ncstn cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ncstn
Entrez Gene ID	108280450
Full Name	nicastrin
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017495411.1	XP_017350900.1	nicastrin

Pathways from BioSystems
Gene Ontology

The following ncstn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ncstn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc18229	XM_017495411.1 Latest version!	Ictalurus punctatus nicastrin (ncstn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ncstn ( XM_017495411.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> XP_017350900.1 Ictalurus punctatus nicastrin (ncstn), mRNA.

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CloneID	OIc18229
Clone ID Related Accession (Same CDS sequence)	XM_017495411.1
Accession Version	XM_017495411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-05
Organism	Ictalurus punctatus(channel catfish)
Product	nicastrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030435.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictalurus punctatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTCC TTTGTTTTGT CAGAAGCCCA GAAAAAACCA GGAAGATGGG ACCGAAATCC 
TCAAAATGGA TACAGTTGTT GCTCATTTGT TTCTTTTATT CACATGTAAG CTGTAGCTCT 
GTGGAGCAGA AGATCTATGT GGTAGTGAAC AAGACGACTC CTTGTGTTCG GCTTCTCAAT 
GCCACCCATC AGATCGGATG TCAGTCGTCC ATATCAGGAG ACACAGGGGT TCTCCATGTG 
CTGGAGACGC CAGACGATCT GGACTGGATC ACCGAAACGG GACCTCACGC TCCATACATG 
GTGCTCATGG AGACGCCTGC CTTCACCAGA TCCACGATGA TGAGGCTGAA GAACTCCACC 
CGGGTTGCAG GAGTGGCGGT CATTCACACG AGCACACCTC CAGATAAGGG GTTCTCTCCT 
CACACGTCGT GCCCCAACCA GAACACAGGA GTGTACACTC AGAGCTACGG CCCAGAACTG 
GCCAACTGCA GCACTACAGT GTGGAACCCT CTGGGTAACG GCTTGTCCTA TGAAGACTTC 
CCTTTCCCTA TTTTCTCTCT CAAAGATGAC AATGAAACAC AAGTCATTCG CAAGTGTTAC 
GAGGATCACA ACATGCGTGT GAATGGCAGT GCACCTGAAT ATCCACTGTG TGCCATGCAG 
CTCTTCTCTC ACATGCACGC AGTCACAGAC ACAGTGACCT GCATGCGACG CACGGACCTG 
CAGAACAGCT TCAGCATCAA CCCAGAGCAG CTGTGCGATC CCCTAATGGA CTACAACGTG 
TGGGCGACCA TACGACCCAT TAACGCTACC GCCAAGGGAC ACAAGGCGAA CGAGAGCATG 
GTCATTGCAG CAACCAGATT GGACGGCCGA TCCTTTTTCT ATGATGTCGC TCCATCCGCA 
GAGGGAACAG TCTCGGGCAT CGTGACACTG TTGGCCGCCG CCGAGGCCCT CCGCTCTGTC 
ACTCAAGTGG CGCCACCCCC ACGCAATATT TTCTTTGCCT TTTTCCAGGG GGAAACATTT 
GATTACATCG GTAGCTCCCG GATGGTGTAC GATATGCAGA ACAATAAATC AGTGATTGAT 
TTTGACAACG TGCACTCGTT TCTGGAGATC GGGCAGGTGG GGCTGTGGAA TAAACAGAGC 
CTATGGATGC ATTCGGATCC CGTATCTCGG AGGAACGACA CAGTGAATGA GGAGGTTTCA 
TCTCTGGTGG CCAATATCAA GTCTGCAGCC TCCATGTTGA ATCTCTCATT GGACGAACCA 
AATTCCACTC AGGCCTTACC TCCCTCCTCC TTTCAGCGCT TCCTGAGAGC TCGGCCAATC 
CCGGGAGTTG TGCTGGCAGA CCATCAATCC TCGTTTGTCA ACAGGTATTA TGAGAGTCTG 
TATGACACGT CAGAGTACCT GAACGTCAGC TATCCTCCCA ACCTAAGCCC AGAGGAGCAG 
CTAGAGTATG AGACAGAGGC TGCCAAGTCC CTGACTGAAG TGGCTACTCT GGTAGCTCGC 
TCACTGTATA AACAGGCCGG AGGGGAAGAC GGCATGCTCA AAAACATCAC TGCTGATACA 
AAGACTATGG CGAAGTTGCT GTATGGCTTT CTGGTTAAGT CCAATAACAG CTGGTTCCGT 
TCCCTGCTGC CCCGTGACTT GGTGTCAAGG GAGCGCAATC CCATATTAGA GTCAGGCCCT 
CCTCAGTACT ATGTCAGTCT GTCGCAAAGC CAACCAAAAA GTGTGACCAC CTTTGTGAAG 
TACATTCTGG CCAATATGAC TGGCACTGCC ACCCGCCTGA ACCAGAGTCA GTGCCAGAAT 
CCGAAAGACA TTTCCGGAGA GAGCAAAGAT CTCTACTCAT ACGATTGGGT GTCTGGCCCT 
GCGTCAGAGA ACCACACAAC AGGGGAACCT TTTTGTGTGC GCGCACCTGT GCGCCTGGCA 
AAAGCAGAGT CCCCCGCCTT CGAGCTGCGG CAGTATGGTT CCAGGGAGTA CTCCACATGG 
ACAGAGTCAC GCTGGAAGAA CATCGGAGCT CGTATCTTTC TAGTCGCAAG TCCACAATTA 
CAGATGCTGA CTCTAGGGGT GGGCATAGCT GTTTTGCTGG TGTCTCTAGT GGTGACGTAC 
TTCATCAGCT CAAAGGCAGA CATTTTGTTC AGTTCTACAC ATGATGCCCC AAGCACCACC 
TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017350900.1
CDS	9..2174
Translation	MDFLCFVRSPEKTRKMGPKSSKWIQLLLICFFYSHVSCSSVEQKIYVVVNKTTPCVRLLNATHQIGCQSSISGDTGVLHVLETPDDLDWITETGPHAPYMVLMETPAFTRSTMMRLKNSTRVAGVAVIHTSTPPDKGFSPHTSCPNQNTGVYTQSYGPELANCSTTVWNPLGNGLSYEDFPFPIFSLKDDNETQVIRKCYEDHNMRVNGSAPEYPLCAMQLFSHMHAVTDTVTCMRRTDLQNSFSINPEQLCDPLMDYNVWATIRPINATAKGHKANESMVIAATRLDGRSFFYDVAPSAEGTVSGIVTLLAAAEALRSVTQVAPPPRNIFFAFFQGETFDYIGSSRMVYDMQNNKSVIDFDNVHSFLEIGQVGLWNKQSLWMHSDPVSRRNDTVNEEVSSLVANIKSAASMLNLSLDEPNSTQALPPSSFQRFLRARPIPGVVLADHQSSFVNRYYESLYDTSEYLNVSYPPNLSPEEQLEYETEAAKSLTEVATLVARSLYKQAGGEDGMLKNITADTKTMAKLLYGFLVKSNNSWFRSLLPRDLVSRERNPILESGPPQYYVSLSQSQPKSVTTFVKYILANMTGTATRLNQSQCQNPKDISGESKDLYSYDWVSGPASENHTTGEPFCVRAPVRLAKAESPAFELRQYGSREYSTWTESRWKNIGARIFLVASPQLQMLTLGVGIAVLLVSLVVTYFISSKADILFSSTHDAPSTTY

Target ORF information:

RefSeq Version	XM_017495411.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus nicastrin (ncstn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017495411.1

ORF Insert Sequence:

ATGGACTTCC TTTGTTTTGT CAGAAGCCCA GAAAAAACCA GGAAGATGGG ACCGAAATCC 
TCAAAATGGA TACAGTTGTT GCTCATTTGT TTCTTTTATT CACATGTAAG CTGTAGCTCT 
GTGGAGCAGA AGATCTATGT GGTAGTGAAC AAGACGACTC CTTGTGTTCG GCTTCTCAAT 
GCCACCCATC AGATCGGATG TCAGTCGTCC ATATCAGGAG ACACAGGGGT TCTCCATGTG 
CTGGAGACGC CAGACGATCT GGACTGGATC ACCGAAACGG GACCTCACGC TCCATACATG 
GTGCTCATGG AGACGCCTGC CTTCACCAGA TCCACGATGA TGAGGCTGAA GAACTCCACC 
CGGGTTGCAG GAGTGGCGGT CATTCACACG AGCACACCTC CAGATAAGGG GTTCTCTCCT 
CACACGTCGT GCCCCAACCA GAACACAGGA GTGTACACTC AGAGCTACGG CCCAGAACTG 
GCCAACTGCA GCACTACAGT GTGGAACCCT CTGGGTAACG GCTTGTCCTA TGAAGACTTC 
CCTTTCCCTA TTTTCTCTCT CAAAGATGAC AATGAAACAC AAGTCATTCG CAAGTGTTAC 
GAGGATCACA ACATGCGTGT GAATGGCAGT GCACCTGAAT ATCCACTGTG TGCCATGCAG 
CTCTTCTCTC ACATGCACGC AGTCACAGAC ACAGTGACCT GCATGCGACG CACGGACCTG 
CAGAACAGCT TCAGCATCAA CCCAGAGCAG CTGTGCGATC CCCTAATGGA CTACAACGTG 
TGGGCGACCA TACGACCCAT TAACGCTACC GCCAAGGGAC ACAAGGCGAA CGAGAGCATG 
GTCATTGCAG CAACCAGATT GGACGGCCGA TCCTTTTTCT ATGATGTCGC TCCATCCGCA 
GAGGGAACAG TCTCGGGCAT CGTGACACTG TTGGCCGCCG CCGAGGCCCT CCGCTCTGTC 
ACTCAAGTGG CGCCACCCCC ACGCAATATT TTCTTTGCCT TTTTCCAGGG GGAAACATTT 
GATTACATCG GTAGCTCCCG GATGGTGTAC GATATGCAGA ACAATAAATC AGTGATTGAT 
TTTGACAACG TGCACTCGTT TCTGGAGATC GGGCAGGTGG GGCTGTGGAA TAAACAGAGC 
CTATGGATGC ATTCGGATCC CGTATCTCGG AGGAACGACA CAGTGAATGA GGAGGTTTCA 
TCTCTGGTGG CCAATATCAA GTCTGCAGCC TCCATGTTGA ATCTCTCATT GGACGAACCA 
AATTCCACTC AGGCCTTACC TCCCTCCTCC TTTCAGCGCT TCCTGAGAGC TCGGCCAATC 
CCGGGAGTTG TGCTGGCAGA CCATCAATCC TCGTTTGTCA ACAGGTATTA TGAGAGTCTG 
TATGACACGT CAGAGTACCT GAACGTCAGC TATCCTCCCA ACCTAAGCCC AGAGGAGCAG 
CTAGAGTATG AGACAGAGGC TGCCAAGTCC CTGACTGAAG TGGCTACTCT GGTAGCTCGC 
TCACTGTATA AACAGGCCGG AGGGGAAGAC GGCATGCTCA AAAACATCAC TGCTGATACA 
AAGACTATGG CGAAGTTGCT GTATGGCTTT CTGGTTAAGT CCAATAACAG CTGGTTCCGT 
TCCCTGCTGC CCCGTGACTT GGTGTCAAGG GAGCGCAATC CCATATTAGA GTCAGGCCCT 
CCTCAGTACT ATGTCAGTCT GTCGCAAAGC CAACCAAAAA GTGTGACCAC CTTTGTGAAG 
TACATTCTGG CCAATATGAC TGGCACTGCC ACCCGCCTGA ACCAGAGTCA GTGCCAGAAT 
CCGAAAGACA TTTCCGGAGA GAGCAAAGAT CTCTACTCAT ACGATTGGGT GTCTGGCCCT 
GCGTCAGAGA ACCACACAAC AGGGGAACCT TTTTGTGTGC GCGCACCTGT GCGCCTGGCA 
AAAGCAGAGT CCCCCGCCTT CGAGCTGCGG CAGTATGGTT CCAGGGAGTA CTCCACATGG 
ACAGAGTCAC GCTGGAAGAA CATCGGAGCT CGTATCTTTC TAGTCGCAAG TCCACAATTA 
CAGATGCTGA CTCTAGGGGT GGGCATAGCT GTTTTGCTGG TGTCTCTAGT GGTGACGTAC 
TTCATCAGCT CAAAGGCAGA CATTTTGTTC AGTTCTACAC ATGATGCCCC AAGCACCACC 
TATTGA