Ppp1r8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp1r8
Entrez Gene ID	100336
Full Name	protein phosphatase 1, regulatory (inhibitor) subunit 8
Synonyms	6330548N22Rik,AU044684,NIPP1
General protein information	Preferred Names protein phosphatase 1, regulatory (inhibitor) subunit 8 Names nuclear inhibitor of protein phosphatase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006538464.3	XP_006538527.2	nuclear inhibitor of protein phosphatase 1 isoform X1
XM_006538465.3	XP_006538528.2	nuclear inhibitor of protein phosphatase 1 isoform X2
NM_001355199.1	NP_001342128.1	nuclear inhibitor of protein phosphatase 1 isoform 4
NM_146154.3	NP_666266.1	nuclear inhibitor of protein phosphatase 1 isoform 1
NM_001355198.1	NP_001342127.1	nuclear inhibitor of protein phosphatase 1 isoform 3
NM_001290725.1	NP_001277654.1	nuclear inhibitor of protein phosphatase 1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPP1R8	NP_054829.2
	PPP1R8	XP_001112323.1
Bos taurus (cattle)	PPP1R8	NP_777007.1
Caenorhabditis elegans (roundworm)	B0511.7	NP_492781.2
Mus musculus (house mouse)	Ppp1r8	NP_666266.1
Gallus gallus (chicken)	PPP1R8	NP_001026062.1
Pan troglodytes (chimpanzee)	PPP1R8	XP_524632.2
Drosophila melanogaster (fruit fly)	NiPp1	NP_611177.1
Xenopus tropicalis (tropical clawed frog)	ppp1r8	NP_001017079.1
Canis lupus familiaris (dog)	PPP1R8	XP_544466.2
Rattus norvegicus (Norway rat)	Ppp1r8	NP_001101381.1
Danio rerio (zebrafish)	ppp1r8a	NP_001094422.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012089	XP_320437.4

Gene Ontology
Bibliography

Related articles in PubMed

The protein phosphatase 1 regulator NIPP1 is essential for mammalian spermatogenesis.
Ferreira M, Boens S, Winkler C, Szek?r K, Verbinnen I, Van Eynde A, Fardilha M, Bollen M
Scientific reports7(1)13364(2017 Oct)

Brief Report: The Deletion of the Phosphatase Regulator NIPP1 Causes Progenitor Cell Expansion in the Adult Liver.
Boens S, Verbinnen I, Verhulst S, Szek?r K, Ferreira M, Gevaert T, Baes M, Roskams T, van Grunsven LA, Van Eynde A, Bollen M
Stem cells (Dayton, Ohio)34(8)2256-62(2016 08)

BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes.
Yu C, Ji SY, Sha QQ, Dang Y, Zhou JJ, Zhang YL, Liu Y, Wang ZW, Hu B, Sun QY, Sun SC, Tang F, Fan HY
Nature structural & molecular biology23(5)387-94(2016 05)

An AUTS2-Polycomb complex activates gene expression in the CNS.
Gao Z, Lee P, Stafford JM, von Schimmelmann M, Schaefer A, Reinberg D
Nature516(7531)349-54(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NIPP1 does not regulate the proliferation of hepatocytes but is a suppressor of biliary epithelial cell proliferation, including progenitor cells, in the adult liver.
Title: Brief Report: The Deletion of the Phosphatase Regulator NIPP1 Causes Progenitor Cell Expansion in the Adult Liver.

activation of the RAGE by advanced glycation end products or other ligands suppresses NIPP1 expression in diabetic nephropathy, contributes to podocyte hypertrophy, and glomerular inflammation
Title: Activation of the receptor for advanced glycation end products induces nuclear inhibitor of protein phosphatase-1 suppression.

Required for the global trimethylation of Histone 3 at Lysine 27 and is implicated in gene silencing by enhancer of zeste homolog (EZH)2.
Title: The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing.

NIPP1 has a role in the nuclear targeting and/or retention of PP1
Title: Interactor-mediated nuclear translocation and retention of protein phosphatase-1.

NIPP1(-/-) embryos showed severely retarded growth at embryonic day 6.5 (E6.5) and were resorbed by E8.5. This early embryonic lethality was not associated with increased apoptosis but correlated with impaired cell proliferation.
Title: The nuclear scaffold protein NIPP1 is essential for early embryonic development and cell proliferation.

The following Ppp1r8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp1r8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16794	NM_001290725.1 Latest version!	Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu72001	NM_001355198.1 Latest version!	Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu72002	NM_001355199.1 Latest version!	Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu72001	XM_006538464.3 Latest version!	Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 8 (Ppp1r8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu72002	XM_006538465.3 Latest version!	Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 8 (Ppp1r8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu14284	NM_146154.3 Latest version!	Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp1r8 ( NM_001290725.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277654.1 Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16794
Clone ID Related Accession (Same CDS sequence)	NM_001290725.1
Accession Version	NM_001290725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	nuclear inhibitor of protein phosphatase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY293277.1, BY257668.1, CK625884.1, AK032022.1 and BQ749177.1. On Mar 19, 2014 this sequence version replaced XM_006538466.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter by an amino acid, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ895084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CCGTGAACTC CGGCTCCAGC CTCCCGCTGT TCGACTGCCC GACTTGGGCA 
GGTAAACCAC CACCTGGCTT ACATCTGGAT GTGGTGAAAG GAGACAAGCT AATTGAGAAG 
CTGATTATTG ATGAGAAAAA GTATTACTTA TTTGGGAGGA ACCCCGATCT GTGTGACTTC 
ACTATCGACC ACCAGTCATG CTCTCGAGTC CACGCAGCGC TGGTGTACCA CAAACACCTG 
AAGAGAGTTT TCTTGATAGA CCTCAACAGC ACACATGGCA CTTTCTTGGG TCACATTCGG 
CTGGAACCTC ACAAGCCTCA ACAGATTCCC ATCGATTCTA CGGTCTCATT TGGCGCGTCC 
ACGAGGGCAT ACACTCTGCG AGAGAAGCCT CAGACATTGC CATCGGCTGT GAAAGGAGAT 
GAGAAGATGG GCGGAGAGGA TGATGAACTC AAGGGCTTGT TGGGACTTCC CGAAGAGGAG 
ACCGAGCTGG ATAACCTGAC AGAGTTCAAC ACTGCCCACA ACAAGCGGAT TTCAACCCTC 
ACTATTGAGG AGGGGAACCT GGACATCCAG AGACCAAAGA GGAAGAGGAA GAACTCAAGG 
GTGACCTTCA GTGAGGATGA CGAGATCATC AACCCAGAGG ACGTGGATCC GTCTGTTGGT 
CGGTTCCGGA ACATGGTGCA GACAGCAGTG GTTCCAGTCA AGAAGCGGAT GGAGGGCTCT 
GGCTCTCTGG GCCTGGAGGA GTCGGGGAGC AGGCGCATGC AGAACTTTGC CTTCAGTGGA 
GGACTATATG GGGGCCTGCC CCCCACACAC AGTGAAACGG GCTCCCAGCC GCATGGCATT 
CATGGGACAG CGCTCATTGG TGGCTTGCCC ATGCCATACC CGAACCTCGC CCCTGATGTG 
GACTTGACTC CTGTTGTGCC ATCAGCAGTG GCCATAAATC CCACACCAAA CCCTGCAGTC 
TATAACCCTG AGGCTGTGAA TGAACCCAAG AAGAAGAAAT ACGCGAAGGA GGCTTGGCCG 
GGCAAGAAGC CCACACCTTC CTTACTGATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277654.1
CDS	58..1110
Translation	MAAAVNSGSSLPLFDCPTWAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGDEKMGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKRMEGSGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSETGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVAINPTPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

Target ORF information:

RefSeq Version	NM_001290725.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290725.1

ORF Insert Sequence:

ATGGCGGCAG CCGTGAACTC CGGCTCCAGC CTCCCGCTGT TCGACTGCCC GACTTGGGCA 
GGTAAACCAC CACCTGGCTT ACATCTGGAT GTGGTGAAAG GAGACAAGCT AATTGAGAAG 
CTGATTATTG ATGAGAAAAA GTATTACTTA TTTGGGAGGA ACCCCGATCT GTGTGACTTC 
ACTATCGACC ACCAGTCATG CTCTCGAGTC CACGCAGCGC TGGTGTACCA CAAACACCTG 
AAGAGAGTTT TCTTGATAGA CCTCAACAGC ACACATGGCA CTTTCTTGGG TCACATTCGG 
CTGGAACCTC ACAAGCCTCA ACAGATTCCC ATCGATTCTA CGGTCTCATT TGGCGCGTCC 
ACGAGGGCAT ACACTCTGCG AGAGAAGCCT CAGACATTGC CATCGGCTGT GAAAGGAGAT 
GAGAAGATGG GCGGAGAGGA TGATGAACTC AAGGGCTTGT TGGGACTTCC CGAAGAGGAG 
ACCGAGCTGG ATAACCTGAC AGAGTTCAAC ACTGCCCACA ACAAGCGGAT TTCAACCCTC 
ACTATTGAGG AGGGGAACCT GGACATCCAG AGACCAAAGA GGAAGAGGAA GAACTCAAGG 
GTGACCTTCA GTGAGGATGA CGAGATCATC AACCCAGAGG ACGTGGATCC GTCTGTTGGT 
CGGTTCCGGA ACATGGTGCA GACAGCAGTG GTTCCAGTCA AGAAGCGGAT GGAGGGCTCT 
GGCTCTCTGG GCCTGGAGGA GTCGGGGAGC AGGCGCATGC AGAACTTTGC CTTCAGTGGA 
GGACTATATG GGGGCCTGCC CCCCACACAC AGTGAAACGG GCTCCCAGCC GCATGGCATT 
CATGGGACAG CGCTCATTGG TGGCTTGCCC ATGCCATACC CGAACCTCGC CCCTGATGTG 
GACTTGACTC CTGTTGTGCC ATCAGCAGTG GCCATAAATC CCACACCAAA CCCTGCAGTC 
TATAACCCTG AGGCTGTGAA TGAACCCAAG AAGAAGAAAT ACGCGAAGGA GGCTTGGCCG 
GGCAAGAAGC CCACACCTTC CTTACTGATT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r8 ( NM_001355198.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342127.1 Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu72001
Clone ID Related Accession (Same CDS sequence)	XM_006538464.3 , NM_001355198.1
Accession Version	NM_001355198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	nuclear inhibitor of protein phosphatase 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB855371.1, AL671190.10, BY293277.1, AK032022.1 and BQ749177.1. On Sep 20, 2017 this sequence version replaced XM_006538464.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.207361.1, SRR1660815.12025.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACGAA CGTCTCAGAA AGATCCTGGA ATGATTGAGG GAAAACTGTC CGCTGTGAGG 
GGCCGGGCGA CGTGTGGAGG ATCCTGGAGC TGGGGCCCAG GCTCTGCGGA AACCCGAGTT 
TTGGGGGCTC GAAGGGCAGG TAAACCACCA CCTGGCTTAC ATCTGGATGT GGTGAAAGGA 
GACAAGCTAA TTGAGAAGCT GATTATTGAT GAGAAAAAGT ATTACTTATT TGGGAGGAAC 
CCCGATCTGT GTGACTTCAC TATCGACCAC CAGTCATGCT CTCGAGTCCA CGCAGCGCTG 
GTGTACCACA AACACCTGAA GAGAGTTTTC TTGATAGACC TCAACAGCAC ACATGGCACT 
TTCTTGGGTC ACATTCGGCT GGAACCTCAC AAGCCTCAAC AGATTCCCAT CGATTCTACG 
GTCTCATTTG GCGCGTCCAC GAGGGCATAC ACTCTGCGAG AGAAGCCTCA GACATTGCCA 
TCGGCTGTGA AAGGAGATGA GAAGATGGGC GGAGAGGATG ATGAACTCAA GGGCTTGTTG 
GGACTTCCCG AAGAGGAGAC CGAGCTGGAT AACCTGACAG AGTTCAACAC TGCCCACAAC 
AAGCGGATTT CAACCCTCAC TATTGAGGAG GGGAACCTGG ACATCCAGAG ACCAAAGAGG 
AAGAGGAAGA ACTCAAGGGT GACCTTCAGT GAGGATGACG AGATCATCAA CCCAGAGGAC 
GTGGATCCGT CTGTTGGTCG GTTCCGGAAC ATGGTGCAGA CAGCAGTGGT TCCAGTCAAG 
AAGAAGCGGA TGGAGGGCTC TGGCTCTCTG GGCCTGGAGG AGTCGGGGAG CAGGCGCATG 
CAGAACTTTG CCTTCAGTGG AGGACTATAT GGGGGCCTGC CCCCCACACA CAGTGAAACG 
GGCTCCCAGC CGCATGGCAT TCATGGGACA GCGCTCATTG GTGGCTTGCC CATGCCATAC 
CCGAACCTCG CCCCTGATGT GGACTTGACT CCTGTTGTGC CATCAGCAGT GGCCATAAAT 
CCCACACCAA ACCCTGCAGT CTATAACCCT GAGGCTGTGA ATGAACCCAA GAAGAAGAAA 
TACGCGAAGG AGGCTTGGCC GGGCAAGAAG CCCACACCTT CCTTACTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342127.1
CDS	193..1326
Translation	MKRTSQKDPGMIEGKLSAVRGRATCGGSWSWGPGSAETRVLGARRAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGDEKMGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRMEGSGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSETGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVAINPTPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

Target ORF information:

RefSeq Version	NM_001355198.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355198.1

ORF Insert Sequence:

ATGAAACGAA CGTCTCAGAA AGATCCTGGA ATGATTGAGG GAAAACTGTC CGCTGTGAGG 
GGCCGGGCGA CGTGTGGAGG ATCCTGGAGC TGGGGCCCAG GCTCTGCGGA AACCCGAGTT 
TTGGGGGCTC GAAGGGCAGG TAAACCACCA CCTGGCTTAC ATCTGGATGT GGTGAAAGGA 
GACAAGCTAA TTGAGAAGCT GATTATTGAT GAGAAAAAGT ATTACTTATT TGGGAGGAAC 
CCCGATCTGT GTGACTTCAC TATCGACCAC CAGTCATGCT CTCGAGTCCA CGCAGCGCTG 
GTGTACCACA AACACCTGAA GAGAGTTTTC TTGATAGACC TCAACAGCAC ACATGGCACT 
TTCTTGGGTC ACATTCGGCT GGAACCTCAC AAGCCTCAAC AGATTCCCAT CGATTCTACG 
GTCTCATTTG GCGCGTCCAC GAGGGCATAC ACTCTGCGAG AGAAGCCTCA GACATTGCCA 
TCGGCTGTGA AAGGAGATGA GAAGATGGGC GGAGAGGATG ATGAACTCAA GGGCTTGTTG 
GGACTTCCCG AAGAGGAGAC CGAGCTGGAT AACCTGACAG AGTTCAACAC TGCCCACAAC 
AAGCGGATTT CAACCCTCAC TATTGAGGAG GGGAACCTGG ACATCCAGAG ACCAAAGAGG 
AAGAGGAAGA ACTCAAGGGT GACCTTCAGT GAGGATGACG AGATCATCAA CCCAGAGGAC 
GTGGATCCGT CTGTTGGTCG GTTCCGGAAC ATGGTGCAGA CAGCAGTGGT TCCAGTCAAG 
AAGAAGCGGA TGGAGGGCTC TGGCTCTCTG GGCCTGGAGG AGTCGGGGAG CAGGCGCATG 
CAGAACTTTG CCTTCAGTGG AGGACTATAT GGGGGCCTGC CCCCCACACA CAGTGAAACG 
GGCTCCCAGC CGCATGGCAT TCATGGGACA GCGCTCATTG GTGGCTTGCC CATGCCATAC 
CCGAACCTCG CCCCTGATGT GGACTTGACT CCTGTTGTGC CATCAGCAGT GGCCATAAAT 
CCCACACCAA ACCCTGCAGT CTATAACCCT GAGGCTGTGA ATGAACCCAA GAAGAAGAAA 
TACGCGAAGG AGGCTTGGCC GGGCAAGAAG CCCACACCTT CCTTACTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r8 ( NM_001355199.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001342128.1 Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu72002
Clone ID Related Accession (Same CDS sequence)	XM_006538465.3 , NM_001355199.1
Accession Version	NM_001355199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	nuclear inhibitor of protein phosphatase 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB855371.1, AL671190.10, BY293277.1, AK032022.1 and BQ749177.1. On Sep 20, 2017 this sequence version replaced XM_006538465.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660825.118961.1, SRR1660817.367351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACGAA CGTCTCAGAA AGATCCTGGA ATGATTGAGG GAAAACTGTC CGCTGTGAGG 
GGCCGGGCGA CGTGTGGAGG ATCCTGGAGC TGGGGCCCAG GCTCTGCGGA AACCCGAGTT 
TTGGGGGCTC GAAGGGCAGG TAAACCACCA CCTGGCTTAC ATCTGGATGT GGTGAAAGGA 
GACAAGCTAA TTGAGAAGCT GATTATTGAT GAGAAAAAGT ATTACTTATT TGGGAGGAAC 
CCCGATCTGT GTGACTTCAC TATCGACCAC CAGTCATGCT CTCGAGTCCA CGCAGCGCTG 
GTGTACCACA AACACCTGAA GAGAGTTTTC TTGATAGACC TCAACAGCAC ACATGGCACT 
TTCTTGGGTC ACATTCGGCT GGAACCTCAC AAGCCTCAAC AGATTCCCAT CGATTCTACG 
GTCTCATTTG GCGCGTCCAC GAGGGCATAC ACTCTGCGAG AGAAGCCTCA GACATTGCCA 
TCGGCTGTGA AAGGAGATGA GAAGATGGGC GGAGAGGATG ATGAACTCAA GGGCTTGTTG 
GGACTTCCCG AAGAGGAGAC CGAGCTGGAT AACCTGACAG AGTTCAACAC TGCCCACAAC 
AAGCGGATTT CAACCCTCAC TATTGAGGAG GGGAACCTGG ACATCCAGAG ACCAAAGAGG 
AAGAGGAAGA ACTCAAGGGT GACCTTCAGT GAGGATGACG AGATCATCAA CCCAGAGGAC 
GTGGATCCGT CTGTTGGTCG GTTCCGGAAC ATGGTGCAGA CAGCAGTGGT TCCAGTCAAG 
AAGCGGATGG AGGGCTCTGG CTCTCTGGGC CTGGAGGAGT CGGGGAGCAG GCGCATGCAG 
AACTTTGCCT TCAGTGGAGG ACTATATGGG GGCCTGCCCC CCACACACAG TGAAACGGGC 
TCCCAGCCGC ATGGCATTCA TGGGACAGCG CTCATTGGTG GCTTGCCCAT GCCATACCCG 
AACCTCGCCC CTGATGTGGA CTTGACTCCT GTTGTGCCAT CAGCAGTGGC CATAAATCCC 
ACACCAAACC CTGCAGTCTA TAACCCTGAG GCTGTGAATG AACCCAAGAA GAAGAAATAC 
GCGAAGGAGG CTTGGCCGGG CAAGAAGCCC ACACCTTCCT TACTGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342128.1
CDS	193..1323
Translation	MKRTSQKDPGMIEGKLSAVRGRATCGGSWSWGPGSAETRVLGARRAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGDEKMGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKRMEGSGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSETGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVAINPTPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

Target ORF information:

RefSeq Version	NM_001355199.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355199.1

ORF Insert Sequence:

ATGAAACGAA CGTCTCAGAA AGATCCTGGA ATGATTGAGG GAAAACTGTC CGCTGTGAGG 
GGCCGGGCGA CGTGTGGAGG ATCCTGGAGC TGGGGCCCAG GCTCTGCGGA AACCCGAGTT 
TTGGGGGCTC GAAGGGCAGG TAAACCACCA CCTGGCTTAC ATCTGGATGT GGTGAAAGGA 
GACAAGCTAA TTGAGAAGCT GATTATTGAT GAGAAAAAGT ATTACTTATT TGGGAGGAAC 
CCCGATCTGT GTGACTTCAC TATCGACCAC CAGTCATGCT CTCGAGTCCA CGCAGCGCTG 
GTGTACCACA AACACCTGAA GAGAGTTTTC TTGATAGACC TCAACAGCAC ACATGGCACT 
TTCTTGGGTC ACATTCGGCT GGAACCTCAC AAGCCTCAAC AGATTCCCAT CGATTCTACG 
GTCTCATTTG GCGCGTCCAC GAGGGCATAC ACTCTGCGAG AGAAGCCTCA GACATTGCCA 
TCGGCTGTGA AAGGAGATGA GAAGATGGGC GGAGAGGATG ATGAACTCAA GGGCTTGTTG 
GGACTTCCCG AAGAGGAGAC CGAGCTGGAT AACCTGACAG AGTTCAACAC TGCCCACAAC 
AAGCGGATTT CAACCCTCAC TATTGAGGAG GGGAACCTGG ACATCCAGAG ACCAAAGAGG 
AAGAGGAAGA ACTCAAGGGT GACCTTCAGT GAGGATGACG AGATCATCAA CCCAGAGGAC 
GTGGATCCGT CTGTTGGTCG GTTCCGGAAC ATGGTGCAGA CAGCAGTGGT TCCAGTCAAG 
AAGCGGATGG AGGGCTCTGG CTCTCTGGGC CTGGAGGAGT CGGGGAGCAG GCGCATGCAG 
AACTTTGCCT TCAGTGGAGG ACTATATGGG GGCCTGCCCC CCACACACAG TGAAACGGGC 
TCCCAGCCGC ATGGCATTCA TGGGACAGCG CTCATTGGTG GCTTGCCCAT GCCATACCCG 
AACCTCGCCC CTGATGTGGA CTTGACTCCT GTTGTGCCAT CAGCAGTGGC CATAAATCCC 
ACACCAAACC CTGCAGTCTA TAACCCTGAG GCTGTGAATG AACCCAAGAA GAAGAAATAC 
GCGAAGGAGG CTTGGCCGGG CAAGAAGCCC ACACCTTCCT TACTGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r8 ( XM_006538464.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538527.2 Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 8 (Ppp1r8), transcript variant X1, mRNA.

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CloneID	OMu72001
Clone ID Related Accession (Same CDS sequence)	XM_006538464.3 , NM_001355198.1
Accession Version	XM_006538464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	nuclear inhibitor of protein phosphatase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538464.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAA CGTCTCAGAA AGATCCTGGA ATGATTGAGG GAAAACTGTC CGCTGTGAGG 
GGCCGGGCGA CGTGTGGAGG ATCCTGGAGC TGGGGCCCAG GCTCTGCGGA AACCCGAGTT 
TTGGGGGCTC GAAGGGCAGG TAAACCACCA CCTGGCTTAC ATCTGGATGT GGTGAAAGGA 
GACAAGCTAA TTGAGAAGCT GATTATTGAT GAGAAAAAGT ATTACTTATT TGGGAGGAAC 
CCCGATCTGT GTGACTTCAC TATCGACCAC CAGTCATGCT CTCGAGTCCA CGCAGCGCTG 
GTGTACCACA AACACCTGAA GAGAGTTTTC TTGATAGACC TCAACAGCAC ACATGGCACT 
TTCTTGGGTC ACATTCGGCT GGAACCTCAC AAGCCTCAAC AGATTCCCAT CGATTCTACG 
GTCTCATTTG GCGCGTCCAC GAGGGCATAC ACTCTGCGAG AGAAGCCTCA GACATTGCCA 
TCGGCTGTGA AAGGAGATGA GAAGATGGGC GGAGAGGATG ATGAACTCAA GGGCTTGTTG 
GGACTTCCCG AAGAGGAGAC CGAGCTGGAT AACCTGACAG AGTTCAACAC TGCCCACAAC 
AAGCGGATTT CAACCCTCAC TATTGAGGAG GGGAACCTGG ACATCCAGAG ACCAAAGAGG 
AAGAGGAAGA ACTCAAGGGT GACCTTCAGT GAGGATGACG AGATCATCAA CCCAGAGGAC 
GTGGATCCGT CTGTTGGTCG GTTCCGGAAC ATGGTGCAGA CAGCAGTGGT TCCAGTCAAG 
AAGAAGCGGA TGGAGGGCTC TGGCTCTCTG GGCCTGGAGG AGTCGGGGAG CAGGCGCATG 
CAGAACTTTG CCTTCAGTGG AGGACTATAT GGGGGCCTGC CCCCCACACA CAGTGAAACG 
GGCTCCCAGC CGCATGGCAT TCATGGGACA GCGCTCATTG GTGGCTTGCC CATGCCATAC 
CCGAACCTCG CCCCTGATGT GGACTTGACT CCTGTTGTGC CATCAGCAGT GGCCATAAAT 
CCCACACCAA ACCCTGCAGT CTATAACCCT GAGGCTGTGA ATGAACCCAA GAAGAAGAAA 
TACGCGAAGG AGGCTTGGCC GGGCAAGAAG CCCACACCTT CCTTACTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538527.2
CDS	199..1332
Translation	MKRTSQKDPGMIEGKLSAVRGRATCGGSWSWGPGSAETRVLGARRAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGDEKMGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRMEGSGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSETGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVAINPTPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

Target ORF information:

RefSeq Version	XM_006538464.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 8 (Ppp1r8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538464.3

ORF Insert Sequence:

ATGAAACGAA CGTCTCAGAA AGATCCTGGA ATGATTGAGG GAAAACTGTC CGCTGTGAGG 
GGCCGGGCGA CGTGTGGAGG ATCCTGGAGC TGGGGCCCAG GCTCTGCGGA AACCCGAGTT 
TTGGGGGCTC GAAGGGCAGG TAAACCACCA CCTGGCTTAC ATCTGGATGT GGTGAAAGGA 
GACAAGCTAA TTGAGAAGCT GATTATTGAT GAGAAAAAGT ATTACTTATT TGGGAGGAAC 
CCCGATCTGT GTGACTTCAC TATCGACCAC CAGTCATGCT CTCGAGTCCA CGCAGCGCTG 
GTGTACCACA AACACCTGAA GAGAGTTTTC TTGATAGACC TCAACAGCAC ACATGGCACT 
TTCTTGGGTC ACATTCGGCT GGAACCTCAC AAGCCTCAAC AGATTCCCAT CGATTCTACG 
GTCTCATTTG GCGCGTCCAC GAGGGCATAC ACTCTGCGAG AGAAGCCTCA GACATTGCCA 
TCGGCTGTGA AAGGAGATGA GAAGATGGGC GGAGAGGATG ATGAACTCAA GGGCTTGTTG 
GGACTTCCCG AAGAGGAGAC CGAGCTGGAT AACCTGACAG AGTTCAACAC TGCCCACAAC 
AAGCGGATTT CAACCCTCAC TATTGAGGAG GGGAACCTGG ACATCCAGAG ACCAAAGAGG 
AAGAGGAAGA ACTCAAGGGT GACCTTCAGT GAGGATGACG AGATCATCAA CCCAGAGGAC 
GTGGATCCGT CTGTTGGTCG GTTCCGGAAC ATGGTGCAGA CAGCAGTGGT TCCAGTCAAG 
AAGAAGCGGA TGGAGGGCTC TGGCTCTCTG GGCCTGGAGG AGTCGGGGAG CAGGCGCATG 
CAGAACTTTG CCTTCAGTGG AGGACTATAT GGGGGCCTGC CCCCCACACA CAGTGAAACG 
GGCTCCCAGC CGCATGGCAT TCATGGGACA GCGCTCATTG GTGGCTTGCC CATGCCATAC 
CCGAACCTCG CCCCTGATGT GGACTTGACT CCTGTTGTGC CATCAGCAGT GGCCATAAAT 
CCCACACCAA ACCCTGCAGT CTATAACCCT GAGGCTGTGA ATGAACCCAA GAAGAAGAAA 
TACGCGAAGG AGGCTTGGCC GGGCAAGAAG CCCACACCTT CCTTACTGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r8 ( XM_006538465.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538528.2 Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 8 (Ppp1r8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu72002
Clone ID Related Accession (Same CDS sequence)	XM_006538465.3 , NM_001355199.1
Accession Version	XM_006538465.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	nuclear inhibitor of protein phosphatase 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538465.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACGAA CGTCTCAGAA AGATCCTGGA ATGATTGAGG GAAAACTGTC CGCTGTGAGG 
GGCCGGGCGA CGTGTGGAGG ATCCTGGAGC TGGGGCCCAG GCTCTGCGGA AACCCGAGTT 
TTGGGGGCTC GAAGGGCAGG TAAACCACCA CCTGGCTTAC ATCTGGATGT GGTGAAAGGA 
GACAAGCTAA TTGAGAAGCT GATTATTGAT GAGAAAAAGT ATTACTTATT TGGGAGGAAC 
CCCGATCTGT GTGACTTCAC TATCGACCAC CAGTCATGCT CTCGAGTCCA CGCAGCGCTG 
GTGTACCACA AACACCTGAA GAGAGTTTTC TTGATAGACC TCAACAGCAC ACATGGCACT 
TTCTTGGGTC ACATTCGGCT GGAACCTCAC AAGCCTCAAC AGATTCCCAT CGATTCTACG 
GTCTCATTTG GCGCGTCCAC GAGGGCATAC ACTCTGCGAG AGAAGCCTCA GACATTGCCA 
TCGGCTGTGA AAGGAGATGA GAAGATGGGC GGAGAGGATG ATGAACTCAA GGGCTTGTTG 
GGACTTCCCG AAGAGGAGAC CGAGCTGGAT AACCTGACAG AGTTCAACAC TGCCCACAAC 
AAGCGGATTT CAACCCTCAC TATTGAGGAG GGGAACCTGG ACATCCAGAG ACCAAAGAGG 
AAGAGGAAGA ACTCAAGGGT GACCTTCAGT GAGGATGACG AGATCATCAA CCCAGAGGAC 
GTGGATCCGT CTGTTGGTCG GTTCCGGAAC ATGGTGCAGA CAGCAGTGGT TCCAGTCAAG 
AAGCGGATGG AGGGCTCTGG CTCTCTGGGC CTGGAGGAGT CGGGGAGCAG GCGCATGCAG 
AACTTTGCCT TCAGTGGAGG ACTATATGGG GGCCTGCCCC CCACACACAG TGAAACGGGC 
TCCCAGCCGC ATGGCATTCA TGGGACAGCG CTCATTGGTG GCTTGCCCAT GCCATACCCG 
AACCTCGCCC CTGATGTGGA CTTGACTCCT GTTGTGCCAT CAGCAGTGGC CATAAATCCC 
ACACCAAACC CTGCAGTCTA TAACCCTGAG GCTGTGAATG AACCCAAGAA GAAGAAATAC 
GCGAAGGAGG CTTGGCCGGG CAAGAAGCCC ACACCTTCCT TACTGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538528.2
CDS	201..1331
Translation	MKRTSQKDPGMIEGKLSAVRGRATCGGSWSWGPGSAETRVLGARRAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGDEKMGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKRMEGSGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSETGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVAINPTPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

Target ORF information:

RefSeq Version	XM_006538465.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 8 (Ppp1r8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538465.3

ORF Insert Sequence:

ATGAAACGAA CGTCTCAGAA AGATCCTGGA ATGATTGAGG GAAAACTGTC CGCTGTGAGG 
GGCCGGGCGA CGTGTGGAGG ATCCTGGAGC TGGGGCCCAG GCTCTGCGGA AACCCGAGTT 
TTGGGGGCTC GAAGGGCAGG TAAACCACCA CCTGGCTTAC ATCTGGATGT GGTGAAAGGA 
GACAAGCTAA TTGAGAAGCT GATTATTGAT GAGAAAAAGT ATTACTTATT TGGGAGGAAC 
CCCGATCTGT GTGACTTCAC TATCGACCAC CAGTCATGCT CTCGAGTCCA CGCAGCGCTG 
GTGTACCACA AACACCTGAA GAGAGTTTTC TTGATAGACC TCAACAGCAC ACATGGCACT 
TTCTTGGGTC ACATTCGGCT GGAACCTCAC AAGCCTCAAC AGATTCCCAT CGATTCTACG 
GTCTCATTTG GCGCGTCCAC GAGGGCATAC ACTCTGCGAG AGAAGCCTCA GACATTGCCA 
TCGGCTGTGA AAGGAGATGA GAAGATGGGC GGAGAGGATG ATGAACTCAA GGGCTTGTTG 
GGACTTCCCG AAGAGGAGAC CGAGCTGGAT AACCTGACAG AGTTCAACAC TGCCCACAAC 
AAGCGGATTT CAACCCTCAC TATTGAGGAG GGGAACCTGG ACATCCAGAG ACCAAAGAGG 
AAGAGGAAGA ACTCAAGGGT GACCTTCAGT GAGGATGACG AGATCATCAA CCCAGAGGAC 
GTGGATCCGT CTGTTGGTCG GTTCCGGAAC ATGGTGCAGA CAGCAGTGGT TCCAGTCAAG 
AAGCGGATGG AGGGCTCTGG CTCTCTGGGC CTGGAGGAGT CGGGGAGCAG GCGCATGCAG 
AACTTTGCCT TCAGTGGAGG ACTATATGGG GGCCTGCCCC CCACACACAG TGAAACGGGC 
TCCCAGCCGC ATGGCATTCA TGGGACAGCG CTCATTGGTG GCTTGCCCAT GCCATACCCG 
AACCTCGCCC CTGATGTGGA CTTGACTCCT GTTGTGCCAT CAGCAGTGGC CATAAATCCC 
ACACCAAACC CTGCAGTCTA TAACCCTGAG GCTGTGAATG AACCCAAGAA GAAGAAATAC 
GCGAAGGAGG CTTGGCCGGG CAAGAAGCCC ACACCTTCCT TACTGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r8 ( NM_146154.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666266.1 Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14284
Clone ID Related Accession (Same CDS sequence)	NM_146154.3
Accession Version	NM_146154.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	nuclear inhibitor of protein phosphatase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY293277.1, AK032022.1 and BQ749177.1. On Mar 19, 2014 this sequence version replaced NM_146154.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC025479.1, SRR1660817.121887.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CCGTGAACTC CGGCTCCAGC CTCCCGCTGT TCGACTGCCC GACTTGGGCA 
GGTAAACCAC CACCTGGCTT ACATCTGGAT GTGGTGAAAG GAGACAAGCT AATTGAGAAG 
CTGATTATTG ATGAGAAAAA GTATTACTTA TTTGGGAGGA ACCCCGATCT GTGTGACTTC 
ACTATCGACC ACCAGTCATG CTCTCGAGTC CACGCAGCGC TGGTGTACCA CAAACACCTG 
AAGAGAGTTT TCTTGATAGA CCTCAACAGC ACACATGGCA CTTTCTTGGG TCACATTCGG 
CTGGAACCTC ACAAGCCTCA ACAGATTCCC ATCGATTCTA CGGTCTCATT TGGCGCGTCC 
ACGAGGGCAT ACACTCTGCG AGAGAAGCCT CAGACATTGC CATCGGCTGT GAAAGGAGAT 
GAGAAGATGG GCGGAGAGGA TGATGAACTC AAGGGCTTGT TGGGACTTCC CGAAGAGGAG 
ACCGAGCTGG ATAACCTGAC AGAGTTCAAC ACTGCCCACA ACAAGCGGAT TTCAACCCTC 
ACTATTGAGG AGGGGAACCT GGACATCCAG AGACCAAAGA GGAAGAGGAA GAACTCAAGG 
GTGACCTTCA GTGAGGATGA CGAGATCATC AACCCAGAGG ACGTGGATCC GTCTGTTGGT 
CGGTTCCGGA ACATGGTGCA GACAGCAGTG GTTCCAGTCA AGAAGAAGCG GATGGAGGGC 
TCTGGCTCTC TGGGCCTGGA GGAGTCGGGG AGCAGGCGCA TGCAGAACTT TGCCTTCAGT 
GGAGGACTAT ATGGGGGCCT GCCCCCCACA CACAGTGAAA CGGGCTCCCA GCCGCATGGC 
ATTCATGGGA CAGCGCTCAT TGGTGGCTTG CCCATGCCAT ACCCGAACCT CGCCCCTGAT 
GTGGACTTGA CTCCTGTTGT GCCATCAGCA GTGGCCATAA ATCCCACACC AAACCCTGCA 
GTCTATAACC CTGAGGCTGT GAATGAACCC AAGAAGAAGA AATACGCGAA GGAGGCTTGG 
CCGGGCAAGA AGCCCACACC TTCCTTACTG ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666266.1
CDS	58..1113
Translation	MAAAVNSGSSLPLFDCPTWAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGDEKMGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRMEGSGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSETGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVAINPTPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

Target ORF information:

RefSeq Version	NM_146154.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory subunit 8 (Ppp1r8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146154.3

ORF Insert Sequence:

ATGGCGGCAG CCGTGAACTC CGGCTCCAGC CTCCCGCTGT TCGACTGCCC GACTTGGGCA 
GGTAAACCAC CACCTGGCTT ACATCTGGAT GTGGTGAAAG GAGACAAGCT AATTGAGAAG 
CTGATTATTG ATGAGAAAAA GTATTACTTA TTTGGGAGGA ACCCCGATCT GTGTGACTTC 
ACTATCGACC ACCAGTCATG CTCTCGAGTC CACGCAGCGC TGGTGTACCA CAAACACCTG 
AAGAGAGTTT TCTTGATAGA CCTCAACAGC ACACATGGCA CTTTCTTGGG TCACATTCGG 
CTGGAACCTC ACAAGCCTCA ACAGATTCCC ATCGATTCTA CGGTCTCATT TGGCGCGTCC 
ACGAGGGCAT ACACTCTGCG AGAGAAGCCT CAGACATTGC CATCGGCTGT GAAAGGAGAT 
GAGAAGATGG GCGGAGAGGA TGATGAACTC AAGGGCTTGT TGGGACTTCC CGAAGAGGAG 
ACCGAGCTGG ATAACCTGAC AGAGTTCAAC ACTGCCCACA ACAAGCGGAT TTCAACCCTC 
ACTATTGAGG AGGGGAACCT GGACATCCAG AGACCAAAGA GGAAGAGGAA GAACTCAAGG 
GTGACCTTCA GTGAGGATGA CGAGATCATC AACCCAGAGG ACGTGGATCC GTCTGTTGGT 
CGGTTCCGGA ACATGGTGCA GACAGCAGTG GTTCCAGTCA AGAAGAAGCG GATGGAGGGC 
TCTGGCTCTC TGGGCCTGGA GGAGTCGGGG AGCAGGCGCA TGCAGAACTT TGCCTTCAGT 
GGAGGACTAT ATGGGGGCCT GCCCCCCACA CACAGTGAAA CGGGCTCCCA GCCGCATGGC 
ATTCATGGGA CAGCGCTCAT TGGTGGCTTG CCCATGCCAT ACCCGAACCT CGCCCCTGAT 
GTGGACTTGA CTCCTGTTGT GCCATCAGCA GTGGCCATAA ATCCCACACC AAACCCTGCA 
GTCTATAACC CTGAGGCTGT GAATGAACCC AAGAAGAAGA AATACGCGAA GGAGGCTTGG 
CCGGGCAAGA AGCCCACACC TTCCTTACTG ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The protein phosphatase 1 regulator NIPP1 is essential for mammalian spermatogenesis.
Scientific reports7(1)13364(2017 Oct)
Ferreira M,Boens S,Winkler C,Szek?r K,Verbinnen I,Van Eynde A,Fardilha M,Bollen M

Brief Report: The Deletion of the Phosphatase Regulator NIPP1 Causes Progenitor Cell Expansion in the Adult Liver.
Stem cells (Dayton, Ohio)34(8)2256-62(2016 08)
Boens S,Verbinnen I,Verhulst S,Szek?r K,Ferreira M,Gevaert T,Baes M,Roskams T,van Grunsven LA,Van Eynde A,Bollen M

BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes.
Nature structural & molecular biology23(5)387-94(2016 05)
Yu C,Ji SY,Sha QQ,Dang Y,Zhou JJ,Zhang YL,Liu Y,Wang ZW,Hu B,Sun QY,Sun SC,Tang F,Fan HY

An AUTS2-Polycomb complex activates gene expression in the CNS.
Nature516(7531)349-54(2014 Dec)
Gao Z,Lee P,Stafford JM,von Schimmelmann M,Schaefer A,Reinberg D

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Ppp1r8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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