dbt cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	dbt
Entrez Gene ID	104936350
Full Name	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Synonyms	dbt
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010752374.2	XP_010750676.1	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
XM_010752374.3	XP_010750676.1	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial

Pathways from BioSystems
Gene Ontology

The following dbt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dbt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa111232	XM_010752374.3 Latest version!	Larimichthys crocea dihydrolipoamide branched chain transacylase E2 (dbt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OLa111232	XM_010752374.2	Larimichthys crocea lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (LOC104936350), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dbt ( XM_010752374.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010750676.1 Larimichthys crocea lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (LOC104936350), mRNA.

Price & Availability↑

CloneID	OLa111232
Clone ID Related Accession (Same CDS sequence)	XM_010752374.2 , XM_010752374.3
Accession Version	XM_010752374.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609529.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010752374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG TGACCAGAGG CTCGTTGTCC GCGCTCCGAC AGCTGCTGAG TCAGCAGTAC 
CGCCGCCGCT GTTTCCCACC TCAGAACCTG AGGTCCAACA GAACCCTGCA GCCGCTCGCC 
TTCAGATGTG ACCGACACCT CCAGATGTTG AGCAGCAGGA CGCTGCACAC CGCCGCTGTG 
AGTCGAGGTC CGGTCCTCCA GTTCAAACTG TCCGACATTG GAGAGGGCAT CATGGAGGTG 
ACGGTGAAGG AGTGGTATGT GAAGGAGGGG GACAAGGTGT CTCAGTTCGA CAGTATCTGT 
GAGGTCCAGA GTGACAAGGC GTCGGTTACC ATCACCAGCC GCTACGACGG AGTCATTAAG 
AAACTCTACT ACGACGTGGA CGCCACCGCC TTGGTGGGCA AGCCACTGGT GGACATTGAG 
ACAGAGTCCA GTTCAGAGGT GATCCAGGAG GAAGACGTGG TGGAGACGCC CGCCATGGCT 
CGAGAAGAAC ACACCCACCA GGAGATCAAA GGTCACAAGA CCCAGGCCAC GCCTGCAGTC 
AGACGCCTCG CCATGGAGAA CAACATCAAG CTCAGCGAGG TGGTGGGGAC GGGTAAAGAT 
GGACGGATCC TGAAGGAGGA CATCTTAAAC TATCTGGCGA AGCAGACAGG AGCCATCTTG 
CCTCCGACTC CATTCCAGGA GATCCAGACT CCGCCTCCGC CCCCAGCTGC CAGGCCCATG 
AGCACTCCGG CAGCCATCAA AGCTCCACCC ACAATGCCCA AACCGGTGTT CACCGGCAAA 
GACGTGACCG AACCCCTCAA AGGTTTCCAC CGAGCGATGG TGAAGACAAT GACAGCGGCG 
CTGAAGATTC CTCACTTTGG GTACTGTGAC GAGGTGGACT TGAGCCGGCT GGTGTCTCTG 
AGAGCTGAGC TCAAATCCAT CACTGAAGGT CGTGGGGTCA AACTCAGCTA CATGCCGTTC 
TTCATCAAGG CTGCCTCCCT CGGCCTCCTG CACTTTCCAA TCCTCAACGC CTCGGTGGAC 
GAAAGCTGCC AGAACATCAC GTACAAGGCT TCTCATAACA TCGGCCTGGC GATGGACACC 
AGTCAGGGTC TGCTGGTTCC CAACGTGAAG AACGTTCAGC TGCTCAGCGT GTTCGAGATC 
GCTCAGGAGC TGAACCGCCT GCAGGCGCTC GGAGCTGCAG GTCAGCTGGG AACCAACGAG 
CTCAGCGGGG GAACCTTCAC CCTGTCCAAC ATTGGATCAA TCGGAGGGAC GTATGCTAAG 
CCGGTCATTC TTCCTCCTGA AGTCGCCATC GGAGCGCTGG GAAAAATCCA GGTTCTTCCT 
CGCTTCGACG CCGGCGGTCA GGTGGTCCGA GCTCACATCA TGAACGTCAG CTGGTCTGCA 
GATCATCGCA TCATCGACGG CGCCACCATG TGTCGTTTCT CCAACCTGTG GAGGGAGTAC 
CTGGAGAACC CGGCCAGCAT GGTGCTGGAC CTGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010750676.1
CDS	64..1542
Translation	MAAVTRGSLSALRQLLSQQYRRRCFPPQNLRSNRTLQPLAFRCDRHLQMLSSRTLHTAAVSRGPVLQFKLSDIGEGIMEVTVKEWYVKEGDKVSQFDSICEVQSDKASVTITSRYDGVIKKLYYDVDATALVGKPLVDIETESSSEVIQEEDVVETPAMAREEHTHQEIKGHKTQATPAVRRLAMENNIKLSEVVGTGKDGRILKEDILNYLAKQTGAILPPTPFQEIQTPPPPPAARPMSTPAAIKAPPTMPKPVFTGKDVTEPLKGFHRAMVKTMTAALKIPHFGYCDEVDLSRLVSLRAELKSITEGRGVKLSYMPFFIKAASLGLLHFPILNASVDESCQNITYKASHNIGLAMDTSQGLLVPNVKNVQLLSVFEIAQELNRLQALGAAGQLGTNELSGGTFTLSNIGSIGGTYAKPVILPPEVAIGALGKIQVLPRFDAGGQVVRAHIMNVSWSADHRIIDGATMCRFSNLWREYLENPASMVLDLK

Target ORF information:

RefSeq Version	XM_010752374.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (LOC104936350), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010752374.2

ORF Insert Sequence:

ATGGCGGCGG TGACCAGAGG CTCGTTGTCC GCGCTCCGAC AGCTGCTGAG TCAGCAGTAC 
CGCCGCCGCT GTTTCCCACC TCAGAACCTG AGGTCCAACA GAACCCTGCA GCCGCTCGCC 
TTCAGATGTG ACCGACACCT CCAGATGTTG AGCAGCAGGA CGCTGCACAC CGCCGCTGTG 
AGTCGAGGTC CGGTCCTCCA GTTCAAACTG TCCGACATTG GAGAGGGCAT CATGGAGGTG 
ACGGTGAAGG AGTGGTATGT GAAGGAGGGG GACAAGGTGT CTCAGTTCGA CAGTATCTGT 
GAGGTCCAGA GTGACAAGGC GTCGGTTACC ATCACCAGCC GCTACGACGG AGTCATTAAG 
AAACTCTACT ACGACGTGGA CGCCACCGCC TTGGTGGGCA AGCCACTGGT GGACATTGAG 
ACAGAGTCCA GTTCAGAGGT GATCCAGGAG GAAGACGTGG TGGAGACGCC CGCCATGGCT 
CGAGAAGAAC ACACCCACCA GGAGATCAAA GGTCACAAGA CCCAGGCCAC GCCTGCAGTC 
AGACGCCTCG CCATGGAGAA CAACATCAAG CTCAGCGAGG TGGTGGGGAC GGGTAAAGAT 
GGACGGATCC TGAAGGAGGA CATCTTAAAC TATCTGGCGA AGCAGACAGG AGCCATCTTG 
CCTCCGACTC CATTCCAGGA GATCCAGACT CCGCCTCCGC CCCCAGCTGC CAGGCCCATG 
AGCACTCCGG CAGCCATCAA AGCTCCACCC ACAATGCCCA AACCGGTGTT CACCGGCAAA 
GACGTGACCG AACCCCTCAA AGGTTTCCAC CGAGCGATGG TGAAGACAAT GACAGCGGCG 
CTGAAGATTC CTCACTTTGG GTACTGTGAC GAGGTGGACT TGAGCCGGCT GGTGTCTCTG 
AGAGCTGAGC TCAAATCCAT CACTGAAGGT CGTGGGGTCA AACTCAGCTA CATGCCGTTC 
TTCATCAAGG CTGCCTCCCT CGGCCTCCTG CACTTTCCAA TCCTCAACGC CTCGGTGGAC 
GAAAGCTGCC AGAACATCAC GTACAAGGCT TCTCATAACA TCGGCCTGGC GATGGACACC 
AGTCAGGGTC TGCTGGTTCC CAACGTGAAG AACGTTCAGC TGCTCAGCGT GTTCGAGATC 
GCTCAGGAGC TGAACCGCCT GCAGGCGCTC GGAGCTGCAG GTCAGCTGGG AACCAACGAG 
CTCAGCGGGG GAACCTTCAC CCTGTCCAAC ATTGGATCAA TCGGAGGGAC GTATGCTAAG 
CCGGTCATTC TTCCTCCTGA AGTCGCCATC GGAGCGCTGG GAAAAATCCA GGTTCTTCCT 
CGCTTCGACG CCGGCGGTCA GGTGGTCCGA GCTCACATCA TGAACGTCAG CTGGTCTGCA 
GATCATCGCA TCATCGACGG CGCCACCATG TGTCGTTTCT CCAACCTGTG GAGGGAGTAC 
CTGGAGAACC CGGCCAGCAT GGTGCTGGAC CTGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dbt ( XM_010752374.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010750676.1 Larimichthys crocea dihydrolipoamide branched chain transacylase E2 (dbt), mRNA.

Price & Availability↑

CloneID	OLa111232
Clone ID Related Accession (Same CDS sequence)	XM_010752374.2 , XM_010752374.3
Accession Version	XM_010752374.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040024.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010752374.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG TGACCAGAGG CTCGTTGTCC GCGCTCCGAC AGCTGCTGAG TCAGCAGTAC 
CGCCGCCGCT GTTTCCCACC TCAGAACCTG AGGTCCAACA GAACCCTGCA GCCGCTCGCC 
TTCAGATGTG ACCGACACCT CCAGATGTTG AGCAGCAGGA CGCTGCACAC CGCCGCTGTG 
AGTCGAGGTC CGGTCCTCCA GTTCAAACTG TCCGACATTG GAGAGGGCAT CATGGAGGTG 
ACGGTGAAGG AGTGGTATGT GAAGGAGGGG GACAAGGTGT CTCAGTTCGA CAGTATCTGT 
GAGGTCCAGA GTGACAAGGC GTCGGTTACC ATCACCAGCC GCTACGACGG AGTCATTAAG 
AAACTCTACT ACGACGTGGA CGCCACCGCC TTGGTGGGCA AGCCACTGGT GGACATTGAG 
ACAGAGTCCA GTTCAGAGGT GATCCAGGAG GAAGACGTGG TGGAGACGCC CGCCATGGCT 
CGAGAAGAAC ACACCCACCA GGAGATCAAA GGTCACAAGA CCCAGGCCAC GCCTGCAGTC 
AGACGCCTCG CCATGGAGAA CAACATCAAG CTCAGCGAGG TGGTGGGGAC GGGTAAAGAT 
GGACGGATCC TGAAGGAGGA CATCTTAAAC TATCTGGCGA AGCAGACAGG AGCCATCTTG 
CCTCCGACTC CATTCCAGGA GATCCAGACT CCGCCTCCGC CCCCAGCTGC CAGGCCCATG 
AGCACTCCGG CAGCCATCAA AGCTCCACCC ACAATGCCCA AACCGGTGTT CACCGGCAAA 
GACGTGACCG AACCCCTCAA AGGTTTCCAC CGAGCGATGG TGAAGACAAT GACAGCGGCG 
CTGAAGATTC CTCACTTTGG GTACTGTGAC GAGGTGGACT TGAGCCGGCT GGTGTCTCTG 
AGAGCTGAGC TCAAATCCAT CACTGAAGGT CGTGGGGTCA AACTCAGCTA CATGCCGTTC 
TTCATCAAGG CTGCCTCCCT CGGCCTCCTG CACTTTCCAA TCCTCAACGC CTCGGTGGAC 
GAAAGCTGCC AGAACATCAC GTACAAGGCT TCTCATAACA TCGGCCTGGC GATGGACACC 
AGTCAGGGTC TGCTGGTTCC CAACGTGAAG AACGTTCAGC TGCTCAGCGT GTTCGAGATC 
GCTCAGGAGC TGAACCGCCT GCAGGCGCTC GGAGCTGCAG GTCAGCTGGG AACCAACGAG 
CTCAGCGGGG GAACCTTCAC CCTGTCCAAC ATTGGATCAA TCGGAGGGAC GTATGCTAAG 
CCGGTCATTC TTCCTCCTGA AGTCGCCATC GGAGCGCTGG GAAAAATCCA GGTTCTTCCT 
CGCTTCGACG CCGGCGGTCA GGTGGTCCGA GCTCACATCA TGAACGTCAG CTGGTCTGCA 
GATCATCGCA TCATCGACGG CGCCACCATG TGTCGTTTCT CCAACCTGTG GAGGGAGTAC 
CTGGAGAACC CGGCCAGCAT GGTGCTGGAC CTGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010750676.1
CDS	70..1548
Translation	MAAVTRGSLSALRQLLSQQYRRRCFPPQNLRSNRTLQPLAFRCDRHLQMLSSRTLHTAAVSRGPVLQFKLSDIGEGIMEVTVKEWYVKEGDKVSQFDSICEVQSDKASVTITSRYDGVIKKLYYDVDATALVGKPLVDIETESSSEVIQEEDVVETPAMAREEHTHQEIKGHKTQATPAVRRLAMENNIKLSEVVGTGKDGRILKEDILNYLAKQTGAILPPTPFQEIQTPPPPPAARPMSTPAAIKAPPTMPKPVFTGKDVTEPLKGFHRAMVKTMTAALKIPHFGYCDEVDLSRLVSLRAELKSITEGRGVKLSYMPFFIKAASLGLLHFPILNASVDESCQNITYKASHNIGLAMDTSQGLLVPNVKNVQLLSVFEIAQELNRLQALGAAGQLGTNELSGGTFTLSNIGSIGGTYAKPVILPPEVAIGALGKIQVLPRFDAGGQVVRAHIMNVSWSADHRIIDGATMCRFSNLWREYLENPASMVLDLK

Target ORF information:

RefSeq Version	XM_010752374.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea dihydrolipoamide branched chain transacylase E2 (dbt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010752374.3

ORF Insert Sequence:

ATGGCGGCGG TGACCAGAGG CTCGTTGTCC GCGCTCCGAC AGCTGCTGAG TCAGCAGTAC 
CGCCGCCGCT GTTTCCCACC TCAGAACCTG AGGTCCAACA GAACCCTGCA GCCGCTCGCC 
TTCAGATGTG ACCGACACCT CCAGATGTTG AGCAGCAGGA CGCTGCACAC CGCCGCTGTG 
AGTCGAGGTC CGGTCCTCCA GTTCAAACTG TCCGACATTG GAGAGGGCAT CATGGAGGTG 
ACGGTGAAGG AGTGGTATGT GAAGGAGGGG GACAAGGTGT CTCAGTTCGA CAGTATCTGT 
GAGGTCCAGA GTGACAAGGC GTCGGTTACC ATCACCAGCC GCTACGACGG AGTCATTAAG 
AAACTCTACT ACGACGTGGA CGCCACCGCC TTGGTGGGCA AGCCACTGGT GGACATTGAG 
ACAGAGTCCA GTTCAGAGGT GATCCAGGAG GAAGACGTGG TGGAGACGCC CGCCATGGCT 
CGAGAAGAAC ACACCCACCA GGAGATCAAA GGTCACAAGA CCCAGGCCAC GCCTGCAGTC 
AGACGCCTCG CCATGGAGAA CAACATCAAG CTCAGCGAGG TGGTGGGGAC GGGTAAAGAT 
GGACGGATCC TGAAGGAGGA CATCTTAAAC TATCTGGCGA AGCAGACAGG AGCCATCTTG 
CCTCCGACTC CATTCCAGGA GATCCAGACT CCGCCTCCGC CCCCAGCTGC CAGGCCCATG 
AGCACTCCGG CAGCCATCAA AGCTCCACCC ACAATGCCCA AACCGGTGTT CACCGGCAAA 
GACGTGACCG AACCCCTCAA AGGTTTCCAC CGAGCGATGG TGAAGACAAT GACAGCGGCG 
CTGAAGATTC CTCACTTTGG GTACTGTGAC GAGGTGGACT TGAGCCGGCT GGTGTCTCTG 
AGAGCTGAGC TCAAATCCAT CACTGAAGGT CGTGGGGTCA AACTCAGCTA CATGCCGTTC 
TTCATCAAGG CTGCCTCCCT CGGCCTCCTG CACTTTCCAA TCCTCAACGC CTCGGTGGAC 
GAAAGCTGCC AGAACATCAC GTACAAGGCT TCTCATAACA TCGGCCTGGC GATGGACACC 
AGTCAGGGTC TGCTGGTTCC CAACGTGAAG AACGTTCAGC TGCTCAGCGT GTTCGAGATC 
GCTCAGGAGC TGAACCGCCT GCAGGCGCTC GGAGCTGCAG GTCAGCTGGG AACCAACGAG 
CTCAGCGGGG GAACCTTCAC CCTGTCCAAC ATTGGATCAA TCGGAGGGAC GTATGCTAAG 
CCGGTCATTC TTCCTCCTGA AGTCGCCATC GGAGCGCTGG GAAAAATCCA GGTTCTTCCT 
CGCTTCGACG CCGGCGGTCA GGTGGTCCGA GCTCACATCA TGAACGTCAG CTGGTCTGCA 
GATCATCGCA TCATCGACGG CGCCACCATG TGTCGTTTCT CCAACCTGTG GAGGGAGTAC 
CTGGAGAACC CGGCCAGCAT GGTGCTGGAC CTGAAATAG