, Shrager P, Meisler MH, Giger RJ
eLife5(2016 03)

Rescue of neurodegeneration in the Fig4 null mouse by a catalytically inactive FIG4 transgene.
Lenk GM, Frei CM, Miller AC, Wallen RC, Mironova YA, Giger RJ, Meisler MH
Human molecular genetics25(2)340-7(2016 Jan)

Loss of Fig4 in both Schwann cells and motor neurons contributes to CMT4J neuropathy.
Vaccari I, Carbone A, Previtali SC, Mironova YA, Alberizzi V, Noseda R, Rivellini C, Bianchi F, Del Carro U, D'Antonio M, Lenk GM, Wrabetz L, Giger RJ, Meisler MH, Bolino A
Human molecular genetics24(2)383-96(2015 Jan)

Jab1 regulates Schwann cell proliferation and axonal sorting through p27.
Porrello E, Rivellini C, Dina G, Triolo D, Del Carro U, Ungaro D, Panattoni M, Feltri ML, Wrabetz L, Pardi R, Quattrini A, Previtali SC
The Journal of experimental medicine211(1)29-43(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Neuronal expression of Fig4(C486S) transgene prevents neonatal neurodegeneration & juvenile lethality seen in Fig4-/- mice. However, later in life Fig4(C486S) causes significant abnormalities: hydrocephalus, defective myelination &reduced lifespan
Title: Rescue of neurodegeneration in the Fig4 null mouse by a catalytically inactive FIG4 transgene.

Inactivation of Fig4 in motor neurons display neuronal and axonal degeneration.
Title: Loss of Fig4 in both Schwann cells and motor neurons contributes to CMT4J neuropathy.

Fig4 expression in the neurons is both necessary and sufficient to prevent spongiform neurodegeneration
Title: Neuronal expression of Fig4 is both necessary and sufficient to prevent spongiform neurodegeneration.

Fig4 may be required to prevent neurons and glia from excessive lysosomal accumulation after injury and in neurodegeneration.
Title: Fig4 expression in the rodent nervous system and its potential role in preventing abnormal lysosomal accumulation.

Here we provide strong evidence that Mtmr2 and Fig4 functionally interact in both Schwann cells and neurons, and we reveal for the first time a role of Mtmr2 in neurons in vivo
Title: Genetic interaction between MTMR2 and FIG4 phospholipid phosphatases involved in Charcot-Marie-Tooth neuropathies.

The following Fig4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fig4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22291	NM_133999.1 Latest version!	Mus musculus FIG4 phosphoinositide 5-phosphatase (Fig4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fig4 ( NM_133999.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598760.1 Mus musculus FIG4 phosphoinositide 5-phosphatase (Fig4), mRNA.

Price & Availability↑

CloneID	OMu22291
Clone ID Related Accession (Same CDS sequence)	NM_133999.1
Accession Version	NM_133999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	polyphosphoinositide phosphatase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC015295.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015295.1, AK041196.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCACGG CCGCTGCCCC CATCATCAGC TCGGTCCAGA AGCTGGTGCT GTATGAGACC 
AGAGCTAGAT ACTTTCTAGT TGGGAGCAAT CATGCAGAAA CGAAGTACCG TGTCCTGAAA 
ATTGATAGAA CAGAACCGAA GGACTTGGTG GTAATCGACG ACAGGCACGT GTACACACAA 
CAAGAAGTGA GGGAACTTCT TGGCCGCCTG GATCTGGGAA ACAGAACAAA GATGAGCCAG 
AAAGGATCCT CCGGGTTGTT TCGAGCCGTC TCAGCTTTTG GAGTCGTAGG TTTTGTCAGG 
TTCTTAGAAG GCTACTATAT TGTATTAATA ACTAAGCGGA GGAAGATGGC AGACATTGGA 
GGTCATGCAA TTTATAAGAT TGAAGATACA AGTATGATTT ATATCCCCAA TGACTCTGTT 
CGGATTTCTC ACCCCGACGA AGCTAGGTAT CTAAGGATAT TTCAAAATGT GGATCTATCT 
AGCAATTTTT ACTTTAGTTA CAGCTATGAT TTGTCACACT CACTTCAGTA TAATCTTACC 
GTCCTGCGAA TGCCCCTGGA GATGTTAAAG TCAGAAACAT CCAAGGCGTG CCAGGAGAGC 
TTCGACATCT TTGAAGATGA AGGATTAATT ACACAGGGTG GCAGTGGTGT ATTTGGGATC 
TCTAGTGAGC CTTATATGAA GTATGTATGG AACGGTGAAC TTCTGGATAT AATTAAAAAC 
ACTGTGCATC GTGACTGGCT ATTGTATATC ATTCATGGGT TCTGCGGGCA GTCCAAGCTT 
TTGATCTATG GACGACCAGT GTATGTGACC CTAATAGCCA GGAGATCCAG TAGGTTTGCT 
GGGACGCGCT TTCTCAAGAG AGGCGCAAAC TGTGAGGGTG ACGTTGCAAA TGAGGTGGAG 
ACCGAGCAGA TCCTCTGTGA TGCTTCTGTG ATGTCTTTTA CCGCAGGCAG TTACTCTTCT 
TACGTACAAG TTAGAGGATC CGTTCCCTTA TTCTGGTCCC AAGACATCTC AACTATGATG 
CCGAAACCAC CCATTACACT GGACCAGGCG GATCCCTTTG CACACGTGGC TGCTCTTCAC 
TTTGACCAGA TGCTCCAGAG GTTCGGCTCC CCCATCATCA TCTTGAACTT AGTGAAGGAG 
AGGGAGAAGA GAAAGCACGA AAGGATCCTG AGTGAAGAGC TGGTGGCTGC GGTCACTTAC 
CTGAACCAGT TCCTGCCACC GGAGCACACC ATTGTCTACA TCCCCTGGGA CATGGCCAAG 
TACACCAAGA GTAAGCTGTG CAACGTTCTC GATCGGCTGA ATGTGATTGC GGAAAGCGTG 
GTAAAGAAGA CAGGATTCTT TGTAAACCGC CCTGATTCTT ACTGCAGCAT TTTACGGCCA 
GATGAAAAGT GGAATGAACT AGGAGGTCAT GTGATTCCCA CTGGTCGGCT CCAGACTGGC 
ATCCTTCGAA CCAACTGTGT GGACTGTTTG GATCGCACCA ACACTGCGCA GTTCATGGTG 
GGCAAGTGTG CCCTGGCTTA CCAGCTGTAT TCCTTAGGGC TGATTGACAA GCCTAACCTG 
CAGTTCGACA CGGATGCAGT GAGGTTGTTT GAGGAACTCT ATGAAGACCA TGGAGACACC 
CTTTCCCTGC AGTACGGAGG CTCTCAGCTG GTTCATCGGG TAAAGACCTA CAGAAAGATA 
GCACCGTGGA CCCAGCACTC CAAGGACATC ATGCAGACTC TGTCCCGGTA CTACAGCAAC 
GCCTTCTCGG ATGCTGATAG ACAAGATTCC ATCAACCTGT TTTTGGGTGT TTTCCACCCC 
ACAGAAGGGA AACCTCATCT CTGGGAACTC CCCACAGACT TTTACCTCCA TCACAAAAAC 
ACCATGAGCC TTTTACCCCC CAGAAGGAGT TATACTTACT GGTGGACGCC AGAGGTGGTG 
AAGCATCTGC CGCTGCCTTA CGATGAAGTT ATCTGTGCTG CAAACTTAAA GAAGTTGATG 
GTGAAGAAAT TTCACAGATG GGAAGAAGAG ATTGATATTC ACAATGAGTT CTTCCGGCCG 
TATGAATTGA GTAGTTTTGA TGACACCTTT TGCTTGGCCA TGACAAGTTC AGCACGTGAT 
TTTATGCCTA AAACTGTTGG CATTGATCCA AGTCCATTTA CCGTGCGAAA ACCAGATGAA 
ACTGGAAAAT CTGTACTGGG GAACAAAAAC ACTAGAGAGG AAGCTGTCCT GCAGCGGAAG 
ACGGCAGCCA GCGCCCCGCC ACCCCCGAGC GAGGAGGCTG TGTCCAGCAG CTCTGAGGAT 
GACTCTGGCA CCGACCGGGA AGATGAAGGC TCCATCTCTC AGCGATCCAC TCCTGTGAAG 
ATGACTGACA CGGGAGACAG TGCCAAAGCG ACTGAGAATG TGGTCCAGCC CATGAAGGAA 
GTGTATGGAG TCAGTCTCTC CAGTAGCCTG TCAGAGGAGG ATCACTCCAT TTATGCCAGG 
TTTGTTCAGC TGGGGCAAAG TCAACATAAA CAGGACCGAG GTAACCAGCA GCTGTGTTCC 
CGTTGCTCAG ATGGAGTTAT AAAACTAACT CCCATCTCAG CTTTCTCGCA AGACAACATT 
TATGAAGTTC AGCCTCCAAG AGTAGACAGA AAGTCTACAG AGATCTTCCA GGCCCATATC 
CAAGCTAGCC AAGGTATCAT GCAGCCCCTT GGCAAAGAGG ACACTGCCAT GTACCGGGAG 
TACATCAGAA ACCGCTACTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598760.1
CDS	190..2913
Translation	MPTAAAPIISSVQKLVLYETRARYFLVGSNHAETKYRVLKIDRTEPKDLVVIDDRHVYTQQEVRELLGRLDLGNRTKMSQKGSSGLFRAVSAFGVVGFVRFLEGYYIVLITKRRKMADIGGHAIYKIEDTSMIYIPNDSVRISHPDEARYLRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEMLKSETSKACQESFDIFEDEGLITQGGSGVFGISSEPYMKYVWNGELLDIIKNTVHRDWLLYIIHGFCGQSKLLIYGRPVYVTLIARRSSRFAGTRFLKRGANCEGDVANEVETEQILCDASVMSFTAGSYSSYVQVRGSVPLFWSQDISTMMPKPPITLDQADPFAHVAALHFDQMLQRFGSPIIILNLVKEREKRKHERILSEELVAAVTYLNQFLPPEHTIVYIPWDMAKYTKSKLCNVLDRLNVIAESVVKKTGFFVNRPDSYCSILRPDEKWNELGGHVIPTGRLQTGILRTNCVDCLDRTNTAQFMVGKCALAYQLYSLGLIDKPNLQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDSINLFLGVFHPTEGKPHLWELPTDFYLHHKNTMSLLPPRRSYTYWWTPEVVKHLPLPYDEVICAANLKKLMVKKFHRWEEEIDIHNEFFRPYELSSFDDTFCLAMTSSARDFMPKTVGIDPSPFTVRKPDETGKSVLGNKNTREEAVLQRKTAASAPPPPSEEAVSSSSEDDSGTDREDEGSISQRSTPVKMTDTGDSAKATENVVQPMKEVYGVSLSSSLSEEDHSIYARFVQLGQSQHKQDRGNQQLCSRCSDGVIKLTPISAFSQDNIYEVQPPRVDRKSTEIFQAHIQASQGIMQPLGKEDTAMYREYIRNRYL

Target ORF information:

RefSeq Version	NM_133999.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus FIG4 phosphoinositide 5-phosphatase (Fig4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133999.1

ORF Insert Sequence:

ATGCCCACGG CCGCTGCCCC CATCATCAGC TCGGTCCAGA AGCTGGTGCT GTATGAGACC 
AGAGCTAGAT ACTTTCTAGT TGGGAGCAAT CATGCAGAAA CGAAGTACCG TGTCCTGAAA 
ATTGATAGAA CAGAACCGAA GGACTTGGTG GTAATCGACG ACAGGCACGT GTACACACAA 
CAAGAAGTGA GGGAACTTCT TGGCCGCCTG GATCTGGGAA ACAGAACAAA GATGAGCCAG 
AAAGGATCCT CCGGGTTGTT TCGAGCCGTC TCAGCTTTTG GAGTCGTAGG TTTTGTCAGG 
TTCTTAGAAG GCTACTATAT TGTATTAATA ACTAAGCGGA GGAAGATGGC AGACATTGGA 
GGTCATGCAA TTTATAAGAT TGAAGATACA AGTATGATTT ATATCCCCAA TGACTCTGTT 
CGGATTTCTC ACCCCGACGA AGCTAGGTAT CTAAGGATAT TTCAAAATGT GGATCTATCT 
AGCAATTTTT ACTTTAGTTA CAGCTATGAT TTGTCACACT CACTTCAGTA TAATCTTACC 
GTCCTGCGAA TGCCCCTGGA GATGTTAAAG TCAGAAACAT CCAAGGCGTG CCAGGAGAGC 
TTCGACATCT TTGAAGATGA AGGATTAATT ACACAGGGTG GCAGTGGTGT ATTTGGGATC 
TCTAGTGAGC CTTATATGAA GTATGTATGG AACGGTGAAC TTCTGGATAT AATTAAAAAC 
ACTGTGCATC GTGACTGGCT ATTGTATATC ATTCATGGGT TCTGCGGGCA GTCCAAGCTT 
TTGATCTATG GACGACCAGT GTATGTGACC CTAATAGCCA GGAGATCCAG TAGGTTTGCT 
GGGACGCGCT TTCTCAAGAG AGGCGCAAAC TGTGAGGGTG ACGTTGCAAA TGAGGTGGAG 
ACCGAGCAGA TCCTCTGTGA TGCTTCTGTG ATGTCTTTTA CCGCAGGCAG TTACTCTTCT 
TACGTACAAG TTAGAGGATC CGTTCCCTTA TTCTGGTCCC AAGACATCTC AACTATGATG 
CCGAAACCAC CCATTACACT GGACCAGGCG GATCCCTTTG CACACGTGGC TGCTCTTCAC 
TTTGACCAGA TGCTCCAGAG GTTCGGCTCC CCCATCATCA TCTTGAACTT AGTGAAGGAG 
AGGGAGAAGA GAAAGCACGA AAGGATCCTG AGTGAAGAGC TGGTGGCTGC GGTCACTTAC 
CTGAACCAGT TCCTGCCACC GGAGCACACC ATTGTCTACA TCCCCTGGGA CATGGCCAAG 
TACACCAAGA GTAAGCTGTG CAACGTTCTC GATCGGCTGA ATGTGATTGC GGAAAGCGTG 
GTAAAGAAGA CAGGATTCTT TGTAAACCGC CCTGATTCTT ACTGCAGCAT TTTACGGCCA 
GATGAAAAGT GGAATGAACT AGGAGGTCAT GTGATTCCCA CTGGTCGGCT CCAGACTGGC 
ATCCTTCGAA CCAACTGTGT GGACTGTTTG GATCGCACCA ACACTGCGCA GTTCATGGTG 
GGCAAGTGTG CCCTGGCTTA CCAGCTGTAT TCCTTAGGGC TGATTGACAA GCCTAACCTG 
CAGTTCGACA CGGATGCAGT GAGGTTGTTT GAGGAACTCT ATGAAGACCA TGGAGACACC 
CTTTCCCTGC AGTACGGAGG CTCTCAGCTG GTTCATCGGG TAAAGACCTA CAGAAAGATA 
GCACCGTGGA CCCAGCACTC CAAGGACATC ATGCAGACTC TGTCCCGGTA CTACAGCAAC 
GCCTTCTCGG ATGCTGATAG ACAAGATTCC ATCAACCTGT TTTTGGGTGT TTTCCACCCC 
ACAGAAGGGA AACCTCATCT CTGGGAACTC CCCACAGACT TTTACCTCCA TCACAAAAAC 
ACCATGAGCC TTTTACCCCC CAGAAGGAGT TATACTTACT GGTGGACGCC AGAGGTGGTG 
AAGCATCTGC CGCTGCCTTA CGATGAAGTT ATCTGTGCTG CAAACTTAAA GAAGTTGATG 
GTGAAGAAAT TTCACAGATG GGAAGAAGAG ATTGATATTC ACAATGAGTT CTTCCGGCCG 
TATGAATTGA GTAGTTTTGA TGACACCTTT TGCTTGGCCA TGACAAGTTC AGCACGTGAT 
TTTATGCCTA AAACTGTTGG CATTGATCCA AGTCCATTTA CCGTGCGAAA ACCAGATGAA 
ACTGGAAAAT CTGTACTGGG GAACAAAAAC ACTAGAGAGG AAGCTGTCCT GCAGCGGAAG 
ACGGCAGCCA GCGCCCCGCC ACCCCCGAGC GAGGAGGCTG TGTCCAGCAG CTCTGAGGAT 
GACTCTGGCA CCGACCGGGA AGATGAAGGC TCCATCTCTC AGCGATCCAC TCCTGTGAAG 
ATGACTGACA CGGGAGACAG TGCCAAAGCG ACTGAGAATG TGGTCCAGCC CATGAAGGAA 
GTGTATGGAG TCAGTCTCTC CAGTAGCCTG TCAGAGGAGG ATCACTCCAT TTATGCCAGG 
TTTGTTCAGC TGGGGCAAAG TCAACATAAA CAGGACCGAG GTAACCAGCA GCTGTGTTCC 
CGTTGCTCAG ATGGAGTTAT AAAACTAACT CCCATCTCAG CTTTCTCGCA AGACAACATT 
TATGAAGTTC AGCCTCCAAG AGTAGACAGA AAGTCTACAG AGATCTTCCA GGCCCATATC 
CAAGCTAGCC AAGGTATCAT GCAGCCCCTT GGCAAAGAGG ACACTGCCAT GTACCGGGAG 
TACATCAGAA ACCGCTACTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PI(3,5)P2 biosynthesis regulates oligodendrocyte differentiation by intrinsic and extrinsic mechanisms.
eLife5(2016 03)
Mironova YA,Lenk GM,Lin JP,Lee SJ,Twiss JL,Vaccari I,Bolino A,Havton LA,Min SH,Abrams CS,Shrager P,Meisler MH,Giger RJ

Rescue of neurodegeneration in the Fig4 null mouse by a catalytically inactive FIG4 transgene.
Human molecular genetics25(2)340-7(2016 Jan)
Lenk GM,Frei CM,Miller AC,Wallen RC,Mironova YA,Giger RJ,Meisler MH

Loss of Fig4 in both Schwann cells and motor neurons contributes to CMT4J neuropathy.
Human molecular genetics24(2)383-96(2015 Jan)
Vaccari I,Carbone A,Previtali SC,Mironova YA,Alberizzi V,Noseda R,Rivellini C,Bianchi F,Del Carro U,D'Antonio M,Lenk GM,Wrabetz L,Giger RJ,Meisler MH,Bolino A

Jab1 regulates Schwann cell proliferation and axonal sorting through p27.
The Journal of experimental medicine211(1)29-43(2014 Jan)
Porrello E,Rivellini C,Dina G,Triolo D,Del Carro U,Ungaro D,Panattoni M,Feltri ML,Wrabetz L,Pardi R,Quattrini A,Previtali SC

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Fig4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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