LOC104934208 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104934208
Entrez Gene ID	104934208
Full Name	collagen type VI alpha 1 chain
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010749821.2	XP_010748123.2	collagen alpha-1(VI) chain
XM_027283285.1	XP_027139086.1	collagen alpha-1(VI) chain

Pathways from BioSystems
Gene Ontology

The following LOC104934208 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104934208 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa198837	XM_027283285.1 Latest version!	Larimichthys crocea collagen alpha-1(VI) chain (LOC104934208), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OLa87475	XM_010749821.2 Latest version!	Larimichthys crocea collagen type VI alpha 1 chain (col6a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104934208 ( XM_027283285.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027139086.1 Larimichthys crocea collagen alpha-1(VI) chain (LOC104934208), mRNA.

Price & Availability↑

CloneID	OLa198837
Clone ID Related Accession (Same CDS sequence)	XM_027283285.1
Accession Version	XM_027283285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	collagen alpha-1(VI) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAAAA ACGAGACAAG GGATACGTGT TGCTCCTTCG AATGCACTGC TCCAGGAGGG 
CCAAAGGGAT TGATAGGAGA CCCTGGATCC AAGGGAGAGT CAGGTAGACC AGGAATGCCA 
GGAGAGAAAG GAGATCCTGG TCCCAAGGGC AGCATTGGAG ATCCTGGTCC AGTTGGTTAC 
AGTGGAATGA AGGGAGACCG TGGTGGCAGA GGAGAAAAGG GTGAAAGAGG ACACAAAGGC 
TTCAAGGGAG ACAAAGGTCA AAGGGGAAGG GATGGAATTG ATGGACGCAA GGGTGAGGCT 
GGTTTCGCTG GTCTTCCCGG CTGTAAAGGA TCTCCCGGAT CAGATGGCCT ACAGGGAGAT 
CCTGGACCAA AGGGAGACCC TGGTCCTTAT GGAATAAAAG GAGAAAAAGG AGATCCTGGA 
AAAGACGGCC TGTCTGGAAG ACCTGGAAAC TATGGTGCAT CGGGACCTAA GGGCGAGCCG 
GGCTCTCGTG GAGGCGATGG GGACAAAGGA GAGCGCGGTG ACGATGGCCC TACAGGACCA 
GATGGACCTC GCGGCGAGAG AGGAACTATT GGAGAGAAAG GGGTTCAAGG TTCCCCCGGA 
AACAGAGGTC CAAGAGGAGA TCCCGGGGAC CCTGGACCCC GTGGAGAACC GGGGAGGGAG 
GGCACACCTG GTCCCAACGG AGACCCTGGT GTGAAAGGCA GAGAAGGGCC TCCTGGCTAC 
AGAGGAGATG AAGGACCATC TGGACCAGAA GGACCCAAAG GAATCAGAGG AATCAAAGGC 
GCTCCTGGAG ACAGAGGCCT GATTGGCGAC AGGGGAGAAG ATGGTGCCCC AGGAAATGGT 
ACCGCAGGTT GTCCTGGCTT CCAGGGTTAT CCTGGGACCC GTGGAGATCC TGGTGAGCCT 
GGTGGCAAGG GAACCCCTGG ACCCAAAGGA GATGACGGAG ATCCCGGAGA TCCAGGCCCC 
GATAACGACA TCCCAGGAAC TCCAGGACGT AAAGGAGCCA AAGGTCACAG AGGACCCGAC 
GGCAATCCAG GACCTCCTGG GCCTCCTGGA CCAGCAGGAA CTGATGAATG TGAAATTCTG 
GATATCATCA TGAAGCTCTG TTCTTGTTGT GAGTGTAAGT GTGGATCTCT GGACCTGGCG 
TTCATTGTGG ACAGCTCAGA GAGTATTGGT GAATCAAACT TTGCTCTTGC CAAAGACTTT 
ATCATCACAG TCATTGACAG ACTGATCAAA GACCAGCAAG TCAAGTTTGC AGGTAATGAG 
TCCAGAGTGA GTGTAGTGCA GTACAGTGGA GCAAGGGCCC AAGAAGCTGT CCAATTGGGA 
TCCAACGCTT TTAGCCTCAC CGACTTCAAA CAGAAAGTGA GAGACTTGAG ATGGCTGGCT 
GAGGCCACAT ACACAGGCGA GGCCCTTGAT TTCGCTCTAA AAAACACAAT CAGTCTGATG 
CGGCAAGACA ACCGAGTTGT TCTTGTCCTC ACCGACGGAC GTTCTGATAC TGATAGGGAC 
ACGATTCCCC TCAACATCCT CTGTGGTCAA AATGTCCGGG TGGGTGGTTT AGGAGTGAAG 
GACTACGCAG GTCGTCAGCC CAACCAGGAG CGGCTTGATG AAATGAGTTG TAAGAACGAT 
CCCAAACCAG GCTTTTCCTT TGTTCTGGAC AACTTTGCCG AGCTGCTGGA TGACACTTTC 
CTGCAAAACC TCACAACCAA AATCTGTCAA GATAAGAAGT GCCCGTCGGC ACGACAAAGA 
TGTCCCATTT CTTTCGATGA AAGTGCCGAT ATTTTGGTGA TGATGGACAG CTCGGCGAGT 
GTGGGCCAGA AGAACTTCGA GACAAGCAAG ACCTTTGTCC GACGTTTGGC TGAGCGTTTC 
CTGACCGCAG AGAAAAAGAG GGTCGCCACT GTCAAAGTGG CCGTTGGCCA ATACAGCCGG 
AGCAGCCGCA TGGAGCAGCA GCTAACTACC AATTTGACCC TGCTGTCTCG TCGCATTGAA 
GAGGCAGCCT TCCAAAATGA CGGCACTAAT TTATTAGAGG CCATGGAGTT TGCTATTAAG 
AATTTACGTG GCCGTGGAGA CGCATCGGGA GGTGTAAAGA AGCTGGTGCT GTTCTCTGAC 
GGCAGGTCTC AGGATATCAC TGATGCTGGG CTGGAGAAGC GCGTACGTGA AGTGGCGGAA 
GCCGGGGTGG AGCTCTTTGT CATCGCCATT GGCACCCAGG TCAATGAGGC CAACTTGCGC 
ACTCTTGTGA GCAAAGGGCG TCGGGAAGAC ATCATCTCTG CCCAGCGCCA CCTGTTCCGC 
ATCGGAGACT ACCCCTCTCT GCTCAGAGGG GTCTTCTACC AAACCGTTTC CCGTAGGGTG 
TCCAAGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027139086.1
CDS	136..2487
Translation	MIKNETRDTCCSFECTAPGGPKGLIGDPGSKGESGRPGMPGEKGDPGPKGSIGDPGPVGYSGMKGDRGGRGEKGERGHKGFKGDKGQRGRDGIDGRKGEAGFAGLPGCKGSPGSDGLQGDPGPKGDPGPYGIKGEKGDPGKDGLSGRPGNYGASGPKGEPGSRGGDGDKGERGDDGPTGPDGPRGERGTIGEKGVQGSPGNRGPRGDPGDPGPRGEPGREGTPGPNGDPGVKGREGPPGYRGDEGPSGPEGPKGIRGIKGAPGDRGLIGDRGEDGAPGNGTAGCPGFQGYPGTRGDPGEPGGKGTPGPKGDDGDPGDPGPDNDIPGTPGRKGAKGHRGPDGNPGPPGPPGPAGTDECEILDIIMKLCSCCECKCGSLDLAFIVDSSESIGESNFALAKDFIITVIDRLIKDQQVKFAGNESRVSVVQYSGARAQEAVQLGSNAFSLTDFKQKVRDLRWLAEATYTGEALDFALKNTISLMRQDNRVVLVLTDGRSDTDRDTIPLNILCGQNVRVGGLGVKDYAGRQPNQERLDEMSCKNDPKPGFSFVLDNFAELLDDTFLQNLTTKICQDKKCPSARQRCPISFDESADILVMMDSSASVGQKNFETSKTFVRRLAERFLTAEKKRVATVKVAVGQYSRSSRMEQQLTTNLTLLSRRIEEAAFQNDGTNLLEAMEFAIKNLRGRGDASGGVKKLVLFSDGRSQDITDAGLEKRVREVAEAGVELFVIAIGTQVNEANLRTLVSKGRREDIISAQRHLFRIGDYPSLLRGVFYQTVSRRVSKS

Target ORF information:

RefSeq Version	XM_027283285.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea collagen alpha-1(VI) chain (LOC104934208), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027283285.1

ORF Insert Sequence:

ATGATTAAAA ACGAGACAAG GGATACGTGT TGCTCCTTCG AATGCACTGC TCCAGGAGGG 
CCAAAGGGAT TGATAGGAGA CCCTGGATCC AAGGGAGAGT CAGGTAGACC AGGAATGCCA 
GGAGAGAAAG GAGATCCTGG TCCCAAGGGC AGCATTGGAG ATCCTGGTCC AGTTGGTTAC 
AGTGGAATGA AGGGAGACCG TGGTGGCAGA GGAGAAAAGG GTGAAAGAGG ACACAAAGGC 
TTCAAGGGAG ACAAAGGTCA AAGGGGAAGG GATGGAATTG ATGGACGCAA GGGTGAGGCT 
GGTTTCGCTG GTCTTCCCGG CTGTAAAGGA TCTCCCGGAT CAGATGGCCT ACAGGGAGAT 
CCTGGACCAA AGGGAGACCC TGGTCCTTAT GGAATAAAAG GAGAAAAAGG AGATCCTGGA 
AAAGACGGCC TGTCTGGAAG ACCTGGAAAC TATGGTGCAT CGGGACCTAA GGGCGAGCCG 
GGCTCTCGTG GAGGCGATGG GGACAAAGGA GAGCGCGGTG ACGATGGCCC TACAGGACCA 
GATGGACCTC GCGGCGAGAG AGGAACTATT GGAGAGAAAG GGGTTCAAGG TTCCCCCGGA 
AACAGAGGTC CAAGAGGAGA TCCCGGGGAC CCTGGACCCC GTGGAGAACC GGGGAGGGAG 
GGCACACCTG GTCCCAACGG AGACCCTGGT GTGAAAGGCA GAGAAGGGCC TCCTGGCTAC 
AGAGGAGATG AAGGACCATC TGGACCAGAA GGACCCAAAG GAATCAGAGG AATCAAAGGC 
GCTCCTGGAG ACAGAGGCCT GATTGGCGAC AGGGGAGAAG ATGGTGCCCC AGGAAATGGT 
ACCGCAGGTT GTCCTGGCTT CCAGGGTTAT CCTGGGACCC GTGGAGATCC TGGTGAGCCT 
GGTGGCAAGG GAACCCCTGG ACCCAAAGGA GATGACGGAG ATCCCGGAGA TCCAGGCCCC 
GATAACGACA TCCCAGGAAC TCCAGGACGT AAAGGAGCCA AAGGTCACAG AGGACCCGAC 
GGCAATCCAG GACCTCCTGG GCCTCCTGGA CCAGCAGGAA CTGATGAATG TGAAATTCTG 
GATATCATCA TGAAGCTCTG TTCTTGTTGT GAGTGTAAGT GTGGATCTCT GGACCTGGCG 
TTCATTGTGG ACAGCTCAGA GAGTATTGGT GAATCAAACT TTGCTCTTGC CAAAGACTTT 
ATCATCACAG TCATTGACAG ACTGATCAAA GACCAGCAAG TCAAGTTTGC AGGTAATGAG 
TCCAGAGTGA GTGTAGTGCA GTACAGTGGA GCAAGGGCCC AAGAAGCTGT CCAATTGGGA 
TCCAACGCTT TTAGCCTCAC CGACTTCAAA CAGAAAGTGA GAGACTTGAG ATGGCTGGCT 
GAGGCCACAT ACACAGGCGA GGCCCTTGAT TTCGCTCTAA AAAACACAAT CAGTCTGATG 
CGGCAAGACA ACCGAGTTGT TCTTGTCCTC ACCGACGGAC GTTCTGATAC TGATAGGGAC 
ACGATTCCCC TCAACATCCT CTGTGGTCAA AATGTCCGGG TGGGTGGTTT AGGAGTGAAG 
GACTACGCAG GTCGTCAGCC CAACCAGGAG CGGCTTGATG AAATGAGTTG TAAGAACGAT 
CCCAAACCAG GCTTTTCCTT TGTTCTGGAC AACTTTGCCG AGCTGCTGGA TGACACTTTC 
CTGCAAAACC TCACAACCAA AATCTGTCAA GATAAGAAGT GCCCGTCGGC ACGACAAAGA 
TGTCCCATTT CTTTCGATGA AAGTGCCGAT ATTTTGGTGA TGATGGACAG CTCGGCGAGT 
GTGGGCCAGA AGAACTTCGA GACAAGCAAG ACCTTTGTCC GACGTTTGGC TGAGCGTTTC 
CTGACCGCAG AGAAAAAGAG GGTCGCCACT GTCAAAGTGG CCGTTGGCCA ATACAGCCGG 
AGCAGCCGCA TGGAGCAGCA GCTAACTACC AATTTGACCC TGCTGTCTCG TCGCATTGAA 
GAGGCAGCCT TCCAAAATGA CGGCACTAAT TTATTAGAGG CCATGGAGTT TGCTATTAAG 
AATTTACGTG GCCGTGGAGA CGCATCGGGA GGTGTAAAGA AGCTGGTGCT GTTCTCTGAC 
GGCAGGTCTC AGGATATCAC TGATGCTGGG CTGGAGAAGC GCGTACGTGA AGTGGCGGAA 
GCCGGGGTGG AGCTCTTTGT CATCGCCATT GGCACCCAGG TCAATGAGGC CAACTTGCGC 
ACTCTTGTGA GCAAAGGGCG TCGGGAAGAC ATCATCTCTG CCCAGCGCCA CCTGTTCCGC 
ATCGGAGACT ACCCCTCTCT GCTCAGAGGG GTCTTCTACC AAACCGTTTC CCGTAGGGTG 
TCCAAGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104934208 ( XM_010749821.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010748123.2 Larimichthys crocea collagen type VI alpha 1 chain (col6a1), mRNA.

Price & Availability↑

CloneID	OLa87475
Clone ID Related Accession (Same CDS sequence)	XM_010749821.2
Accession Version	XM_010749821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	collagen alpha-1(VI) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010749821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACTT TCAGAGGTCT CCTTGCGCTG CTGTGCCTTT TCCTGACTGA GGCGCAAACG 
CAGGACCGGA AAAACAGATT TGCGGATTGT CCAGTTGACC TCTTCTTTGT TCTGGATACA 
TCTGAGAGTG TCGCCCTACG ACAGCAACAT CCCAACTATT ATATTGACCA GATCAAAGCA 
TTCACCAATC AATTCATAGA TGAACTCCAG AACATGCGTC ATGTATGTGA GCGCATCCTG 
ACATGGAACT CAGGAGCACT CCACTACAGT GACGAAGTCG AAGTTGTAAA AGAGCTGAGT 
GACCTGTCGA CTGAGCGCGA GGCCCTGAAG AAAAGCGTCA GAGACATTCA ATACATCGGC 
AAGGGCACAT ATACTGACTG TGCCATCAAG AGAGGTCTTG CTGAGCTGCT TATTGGGGGC 
TCCAGCTACC AGGAGAACAA GTACATCGTG GTGGTGACTG ATGGTCACCC TATCACTGGT 
TACAAAGAGC CATGTGGAGG TGTGCAGGAG GCTGCTAATG AGGCCAGGCA ACATGGAGTC 
AAAGTGTTTG CTGTCGCCAT TTCACCTGAC CAGGAGGACA CCAGGCTGTC ACTCATTGCC 
ACAGACCACA CCTACAGACA GAACTTCACT GCTGTAGATG ACACCAGAGC CACCAAGATC 
GGAACCATTA GAACCATCAT CAATATGATT AAAAACGAGA CAAGGGATAC GTGTTGCTCC 
TTCGAATGCA CTGCTCCAGG AGGGCCAAAG GGATTGATAG GAGACCCTGG ATCCAAGGGA 
GAGTCAGGTA GACCAGGAAT GCCAGGAGAG AAAGGAGATC CTGGTCCCAA GGGCAGCATT 
GGAGATCCTG GTCCAGTTGG TTACAGTGGA ATGAAGGGAG ACCGTGGTGG CAGAGGAGAA 
AAGGGTGAAA GAGGACACAA AGGCTTCAAG GGAGACAAAG GTCAAAGGGG AAGGGATGGA 
ATTGATGGAC GCAAGGGTGA GGCTGGTTTC GCTGGTCTTC CCGGCTGTAA AGGATCTCCC 
GGATCAGATG GCCTACAGGG AGATCCTGGA CCAAAGGGAG ACCCTGGTCC TTATGGAATA 
AAAGGAGAAA AAGGAGATCC TGGAAAAGAC GGCCTGTCTG GAAGACCTGG AAACTATGGT 
GCATCGGGAC CTAAGGGCGA GCCGGGCTCT CGTGGAGGCG ATGGGGACAA AGGAGAGCGC 
GGTGACGATG GCCCTACAGG ACCAGATGGA CCTCGCGGCG AGAGAGGAAC TATTGGAGAG 
AAAGGGGTTC AAGGTTCCCC CGGAAACAGA GGTCCAAGAG GAGATCCCGG GGACCCTGGA 
CCCCGTGGAG AACCGGGGAG GGAGGGCACA CCTGGTCCCA ACGGAGACCC TGGTGTGAAA 
GGCAGAGAAG GGCCTCCTGG CTACAGAGGA GATGAAGGAC CATCTGGACC AGAAGGACCC 
AAAGGAATCA GAGGAATCAA AGGCGCTCCT GGAGACAGAG GCCTGATTGG CGACAGGGGA 
GAAGATGGTG CCCCAGGAAA TGGTACCGCA GGTTGTCCTG GCTTCCAGGG TTATCCTGGG 
ACCCGTGGAG ATCCTGGTGA GCCTGGTGGC AAGGGAACCC CTGGACCCAA AGGAGATGAC 
GGAGATCCCG GAGATCCAGG CCCCGATAAC GACATCCCAG GAACTCCAGG ACGTAAAGGA 
GCCAAAGGTC ACAGAGGACC CGACGGCAAT CCAGGACCTC CTGGGCCTCC TGGACCAGCA 
GGAACTGATG AATGTGAAAT TCTGGATATC ATCATGAAGC TCTGTTCTTG TTGTGAGTGT 
AAGTGTGGAT CTCTGGACCT GGCGTTCATT GTGGACAGCT CAGAGAGTAT TGGTGAATCA 
AACTTTGCTC TTGCCAAAGA CTTTATCATC ACAGTCATTG ACAGACTGAT CAAAGACCAG 
CAAGTCAAGT TTGCAGGTAA TGAGTCCAGA GTGAGTGTAG TGCAGTACAG TGGAGCAAGG 
GCCCAAGAAG CTGTCCAATT GGGATCCAAC GCTTTTAGCC TCACCGACTT CAAACAGAAA 
GTGAGAGACT TGAGATGGCT GGCTGAGGCC ACATACACAG GCGAGGCCCT TGATTTCGCT 
CTAAAAAACA CAATCAGTCT GATGCGGCAA GACAACCGAG TTGTTCTTGT CCTCACCGAC 
GGACGTTCTG ATACTGATAG GGACACGATT CCCCTCAACA TCCTCTGTGG TCAAAATGTC 
CGGGTGGGTG GTTTAGGAGT GAAGGACTAC GCAGGTCGTC AGCCCAACCA GGAGCGGCTT 
GATGAAATGA GTTGTAAGAA CGATCCCAAA CCAGGCTTTT CCTTTGTTCT GGACAACTTT 
GCCGAGCTGC TGGATGACAC TTTCCTGCAA AACCTCACAA CCAAAATCTG TCAAGATAAG 
AAGTGCCCGT CGGCACGACA AAGATGTCCC ATTTCTTTCG ATGAAAGTGC CGATATTTTG 
GTGATGATGG ACAGCTCGGC GAGTGTGGGC CAGAAGAACT TCGAGACAAG CAAGACCTTT 
GTCCGACGTT TGGCTGAGCG TTTCCTGACC GCAGAGAAAA AGAGGGTCGC CACTGTCAAA 
GTGGCCGTTG GCCAATACAG CCGGAGCAGC CGCATGGAGC AGCAGCTAAC TACCAATTTG 
ACCCTGCTGT CTCGTCGCAT TGAAGAGGCA GCCTTCCAAA ATGACGGCAC TAATTTATTA 
GAGGCCATGG AGTTTGCTAT TAAGAATTTA CGTGGCCGTG GAGACGCATC GGGAGGTGTA 
AAGAAGCTGG TGCTGTTCTC TGACGGCAGG TCTCAGGATA TCACTGATGC TGGGCTGGAG 
AAGCGCGTAC GTGAAGTGGC GGAAGCCGGG GTGGAGCTCT TTGTCATCGC CATTGGCACC 
CAGGTCAATG AGGCCAACTT GCGCACTCTT GTGAGCAAAG GGCGTCGGGA AGACATCATC 
TCTGCCCAGC GCCACCTGTT CCGCATCGGA GACTACCCCT CTCTGCTCAG AGGGGTCTTC 
TACCAAACCG TTTCCCGTAG GGTGTCCAAG TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010748123.2
CDS	225..3260
Translation	MGTFRGLLALLCLFLTEAQTQDRKNRFADCPVDLFFVLDTSESVALRQQHPNYYIDQIKAFTNQFIDELQNMRHVCERILTWNSGALHYSDEVEVVKELSDLSTEREALKKSVRDIQYIGKGTYTDCAIKRGLAELLIGGSSYQENKYIVVVTDGHPITGYKEPCGGVQEAANEARQHGVKVFAVAISPDQEDTRLSLIATDHTYRQNFTAVDDTRATKIGTIRTIINMIKNETRDTCCSFECTAPGGPKGLIGDPGSKGESGRPGMPGEKGDPGPKGSIGDPGPVGYSGMKGDRGGRGEKGERGHKGFKGDKGQRGRDGIDGRKGEAGFAGLPGCKGSPGSDGLQGDPGPKGDPGPYGIKGEKGDPGKDGLSGRPGNYGASGPKGEPGSRGGDGDKGERGDDGPTGPDGPRGERGTIGEKGVQGSPGNRGPRGDPGDPGPRGEPGREGTPGPNGDPGVKGREGPPGYRGDEGPSGPEGPKGIRGIKGAPGDRGLIGDRGEDGAPGNGTAGCPGFQGYPGTRGDPGEPGGKGTPGPKGDDGDPGDPGPDNDIPGTPGRKGAKGHRGPDGNPGPPGPPGPAGTDECEILDIIMKLCSCCECKCGSLDLAFIVDSSESIGESNFALAKDFIITVIDRLIKDQQVKFAGNESRVSVVQYSGARAQEAVQLGSNAFSLTDFKQKVRDLRWLAEATYTGEALDFALKNTISLMRQDNRVVLVLTDGRSDTDRDTIPLNILCGQNVRVGGLGVKDYAGRQPNQERLDEMSCKNDPKPGFSFVLDNFAELLDDTFLQNLTTKICQDKKCPSARQRCPISFDESADILVMMDSSASVGQKNFETSKTFVRRLAERFLTAEKKRVATVKVAVGQYSRSSRMEQQLTTNLTLLSRRIEEAAFQNDGTNLLEAMEFAIKNLRGRGDASGGVKKLVLFSDGRSQDITDAGLEKRVREVAEAGVELFVIAIGTQVNEANLRTLVSKGRREDIISAQRHLFRIGDYPSLLRGVFYQTVSRRVSKS

Target ORF information:

RefSeq Version	XM_010749821.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea collagen type VI alpha 1 chain (col6a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010749821.2

ORF Insert Sequence:

ATGGGGACTT TCAGAGGTCT CCTTGCGCTG CTGTGCCTTT TCCTGACTGA GGCGCAAACG 
CAGGACCGGA AAAACAGATT TGCGGATTGT CCAGTTGACC TCTTCTTTGT TCTGGATACA 
TCTGAGAGTG TCGCCCTACG ACAGCAACAT CCCAACTATT ATATTGACCA GATCAAAGCA 
TTCACCAATC AATTCATAGA TGAACTCCAG AACATGCGTC ATGTATGTGA GCGCATCCTG 
ACATGGAACT CAGGAGCACT CCACTACAGT GACGAAGTCG AAGTTGTAAA AGAGCTGAGT 
GACCTGTCGA CTGAGCGCGA GGCCCTGAAG AAAAGCGTCA GAGACATTCA ATACATCGGC 
AAGGGCACAT ATACTGACTG TGCCATCAAG AGAGGTCTTG CTGAGCTGCT TATTGGGGGC 
TCCAGCTACC AGGAGAACAA GTACATCGTG GTGGTGACTG ATGGTCACCC TATCACTGGT 
TACAAAGAGC CATGTGGAGG TGTGCAGGAG GCTGCTAATG AGGCCAGGCA ACATGGAGTC 
AAAGTGTTTG CTGTCGCCAT TTCACCTGAC CAGGAGGACA CCAGGCTGTC ACTCATTGCC 
ACAGACCACA CCTACAGACA GAACTTCACT GCTGTAGATG ACACCAGAGC CACCAAGATC 
GGAACCATTA GAACCATCAT CAATATGATT AAAAACGAGA CAAGGGATAC GTGTTGCTCC 
TTCGAATGCA CTGCTCCAGG AGGGCCAAAG GGATTGATAG GAGACCCTGG ATCCAAGGGA 
GAGTCAGGTA GACCAGGAAT GCCAGGAGAG AAAGGAGATC CTGGTCCCAA GGGCAGCATT 
GGAGATCCTG GTCCAGTTGG TTACAGTGGA ATGAAGGGAG ACCGTGGTGG CAGAGGAGAA 
AAGGGTGAAA GAGGACACAA AGGCTTCAAG GGAGACAAAG GTCAAAGGGG AAGGGATGGA 
ATTGATGGAC GCAAGGGTGA GGCTGGTTTC GCTGGTCTTC CCGGCTGTAA AGGATCTCCC 
GGATCAGATG GCCTACAGGG AGATCCTGGA CCAAAGGGAG ACCCTGGTCC TTATGGAATA 
AAAGGAGAAA AAGGAGATCC TGGAAAAGAC GGCCTGTCTG GAAGACCTGG AAACTATGGT 
GCATCGGGAC CTAAGGGCGA GCCGGGCTCT CGTGGAGGCG ATGGGGACAA AGGAGAGCGC 
GGTGACGATG GCCCTACAGG ACCAGATGGA CCTCGCGGCG AGAGAGGAAC TATTGGAGAG 
AAAGGGGTTC AAGGTTCCCC CGGAAACAGA GGTCCAAGAG GAGATCCCGG GGACCCTGGA 
CCCCGTGGAG AACCGGGGAG GGAGGGCACA CCTGGTCCCA ACGGAGACCC TGGTGTGAAA 
GGCAGAGAAG GGCCTCCTGG CTACAGAGGA GATGAAGGAC CATCTGGACC AGAAGGACCC 
AAAGGAATCA GAGGAATCAA AGGCGCTCCT GGAGACAGAG GCCTGATTGG CGACAGGGGA 
GAAGATGGTG CCCCAGGAAA TGGTACCGCA GGTTGTCCTG GCTTCCAGGG TTATCCTGGG 
ACCCGTGGAG ATCCTGGTGA GCCTGGTGGC AAGGGAACCC CTGGACCCAA AGGAGATGAC 
GGAGATCCCG GAGATCCAGG CCCCGATAAC GACATCCCAG GAACTCCAGG ACGTAAAGGA 
GCCAAAGGTC ACAGAGGACC CGACGGCAAT CCAGGACCTC CTGGGCCTCC TGGACCAGCA 
GGAACTGATG AATGTGAAAT TCTGGATATC ATCATGAAGC TCTGTTCTTG TTGTGAGTGT 
AAGTGTGGAT CTCTGGACCT GGCGTTCATT GTGGACAGCT CAGAGAGTAT TGGTGAATCA 
AACTTTGCTC TTGCCAAAGA CTTTATCATC ACAGTCATTG ACAGACTGAT CAAAGACCAG 
CAAGTCAAGT TTGCAGGTAA TGAGTCCAGA GTGAGTGTAG TGCAGTACAG TGGAGCAAGG 
GCCCAAGAAG CTGTCCAATT GGGATCCAAC GCTTTTAGCC TCACCGACTT CAAACAGAAA 
GTGAGAGACT TGAGATGGCT GGCTGAGGCC ACATACACAG GCGAGGCCCT TGATTTCGCT 
CTAAAAAACA CAATCAGTCT GATGCGGCAA GACAACCGAG TTGTTCTTGT CCTCACCGAC 
GGACGTTCTG ATACTGATAG GGACACGATT CCCCTCAACA TCCTCTGTGG TCAAAATGTC 
CGGGTGGGTG GTTTAGGAGT GAAGGACTAC GCAGGTCGTC AGCCCAACCA GGAGCGGCTT 
GATGAAATGA GTTGTAAGAA CGATCCCAAA CCAGGCTTTT CCTTTGTTCT GGACAACTTT 
GCCGAGCTGC TGGATGACAC TTTCCTGCAA AACCTCACAA CCAAAATCTG TCAAGATAAG 
AAGTGCCCGT CGGCACGACA AAGATGTCCC ATTTCTTTCG ATGAAAGTGC CGATATTTTG 
GTGATGATGG ACAGCTCGGC GAGTGTGGGC CAGAAGAACT TCGAGACAAG CAAGACCTTT 
GTCCGACGTT TGGCTGAGCG TTTCCTGACC GCAGAGAAAA AGAGGGTCGC CACTGTCAAA 
GTGGCCGTTG GCCAATACAG CCGGAGCAGC CGCATGGAGC AGCAGCTAAC TACCAATTTG 
ACCCTGCTGT CTCGTCGCAT TGAAGAGGCA GCCTTCCAAA ATGACGGCAC TAATTTATTA 
GAGGCCATGG AGTTTGCTAT TAAGAATTTA CGTGGCCGTG GAGACGCATC GGGAGGTGTA 
AAGAAGCTGG TGCTGTTCTC TGACGGCAGG TCTCAGGATA TCACTGATGC TGGGCTGGAG 
AAGCGCGTAC GTGAAGTGGC GGAAGCCGGG GTGGAGCTCT TTGTCATCGC CATTGGCACC 
CAGGTCAATG AGGCCAACTT GCGCACTCTT GTGAGCAAAG GGCGTCGGGA AGACATCATC 
TCTGCCCAGC GCCACCTGTT CCGCATCGGA GACTACCCCT CTCTGCTCAG AGGGGTCTTC 
TACCAAACCG TTTCCCGTAG GGTGTCCAAG TCCTGA