DENND5B cDNA ORF clone, Latimeria chalumnae(coelacanth)

Gene
Clones
gRNAs

Summary

Gene Symbol	DENND5B
Entrez Gene ID	102357803
Full Name	DENN domain containing 5B
Gene Type	protein-coding
Organism	Latimeria chalumnae(coelacanth)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014492073.1	XP_014347559.1	DENN domain-containing protein 5B

Pathways from BioSystems
Gene Ontology

The following DENND5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa33124	XM_014492073.1 Latest version!	Latimeria chalumnae DENN/MADD domain containing 5B (DENND5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DENND5B ( XM_014492073.1 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_014347559.1 Latimeria chalumnae DENN/MADD domain containing 5B (DENND5B), mRNA.

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CloneID	OLa33124
Clone ID Related Accession (Same CDS sequence)	XM_014492073.1
Accession Version	XM_014492073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	DENN domain-containing protein 5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819775.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGAGCGTGA GCGCCCCCGG CTCGGGATCG GCTCCCTGCC GCTTCGCTCA TTACTTCGTG 
ATCTGCGGCA TCGACACGGA CAGCGGGCTG GAGCCCGACG AGCTGGCCGG AGAAAACTTT 
GAGCAGAGCC CGCTGAAAAG AACTTTCAAG TCCAAAGTTT TGGCCCACTA CCCTCAGAAC 
GTGGAGTGGA ATCCTTTTGA CCAAGATGCT GTTAACATGC TCTGCATGCC CAAAGGCCTT 
TCCTTCCGAA CGCAGATGGA GAACAGAGAT CCTCAGTTCC ACTCCTACAT CATCACCCGT 
GAGGATGGCT CCCGCACCTA CGGCTTCGTC CTCACCTTCT ACGAGGAGGT GACTTGCAAG 
CAGATCTGCA CAGCCATGCA GACCCTGTAC CAGATGCACA ATGCTGAGCA CTACAGTAGC 
GTCTGCGCCT CCTCCTCGTG CAGTATGGAC TCGCTGGCCA GCAGCCTCGA CGAGGGGGAC 
AACACCTCCC TCTCCAAACT CCAGCGCTGC AACTCCTACG ACATCAGCAG GGACACGCTC 
TACGTCTCCA AGTGCATCTG CCTCATCACC CCTCTCCCTT TCATGCGGGC CTGCCGGAAG 
TTCCTCAGTC AACTTCACAG GGCCGTCACC TCCCAGCAGC CCCCTCCGTT GCCCCTGGAG 
AGCTACATCC ATAACATCCT GTACGAGGTG CCGCTGCCGC CTCCCGGCAG GTCGTTGAAG 
TTCTACGGGG TTTATGAACC CATTGTCTGC CAGCGACCAG GCCCCAGCGA GTTGCCTCTG 
TCCGACTACC CTCTCCGTGA AGTCTTTGAG CTGCTTGGGG TCGAAAACCT GGTGCAAGTC 
TTCAGCTGTG TACTGCTAGA GATGCAGATT CTTCTCTACT CGCAAGATTA CCAGCGGCTG 
ATGACAGTAG CAGAAGGGAT TACAACACTC TTGTTCCCCT TTCAGTGGCA GCATGTCTAC 
GTTCCTATCC TTCCTGCCTC CCTTCTCCAT TTCCTGGATG CACCTGTCCC CTATCTAATG 
GGTCTCCAGT CCAAAGAAGG AACTGACCGC TCCAAGCTAG AACTTCCACA AGAGGCTAAC 
TTATGTTTCG TAGACATCGA TAACCACTTC ATTGAGCTGC CGGAGGATTT TCCACAGTTC 
CCCAACAAGC TGGAATTCAT TCAGGAAATC TCGGAGGTCC TCCTTCAGTT TGGAATCCCC 
CCCGAGGGTA GCCTGCACTG CAGTGAGAGT GCCACCAAGC TGAAGAGCTT GGTCCTGGAT 
GACCTGGCAG ATGACAAGAA GAATGGCAAC ATCCCCGCCA ACGTGTATGA GCTTCTCAAA 
GGCAACGAAA CCATCGCCCG GCTCCAAGCC CTGGCTAAGC GAACGGGAGT GAGGGTGGAC 
AAGATTGAGA TCACCAGGAT GCTGGCTGAG AAGGAGGAGA AGGACTCCAA GCTGCCTTGT 
GACTGGGAAG AACTGCGGGA TTACAAGCTG AACGTGCAGG TCCGTGAGGT CTTTGCCAGC 
CGCTTCACGC AGATGTTTGC GGACTATGAG GCCTTTGTGA TCCTGACGGC GCAGGACTTG 
GACTCCTGGC TCACAAATCG AGAGCAGATG CAGAACTTTG ACAAAGCATC CTTTCTGTCC 
GACCAGCCTG AACCTTACCT CCCCTTCCTC TCGCACTTCA TCGAAACCCA GATGTTTGCC 
ACATTCATCG ACAATAAGAT CATGTCGCAG TGGGAGGAGA AGGACCCCTT ACTCAGAGTG 
CTTGACTCGC GCATTGAGAA GATAAGGCTT TACAGCGTCC GGGCACCTGC CCTTCGAACA 
TCCATGTATC AAAAATGCAC TGTGTTAAAA GAAGCAGCTC AGTCTATTGA GCAGCGGTTA 
GCAAAGATCG ATCACACTGC CATTCACCCA CACTTGCTTG ACATGAAGAT CGGACAAGGC 
AAGTACGAGC AAGGCTTCTT CCCCAAGCTG CAGTCTGACG TGCTGGCCAC GGGACCGACC 
AATAACAACC GTTGGGCCAG CCGAAGCGCC ACAGCACAGC GCCGTAAAGA CCGTCTCCGG 
CAGCACTCGG AGCACCTCGG GCTGGACAAT GATTTAAGAG AGGTACGGTA TCTAATGCAT 
GGAGGGTTGA CCAGAAGGGG ATTGGAGGGC TCAGGTATTA GCACTGGCAC GCGGACCCTT 
TCACCTCTAG TATCAAATGG TTCTCTTAAC ATCCTGTCAT CAGAGGGGCT TCTGGTCCAG 
CAGAATTCCA TGGCCTTCTG GGAGTGGGAT CAGGGGTCAC CACCCCCTCC TGCACGACCT 
CCTAAAAAGT CATTCTCTGA GTGCTGCCTG ACCAAAAGGA TGTTAGTGGA GAAGATGGGA 
CACGAGGCGG TGGAACTGGG ACACGGGGAG GCCAACATCA CCGGCCTGGA AGAGAACACC 
CTGATCGCCA GTCTGTGTGA CCTACTGGAG AGGATCTGGA GCCATGGCCT ACAGATCAAG 
CAGGGGAAGT CTGCTTTGTG GTCTCACCTC CATCAGTACC AGGAGAGAGA GGAGAAGCAA 
GATCCAGTAC TGGAATCTCC AGTGTCCCTT GGACCAGAGA GAAGAAAATC TGAATCGGGC 
ATTACGTTGC CTTCACTCAG GGTATCCCTT ATCCACGACA TGAGGCACAT CCAGAATATG 
AGTGAGATAA AGACAGATGT TGGTCGGGCT CGAGCTTGGA TCAGGCTGTC CCTGGAAAAG 
AAGCTCTTAT CTCAGCACCT CAAGCAGCTT CTCTCTAACC AGGCCCTCAC CAAGAAGCTC 
TACAAACGCT ACGCCTTCCT GCGCTGTGAG GAGGAGAAAG AACAATTTCT GTATCATCTC 
CTCTCACTCA ATGCTGTGGA CTATTTCTGC TTCACCAGCG TCTTCACCAC TATCATGATC 
CCGTACAAAG CTGTGATAAT CCCCATCAAG AAACTAAGTA ACGTCATCAC TACATCAAAC 
CCCTGGATCT GTGTCTCGGG GGAGTTTGGT GATTCCGGAG TCATGCAGAT CCCCAAGAAC 
AACCTGGAGA TAAACTTTGA GTGCCAAAAT CTGGGTAAGA TGACAACAGT TCAGATCGGA 
CATGACAATT CCGGACTGTT AGCTAAGTGG CTGGTTGACT GCGTAATGGT CAGAAATGAG 
ATCACAGGAC ACACCTACAA GTTCCCTTGT GGTCGATGGC TGGGGAAAGG CGTGGACGAT 
GGGAGCTTGG AGCGAATTCT CATTGGCGAG CTGATAATGC CCATAGCAGA TGAAGACCTG 
GGGAAGCAGT GTAGGACTCC CCCTCAACAG AAGTCCCCTA CGATGGCACG ACGGCTGAGC 
ATTACATCAC TTGGAGGTGG AAAGCTGAAC AGACCCAATT CTGGAAAAAT CCAAGAAGGA 
ATAGGAGAAG CGGTGAACAA CATTGTGAAG CATTTCCATA AGCCAGAAAA AGAGGTAATT 
ACAACATCAT ATTGGTATGT GGATGCTAAA TGTTCACCTT ATGCCAAAAA AACTGTCGCC 
TACTTTGAAA CCACCGATCA GATTGGGGAT GCTGAGGAAG ACCTGCTACT ACAAAGATCT 
GCCTGCAAAA CCTTCTGCCA GTATGTTAAT GGCATTAACA CGGCACCAAG AAATTTTGGG 
AAGGATGTCA AATTCCAGCT GCTAGTTTGC CTTGGTGCAA GGGATCATTT GCTTCCTCAG 
TGGATCCTGT TGCTGTCGGA CTGCCCATCT ATCACGAGGA TGTATGAGGA GAACGCCCTA 
CTCCGAGACC AGCTGATGGT GAACTCCCTC ATGCGAGTCT TGCACACTTT GGAAGAATTT 
AACATCACCC TGGAAGCTTC ACTGGTCAAA GGAATTGACC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014347559.1
CDS	255..4079
Translation	MSVSAPGSGSAPCRFAHYFVICGIDTDSGLEPDELAGENFEQSPLKRTFKSKVLAHYPQNVEWNPFDQDAVNMLCMPKGLSFRTQMENRDPQFHSYIITREDGSRTYGFVLTFYEEVTCKQICTAMQTLYQMHNAEHYSSVCASSSCSMDSLASSLDEGDNTSLSKLQRCNSYDISRDTLYVSKCICLITPLPFMRACRKFLSQLHRAVTSQQPPPLPLESYIHNILYEVPLPPPGRSLKFYGVYEPIVCQRPGPSELPLSDYPLREVFELLGVENLVQVFSCVLLEMQILLYSQDYQRLMTVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHFIELPEDFPQFPNKLEFIQEISEVLLQFGIPPEGSLHCSESATKLKSLVLDDLADDKKNGNIPANVYELLKGNETIARLQALAKRTGVRVDKIEITRMLAEKEEKDSKLPCDWEELRDYKLNVQVREVFASRFTQMFADYEAFVILTAQDLDSWLTNREQMQNFDKASFLSDQPEPYLPFLSHFIETQMFATFIDNKIMSQWEEKDPLLRVLDSRIEKIRLYSVRAPALRTSMYQKCTVLKEAAQSIEQRLAKIDHTAIHPHLLDMKIGQGKYEQGFFPKLQSDVLATGPTNNNRWASRSATAQRRKDRLRQHSEHLGLDNDLREVRYLMHGGLTRRGLEGSGISTGTRTLSPLVSNGSLNILSSEGLLVQQNSMAFWEWDQGSPPPPARPPKKSFSECCLTKRMLVEKMGHEAVELGHGEANITGLEENTLIASLCDLLERIWSHGLQIKQGKSALWSHLHQYQEREEKQDPVLESPVSLGPERRKSESGITLPSLRVSLIHDMRHIQNMSEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQALTKKLYKRYAFLRCEEEKEQFLYHLLSLNAVDYFCFTSVFTTIMIPYKAVIIPIKKLSNVITTSNPWICVSGEFGDSGVMQIPKNNLEINFECQNLGKMTTVQIGHDNSGLLAKWLVDCVMVRNEITGHTYKFPCGRWLGKGVDDGSLERILIGELIMPIADEDLGKQCRTPPQQKSPTMARRLSITSLGGGKLNRPNSGKIQEGIGEAVNNIVKHFHKPEKEVITTSYWYVDAKCSPYAKKTVAYFETTDQIGDAEEDLLLQRSACKTFCQYVNGINTAPRNFGKDVKFQLLVCLGARDHLLPQWILLLSDCPSITRMYEENALLRDQLMVNSLMRVLHTLEEFNITLEASLVKGIDL

Target ORF information:

RefSeq Version	XM_014492073.1
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae DENN/MADD domain containing 5B (DENND5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014492073.1

ORF Insert Sequence:

ATGAGCGTGA GCGCCCCCGG CTCGGGATCG GCTCCCTGCC GCTTCGCTCA TTACTTCGTG 
ATCTGCGGCA TCGACACGGA CAGCGGGCTG GAGCCCGACG AGCTGGCCGG AGAAAACTTT 
GAGCAGAGCC CGCTGAAAAG AACTTTCAAG TCCAAAGTTT TGGCCCACTA CCCTCAGAAC 
GTGGAGTGGA ATCCTTTTGA CCAAGATGCT GTTAACATGC TCTGCATGCC CAAAGGCCTT 
TCCTTCCGAA CGCAGATGGA GAACAGAGAT CCTCAGTTCC ACTCCTACAT CATCACCCGT 
GAGGATGGCT CCCGCACCTA CGGCTTCGTC CTCACCTTCT ACGAGGAGGT GACTTGCAAG 
CAGATCTGCA CAGCCATGCA GACCCTGTAC CAGATGCACA ATGCTGAGCA CTACAGTAGC 
GTCTGCGCCT CCTCCTCGTG CAGTATGGAC TCGCTGGCCA GCAGCCTCGA CGAGGGGGAC 
AACACCTCCC TCTCCAAACT CCAGCGCTGC AACTCCTACG ACATCAGCAG GGACACGCTC 
TACGTCTCCA AGTGCATCTG CCTCATCACC CCTCTCCCTT TCATGCGGGC CTGCCGGAAG 
TTCCTCAGTC AACTTCACAG GGCCGTCACC TCCCAGCAGC CCCCTCCGTT GCCCCTGGAG 
AGCTACATCC ATAACATCCT GTACGAGGTG CCGCTGCCGC CTCCCGGCAG GTCGTTGAAG 
TTCTACGGGG TTTATGAACC CATTGTCTGC CAGCGACCAG GCCCCAGCGA GTTGCCTCTG 
TCCGACTACC CTCTCCGTGA AGTCTTTGAG CTGCTTGGGG TCGAAAACCT GGTGCAAGTC 
TTCAGCTGTG TACTGCTAGA GATGCAGATT CTTCTCTACT CGCAAGATTA CCAGCGGCTG 
ATGACAGTAG CAGAAGGGAT TACAACACTC TTGTTCCCCT TTCAGTGGCA GCATGTCTAC 
GTTCCTATCC TTCCTGCCTC CCTTCTCCAT TTCCTGGATG CACCTGTCCC CTATCTAATG 
GGTCTCCAGT CCAAAGAAGG AACTGACCGC TCCAAGCTAG AACTTCCACA AGAGGCTAAC 
TTATGTTTCG TAGACATCGA TAACCACTTC ATTGAGCTGC CGGAGGATTT TCCACAGTTC 
CCCAACAAGC TGGAATTCAT TCAGGAAATC TCGGAGGTCC TCCTTCAGTT TGGAATCCCC 
CCCGAGGGTA GCCTGCACTG CAGTGAGAGT GCCACCAAGC TGAAGAGCTT GGTCCTGGAT 
GACCTGGCAG ATGACAAGAA GAATGGCAAC ATCCCCGCCA ACGTGTATGA GCTTCTCAAA 
GGCAACGAAA CCATCGCCCG GCTCCAAGCC CTGGCTAAGC GAACGGGAGT GAGGGTGGAC 
AAGATTGAGA TCACCAGGAT GCTGGCTGAG AAGGAGGAGA AGGACTCCAA GCTGCCTTGT 
GACTGGGAAG AACTGCGGGA TTACAAGCTG AACGTGCAGG TCCGTGAGGT CTTTGCCAGC 
CGCTTCACGC AGATGTTTGC GGACTATGAG GCCTTTGTGA TCCTGACGGC GCAGGACTTG 
GACTCCTGGC TCACAAATCG AGAGCAGATG CAGAACTTTG ACAAAGCATC CTTTCTGTCC 
GACCAGCCTG AACCTTACCT CCCCTTCCTC TCGCACTTCA TCGAAACCCA GATGTTTGCC 
ACATTCATCG ACAATAAGAT CATGTCGCAG TGGGAGGAGA AGGACCCCTT ACTCAGAGTG 
CTTGACTCGC GCATTGAGAA GATAAGGCTT TACAGCGTCC GGGCACCTGC CCTTCGAACA 
TCCATGTATC AAAAATGCAC TGTGTTAAAA GAAGCAGCTC AGTCTATTGA GCAGCGGTTA 
GCAAAGATCG ATCACACTGC CATTCACCCA CACTTGCTTG ACATGAAGAT CGGACAAGGC 
AAGTACGAGC AAGGCTTCTT CCCCAAGCTG CAGTCTGACG TGCTGGCCAC GGGACCGACC 
AATAACAACC GTTGGGCCAG CCGAAGCGCC ACAGCACAGC GCCGTAAAGA CCGTCTCCGG 
CAGCACTCGG AGCACCTCGG GCTGGACAAT GATTTAAGAG AGGTACGGTA TCTAATGCAT 
GGAGGGTTGA CCAGAAGGGG ATTGGAGGGC TCAGGTATTA GCACTGGCAC GCGGACCCTT 
TCACCTCTAG TATCAAATGG TTCTCTTAAC ATCCTGTCAT CAGAGGGGCT TCTGGTCCAG 
CAGAATTCCA TGGCCTTCTG GGAGTGGGAT CAGGGGTCAC CACCCCCTCC TGCACGACCT 
CCTAAAAAGT CATTCTCTGA GTGCTGCCTG ACCAAAAGGA TGTTAGTGGA GAAGATGGGA 
CACGAGGCGG TGGAACTGGG ACACGGGGAG GCCAACATCA CCGGCCTGGA AGAGAACACC 
CTGATCGCCA GTCTGTGTGA CCTACTGGAG AGGATCTGGA GCCATGGCCT ACAGATCAAG 
CAGGGGAAGT CTGCTTTGTG GTCTCACCTC CATCAGTACC AGGAGAGAGA GGAGAAGCAA 
GATCCAGTAC TGGAATCTCC AGTGTCCCTT GGACCAGAGA GAAGAAAATC TGAATCGGGC 
ATTACGTTGC CTTCACTCAG GGTATCCCTT ATCCACGACA TGAGGCACAT CCAGAATATG 
AGTGAGATAA AGACAGATGT TGGTCGGGCT CGAGCTTGGA TCAGGCTGTC CCTGGAAAAG 
AAGCTCTTAT CTCAGCACCT CAAGCAGCTT CTCTCTAACC AGGCCCTCAC CAAGAAGCTC 
TACAAACGCT ACGCCTTCCT GCGCTGTGAG GAGGAGAAAG AACAATTTCT GTATCATCTC 
CTCTCACTCA ATGCTGTGGA CTATTTCTGC TTCACCAGCG TCTTCACCAC TATCATGATC 
CCGTACAAAG CTGTGATAAT CCCCATCAAG AAACTAAGTA ACGTCATCAC TACATCAAAC 
CCCTGGATCT GTGTCTCGGG GGAGTTTGGT GATTCCGGAG TCATGCAGAT CCCCAAGAAC 
AACCTGGAGA TAAACTTTGA GTGCCAAAAT CTGGGTAAGA TGACAACAGT TCAGATCGGA 
CATGACAATT CCGGACTGTT AGCTAAGTGG CTGGTTGACT GCGTAATGGT CAGAAATGAG 
ATCACAGGAC ACACCTACAA GTTCCCTTGT GGTCGATGGC TGGGGAAAGG CGTGGACGAT 
GGGAGCTTGG AGCGAATTCT CATTGGCGAG CTGATAATGC CCATAGCAGA TGAAGACCTG 
GGGAAGCAGT GTAGGACTCC CCCTCAACAG AAGTCCCCTA CGATGGCACG ACGGCTGAGC 
ATTACATCAC TTGGAGGTGG AAAGCTGAAC AGACCCAATT CTGGAAAAAT CCAAGAAGGA 
ATAGGAGAAG CGGTGAACAA CATTGTGAAG CATTTCCATA AGCCAGAAAA AGAGGTAATT 
ACAACATCAT ATTGGTATGT GGATGCTAAA TGTTCACCTT ATGCCAAAAA AACTGTCGCC 
TACTTTGAAA CCACCGATCA GATTGGGGAT GCTGAGGAAG ACCTGCTACT ACAAAGATCT 
GCCTGCAAAA CCTTCTGCCA GTATGTTAAT GGCATTAACA CGGCACCAAG AAATTTTGGG 
AAGGATGTCA AATTCCAGCT GCTAGTTTGC CTTGGTGCAA GGGATCATTT GCTTCCTCAG 
TGGATCCTGT TGCTGTCGGA CTGCCCATCT ATCACGAGGA TGTATGAGGA GAACGCCCTA 
CTCCGAGACC AGCTGATGGT GAACTCCCTC ATGCGAGTCT TGCACACTTT GGAAGAATTT 
AACATCACCC TGGAAGCTTC ACTGGTCAAA GGAATTGACC TGTAG