dhtkd1 cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dhtkd1
Entrez Gene ID	108275231
Full Name	dehydrogenase E1 and transketolase domain containing 1
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017485892.1	XP_017341381.1	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial

Pathways from BioSystems
Gene Ontology

The following dhtkd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dhtkd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc09040	XM_017485892.1 Latest version!	Ictalurus punctatus dehydrogenase E1 and transketolase domain containing 1 (dhtkd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dhtkd1 ( XM_017485892.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> XP_017341381.1 Ictalurus punctatus dehydrogenase E1 and transketolase domain containing 1 (dhtkd1), mRNA.

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CloneID	OIc09040
Clone ID Related Accession (Same CDS sequence)	XM_017485892.1
Accession Version	XM_017485892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-05
Organism	Ictalurus punctatus(channel catfish)
Product	probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictalurus punctatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCCG GTGTTATGCT AAAGAGTTTA CATAAGGTTA GTAGATATTC GTTGGCGGTG 
TCGAGGGCGC CGTTAGTAGC TTCCTACCAC ACGGAGAGAG GAGTTTTTGG GTACAGACCG 
CGGAGAGGAA ACTCGGAGGA CCCGGACGCA GAGCTGCACG CGCTGCTGAA CACACACTCC 
TGTCTCAATC AAGACCATGG CCTGGCTCGG TTGGTGGAGG CCTATCGCAC ACATGGGCAC 
AAAGCTGCTA AAATTAACCC TTTAATGCCC AATGCACCTC TTCTGGACAA AGTACCAGAG 
ATCAGCATCC TCAATGGCAG CCTCCCAGGA ACCCTTAGAA CATCTGGTTT GAGGCACTTT 
GGGAAAGTGG AGGCCAGGAC GGAGGAGGTG GTTGCGTACT TGGAGGACAC CTATTGCGGG 
AGGGTCTCTG TGGAGACCAG TCAGCTGCAG AGTCTGGAGG AGAGCGTGTG GCTCACGGAT 
CGCTTTGAGG AGCTCAAGAA GGAGAGCTTC ACTGCTGAAG AGAGGAGGCA GCTGGCGAAA 
CTCATGCTGG AATCACAGGA ATTTGACCAC TTTCTAGGCA CCAAGTTTGC CACAGTGAAG 
CGGTACGGAG GAGAGGGAGC AGAGAGCATG ATGGGATTTT TCTTTGAGCT TTTTCGTTCA 
GCAGCAAAGG GTGGCGTAAG AGATGTTGTA ATGGGAATGC CTCACCGTGG ACGCCTTAAC 
TTGCTTACTG GACTTCTGCG TTTCCCACCC GAGTTGATGT TTCGTAAAAT GAGAGGTCTC 
AGCGAATTTC CCGAGAACTC TCCCGCCATC GGAGATGTCC TCTCTCACCT CACCTCTTCT 
GTGGACCTGG AAATGGGCAC TGACCACCCC CTCCACGTGA CCATGCTACC CAACCCATCC 
CATTTGGAGG CCATTAACCC GGTGGCTCAG GGGAAAACGC GTGGCCGACA ACAAGTCAAA 
CAGGATGGGG ATTATTCTAA CGACAGCCAA GCTAGGCCTG GAGACAAAGT CATCTGTTTG 
CAGGTTCACG GTGATGCCTC ATTCTCAGGG CAAGGAATTG TGCCAGAAAC ATTCACCCTG 
TCAAATCTCC CTCACTTCAG AGTTGGTGGA AGCATCCATC TTATTGTGAA CAATCAAGTG 
GGCTATACCA CTCCCTCTGA GAGAGCCCGC TCGTCCTTGT ACTGCAGTGA TATTGGTAAA 
ATGGTGGGTT GTGCTGTGAT CCATGTAAAC GGCGATGATG CTGAAGACGT TCTCAGAGCC 
ACACGGTTAG CTGTGGAGTA TCAAAGACGC TTCAGGAAAG ACGTTATAGT GGAGCTGCTG 
TGTTACCGGC AGTGGGGTCA CAACGAACTG GACGAGCCAT TTTTCACAAA CCCTGCCATG 
TACAAAATCA TCCGCTCAAG GAAGAGCGTC CCTGATTCCT ACGCTGACCA GCTGATCTCT 
GAGGGATTGA TGACTGAGGA AGAACGCAGT CAGATTAAAA CAGCCTACTA CTCCTTGCTC 
AGTGATCGTC TGGCCAACAT GACCTTCTAT AGCCCCCCAC CCACCAACCT GCAGGGCCGC 
TGGGGAGATC TGATGGAGCC ACAGAACAGA GTTTCAGCCT GGGACACTGG CGTGGCTGTT 
CCGTTGCTTC AGTATGTCGG AGCAAAGTCA GTGGAGATCC CTGAGGAGGT CCAGGTGCAC 
AGTCACCTTG TGAAGACGCA TGTAAAGTCG AGACTGCAGA AACTTGAAGC GGGAACTGCA 
CTGGACTGGT CCACTGCTGA GGCGCTGGCG TTCGGCACAC TTCTCTGTCA GGGTTTCGAC 
GTGCGGATAA GCGGCCAGGA TGTTGGCCGT GGTACATTTA GTCAGCGTCA TGCCATGGTT 
GTGTGCCAGG AGTCCAATGA CATGTATATC CCTCTCAATC ACATTGACCC TCAACAAAAA 
GGCCATCTGG AGGTGTGTAA CAGTGCACTG TCTGAAGAGG CAGTGTTAGG TTTTGAGTAC 
GGGATGAGTA TTGAACAGCC AAAACTGCTG CCAATATGGG AAGCTCAGTT CGGGGATTTC 
TTCAATGGAG CGCAAATCAT CTTTGACACC TTTATCTCTG GAGGTGAGGC AAAGTGGTTG 
CTGCAGAGCG GCATGGTCAT CCTGCTTCCA CACGGCTACG ACGGAGCCGG ACCCGAGCAC 
TCGTCCTGCC GCATCGAGCG TTTCTTACAA CTCTGTGACA GTAAGGAGGA AGGCATCGAC 
GGTGACGTAG TCAATATGGC CGTGGTGAAT CCGACAACTC CGGCTCAGTA CTTCCACCTG 
TTGAGGAGAC AAATGATCCG AAACTTCCGC AAACCACTCA TTGTGGCCTC ACCCAAAACT 
CTGTTGCGTT TTTCTGGGGC AGTGTCGAGC TTGGAGGAAA TGGGACCTGG AACTTCTTTC 
AAGCCTGTAA TAGGCGACAC CTCTGTTAAT CCTGAGAGTG TGCAGAGAGT GCTCCTTTGC 
TCAGGCAAGC ACTATTATGC ACTGCTAAAA CAAAGAGAGA CCCTGCCTGA GGCATGTAAG 
AACACGGCAC TGATCAGAGT AGAGGAGCTT TGCCCTTTCC CCACAGACGC CTTACAGCAG 
GAGCTCCACA GATACAGCAA TGCCAAAGAG TTTATCTGGA GCCAAGAAGA GCCACAGAAC 
ATGGGATGCT GGTTTTTTGT GTCTCCTCGT TTTGAAAAAC AGCTTGCCTG TAAGCTGCGA 
TTGGTCAGTA GGCCTCCTCT GGCTGCCCCT GCTGTGGGCA TCAGTACTCT TCACCATCAG 
CAGCACGAGG CCATTCTTAC GGCCTCCTTC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017341381.1
CDS	242..3037
Translation	MSAGVMLKSLHKVSRYSLAVSRAPLVASYHTERGVFGYRPRRGNSEDPDAELHALLNTHSCLNQDHGLARLVEAYRTHGHKAAKINPLMPNAPLLDKVPEISILNGSLPGTLRTSGLRHFGKVEARTEEVVAYLEDTYCGRVSVETSQLQSLEESVWLTDRFEELKKESFTAEERRQLAKLMLESQEFDHFLGTKFATVKRYGGEGAESMMGFFFELFRSAAKGGVRDVVMGMPHRGRLNLLTGLLRFPPELMFRKMRGLSEFPENSPAIGDVLSHLTSSVDLEMGTDHPLHVTMLPNPSHLEAINPVAQGKTRGRQQVKQDGDYSNDSQARPGDKVICLQVHGDASFSGQGIVPETFTLSNLPHFRVGGSIHLIVNNQVGYTTPSERARSSLYCSDIGKMVGCAVIHVNGDDAEDVLRATRLAVEYQRRFRKDVIVELLCYRQWGHNELDEPFFTNPAMYKIIRSRKSVPDSYADQLISEGLMTEEERSQIKTAYYSLLSDRLANMTFYSPPPTNLQGRWGDLMEPQNRVSAWDTGVAVPLLQYVGAKSVEIPEEVQVHSHLVKTHVKSRLQKLEAGTALDWSTAEALAFGTLLCQGFDVRISGQDVGRGTFSQRHAMVVCQESNDMYIPLNHIDPQQKGHLEVCNSALSEEAVLGFEYGMSIEQPKLLPIWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGMVILLPHGYDGAGPEHSSCRIERFLQLCDSKEEGIDGDVVNMAVVNPTTPAQYFHLLRRQMIRNFRKPLIVASPKTLLRFSGAVSSLEEMGPGTSFKPVIGDTSVNPESVQRVLLCSGKHYYALLKQRETLPEACKNTALIRVEELCPFPTDALQQELHRYSNAKEFIWSQEEPQNMGCWFFVSPRFEKQLACKLRLVSRPPLAAPAVGISTLHHQQHEAILTASFS

Target ORF information:

RefSeq Version	XM_017485892.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus dehydrogenase E1 and transketolase domain containing 1 (dhtkd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017485892.1

ORF Insert Sequence:

ATGTCTGCCG GTGTTATGCT AAAGAGTTTA CATAAGGTTA GTAGATATTC GTTGGCGGTG 
TCGAGGGCGC CGTTAGTAGC TTCCTACCAC ACGGAGAGAG GAGTTTTTGG GTACAGACCG 
CGGAGAGGAA ACTCGGAGGA CCCGGACGCA GAGCTGCACG CGCTGCTGAA CACACACTCC 
TGTCTCAATC AAGACCATGG CCTGGCTCGG TTGGTGGAGG CCTATCGCAC ACATGGGCAC 
AAAGCTGCTA AAATTAACCC TTTAATGCCC AATGCACCTC TTCTGGACAA AGTACCAGAG 
ATCAGCATCC TCAATGGCAG CCTCCCAGGA ACCCTTAGAA CATCTGGTTT GAGGCACTTT 
GGGAAAGTGG AGGCCAGGAC GGAGGAGGTG GTTGCGTACT TGGAGGACAC CTATTGCGGG 
AGGGTCTCTG TGGAGACCAG TCAGCTGCAG AGTCTGGAGG AGAGCGTGTG GCTCACGGAT 
CGCTTTGAGG AGCTCAAGAA GGAGAGCTTC ACTGCTGAAG AGAGGAGGCA GCTGGCGAAA 
CTCATGCTGG AATCACAGGA ATTTGACCAC TTTCTAGGCA CCAAGTTTGC CACAGTGAAG 
CGGTACGGAG GAGAGGGAGC AGAGAGCATG ATGGGATTTT TCTTTGAGCT TTTTCGTTCA 
GCAGCAAAGG GTGGCGTAAG AGATGTTGTA ATGGGAATGC CTCACCGTGG ACGCCTTAAC 
TTGCTTACTG GACTTCTGCG TTTCCCACCC GAGTTGATGT TTCGTAAAAT GAGAGGTCTC 
AGCGAATTTC CCGAGAACTC TCCCGCCATC GGAGATGTCC TCTCTCACCT CACCTCTTCT 
GTGGACCTGG AAATGGGCAC TGACCACCCC CTCCACGTGA CCATGCTACC CAACCCATCC 
CATTTGGAGG CCATTAACCC GGTGGCTCAG GGGAAAACGC GTGGCCGACA ACAAGTCAAA 
CAGGATGGGG ATTATTCTAA CGACAGCCAA GCTAGGCCTG GAGACAAAGT CATCTGTTTG 
CAGGTTCACG GTGATGCCTC ATTCTCAGGG CAAGGAATTG TGCCAGAAAC ATTCACCCTG 
TCAAATCTCC CTCACTTCAG AGTTGGTGGA AGCATCCATC TTATTGTGAA CAATCAAGTG 
GGCTATACCA CTCCCTCTGA GAGAGCCCGC TCGTCCTTGT ACTGCAGTGA TATTGGTAAA 
ATGGTGGGTT GTGCTGTGAT CCATGTAAAC GGCGATGATG CTGAAGACGT TCTCAGAGCC 
ACACGGTTAG CTGTGGAGTA TCAAAGACGC TTCAGGAAAG ACGTTATAGT GGAGCTGCTG 
TGTTACCGGC AGTGGGGTCA CAACGAACTG GACGAGCCAT TTTTCACAAA CCCTGCCATG 
TACAAAATCA TCCGCTCAAG GAAGAGCGTC CCTGATTCCT ACGCTGACCA GCTGATCTCT 
GAGGGATTGA TGACTGAGGA AGAACGCAGT CAGATTAAAA CAGCCTACTA CTCCTTGCTC 
AGTGATCGTC TGGCCAACAT GACCTTCTAT AGCCCCCCAC CCACCAACCT GCAGGGCCGC 
TGGGGAGATC TGATGGAGCC ACAGAACAGA GTTTCAGCCT GGGACACTGG CGTGGCTGTT 
CCGTTGCTTC AGTATGTCGG AGCAAAGTCA GTGGAGATCC CTGAGGAGGT CCAGGTGCAC 
AGTCACCTTG TGAAGACGCA TGTAAAGTCG AGACTGCAGA AACTTGAAGC GGGAACTGCA 
CTGGACTGGT CCACTGCTGA GGCGCTGGCG TTCGGCACAC TTCTCTGTCA GGGTTTCGAC 
GTGCGGATAA GCGGCCAGGA TGTTGGCCGT GGTACATTTA GTCAGCGTCA TGCCATGGTT 
GTGTGCCAGG AGTCCAATGA CATGTATATC CCTCTCAATC ACATTGACCC TCAACAAAAA 
GGCCATCTGG AGGTGTGTAA CAGTGCACTG TCTGAAGAGG CAGTGTTAGG TTTTGAGTAC 
GGGATGAGTA TTGAACAGCC AAAACTGCTG CCAATATGGG AAGCTCAGTT CGGGGATTTC 
TTCAATGGAG CGCAAATCAT CTTTGACACC TTTATCTCTG GAGGTGAGGC AAAGTGGTTG 
CTGCAGAGCG GCATGGTCAT CCTGCTTCCA CACGGCTACG ACGGAGCCGG ACCCGAGCAC 
TCGTCCTGCC GCATCGAGCG TTTCTTACAA CTCTGTGACA GTAAGGAGGA AGGCATCGAC 
GGTGACGTAG TCAATATGGC CGTGGTGAAT CCGACAACTC CGGCTCAGTA CTTCCACCTG 
TTGAGGAGAC AAATGATCCG AAACTTCCGC AAACCACTCA TTGTGGCCTC ACCCAAAACT 
CTGTTGCGTT TTTCTGGGGC AGTGTCGAGC TTGGAGGAAA TGGGACCTGG AACTTCTTTC 
AAGCCTGTAA TAGGCGACAC CTCTGTTAAT CCTGAGAGTG TGCAGAGAGT GCTCCTTTGC 
TCAGGCAAGC ACTATTATGC ACTGCTAAAA CAAAGAGAGA CCCTGCCTGA GGCATGTAAG 
AACACGGCAC TGATCAGAGT AGAGGAGCTT TGCCCTTTCC CCACAGACGC CTTACAGCAG 
GAGCTCCACA GATACAGCAA TGCCAAAGAG TTTATCTGGA GCCAAGAAGA GCCACAGAAC 
ATGGGATGCT GGTTTTTTGT GTCTCCTCGT TTTGAAAAAC AGCTTGCCTG TAAGCTGCGA 
TTGGTCAGTA GGCCTCCTCT GGCTGCCCCT GCTGTGGGCA TCAGTACTCT TCACCATCAG 
CAGCACGAGG CCATTCTTAC GGCCTCCTTC TCCTGA