Chchd10 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chchd10
Entrez Gene ID	103172
Full Name	coiled-coil-helix-coiled-coil-helix domain containing 10
Synonyms	1620401E04Rik,AI267078,Ndg2
General protein information	Preferred Names coiled-coil-helix-coiled-coil-helix domain containing 10 Names coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial Nur77 downstream gene 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_175329.4	NP_780538.2	coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial
XM_006512993.1	XP_006513056.1	coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHCHD10	XP_001162555.1
Canis lupus familiaris (dog)	CHCHD10	XP_534755.3
Gallus gallus (chicken)	LOC416933	XP_003642270.1
Danio rerio (zebrafish)	chchd10	NP_957078.1
Xenopus tropicalis (tropical clawed frog)	chchd10	XP_002940705.1
Homo sapiens (human)	CHCHD10	NP_998885.1
Rattus norvegicus (Norway rat)	Chchd10	NP_001007009.1
Bos taurus (cattle)	CHCHD10	NP_001077243.1
Mus musculus (house mouse)	Chchd10	NP_780538.2

Gene Ontology
Bibliography

Related articles in PubMed

In vitro and in vivo studies of the ALS-FTLD protein CHCHD10 reveal novel mitochondrial topology and protein interactions.
Burstein SR, Valsecchi F, Kawamata H, Bourens M, Zeng R, Zuberi A, Milner TA, Cloonan SM, Lutz C, Barrientos A, Manfredi G
Human molecular genetics27(1)160-177(2018 Jan)

Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity.
Woo JA, Liu T, Trotter C, Fang CC, De Narvaez E, LePochat P, Maslar D, Bukhari A, Zhao X, Deonarine A, Westerheide SD, Kang DE
Nature communications815558(2017 Jun)

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
Tang F, Barbacioru C, Nordman E, Bao S, Lee C, Wang X, Tuch BB, Heard E, Lao K, Surani MA
PloS one6(6)e21208(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nur77 gene knockout alters dopamine neuron biochemical activity and dopamine turnover.
Title: Nur77 gene knockout alters dopamine neuron biochemical activity and dopamine turnover.

In the presence of HA1004, NBRE binding by Nur77 protein increases with time (6 h vs 2 h), whereas the final outcome of both inhibitors is apoptosis of thymic lymphoma cells.
Title: Nur77 nuclear import and its NBRE-binding activity in thymic lymphoma cells are regulated by different mechanisms sensitive to FK506 or HA1004.

The following Chchd10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chchd10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05179	NM_175329.4 Latest version!	Mus musculus coiled-coil-helix-coiled-coil-helix domain containing 10 (Chchd10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu05179	XM_006512993.1 Latest version!	Mus musculus coiled-coil-helix-coiled-coil-helix domain containing 10 (Chchd10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chchd10 ( NM_175329.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780538.2 Mus musculus coiled-coil-helix-coiled-coil-helix domain containing 10 (Chchd10), mRNA.

Price & Availability↑

CloneID	OMu05179
Clone ID Related Accession (Same CDS sequence)	NM_175329.4 , XM_006512993.1
Accession Version	NM_175329.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM934773.1, BC083357.1 and W16247.1. On Feb 8, 2017 this sequence version replaced NM_175329.3. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTCGGG GAAGCCGCAG TGCGGCTGCC AGGCCGGTCA GCCGCCCAGC CCCGCCTCCT 
GCGCACCCAC CACCCTCAGC GGCCGCCCCG GCACCTGCCG CTCCCGGCCA GCCGGGTCTT 
ATGGCTCAGA TGGCATCCAC CGCCGCAGGC GTAGCCGTGG GCTCAGCTGT AGGGCATGTC 
ATGGGTAGCG CCCTGACCAG TGCCTTCAGT GGGGGAAATT CAGAGCCTGC CCAGCCTGCC 
GTCCAGCAGG CCCCTGCCCG GCCTGCCTCT CACCCCCTGC AGATGGGGCC CTGCTCCTAT 
GAGATCAAGC AGTTCCTGGA CTGCTCCACC ACTCAGAGCG ACCTAACCCT GTGTGAGGGC 
TTCAGCGAGG CCCTCAAACA GTGCAAATAC AATCACGGTC TGAGCTCCCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780538.2
CDS	919..1335
Translation	MPRGSRSAAARPVSRPAPPPAHPPPSAAAPAPAAPGQPGLMAQMASTAAGVAVGSAVGHVMGSALTSAFSGGNSEPAQPAVQQAPARPASHPLQMGPCSYEIKQFLDCSTTQSDLTLCEGFSEALKQCKYNHGLSSLP

Target ORF information:

RefSeq Version	NM_175329.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus coiled-coil-helix-coiled-coil-helix domain containing 10 (Chchd10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175329.4

ORF Insert Sequence:

ATGCCTCGGG GAAGCCGCAG TGCGGCTGCC AGGCCGGTCA GCCGCCCAGC CCCGCCTCCT 
GCGCACCCAC CACCCTCAGC GGCCGCCCCG GCACCTGCCG CTCCCGGCCA GCCGGGTCTT 
ATGGCTCAGA TGGCATCCAC CGCCGCAGGC GTAGCCGTGG GCTCAGCTGT AGGGCATGTC 
ATGGGTAGCG CCCTGACCAG TGCCTTCAGT GGGGGAAATT CAGAGCCTGC CCAGCCTGCC 
GTCCAGCAGG CCCCTGCCCG GCCTGCCTCT CACCCCCTGC AGATGGGGCC CTGCTCCTAT 
GAGATCAAGC AGTTCCTGGA CTGCTCCACC ACTCAGAGCG ACCTAACCCT GTGTGAGGGC 
TTCAGCGAGG CCCTCAAACA GTGCAAATAC AATCACGGTC TGAGCTCCCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chchd10 ( XM_006512993.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513056.1 Mus musculus coiled-coil-helix-coiled-coil-helix domain containing 10 (Chchd10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05179
Clone ID Related Accession (Same CDS sequence)	NM_175329.4 , XM_006512993.1
Accession Version	XM_006512993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCGGG GAAGCCGCAG TGCGGCTGCC AGGCCGGTCA GCCGCCCAGC CCCGCCTCCT 
GCGCACCCAC CACCCTCAGC GGCCGCCCCG GCACCTGCCG CTCCCGGCCA GCCGGGTCTT 
ATGGCTCAGA TGGCATCCAC CGCCGCAGGC GTAGCCGTGG GCTCAGCTGT AGGGCATGTC 
ATGGGTAGCG CCCTGACCAG TGCCTTCAGT GGGGGAAATT CAGAGCCTGC CCAGCCTGCC 
GTCCAGCAGG CCCCTGCCCG GCCTGCCTCT CACCCCCTGC AGATGGGGCC CTGCTCCTAT 
GAGATCAAGC AGTTCCTGGA CTGCTCCACC ACTCAGAGCG ACCTAACCCT GTGTGAGGGC 
TTCAGCGAGG CCCTCAAACA GTGCAAATAC AATCACGGTC TGAGCTCCCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513056.1
CDS	1079..1495
Translation	MPRGSRSAAARPVSRPAPPPAHPPPSAAAPAPAAPGQPGLMAQMASTAAGVAVGSAVGHVMGSALTSAFSGGNSEPAQPAVQQAPARPASHPLQMGPCSYEIKQFLDCSTTQSDLTLCEGFSEALKQCKYNHGLSSLP

Target ORF information:

RefSeq Version	XM_006512993.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus coiled-coil-helix-coiled-coil-helix domain containing 10 (Chchd10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512993.1

ORF Insert Sequence:

ATGCCTCGGG GAAGCCGCAG TGCGGCTGCC AGGCCGGTCA GCCGCCCAGC CCCGCCTCCT 
GCGCACCCAC CACCCTCAGC GGCCGCCCCG GCACCTGCCG CTCCCGGCCA GCCGGGTCTT 
ATGGCTCAGA TGGCATCCAC CGCCGCAGGC GTAGCCGTGG GCTCAGCTGT AGGGCATGTC 
ATGGGTAGCG CCCTGACCAG TGCCTTCAGT GGGGGAAATT CAGAGCCTGC CCAGCCTGCC 
GTCCAGCAGG CCCCTGCCCG GCCTGCCTCT CACCCCCTGC AGATGGGGCC CTGCTCCTAT 
GAGATCAAGC AGTTCCTGGA CTGCTCCACC ACTCAGAGCG ACCTAACCCT GTGTGAGGGC 
TTCAGCGAGG CCCTCAAACA GTGCAAATAC AATCACGGTC TGAGCTCCCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In vitro and in vivo studies of the ALS-FTLD protein CHCHD10 reveal novel mitochondrial topology and protein interactions.
Human molecular genetics27(1)160-177(2018 Jan)
Burstein SR,Valsecchi F,Kawamata H,Bourens M,Zeng R,Zuberi A,Milner TA,Cloonan SM,Lutz C,Barrientos A,Manfredi G

Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity.
Nature communications815558(2017 Jun)
Woo JA,Liu T,Trotter C,Fang CC,De Narvaez E,LePochat P,Maslar D,Bukhari A,Zhao X,Deonarine A,Westerheide SD,Kang DE

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.
PloS one6(6)e21208(2011)
Tang F,Barbacioru C,Nordman E,Bao S,Lee C,Wang X,Tuch BB,Heard E,Lao K,Surani MA

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Chchd10 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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