MID2 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	MID2
Entrez Gene ID	703281
Full Name	midline 2
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015127966.1	XP_014983452.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_015127966.2	XP_014983452.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_015127965.1	XP_014983451.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_015127967.1	XP_014983453.1	probable E3 ubiquitin-protein ligase MID2 isoform X3
XM_015127965.2	XP_014983451.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_015127967.2	XP_014983453.1	probable E3 ubiquitin-protein ligase MID2 isoform X3

Pathways from BioSystems
Gene Ontology

The following MID2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMb56533	XM_015127965.2 Latest version!	Macaca mulatta midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMb56534	XM_015127966.2 Latest version!	Macaca mulatta midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMb56535	XM_015127967.2 Latest version!	Macaca mulatta midline 2 (MID2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OMb56534	XM_015127966.1	Macaca mulatta midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMb56533	XM_015127965.1	Macaca mulatta midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMb56535	XM_015127967.1	Macaca mulatta midline 2 (MID2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID2 ( XM_015127965.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014983451.1 Macaca mulatta midline 2 (MID2), transcript variant X1, mRNA.

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CloneID	OMb56533
Clone ID Related Accession (Same CDS sequence)	XM_015127965.1 , XM_015127965.2
Accession Version	XM_015127965.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014806007.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTG CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGT 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AGCCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA TACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
TTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014983451.1
CDS	574..2781
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_015127965.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015127965.1

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTG CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGT 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AGCCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA TACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
TTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_015127965.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014983451.1 Macaca mulatta midline 2 (MID2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMb56533
Clone ID Related Accession (Same CDS sequence)	XM_015127965.1 , XM_015127965.2
Accession Version	XM_015127965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015127965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTG CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGT 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AGCCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA TACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
TTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014983451.1
CDS	579..2786
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_015127965.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015127965.2

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTG CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGT 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AGCCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA TACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
TTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_015127966.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014983452.1 Macaca mulatta midline 2 (MID2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMb56534
Clone ID Related Accession (Same CDS sequence)	XM_015127966.1 , XM_015127966.2
Accession Version	XM_015127966.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014806007.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTG 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGT AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AGCCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC TTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014983452.1
CDS	578..2695
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_015127966.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015127966.1

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTG 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGT AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AGCCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC TTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_015127966.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014983452.1 Macaca mulatta midline 2 (MID2), transcript variant X2, mRNA.

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CloneID	OMb56534
Clone ID Related Accession (Same CDS sequence)	XM_015127966.1 , XM_015127966.2
Accession Version	XM_015127966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015127966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTG 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGT AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AGCCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC TTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014983452.1
CDS	578..2695
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_015127966.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015127966.2

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTG 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGT AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AGCCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA TACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC TTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_015127967.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014983453.1 Macaca mulatta midline 2 (MID2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMb56535
Clone ID Related Accession (Same CDS sequence)	XM_015127967.1 , XM_015127967.2
Accession Version	XM_015127967.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014806007.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTG CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGT 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AGCCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA TACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
TTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014983453.1
CDS	157..2304
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_015127967.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 2 (MID2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015127967.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTG CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGT 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AGCCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA TACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
TTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_015127967.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014983453.1 Macaca mulatta midline 2 (MID2), transcript variant X3, mRNA.

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CloneID	OMb56535
Clone ID Related Accession (Same CDS sequence)	XM_015127967.1 , XM_015127967.2
Accession Version	XM_015127967.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041774.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015127967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTG CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGT 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AGCCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA TACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
TTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014983453.1
CDS	184..2331
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_015127967.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta midline 2 (MID2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015127967.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCA 
CTTCTGCTCC CTTGTGCTCA TAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCAGCACC GCCATGTCTA GTGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCGAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCACGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTTAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ACACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ACATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTG CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGT 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AGCCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA TACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
TTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MID2 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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