COL27A1 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL27A1
Entrez Gene ID	708556
Full Name	collagen type XXVII alpha 1 chain
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_028834926.1	XP_028690759.1	collagen alpha-1(XXVII) chain isoform X3
XM_015117009.2	XP_014972495.2	collagen alpha-1(XXVII) chain isoform X1
XM_015117010.2	XP_014972496.2	collagen alpha-1(XXVII) chain isoform X4
XM_015117010.1	XP_014972496.1	collagen alpha-1(XXVII) chain isoform X2
XM_028834925.1	XP_028690758.1	collagen alpha-1(XXVII) chain isoform X2
XM_028834927.1	XP_028690760.1	collagen alpha-1(XXVII) chain isoform X5
XM_015117009.1	XP_014972495.1	collagen alpha-1(XXVII) chain isoform X1
XM_028834928.1	XP_028690761.1	collagen alpha-1(XXVII) chain isoform X6

Pathways from BioSystems
Gene Ontology

The following COL27A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL27A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMb71338	XM_015117009.2 Latest version!	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb71339	XM_028834925.1 Latest version!	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb71340	XM_028834926.1 Latest version!	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb71341	XM_015117010.2 Latest version!	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb71342	XM_028834927.1 Latest version!	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMb71343	XM_028834928.1 Latest version!	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMb48770	XM_015117009.1 Latest version!	Macaca mulatta collagen, type XXVII, alpha 1 (COL27A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OMb48771	XM_015117010.1 Latest version!	Macaca mulatta collagen, type XXVII, alpha 1 (COL27A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL27A1 ( XM_015117009.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014972495.2 Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMb71338
Clone ID Related Accession (Same CDS sequence)	XM_015117009.2
Accession Version	XM_015117009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015117009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGGGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGTCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAAACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGACA GAAAGGGGAC CCAGGGCTCT CACCAGGAAA GGCCCACGAT 
GGGGCAAAGG GCGACATGGG CTTGCCTGGG CTGTCCGGGA ATCCAGGACC TCCGGGACGA 
AAGGGACACA AGGGCTATCC TGGACCGGCA GGGCACCCCG GAGAACAGGG GCAGCCAGGA 
CCTGAGGGCA GCCCAGGGGC CAAAGGTTAC CCTGGCAGGC AGGGGTTACC TGGACCGGTA 
GGAGATCCCG GCCCCAAAGG CAGCAGGGGC TACATTGGGC TCCCAGGGCT CTTCGGCCTG 
CCAGGGTCTG ATGGAGAGCG AGGCCTTCCT GGTGTTCCTG GCAAGAGGGG CAAGATGGGT 
ATGCCGGGGT TTCCTGGAGT CTTTGGGGAA AGAGGCCCTC CCGGACTGGA TGGAAACCCT 
GGAGAACTGG GCCTGCCAGG CCCCCCTGGA GTCCCCGGCC TCATTGGTGA CTTGGGAGTG 
TTGGGTCCGA TTGGCTACCC AGGACCCAAG GGCATGAAGG GACTGATGGG CAGCGTGGGG 
GAGCCCGGAC TGAAAGGTGA TAAGGGTGAA CAAGGGGTTC CAGGTGTGTC GGGAGATCCC 
GGATTCCAAG GAGACAAGGG GAGCCAGGGG TTGCCAGGGT TCCCCGGCGC ACGGGGGAAG 
CCAGGGCCTC TGGGCAAAGT TGGAGACAAA GGATCCATTG GGTTTCCCGG GCCCCCTGGA 
CCCGAGGGAT TCCCAGGAGA CATCGGCCCC CCTGGTGACA ACGGCCCAGA AGGCATGAAG 
GGTAAGCCTG GAGCCCGAGG CCTGCCGGGA CCCCGTGGGC AGCTGGGGCC CGAGGGAGAT 
GAGGGACCCA TGGGGCCACC AGGGGCCCCT GGCTTGGAGG GTCAGCCTGG CAGGAAGGGC 
TTTCCTGGGA GGCCCGGCCT GGATGGCGTG AAGGGGGAAC CGGGAGATCC TGGTCGGCCA 
GGGCCTGTGG GAGAGCAGGG ATTTATGGGA TTCATTGGTC TGGTCGGGGA GCCAGGAATC 
GTGGGAGAAA AGGGTGATCG TGGCATGATG GGACCCCCAG GCGTGCCTGG ACCCAAGGGG 
TCGATGGGTC ATCCTGGAAT GCCAGGTGGT ATGGGGACCC CTGGAGAACC TGGACCCCAG 
GGTCCTCCAG GATCTCGAGG CCCACCAGGC ATGAGGGGAG CGAAGGGACG TCGGGGCCCC 
CGAGGACCGG ACGGACCAGC TGGGGAGCAA GGGTCCAGGG GCCTGAAGGG CCCTCCAGGA 
CCCCAGGGCA GACCGGGCCG GCCTGGACAG CAGGGTGTGG CTGGTGAGCA AGGCCACTTG 
GGCTCGCGAG GCTTTCCCGG CATCCCGGGT CCCTCAGGCC CCCCAGGCAC CAAGGGCCTC 
CCAGGAGAAC CGGGCCCTCA GGGACCCCAG GGGCCAGTTG GGCCTCCAGG AGAGATGGGA 
CCTAAGGGGC CGCCGGGTGC AGCGGGAGAA CCGGGCCTTC CTGGGGAAGC TGGGATGAAG 
GGTGACCTTG GACCCCTGGG CACCCCTGGG GAGCAGGGCC TCATTGGGCA ACGGGGAGAG 
CCAGGCCTCG AGGGTGACAG TGGCCCCATG GGACCTGATG GGCTGAAGGG GGACAGGGGA 
GACCCAGGGC CTGATGGAGA ACATGGCGAG AAAGGCCAGG AAGGGCTGAT GGGTGAGGAC 
GGGCCCCCCG GCCCCCCTGG TGTCACTGGT GTCCGGGGTC CTGAAGGAAA ATCAGGGAAG 
CAAGGTGAGA AGGGCCGCAC TGGAGCCAAG GGCGCCAAGG GCTACCAAGG ACAGCTGGGT 
GAGATGGGCG TCCCTGGAGA CCCCGGACCC CCTGGCACTC CAGGCCCTAA AGGGTCCCGG 
GGCAGCCTGG GACCAACGGG TGCTCCTGGA CGCATGGGGG CCCAAGGAGA ACTGGGACTG 
GCTGGTTATG ATGGACACAA AGGCATTGTG GGACCCCTCG GACCTCCTGG ACCAAAAGGC 
GAAAAGGGGG AGCAGGGCGA GGACGGCAAG GCCGAGGGGC CCCCTGGGCC ACCTGGAGAT 
CGGGGCCCTG TGGGTGATCG AGGAGACCGC GGGGAACCCG GGGACCCTGG GTACCCTGGA 
CAGGAGGGTG TGCAAGGCCT CCGTGGAAAG CCAGGCCAGC AGGGCCAACC CGGGCATCCG 
GGACCCCGGG GGCGGCCGGG ACCCAAAGGA TCTAAAGGCG CAGAGGGACC AAAGGGAAAG 
CAAGGCAAGG CGGGGGCCCC AGGCCGGAGG GGGGTCCAGG GCCTGCAGGG GCTGCCAGGG 
CCCCGGGGCG TGGTGGGGAG ACAGGGCCTC GAGGGCATCG CTGGAGCAGA TGGGCTTCCT 
GGCAGGGACG GGCAAGTGGG ACAGCAGGGG GAGCAGGGAG ACGATGGGGA CCCTGGCCCC 
ATGGGCCCTG CTGGGAAGAG AGGAAATCCA GGTGTGGCCG GCTTACCTGG AGCACAGGGA 
CCCCCAGGAT TCAAGGGTGA GAGTGGGTTA CCCGGACAGC TGGGTCCCCC TGGCAAACGA 
GGGACGGAGG GCAGAACAGG GCTCCCTGGA AACCAGGGGG AGCCTGGGTC CAAAGGCCAG 
CCGGGCGACT CTGGTGAGAT GGGCTTCCCA GGAATGGCAG GTCTCTTCGG ACCTAAGGGC 
CCCCCTGGAG ACATCGGCTT CAAAGGCATC CAGGGTCCTC GGGGGCCACC CGGTTTGATG 
GGAAAGGAAG GCATCATCGG GCCCCTCGGA ATCCTGGGAC CTTCGGGACT CCCGGGTCCG 
AAGGGTGACA AAGGCAGCCG TGGGGACTGG GGATTGCAAG GTCCGAGGGG TCCTCCTGGC 
CCCAGAGGGC GGCCCGGCCC CCCGGGTCCT CCAGGGGGTC CTATCCAATT GCAACAAGAT 
GATCTTGGGG TAGCTTTCCA GACGTGGATG GACACCAGTG GAGCACTCAG ACCAGAGGGT 
TACAGCTATC CGGACCGGCT GGTGCTTGAC CAGGGAGGAG AGATCTTTAA AACCTTACAC 
TACCTCAGCA ACCTCATCCA GAGCATTAAG ACGCCCCTGG GCACCAAAGA GAACCCCGCC 
CGGGTCTGCA GGGACCTCAT GGACTGTGAG CAGAAGATGG TGGATGGTAC CTACTGGGTG 
GATCCAAACC TTGGCTGCTC ATCCGACACC ATTGAAGTCT CCTGCAACTT CACGCATGGT 
GGACAGACGT GTCTCAAACC CATCACGGCC TCCAAGGTCG AGTTTGCCAT CAGCCGGGTC 
CAGATGAATT TCCTGCACCT GCTAAGCTCT GAGGTGACCC AGCATATCAC CATCCACTGC 
CTTAACATGA CTGTGTGGCA GGAGGGCACT GGGCAGACCC CAGCCAAGCA GGCCGTGCGC 
TTCCGGGCCT GGAACGGACA GATTTTTGAA GCTGGGGGTC AGTTCCGGCC CGAGGTGTCC 
ATGGATGGCT GCAAGGTCCA AGACGGCCGC TGGCATCAGA CACTCTTCAC CTTCCGGACC 
CAAGACCCCC AACAGCTGCC CATCGTCAGT GTGGACAACC TCCCTCCTGC CTCATCAGGG 
AAGCAGTACC GCCTGGAAGT TGGACCTGCG TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014972495.2
CDS	1..5562
Translation	MGAGSARGARGTATAAAAHGGGFLFSWILVSFACHLASTQGAPEDVDVLQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGSVIPASLGTELALVLSLCSHRVNHAFLFAVRSQKHKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPLGSQPAGRGPKGTVVPATPTKPQRTSPTNPHQHIAGRGPAQTPPAKLSASNTLDPVLPASLGSSTRTPRPAASQPSQKITATKIPKSLPTKPSAPSTSIVPVKSPHPTQKTAAPSFTKSAPPTQKHVPTVPAKVSRPAEKPIQRNQGMPRPPLPSTQPLPPTTSSSKKPIPTLAQTEAKVTNHASKPASARTSTHKPPPFTALSSSPAPTPGSTRTTRPPATMVPPTSGTSAPRTAPAVPTPGSAPTGNKKPTGSEASKKAGPESSPRKPVPLRPGKAARDVPLSDLTTRPSPRQSQPSQQTTSALVLAPAQFLSSSPRPTSSGYSFFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGEQGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPVGPPGEMGPKGPPGAAGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGELGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGRPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGADGLPGRDGQVGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGVAFQTWMDTSGALRPEGYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIVSVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_015117009.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015117009.2

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGGGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGTCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAAACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGACA GAAAGGGGAC CCAGGGCTCT CACCAGGAAA GGCCCACGAT 
GGGGCAAAGG GCGACATGGG CTTGCCTGGG CTGTCCGGGA ATCCAGGACC TCCGGGACGA 
AAGGGACACA AGGGCTATCC TGGACCGGCA GGGCACCCCG GAGAACAGGG GCAGCCAGGA 
CCTGAGGGCA GCCCAGGGGC CAAAGGTTAC CCTGGCAGGC AGGGGTTACC TGGACCGGTA 
GGAGATCCCG GCCCCAAAGG CAGCAGGGGC TACATTGGGC TCCCAGGGCT CTTCGGCCTG 
CCAGGGTCTG ATGGAGAGCG AGGCCTTCCT GGTGTTCCTG GCAAGAGGGG CAAGATGGGT 
ATGCCGGGGT TTCCTGGAGT CTTTGGGGAA AGAGGCCCTC CCGGACTGGA TGGAAACCCT 
GGAGAACTGG GCCTGCCAGG CCCCCCTGGA GTCCCCGGCC TCATTGGTGA CTTGGGAGTG 
TTGGGTCCGA TTGGCTACCC AGGACCCAAG GGCATGAAGG GACTGATGGG CAGCGTGGGG 
GAGCCCGGAC TGAAAGGTGA TAAGGGTGAA CAAGGGGTTC CAGGTGTGTC GGGAGATCCC 
GGATTCCAAG GAGACAAGGG GAGCCAGGGG TTGCCAGGGT TCCCCGGCGC ACGGGGGAAG 
CCAGGGCCTC TGGGCAAAGT TGGAGACAAA GGATCCATTG GGTTTCCCGG GCCCCCTGGA 
CCCGAGGGAT TCCCAGGAGA CATCGGCCCC CCTGGTGACA ACGGCCCAGA AGGCATGAAG 
GGTAAGCCTG GAGCCCGAGG CCTGCCGGGA CCCCGTGGGC AGCTGGGGCC CGAGGGAGAT 
GAGGGACCCA TGGGGCCACC AGGGGCCCCT GGCTTGGAGG GTCAGCCTGG CAGGAAGGGC 
TTTCCTGGGA GGCCCGGCCT GGATGGCGTG AAGGGGGAAC CGGGAGATCC TGGTCGGCCA 
GGGCCTGTGG GAGAGCAGGG ATTTATGGGA TTCATTGGTC TGGTCGGGGA GCCAGGAATC 
GTGGGAGAAA AGGGTGATCG TGGCATGATG GGACCCCCAG GCGTGCCTGG ACCCAAGGGG 
TCGATGGGTC ATCCTGGAAT GCCAGGTGGT ATGGGGACCC CTGGAGAACC TGGACCCCAG 
GGTCCTCCAG GATCTCGAGG CCCACCAGGC ATGAGGGGAG CGAAGGGACG TCGGGGCCCC 
CGAGGACCGG ACGGACCAGC TGGGGAGCAA GGGTCCAGGG GCCTGAAGGG CCCTCCAGGA 
CCCCAGGGCA GACCGGGCCG GCCTGGACAG CAGGGTGTGG CTGGTGAGCA AGGCCACTTG 
GGCTCGCGAG GCTTTCCCGG CATCCCGGGT CCCTCAGGCC CCCCAGGCAC CAAGGGCCTC 
CCAGGAGAAC CGGGCCCTCA GGGACCCCAG GGGCCAGTTG GGCCTCCAGG AGAGATGGGA 
CCTAAGGGGC CGCCGGGTGC AGCGGGAGAA CCGGGCCTTC CTGGGGAAGC TGGGATGAAG 
GGTGACCTTG GACCCCTGGG CACCCCTGGG GAGCAGGGCC TCATTGGGCA ACGGGGAGAG 
CCAGGCCTCG AGGGTGACAG TGGCCCCATG GGACCTGATG GGCTGAAGGG GGACAGGGGA 
GACCCAGGGC CTGATGGAGA ACATGGCGAG AAAGGCCAGG AAGGGCTGAT GGGTGAGGAC 
GGGCCCCCCG GCCCCCCTGG TGTCACTGGT GTCCGGGGTC CTGAAGGAAA ATCAGGGAAG 
CAAGGTGAGA AGGGCCGCAC TGGAGCCAAG GGCGCCAAGG GCTACCAAGG ACAGCTGGGT 
GAGATGGGCG TCCCTGGAGA CCCCGGACCC CCTGGCACTC CAGGCCCTAA AGGGTCCCGG 
GGCAGCCTGG GACCAACGGG TGCTCCTGGA CGCATGGGGG CCCAAGGAGA ACTGGGACTG 
GCTGGTTATG ATGGACACAA AGGCATTGTG GGACCCCTCG GACCTCCTGG ACCAAAAGGC 
GAAAAGGGGG AGCAGGGCGA GGACGGCAAG GCCGAGGGGC CCCCTGGGCC ACCTGGAGAT 
CGGGGCCCTG TGGGTGATCG AGGAGACCGC GGGGAACCCG GGGACCCTGG GTACCCTGGA 
CAGGAGGGTG TGCAAGGCCT CCGTGGAAAG CCAGGCCAGC AGGGCCAACC CGGGCATCCG 
GGACCCCGGG GGCGGCCGGG ACCCAAAGGA TCTAAAGGCG CAGAGGGACC AAAGGGAAAG 
CAAGGCAAGG CGGGGGCCCC AGGCCGGAGG GGGGTCCAGG GCCTGCAGGG GCTGCCAGGG 
CCCCGGGGCG TGGTGGGGAG ACAGGGCCTC GAGGGCATCG CTGGAGCAGA TGGGCTTCCT 
GGCAGGGACG GGCAAGTGGG ACAGCAGGGG GAGCAGGGAG ACGATGGGGA CCCTGGCCCC 
ATGGGCCCTG CTGGGAAGAG AGGAAATCCA GGTGTGGCCG GCTTACCTGG AGCACAGGGA 
CCCCCAGGAT TCAAGGGTGA GAGTGGGTTA CCCGGACAGC TGGGTCCCCC TGGCAAACGA 
GGGACGGAGG GCAGAACAGG GCTCCCTGGA AACCAGGGGG AGCCTGGGTC CAAAGGCCAG 
CCGGGCGACT CTGGTGAGAT GGGCTTCCCA GGAATGGCAG GTCTCTTCGG ACCTAAGGGC 
CCCCCTGGAG ACATCGGCTT CAAAGGCATC CAGGGTCCTC GGGGGCCACC CGGTTTGATG 
GGAAAGGAAG GCATCATCGG GCCCCTCGGA ATCCTGGGAC CTTCGGGACT CCCGGGTCCG 
AAGGGTGACA AAGGCAGCCG TGGGGACTGG GGATTGCAAG GTCCGAGGGG TCCTCCTGGC 
CCCAGAGGGC GGCCCGGCCC CCCGGGTCCT CCAGGGGGTC CTATCCAATT GCAACAAGAT 
GATCTTGGGG TAGCTTTCCA GACGTGGATG GACACCAGTG GAGCACTCAG ACCAGAGGGT 
TACAGCTATC CGGACCGGCT GGTGCTTGAC CAGGGAGGAG AGATCTTTAA AACCTTACAC 
TACCTCAGCA ACCTCATCCA GAGCATTAAG ACGCCCCTGG GCACCAAAGA GAACCCCGCC 
CGGGTCTGCA GGGACCTCAT GGACTGTGAG CAGAAGATGG TGGATGGTAC CTACTGGGTG 
GATCCAAACC TTGGCTGCTC ATCCGACACC ATTGAAGTCT CCTGCAACTT CACGCATGGT 
GGACAGACGT GTCTCAAACC CATCACGGCC TCCAAGGTCG AGTTTGCCAT CAGCCGGGTC 
CAGATGAATT TCCTGCACCT GCTAAGCTCT GAGGTGACCC AGCATATCAC CATCCACTGC 
CTTAACATGA CTGTGTGGCA GGAGGGCACT GGGCAGACCC CAGCCAAGCA GGCCGTGCGC 
TTCCGGGCCT GGAACGGACA GATTTTTGAA GCTGGGGGTC AGTTCCGGCC CGAGGTGTCC 
ATGGATGGCT GCAAGGTCCA AGACGGCCGC TGGCATCAGA CACTCTTCAC CTTCCGGACC 
CAAGACCCCC AACAGCTGCC CATCGTCAGT GTGGACAACC TCCCTCCTGC CTCATCAGGG 
AAGCAGTACC GCCTGGAAGT TGGACCTGCG TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_028834925.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028690758.1 Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

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CloneID	OMb71339
Clone ID Related Accession (Same CDS sequence)	XM_028834925.1
Accession Version	XM_028834925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTAG GCGCCGGAGG AAGGACCGAC CGGCCAGGCT CGCACTTAGG AACCCAGGGG 
TTTCTCTTCT CCTGGATCTT AGTCTCGTTT GCCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGACGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGAGCCCT 
GCACCCCCGG GAGTCATTCC TTTCCAGTCT GGCTTCATCT TTACACAGCG GGCCCGGCTC 
CAGGCTCCCA CGGGCTCCGT CATTCCTGCC TCCCTGGGCA CAGAGCTGGC GCTGGTGCTG 
AGCCTCTGCT CCCACCGGGT GAACCATGCC TTCCTCTTCG CTGTCCGCAG CCAGAAACAC 
AAGCTGCAGC TGGGCCTGCA GTTCCTCCCC GGCAAGACGG TCGTCCACCT GGGGTCCCGG 
CGTTCGGTGG CCTTCGACCT CGACATGCAC GACGGGCGCT GGCACCACCT GGCCCTCGAG 
CTCCGAGGCC GCACAGTCAC TCTGGTGACC GCCTGCGGGC AGCGCCGGGT GCCTGTCCTG 
CTGCCTTTCC ACAGGGACCC TGCACTCGAC CCCGGGGGCT CCTTCCTCTT TGGGAAGATG 
AACCCGCATG CAGTCCAGTT TGAAGGTGCT CTGTGCCAGT TCAGTATCTA CCCTGTGACG 
CAGGTCGCTC ACAATTACTG TACCCACCTG AGGAAGCAGT GTGGACAGGC TGACACGTAC 
CAGTCCCCAC TGGGGCCTCT CTTCTCCCAA GACTCTGGCA GACCTTTCAC CTTCCAGTCT 
GACCTCGCCC TGCTAGGCCT GGAGAACTTG ACCACTGCCA CACCACTGGG GTCACAGCCA 
GCAGGCAGGG GACCCAAGGG GACTGTGGTG CCCGCCACTC CCACCAAGCC CCAAAGGACT 
AGCCCCACAA ACCCTCACCA GCATATCGCG GGGCGAGGCC CAGCCCAAAC CCCACCTGCC 
AAGCTGTCAG CCAGTAACAC ACTTGATCCT GTGCTCCCAG CCTCTCTTGG CAGCTCTACC 
AGAACGCCTC GCCCTGCAGC CTCTCAACCA TCACAGAAGA TCACAGCCAC CAAAATCCCC 
AAAAGCCTCC CTACCAAGCC TTCAGCCCCT TCTACTTCAA TTGTCCCCGT CAAAAGTCCC 
CATCCTACCC AGAAAACAGC TGCACCTTCA TTTACAAAGT CAGCCCCACC CACTCAGAAG 
CATGTACCTA CAGTTCCTGC CAAAGTATCC CGTCCCGCAG AGAAGCCCAT CCAGAGGAAC 
CAAGGAATGC CCAGGCCCCC ACTGCCCAGC ACCCAGCCCC TACCTCCTAC CACCAGCTCC 
TCTAAAAAAC CCATTCCCAC ACTAGCTCAA ACTGAGGCCA AGGTGACTAA CCATGCCAGT 
AAGCCGGCCT CTGCCCGCAC CAGCACCCAC AAACCTCCCC CATTTACTGC TTTATCCTCA 
TCTCCTGCCC CTACTCCTGG TTCTACCAGG ACGACTCGGC CACCAGCCAC AATGGTGCCT 
CCAACCTCGG GCACCAGCGC TCCCAGAACA GCACCTGCCG TCCCCACTCC TGGCTCAGCT 
CCCACTGGAA ACAAGAAGCC CACTGGATCG GAAGCCTCAA AGAAAGCCGG ACCTGAGAGC 
AGCCCCCGGA AGCCCGTCCC CCTCAGACCT GGGAAGGCAG CCAGGGATGT CCCCTTGAGC 
GATCTGACAA CCAGGCCTAG CCCCAGACAG TCCCAGCCCA GTCAGCAGAC CACTTCAGCC 
CTGGTATTGG CCCCAGCTCA ATTCCTGTCC TCCAGCCCCC GGCCCACGAG CAGTGGCTAT 
TCGTTCTTCC ACCTGGCAGG ATCTACGCCT TTCCCTCTGC TGATGGGGCC TCCGGGGCCC 
AAGGGAGACT GTGGTTTGCC GGGTCCCCCT GGGCTGCCTG GGCTACCTGG AATCCCTGGT 
GCACGTGGGC CTCGGGGTCC TCCTGGGCCT TATGGAAATC CAGGTCTCCC CGGCCCTCCT 
GGAGCCAAAG GACAGAAAGG GGACCCAGGG CTCTCACCAG GAAAGGCCCA CGATGGGGCA 
AAGGGCGACA TGGGCTTGCC TGGGCTGTCC GGGAATCCAG GACCTCCGGG ACGAAAGGGA 
CACAAGGGCT ATCCTGGACC GGCAGGGCAC CCCGGAGAAC AGGGGCAGCC AGGACCTGAG 
GGCAGCCCAG GGGCCAAAGG TTACCCTGGC AGGCAGGGGT TACCTGGACC GGTAGGAGAT 
CCCGGCCCCA AAGGCAGCAG GGGCTACATT GGGCTCCCAG GGCTCTTCGG CCTGCCAGGG 
TCTGATGGAG AGCGAGGCCT TCCTGGTGTT CCTGGCAAGA GGGGCAAGAT GGGTATGCCG 
GGGTTTCCTG GAGTCTTTGG GGAAAGAGGC CCTCCCGGAC TGGATGGAAA CCCTGGAGAA 
CTGGGCCTGC CAGGCCCCCC TGGAGTCCCC GGCCTCATTG GTGACTTGGG AGTGTTGGGT 
CCGATTGGCT ACCCAGGACC CAAGGGCATG AAGGGACTGA TGGGCAGCGT GGGGGAGCCC 
GGACTGAAAG GTGATAAGGG TGAACAAGGG GTTCCAGGTG TGTCGGGAGA TCCCGGATTC 
CAAGGAGACA AGGGGAGCCA GGGGTTGCCA GGGTTCCCCG GCGCACGGGG GAAGCCAGGG 
CCTCTGGGCA AAGTTGGAGA CAAAGGATCC ATTGGGTTTC CCGGGCCCCC TGGACCCGAG 
GGATTCCCAG GAGACATCGG CCCCCCTGGT GACAACGGCC CAGAAGGCAT GAAGGGTAAG 
CCTGGAGCCC GAGGCCTGCC GGGACCCCGT GGGCAGCTGG GGCCCGAGGG AGATGAGGGA 
CCCATGGGGC CACCAGGGGC CCCTGGCTTG GAGGGTCAGC CTGGCAGGAA GGGCTTTCCT 
GGGAGGCCCG GCCTGGATGG CGTGAAGGGG GAACCGGGAG ATCCTGGTCG GCCAGGGCCT 
GTGGGAGAGC AGGGATTTAT GGGATTCATT GGTCTGGTCG GGGAGCCAGG AATCGTGGGA 
GAAAAGGGTG ATCGTGGCAT GATGGGACCC CCAGGCGTGC CTGGACCCAA GGGGTCGATG 
GGTCATCCTG GAATGCCAGG TGGTATGGGG ACCCCTGGAG AACCTGGACC CCAGGGTCCT 
CCAGGATCTC GAGGCCCACC AGGCATGAGG GGAGCGAAGG GACGTCGGGG CCCCCGAGGA 
CCGGACGGAC CAGCTGGGGA GCAAGGGTCC AGGGGCCTGA AGGGCCCTCC AGGACCCCAG 
GGCAGACCGG GCCGGCCTGG ACAGCAGGGT GTGGCTGGTG AGCAAGGCCA CTTGGGCTCG 
CGAGGCTTTC CCGGCATCCC GGGTCCCTCA GGCCCCCCAG GCACCAAGGG CCTCCCAGGA 
GAACCGGGCC CTCAGGGACC CCAGGGGCCA GTTGGGCCTC CAGGAGAGAT GGGACCTAAG 
GGGCCGCCGG GTGCAGCGGG AGAACCGGGC CTTCCTGGGG AAGCTGGGAT GAAGGGTGAC 
CTTGGACCCC TGGGCACCCC TGGGGAGCAG GGCCTCATTG GGCAACGGGG AGAGCCAGGC 
CTCGAGGGTG ACAGTGGCCC CATGGGACCT GATGGGCTGA AGGGGGACAG GGGAGACCCA 
GGGCCTGATG GAGAACATGG CGAGAAAGGC CAGGAAGGGC TGATGGGTGA GGACGGGCCC 
CCCGGCCCCC CTGGTGTCAC TGGTGTCCGG GGTCCTGAAG GAAAATCAGG GAAGCAAGGT 
GAGAAGGGCC GCACTGGAGC CAAGGGCGCC AAGGGCTACC AAGGACAGCT GGGTGAGATG 
GGCGTCCCTG GAGACCCCGG ACCCCCTGGC ACTCCAGGCC CTAAAGGGTC CCGGGGCAGC 
CTGGGACCAA CGGGTGCTCC TGGACGCATG GGGGCCCAAG GAGAACTGGG ACTGGCTGGT 
TATGATGGAC ACAAAGGCAT TGTGGGACCC CTCGGACCTC CTGGACCAAA AGGCGAAAAG 
GGGGAGCAGG GCGAGGACGG CAAGGCCGAG GGGCCCCCTG GGCCACCTGG AGATCGGGGC 
CCTGTGGGTG ATCGAGGAGA CCGCGGGGAA CCCGGGGACC CTGGGTACCC TGGACAGGAG 
GGTGTGCAAG GCCTCCGTGG AAAGCCAGGC CAGCAGGGCC AACCCGGGCA TCCGGGACCC 
CGGGGGCGGC CGGGACCCAA AGGATCTAAA GGCGCAGAGG GACCAAAGGG AAAGCAAGGC 
AAGGCGGGGG CCCCAGGCCG GAGGGGGGTC CAGGGCCTGC AGGGGCTGCC AGGGCCCCGG 
GGCGTGGTGG GGAGACAGGG CCTCGAGGGC ATCGCTGGAG CAGATGGGCT TCCTGGCAGG 
GACGGGCAAG TGGGACAGCA GGGGGAGCAG GGAGACGATG GGGACCCTGG CCCCATGGGC 
CCTGCTGGGA AGAGAGGAAA TCCAGGTGTG GCCGGCTTAC CTGGAGCACA GGGACCCCCA 
GGATTCAAGG GTGAGAGTGG GTTACCCGGA CAGCTGGGTC CCCCTGGCAA ACGAGGGACG 
GAGGGCAGAA CAGGGCTCCC TGGAAACCAG GGGGAGCCTG GGTCCAAAGG CCAGCCGGGC 
GACTCTGGTG AGATGGGCTT CCCAGGAATG GCAGGTCTCT TCGGACCTAA GGGCCCCCCT 
GGAGACATCG GCTTCAAAGG CATCCAGGGT CCTCGGGGGC CACCCGGTTT GATGGGAAAG 
GAAGGCATCA TCGGGCCCCT CGGAATCCTG GGACCTTCGG GACTCCCGGG TCCGAAGGGT 
GACAAAGGCA GCCGTGGGGA CTGGGGATTG CAAGGTCCGA GGGGTCCTCC TGGCCCCAGA 
GGGCGGCCCG GCCCCCCGGG TCCTCCAGGG GGTCCTATCC AATTGCAACA AGATGATCTT 
GGGGTAGCTT TCCAGACGTG GATGGACACC AGTGGAGCAC TCAGACCAGA GGGTTACAGC 
TATCCGGACC GGCTGGTGCT TGACCAGGGA GGAGAGATCT TTAAAACCTT ACACTACCTC 
AGCAACCTCA TCCAGAGCAT TAAGACGCCC CTGGGCACCA AAGAGAACCC CGCCCGGGTC 
TGCAGGGACC TCATGGACTG TGAGCAGAAG ATGGTGGATG GTACCTACTG GGTGGATCCA 
AACCTTGGCT GCTCATCCGA CACCATTGAA GTCTCCTGCA ACTTCACGCA TGGTGGACAG 
ACGTGTCTCA AACCCATCAC GGCCTCCAAG GTCGAGTTTG CCATCAGCCG GGTCCAGATG 
AATTTCCTGC ACCTGCTAAG CTCTGAGGTG ACCCAGCATA TCACCATCCA CTGCCTTAAC 
ATGACTGTGT GGCAGGAGGG CACTGGGCAG ACCCCAGCCA AGCAGGCCGT GCGCTTCCGG 
GCCTGGAACG GACAGATTTT TGAAGCTGGG GGTCAGTTCC GGCCCGAGGT GTCCATGGAT 
GGCTGCAAGG TCCAAGACGG CCGCTGGCAT CAGACACTCT TCACCTTCCG GACCCAAGAC 
CCCCAACAGC TGCCCATCGT CAGTGTGGAC AACCTCCCTC CTGCCTCATC AGGGAAGCAG 
TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028690758.1
CDS	16..5571
Translation	MPLGAGGRTDRPGSHLGTQGFLFSWILVSFACHLASTQGAPEDVDVLQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGSVIPASLGTELALVLSLCSHRVNHAFLFAVRSQKHKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPLGSQPAGRGPKGTVVPATPTKPQRTSPTNPHQHIAGRGPAQTPPAKLSASNTLDPVLPASLGSSTRTPRPAASQPSQKITATKIPKSLPTKPSAPSTSIVPVKSPHPTQKTAAPSFTKSAPPTQKHVPTVPAKVSRPAEKPIQRNQGMPRPPLPSTQPLPPTTSSSKKPIPTLAQTEAKVTNHASKPASARTSTHKPPPFTALSSSPAPTPGSTRTTRPPATMVPPTSGTSAPRTAPAVPTPGSAPTGNKKPTGSEASKKAGPESSPRKPVPLRPGKAARDVPLSDLTTRPSPRQSQPSQQTTSALVLAPAQFLSSSPRPTSSGYSFFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGEQGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPVGPPGEMGPKGPPGAAGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGELGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGRPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGADGLPGRDGQVGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGVAFQTWMDTSGALRPEGYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIVSVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_028834925.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028834925.1

ORF Insert Sequence:

ATGCCGTTAG GCGCCGGAGG AAGGACCGAC CGGCCAGGCT CGCACTTAGG AACCCAGGGG 
TTTCTCTTCT CCTGGATCTT AGTCTCGTTT GCCTGTCACC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGACGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAGGC CGGGAGCCCT 
GCACCCCCGG GAGTCATTCC TTTCCAGTCT GGCTTCATCT TTACACAGCG GGCCCGGCTC 
CAGGCTCCCA CGGGCTCCGT CATTCCTGCC TCCCTGGGCA CAGAGCTGGC GCTGGTGCTG 
AGCCTCTGCT CCCACCGGGT GAACCATGCC TTCCTCTTCG CTGTCCGCAG CCAGAAACAC 
AAGCTGCAGC TGGGCCTGCA GTTCCTCCCC GGCAAGACGG TCGTCCACCT GGGGTCCCGG 
CGTTCGGTGG CCTTCGACCT CGACATGCAC GACGGGCGCT GGCACCACCT GGCCCTCGAG 
CTCCGAGGCC GCACAGTCAC TCTGGTGACC GCCTGCGGGC AGCGCCGGGT GCCTGTCCTG 
CTGCCTTTCC ACAGGGACCC TGCACTCGAC CCCGGGGGCT CCTTCCTCTT TGGGAAGATG 
AACCCGCATG CAGTCCAGTT TGAAGGTGCT CTGTGCCAGT TCAGTATCTA CCCTGTGACG 
CAGGTCGCTC ACAATTACTG TACCCACCTG AGGAAGCAGT GTGGACAGGC TGACACGTAC 
CAGTCCCCAC TGGGGCCTCT CTTCTCCCAA GACTCTGGCA GACCTTTCAC CTTCCAGTCT 
GACCTCGCCC TGCTAGGCCT GGAGAACTTG ACCACTGCCA CACCACTGGG GTCACAGCCA 
GCAGGCAGGG GACCCAAGGG GACTGTGGTG CCCGCCACTC CCACCAAGCC CCAAAGGACT 
AGCCCCACAA ACCCTCACCA GCATATCGCG GGGCGAGGCC CAGCCCAAAC CCCACCTGCC 
AAGCTGTCAG CCAGTAACAC ACTTGATCCT GTGCTCCCAG CCTCTCTTGG CAGCTCTACC 
AGAACGCCTC GCCCTGCAGC CTCTCAACCA TCACAGAAGA TCACAGCCAC CAAAATCCCC 
AAAAGCCTCC CTACCAAGCC TTCAGCCCCT TCTACTTCAA TTGTCCCCGT CAAAAGTCCC 
CATCCTACCC AGAAAACAGC TGCACCTTCA TTTACAAAGT CAGCCCCACC CACTCAGAAG 
CATGTACCTA CAGTTCCTGC CAAAGTATCC CGTCCCGCAG AGAAGCCCAT CCAGAGGAAC 
CAAGGAATGC CCAGGCCCCC ACTGCCCAGC ACCCAGCCCC TACCTCCTAC CACCAGCTCC 
TCTAAAAAAC CCATTCCCAC ACTAGCTCAA ACTGAGGCCA AGGTGACTAA CCATGCCAGT 
AAGCCGGCCT CTGCCCGCAC CAGCACCCAC AAACCTCCCC CATTTACTGC TTTATCCTCA 
TCTCCTGCCC CTACTCCTGG TTCTACCAGG ACGACTCGGC CACCAGCCAC AATGGTGCCT 
CCAACCTCGG GCACCAGCGC TCCCAGAACA GCACCTGCCG TCCCCACTCC TGGCTCAGCT 
CCCACTGGAA ACAAGAAGCC CACTGGATCG GAAGCCTCAA AGAAAGCCGG ACCTGAGAGC 
AGCCCCCGGA AGCCCGTCCC CCTCAGACCT GGGAAGGCAG CCAGGGATGT CCCCTTGAGC 
GATCTGACAA CCAGGCCTAG CCCCAGACAG TCCCAGCCCA GTCAGCAGAC CACTTCAGCC 
CTGGTATTGG CCCCAGCTCA ATTCCTGTCC TCCAGCCCCC GGCCCACGAG CAGTGGCTAT 
TCGTTCTTCC ACCTGGCAGG ATCTACGCCT TTCCCTCTGC TGATGGGGCC TCCGGGGCCC 
AAGGGAGACT GTGGTTTGCC GGGTCCCCCT GGGCTGCCTG GGCTACCTGG AATCCCTGGT 
GCACGTGGGC CTCGGGGTCC TCCTGGGCCT TATGGAAATC CAGGTCTCCC CGGCCCTCCT 
GGAGCCAAAG GACAGAAAGG GGACCCAGGG CTCTCACCAG GAAAGGCCCA CGATGGGGCA 
AAGGGCGACA TGGGCTTGCC TGGGCTGTCC GGGAATCCAG GACCTCCGGG ACGAAAGGGA 
CACAAGGGCT ATCCTGGACC GGCAGGGCAC CCCGGAGAAC AGGGGCAGCC AGGACCTGAG 
GGCAGCCCAG GGGCCAAAGG TTACCCTGGC AGGCAGGGGT TACCTGGACC GGTAGGAGAT 
CCCGGCCCCA AAGGCAGCAG GGGCTACATT GGGCTCCCAG GGCTCTTCGG CCTGCCAGGG 
TCTGATGGAG AGCGAGGCCT TCCTGGTGTT CCTGGCAAGA GGGGCAAGAT GGGTATGCCG 
GGGTTTCCTG GAGTCTTTGG GGAAAGAGGC CCTCCCGGAC TGGATGGAAA CCCTGGAGAA 
CTGGGCCTGC CAGGCCCCCC TGGAGTCCCC GGCCTCATTG GTGACTTGGG AGTGTTGGGT 
CCGATTGGCT ACCCAGGACC CAAGGGCATG AAGGGACTGA TGGGCAGCGT GGGGGAGCCC 
GGACTGAAAG GTGATAAGGG TGAACAAGGG GTTCCAGGTG TGTCGGGAGA TCCCGGATTC 
CAAGGAGACA AGGGGAGCCA GGGGTTGCCA GGGTTCCCCG GCGCACGGGG GAAGCCAGGG 
CCTCTGGGCA AAGTTGGAGA CAAAGGATCC ATTGGGTTTC CCGGGCCCCC TGGACCCGAG 
GGATTCCCAG GAGACATCGG CCCCCCTGGT GACAACGGCC CAGAAGGCAT GAAGGGTAAG 
CCTGGAGCCC GAGGCCTGCC GGGACCCCGT GGGCAGCTGG GGCCCGAGGG AGATGAGGGA 
CCCATGGGGC CACCAGGGGC CCCTGGCTTG GAGGGTCAGC CTGGCAGGAA GGGCTTTCCT 
GGGAGGCCCG GCCTGGATGG CGTGAAGGGG GAACCGGGAG ATCCTGGTCG GCCAGGGCCT 
GTGGGAGAGC AGGGATTTAT GGGATTCATT GGTCTGGTCG GGGAGCCAGG AATCGTGGGA 
GAAAAGGGTG ATCGTGGCAT GATGGGACCC CCAGGCGTGC CTGGACCCAA GGGGTCGATG 
GGTCATCCTG GAATGCCAGG TGGTATGGGG ACCCCTGGAG AACCTGGACC CCAGGGTCCT 
CCAGGATCTC GAGGCCCACC AGGCATGAGG GGAGCGAAGG GACGTCGGGG CCCCCGAGGA 
CCGGACGGAC CAGCTGGGGA GCAAGGGTCC AGGGGCCTGA AGGGCCCTCC AGGACCCCAG 
GGCAGACCGG GCCGGCCTGG ACAGCAGGGT GTGGCTGGTG AGCAAGGCCA CTTGGGCTCG 
CGAGGCTTTC CCGGCATCCC GGGTCCCTCA GGCCCCCCAG GCACCAAGGG CCTCCCAGGA 
GAACCGGGCC CTCAGGGACC CCAGGGGCCA GTTGGGCCTC CAGGAGAGAT GGGACCTAAG 
GGGCCGCCGG GTGCAGCGGG AGAACCGGGC CTTCCTGGGG AAGCTGGGAT GAAGGGTGAC 
CTTGGACCCC TGGGCACCCC TGGGGAGCAG GGCCTCATTG GGCAACGGGG AGAGCCAGGC 
CTCGAGGGTG ACAGTGGCCC CATGGGACCT GATGGGCTGA AGGGGGACAG GGGAGACCCA 
GGGCCTGATG GAGAACATGG CGAGAAAGGC CAGGAAGGGC TGATGGGTGA GGACGGGCCC 
CCCGGCCCCC CTGGTGTCAC TGGTGTCCGG GGTCCTGAAG GAAAATCAGG GAAGCAAGGT 
GAGAAGGGCC GCACTGGAGC CAAGGGCGCC AAGGGCTACC AAGGACAGCT GGGTGAGATG 
GGCGTCCCTG GAGACCCCGG ACCCCCTGGC ACTCCAGGCC CTAAAGGGTC CCGGGGCAGC 
CTGGGACCAA CGGGTGCTCC TGGACGCATG GGGGCCCAAG GAGAACTGGG ACTGGCTGGT 
TATGATGGAC ACAAAGGCAT TGTGGGACCC CTCGGACCTC CTGGACCAAA AGGCGAAAAG 
GGGGAGCAGG GCGAGGACGG CAAGGCCGAG GGGCCCCCTG GGCCACCTGG AGATCGGGGC 
CCTGTGGGTG ATCGAGGAGA CCGCGGGGAA CCCGGGGACC CTGGGTACCC TGGACAGGAG 
GGTGTGCAAG GCCTCCGTGG AAAGCCAGGC CAGCAGGGCC AACCCGGGCA TCCGGGACCC 
CGGGGGCGGC CGGGACCCAA AGGATCTAAA GGCGCAGAGG GACCAAAGGG AAAGCAAGGC 
AAGGCGGGGG CCCCAGGCCG GAGGGGGGTC CAGGGCCTGC AGGGGCTGCC AGGGCCCCGG 
GGCGTGGTGG GGAGACAGGG CCTCGAGGGC ATCGCTGGAG CAGATGGGCT TCCTGGCAGG 
GACGGGCAAG TGGGACAGCA GGGGGAGCAG GGAGACGATG GGGACCCTGG CCCCATGGGC 
CCTGCTGGGA AGAGAGGAAA TCCAGGTGTG GCCGGCTTAC CTGGAGCACA GGGACCCCCA 
GGATTCAAGG GTGAGAGTGG GTTACCCGGA CAGCTGGGTC CCCCTGGCAA ACGAGGGACG 
GAGGGCAGAA CAGGGCTCCC TGGAAACCAG GGGGAGCCTG GGTCCAAAGG CCAGCCGGGC 
GACTCTGGTG AGATGGGCTT CCCAGGAATG GCAGGTCTCT TCGGACCTAA GGGCCCCCCT 
GGAGACATCG GCTTCAAAGG CATCCAGGGT CCTCGGGGGC CACCCGGTTT GATGGGAAAG 
GAAGGCATCA TCGGGCCCCT CGGAATCCTG GGACCTTCGG GACTCCCGGG TCCGAAGGGT 
GACAAAGGCA GCCGTGGGGA CTGGGGATTG CAAGGTCCGA GGGGTCCTCC TGGCCCCAGA 
GGGCGGCCCG GCCCCCCGGG TCCTCCAGGG GGTCCTATCC AATTGCAACA AGATGATCTT 
GGGGTAGCTT TCCAGACGTG GATGGACACC AGTGGAGCAC TCAGACCAGA GGGTTACAGC 
TATCCGGACC GGCTGGTGCT TGACCAGGGA GGAGAGATCT TTAAAACCTT ACACTACCTC 
AGCAACCTCA TCCAGAGCAT TAAGACGCCC CTGGGCACCA AAGAGAACCC CGCCCGGGTC 
TGCAGGGACC TCATGGACTG TGAGCAGAAG ATGGTGGATG GTACCTACTG GGTGGATCCA 
AACCTTGGCT GCTCATCCGA CACCATTGAA GTCTCCTGCA ACTTCACGCA TGGTGGACAG 
ACGTGTCTCA AACCCATCAC GGCCTCCAAG GTCGAGTTTG CCATCAGCCG GGTCCAGATG 
AATTTCCTGC ACCTGCTAAG CTCTGAGGTG ACCCAGCATA TCACCATCCA CTGCCTTAAC 
ATGACTGTGT GGCAGGAGGG CACTGGGCAG ACCCCAGCCA AGCAGGCCGT GCGCTTCCGG 
GCCTGGAACG GACAGATTTT TGAAGCTGGG GGTCAGTTCC GGCCCGAGGT GTCCATGGAT 
GGCTGCAAGG TCCAAGACGG CCGCTGGCAT CAGACACTCT TCACCTTCCG GACCCAAGAC 
CCCCAACAGC TGCCCATCGT CAGTGTGGAC AACCTCCCTC CTGCCTCATC AGGGAAGCAG 
TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_028834926.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028690759.1 Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.

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CloneID	OMb71340
Clone ID Related Accession (Same CDS sequence)	XM_028834926.1
Accession Version	XM_028834926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	collagen alpha-1(XXVII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGGGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGTCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAAACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGACA GAAAGGGGAC CCAGGGCTCT CACCAGGAAA GGCCCACGAT 
GGGGCAAAGG GCGACATGGG CTTGCCTGGG CTGTCCGGGA ATCCAGGACC TCCGGGACGA 
AAGGGACACA AGGGCTATCC TGGACCGGCA GGGCACCCCG GAGAACAGGG GCAGCCAGGA 
CCTGAGGGCA GCCCAGGGGC CAAAGGTTAC CCTGGCAGGC AGGGGTTACC TGGACCGGTA 
GGAGATCCCG GCCCCAAAGG CAGCAGGGGC TACATTGGGC TCCCAGGGCT CTTCGGCCTG 
CCAGGGTCTG ATGGAGAGCG AGGCCTTCCT GGTGTTCCTG GCAAGAGGGG CAAGATGGGT 
ATGCCGGGGT TTCCTGGAGT CTTTGGGGAA AGAGGCCCTC CCGGACTGGA TGGAAACCCT 
GGAGAACTGG GCCTGCCAGG CCCCCCTGGA GTCCCCGGCC TCATTGGTGA CTTGGGAGTG 
TTGGGTCCGA TTGGCTACCC AGGACCCAAG GGCATGAAGG GACTGATGGG CAGCGTGGGG 
GAGCCCGGAC TGAAAGGTGA TAAGGGTGAA CAAGGGGTTC CAGGTGTGTC GGGAGATCCC 
GGATTCCAAG GAGACAAGGG GAGCCAGGGG TTGCCAGGGT TCCCCGGCGC ACGGGGGAAG 
CCAGGGCCTC TGGGCAAAGT TGGAGACAAA GGATCCATTG GGTTTCCCGG GCCCCCTGGA 
CCCGAGGGAT TCCCAGGAGA CATCGGCCCC CCTGGTGACA ACGGCCCAGA AGGCATGAAG 
GGAGATGAGG GACCCATGGG GCCACCAGGG GCCCCTGGCT TGGAGGGTCA GCCTGGCAGG 
AAGGGCTTTC CTGGGAGGCC CGGCCTGGAT GGCGTGAAGG GGGAACCGGG AGATCCTGGT 
CGGCCAGGGC CTGTGGGAGA GCAGGGATTT ATGGGATTCA TTGGTCTGGT CGGGGAGCCA 
GGAATCGTGG GAGAAAAGGG TGATCGTGGC ATGATGGGAC CCCCAGGCGT GCCTGGACCC 
AAGGGGTCGA TGGGTCATCC TGGAATGCCA GGTGGTATGG GGACCCCTGG AGAACCTGGA 
CCCCAGGGTC CTCCAGGATC TCGAGGCCCA CCAGGCATGA GGGGAGCGAA GGGACGTCGG 
GGCCCCCGAG GACCGGACGG ACCAGCTGGG GAGCAAGGGT CCAGGGGCCT GAAGGGCCCT 
CCAGGACCCC AGGGCAGACC GGGCCGGCCT GGACAGCAGG GTGTGGCTGG TGAGCAAGGC 
CACTTGGGCT CGCGAGGCTT TCCCGGCATC CCGGGTCCCT CAGGCCCCCC AGGCACCAAG 
GGCCTCCCAG GAGAACCGGG CCCTCAGGGA CCCCAGGGGC CAGTTGGGCC TCCAGGAGAG 
ATGGGACCTA AGGGGCCGCC GGGTGCAGCG GGAGAACCGG GCCTTCCTGG GGAAGCTGGG 
ATGAAGGGTG ACCTTGGACC CCTGGGCACC CCTGGGGAGC AGGGCCTCAT TGGGCAACGG 
GGAGAGCCAG GCCTCGAGGG TGACAGTGGC CCCATGGGAC CTGATGGGCT GAAGGGGGAC 
AGGGGAGACC CAGGGCCTGA TGGAGAACAT GGCGAGAAAG GCCAGGAAGG GCTGATGGGT 
GAGGACGGGC CCCCCGGCCC CCCTGGTGTC ACTGGTGTCC GGGGTCCTGA AGGAAAATCA 
GGGAAGCAAG GTGAGAAGGG CCGCACTGGA GCCAAGGGCG CCAAGGGCTA CCAAGGACAG 
CTGGGTGAGA TGGGCGTCCC TGGAGACCCC GGACCCCCTG GCACTCCAGG CCCTAAAGGG 
TCCCGGGGCA GCCTGGGACC AACGGGTGCT CCTGGACGCA TGGGGGCCCA AGGAGAACTG 
GGACTGGCTG GTTATGATGG ACACAAAGGC ATTGTGGGAC CCCTCGGACC TCCTGGACCA 
AAAGGCGAAA AGGGGGAGCA GGGCGAGGAC GGCAAGGCCG AGGGGCCCCC TGGGCCACCT 
GGAGATCGGG GCCCTGTGGG TGATCGAGGA GACCGCGGGG AACCCGGGGA CCCTGGGTAC 
CCTGGACAGG AGGGTGTGCA AGGCCTCCGT GGAAAGCCAG GCCAGCAGGG CCAACCCGGG 
CATCCGGGAC CCCGGGGGCG GCCGGGACCC AAAGGATCTA AAGGCGCAGA GGGACCAAAG 
GGAAAGCAAG GCAAGGCGGG GGCCCCAGGC CGGAGGGGGG TCCAGGGCCT GCAGGGGCTG 
CCAGGGCCCC GGGGCGTGGT GGGGAGACAG GGCCTCGAGG GCATCGCTGG AGCAGATGGG 
CTTCCTGGCA GGGACGGGCA AGTGGGACAG CAGGGGGAGC AGGGAGACGA TGGGGACCCT 
GGCCCCATGG GCCCTGCTGG GAAGAGAGGA AATCCAGGTG TGGCCGGCTT ACCTGGAGCA 
CAGGGACCCC CAGGATTCAA GGGTGAGAGT GGGTTACCCG GACAGCTGGG TCCCCCTGGC 
AAACGAGGGA CGGAGGGCAG AACAGGGCTC CCTGGAAACC AGGGGGAGCC TGGGTCCAAA 
GGCCAGCCGG GCGACTCTGG TGAGATGGGC TTCCCAGGAA TGGCAGGTCT CTTCGGACCT 
AAGGGCCCCC CTGGAGACAT CGGCTTCAAA GGCATCCAGG GTCCTCGGGG GCCACCCGGT 
TTGATGGGAA AGGAAGGCAT CATCGGGCCC CTCGGAATCC TGGGACCTTC GGGACTCCCG 
GGTCCGAAGG GTGACAAAGG CAGCCGTGGG GACTGGGGAT TGCAAGGTCC GAGGGGTCCT 
CCTGGCCCCA GAGGGCGGCC CGGCCCCCCG GGTCCTCCAG GGGGTCCTAT CCAATTGCAA 
CAAGATGATC TTGGGGTAGC TTTCCAGACG TGGATGGACA CCAGTGGAGC ACTCAGACCA 
GAGGGTTACA GCTATCCGGA CCGGCTGGTG CTTGACCAGG GAGGAGAGAT CTTTAAAACC 
TTACACTACC TCAGCAACCT CATCCAGAGC ATTAAGACGC CCCTGGGCAC CAAAGAGAAC 
CCCGCCCGGG TCTGCAGGGA CCTCATGGAC TGTGAGCAGA AGATGGTGGA TGGTACCTAC 
TGGGTGGATC CAAACCTTGG CTGCTCATCC GACACCATTG AAGTCTCCTG CAACTTCACG 
CATGGTGGAC AGACGTGTCT CAAACCCATC ACGGCCTCCA AGGTCGAGTT TGCCATCAGC 
CGGGTCCAGA TGAATTTCCT GCACCTGCTA AGCTCTGAGG TGACCCAGCA TATCACCATC 
CACTGCCTTA ACATGACTGT GTGGCAGGAG GGCACTGGGC AGACCCCAGC CAAGCAGGCC 
GTGCGCTTCC GGGCCTGGAA CGGACAGATT TTTGAAGCTG GGGGTCAGTT CCGGCCCGAG 
GTGTCCATGG ATGGCTGCAA GGTCCAAGAC GGCCGCTGGC ATCAGACACT CTTCACCTTC 
CGGACCCAAG ACCCCCAACA GCTGCCCATC GTCAGTGTGG ACAACCTCCC TCCTGCCTCA 
TCAGGGAAGC AGTACCGCCT GGAAGTTGGA CCTGCGTGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028690759.1
CDS	459..5966
Translation	MGAGSARGARGTATAAAAHGGGFLFSWILVSFACHLASTQGAPEDVDVLQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGSVIPASLGTELALVLSLCSHRVNHAFLFAVRSQKHKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPLGSQPAGRGPKGTVVPATPTKPQRTSPTNPHQHIAGRGPAQTPPAKLSASNTLDPVLPASLGSSTRTPRPAASQPSQKITATKIPKSLPTKPSAPSTSIVPVKSPHPTQKTAAPSFTKSAPPTQKHVPTVPAKVSRPAEKPIQRNQGMPRPPLPSTQPLPPTTSSSKKPIPTLAQTEAKVTNHASKPASARTSTHKPPPFTALSSSPAPTPGSTRTTRPPATMVPPTSGTSAPRTAPAVPTPGSAPTGNKKPTGSEASKKAGPESSPRKPVPLRPGKAARDVPLSDLTTRPSPRQSQPSQQTTSALVLAPAQFLSSSPRPTSSGYSFFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGEQGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPVGPPGEMGPKGPPGAAGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGELGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGRPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGADGLPGRDGQVGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGVAFQTWMDTSGALRPEGYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIVSVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_028834926.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028834926.1

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGGGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGTCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAAACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGACA GAAAGGGGAC CCAGGGCTCT CACCAGGAAA GGCCCACGAT 
GGGGCAAAGG GCGACATGGG CTTGCCTGGG CTGTCCGGGA ATCCAGGACC TCCGGGACGA 
AAGGGACACA AGGGCTATCC TGGACCGGCA GGGCACCCCG GAGAACAGGG GCAGCCAGGA 
CCTGAGGGCA GCCCAGGGGC CAAAGGTTAC CCTGGCAGGC AGGGGTTACC TGGACCGGTA 
GGAGATCCCG GCCCCAAAGG CAGCAGGGGC TACATTGGGC TCCCAGGGCT CTTCGGCCTG 
CCAGGGTCTG ATGGAGAGCG AGGCCTTCCT GGTGTTCCTG GCAAGAGGGG CAAGATGGGT 
ATGCCGGGGT TTCCTGGAGT CTTTGGGGAA AGAGGCCCTC CCGGACTGGA TGGAAACCCT 
GGAGAACTGG GCCTGCCAGG CCCCCCTGGA GTCCCCGGCC TCATTGGTGA CTTGGGAGTG 
TTGGGTCCGA TTGGCTACCC AGGACCCAAG GGCATGAAGG GACTGATGGG CAGCGTGGGG 
GAGCCCGGAC TGAAAGGTGA TAAGGGTGAA CAAGGGGTTC CAGGTGTGTC GGGAGATCCC 
GGATTCCAAG GAGACAAGGG GAGCCAGGGG TTGCCAGGGT TCCCCGGCGC ACGGGGGAAG 
CCAGGGCCTC TGGGCAAAGT TGGAGACAAA GGATCCATTG GGTTTCCCGG GCCCCCTGGA 
CCCGAGGGAT TCCCAGGAGA CATCGGCCCC CCTGGTGACA ACGGCCCAGA AGGCATGAAG 
GGAGATGAGG GACCCATGGG GCCACCAGGG GCCCCTGGCT TGGAGGGTCA GCCTGGCAGG 
AAGGGCTTTC CTGGGAGGCC CGGCCTGGAT GGCGTGAAGG GGGAACCGGG AGATCCTGGT 
CGGCCAGGGC CTGTGGGAGA GCAGGGATTT ATGGGATTCA TTGGTCTGGT CGGGGAGCCA 
GGAATCGTGG GAGAAAAGGG TGATCGTGGC ATGATGGGAC CCCCAGGCGT GCCTGGACCC 
AAGGGGTCGA TGGGTCATCC TGGAATGCCA GGTGGTATGG GGACCCCTGG AGAACCTGGA 
CCCCAGGGTC CTCCAGGATC TCGAGGCCCA CCAGGCATGA GGGGAGCGAA GGGACGTCGG 
GGCCCCCGAG GACCGGACGG ACCAGCTGGG GAGCAAGGGT CCAGGGGCCT GAAGGGCCCT 
CCAGGACCCC AGGGCAGACC GGGCCGGCCT GGACAGCAGG GTGTGGCTGG TGAGCAAGGC 
CACTTGGGCT CGCGAGGCTT TCCCGGCATC CCGGGTCCCT CAGGCCCCCC AGGCACCAAG 
GGCCTCCCAG GAGAACCGGG CCCTCAGGGA CCCCAGGGGC CAGTTGGGCC TCCAGGAGAG 
ATGGGACCTA AGGGGCCGCC GGGTGCAGCG GGAGAACCGG GCCTTCCTGG GGAAGCTGGG 
ATGAAGGGTG ACCTTGGACC CCTGGGCACC CCTGGGGAGC AGGGCCTCAT TGGGCAACGG 
GGAGAGCCAG GCCTCGAGGG TGACAGTGGC CCCATGGGAC CTGATGGGCT GAAGGGGGAC 
AGGGGAGACC CAGGGCCTGA TGGAGAACAT GGCGAGAAAG GCCAGGAAGG GCTGATGGGT 
GAGGACGGGC CCCCCGGCCC CCCTGGTGTC ACTGGTGTCC GGGGTCCTGA AGGAAAATCA 
GGGAAGCAAG GTGAGAAGGG CCGCACTGGA GCCAAGGGCG CCAAGGGCTA CCAAGGACAG 
CTGGGTGAGA TGGGCGTCCC TGGAGACCCC GGACCCCCTG GCACTCCAGG CCCTAAAGGG 
TCCCGGGGCA GCCTGGGACC AACGGGTGCT CCTGGACGCA TGGGGGCCCA AGGAGAACTG 
GGACTGGCTG GTTATGATGG ACACAAAGGC ATTGTGGGAC CCCTCGGACC TCCTGGACCA 
AAAGGCGAAA AGGGGGAGCA GGGCGAGGAC GGCAAGGCCG AGGGGCCCCC TGGGCCACCT 
GGAGATCGGG GCCCTGTGGG TGATCGAGGA GACCGCGGGG AACCCGGGGA CCCTGGGTAC 
CCTGGACAGG AGGGTGTGCA AGGCCTCCGT GGAAAGCCAG GCCAGCAGGG CCAACCCGGG 
CATCCGGGAC CCCGGGGGCG GCCGGGACCC AAAGGATCTA AAGGCGCAGA GGGACCAAAG 
GGAAAGCAAG GCAAGGCGGG GGCCCCAGGC CGGAGGGGGG TCCAGGGCCT GCAGGGGCTG 
CCAGGGCCCC GGGGCGTGGT GGGGAGACAG GGCCTCGAGG GCATCGCTGG AGCAGATGGG 
CTTCCTGGCA GGGACGGGCA AGTGGGACAG CAGGGGGAGC AGGGAGACGA TGGGGACCCT 
GGCCCCATGG GCCCTGCTGG GAAGAGAGGA AATCCAGGTG TGGCCGGCTT ACCTGGAGCA 
CAGGGACCCC CAGGATTCAA GGGTGAGAGT GGGTTACCCG GACAGCTGGG TCCCCCTGGC 
AAACGAGGGA CGGAGGGCAG AACAGGGCTC CCTGGAAACC AGGGGGAGCC TGGGTCCAAA 
GGCCAGCCGG GCGACTCTGG TGAGATGGGC TTCCCAGGAA TGGCAGGTCT CTTCGGACCT 
AAGGGCCCCC CTGGAGACAT CGGCTTCAAA GGCATCCAGG GTCCTCGGGG GCCACCCGGT 
TTGATGGGAA AGGAAGGCAT CATCGGGCCC CTCGGAATCC TGGGACCTTC GGGACTCCCG 
GGTCCGAAGG GTGACAAAGG CAGCCGTGGG GACTGGGGAT TGCAAGGTCC GAGGGGTCCT 
CCTGGCCCCA GAGGGCGGCC CGGCCCCCCG GGTCCTCCAG GGGGTCCTAT CCAATTGCAA 
CAAGATGATC TTGGGGTAGC TTTCCAGACG TGGATGGACA CCAGTGGAGC ACTCAGACCA 
GAGGGTTACA GCTATCCGGA CCGGCTGGTG CTTGACCAGG GAGGAGAGAT CTTTAAAACC 
TTACACTACC TCAGCAACCT CATCCAGAGC ATTAAGACGC CCCTGGGCAC CAAAGAGAAC 
CCCGCCCGGG TCTGCAGGGA CCTCATGGAC TGTGAGCAGA AGATGGTGGA TGGTACCTAC 
TGGGTGGATC CAAACCTTGG CTGCTCATCC GACACCATTG AAGTCTCCTG CAACTTCACG 
CATGGTGGAC AGACGTGTCT CAAACCCATC ACGGCCTCCA AGGTCGAGTT TGCCATCAGC 
CGGGTCCAGA TGAATTTCCT GCACCTGCTA AGCTCTGAGG TGACCCAGCA TATCACCATC 
CACTGCCTTA ACATGACTGT GTGGCAGGAG GGCACTGGGC AGACCCCAGC CAAGCAGGCC 
GTGCGCTTCC GGGCCTGGAA CGGACAGATT TTTGAAGCTG GGGGTCAGTT CCGGCCCGAG 
GTGTCCATGG ATGGCTGCAA GGTCCAAGAC GGCCGCTGGC ATCAGACACT CTTCACCTTC 
CGGACCCAAG ACCCCCAACA GCTGCCCATC GTCAGTGTGG ACAACCTCCC TCCTGCCTCA 
TCAGGGAAGC AGTACCGCCT GGAAGTTGGA CCTGCGTGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_015117010.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014972496.2 Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X4, mRNA.

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CloneID	OMb71341
Clone ID Related Accession (Same CDS sequence)	XM_015117010.2
Accession Version	XM_015117010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	collagen alpha-1(XXVII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015117010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGGGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGTCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAAACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGCGA CATGGGCTTG CCTGGGCTGT CCGGGAATCC AGGACCTCCG 
GGACGAAAGG GACACAAGGG CTATCCTGGA CCGGCAGGGC ACCCCGGAGA ACAGGGGCAG 
CCAGGACCTG AGGGCAGCCC AGGGGCCAAA GGTTACCCTG GCAGGCAGGG GTTACCTGGA 
CCGGTAGGAG ATCCCGGCCC CAAAGGCAGC AGGGGCTACA TTGGGCTCCC AGGGCTCTTC 
GGCCTGCCAG GGTCTGATGG AGAGCGAGGC CTTCCTGGTG TTCCTGGCAA GAGGGGCAAG 
ATGGGTATGC CGGGGTTTCC TGGAGTCTTT GGGGAAAGAG GCCCTCCCGG ACTGGATGGA 
AACCCTGGAG AACTGGGCCT GCCAGGCCCC CCTGGAGTCC CCGGCCTCAT TGGTGACTTG 
GGAGTGTTGG GTCCGATTGG CTACCCAGGA CCCAAGGGCA TGAAGGGACT GATGGGCAGC 
GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTTCCAGG TGTGTCGGGA 
GATCCCGGAT TCCAAGGAGA CAAGGGGAGC CAGGGGTTGC CAGGGTTCCC CGGCGCACGG 
GGGAAGCCAG GGCCTCTGGG CAAAGTTGGA GACAAAGGAT CCATTGGGTT TCCCGGGCCC 
CCTGGACCCG AGGGATTCCC AGGAGACATC GGCCCCCCTG GTGACAACGG CCCAGAAGGC 
ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCGGGACCCC GTGGGCAGCT GGGGCCCGAG 
GGAGATGAGG GACCCATGGG GCCACCAGGG GCCCCTGGCT TGGAGGGTCA GCCTGGCAGG 
AAGGGCTTTC CTGGGAGGCC CGGCCTGGAT GGCGTGAAGG GGGAACCGGG AGATCCTGGT 
CGGCCAGGGC CTGTGGGAGA GCAGGGATTT ATGGGATTCA TTGGTCTGGT CGGGGAGCCA 
GGAATCGTGG GAGAAAAGGG TGATCGTGGC ATGATGGGAC CCCCAGGCGT GCCTGGACCC 
AAGGGGTCGA TGGGTCATCC TGGAATGCCA GGTGGTATGG GGACCCCTGG AGAACCTGGA 
CCCCAGGGTC CTCCAGGATC TCGAGGCCCA CCAGGCATGA GGGGAGCGAA GGGACGTCGG 
GGCCCCCGAG GACCGGACGG ACCAGCTGGG GAGCAAGGGT CCAGGGGCCT GAAGGGCCCT 
CCAGGACCCC AGGGCAGACC GGGCCGGCCT GGACAGCAGG GTGTGGCTGG TGAGCAAGGC 
CACTTGGGCT CGCGAGGCTT TCCCGGCATC CCGGGTCCCT CAGGCCCCCC AGGCACCAAG 
GGCCTCCCAG GAGAACCGGG CCCTCAGGGA CCCCAGGGGC CAGTTGGGCC TCCAGGAGAG 
ATGGGACCTA AGGGGCCGCC GGGTGCAGCG GGAGAACCGG GCCTTCCTGG GGAAGCTGGG 
ATGAAGGGTG ACCTTGGACC CCTGGGCACC CCTGGGGAGC AGGGCCTCAT TGGGCAACGG 
GGAGAGCCAG GCCTCGAGGG TGACAGTGGC CCCATGGGAC CTGATGGGCT GAAGGGGGAC 
AGGGGAGACC CAGGGCCTGA TGGAGAACAT GGCGAGAAAG GCCAGGAAGG GCTGATGGGT 
GAGGACGGGC CCCCCGGCCC CCCTGGTGTC ACTGGTGTCC GGGGTCCTGA AGGAAAATCA 
GGGAAGCAAG GTGAGAAGGG CCGCACTGGA GCCAAGGGCG CCAAGGGCTA CCAAGGACAG 
CTGGGTGAGA TGGGCGTCCC TGGAGACCCC GGACCCCCTG GCACTCCAGG CCCTAAAGGG 
TCCCGGGGCA GCCTGGGACC AACGGGTGCT CCTGGACGCA TGGGGGCCCA AGGAGAACTG 
GGACTGGCTG GTTATGATGG ACACAAAGGC ATTGTGGGAC CCCTCGGACC TCCTGGACCA 
AAAGGCGAAA AGGGGGAGCA GGGCGAGGAC GGCAAGGCCG AGGGGCCCCC TGGGCCACCT 
GGAGATCGGG GCCCTGTGGG TGATCGAGGA GACCGCGGGG AACCCGGGGA CCCTGGGTAC 
CCTGGACAGG AGGGTGTGCA AGGCCTCCGT GGAAAGCCAG GCCAGCAGGG CCAACCCGGG 
CATCCGGGAC CCCGGGGGCG GCCGGGACCC AAAGGATCTA AAGGCGCAGA GGGACCAAAG 
GGAAAGCAAG GCAAGGCGGG GGCCCCAGGC CGGAGGGGGG TCCAGGGCCT GCAGGGGCTG 
CCAGGGCCCC GGGGCGTGGT GGGGAGACAG GGCCTCGAGG GCATCGCTGG AGCAGATGGG 
CTTCCTGGCA GGGACGGGCA AGTGGGACAG CAGGGGGAGC AGGGAGACGA TGGGGACCCT 
GGCCCCATGG GCCCTGCTGG GAAGAGAGGA AATCCAGGTG TGGCCGGCTT ACCTGGAGCA 
CAGGGACCCC CAGGATTCAA GGGTGAGAGT GGGTTACCCG GACAGCTGGG TCCCCCTGGC 
AAACGAGGGA CGGAGGGCAG AACAGGGCTC CCTGGAAACC AGGGGGAGCC TGGGTCCAAA 
GGCCAGCCGG GCGACTCTGG TGAGATGGGC TTCCCAGGAA TGGCAGGTCT CTTCGGACCT 
AAGGGCCCCC CTGGAGACAT CGGCTTCAAA GGCATCCAGG GTCCTCGGGG GCCACCCGGT 
TTGATGGGAA AGGAAGGCAT CATCGGGCCC CTCGGAATCC TGGGACCTTC GGGACTCCCG 
GGTCCGAAGG GTGACAAAGG CAGCCGTGGG GACTGGGGAT TGCAAGGTCC GAGGGGTCCT 
CCTGGCCCCA GAGGGCGGCC CGGCCCCCCG GGTCCTCCAG GGGGTCCTAT CCAATTGCAA 
CAAGATGATC TTGGGGTAGC TTTCCAGACG TGGATGGACA CCAGTGGAGC ACTCAGACCA 
GAGGGTTACA GCTATCCGGA CCGGCTGGTG CTTGACCAGG GAGGAGAGAT CTTTAAAACC 
TTACACTACC TCAGCAACCT CATCCAGAGC ATTAAGACGC CCCTGGGCAC CAAAGAGAAC 
CCCGCCCGGG TCTGCAGGGA CCTCATGGAC TGTGAGCAGA AGATGGTGGA TGGTACCTAC 
TGGGTGGATC CAAACCTTGG CTGCTCATCC GACACCATTG AAGTCTCCTG CAACTTCACG 
CATGGTGGAC AGACGTGTCT CAAACCCATC ACGGCCTCCA AGGTCGAGTT TGCCATCAGC 
CGGGTCCAGA TGAATTTCCT GCACCTGCTA AGCTCTGAGG TGACCCAGCA TATCACCATC 
CACTGCCTTA ACATGACTGT GTGGCAGGAG GGCACTGGGC AGACCCCAGC CAAGCAGGCC 
GTGCGCTTCC GGGCCTGGAA CGGACAGATT TTTGAAGCTG GGGGTCAGTT CCGGCCCGAG 
GTGTCCATGG ATGGCTGCAA GGTCCAAGAC GGCCGCTGGC ATCAGACACT CTTCACCTTC 
CGGACCCAAG ACCCCCAACA GCTGCCCATC GTCAGTGTGG ACAACCTCCC TCCTGCCTCA 
TCAGGGAAGC AGTACCGCCT GGAAGTTGGA CCTGCGTGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014972496.2
CDS	459..5966
Translation	MGAGSARGARGTATAAAAHGGGFLFSWILVSFACHLASTQGAPEDVDVLQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGSVIPASLGTELALVLSLCSHRVNHAFLFAVRSQKHKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPLGSQPAGRGPKGTVVPATPTKPQRTSPTNPHQHIAGRGPAQTPPAKLSASNTLDPVLPASLGSSTRTPRPAASQPSQKITATKIPKSLPTKPSAPSTSIVPVKSPHPTQKTAAPSFTKSAPPTQKHVPTVPAKVSRPAEKPIQRNQGMPRPPLPSTQPLPPTTSSSKKPIPTLAQTEAKVTNHASKPASARTSTHKPPPFTALSSSPAPTPGSTRTTRPPATMVPPTSGTSAPRTAPAVPTPGSAPTGNKKPTGSEASKKAGPESSPRKPVPLRPGKAARDVPLSDLTTRPSPRQSQPSQQTTSALVLAPAQFLSSSPRPTSSGYSFFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGEQGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPVGPPGEMGPKGPPGAAGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGELGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGRPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGADGLPGRDGQVGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGVAFQTWMDTSGALRPEGYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIVSVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_015117010.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015117010.2

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGGGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGTCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAAACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGCGA CATGGGCTTG CCTGGGCTGT CCGGGAATCC AGGACCTCCG 
GGACGAAAGG GACACAAGGG CTATCCTGGA CCGGCAGGGC ACCCCGGAGA ACAGGGGCAG 
CCAGGACCTG AGGGCAGCCC AGGGGCCAAA GGTTACCCTG GCAGGCAGGG GTTACCTGGA 
CCGGTAGGAG ATCCCGGCCC CAAAGGCAGC AGGGGCTACA TTGGGCTCCC AGGGCTCTTC 
GGCCTGCCAG GGTCTGATGG AGAGCGAGGC CTTCCTGGTG TTCCTGGCAA GAGGGGCAAG 
ATGGGTATGC CGGGGTTTCC TGGAGTCTTT GGGGAAAGAG GCCCTCCCGG ACTGGATGGA 
AACCCTGGAG AACTGGGCCT GCCAGGCCCC CCTGGAGTCC CCGGCCTCAT TGGTGACTTG 
GGAGTGTTGG GTCCGATTGG CTACCCAGGA CCCAAGGGCA TGAAGGGACT GATGGGCAGC 
GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTTCCAGG TGTGTCGGGA 
GATCCCGGAT TCCAAGGAGA CAAGGGGAGC CAGGGGTTGC CAGGGTTCCC CGGCGCACGG 
GGGAAGCCAG GGCCTCTGGG CAAAGTTGGA GACAAAGGAT CCATTGGGTT TCCCGGGCCC 
CCTGGACCCG AGGGATTCCC AGGAGACATC GGCCCCCCTG GTGACAACGG CCCAGAAGGC 
ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCGGGACCCC GTGGGCAGCT GGGGCCCGAG 
GGAGATGAGG GACCCATGGG GCCACCAGGG GCCCCTGGCT TGGAGGGTCA GCCTGGCAGG 
AAGGGCTTTC CTGGGAGGCC CGGCCTGGAT GGCGTGAAGG GGGAACCGGG AGATCCTGGT 
CGGCCAGGGC CTGTGGGAGA GCAGGGATTT ATGGGATTCA TTGGTCTGGT CGGGGAGCCA 
GGAATCGTGG GAGAAAAGGG TGATCGTGGC ATGATGGGAC CCCCAGGCGT GCCTGGACCC 
AAGGGGTCGA TGGGTCATCC TGGAATGCCA GGTGGTATGG GGACCCCTGG AGAACCTGGA 
CCCCAGGGTC CTCCAGGATC TCGAGGCCCA CCAGGCATGA GGGGAGCGAA GGGACGTCGG 
GGCCCCCGAG GACCGGACGG ACCAGCTGGG GAGCAAGGGT CCAGGGGCCT GAAGGGCCCT 
CCAGGACCCC AGGGCAGACC GGGCCGGCCT GGACAGCAGG GTGTGGCTGG TGAGCAAGGC 
CACTTGGGCT CGCGAGGCTT TCCCGGCATC CCGGGTCCCT CAGGCCCCCC AGGCACCAAG 
GGCCTCCCAG GAGAACCGGG CCCTCAGGGA CCCCAGGGGC CAGTTGGGCC TCCAGGAGAG 
ATGGGACCTA AGGGGCCGCC GGGTGCAGCG GGAGAACCGG GCCTTCCTGG GGAAGCTGGG 
ATGAAGGGTG ACCTTGGACC CCTGGGCACC CCTGGGGAGC AGGGCCTCAT TGGGCAACGG 
GGAGAGCCAG GCCTCGAGGG TGACAGTGGC CCCATGGGAC CTGATGGGCT GAAGGGGGAC 
AGGGGAGACC CAGGGCCTGA TGGAGAACAT GGCGAGAAAG GCCAGGAAGG GCTGATGGGT 
GAGGACGGGC CCCCCGGCCC CCCTGGTGTC ACTGGTGTCC GGGGTCCTGA AGGAAAATCA 
GGGAAGCAAG GTGAGAAGGG CCGCACTGGA GCCAAGGGCG CCAAGGGCTA CCAAGGACAG 
CTGGGTGAGA TGGGCGTCCC TGGAGACCCC GGACCCCCTG GCACTCCAGG CCCTAAAGGG 
TCCCGGGGCA GCCTGGGACC AACGGGTGCT CCTGGACGCA TGGGGGCCCA AGGAGAACTG 
GGACTGGCTG GTTATGATGG ACACAAAGGC ATTGTGGGAC CCCTCGGACC TCCTGGACCA 
AAAGGCGAAA AGGGGGAGCA GGGCGAGGAC GGCAAGGCCG AGGGGCCCCC TGGGCCACCT 
GGAGATCGGG GCCCTGTGGG TGATCGAGGA GACCGCGGGG AACCCGGGGA CCCTGGGTAC 
CCTGGACAGG AGGGTGTGCA AGGCCTCCGT GGAAAGCCAG GCCAGCAGGG CCAACCCGGG 
CATCCGGGAC CCCGGGGGCG GCCGGGACCC AAAGGATCTA AAGGCGCAGA GGGACCAAAG 
GGAAAGCAAG GCAAGGCGGG GGCCCCAGGC CGGAGGGGGG TCCAGGGCCT GCAGGGGCTG 
CCAGGGCCCC GGGGCGTGGT GGGGAGACAG GGCCTCGAGG GCATCGCTGG AGCAGATGGG 
CTTCCTGGCA GGGACGGGCA AGTGGGACAG CAGGGGGAGC AGGGAGACGA TGGGGACCCT 
GGCCCCATGG GCCCTGCTGG GAAGAGAGGA AATCCAGGTG TGGCCGGCTT ACCTGGAGCA 
CAGGGACCCC CAGGATTCAA GGGTGAGAGT GGGTTACCCG GACAGCTGGG TCCCCCTGGC 
AAACGAGGGA CGGAGGGCAG AACAGGGCTC CCTGGAAACC AGGGGGAGCC TGGGTCCAAA 
GGCCAGCCGG GCGACTCTGG TGAGATGGGC TTCCCAGGAA TGGCAGGTCT CTTCGGACCT 
AAGGGCCCCC CTGGAGACAT CGGCTTCAAA GGCATCCAGG GTCCTCGGGG GCCACCCGGT 
TTGATGGGAA AGGAAGGCAT CATCGGGCCC CTCGGAATCC TGGGACCTTC GGGACTCCCG 
GGTCCGAAGG GTGACAAAGG CAGCCGTGGG GACTGGGGAT TGCAAGGTCC GAGGGGTCCT 
CCTGGCCCCA GAGGGCGGCC CGGCCCCCCG GGTCCTCCAG GGGGTCCTAT CCAATTGCAA 
CAAGATGATC TTGGGGTAGC TTTCCAGACG TGGATGGACA CCAGTGGAGC ACTCAGACCA 
GAGGGTTACA GCTATCCGGA CCGGCTGGTG CTTGACCAGG GAGGAGAGAT CTTTAAAACC 
TTACACTACC TCAGCAACCT CATCCAGAGC ATTAAGACGC CCCTGGGCAC CAAAGAGAAC 
CCCGCCCGGG TCTGCAGGGA CCTCATGGAC TGTGAGCAGA AGATGGTGGA TGGTACCTAC 
TGGGTGGATC CAAACCTTGG CTGCTCATCC GACACCATTG AAGTCTCCTG CAACTTCACG 
CATGGTGGAC AGACGTGTCT CAAACCCATC ACGGCCTCCA AGGTCGAGTT TGCCATCAGC 
CGGGTCCAGA TGAATTTCCT GCACCTGCTA AGCTCTGAGG TGACCCAGCA TATCACCATC 
CACTGCCTTA ACATGACTGT GTGGCAGGAG GGCACTGGGC AGACCCCAGC CAAGCAGGCC 
GTGCGCTTCC GGGCCTGGAA CGGACAGATT TTTGAAGCTG GGGGTCAGTT CCGGCCCGAG 
GTGTCCATGG ATGGCTGCAA GGTCCAAGAC GGCCGCTGGC ATCAGACACT CTTCACCTTC 
CGGACCCAAG ACCCCCAACA GCTGCCCATC GTCAGTGTGG ACAACCTCCC TCCTGCCTCA 
TCAGGGAAGC AGTACCGCCT GGAAGTTGGA CCTGCGTGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_028834927.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028690760.1 Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X5, mRNA.

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CloneID	OMb71342
Clone ID Related Accession (Same CDS sequence)	XM_028834927.1
Accession Version	XM_028834927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	collagen alpha-1(XXVII) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGGGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGTCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAAACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGACA GAAAGGGGAC CCAGGGCTCT CACCAGGAAA GGCCCACGAT 
GGGGCAAAGG GCGACATGGG CTTGCCTGGG CTGTCCGGGA ATCCAGGACC TCCGGGACGA 
AAGGGACACA AGGGCTATCC TGGACCGGCA GGGCACCCCG GAGAACAGGG GCAGCCAGGA 
CCTGAGGGCA GCCCAGGGGC CAAAGGTTAC CCTGGCAGGC AGGGGTTACC TGGACCGGTA 
GGAGATCCCG GCCCCAAAGG CAGCAGGGGC TACATTGGGC TCCCAGGGCT CTTCGGCCTG 
CCAGGGTCTG ATGGAGAGCG AGGCCTTCCT GGTGTTCCTG GCAAGAGGGG CAAGATGGGT 
ATGCCGGGGT TTCCTGGAGT CTTTGGGGAA AGAGGCCCTC CCGGACTGGA TGGAAACCCT 
GGAGAACTGG GCCTGCCAGG CCCCCCTGGA GTCCCCGGCC TCATTGGTGA CTTGGGAGTG 
TTGGGTCCGA TTGGCTACCC AGGACCCAAG GGCATGAAGG GACTGATGGG CAGCGTGGGG 
GAGCCCGGAC TGAAAGGTGA TAAGGGTGAA CAAGGGGTTC CAGGTGTGTC GGGAGATCCC 
GGATTCCAAG GAGACAAGGG GAGCCAGGGG TTGCCAGGGT TCCCCGGCGC ACGGGGGAAG 
CCAGGGCCTC TGGGCAAAGT TGGAGACAAA GGATCCATTG GGTTTCCCGG GCCCCCTGGA 
CCCGAGGGAT TCCCAGGAGA CATCGGCCCC CCTGGTGACA ACGGCCCAGA AGGCATGAAG 
GGTAAGCCTG GAGCCCGAGG CCTGCCGGGA CCCCGTGGGC AGCTGGGGCC CGAGGGAGAT 
GAGGGACCCA TGGGGCCACC AGGGGCCCCT GGCTTGGAGG GTCAGCCTGG CAGGAAGGGC 
TTTCCTGGGA GGCCCGGCCT GGATGGCGTG AAGGGGGAAC CGGGAGATCC TGGTCGGCCA 
GGGCCTGTGG GAGAGCAGGG ATTTATGGGA TTCATTGGTC TGGTCGGGGA GCCAGGAATC 
GTGGGAGAAA AGGGTGATCG TGGCATGATG GGACCCCCAG GCGTGCCTGG ACCCAAGGGG 
TCGATGGGTC ATCCTGGAAT GCCAGGTGGT ATGGGGACCC CTGGAGAACC TGGACCCCAG 
GGTCCTCCAG GATCTCGAGG CCCACCAGGC ATGAGGGGAG CGAAGGGACG TCGGGGCCCC 
CGAGGACCGG ACGGACCAGC TGGGGAGCAA GGGTCCAGGG GCCTGAAGGG CCCTCCAGGA 
CCCCAGGGCA GACCGGGCCG GCCTGGACAG CAGGGTGTGG CTGGTGAGCA AGGCCACTTG 
GGCTCGCGAG GCTTTCCCGG CATCCCGGGT CCCTCAGGCC CCCCAGGCAC CAAGGGCCTC 
CCAGGAGAAC CGGGCCCTCA GGGACCCCAG GGGCCAGTTG GGCCTCCAGG AGAGATGGGA 
CCTAAGGGGC CGCCGGGTGC AGCGGGAGAA CCGGGCCTTC CTGGGGAAGC TGGGATGAAG 
GGTGACCTTG GACCCCTGGG CACCCCTGGG GAGCAGGGCC TCATTGGGCA ACGGGGAGAG 
CCAGGCCTCG AGGGTGACAG TGGCCCCATG GGACCTGATG GGCTGAAGGG GGACAGGGGA 
GACCCAGGGC CTGATGGAGA ACATGGCGAG AAAGGCCAGG AAGGGCTGAT GGGTGAGGAC 
GGGCCCCCCG GCCCCCCTGG TGTCACTGGT GTCCGGGGTC CTGAAGGAAA ATCAGGGAAG 
CAAGGTGAGA AGGGCCGCAC TGGAGCCAAG GGCGCCAAGG GCTACCAAGG ACAGCTGGGT 
GAGATGGGCG TCCCTGGAGA CCCCGGACCC CCTGGCACTC CAGGCCCTAA AGGGTCCCGG 
GGCAGCCTGG GACCAACGGG TGCTCCTGGA CGCATGGGGG CCCAAGGAGA ACTGGGACTG 
GCTGGTTATG ATGGACACAA AGGCATTGTG GGACCCCTCG GACCTCCTGG ACCAAAAGGC 
GAAAAGGGGG AGCAGGGCGA GGACGGCAAG GCCGAGGGGC CCCCTGGGCC ACCTGGAGAT 
CGGGGCCCTG TGGGTGATCG AGGAGACCGC GGGGAACCCG GGGACCCTGG GTACCCTGGA 
CAGGAGGGTG TGCAAGGCCT CCGTGGAAAG CCAGGCCAGC AGGGCCAACC CGGGCATCCG 
GGACCCCGGG GGCGGCCGGG ACCCAAAGGA TCTAAAGGCG CAGAGGGACC AAAGGGAAAG 
CAAGGCAAGG CGGGGGCCCC AGGCCGGAGG GGGGTCCAGG GCCTGCAGGG GCTGCCAGGG 
CCCCGGGGCG TGGTGGGGAG ACAGGGCCTC GAGGGCATCG CTGGAGCAGA TGGGCTTCCT 
GGCAGGGACG GGCAAGTGGG ACAGCAGGGG GAGCAGGGAG ACGATGGGGA CCCTGGCCCC 
ATGGGCCCTG CTGGGAAGAG AGGAAATCCA GGTGTGGCCG GCTTACCTGG AGCACAGGGA 
CCCCCAGGAT TCAAGGGTGA GAGTGGGTTA CCCGGACAGC TGGGTCCCCC TGGCAAACGA 
GGGACGGAGG GCAGAACAGG GCTCCCTGGA AACCAGGGGG AGCCTGGGTC CAAAGGCCAG 
CCGGGCGACT CTGGTGAGAT GGGCTTCCCA GGAATGGCAG GTCTCTTCGG ACCTAAGGGC 
CCCCCTGGAG ACATCGGCTT CAAAGGCATC CAGGGTCCTC GGGGGCCACC CGGTTTGATG 
GGAAAGGAAG GCATCATCGG GCCCCTCGGA ATCCTGGGAC CTTCGGGACT CCCGGGTCCG 
AAGGGTGACA AAGGCAGCCG TGGGGACTGG GGATTGCAAG GTCCGAGGGG TCCTCCTGGC 
CCCAGAGGGC GGCCCGGCCC CCCGGGTCCT CCAGGGGGTC CTATCCAATT GGGTTACAGC 
TATCCGGACC GGCTGGTGCT TGACCAGGGA GGAGAGATCT TTAAAACCTT ACACTACCTC 
AGCAACCTCA TCCAGAGCAT TAAGACGCCC CTGGGCACCA AAGAGAACCC CGCCCGGGTC 
TGCAGGGACC TCATGGACTG TGAGCAGAAG ATGGTGGATG GTACCTACTG GGTGGATCCA 
AACCTTGGCT GCTCATCCGA CACCATTGAA GTCTCCTGCA ACTTCACGCA TGGTGGACAG 
ACGTGTCTCA AACCCATCAC GGCCTCCAAG GTCGAGTTTG CCATCAGCCG GGTCCAGATG 
AATTTCCTGC ACCTGCTAAG CTCTGAGGTG ACCCAGCATA TCACCATCCA CTGCCTTAAC 
ATGACTGTGT GGCAGGAGGG CACTGGGCAG ACCCCAGCCA AGCAGGCCGT GCGCTTCCGG 
GCCTGGAACG GACAGATTTT TGAAGCTGGG GGTCAGTTCC GGCCCGAGGT GTCCATGGAT 
GGCTGCAAGG TCCAAGACGG CCGCTGGCAT CAGACACTCT TCACCTTCCG GACCCAAGAC 
CCCCAACAGC TGCCCATCGT CAGTGTGGAC AACCTCCCTC CTGCCTCATC AGGGAAGCAG 
TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028690760.1
CDS	460..5955
Translation	MGAGSARGARGTATAAAAHGGGFLFSWILVSFACHLASTQGAPEDVDVLQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGSVIPASLGTELALVLSLCSHRVNHAFLFAVRSQKHKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPLGSQPAGRGPKGTVVPATPTKPQRTSPTNPHQHIAGRGPAQTPPAKLSASNTLDPVLPASLGSSTRTPRPAASQPSQKITATKIPKSLPTKPSAPSTSIVPVKSPHPTQKTAAPSFTKSAPPTQKHVPTVPAKVSRPAEKPIQRNQGMPRPPLPSTQPLPPTTSSSKKPIPTLAQTEAKVTNHASKPASARTSTHKPPPFTALSSSPAPTPGSTRTTRPPATMVPPTSGTSAPRTAPAVPTPGSAPTGNKKPTGSEASKKAGPESSPRKPVPLRPGKAARDVPLSDLTTRPSPRQSQPSQQTTSALVLAPAQFLSSSPRPTSSGYSFFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGEQGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPVGPPGEMGPKGPPGAAGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGELGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGRPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGADGLPGRDGQVGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLGYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIVSVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_028834927.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028834927.1

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGGGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGTCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAAACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGACA GAAAGGGGAC CCAGGGCTCT CACCAGGAAA GGCCCACGAT 
GGGGCAAAGG GCGACATGGG CTTGCCTGGG CTGTCCGGGA ATCCAGGACC TCCGGGACGA 
AAGGGACACA AGGGCTATCC TGGACCGGCA GGGCACCCCG GAGAACAGGG GCAGCCAGGA 
CCTGAGGGCA GCCCAGGGGC CAAAGGTTAC CCTGGCAGGC AGGGGTTACC TGGACCGGTA 
GGAGATCCCG GCCCCAAAGG CAGCAGGGGC TACATTGGGC TCCCAGGGCT CTTCGGCCTG 
CCAGGGTCTG ATGGAGAGCG AGGCCTTCCT GGTGTTCCTG GCAAGAGGGG CAAGATGGGT 
ATGCCGGGGT TTCCTGGAGT CTTTGGGGAA AGAGGCCCTC CCGGACTGGA TGGAAACCCT 
GGAGAACTGG GCCTGCCAGG CCCCCCTGGA GTCCCCGGCC TCATTGGTGA CTTGGGAGTG 
TTGGGTCCGA TTGGCTACCC AGGACCCAAG GGCATGAAGG GACTGATGGG CAGCGTGGGG 
GAGCCCGGAC TGAAAGGTGA TAAGGGTGAA CAAGGGGTTC CAGGTGTGTC GGGAGATCCC 
GGATTCCAAG GAGACAAGGG GAGCCAGGGG TTGCCAGGGT TCCCCGGCGC ACGGGGGAAG 
CCAGGGCCTC TGGGCAAAGT TGGAGACAAA GGATCCATTG GGTTTCCCGG GCCCCCTGGA 
CCCGAGGGAT TCCCAGGAGA CATCGGCCCC CCTGGTGACA ACGGCCCAGA AGGCATGAAG 
GGTAAGCCTG GAGCCCGAGG CCTGCCGGGA CCCCGTGGGC AGCTGGGGCC CGAGGGAGAT 
GAGGGACCCA TGGGGCCACC AGGGGCCCCT GGCTTGGAGG GTCAGCCTGG CAGGAAGGGC 
TTTCCTGGGA GGCCCGGCCT GGATGGCGTG AAGGGGGAAC CGGGAGATCC TGGTCGGCCA 
GGGCCTGTGG GAGAGCAGGG ATTTATGGGA TTCATTGGTC TGGTCGGGGA GCCAGGAATC 
GTGGGAGAAA AGGGTGATCG TGGCATGATG GGACCCCCAG GCGTGCCTGG ACCCAAGGGG 
TCGATGGGTC ATCCTGGAAT GCCAGGTGGT ATGGGGACCC CTGGAGAACC TGGACCCCAG 
GGTCCTCCAG GATCTCGAGG CCCACCAGGC ATGAGGGGAG CGAAGGGACG TCGGGGCCCC 
CGAGGACCGG ACGGACCAGC TGGGGAGCAA GGGTCCAGGG GCCTGAAGGG CCCTCCAGGA 
CCCCAGGGCA GACCGGGCCG GCCTGGACAG CAGGGTGTGG CTGGTGAGCA AGGCCACTTG 
GGCTCGCGAG GCTTTCCCGG CATCCCGGGT CCCTCAGGCC CCCCAGGCAC CAAGGGCCTC 
CCAGGAGAAC CGGGCCCTCA GGGACCCCAG GGGCCAGTTG GGCCTCCAGG AGAGATGGGA 
CCTAAGGGGC CGCCGGGTGC AGCGGGAGAA CCGGGCCTTC CTGGGGAAGC TGGGATGAAG 
GGTGACCTTG GACCCCTGGG CACCCCTGGG GAGCAGGGCC TCATTGGGCA ACGGGGAGAG 
CCAGGCCTCG AGGGTGACAG TGGCCCCATG GGACCTGATG GGCTGAAGGG GGACAGGGGA 
GACCCAGGGC CTGATGGAGA ACATGGCGAG AAAGGCCAGG AAGGGCTGAT GGGTGAGGAC 
GGGCCCCCCG GCCCCCCTGG TGTCACTGGT GTCCGGGGTC CTGAAGGAAA ATCAGGGAAG 
CAAGGTGAGA AGGGCCGCAC TGGAGCCAAG GGCGCCAAGG GCTACCAAGG ACAGCTGGGT 
GAGATGGGCG TCCCTGGAGA CCCCGGACCC CCTGGCACTC CAGGCCCTAA AGGGTCCCGG 
GGCAGCCTGG GACCAACGGG TGCTCCTGGA CGCATGGGGG CCCAAGGAGA ACTGGGACTG 
GCTGGTTATG ATGGACACAA AGGCATTGTG GGACCCCTCG GACCTCCTGG ACCAAAAGGC 
GAAAAGGGGG AGCAGGGCGA GGACGGCAAG GCCGAGGGGC CCCCTGGGCC ACCTGGAGAT 
CGGGGCCCTG TGGGTGATCG AGGAGACCGC GGGGAACCCG GGGACCCTGG GTACCCTGGA 
CAGGAGGGTG TGCAAGGCCT CCGTGGAAAG CCAGGCCAGC AGGGCCAACC CGGGCATCCG 
GGACCCCGGG GGCGGCCGGG ACCCAAAGGA TCTAAAGGCG CAGAGGGACC AAAGGGAAAG 
CAAGGCAAGG CGGGGGCCCC AGGCCGGAGG GGGGTCCAGG GCCTGCAGGG GCTGCCAGGG 
CCCCGGGGCG TGGTGGGGAG ACAGGGCCTC GAGGGCATCG CTGGAGCAGA TGGGCTTCCT 
GGCAGGGACG GGCAAGTGGG ACAGCAGGGG GAGCAGGGAG ACGATGGGGA CCCTGGCCCC 
ATGGGCCCTG CTGGGAAGAG AGGAAATCCA GGTGTGGCCG GCTTACCTGG AGCACAGGGA 
CCCCCAGGAT TCAAGGGTGA GAGTGGGTTA CCCGGACAGC TGGGTCCCCC TGGCAAACGA 
GGGACGGAGG GCAGAACAGG GCTCCCTGGA AACCAGGGGG AGCCTGGGTC CAAAGGCCAG 
CCGGGCGACT CTGGTGAGAT GGGCTTCCCA GGAATGGCAG GTCTCTTCGG ACCTAAGGGC 
CCCCCTGGAG ACATCGGCTT CAAAGGCATC CAGGGTCCTC GGGGGCCACC CGGTTTGATG 
GGAAAGGAAG GCATCATCGG GCCCCTCGGA ATCCTGGGAC CTTCGGGACT CCCGGGTCCG 
AAGGGTGACA AAGGCAGCCG TGGGGACTGG GGATTGCAAG GTCCGAGGGG TCCTCCTGGC 
CCCAGAGGGC GGCCCGGCCC CCCGGGTCCT CCAGGGGGTC CTATCCAATT GGGTTACAGC 
TATCCGGACC GGCTGGTGCT TGACCAGGGA GGAGAGATCT TTAAAACCTT ACACTACCTC 
AGCAACCTCA TCCAGAGCAT TAAGACGCCC CTGGGCACCA AAGAGAACCC CGCCCGGGTC 
TGCAGGGACC TCATGGACTG TGAGCAGAAG ATGGTGGATG GTACCTACTG GGTGGATCCA 
AACCTTGGCT GCTCATCCGA CACCATTGAA GTCTCCTGCA ACTTCACGCA TGGTGGACAG 
ACGTGTCTCA AACCCATCAC GGCCTCCAAG GTCGAGTTTG CCATCAGCCG GGTCCAGATG 
AATTTCCTGC ACCTGCTAAG CTCTGAGGTG ACCCAGCATA TCACCATCCA CTGCCTTAAC 
ATGACTGTGT GGCAGGAGGG CACTGGGCAG ACCCCAGCCA AGCAGGCCGT GCGCTTCCGG 
GCCTGGAACG GACAGATTTT TGAAGCTGGG GGTCAGTTCC GGCCCGAGGT GTCCATGGAT 
GGCTGCAAGG TCCAAGACGG CCGCTGGCAT CAGACACTCT TCACCTTCCG GACCCAAGAC 
CCCCAACAGC TGCCCATCGT CAGTGTGGAC AACCTCCCTC CTGCCTCATC AGGGAAGCAG 
TACCGCCTGG AAGTTGGACC TGCGTGCTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_028834928.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028690761.1 Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X6, mRNA.

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CloneID	OMb71343
Clone ID Related Accession (Same CDS sequence)	XM_028834928.1
Accession Version	XM_028834928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	collagen alpha-1(XXVII) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGGGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGTCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAAACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGACA GAAAGGGGAC CCAGGGCTCT CACCAGGAAA GGCCCACGAT 
GGGGCAAAGG GCGACATGGG CTTGCCTGGG CTGTCCGGGA ATCCAGGACC TCCGGGACGA 
AAGGGACACA AGGGCTATCC TGGACCGGCA GGGCACCCCG GAGAACAGGG GCAGCCAGGA 
CCTGAGGGCA GCCCAGGGGC CAAAGGTTAC CCTGGCAGGC AGGGGTTACC TGGACCGGTA 
GGAGATCCCG GCCCCAAAGG CAGCAGGGGC TACATTGGGC TCCCAGGGCT CTTCGGCCTG 
CCAGGGTCTG ATGGAGAGCG AGGCCTTCCT GGTGTTCCTG GCAAGAGGGG CAAGATGGGT 
ATGCCGGGGT TTCCTGGAGT CTTTGGGGAA AGAGGCCCTC CCGGACTGGA TGGAAACCCT 
GGAGAACTGG GCCTGCCAGG CCCCCCTGGA GTCCCCGGCC TCATTGGTGA CTTGGGAGTG 
TTGGGTCCGA TTGGCTACCC AGGACCCAAG GGCATGAAGG GACTGATGGG CAGCGTGGGG 
GAGCCCGGAC TGAAAGGTGA TAAGGGTGAA CAAGGGGTTC CAGGTGTGTC GGGAGATCCC 
GGATTCCAAG GAGACAAGGG GAGCCAGGGG TTGCCAGGGT TCCCCGGCGC ACGGGGGAAG 
CCAGGGCCTC TGGGCAAAGT TGGAGACAAA GGATCCATTG GGTTTCCCGG GCCCCCTGGA 
CCCGAGGGAT TCCCAGGAGA CATCGGCCCC CCTGGTGACA ACGGCCCAGA AGGCATGAAG 
GGTAAGCCTG GAGCCCGAGG CCTGCCGGGA CCCCGTGGGC AGCTGGGGCC CGAGGGAGAT 
GAGGGACCCA TGGGGCCACC AGGGGCCCCT GGCTTGGAGG GTCAGCCTGG CAGGAAGGGC 
TTTCCTGGGA GGCCCGGCCT GGATGGCGTG AAGGGGGAAC CGGGAGATCC TGGTCGGCCA 
GGGCCTGTGG GAGAGCAGGG ATTTATGGGA TTCATTGGTC TGGTCGGGGA GCCAGGAATC 
GTGGGAGAAA AGGGTGATCG TGGCATGATG GGACCCCCAG GCGTGCCTGG ACCCAAGGGG 
TCGATGGGTC ATCCTGGAAT GCCAGGTGGT ATGGGGACCC CTGGAGAACC TGGACCCCAG 
GGTCCTCCAG GATCTCGAGG CCCACCAGGC ATGAGGGGAG CGAAGGGACG TCGGGGCCCC 
CGAGGACCGG ACGGACCAGC TGGGGAGCAA GGGTCCAGGG GCCTGAAGGG CCCTCCAGGA 
CCCCAGGGCA GACCGGGCCG GCCTGGACAG CAGGGTGTGG CTGGTGAGCA AGGCCACTTG 
GGCTCGCGAG GCTTTCCCGG CATCCCGGGT CCCTCAGGCC CCCCAGGCAC CAAGGGCCTC 
CCAGGAGAAC CGGGCCCTCA GGGACCCCAG GGGCCAGTTG GGCCTCCAGG AGAGATGGGA 
CCTAAGGGGC CGCCGGGTGC AGCGGGAGAA CCGGGCCTTC CTGGGGAAGC TGGGATGAAG 
GGTGACCTTG GACCCCTGGG CACCCCTGGG GAGCAGGGCC TCATTGGGCA ACGGGGAGAG 
CCAGGCCTCG AGGGTGACAG TGGCCCCATG GGACCTGATG GGCTGAAGGG GGACAGGGGA 
GACCCAGGGC CTGATGGAGA ACATGGCGAG AAAGGCCAGG AAGGGCTGAT GGGTGAGGAC 
GGGCCCCCCG GCCCCCCTGG TGTCACTGGT GTCCGGGGTC CTGAAGGAAA ATCAGGGAAG 
CAAGGTGAGA AGGGCCGCAC TGGAGCCAAG GGCGCCAAGG GCTACCAAGG ACAGCTGGGT 
GAGATGGGCG TCCCTGGAGA CCCCGGACCC CCTGGCACTC CAGGCCCTAA AGGGTCCCGG 
GGCAGCCTGG GACCAACGGG TGCTCCTGGA CGCATGGGGG CCCAAGGAGA ACTGGGACTG 
GCTGGTTATG ATGGACACAA AGGCATTGTG GGACCCCTCG GACCTCCTGG ACCAAAAGGC 
GAAAAGGGGG AGCAGGGCGA GGACGGCAAG GCCGAGGGGC CCCCTGGGCC ACCTGGAGAT 
CGGGGCCCTG TGGGTGATCG AGGAGACCGC GGGGAACCCG GGGACCCTGG GTACCCTGGA 
CAGGAGGGTG TGCAAGGCCT CCGTGGAAAG CCAGGCCAGC AGGGCCAACC CGGGCATCCG 
GGACCCCGGG GGCGGCCGGG ACCCAAAGGA TCTAAAGGCG CAGAGGGACC AAAGGGAAAG 
CAAGGCAAGG CGGGGGCCCC AGGCCGGAGG GGGGTCCAGG GCCTGCAGGG GCTGCCAGGG 
CCCCGGGGCG TGGTGGGGAG ACAGGGCCTC GAGGGCATCG CTGGAGCAGA TGGGCTTCCT 
GGCAGGGACG GGCAAGTGGG ACAGCAGGGG GAGCAGGGAG ACGATGGGGA CCCTGGCCCC 
ATGGGCCCTG CTGGGAAGAG AGGAAATCCA GGTGTGGCCG GCTTACCTGG AGCACAGGGA 
CCCCCAGGAT TCAAGGGTGA GAGTGGGTTA CCCGGACAGC TGGGTCCCCC TGGCAAACGA 
GGGACGGAGG GCAGAACAGG GCTCCCTGGA AACCAGGGGG AGCCTGGGTC CAAAGGCCAG 
CCGGGCGACT CTGGTGAGAT GGGCTTCCCA GGAATGGCAG GTCTCTTCGG ACCTAAGGGC 
CCCCCTGGAG ACATCGGCTT CAAAGGCATC CAGGGTCCTC GGGGGCCACC CGGTTTGATG 
GGAAAGGAAG GCATCATCGG GCCCCTCGGA ATCCTGGGAC CTTCGGGACT CCCGGGTCCG 
AAGGGTGACA AAGGCAGCCG TGGGGACTGG GGATTGCAAG GGTCCTCCTG GCCCCAGAGG 
GCGGCCCGGC CCCCCGGGTC CTCCAGGGGG TCCTATCCAA TTGGGTTACA GCTATCCGGA 
CCGGCTGGTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028690761.1
CDS	463..5337
Translation	MGAGSARGARGTATAAAAHGGGFLFSWILVSFACHLASTQGAPEDVDVLQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGSVIPASLGTELALVLSLCSHRVNHAFLFAVRSQKHKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPLGSQPAGRGPKGTVVPATPTKPQRTSPTNPHQHIAGRGPAQTPPAKLSASNTLDPVLPASLGSSTRTPRPAASQPSQKITATKIPKSLPTKPSAPSTSIVPVKSPHPTQKTAAPSFTKSAPPTQKHVPTVPAKVSRPAEKPIQRNQGMPRPPLPSTQPLPPTTSSSKKPIPTLAQTEAKVTNHASKPASARTSTHKPPPFTALSSSPAPTPGSTRTTRPPATMVPPTSGTSAPRTAPAVPTPGSAPTGNKKPTGSEASKKAGPESSPRKPVPLRPGKAARDVPLSDLTTRPSPRQSQPSQQTTSALVLAPAQFLSSSPRPTSSGYSFFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGEQGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPVGPPGEMGPKGPPGAAGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGELGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGRPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGADGLPGRDGQVGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPLGILGPSGLPGPKGDKGSRGDWGLQGSSWPQRAARPPGSSRGSYPIGLQLSGPAGA

Target ORF information:

RefSeq Version	XM_028834928.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta collagen type XXVII alpha 1 chain (COL27A1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028834928.1

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCTGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGGGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGTCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAAACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGACA GAAAGGGGAC CCAGGGCTCT CACCAGGAAA GGCCCACGAT 
GGGGCAAAGG GCGACATGGG CTTGCCTGGG CTGTCCGGGA ATCCAGGACC TCCGGGACGA 
AAGGGACACA AGGGCTATCC TGGACCGGCA GGGCACCCCG GAGAACAGGG GCAGCCAGGA 
CCTGAGGGCA GCCCAGGGGC CAAAGGTTAC CCTGGCAGGC AGGGGTTACC TGGACCGGTA 
GGAGATCCCG GCCCCAAAGG CAGCAGGGGC TACATTGGGC TCCCAGGGCT CTTCGGCCTG 
CCAGGGTCTG ATGGAGAGCG AGGCCTTCCT GGTGTTCCTG GCAAGAGGGG CAAGATGGGT 
ATGCCGGGGT TTCCTGGAGT CTTTGGGGAA AGAGGCCCTC CCGGACTGGA TGGAAACCCT 
GGAGAACTGG GCCTGCCAGG CCCCCCTGGA GTCCCCGGCC TCATTGGTGA CTTGGGAGTG 
TTGGGTCCGA TTGGCTACCC AGGACCCAAG GGCATGAAGG GACTGATGGG CAGCGTGGGG 
GAGCCCGGAC TGAAAGGTGA TAAGGGTGAA CAAGGGGTTC CAGGTGTGTC GGGAGATCCC 
GGATTCCAAG GAGACAAGGG GAGCCAGGGG TTGCCAGGGT TCCCCGGCGC ACGGGGGAAG 
CCAGGGCCTC TGGGCAAAGT TGGAGACAAA GGATCCATTG GGTTTCCCGG GCCCCCTGGA 
CCCGAGGGAT TCCCAGGAGA CATCGGCCCC CCTGGTGACA ACGGCCCAGA AGGCATGAAG 
GGTAAGCCTG GAGCCCGAGG CCTGCCGGGA CCCCGTGGGC AGCTGGGGCC CGAGGGAGAT 
GAGGGACCCA TGGGGCCACC AGGGGCCCCT GGCTTGGAGG GTCAGCCTGG CAGGAAGGGC 
TTTCCTGGGA GGCCCGGCCT GGATGGCGTG AAGGGGGAAC CGGGAGATCC TGGTCGGCCA 
GGGCCTGTGG GAGAGCAGGG ATTTATGGGA TTCATTGGTC TGGTCGGGGA GCCAGGAATC 
GTGGGAGAAA AGGGTGATCG TGGCATGATG GGACCCCCAG GCGTGCCTGG ACCCAAGGGG 
TCGATGGGTC ATCCTGGAAT GCCAGGTGGT ATGGGGACCC CTGGAGAACC TGGACCCCAG 
GGTCCTCCAG GATCTCGAGG CCCACCAGGC ATGAGGGGAG CGAAGGGACG TCGGGGCCCC 
CGAGGACCGG ACGGACCAGC TGGGGAGCAA GGGTCCAGGG GCCTGAAGGG CCCTCCAGGA 
CCCCAGGGCA GACCGGGCCG GCCTGGACAG CAGGGTGTGG CTGGTGAGCA AGGCCACTTG 
GGCTCGCGAG GCTTTCCCGG CATCCCGGGT CCCTCAGGCC CCCCAGGCAC CAAGGGCCTC 
CCAGGAGAAC CGGGCCCTCA GGGACCCCAG GGGCCAGTTG GGCCTCCAGG AGAGATGGGA 
CCTAAGGGGC CGCCGGGTGC AGCGGGAGAA CCGGGCCTTC CTGGGGAAGC TGGGATGAAG 
GGTGACCTTG GACCCCTGGG CACCCCTGGG GAGCAGGGCC TCATTGGGCA ACGGGGAGAG 
CCAGGCCTCG AGGGTGACAG TGGCCCCATG GGACCTGATG GGCTGAAGGG GGACAGGGGA 
GACCCAGGGC CTGATGGAGA ACATGGCGAG AAAGGCCAGG AAGGGCTGAT GGGTGAGGAC 
GGGCCCCCCG GCCCCCCTGG TGTCACTGGT GTCCGGGGTC CTGAAGGAAA ATCAGGGAAG 
CAAGGTGAGA AGGGCCGCAC TGGAGCCAAG GGCGCCAAGG GCTACCAAGG ACAGCTGGGT 
GAGATGGGCG TCCCTGGAGA CCCCGGACCC CCTGGCACTC CAGGCCCTAA AGGGTCCCGG 
GGCAGCCTGG GACCAACGGG TGCTCCTGGA CGCATGGGGG CCCAAGGAGA ACTGGGACTG 
GCTGGTTATG ATGGACACAA AGGCATTGTG GGACCCCTCG GACCTCCTGG ACCAAAAGGC 
GAAAAGGGGG AGCAGGGCGA GGACGGCAAG GCCGAGGGGC CCCCTGGGCC ACCTGGAGAT 
CGGGGCCCTG TGGGTGATCG AGGAGACCGC GGGGAACCCG GGGACCCTGG GTACCCTGGA 
CAGGAGGGTG TGCAAGGCCT CCGTGGAAAG CCAGGCCAGC AGGGCCAACC CGGGCATCCG 
GGACCCCGGG GGCGGCCGGG ACCCAAAGGA TCTAAAGGCG CAGAGGGACC AAAGGGAAAG 
CAAGGCAAGG CGGGGGCCCC AGGCCGGAGG GGGGTCCAGG GCCTGCAGGG GCTGCCAGGG 
CCCCGGGGCG TGGTGGGGAG ACAGGGCCTC GAGGGCATCG CTGGAGCAGA TGGGCTTCCT 
GGCAGGGACG GGCAAGTGGG ACAGCAGGGG GAGCAGGGAG ACGATGGGGA CCCTGGCCCC 
ATGGGCCCTG CTGGGAAGAG AGGAAATCCA GGTGTGGCCG GCTTACCTGG AGCACAGGGA 
CCCCCAGGAT TCAAGGGTGA GAGTGGGTTA CCCGGACAGC TGGGTCCCCC TGGCAAACGA 
GGGACGGAGG GCAGAACAGG GCTCCCTGGA AACCAGGGGG AGCCTGGGTC CAAAGGCCAG 
CCGGGCGACT CTGGTGAGAT GGGCTTCCCA GGAATGGCAG GTCTCTTCGG ACCTAAGGGC 
CCCCCTGGAG ACATCGGCTT CAAAGGCATC CAGGGTCCTC GGGGGCCACC CGGTTTGATG 
GGAAAGGAAG GCATCATCGG GCCCCTCGGA ATCCTGGGAC CTTCGGGACT CCCGGGTCCG 
AAGGGTGACA AAGGCAGCCG TGGGGACTGG GGATTGCAAG GGTCCTCCTG GCCCCAGAGG 
GCGGCCCGGC CCCCCGGGTC CTCCAGGGGG TCCTATCCAA TTGGGTTACA GCTATCCGGA 
CCGGCTGGTG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_015117009.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014972495.1 Macaca mulatta collagen, type XXVII, alpha 1 (COL27A1), transcript variant X1, mRNA.

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CloneID	OMb48770
Clone ID Related Accession (Same CDS sequence)	XM_015117009.1
Accession Version	XM_015117009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805511.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCAGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGTGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGCCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAGACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGACA GAAAGGGGAC CCAGGGCTCT CACCAGGAAA GGCCCACGAT 
GGGGCAAAGG GCGACATGGG CTTGCCTGGG CTGTCCGGGA ATCCAGGACC TCCGGGACGA 
AAGGGACACA AGGGCTATCC TGGACCGGCA GGGCACCCCG GAGAACAGGG GCAGCCAGGA 
CCTGAGGGCA GCCCAGGGGC CAAAGGTTAC CCTGGCAGGC AGGGGTTACC TGGACCGGTA 
GGAGATCCCG GCCCCAAAGG CAGCAGGGGC TACATTGGGC TCCCAGGGCT CTTCGGCCTG 
CCAGGGTCTG ATGGAGAGCG AGGCCTTCCT GGTGTTCCTG GCAAGAGGGG CAAGATGGGT 
ATGCCGGGGT TTCCTGGAGT CTTTGGGGAA AGAGGCCCTC CCGGACTGGA TGGAAACCCT 
GGAGAACTGG GCCTGCCAGG CCCCCCTGGA GTCCCCGGCC TCATTGGTGA CTTGGGAGTG 
TTGGGTCCGA TTGGCTACCC AGGACCCAAG GGCATGAAGG GACTGATGGG CAGCGTGGGG 
GAGCCCGGAC TGAAAGGTGA TAAGGGTGAA CAAGGGGTTC CAGGTGTGTC GGGAGATCCC 
GGATTCCAAG GAGACAAGGG GAGCCAGGGG TTGCCAGGGT TCCCCGGCGC ACGGGGGAAG 
CCAGGGCCTC TGGGCAAAGT TGGAGACAAA GGATCCATTG GGTTTCCCGG GCCCCCTGGA 
CCCGAGGGAT TCCCAGGAGA CATCGGCCCC CCTGGCGACA ACGGCCCAGA AGGCATGAAG 
GGTAAGCCTG GAGCCCGAGG CCTGCCGGGA CCCCGTGGGC AGCTGGGGCC CGAGGGAGAT 
GAGGGACCCA TGGGGCCACC AGGGGCCCCT GGCTTGGAGG GTCAGCCTGG CAGGAAGGGC 
TTTCCTGGGA GGCCCGGCCT GGATGGCGTG AAGGGGGAAC CGGGAGATCC TGGTCGGCCA 
GGGCCTGTGG GAGAGCAGGG ATTTATGGGA TTCATTGGTC TGGTCGGGGA GCCAGGAATC 
GTGGGAGAAA AGGGTGATCG TGGCATGATG GGACCCCCAG GCGTGCCTGG ACCCAAGGGG 
TCGATGGGTC ATCCTGGAAT GCCAGGTGGT ATGGGGACCC CTGGAGAACC TGGACCCCAG 
GGTCCTCCAG GATCTCGAGG CCCACCAGGC ATGAGGGGAG CGAAGGGACG TCGGGGCCCC 
CGAGGACCGG ACGGACCAGC TGGGGAGCAA GGGTCCAGGG GCCTGAAGGG CCCTCCAGGA 
CCCCAGGGCA GACCGGGCCG GCCTGGACAG CAGGGTGTGG CTGGTGAGCA AGGCCACTTG 
GGTTCGCGAG GCTTTCCCGG CATCCCGGGT CCCTCAGGCC CCCCAGGCAC CAAGGGCCTC 
CCAGGAGAAC CGGGCCCTCA GGGACCCCAG GGGCCAGTTG GGCCTCCAGG AGAGATGGGA 
CCTAAGGGGC CGCCGGGTGC AGCGGGAGAA CCGGGCCTTC CTGGGGAAGC TGGGATGAAG 
GGTGACCTTG GACCCCTGGG CACCCCTGGG GAGCAGGGCC TCATTGGGCA ACGGGGAGAG 
CCAGGCCTCG AGGGTGACAG TGGCCCCATG GGACCTGATG GGCTGAAGGG GGACAGGGGA 
GACCCAGGGC CTGATGGAGA ACATGGCGAG AAAGGCCAGG AAGGGCTGAT GGGTGAGGAC 
GGGCCCCCCG GCCCCCCTGG TGTCACTGGT GTCCGGGGTC CTGAAGGAAA ATCAGGGAAG 
CAAGGTGAGA AGGGCCGCAC TGGAGCCAAG GGCGCCAAGG GCTACCAAGG ACAGCTGGGT 
GAGATGGGCG TCCCTGGAGA CCCCGGACCC CCTGGCACTC CAGGCCCTAA AGGGTCCCGG 
GGCAGCCTGG GACCAACGGG TGCTCCTGGA CGCATGGGGG CCCAAGGAGA ACCGGGACTG 
GCTGGTTATG ATGGACACAA AGGCATTGTG GGACCCCTCG GACCTCCTGG ACCAAAAGGC 
GAAAAGGGGG AGCAGGGCGA GGACGGCAAG GCCGAGGGGC CCCCTGGGCC ACCTGGAGAT 
CGGGGCCCTG TGGGTGATCG AGGAGACCGC GGGGAACCGG GGGACCCTGG GTACCCTGGA 
CAGGAGGGTG TGCAAGGCCT CCGTGGAAAG CCAGGCCAGC AGGGCCAACC CGGGCATCCG 
GGACCCCGGG GGCGGCCGGG ACCCAAAGGA TCTAAAGGCG CAGAGGGACC AAAGGGAAAG 
CAAGGCAAGG CGGGGGCCCC AGGCCGGAGG GGGGTCCAGG GCCTGCAGGG GCTGCCAGGG 
CCCCGGGGCG TGGTGGGGAG ACAGGGCCTC GAGGGCATCG CTGGAGCAGA TGGGCTTCCT 
GGCAGGGACG GGCAAGTGGG ACAGCAGGGG GAGCAGGGAG ACGATGGGGA CCCTGGCCCC 
ATGGGCCCTG CTGGGAAGAG AGGAAATCCA GGTGTGGCCG GCTTACCTGG AGCACAGGGA 
CCCCCAGGAT TCAAGGGTGA GAGTGGGTTA CCCGGACAGC TGGGTCCCCC TGGCAAACGA 
GGGACGGAGG GCAGAACGGG GCTCCCTGGA AACCAGGGGG AGCCTGGGTC CAAAGGCCAG 
CCGGGCGACT CTGGTGAGAT GGGCTTCCCA GGAATGGCAG GTCTCTTCGG ACCTAAGGGC 
CCCCCTGGAG ACATCGGCTT CAAAGGCATC CAGGGTCCTC GGGGGCCACC CGGTTTGATG 
GGAAAGGAAG GCATCATCGG GCCCCTCGGA ATCCTAGGAC CTTCGGGACT CCCGGGTCCG 
AAGGGTGACA AAGGCAGCCG TGGGGACTGG GGATTGCAAG GTCCGAGGGG TCCTCCTGGC 
CCCAGAGGGC GGCCCGGCCC CCCGGGTCCT CCAGGGGGTC CTATCCAATT GCAACAAGAT 
GATCTTGGGG TAGCTTTCCA GACGTGGATG GACACCAGTG GAGCACTCAG ACCAGAGGGT 
TACAGCTATC CGGACCGGCT GGTGCTTGAC CAGGGAGGAG AGATCTTTAA AACCTTACAC 
TACCTCAGCA ACCTCATCCA GAGCATTAAG ACGCCCCTGG GCACCAAAGA GAACCCCGCC 
CGGGTCTGCA GGGACCTCAT GGACTGTGAG CAGAAGATGG TGGATGGTAC CTACTGGGTG 
GATCCAAACC TTGGCTGCTC ATCCGACACC ATTGAAGTCT CCTGCAACTT CACGCATGGT 
GGACAGACGT GTCTCAAACC CATCACGGCC TCCAAGGTCG AGTTTGCCAT CAGCCGGGTC 
CAGATGAATT TCCTGCACCT GCTAAGCTCT GAGGTGACCC AGCATATCAC CATCCACTGC 
CTTAACATGA CTGTGTGGCA GGAGGGCACT GGGCAGACCC CAGCCAAGCA GGCCGTGCGC 
TTCCGGGCCT GGAACGGACA GATTTTTGAA GCTGGGGGTC AGTTCCGGCC CGAGGTGTCC 
ATGGATGGCT GCAAGGTCCA AGACGGCCGC TGGCATCAGA CACTCTTCAC CTTCCGGACC 
CAAGACCCCC AACAGCTGCC CATCGTCAGT GTGGACAACC TCCCTCCTGC CTCATCAGGG 
AAGCAGTACC GCCTGGAAGT TGGACCTGCG TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014972495.1
CDS	421..5982
Translation	MGAGSARGARGTATAAAAHGGGFLFSWILVSFACHLASTQGAPEDVDVLQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGSVIPASLGTELALVLSLCSHRVNHAFLFAVRSQKHKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVQLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPLGSQPAGRGPKGTVVPATPTKPQRTSPTNPHQHIAVRGPAQTPPAKLSASNTLDPVLPASLGSSTRTPRPAASQPSQKITATKIPKSLPTKPSAPSTSIVPVKSPHPTQKTAAPSFTKSAPPTQKHVPTVPAKVSRPAEKPIQRNQGMPRPPLPSTQPLPPTTSSSKKPIPTLAQTEAKVTNHASKPASARTSTHKPPPFTALSSSPAPTPGSTRTTRPPATMVPPTSGTSAPRTAPAVPTPGSAPTGNKKPTGSEASKKAGPESSPRKPVPLRPGKAARDVPLSDLTTRPSPRQSQPSQQTTSALVLAPAQFLSSSPRPTSSGYSFFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGEQGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPVGPPGEMGPKGPPGAAGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGRPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGADGLPGRDGQVGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGVAFQTWMDTSGALRPEGYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIVSVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_015117009.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta collagen, type XXVII, alpha 1 (COL27A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015117009.1

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCAGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGTGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGCCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAGACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGACA GAAAGGGGAC CCAGGGCTCT CACCAGGAAA GGCCCACGAT 
GGGGCAAAGG GCGACATGGG CTTGCCTGGG CTGTCCGGGA ATCCAGGACC TCCGGGACGA 
AAGGGACACA AGGGCTATCC TGGACCGGCA GGGCACCCCG GAGAACAGGG GCAGCCAGGA 
CCTGAGGGCA GCCCAGGGGC CAAAGGTTAC CCTGGCAGGC AGGGGTTACC TGGACCGGTA 
GGAGATCCCG GCCCCAAAGG CAGCAGGGGC TACATTGGGC TCCCAGGGCT CTTCGGCCTG 
CCAGGGTCTG ATGGAGAGCG AGGCCTTCCT GGTGTTCCTG GCAAGAGGGG CAAGATGGGT 
ATGCCGGGGT TTCCTGGAGT CTTTGGGGAA AGAGGCCCTC CCGGACTGGA TGGAAACCCT 
GGAGAACTGG GCCTGCCAGG CCCCCCTGGA GTCCCCGGCC TCATTGGTGA CTTGGGAGTG 
TTGGGTCCGA TTGGCTACCC AGGACCCAAG GGCATGAAGG GACTGATGGG CAGCGTGGGG 
GAGCCCGGAC TGAAAGGTGA TAAGGGTGAA CAAGGGGTTC CAGGTGTGTC GGGAGATCCC 
GGATTCCAAG GAGACAAGGG GAGCCAGGGG TTGCCAGGGT TCCCCGGCGC ACGGGGGAAG 
CCAGGGCCTC TGGGCAAAGT TGGAGACAAA GGATCCATTG GGTTTCCCGG GCCCCCTGGA 
CCCGAGGGAT TCCCAGGAGA CATCGGCCCC CCTGGCGACA ACGGCCCAGA AGGCATGAAG 
GGTAAGCCTG GAGCCCGAGG CCTGCCGGGA CCCCGTGGGC AGCTGGGGCC CGAGGGAGAT 
GAGGGACCCA TGGGGCCACC AGGGGCCCCT GGCTTGGAGG GTCAGCCTGG CAGGAAGGGC 
TTTCCTGGGA GGCCCGGCCT GGATGGCGTG AAGGGGGAAC CGGGAGATCC TGGTCGGCCA 
GGGCCTGTGG GAGAGCAGGG ATTTATGGGA TTCATTGGTC TGGTCGGGGA GCCAGGAATC 
GTGGGAGAAA AGGGTGATCG TGGCATGATG GGACCCCCAG GCGTGCCTGG ACCCAAGGGG 
TCGATGGGTC ATCCTGGAAT GCCAGGTGGT ATGGGGACCC CTGGAGAACC TGGACCCCAG 
GGTCCTCCAG GATCTCGAGG CCCACCAGGC ATGAGGGGAG CGAAGGGACG TCGGGGCCCC 
CGAGGACCGG ACGGACCAGC TGGGGAGCAA GGGTCCAGGG GCCTGAAGGG CCCTCCAGGA 
CCCCAGGGCA GACCGGGCCG GCCTGGACAG CAGGGTGTGG CTGGTGAGCA AGGCCACTTG 
GGTTCGCGAG GCTTTCCCGG CATCCCGGGT CCCTCAGGCC CCCCAGGCAC CAAGGGCCTC 
CCAGGAGAAC CGGGCCCTCA GGGACCCCAG GGGCCAGTTG GGCCTCCAGG AGAGATGGGA 
CCTAAGGGGC CGCCGGGTGC AGCGGGAGAA CCGGGCCTTC CTGGGGAAGC TGGGATGAAG 
GGTGACCTTG GACCCCTGGG CACCCCTGGG GAGCAGGGCC TCATTGGGCA ACGGGGAGAG 
CCAGGCCTCG AGGGTGACAG TGGCCCCATG GGACCTGATG GGCTGAAGGG GGACAGGGGA 
GACCCAGGGC CTGATGGAGA ACATGGCGAG AAAGGCCAGG AAGGGCTGAT GGGTGAGGAC 
GGGCCCCCCG GCCCCCCTGG TGTCACTGGT GTCCGGGGTC CTGAAGGAAA ATCAGGGAAG 
CAAGGTGAGA AGGGCCGCAC TGGAGCCAAG GGCGCCAAGG GCTACCAAGG ACAGCTGGGT 
GAGATGGGCG TCCCTGGAGA CCCCGGACCC CCTGGCACTC CAGGCCCTAA AGGGTCCCGG 
GGCAGCCTGG GACCAACGGG TGCTCCTGGA CGCATGGGGG CCCAAGGAGA ACCGGGACTG 
GCTGGTTATG ATGGACACAA AGGCATTGTG GGACCCCTCG GACCTCCTGG ACCAAAAGGC 
GAAAAGGGGG AGCAGGGCGA GGACGGCAAG GCCGAGGGGC CCCCTGGGCC ACCTGGAGAT 
CGGGGCCCTG TGGGTGATCG AGGAGACCGC GGGGAACCGG GGGACCCTGG GTACCCTGGA 
CAGGAGGGTG TGCAAGGCCT CCGTGGAAAG CCAGGCCAGC AGGGCCAACC CGGGCATCCG 
GGACCCCGGG GGCGGCCGGG ACCCAAAGGA TCTAAAGGCG CAGAGGGACC AAAGGGAAAG 
CAAGGCAAGG CGGGGGCCCC AGGCCGGAGG GGGGTCCAGG GCCTGCAGGG GCTGCCAGGG 
CCCCGGGGCG TGGTGGGGAG ACAGGGCCTC GAGGGCATCG CTGGAGCAGA TGGGCTTCCT 
GGCAGGGACG GGCAAGTGGG ACAGCAGGGG GAGCAGGGAG ACGATGGGGA CCCTGGCCCC 
ATGGGCCCTG CTGGGAAGAG AGGAAATCCA GGTGTGGCCG GCTTACCTGG AGCACAGGGA 
CCCCCAGGAT TCAAGGGTGA GAGTGGGTTA CCCGGACAGC TGGGTCCCCC TGGCAAACGA 
GGGACGGAGG GCAGAACGGG GCTCCCTGGA AACCAGGGGG AGCCTGGGTC CAAAGGCCAG 
CCGGGCGACT CTGGTGAGAT GGGCTTCCCA GGAATGGCAG GTCTCTTCGG ACCTAAGGGC 
CCCCCTGGAG ACATCGGCTT CAAAGGCATC CAGGGTCCTC GGGGGCCACC CGGTTTGATG 
GGAAAGGAAG GCATCATCGG GCCCCTCGGA ATCCTAGGAC CTTCGGGACT CCCGGGTCCG 
AAGGGTGACA AAGGCAGCCG TGGGGACTGG GGATTGCAAG GTCCGAGGGG TCCTCCTGGC 
CCCAGAGGGC GGCCCGGCCC CCCGGGTCCT CCAGGGGGTC CTATCCAATT GCAACAAGAT 
GATCTTGGGG TAGCTTTCCA GACGTGGATG GACACCAGTG GAGCACTCAG ACCAGAGGGT 
TACAGCTATC CGGACCGGCT GGTGCTTGAC CAGGGAGGAG AGATCTTTAA AACCTTACAC 
TACCTCAGCA ACCTCATCCA GAGCATTAAG ACGCCCCTGG GCACCAAAGA GAACCCCGCC 
CGGGTCTGCA GGGACCTCAT GGACTGTGAG CAGAAGATGG TGGATGGTAC CTACTGGGTG 
GATCCAAACC TTGGCTGCTC ATCCGACACC ATTGAAGTCT CCTGCAACTT CACGCATGGT 
GGACAGACGT GTCTCAAACC CATCACGGCC TCCAAGGTCG AGTTTGCCAT CAGCCGGGTC 
CAGATGAATT TCCTGCACCT GCTAAGCTCT GAGGTGACCC AGCATATCAC CATCCACTGC 
CTTAACATGA CTGTGTGGCA GGAGGGCACT GGGCAGACCC CAGCCAAGCA GGCCGTGCGC 
TTCCGGGCCT GGAACGGACA GATTTTTGAA GCTGGGGGTC AGTTCCGGCC CGAGGTGTCC 
ATGGATGGCT GCAAGGTCCA AGACGGCCGC TGGCATCAGA CACTCTTCAC CTTCCGGACC 
CAAGACCCCC AACAGCTGCC CATCGTCAGT GTGGACAACC TCCCTCCTGC CTCATCAGGG 
AAGCAGTACC GCCTGGAAGT TGGACCTGCG TGCTTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL27A1 ( XM_015117010.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014972496.1 Macaca mulatta collagen, type XXVII, alpha 1 (COL27A1), transcript variant X2, mRNA.

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CloneID	OMb48771
Clone ID Related Accession (Same CDS sequence)	XM_015117010.1
Accession Version	XM_015117010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805511.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCAGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGTGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGCCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAGACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGCGA CATGGGCTTG CCTGGGCTGT CCGGGAATCC AGGACCTCCG 
GGACGAAAGG GACACAAGGG CTATCCTGGA CCGGCAGGGC ACCCCGGAGA ACAGGGGCAG 
CCAGGACCTG AGGGCAGCCC AGGGGCCAAA GGTTACCCTG GCAGGCAGGG GTTACCTGGA 
CCGGTAGGAG ATCCCGGCCC CAAAGGCAGC AGGGGCTACA TTGGGCTCCC AGGGCTCTTC 
GGCCTGCCAG GGTCTGATGG AGAGCGAGGC CTTCCTGGTG TTCCTGGCAA GAGGGGCAAG 
ATGGGTATGC CGGGGTTTCC TGGAGTCTTT GGGGAAAGAG GCCCTCCCGG ACTGGATGGA 
AACCCTGGAG AACTGGGCCT GCCAGGCCCC CCTGGAGTCC CCGGCCTCAT TGGTGACTTG 
GGAGTGTTGG GTCCGATTGG CTACCCAGGA CCCAAGGGCA TGAAGGGACT GATGGGCAGC 
GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTTCCAGG TGTGTCGGGA 
GATCCCGGAT TCCAAGGAGA CAAGGGGAGC CAGGGGTTGC CAGGGTTCCC CGGCGCACGG 
GGGAAGCCAG GGCCTCTGGG CAAAGTTGGA GACAAAGGAT CCATTGGGTT TCCCGGGCCC 
CCTGGACCCG AGGGATTCCC AGGAGACATC GGCCCCCCTG GCGACAACGG CCCAGAAGGC 
ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCGGGACCCC GTGGGCAGCT GGGGCCCGAG 
GGAGATGAGG GACCCATGGG GCCACCAGGG GCCCCTGGCT TGGAGGGTCA GCCTGGCAGG 
AAGGGCTTTC CTGGGAGGCC CGGCCTGGAT GGCGTGAAGG GGGAACCGGG AGATCCTGGT 
CGGCCAGGGC CTGTGGGAGA GCAGGGATTT ATGGGATTCA TTGGTCTGGT CGGGGAGCCA 
GGAATCGTGG GAGAAAAGGG TGATCGTGGC ATGATGGGAC CCCCAGGCGT GCCTGGACCC 
AAGGGGTCGA TGGGTCATCC TGGAATGCCA GGTGGTATGG GGACCCCTGG AGAACCTGGA 
CCCCAGGGTC CTCCAGGATC TCGAGGCCCA CCAGGCATGA GGGGAGCGAA GGGACGTCGG 
GGCCCCCGAG GACCGGACGG ACCAGCTGGG GAGCAAGGGT CCAGGGGCCT GAAGGGCCCT 
CCAGGACCCC AGGGCAGACC GGGCCGGCCT GGACAGCAGG GTGTGGCTGG TGAGCAAGGC 
CACTTGGGTT CGCGAGGCTT TCCCGGCATC CCGGGTCCCT CAGGCCCCCC AGGCACCAAG 
GGCCTCCCAG GAGAACCGGG CCCTCAGGGA CCCCAGGGGC CAGTTGGGCC TCCAGGAGAG 
ATGGGACCTA AGGGGCCGCC GGGTGCAGCG GGAGAACCGG GCCTTCCTGG GGAAGCTGGG 
ATGAAGGGTG ACCTTGGACC CCTGGGCACC CCTGGGGAGC AGGGCCTCAT TGGGCAACGG 
GGAGAGCCAG GCCTCGAGGG TGACAGTGGC CCCATGGGAC CTGATGGGCT GAAGGGGGAC 
AGGGGAGACC CAGGGCCTGA TGGAGAACAT GGCGAGAAAG GCCAGGAAGG GCTGATGGGT 
GAGGACGGGC CCCCCGGCCC CCCTGGTGTC ACTGGTGTCC GGGGTCCTGA AGGAAAATCA 
GGGAAGCAAG GTGAGAAGGG CCGCACTGGA GCCAAGGGCG CCAAGGGCTA CCAAGGACAG 
CTGGGTGAGA TGGGCGTCCC TGGAGACCCC GGACCCCCTG GCACTCCAGG CCCTAAAGGG 
TCCCGGGGCA GCCTGGGACC AACGGGTGCT CCTGGACGCA TGGGGGCCCA AGGAGAACCG 
GGACTGGCTG GTTATGATGG ACACAAAGGC ATTGTGGGAC CCCTCGGACC TCCTGGACCA 
AAAGGCGAAA AGGGGGAGCA GGGCGAGGAC GGCAAGGCCG AGGGGCCCCC TGGGCCACCT 
GGAGATCGGG GCCCTGTGGG TGATCGAGGA GACCGCGGGG AACCGGGGGA CCCTGGGTAC 
CCTGGACAGG AGGGTGTGCA AGGCCTCCGT GGAAAGCCAG GCCAGCAGGG CCAACCCGGG 
CATCCGGGAC CCCGGGGGCG GCCGGGACCC AAAGGATCTA AAGGCGCAGA GGGACCAAAG 
GGAAAGCAAG GCAAGGCGGG GGCCCCAGGC CGGAGGGGGG TCCAGGGCCT GCAGGGGCTG 
CCAGGGCCCC GGGGCGTGGT GGGGAGACAG GGCCTCGAGG GCATCGCTGG AGCAGATGGG 
CTTCCTGGCA GGGACGGGCA AGTGGGACAG CAGGGGGAGC AGGGAGACGA TGGGGACCCT 
GGCCCCATGG GCCCTGCTGG GAAGAGAGGA AATCCAGGTG TGGCCGGCTT ACCTGGAGCA 
CAGGGACCCC CAGGATTCAA GGGTGAGAGT GGGTTACCCG GACAGCTGGG TCCCCCTGGC 
AAACGAGGGA CGGAGGGCAG AACGGGGCTC CCTGGAAACC AGGGGGAGCC TGGGTCCAAA 
GGCCAGCCGG GCGACTCTGG TGAGATGGGC TTCCCAGGAA TGGCAGGTCT CTTCGGACCT 
AAGGGCCCCC CTGGAGACAT CGGCTTCAAA GGCATCCAGG GTCCTCGGGG GCCACCCGGT 
TTGATGGGAA AGGAAGGCAT CATCGGGCCC CTCGGAATCC TAGGACCTTC GGGACTCCCG 
GGTCCGAAGG GTGACAAAGG CAGCCGTGGG GACTGGGGAT TGCAAGGTCC GAGGGGTCCT 
CCTGGCCCCA GAGGGCGGCC CGGCCCCCCG GGTCCTCCAG GGGGTCCTAT CCAATTGCAA 
CAAGATGATC TTGGGGTAGC TTTCCAGACG TGGATGGACA CCAGTGGAGC ACTCAGACCA 
GAGGGTTACA GCTATCCGGA CCGGCTGGTG CTTGACCAGG GAGGAGAGAT CTTTAAAACC 
TTACACTACC TCAGCAACCT CATCCAGAGC ATTAAGACGC CCCTGGGCAC CAAAGAGAAC 
CCCGCCCGGG TCTGCAGGGA CCTCATGGAC TGTGAGCAGA AGATGGTGGA TGGTACCTAC 
TGGGTGGATC CAAACCTTGG CTGCTCATCC GACACCATTG AAGTCTCCTG CAACTTCACG 
CATGGTGGAC AGACGTGTCT CAAACCCATC ACGGCCTCCA AGGTCGAGTT TGCCATCAGC 
CGGGTCCAGA TGAATTTCCT GCACCTGCTA AGCTCTGAGG TGACCCAGCA TATCACCATC 
CACTGCCTTA ACATGACTGT GTGGCAGGAG GGCACTGGGC AGACCCCAGC CAAGCAGGCC 
GTGCGCTTCC GGGCCTGGAA CGGACAGATT TTTGAAGCTG GGGGTCAGTT CCGGCCCGAG 
GTGTCCATGG ATGGCTGCAA GGTCCAAGAC GGCCGCTGGC ATCAGACACT CTTCACCTTC 
CGGACCCAAG ACCCCCAACA GCTGCCCATC GTCAGTGTGG ACAACCTCCC TCCTGCCTCA 
TCAGGGAAGC AGTACCGCCT GGAAGTTGGA CCTGCGTGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014972496.1
CDS	421..5928
Translation	MGAGSARGARGTATAAAAHGGGFLFSWILVSFACHLASTQGAPEDVDVLQRLGLSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGSVIPASLGTELALVLSLCSHRVNHAFLFAVRSQKHKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVQLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPLGSQPAGRGPKGTVVPATPTKPQRTSPTNPHQHIAVRGPAQTPPAKLSASNTLDPVLPASLGSSTRTPRPAASQPSQKITATKIPKSLPTKPSAPSTSIVPVKSPHPTQKTAAPSFTKSAPPTQKHVPTVPAKVSRPAEKPIQRNQGMPRPPLPSTQPLPPTTSSSKKPIPTLAQTEAKVTNHASKPASARTSTHKPPPFTALSSSPAPTPGSTRTTRPPATMVPPTSGTSAPRTAPAVPTPGSAPTGNKKPTGSEASKKAGPESSPRKPVPLRPGKAARDVPLSDLTTRPSPRQSQPSQQTTSALVLAPAQFLSSSPRPTSSGYSFFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPYGNPGLPGPPGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGEQGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPVGPPGEMGPKGPPGAAGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGRPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGADGLPGRDGQVGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGVAFQTWMDTSGALRPEGYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKVEFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGGQFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIVSVDNLPPASSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_015117010.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta collagen, type XXVII, alpha 1 (COL27A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015117010.1

ORF Insert Sequence:

ATGGGAGCGG GATCGGCGCG GGGGGCCCGA GGCACAGCGA CGGCGGCGGC GGCGCACGGG 
GGGGGGTTTC TCTTCTCCTG GATCTTAGTC TCGTTTGCCT GTCACCTGGC CTCCACCCAA 
GGAGCTCCTG AAGATGTGGA CGTCCTCCAG CGGCTGGGCC TCAGCTGGAC GAAGGCCGGG 
AGCCCTGCAC CCCCGGGAGT CATTCCTTTC CAGTCTGGCT TCATCTTTAC ACAGCGGGCC 
CGGCTCCAGG CTCCCACGGG CTCCGTCATT CCTGCCTCCC TGGGCACAGA GCTGGCGCTG 
GTGCTGAGCC TCTGCTCCCA CCGGGTGAAC CATGCCTTCC TCTTCGCTGT CCGCAGCCAG 
AAACACAAGC TGCAGCTGGG CCTGCAGTTC CTCCCCGGCA AGACGGTCGT CCACCTGGGG 
TCCCGGCGTT CGGTGGCCTT CGACCTCGAC ATGCACGACG GGCGCTGGCA CCACCTGGCC 
CTCGAGCTCC GAGGCCGCAC AGTCACTCTG GTGACCGCCT GCGGGCAGCG CCGGGTGCCT 
GTCCAGCTGC CTTTCCACAG GGACCCTGCA CTCGACCCCG GGGGCTCCTT CCTCTTTGGG 
AAGATGAACC CGCATGCAGT CCAGTTTGAA GGTGCTCTGT GCCAGTTCAG TATCTACCCT 
GTGACGCAGG TCGCTCACAA TTACTGTACC CACCTGAGGA AGCAGTGTGG ACAGGCTGAC 
ACGTACCAGT CCCCACTGGG GCCTCTCTTC TCCCAAGACT CTGGCAGACC TTTCACCTTC 
CAGTCTGACC TCGCCCTGCT AGGCCTGGAG AACTTGACCA CTGCCACACC ACTGGGGTCA 
CAGCCAGCAG GCAGGGGACC CAAGGGGACT GTGGTGCCCG CCACTCCCAC CAAGCCCCAA 
AGGACTAGCC CCACAAACCC TCACCAGCAT ATCGCGGTGC GAGGCCCAGC CCAAACCCCA 
CCTGCCAAGC TGTCAGCCAG TAACACACTT GATCCTGTGC TCCCAGCCTC TCTTGGCAGC 
TCTACCAGAA CGCCTCGCCC TGCAGCCTCT CAACCATCAC AGAAGATCAC AGCCACCAAA 
ATCCCCAAAA GCCTCCCTAC CAAGCCTTCA GCCCCTTCTA CTTCAATTGT CCCCGTCAAA 
AGCCCCCATC CTACCCAGAA AACAGCTGCA CCTTCATTTA CAAAGTCAGC CCCACCCACT 
CAGAAGCATG TACCTACAGT TCCTGCCAAA GTATCCCGTC CCGCAGAGAA GCCCATCCAG 
AGGAACCAAG GAATGCCCAG GCCCCCACTG CCCAGCACCC AGCCCCTACC TCCTACCACC 
AGCTCCTCTA AAAAACCCAT TCCCACACTA GCTCAGACTG AGGCCAAGGT GACTAACCAT 
GCCAGTAAGC CGGCCTCTGC CCGCACCAGC ACCCACAAAC CTCCCCCATT TACTGCTTTA 
TCCTCATCTC CTGCCCCTAC TCCTGGTTCT ACCAGGACGA CTCGGCCACC AGCCACAATG 
GTGCCTCCAA CCTCGGGCAC CAGCGCTCCC AGAACAGCAC CTGCCGTCCC CACTCCTGGC 
TCAGCTCCCA CTGGAAACAA GAAGCCCACT GGATCGGAAG CCTCAAAGAA AGCCGGACCT 
GAGAGCAGCC CCCGGAAGCC CGTCCCCCTC AGACCTGGGA AGGCAGCCAG GGATGTCCCC 
TTGAGCGATC TGACAACCAG GCCTAGCCCC AGACAGTCCC AGCCCAGTCA GCAGACCACT 
TCAGCCCTGG TATTGGCCCC AGCTCAATTC CTGTCCTCCA GCCCCCGGCC CACGAGCAGT 
GGCTATTCGT TCTTCCACCT GGCAGGATCT ACGCCTTTCC CTCTGCTGAT GGGGCCTCCG 
GGGCCCAAGG GAGACTGTGG TTTGCCGGGT CCCCCTGGGC TGCCTGGGCT ACCTGGAATC 
CCTGGTGCAC GTGGGCCTCG GGGTCCTCCT GGGCCTTATG GAAATCCAGG TCTCCCCGGC 
CCTCCTGGAG CCAAAGGCGA CATGGGCTTG CCTGGGCTGT CCGGGAATCC AGGACCTCCG 
GGACGAAAGG GACACAAGGG CTATCCTGGA CCGGCAGGGC ACCCCGGAGA ACAGGGGCAG 
CCAGGACCTG AGGGCAGCCC AGGGGCCAAA GGTTACCCTG GCAGGCAGGG GTTACCTGGA 
CCGGTAGGAG ATCCCGGCCC CAAAGGCAGC AGGGGCTACA TTGGGCTCCC AGGGCTCTTC 
GGCCTGCCAG GGTCTGATGG AGAGCGAGGC CTTCCTGGTG TTCCTGGCAA GAGGGGCAAG 
ATGGGTATGC CGGGGTTTCC TGGAGTCTTT GGGGAAAGAG GCCCTCCCGG ACTGGATGGA 
AACCCTGGAG AACTGGGCCT GCCAGGCCCC CCTGGAGTCC CCGGCCTCAT TGGTGACTTG 
GGAGTGTTGG GTCCGATTGG CTACCCAGGA CCCAAGGGCA TGAAGGGACT GATGGGCAGC 
GTGGGGGAGC CCGGACTGAA AGGTGATAAG GGTGAACAAG GGGTTCCAGG TGTGTCGGGA 
GATCCCGGAT TCCAAGGAGA CAAGGGGAGC CAGGGGTTGC CAGGGTTCCC CGGCGCACGG 
GGGAAGCCAG GGCCTCTGGG CAAAGTTGGA GACAAAGGAT CCATTGGGTT TCCCGGGCCC 
CCTGGACCCG AGGGATTCCC AGGAGACATC GGCCCCCCTG GCGACAACGG CCCAGAAGGC 
ATGAAGGGTA AGCCTGGAGC CCGAGGCCTG CCGGGACCCC GTGGGCAGCT GGGGCCCGAG 
GGAGATGAGG GACCCATGGG GCCACCAGGG GCCCCTGGCT TGGAGGGTCA GCCTGGCAGG 
AAGGGCTTTC CTGGGAGGCC CGGCCTGGAT GGCGTGAAGG GGGAACCGGG AGATCCTGGT 
CGGCCAGGGC CTGTGGGAGA GCAGGGATTT ATGGGATTCA TTGGTCTGGT CGGGGAGCCA 
GGAATCGTGG GAGAAAAGGG TGATCGTGGC ATGATGGGAC CCCCAGGCGT GCCTGGACCC 
AAGGGGTCGA TGGGTCATCC TGGAATGCCA GGTGGTATGG GGACCCCTGG AGAACCTGGA 
CCCCAGGGTC CTCCAGGATC TCGAGGCCCA CCAGGCATGA GGGGAGCGAA GGGACGTCGG 
GGCCCCCGAG GACCGGACGG ACCAGCTGGG GAGCAAGGGT CCAGGGGCCT GAAGGGCCCT 
CCAGGACCCC AGGGCAGACC GGGCCGGCCT GGACAGCAGG GTGTGGCTGG TGAGCAAGGC 
CACTTGGGTT CGCGAGGCTT TCCCGGCATC CCGGGTCCCT CAGGCCCCCC AGGCACCAAG 
GGCCTCCCAG GAGAACCGGG CCCTCAGGGA CCCCAGGGGC CAGTTGGGCC TCCAGGAGAG 
ATGGGACCTA AGGGGCCGCC GGGTGCAGCG GGAGAACCGG GCCTTCCTGG GGAAGCTGGG 
ATGAAGGGTG ACCTTGGACC CCTGGGCACC CCTGGGGAGC AGGGCCTCAT TGGGCAACGG 
GGAGAGCCAG GCCTCGAGGG TGACAGTGGC CCCATGGGAC CTGATGGGCT GAAGGGGGAC 
AGGGGAGACC CAGGGCCTGA TGGAGAACAT GGCGAGAAAG GCCAGGAAGG GCTGATGGGT 
GAGGACGGGC CCCCCGGCCC CCCTGGTGTC ACTGGTGTCC GGGGTCCTGA AGGAAAATCA 
GGGAAGCAAG GTGAGAAGGG CCGCACTGGA GCCAAGGGCG CCAAGGGCTA CCAAGGACAG 
CTGGGTGAGA TGGGCGTCCC TGGAGACCCC GGACCCCCTG GCACTCCAGG CCCTAAAGGG 
TCCCGGGGCA GCCTGGGACC AACGGGTGCT CCTGGACGCA TGGGGGCCCA AGGAGAACCG 
GGACTGGCTG GTTATGATGG ACACAAAGGC ATTGTGGGAC CCCTCGGACC TCCTGGACCA 
AAAGGCGAAA AGGGGGAGCA GGGCGAGGAC GGCAAGGCCG AGGGGCCCCC TGGGCCACCT 
GGAGATCGGG GCCCTGTGGG TGATCGAGGA GACCGCGGGG AACCGGGGGA CCCTGGGTAC 
CCTGGACAGG AGGGTGTGCA AGGCCTCCGT GGAAAGCCAG GCCAGCAGGG CCAACCCGGG 
CATCCGGGAC CCCGGGGGCG GCCGGGACCC AAAGGATCTA AAGGCGCAGA GGGACCAAAG 
GGAAAGCAAG GCAAGGCGGG GGCCCCAGGC CGGAGGGGGG TCCAGGGCCT GCAGGGGCTG 
CCAGGGCCCC GGGGCGTGGT GGGGAGACAG GGCCTCGAGG GCATCGCTGG AGCAGATGGG 
CTTCCTGGCA GGGACGGGCA AGTGGGACAG CAGGGGGAGC AGGGAGACGA TGGGGACCCT 
GGCCCCATGG GCCCTGCTGG GAAGAGAGGA AATCCAGGTG TGGCCGGCTT ACCTGGAGCA 
CAGGGACCCC CAGGATTCAA GGGTGAGAGT GGGTTACCCG GACAGCTGGG TCCCCCTGGC 
AAACGAGGGA CGGAGGGCAG AACGGGGCTC CCTGGAAACC AGGGGGAGCC TGGGTCCAAA 
GGCCAGCCGG GCGACTCTGG TGAGATGGGC TTCCCAGGAA TGGCAGGTCT CTTCGGACCT 
AAGGGCCCCC CTGGAGACAT CGGCTTCAAA GGCATCCAGG GTCCTCGGGG GCCACCCGGT 
TTGATGGGAA AGGAAGGCAT CATCGGGCCC CTCGGAATCC TAGGACCTTC GGGACTCCCG 
GGTCCGAAGG GTGACAAAGG CAGCCGTGGG GACTGGGGAT TGCAAGGTCC GAGGGGTCCT 
CCTGGCCCCA GAGGGCGGCC CGGCCCCCCG GGTCCTCCAG GGGGTCCTAT CCAATTGCAA 
CAAGATGATC TTGGGGTAGC TTTCCAGACG TGGATGGACA CCAGTGGAGC ACTCAGACCA 
GAGGGTTACA GCTATCCGGA CCGGCTGGTG CTTGACCAGG GAGGAGAGAT CTTTAAAACC 
TTACACTACC TCAGCAACCT CATCCAGAGC ATTAAGACGC CCCTGGGCAC CAAAGAGAAC 
CCCGCCCGGG TCTGCAGGGA CCTCATGGAC TGTGAGCAGA AGATGGTGGA TGGTACCTAC 
TGGGTGGATC CAAACCTTGG CTGCTCATCC GACACCATTG AAGTCTCCTG CAACTTCACG 
CATGGTGGAC AGACGTGTCT CAAACCCATC ACGGCCTCCA AGGTCGAGTT TGCCATCAGC 
CGGGTCCAGA TGAATTTCCT GCACCTGCTA AGCTCTGAGG TGACCCAGCA TATCACCATC 
CACTGCCTTA ACATGACTGT GTGGCAGGAG GGCACTGGGC AGACCCCAGC CAAGCAGGCC 
GTGCGCTTCC GGGCCTGGAA CGGACAGATT TTTGAAGCTG GGGGTCAGTT CCGGCCCGAG 
GTGTCCATGG ATGGCTGCAA GGTCCAAGAC GGCCGCTGGC ATCAGACACT CTTCACCTTC 
CGGACCCAAG ACCCCCAACA GCTGCCCATC GTCAGTGTGG ACAACCTCCC TCCTGCCTCA 
TCAGGGAAGC AGTACCGCCT GGAAGTTGGA CCTGCGTGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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COL27A1 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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