LOC104928886 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104928886
Entrez Gene ID	104928886
Full Name	monofunctional C1-tetrahydrofolate synthase, mitochondrial-like
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019259006.2	XP_019114551.2	monofunctional C1-tetrahydrofolate synthase, mitochondrial
XM_019259006.1	XP_019114551.1	monofunctional C1-tetrahydrofolate synthase, mitochondrial-like

Pathways from BioSystems
Gene Ontology

The following LOC104928886 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104928886 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa200173	XM_019259006.2 Latest version!	Larimichthys crocea methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (mthfd1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OLa89516	XM_019259006.1 Latest version!	Larimichthys crocea monofunctional C1-tetrahydrofolate synthase, mitochondrial-like (LOC104928886), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104928886 ( XM_019259006.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019114551.2 Larimichthys crocea methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (mthfd1l), mRNA.

Price & Availability↑

CloneID	OLa200173
Clone ID Related Accession (Same CDS sequence)	XM_019259006.2
Accession Version	XM_019259006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019259006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGT CTACCATTCG CAATGTGTGT GGCAGCCTGC GGTCACCTCT AGTCCGTCAG 
GGAGGCGGTG TCAGCGGCTT GTCGCGGAGA TGCCCCCCGG TTAGTCCCAC CAACAGCCGG 
TTGATCATCC GAACGGCGAG CGCTACTACG AGCGGCGGAA CCAACCCGAG AGTAGCCGTA 
AAGTTTGGGA AACAGCCCCA AAAGGGTGAT GGGTTTGAAT TCAACACCTC TTTGAGGGAA 
GTTGTTCAGG GCACCAAGGA GGCAATGGTC GCTCTGCAGA GAAAGAATCC AGCAATCCAG 
CCTTTGTTAG TCATTATACA GGCAGGTGAA GATGATAACT TGCTGGAGAT CAATAAAAAA 
ATAGCAGGAA GGATCGGCTT AAACATCACT CAGATTTGTC TGGCAAAAGA GTGCAGTGAA 
GATGAAATCG TTGAGGAGGT GTTGAAGCTG AATGAAGACC CCAGGGTGCA TGGAGTCTAC 
CTCCACCTCC CTCCTGCTTG TCTCACCAGT CGAGTGCTGA ACACTCTCAA ACCTGAAAAG 
GATGTAGATG GGATATCTGA TTTAAATATG GGCCGTCTGG TACGTGGAGA CCTGAGTAAA 
GGCTTTGTGC CACCCTTGGC CAGCGCTGTC CTGGATCTGC TGGAGAAACA TGATGCTCCT 
TTGGAAGGGA AAACCGTGTT TCTCGCTGGA GGAGAAGGAC CCCTCGGCGT GGCTCTGCAG 
TGCCTGATTG AAAGAAATGG AATGGTTGCT TTTAAGAGTC ATTGGAGCTC CAACAGCCTA 
CAGAGACAGG TGATGCAAGC TGATGCTGTA GTCCTGTTAG GGCCAGGCAA CATGGATGTC 
CCACCCACCT GGGTTAGACC TGGAGCTGTT GTGATCCGCT GTGAGCCCAC CCTGGAGACA 
GATGAACATG CCATTGAGAT ATCCTCAAAG TCTGGGTTAG GATTCCTCAC AGCAGCCTAC 
AGAACACGGA ATGTGGTGCA TAGTTGCAGT CGTTGGATCC AGGAACAGCA GTACAGACCC 
TGGCATCTGC GCAGCCTCAA ACTGCAGCCC CTGACCCCTG TACCAAGTGA CATAGAGATT 
TCCAGAGCTC AGACGCCCAA GCCAGTGGAC CAGCTGGCTG AGGAGATTGG TCTGCTCCCA 
GAAGAGCTTG AAGCCTATGG CAGAAGCAAA GCTAAGGTCC AACTCTCCCT GTTAGACCGC 
CTCCACACAC AGCCTGATGG GAAATTAGTT CTGGTTGCTG GTATAACTCC CACTCCACTG 
GGCGAAGGTA AAAGCACGGT GACCATCGGT CTAGTCCAGG CGCTGTCTGC CCACCTGAAG 
CTAAACTCCT TCGCTTGTCT GCGACAGCCT TCGCAGGGAC CCACTTTTGG GGTTAAAGGG 
GGAGCTGCAG GAGGGGGATA TGCCCAGGTC ATCCCTATGG AGGAGTTCAA CCTCCATCTG 
ACTGGTGACA TTCATGCCAT CACAGCAGCC AATAACCTGG TGGCGGCAGC TATCGACGCC 
AGGATGCTCC ACGAGGCAAC GCAATCAGAC AAGGCCCTGT TCAATAGACT GGTCCCTTCA 
GTGAATGGAG TCAGAAAATT TTCACCCATC CAAATCTCCA GGCTACGACG TCTAGGGATC 
AATAAAATAG ACCCAGCATC TCTTACACCC CAGGAGGTCA GCACCTTTGT CCGTCTTGAC 
CTTGACCCTT CCAAGATCAC CTGGCAGAGA GTTCTTGACA CAAACGATCG TTTCTTGAGG 
AAGATCACAG TTGGACAGGC GAGCACTGAA AAAGGACAGA TCAGAGAGAC TGGGTTCGAC 
ATCGCAGTGG CCAGCGAGAT TATGGCCATC CTGGCTCTGG CAGACAGCCT GGAGGACATG 
AAAAACCGAC TGGCACGCAT GGTGGTGGGG ACCAGCCGCT CTGGACAGCC TGTCACCGCA 
GAGGACCTGG GTGTGAGTGG AGCACTGGCA GTGTTGATGA AAGATGCCAT CAAGCCCACA 
TTGATGCAGA CCCTCGAGGG TACGCCAGTG TTTGTCCATG CTGGGCCCTT TGCCAACATC 
GCCCATGGCA ACTCCTCGGT GCTGGCAGAC AAGTTGGCTT TGAAGTTGGT CGGACGGGAC 
GGCTTTGTGG TGACAGAAGC TGGTTTTGGA GCAGACATCG GCATGGAGAA GTTCTTCAAC 
ATCAAATGCC GTGCTTCAGG GCTGAGGCCG AATGTGGTGG TGCTGGTCGC AACTGTCCGA 
GCCTTAAAGA TGCATGGTGG TGGCCCAAAT GTGTCAGCAG GAGCACCTCT TCCCAGAGAG 
TACATTGATG AGAATCTGAG TTTAGTTGCA GGTGGTTGTC ATAGCAATCT CAGGAAGCAG 
ATCCAAATCG CTGACGTATT CGGGGTACCA GTCGTGGTTG CGCTCAATGT CTTCAAGACA 
GACACACAGG CAGAAATTGA CCTTGTGTGT CAGCTTGCAA AAGAGTGTGG AGCCTCTGAC 
GCCGTGCCAT GTCACCACTG GGCGCAGGGC GGGCGAGGTT CCATAGAGCT GGCCCAAGCT 
GTGAATGAGG CTGCCAGCAG ACCCAGCAAC TTCCAGTTCC TGTACAACAA AGAGATGCCG 
ATAGTGGAGA AGATCAGAAC AATCGCCCAA AGAGTGTATG GAGCTGATGA CATTGAGCTG 
TCTCCAGAGG CCGGGGCCAA GATAGATTAC TACAATCAAC AGGGCTACGG AACATTGCCC 
ATCTGCATGG CCAAGACTCA CCTGTCCCTG TCCCACATGC CAGACAAGAA GGGTGCACCC 
ACTGGGTTTG TTTTGCCAAT CAGAGATGTC CGTGCTAGCA TTGGCGCTGG CTTCATCTAC 
CCACTTGTGG GAACGATGAG CACCATGCCT GGTCTGCCAA CCCGACCCTG TTTCTATGAC 
ATTGACCTTG ATCCGGTCAC GGAGGAGATC ACTGGGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019114551.2
CDS	137..3061
Translation	MKLSTIRNVCGSLRSPLVRQGGGVSGLSRRCPPVSPTNSRLIIRTASATTSGGTNPRVAVKFGKQPQKGDGFEFNTSLREVVQGTKEAMVALQRKNPAIQPLLVIIQAGEDDNLLEINKKIAGRIGLNITQICLAKECSEDEIVEEVLKLNEDPRVHGVYLHLPPACLTSRVLNTLKPEKDVDGISDLNMGRLVRGDLSKGFVPPLASAVLDLLEKHDAPLEGKTVFLAGGEGPLGVALQCLIERNGMVAFKSHWSSNSLQRQVMQADAVVLLGPGNMDVPPTWVRPGAVVIRCEPTLETDEHAIEISSKSGLGFLTAAYRTRNVVHSCSRWIQEQQYRPWHLRSLKLQPLTPVPSDIEISRAQTPKPVDQLAEEIGLLPEELEAYGRSKAKVQLSLLDRLHTQPDGKLVLVAGITPTPLGEGKSTVTIGLVQALSAHLKLNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMLHEATQSDKALFNRLVPSVNGVRKFSPIQISRLRRLGINKIDPASLTPQEVSTFVRLDLDPSKITWQRVLDTNDRFLRKITVGQASTEKGQIRETGFDIAVASEIMAILALADSLEDMKNRLARMVVGTSRSGQPVTAEDLGVSGALAVLMKDAIKPTLMQTLEGTPVFVHAGPFANIAHGNSSVLADKLALKLVGRDGFVVTEAGFGADIGMEKFFNIKCRASGLRPNVVVLVATVRALKMHGGGPNVSAGAPLPREYIDENLSLVAGGCHSNLRKQIQIADVFGVPVVVALNVFKTDTQAEIDLVCQLAKECGASDAVPCHHWAQGGRGSIELAQAVNEAASRPSNFQFLYNKEMPIVEKIRTIAQRVYGADDIELSPEAGAKIDYYNQQGYGTLPICMAKTHLSLSHMPDKKGAPTGFVLPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDPVTEEITGLF

Target ORF information:

RefSeq Version	XM_019259006.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like (mthfd1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019259006.2

ORF Insert Sequence:

ATGAAGTTGT CTACCATTCG CAATGTGTGT GGCAGCCTGC GGTCACCTCT AGTCCGTCAG 
GGAGGCGGTG TCAGCGGCTT GTCGCGGAGA TGCCCCCCGG TTAGTCCCAC CAACAGCCGG 
TTGATCATCC GAACGGCGAG CGCTACTACG AGCGGCGGAA CCAACCCGAG AGTAGCCGTA 
AAGTTTGGGA AACAGCCCCA AAAGGGTGAT GGGTTTGAAT TCAACACCTC TTTGAGGGAA 
GTTGTTCAGG GCACCAAGGA GGCAATGGTC GCTCTGCAGA GAAAGAATCC AGCAATCCAG 
CCTTTGTTAG TCATTATACA GGCAGGTGAA GATGATAACT TGCTGGAGAT CAATAAAAAA 
ATAGCAGGAA GGATCGGCTT AAACATCACT CAGATTTGTC TGGCAAAAGA GTGCAGTGAA 
GATGAAATCG TTGAGGAGGT GTTGAAGCTG AATGAAGACC CCAGGGTGCA TGGAGTCTAC 
CTCCACCTCC CTCCTGCTTG TCTCACCAGT CGAGTGCTGA ACACTCTCAA ACCTGAAAAG 
GATGTAGATG GGATATCTGA TTTAAATATG GGCCGTCTGG TACGTGGAGA CCTGAGTAAA 
GGCTTTGTGC CACCCTTGGC CAGCGCTGTC CTGGATCTGC TGGAGAAACA TGATGCTCCT 
TTGGAAGGGA AAACCGTGTT TCTCGCTGGA GGAGAAGGAC CCCTCGGCGT GGCTCTGCAG 
TGCCTGATTG AAAGAAATGG AATGGTTGCT TTTAAGAGTC ATTGGAGCTC CAACAGCCTA 
CAGAGACAGG TGATGCAAGC TGATGCTGTA GTCCTGTTAG GGCCAGGCAA CATGGATGTC 
CCACCCACCT GGGTTAGACC TGGAGCTGTT GTGATCCGCT GTGAGCCCAC CCTGGAGACA 
GATGAACATG CCATTGAGAT ATCCTCAAAG TCTGGGTTAG GATTCCTCAC AGCAGCCTAC 
AGAACACGGA ATGTGGTGCA TAGTTGCAGT CGTTGGATCC AGGAACAGCA GTACAGACCC 
TGGCATCTGC GCAGCCTCAA ACTGCAGCCC CTGACCCCTG TACCAAGTGA CATAGAGATT 
TCCAGAGCTC AGACGCCCAA GCCAGTGGAC CAGCTGGCTG AGGAGATTGG TCTGCTCCCA 
GAAGAGCTTG AAGCCTATGG CAGAAGCAAA GCTAAGGTCC AACTCTCCCT GTTAGACCGC 
CTCCACACAC AGCCTGATGG GAAATTAGTT CTGGTTGCTG GTATAACTCC CACTCCACTG 
GGCGAAGGTA AAAGCACGGT GACCATCGGT CTAGTCCAGG CGCTGTCTGC CCACCTGAAG 
CTAAACTCCT TCGCTTGTCT GCGACAGCCT TCGCAGGGAC CCACTTTTGG GGTTAAAGGG 
GGAGCTGCAG GAGGGGGATA TGCCCAGGTC ATCCCTATGG AGGAGTTCAA CCTCCATCTG 
ACTGGTGACA TTCATGCCAT CACAGCAGCC AATAACCTGG TGGCGGCAGC TATCGACGCC 
AGGATGCTCC ACGAGGCAAC GCAATCAGAC AAGGCCCTGT TCAATAGACT GGTCCCTTCA 
GTGAATGGAG TCAGAAAATT TTCACCCATC CAAATCTCCA GGCTACGACG TCTAGGGATC 
AATAAAATAG ACCCAGCATC TCTTACACCC CAGGAGGTCA GCACCTTTGT CCGTCTTGAC 
CTTGACCCTT CCAAGATCAC CTGGCAGAGA GTTCTTGACA CAAACGATCG TTTCTTGAGG 
AAGATCACAG TTGGACAGGC GAGCACTGAA AAAGGACAGA TCAGAGAGAC TGGGTTCGAC 
ATCGCAGTGG CCAGCGAGAT TATGGCCATC CTGGCTCTGG CAGACAGCCT GGAGGACATG 
AAAAACCGAC TGGCACGCAT GGTGGTGGGG ACCAGCCGCT CTGGACAGCC TGTCACCGCA 
GAGGACCTGG GTGTGAGTGG AGCACTGGCA GTGTTGATGA AAGATGCCAT CAAGCCCACA 
TTGATGCAGA CCCTCGAGGG TACGCCAGTG TTTGTCCATG CTGGGCCCTT TGCCAACATC 
GCCCATGGCA ACTCCTCGGT GCTGGCAGAC AAGTTGGCTT TGAAGTTGGT CGGACGGGAC 
GGCTTTGTGG TGACAGAAGC TGGTTTTGGA GCAGACATCG GCATGGAGAA GTTCTTCAAC 
ATCAAATGCC GTGCTTCAGG GCTGAGGCCG AATGTGGTGG TGCTGGTCGC AACTGTCCGA 
GCCTTAAAGA TGCATGGTGG TGGCCCAAAT GTGTCAGCAG GAGCACCTCT TCCCAGAGAG 
TACATTGATG AGAATCTGAG TTTAGTTGCA GGTGGTTGTC ATAGCAATCT CAGGAAGCAG 
ATCCAAATCG CTGACGTATT CGGGGTACCA GTCGTGGTTG CGCTCAATGT CTTCAAGACA 
GACACACAGG CAGAAATTGA CCTTGTGTGT CAGCTTGCAA AAGAGTGTGG AGCCTCTGAC 
GCCGTGCCAT GTCACCACTG GGCGCAGGGC GGGCGAGGTT CCATAGAGCT GGCCCAAGCT 
GTGAATGAGG CTGCCAGCAG ACCCAGCAAC TTCCAGTTCC TGTACAACAA AGAGATGCCG 
ATAGTGGAGA AGATCAGAAC AATCGCCCAA AGAGTGTATG GAGCTGATGA CATTGAGCTG 
TCTCCAGAGG CCGGGGCCAA GATAGATTAC TACAATCAAC AGGGCTACGG AACATTGCCC 
ATCTGCATGG CCAAGACTCA CCTGTCCCTG TCCCACATGC CAGACAAGAA GGGTGCACCC 
ACTGGGTTTG TTTTGCCAAT CAGAGATGTC CGTGCTAGCA TTGGCGCTGG CTTCATCTAC 
CCACTTGTGG GAACGATGAG CACCATGCCT GGTCTGCCAA CCCGACCCTG TTTCTATGAC 
ATTGACCTTG ATCCGGTCAC GGAGGAGATC ACTGGGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104928886 ( XM_019259006.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019114551.1 Larimichthys crocea monofunctional C1-tetrahydrofolate synthase, mitochondrial-like (LOC104928886), mRNA.

Price & Availability↑

CloneID	OLa89516
Clone ID Related Accession (Same CDS sequence)	XM_019259006.1
Accession Version	XM_019259006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	monofunctional C1-tetrahydrofolate synthase, mitochondrial-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608227.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGT CTACCATTCG CAATGTGTGT GGCAGCCTGC GGTCACCTCT AGTCCGTCAG 
GGAGGCGGTG TCAGCGGCTT GTCGCGGAGA TGCCCCCCGG TTAGTCCCAC CAACAGCCGG 
TTGATCATCC GAACGGCGAG CGCTACTACG AGCGGCGGCA CCAACCCGAG AGTAGCCGTA 
AAGTTTGGGA AACAGCCCCA AAAGGGTGAT GGGTTTGAAT TCAACACCTC TTTGAGGGAA 
GTTGTTCAGG GCACCAAGGA GGCAATGGTC GCTCTGCAGA GAAAGAATCC AGCAATCCAG 
CCTTTGTTAG TCATTATACA GGCAGGTGAA GATGATAACT TGCTGGAGAT CAATAAAAAA 
ATAGCAGGAA GGATCGGCTT AAACATCACT CAGATTTGTC TGGCAAAAGA GTGCAGTGAA 
GATGAAATCG TTGAGGAGGT GTTGAAGCTG AATGAAGACC CCAGGGTGCA TGGAGTCTAC 
CTCCACCTCC CTCCTGCTTG TCTCACCAGT CGAGTGCTGA ACACTCTCAA ACCTGAAAAG 
GATGTAGATG GGATATCTGA TTTAAATATG GGCCGTCTGG TACGTGGAGA CCTGAGTAAA 
GGCTTTGTGC CACCCTTGGC CAGCGCTGTC CTGGATCTGC TGGAGAAACA TGATGCTCCT 
TTGGAAGGGA AAACCGTGTT TCTCGCTGGA GGAGAAGGAC CCCTCGGCGT GGCTCTGCAG 
TGCCTGATTG AAAGAAATGG AATGGTTGCT TTTAAGAGTC ATTGGAGCTC CAACAGCCTA 
CAGAGACAGG TGATGCAAGC TGATGCTGTA GTCCTGTTAG GCCCTGGGAA CATGGATGTC 
CCACCCACAT GGGTTAGACC TGGAGCTGTT GTGATCCGCT GTGAGCCCAC CCTGGAGACA 
GATGAACATG CCATTGAGAT ATCCTCAAAG TCTGGGTTAG GATTCCTCAC AGCAGCCTAC 
AGAACACGGA ATGTGGTGCA TAGTTGCAGT CGTTGGATCC AGGAACAGCA GTACAGACCC 
TGGCATCTGC GCAGCCTCAA ACTGCAGCCC CTGACCCCTG TACCAAGTGA CATAGAGATT 
TCCAGAGCTC AGACGCCCAA GCCAGTGGAC CAGCTGGCTG AGGAGATTGG TCTGCTCCCA 
GAAGAGCTTG AAGCCTATGG CAGAAGCAAA GCTAAGGTCC AACTCTCCCT GTTAGACCGC 
CTCCACAAAC AGCCTGATGG GAAATTAGTT CTGGTTGCTG GTATAACTCC CACTCCACTG 
GGCGAAGGTA AAAGCACGGT GACCATCGGT CTAGTCCAGG CGCTGTCTGC CCACCTGAAG 
CTAAACTCCT TCGCTTGTCT GCGACAGCCT TCGCAGGGAC CCACTTTTGG GGTTAAAGGG 
GGAGCTGCAG GAGGGGGATA TGCCCAGGTC ATCCCTATGG AGGAGTTCAA CCTCCATCTG 
ACTGGTGACA TTCATGCCAT CACAGCAGCC AATAACCTGG TGGCGGCAGC TATCGACGCT 
AGGATGCTCC ACGAGGCAAC GCAATCAGAC AAGGCCCTGT TCAATAGACT GGTCCCTTCA 
GTGAATGGAG TCAGAAAATT TTCACCCATC CAAATCTCCA GGCTACGACG TCTAGGGATC 
AATAAAATAG ACCCAGCATC TCTTACACCC CAGGAGGTCA GCACCTTTGT CCGTCTTGAC 
CTTGACCCTT CCAAGATCAC CTGGCAGAGA GTTCTTGACA CAAATGATCG TTTCTTGAGA 
AAGATCACAG TTGGACAGGC AAGCACTGAA AAAGGACAGA TCAGAGAGAC TGGGTTCGAC 
ATCGCAGTGG CCAGCGAGAT TATGGCCATC CTGGCTCTGG CAGACAGCCT GGAGGACATG 
AAAATCCGAC TGGCACGCAT GGTGGTGGGG ACCAGCCGCT CTGGACAGCC TGTCACCGCA 
GAGGACCTGG GTGTGAGTGG AGCACTGGCA GTGTTGATGA AAGATGCCAT CAAGCCCACG 
TTGATGCAGA CCCTCGAGGG TACGCCAGTG TTTGTCCATG CTGGGCCCTT TGCCAACATC 
GCCCATGGCA ACTCCTCGGT GCTGGCAGAC AAGCTGGCTT TGAAGTTGGT CGGACGGGAC 
GGCTTTGTGG TGACAGAAGC TGGTTTTGGA GCAGACATCG GCATGGAGAA GTTCTTCAAC 
ATCAAATGCC GTGCTTCAGG GCTGAGGCCG AATGTGGTGG TGCTGGTCGC AACTGTCCGA 
GCCTTAAAGA TGCATGGTGG TGGCCCAAAT GTGTCAGCAG GAGCACCTCT TCCCAGAGAG 
TACATTGATG AGAATCTGAG TTTAGTTGCA GGTGGTTGTC ATAGCAATCT CAGGAAGCAG 
ATCCAAATCG CTGACGTATT CGGGGTACCA GTCGTGGTTG CGCTCAATGT CTTCAAGACA 
GACACACAGG CAGAAATTGA CCTTGTGTGT CAGCTTGCAA AAGAGTGTGG AGCCTCTGAC 
GCCGTGCCAT GTCACCACTG GGCGCAGGGC GGGCGAGGTT CCATAGAGCT GGCCCAAGCT 
GTGAATGAGG CTGCCAGCAG ACCCAGCAAC TTCCAGTTCC TGTACAACAA AGAGATGCCG 
ATAGTGGAGA AGATCAGAAC AATCGCCCAA AGAGTGTATG GAGCTGATGA CATTGAGCTG 
TCTCCAGAGG CCGGGGCCAA GATAGATTAC TACAATCAAC AGGGCTACGG AACATTGCCC 
ATCTGCATGG CCAAGACTCA CCTGTCCCTG TCCCACATGC CAGACAAGAA GGGTGCACCC 
ACTGGGTTTG TTTTGCCAAT CAGAGATGTC CGTGCTAGCA TTGGCGCTGG CTTCATCTAC 
CCACTTGTGG GAACGATGAG CACCATGCCT GGTCTGCCAA CCCGACCCTG TTTCTATGAC 
ATTGACCTTG ATCCGGTCAC GGAGGAGATC ACTGGGCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019114551.1
CDS	503..3427
Translation	MKLSTIRNVCGSLRSPLVRQGGGVSGLSRRCPPVSPTNSRLIIRTASATTSGGTNPRVAVKFGKQPQKGDGFEFNTSLREVVQGTKEAMVALQRKNPAIQPLLVIIQAGEDDNLLEINKKIAGRIGLNITQICLAKECSEDEIVEEVLKLNEDPRVHGVYLHLPPACLTSRVLNTLKPEKDVDGISDLNMGRLVRGDLSKGFVPPLASAVLDLLEKHDAPLEGKTVFLAGGEGPLGVALQCLIERNGMVAFKSHWSSNSLQRQVMQADAVVLLGPGNMDVPPTWVRPGAVVIRCEPTLETDEHAIEISSKSGLGFLTAAYRTRNVVHSCSRWIQEQQYRPWHLRSLKLQPLTPVPSDIEISRAQTPKPVDQLAEEIGLLPEELEAYGRSKAKVQLSLLDRLHKQPDGKLVLVAGITPTPLGEGKSTVTIGLVQALSAHLKLNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARMLHEATQSDKALFNRLVPSVNGVRKFSPIQISRLRRLGINKIDPASLTPQEVSTFVRLDLDPSKITWQRVLDTNDRFLRKITVGQASTEKGQIRETGFDIAVASEIMAILALADSLEDMKIRLARMVVGTSRSGQPVTAEDLGVSGALAVLMKDAIKPTLMQTLEGTPVFVHAGPFANIAHGNSSVLADKLALKLVGRDGFVVTEAGFGADIGMEKFFNIKCRASGLRPNVVVLVATVRALKMHGGGPNVSAGAPLPREYIDENLSLVAGGCHSNLRKQIQIADVFGVPVVVALNVFKTDTQAEIDLVCQLAKECGASDAVPCHHWAQGGRGSIELAQAVNEAASRPSNFQFLYNKEMPIVEKIRTIAQRVYGADDIELSPEAGAKIDYYNQQGYGTLPICMAKTHLSLSHMPDKKGAPTGFVLPIRDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDPVTEEITGLF

Target ORF information:

RefSeq Version	XM_019259006.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea monofunctional C1-tetrahydrofolate synthase, mitochondrial-like (LOC104928886), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019259006.1

ORF Insert Sequence:

ATGAAGTTGT CTACCATTCG CAATGTGTGT GGCAGCCTGC GGTCACCTCT AGTCCGTCAG 
GGAGGCGGTG TCAGCGGCTT GTCGCGGAGA TGCCCCCCGG TTAGTCCCAC CAACAGCCGG 
TTGATCATCC GAACGGCGAG CGCTACTACG AGCGGCGGCA CCAACCCGAG AGTAGCCGTA 
AAGTTTGGGA AACAGCCCCA AAAGGGTGAT GGGTTTGAAT TCAACACCTC TTTGAGGGAA 
GTTGTTCAGG GCACCAAGGA GGCAATGGTC GCTCTGCAGA GAAAGAATCC AGCAATCCAG 
CCTTTGTTAG TCATTATACA GGCAGGTGAA GATGATAACT TGCTGGAGAT CAATAAAAAA 
ATAGCAGGAA GGATCGGCTT AAACATCACT CAGATTTGTC TGGCAAAAGA GTGCAGTGAA 
GATGAAATCG TTGAGGAGGT GTTGAAGCTG AATGAAGACC CCAGGGTGCA TGGAGTCTAC 
CTCCACCTCC CTCCTGCTTG TCTCACCAGT CGAGTGCTGA ACACTCTCAA ACCTGAAAAG 
GATGTAGATG GGATATCTGA TTTAAATATG GGCCGTCTGG TACGTGGAGA CCTGAGTAAA 
GGCTTTGTGC CACCCTTGGC CAGCGCTGTC CTGGATCTGC TGGAGAAACA TGATGCTCCT 
TTGGAAGGGA AAACCGTGTT TCTCGCTGGA GGAGAAGGAC CCCTCGGCGT GGCTCTGCAG 
TGCCTGATTG AAAGAAATGG AATGGTTGCT TTTAAGAGTC ATTGGAGCTC CAACAGCCTA 
CAGAGACAGG TGATGCAAGC TGATGCTGTA GTCCTGTTAG GCCCTGGGAA CATGGATGTC 
CCACCCACAT GGGTTAGACC TGGAGCTGTT GTGATCCGCT GTGAGCCCAC CCTGGAGACA 
GATGAACATG CCATTGAGAT ATCCTCAAAG TCTGGGTTAG GATTCCTCAC AGCAGCCTAC 
AGAACACGGA ATGTGGTGCA TAGTTGCAGT CGTTGGATCC AGGAACAGCA GTACAGACCC 
TGGCATCTGC GCAGCCTCAA ACTGCAGCCC CTGACCCCTG TACCAAGTGA CATAGAGATT 
TCCAGAGCTC AGACGCCCAA GCCAGTGGAC CAGCTGGCTG AGGAGATTGG TCTGCTCCCA 
GAAGAGCTTG AAGCCTATGG CAGAAGCAAA GCTAAGGTCC AACTCTCCCT GTTAGACCGC 
CTCCACAAAC AGCCTGATGG GAAATTAGTT CTGGTTGCTG GTATAACTCC CACTCCACTG 
GGCGAAGGTA AAAGCACGGT GACCATCGGT CTAGTCCAGG CGCTGTCTGC CCACCTGAAG 
CTAAACTCCT TCGCTTGTCT GCGACAGCCT TCGCAGGGAC CCACTTTTGG GGTTAAAGGG 
GGAGCTGCAG GAGGGGGATA TGCCCAGGTC ATCCCTATGG AGGAGTTCAA CCTCCATCTG 
ACTGGTGACA TTCATGCCAT CACAGCAGCC AATAACCTGG TGGCGGCAGC TATCGACGCT 
AGGATGCTCC ACGAGGCAAC GCAATCAGAC AAGGCCCTGT TCAATAGACT GGTCCCTTCA 
GTGAATGGAG TCAGAAAATT TTCACCCATC CAAATCTCCA GGCTACGACG TCTAGGGATC 
AATAAAATAG ACCCAGCATC TCTTACACCC CAGGAGGTCA GCACCTTTGT CCGTCTTGAC 
CTTGACCCTT CCAAGATCAC CTGGCAGAGA GTTCTTGACA CAAATGATCG TTTCTTGAGA 
AAGATCACAG TTGGACAGGC AAGCACTGAA AAAGGACAGA TCAGAGAGAC TGGGTTCGAC 
ATCGCAGTGG CCAGCGAGAT TATGGCCATC CTGGCTCTGG CAGACAGCCT GGAGGACATG 
AAAATCCGAC TGGCACGCAT GGTGGTGGGG ACCAGCCGCT CTGGACAGCC TGTCACCGCA 
GAGGACCTGG GTGTGAGTGG AGCACTGGCA GTGTTGATGA AAGATGCCAT CAAGCCCACG 
TTGATGCAGA CCCTCGAGGG TACGCCAGTG TTTGTCCATG CTGGGCCCTT TGCCAACATC 
GCCCATGGCA ACTCCTCGGT GCTGGCAGAC AAGCTGGCTT TGAAGTTGGT CGGACGGGAC 
GGCTTTGTGG TGACAGAAGC TGGTTTTGGA GCAGACATCG GCATGGAGAA GTTCTTCAAC 
ATCAAATGCC GTGCTTCAGG GCTGAGGCCG AATGTGGTGG TGCTGGTCGC AACTGTCCGA 
GCCTTAAAGA TGCATGGTGG TGGCCCAAAT GTGTCAGCAG GAGCACCTCT TCCCAGAGAG 
TACATTGATG AGAATCTGAG TTTAGTTGCA GGTGGTTGTC ATAGCAATCT CAGGAAGCAG 
ATCCAAATCG CTGACGTATT CGGGGTACCA GTCGTGGTTG CGCTCAATGT CTTCAAGACA 
GACACACAGG CAGAAATTGA CCTTGTGTGT CAGCTTGCAA AAGAGTGTGG AGCCTCTGAC 
GCCGTGCCAT GTCACCACTG GGCGCAGGGC GGGCGAGGTT CCATAGAGCT GGCCCAAGCT 
GTGAATGAGG CTGCCAGCAG ACCCAGCAAC TTCCAGTTCC TGTACAACAA AGAGATGCCG 
ATAGTGGAGA AGATCAGAAC AATCGCCCAA AGAGTGTATG GAGCTGATGA CATTGAGCTG 
TCTCCAGAGG CCGGGGCCAA GATAGATTAC TACAATCAAC AGGGCTACGG AACATTGCCC 
ATCTGCATGG CCAAGACTCA CCTGTCCCTG TCCCACATGC CAGACAAGAA GGGTGCACCC 
ACTGGGTTTG TTTTGCCAAT CAGAGATGTC CGTGCTAGCA TTGGCGCTGG CTTCATCTAC 
CCACTTGTGG GAACGATGAG CACCATGCCT GGTCTGCCAA CCCGACCCTG TTTCTATGAC 
ATTGACCTTG ATCCGGTCAC GGAGGAGATC ACTGGGCTCT TCTGA