IARS cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS
Entrez Gene ID	677705
Full Name	isoleucyl-tRNA synthetase
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015117256.2	XP_014972742.1	isoleucine--tRNA ligase, cytoplasmic
XM_015117256.1	XP_014972742.1	isoleucine--tRNA ligase, cytoplasmic
XM_015117255.2	XP_014972741.1	isoleucine--tRNA ligase, cytoplasmic
XM_015117255.1	XP_014972741.1	isoleucine--tRNA ligase, cytoplasmic

Pathways from BioSystems

Homologies

	IARS	XP_001107299.2
Kluyveromyces lactis	KLLA0E20549g	XP_454879.1
Eremothecium gossypii	AGOS_AER394W	NP_985249.2
Magnaporthe oryzae (rice blast fungus)	MGG_03220	XP_003716759.1
Mus musculus (house mouse)	Iars	NP_742012.2
Gallus gallus (chicken)	IARS	XP_414300.3
Danio rerio (zebrafish)	iars	NP_956190.1
Saccharomyces cerevisiae (baker's yeast)	ILS1	NP_009477.1
Schizosaccharomyces pombe (fission yeast)	irs1	NP_595569.1
Rattus norvegicus (Norway rat)	Iars	NP_001094042.1
Homo sapiens (human)	IARS	NP_038203.2
Drosophila melanogaster (fruit fly)	Aats-ile	NP_788565.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002101	XP_320939.3
Neurospora crassa	NCU03575	XP_956014.1
Arabidopsis thaliana (thale cress)	AT4G10320	NP_192770.2
Xenopus tropicalis (tropical clawed frog)	iars	NP_001090690.1
Pan troglodytes (chimpanzee)	IARS	XP_003312212.1
Canis lupus familiaris (dog)	IARS	XP_541332.3
Bos taurus (cattle)	IARS	NP_001094539.1
Caenorhabditis elegans (roundworm)	iars-1	NP_501914.1

Gene Ontology

The following IARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMb48945	XM_015117255.1 Latest version!	Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMb48945	XM_015117256.1 Latest version!	Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OMb71884	XM_015117256.2 Latest version!	Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMb71884	XM_015117255.2 Latest version!	Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS ( XM_015117256.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014972742.1 Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMb48945
Clone ID Related Accession (Same CDS sequence)	XM_015117256.1 , XM_015117255.1
Accession Version	XM_015117256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805514.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCGG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCG ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAGT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAGGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTGTCC AACTTCGAGT CACACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACGTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTCTG TGTCAATCCA 
GAAATGCAAT ATGTGAAGAT TAAAGATGTT GCCAGAGGAA AATTATTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTTTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTTCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCAT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTGGTTGAC AACTACGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTTGGTGCT GATGACTATC GGATCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCCGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAGCG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC AGCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT GATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAAGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACACCTAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTAGTATGC 
GTTGGGTCAG TGGCAGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGCGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGAA AGGGATCCTT GCACCGCGTC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGGAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGACTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGTCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTCGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGTAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTGTATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAGGAGG GTGTGCGGGA TGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTT CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CTGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAAACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT GGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTGAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTCCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATCCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAGGAA AACAGAGAGT 
GCAATGTCTC GGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGGGA CCGAAAAACC 
ATTCCCATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGACTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AATGAGGAAC TGGAGCAATT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAACTG 
CATGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAG 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAGTCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCGAAGTC GGAAGGAAGA 
TATCTGAATA ATGTTATTGA AAGCCACACA GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCCT ATCCAGTTTC TCCATCAGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT GTTGCTCCTG 
GAAAATCCAA AAGGTGATAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
GAAACCGACC TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCGGG ATCGGCTCCC 
TCTCTGATCA GCAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAGAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAAGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTATTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014972742.1
CDS	145..3933
Translation	MVQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWRSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGKLFILMEARLSALYKLESDYEILERFPGSYLKGKKYRPLFDYFMKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGADDYRICMDFNIIRKDSLPVCPVDASGCFTAEVTDFAGQYVKDADKSIIRTLKEQGRLLVASTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLKNNDLCYWVPELVREKRFGNWLKDARDWAVSRNRYWGTPIPLWVSDDFEEVVCVGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRVSEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDRKTESAMSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSNEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCSLVPTDEITVYYKAKSEGRYLNNVIESHTEFIFATIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNETDLLSLSGKTLCVTAGSAPSLISSSSTLLCQYINLQLLNAEPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	XM_015117256.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015117256.1

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCGG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCG ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAGT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAGGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTGTCC AACTTCGAGT CACACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACGTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTCTG TGTCAATCCA 
GAAATGCAAT ATGTGAAGAT TAAAGATGTT GCCAGAGGAA AATTATTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTTTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTTCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCAT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTGGTTGAC AACTACGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTTGGTGCT GATGACTATC GGATCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCCGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAGCG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC AGCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT GATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAAGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACACCTAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTAGTATGC 
GTTGGGTCAG TGGCAGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGCGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGAA AGGGATCCTT GCACCGCGTC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGGAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGACTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGTCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTCGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGTAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTGTATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAGGAGG GTGTGCGGGA TGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTT CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CTGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAAACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT GGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTGAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTCCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATCCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAGGAA AACAGAGAGT 
GCAATGTCTC GGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGGGA CCGAAAAACC 
ATTCCCATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGACTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AATGAGGAAC TGGAGCAATT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAACTG 
CATGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAG 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAGTCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCGAAGTC GGAAGGAAGA 
TATCTGAATA ATGTTATTGA AAGCCACACA GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCCT ATCCAGTTTC TCCATCAGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT GTTGCTCCTG 
GAAAATCCAA AAGGTGATAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
GAAACCGACC TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCGGG ATCGGCTCCC 
TCTCTGATCA GCAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAGAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAAGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTATTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_015117255.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014972741.1 Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMb48945
Clone ID Related Accession (Same CDS sequence)	XM_015117256.1 , XM_015117255.1
Accession Version	XM_015117255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805514.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCGG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCG ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAGT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAGGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTGTCC AACTTCGAGT CACACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACGTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTCTG TGTCAATCCA 
GAAATGCAAT ATGTGAAGAT TAAAGATGTT GCCAGAGGAA AATTATTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTTTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTTCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCAT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTGGTTGAC AACTACGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTTGGTGCT GATGACTATC GGATCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCCGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAGCG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC AGCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT GATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAAGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACACCTAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTAGTATGC 
GTTGGGTCAG TGGCAGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGCGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGAA AGGGATCCTT GCACCGCGTC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGGAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGACTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGTCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTCGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGTAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTGTATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAGGAGG GTGTGCGGGA TGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTT CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CTGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAAACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT GGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTGAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTCCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATCCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAGGAA AACAGAGAGT 
GCAATGTCTC GGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGGGA CCGAAAAACC 
ATTCCCATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGACTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AATGAGGAAC TGGAGCAATT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAACTG 
CATGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAG 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAGTCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCGAAGTC GGAAGGAAGA 
TATCTGAATA ATGTTATTGA AAGCCACACA GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCCT ATCCAGTTTC TCCATCAGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT GTTGCTCCTG 
GAAAATCCAA AAGGTGATAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
GAAACCGACC TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCGGG ATCGGCTCCC 
TCTCTGATCA GCAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAGAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAAGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTATTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014972741.1
CDS	149..3937
Translation	MVQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWRSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGKLFILMEARLSALYKLESDYEILERFPGSYLKGKKYRPLFDYFMKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGADDYRICMDFNIIRKDSLPVCPVDASGCFTAEVTDFAGQYVKDADKSIIRTLKEQGRLLVASTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLKNNDLCYWVPELVREKRFGNWLKDARDWAVSRNRYWGTPIPLWVSDDFEEVVCVGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRVSEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDRKTESAMSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSNEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCSLVPTDEITVYYKAKSEGRYLNNVIESHTEFIFATIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNETDLLSLSGKTLCVTAGSAPSLISSSSTLLCQYINLQLLNAEPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	XM_015117255.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015117255.1

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCGG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCG ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAGT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAGGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTGTCC AACTTCGAGT CACACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACGTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTCTG TGTCAATCCA 
GAAATGCAAT ATGTGAAGAT TAAAGATGTT GCCAGAGGAA AATTATTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTTTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTTCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCAT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTGGTTGAC AACTACGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTTGGTGCT GATGACTATC GGATCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCCGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAGCG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC AGCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT GATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAAGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACACCTAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTAGTATGC 
GTTGGGTCAG TGGCAGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGCGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGAA AGGGATCCTT GCACCGCGTC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGGAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGACTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGTCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTCGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGTAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTGTATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAGGAGG GTGTGCGGGA TGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTT CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CTGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAAACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT GGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTGAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTCCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATCCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAGGAA AACAGAGAGT 
GCAATGTCTC GGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGGGA CCGAAAAACC 
ATTCCCATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGACTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AATGAGGAAC TGGAGCAATT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAACTG 
CATGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAG 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAGTCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCGAAGTC GGAAGGAAGA 
TATCTGAATA ATGTTATTGA AAGCCACACA GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCCT ATCCAGTTTC TCCATCAGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT GTTGCTCCTG 
GAAAATCCAA AAGGTGATAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
GAAACCGACC TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCGGG ATCGGCTCCC 
TCTCTGATCA GCAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAGAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAAGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTATTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_015117256.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014972742.1 Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

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CloneID	OMb71884
Clone ID Related Accession (Same CDS sequence)	XM_015117256.2 , XM_015117255.2
Accession Version	XM_015117256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015117256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCGG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCG ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAGT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAGGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTGTCC AACTTCGAGT CACACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACGTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTCTG TGTCAATCCA 
GAAATGCAAT ATGTGAAGAT TAAAGATGTT GCCAGAGGAA AATTATTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTTTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTTCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCAT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTGGTTGAC AACTACGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTTGGTGCT GATGACTATC GGATCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCCGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAGCG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC AGCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT GATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAAGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACACCTAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTAGTATGC 
GTTGGGTCAG TGGCAGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGCGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGAA AGGGATCCTT GCACCGCGTC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGGAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGACTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGTCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTCGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGTAAAA GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTGTATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAGGAGG GTGTGCGGGA TGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTT CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CTGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAAACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT GGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTGAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTCCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATCCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAGGAA AACAGAGAGT 
GCAATGTCTC GGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGGGA CCGAAAAACC 
ATTCCCATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGACTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AATGAGGAAC TGGAGCAATT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAACTG 
CATGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAG 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAGTCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCGAAGTC GGAAGGAAGA 
TATCTGAATA ATGTTATTGA AAGCCACACA GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCCT ATCCAGTTTC TCCATCAGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT GTTGCTCCTG 
GAAAATCCAA AAGGTGATAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
GAAACCGACC TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA GCAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAGAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCGCTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAAGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTATTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014972742.1
CDS	121..3909
Translation	MVQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWRSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGKLFILMEARLSALYKLESDYEILERFPGSYLKGKKYRPLFDYFMKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGADDYRICMDFNIIRKDSLPVCPVDASGCFTAEVTDFAGQYVKDADKSIIRTLKEQGRLLVASTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLKNNDLCYWVPELVREKRFGNWLKDARDWAVSRNRYWGTPIPLWVSDDFEEVVCVGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRVSEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDRKTESAMSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSNEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCSLVPTDEITVYYKAKSEGRYLNNVIESHTEFIFATIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNETDLLSLSGKTLCVTAGSAPSLISSSSTLLCQYINLQLLNAEPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	XM_015117256.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015117256.2

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCGG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCG ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAGT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAGGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTGTCC AACTTCGAGT CACACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACGTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTCTG TGTCAATCCA 
GAAATGCAAT ATGTGAAGAT TAAAGATGTT GCCAGAGGAA AATTATTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTTTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTTCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCAT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTGGTTGAC AACTACGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTTGGTGCT GATGACTATC GGATCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCCGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAGCG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC AGCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT GATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAAGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACACCTAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTAGTATGC 
GTTGGGTCAG TGGCAGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGCGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGAA AGGGATCCTT GCACCGCGTC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGGAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGACTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGTCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTCGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGTAAAA GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTGTATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAGGAGG GTGTGCGGGA TGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTT CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CTGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAAACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT GGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTGAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTCCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATCCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAGGAA AACAGAGAGT 
GCAATGTCTC GGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGGGA CCGAAAAACC 
ATTCCCATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGACTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AATGAGGAAC TGGAGCAATT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAACTG 
CATGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAG 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAGTCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCGAAGTC GGAAGGAAGA 
TATCTGAATA ATGTTATTGA AAGCCACACA GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCCT ATCCAGTTTC TCCATCAGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT GTTGCTCCTG 
GAAAATCCAA AAGGTGATAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
GAAACCGACC TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA GCAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAGAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCGCTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAAGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTATTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_015117255.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014972741.1 Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

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CloneID	OMb71884
Clone ID Related Accession (Same CDS sequence)	XM_015117256.2 , XM_015117255.2
Accession Version	XM_015117255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041768.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015117255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCGG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCG ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAGT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAGGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTGTCC AACTTCGAGT CACACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACGTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTCTG TGTCAATCCA 
GAAATGCAAT ATGTGAAGAT TAAAGATGTT GCCAGAGGAA AATTATTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTTTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTTCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCAT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTGGTTGAC AACTACGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTTGGTGCT GATGACTATC GGATCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCCGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAGCG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC AGCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT GATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAAGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACACCTAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTAGTATGC 
GTTGGGTCAG TGGCAGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGCGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGAA AGGGATCCTT GCACCGCGTC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGGAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGACTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGTCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTCGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGTAAAA GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTGTATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAGGAGG GTGTGCGGGA TGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTT CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CTGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAAACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT GGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTGAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTCCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATCCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAGGAA AACAGAGAGT 
GCAATGTCTC GGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGGGA CCGAAAAACC 
ATTCCCATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGACTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AATGAGGAAC TGGAGCAATT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAACTG 
CATGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAG 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAGTCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCGAAGTC GGAAGGAAGA 
TATCTGAATA ATGTTATTGA AAGCCACACA GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCCT ATCCAGTTTC TCCATCAGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT GTTGCTCCTG 
GAAAATCCAA AAGGTGATAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
GAAACCGACC TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA GCAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAGAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCGCTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAAGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTATTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014972741.1
CDS	149..3937
Translation	MVQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWRSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGKLFILMEARLSALYKLESDYEILERFPGSYLKGKKYRPLFDYFMKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGADDYRICMDFNIIRKDSLPVCPVDASGCFTAEVTDFAGQYVKDADKSIIRTLKEQGRLLVASTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLKNNDLCYWVPELVREKRFGNWLKDARDWAVSRNRYWGTPIPLWVSDDFEEVVCVGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRVSEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDRKTESAMSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSNEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCSLVPTDEITVYYKAKSEGRYLNNVIESHTEFIFATIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNETDLLSLSGKTLCVTAGSAPSLISSSSTLLCQYINLQLLNAEPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	XM_015117255.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015117255.2

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCGG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCG ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG TCATGGCTTA CCTGTGGAGT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAGGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTGTCC AACTTCGAGT CACACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACGTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTCTG TGTCAATCCA 
GAAATGCAAT ATGTGAAGAT TAAAGATGTT GCCAGAGGAA AATTATTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTTTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTTCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCAT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTGGTTGAC AACTACGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTTGGTGCT GATGACTATC GGATCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCCGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAGCG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC AGCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT GATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAAGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAGT TTCCAGAAAC 
AGATACTGGG GCACACCTAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTAGTATGC 
GTTGGGTCAG TGGCAGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGCGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGAA AGGGATCCTT GCACCGCGTC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGGAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGACTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGTCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTCGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGTAAAA GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTGTATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAGGAGG GTGTGCGGGA TGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTT CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CTGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAAACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT GGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTGAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTCCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATCCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAGGAA AACAGAGAGT 
GCAATGTCTC GGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGGGA CCGAAAAACC 
ATTCCCATAA AGTATCCTTT GAAAGAAATC GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGACTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AATGAGGAAC TGGAGCAATT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAACTG 
CATGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAG 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAGTCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCGAAGTC GGAAGGAAGA 
TATCTGAATA ATGTTATTGA AAGCCACACA GAGTTCATAT TTGCCACCAT AAAGGCTCCC 
TTGAAACCCT ATCCAGTTTC TCCATCAGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT GTTGCTCCTG 
GAAAATCCAA AAGGTGATAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
GAAACCGACC TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA GCAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAGAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCGCTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAAGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTATTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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IARS cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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